Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 625,174 G→T 100% intergenic (+5/‑176) entH → / → cstA enterobactin synthesis proofreading thioesterase/carbon starvation protein involved in peptide utilization; APC peptide transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273625,1740GT98.6% 665.8 / 0.3 214intergenic (+5/‑176)entH/cstAenterobactin synthesis proofreading thioesterase/carbon starvation protein involved in peptide utilization; APC peptide transporter family protein
Reads supporting (aligned to +/- strand):  ref base G (3/0);  new base T (103/108);  total (106/108)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 214 total aligned reads displayed.

GCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACAT  >  CP009273/625027‑625320
                                                                                                                                                   |                                                                                                                                                  
gCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTAc                                                                                                                                                  >  1:926263/1‑150 (MQ=255)
gCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTAc                                                                                                                                                  >  2:3453837/1‑150 (MQ=255)
  ccGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAg                                                                                                                                                <  1:1581239/150‑1 (MQ=255)
  ccGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAg                                                                                                                                                >  1:3010744/1‑150 (MQ=255)
  ccGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAg                                                                                                                                                <  2:4002920/150‑1 (MQ=255)
   cGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGAAGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGa                                                                                                                                               >  1:1141533/1‑150 (MQ=255)
      tgtCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAAtt                                                                                                                                            <  1:409708/150‑1 (MQ=255)
       gtCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTa                                                                                                                                           <  1:1018996/150‑1 (MQ=255)
       gtCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTa                                                                                                                                           >  1:471936/1‑150 (MQ=255)
       gtCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTa                                                                                                                                           <  2:4167962/150‑1 (MQ=255)
           gAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATg                                                                                                                                       >  1:3642377/1‑150 (MQ=255)
             gggAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATgag                                                                                                                                     <  1:2743391/150‑1 (MQ=255)
              ggAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATgaga                                                                                                                                    >  2:2340941/1‑150 (MQ=255)
               gAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAt                                                                                                                                   >  2:3029623/1‑150 (MQ=255)
                aaaGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAtt                                                                                                                                  >  1:1927535/1‑150 (MQ=255)
                       cgcgGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGaa                                                                                                                           <  2:4261487/150‑1 (MQ=255)
                         cggcgTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGaaaa                                                                                                                         >  1:3183674/1‑150 (MQ=255)
                               cTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGt                                                                                                                   <  2:388401/150‑1 (MQ=255)
                                 gCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTa                                                                                                                 >  2:109065/1‑150 (MQ=255)
                                 gCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTa                                                                                                                 >  1:509626/1‑150 (MQ=255)
                                 gCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTa                                                                                                                 >  2:5120340/1‑150 (MQ=255)
                                  ccAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTaa                                                                                                                >  2:3985454/1‑150 (MQ=255)
                                  ccAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTaa                                                                                                                >  2:2489272/1‑150 (MQ=255)
                                   cAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAAc                                                                                                               <  2:2124499/150‑1 (MQ=255)
                                    aGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACg                                                                                                              >  1:3272849/1‑150 (MQ=255)
                                     gCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACgg                                                                                                             <  1:3034162/150‑1 (MQ=255)
                                      ccGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGa                                                                                                            >  1:2357997/1‑150 (MQ=255)
                                         cTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTg                                                                                                         <  1:909933/150‑1 (MQ=255)
                                          tGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGa                                                                                                        >  2:3286093/1‑150 (MQ=255)
                                          tGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGa                                                                                                        >  2:3123794/1‑150 (MQ=255)
                                            cATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATc                                                                                                      <  1:3969592/150‑1 (MQ=255)
                                            cATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATc                                                                                                      <  2:3400938/150‑1 (MQ=255)
                                            cATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATc                                                                                                      <  1:2519662/150‑1 (MQ=255)
                                             aTCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCg                                                                                                     <  1:4919728/150‑1 (MQ=255)
                                             aTCTTGGTCGGCAAAATCAGAGCAGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACACGGTTAACGGAGTGATCg                                                                                                     >  1:2869766/1‑150 (MQ=255)
                                              tCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGa                                                                                                    <  2:1521779/150‑1 (MQ=255)
                                                 tGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGtt                                                                                                 <  2:840649/150‑1 (MQ=255)
                                                 tGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGtt                                                                                                 <  1:1448710/150‑1 (MQ=255)
                                                   gTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTaa                                                                                               >  2:3629488/1‑150 (MQ=255)
                                                     cGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACa                                                                                             >  1:4286859/1‑150 (MQ=255)
