Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 625,174 G→T 86.4% intergenic (+5/‑176) entH → / → cstA enterobactin synthesis proofreading thioesterase/carbon starvation protein involved in peptide utilization; APC peptide transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273625,1740GT86.4% 560.7 / 98.5 257intergenic (+5/‑176)entH/cstAenterobactin synthesis proofreading thioesterase/carbon starvation protein involved in peptide utilization; APC peptide transporter family protein
Reads supporting (aligned to +/- strand):  ref base G (9/26);  new base T (78/139);  total (92/165)
Fisher's exact test for biased strand distribution p-value = 2.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

Only 100 of 258 total aligned reads displayed.

CGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACA  >  CP009273/625026‑625319
                                                                                                                                                    |                                                                                                                                                 
cGCCCGGGGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCCTCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCGGGGTACGGCAGTGTTGGGATGAACCCg                                                                                                                                                   >  2:576333/1‑149 (MQ=255)
   ccGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACa                                                                                                                                                <  2:956653/149‑1 (MQ=255)
    cGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAg                                                                                                                                               >  1:1570129/1‑149 (MQ=255)
     ggTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTGTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCCGTTTTGGGCTGAACCCTACAGa                                                                                                                                              >  2:3231726/1‑149 (MQ=255)
        gtCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAAtt                                                                                                                                           >  2:3691926/1‑149 (MQ=255)
        gtCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAAtt                                                                                                                                           <  2:3370823/149‑1 (MQ=255)
        gtCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAAtt                                                                                                                                           <  2:2855375/149‑1 (MQ=255)
         tCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTa                                                                                                                                          <  1:1420558/149‑1 (MQ=255)
            gAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGAt                                                                                                                                       <  1:1922201/149‑1 (MQ=255)
             aGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCAACAGAATTAGATg                                                                                                                                      >  1:201019/1‑149 (MQ=255)
                gAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATgaga                                                                                                                                   <  1:653388/149‑1 (MQ=255)
                gAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATgaga                                                                                                                                   <  1:622463/149‑1 (MQ=255)
                 aaaGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGGTGCTGCACGTGTCGGCTGGGTACGGCAGTTTTGGGGTGTACCCTACAGACTTAGATGAGAt                                                                                                                                  >  1:498542/1‑149 (MQ=255)
                  aaGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAtt                                                                                                                                 >  2:4424186/1‑149 (MQ=255)
                  aaGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAtt                                                                                                                                 <  1:2806700/149‑1 (MQ=255)
                       aCGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAgg                                                                                                                            >  1:1023674/1‑149 (MQ=255)
                          cggcgTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGaaa                                                                                                                         <  2:2817226/149‑1 (MQ=255)
                           ggcgTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATTAACAGGGGCGGCGTTGCTGCACATGTCGGCTGGGTACGGCAGTTTTTGGATGAACCCAAAACACATAGATGGGATTTCAGGaaaa                                                                                                                        >  1:4303/1‑149 (MQ=255)
                            gcgTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGACTTAGATGAGATTGCAGGAAAAc                                                                                                                       >  2:3895661/1‑149 (MQ=255)
                               tCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCg                                                                                                                    <  1:1020786/149‑1 (MQ=255)
                                 tGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGt                                                                                                                  <  1:2080979/149‑1 (MQ=255)
                                   ccAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTa                                                                                                                <  1:4725880/149‑1 (MQ=255)
                                   ccAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTa                                                                                                                >  2:2177772/1‑149 (MQ=255)
                                     aGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAAATCGGTTAAc                                                                                                              >  1:2575066/1‑149 (MQ=255)
                                         gCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAg                                                                                                          <  1:3102367/149‑1 (MQ=255)
                                         gCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAg                                                                                                          <  2:4458348/149‑1 (MQ=255)
                                          cTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCATCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGt                                                                                                         <  1:509880/149‑1 (MQ=255)
                                           tGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTg                                                                                                        >  2:1560921/1‑149 (MQ=255)
                                           tGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTg                                                                                                        >  2:1600191/1‑149 (MQ=255)
                                            gCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGa                                                                                                       >  1:2721495/1‑149 (MQ=255)
                                            gCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGa                                                                                                       <  1:3636941/149‑1 (MQ=255)
                                            gCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGa                                                                                                       <  1:3953782/149‑1 (MQ=255)
                                            gCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGa                                                                                                       >  2:1669475/1‑149 (MQ=255)
                                                  tGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGt                                                                                                 <  2:27184/149