                                                       gCAAACTCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACAtt                                                                                           <  2:1741906/150‑1 (MQ=255)
                                                           aaTCAGAGCTGGGAAATCGTCTTTTTAGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTTGGATGAACCCTACAGAATTAGAAGAGATTGCAGGAAAAATCGGTTAACGGAGTGATCGAGTTAACATTgtta                                                                                       >  2:151010/1‑150 (MQ=255)
                                                           aaTCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgtta                                                                                       >  2:4316915/1‑150 (MQ=255)
                                                             tCAGAGCTGGGAAATCGTCGTTTTCGATGACCAGGGGCGGCGGTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAAGTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgttaag                                                                                     <  2:898827/150‑1 (MQ=255)
                                                             tCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgttaag                                                                                     <  2:3698532/150‑1 (MQ=255)
                                                             tCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgttaag                                                                                     >  2:1840628/1‑150 (MQ=255)
                                                               agagCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgttaagtt                                                                                   >  2:832833/1‑150 (MQ=255)
                                                                 agCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACAGTgttaagttaa                                                                                 >  2:1357456/1‑150 (MQ=255)
                                                                    tGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAata                                                                              >  1:63799/1‑150 (MQ=255)
                                                                     gggAAATCGTCGTTTTCGATGAACATGGGCGGCGTTGCTGCACTTGTCGGCTGGGTAAGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACAGTGTTAAGTTAAatat                                                                             >  1:560653/1‑150 (MQ=255)
                                                                     gggAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAatat                                                                             <  1:2423686/150‑1 (MQ=255)
                                                                     gggAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAatat                                                                             >  2:476842/1‑150 (MQ=255)
                                                                        aaaTCGTCGTTTTCGATGATCAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACTGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTgg                                                                          <  2:1043666/150‑1 (MQ=255)
                                                                        aaaTCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTgg                                                                          <  2:2549084/150‑1 (MQ=255)
                                                                           tcgtcgTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGttt                                                                       >  1:871712/1‑150 (MQ=255)
                                                                           tcgtcgTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGttt                                                                       >  2:2307604/1‑150 (MQ=255)
                                                                            cgtcgtTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTc                                                                      >  1:761130/1‑150 (MQ=255)
                                                                            cgtcgtTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTc                                                                      >  1:3908842/1‑150 (MQ=255)
                                                                            cgtcgtTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTc                                                                      >  2:3078098/1‑150 (MQ=255)
                                                                             gtcgtTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCa                                                                     >  2:4678540/1‑150 (MQ=255)
                                                                             gtcgtTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCa                                                                     <  2:2164487/150‑1 (MQ=255)
                                                                               cgtTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAAc                                                                   >  2:2788922/1‑150 (MQ=255)
                                                                                 ttttCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTc                                                                 >  2:1555109/1‑150 (MQ=255)
                                                                                 ttttCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTc                                                                 >  1:16862/1‑150 (MQ=255)
                                                                                  tttCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTcc                                                                <  2:1927444/150‑1 (MQ=255)
                                                                                     cGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCTAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGAt                                                             <  2:1485792/150‑1 (MQ=255)
                                                                                       aTGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGAttt                                                           >  2:2720070/1‑150 (MQ=255)
                                                                                        tGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTa                                                          <  2:2776872/150‑1 (MQ=255)
                                                                                        tGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTa                                                          <  2:4931939/150‑1 (MQ=255)
                                                                                        tGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTa                                                          <  2:4728390/150‑1 (MQ=255)
                                                                                        tGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTa                                                          <  2:4573010/150‑1 (MQ=255)
                                                                                         gAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTAc                                                         >  2:402383/1‑150 (MQ=255)
                                                                                           aCAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACAt                                                       <  1:3250902/150‑1 (MQ=255)
                                                                                             aGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATgg                                                     >  1:4441876/1‑150 (MQ=255)
                                                                                               gggCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGtt                                                   <  1:4828271/150‑1 (MQ=255)
                                                                                               gggCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGtt                                                   >  2:3268072/1‑150 (MQ=255)
                                                                                                   ggcgTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCtg                                               <  2:1175957/150‑1 (MQ=255)
                                                                                                      gTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTgtgt                                            <  1:1199335/150‑1 (MQ=255)
                                                                                                       ttGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGtttt                                           >  1:4990735/1‑150 (MQ=255)
                                                                                                         gctgcACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGtgttgt                                         >  2:923098/1‑150 (MQ=255)
                                                                                                         gctgcACTTGTCGGCTGGGAACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGtgttgt                                         >  1:966353/1‑150 (MQ=255)
                                                                                                          ctgcACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTgttgtt                                        >  1:1542556/1‑150 (MQ=255)
                                                                                                          ctgcACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTgttgtt                                        <  2:1293856/150‑1 (MQ=255)
                                                                                                          ctgcACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTgttgtt                                        <  1:3727287/150‑1 (MQ=255)
                                                                                                                ttGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTg                                  <  2:823270/150‑1 (MQ=255)
                                                                                                                  gTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTa                                <  1:1291760/150‑1 (MQ=255)
                                                                                                                     ggCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACaa                             >  2:4388855/1‑150 (MQ=255)
                                                                                                                       cTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATGAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAg                           <  2:2934751/150‑1 (MQ=255)
                                                                                                                          ggTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATg                        <  1:4516199/150‑1 (MQ=255)
                                                                                                                            tACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGtt                      <  2:4522677/150‑1 (MQ=255)
                                                                                                                             aCGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTta                     <  1:3453837/150‑1 (MQ=255)
                                                                                                                               ggCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTtata                   >  1:2102386/1‑150 (MQ=255)
                                                                                                                               ggCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTtata                   >  1:2452270/1‑150 (MQ=255)
                                                                                                                                   gTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGaaa               <  1:3540529/150‑1 (MQ=255)
                                                                                                                                   gTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGaaa               >  1:5120710/1‑150 (MQ=255)
                                                                                                                                      ttGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCTAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACaa            >  1:4388624/1‑150 (MQ=255)
                                                                                                                                         ggATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAAt         >  2:803014/1‑150 (MQ=255)
                                                                                                                                           aTGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGt       <  2:647657/150‑1 (MQ=255)
                                                                                                                                            tGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTa      >  1:2225631/1‑150 (MQ=255)
                                                                                                                                              aaCCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAAc    <  2:1893155/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACAT  >  CP009273/625027‑625320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCTC  >  CP009273/625026‑625323
                                                                                                                                                    |                                                                                                                                                     
CGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATTAACCCTA                                                                                                                                                      >  SRR19973063.4743459/1‑150 (MQ=60)
CGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTA                                                                                                                                                      >  SRR19973063.4587638/1‑150 (MQ=60)
CGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTA                                                                                                                                                      >  SRR19973063.4587633/1‑150 (MQ=60)
 GCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTAC                                                                                                                                                     >  SRR19973063.929018/1‑150 (MQ=60)
 GCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTAC                                                                                                                                                     >  SRR19973063.929016/1‑150 (MQ=60)
   CCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAG                                                                                                                                                   >  SRR19973063.4383243/1‑150 (MQ=60)
   CCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAG                                                                                                                                                   <  SRR19973063.3684485/150‑1 (MQ=60)
   CCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAG                                                                                                                                                   <  SRR19973063.1586089/150‑1 (MQ=60)
   CCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAG                                                                                                                                                   >  SRR19973063.3020349/1‑150 (MQ=60)
    CGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGAAGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGA                                                                                                                                                  >  SRR19973063.1144989/1‑150 (MQ=60)
    AGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGA                                                                                                                                                  >  SRR19973063.4820629/1‑150 (MQ=60)
       TGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATT                                                                                                                                               <  SRR19973063.410861/150‑1 (MQ=60)
        GTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTA                                                                                                                                              <  SRR19973063.1022067/150‑1 (MQ=60)
        GTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTA                                                                                                                                              >  SRR19973063.473267/1‑150 (MQ=60)
           TGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGAT                                                                                                                                           <  SRR19973063.4277685/150‑1 (MQ=60)
            GAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATG                                                                                                                                          >  SRR19973063.3654064/1‑150 (MQ=60)
              GGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAG                                                                                                                                        <  SRR19973063.2752246/150‑1 (MQ=60)
              GGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAG                                                                                                                                        >  SRR19973063.2467926/1‑150 (MQ=60)
               GGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGA                                                                                                                                       >  SRR19973063.1526446/1‑150 (MQ=60)
                 AAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATT                                                                                                                                     <  SRR19973063.1165827/150‑1 (MQ=60)
                 AAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATT                                                                                                                                     >  SRR19973063.1933559/1‑150 (MQ=60)
                  AAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTG                                                                                                                                    >  SRR19973063.915329/1‑150 (MQ=60)
                       ACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGGTTTAGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTATGGATGAACCCTACAGAATTAGATGAGATTGCAGGA                                                                                                                               >  SRR19973063.5150496/1‑150 (MQ=60)
                          CGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAA                                                                                                                            >  SRR19973063.3193907/1‑150 (MQ=60)
                          CGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAA                                                                                                                            >  SRR19973063.335063/1‑150 (MQ=60)
                               TCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGG                                                                                                                       <  SRR19973063.3628357/150‑1 (MQ=60)
                                  GCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTA                                                                                                                    >  SRR19973063.511105/1‑150 (MQ=60)
                                  GCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTA                                                                                                                    >  SRR19973063.510995/1‑150 (MQ=60)
                                    CAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAAC                                                                                                                  <  SRR19973063.1898872/150‑1 (MQ=60)
                                     AGNCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACG                                                                                                                 >  SRR19973063.241780/1‑150 (MQ=60)
                                     AGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACG                                                                                                                 >  SRR19973063.3283354/1‑150 (MQ=60)
                                      GCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGG                                                                                                                <  SRR19973063.800682/150‑1 (MQ=60)
                                      GCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGG                                                                                                                <  SRR19973063.3043852/150‑1 (MQ=60)
                                      GCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGG                                                                                                                <  SRR19973063.3043642/150‑1 (MQ=60)
                                       CCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACCGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGA                                                                                                               <  SRR19973063.2636292/150‑1 (MQ=60)
                                       CCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGA                                                                                                               >  SRR19973063.2365544/1‑150 (MQ=60)
                                          CTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTG                                                                                                            <  SRR19973063.912530/150‑1 (MQ=60)
                                          CTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTG                                                                                                            <  SRR19973063.912606/150‑1 (MQ=60)
                                          CTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTG                                                                                                            >  SRR19973063.1309426/1‑150 (MQ=60)
                                             CATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATC                                                                                                         <  SRR19973063.2527699/150‑1 (MQ=60)
                                             CATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATC                                                                                                         <  SRR19973063.3268620/150‑1 (MQ=60)
                                             CATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATC                                                                                                         <  SRR19973063.3982297/150‑1 (MQ=60)
                                              ATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCG                                                                                                        <  SRR19973063.2159379/150‑1 (MQ=60)
                                              ATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCG                                                                                                        <  SRR19973063.4935615/150‑1 (MQ=60)
                                              ATCTTGGTCGGCAAAATCAGAGCAGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACACGGTTAACGGAGTGATCG                                                                                                        >  SRR19973063.2878954/1‑150 (MQ=60)
                                                 TTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTGTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTAGGTTAACGGAGTGATCGAGT                                                                                                     >  SRR19973063.3514531/1‑150 (MQ=60)
                                                  TGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTT                                                                                                    <  SRR19973063.1453130/150‑1 (MQ=60)
                                                      CGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACA                                                                                                >  SRR19973063.4300479/1‑150 (MQ=60)
                                                      CGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACA                                                                                                <  SRR19973063.406514/150‑1 (MQ=60)
                                                        GCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATT                                                                                              <  SRR19973063.2021144/150‑1 (MQ=60)
                                                         CAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTG                                                                                             >  SRR19973063.708900/1‑150 (MQ=60)
                                                            AATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTTGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTA                                                                                          >  SRR19973063.3051831/1‑150 (MQ=60)
                                                                     TGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATA                                                                                 >  SRR19973063.4472554/1‑150 (MQ=60)
                                                                     TGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATA                                                                                 >  SRR19973063.63707/1‑150 (MQ=60)
                                                                     TGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATA                                                                                 >  SRR19973063.64017/1‑150 (MQ=60)
                                                                      GGGAAATCGTCGTTTTCGATGAACATGGGCGGCGTTGCTGCACTTGTCGGCTGGGTAAGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACAGTGTTAAGTTAAATAT                                                                                >  SRR19973063.562308/1‑150 (MQ=60)
                                                                      GGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATAT                                                                                <  SRR19973063.2431434/150‑1 (MQ=60)
                                                                          AATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGT                                                                            >  SRR19973063.4999095/1‑150 (MQ=60)
                                                                            TCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTT                                                                          >  SRR19973063.874216/1‑150 (MQ=60)
                                                                             CGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTC                                                                         >  SRR19973063.763348/1‑150 (MQ=60)
                                                                             CGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTC                                                                         >  SRR19973063.3921304/1‑150 (MQ=60)
                                                                                CGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAAC                                                                      >  SRR19973063.1828808/1‑150 (MQ=60)
                                                                                  TTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTC                                                                    >  SRR19973063.5002263/1‑150 (MQ=60)
                                                                                  TTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTC                                                                    >  SRR19973063.16927/1‑150 (MQ=60)
                                                                                          GAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTAA                                                            >  SRR19973063.876475/1‑150 (MQ=60)
                                                                                            ACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACAT                                                          <  SRR19973063.3261334/150‑1 (MQ=60)
                                                                                             CAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATG                                                         <  SRR19973063.1665714/150‑1 (MQ=60)
                                                                                             CAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATG                                                         <  SRR19973063.2460093/150‑1 (MQ=60)
                                                                                              AGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGG                                                        >  SRR19973063.4456128/1‑150 (MQ=60)
                                                                                              AGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGGGATCGAGGTAACATTGTTAAGGTAAATATTGGTTTCAACTCCGAGTTACATGG                                                        >  SRR19973063.2994373/1‑150 (MQ=60)
                                                                                               GGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGT                                                       <  SRR19973063.492868/150‑1 (MQ=60)
                                                                                               GGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGT                                                       <  SRR19973063.4650560/150‑1 (MQ=60)
                                                                                                GGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTT                                                      <  SRR19973063.3805211/150‑1 (MQ=60)
                                                                                                GGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTT                                                      <  SRR19973063.4843740/150‑1 (MQ=60)
                                                                                                 GGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTG                                                     >  SRR19973063.4435369/1‑150 (MQ=60)
                                                                                                       GTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGT                                               <  SRR19973063.1202935/150‑1 (MQ=60)
                                                                                                        TTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTT                                              >  SRR19973063.4146213/1‑150 (MQ=60)
                                                                                                        TTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTTTT                                              >  SRR19973063.5006855/1‑150 (MQ=60)
                                                                                                         TGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTG                                             >  SRR19973063.1056928/1‑150 (MQ=60)
                                                                                                         TGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTG                                             <  SRR19973063.2790654/150‑1 (MQ=60)
                                                                                                          GCTGCACTTGTCGGCTGGGAACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGT                                            >  SRR19973063.969268/1‑150 (MQ=60)
                                                                                                           CTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTT                                           >  SRR19973063.1547286/1‑150 (MQ=60)
                                                                                                           CTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTT                                           <  SRR19973063.1574833/150‑1 (MQ=60)
                                                                                                           CTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTT                                           <  SRR19973063.3930212/150‑1 (MQ=60)
                                                                                                           CTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTT                                           <  SRR19973063.3739198/150‑1 (MQ=60)
                                                                                                               ACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGTGTTAACATTGTTAAGTTACATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAAT                                       <  SRR19973063.3237641/150‑1 (MQ=60)
                                                                                                                   GTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTA                                   <  SRR19973063.1295608/150‑1 (MQ=60)
                                                                                                                    TCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTAC                                  >  SRR19973063.4648517/1‑150 (MQ=60)
                                                                                                                         TGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGA                             >  SRR19973063.490843/1‑150 (MQ=60)
                                                                                                                          GGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGAT                            >  SRR19973063.1272524/1‑150 (MQ=60)
                                                                                                                           GGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATG                           <  SRR19973063.4530697/150‑1 (MQ=60)
                                                                                                                              ACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTA                        <  SRR19973063.3464817/150‑1 (MQ=60)
                                                                                                                                GGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATA                      >  SRR19973063.2108961/1‑150 (MQ=60)
                                                                                                                                GGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATA                      >  SRR19973063.2460103/1‑150 (MQ=60)
                                                                                                                                    GTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAA                  <  SRR19973063.3551872/150‑1 (MQ=60)
                                                                                                                                    GTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAA                  >  SRR19973063.2719883/1‑150 (MQ=60)
                                                                                                                                    GTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAA                  >  SRR19973063.5137184/1‑150 (MQ=60)
                                                                                                                                    GTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAA                  >  SRR19973063.5137002/1‑150 (MQ=60)
                                                                                                                                       TTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCTAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAA               >  SRR19973063.4402680/1‑150 (MQ=60)
                                                                                                                                             TGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTA         >  SRR19973063.2232678/1‑150 (MQ=60)
                                                                                                                                                ACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAACGTAACA      <  SRR19973063.2314980/150‑1 (MQ=60)
                                                                                                                                                 CCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACAT     >  SRR19973063.3367446/1‑150 (MQ=60)
                                                                                                                                                  CCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATC    >  SRR19973063.5083770/1‑150 (MQ=60)
                                                                                                                                                  CCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATC    >  SRR19973063.1832698/1‑150 (MQ=60)
                                                                                                                                                  CCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATC    >  SRR19973063.583674/1‑150 (MQ=60)
                                                                                                                                                    TACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCTC  >  SRR19973063.2491625/1‑150 (MQ=60)
                                                                                                                                                    TACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCTC  >  SRR19973063.3887949/1‑150 (MQ=60)
                                                                                                                                                    TACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCTC  <  SRR19973063.3263129/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCTC  >  CP009273/625026‑625323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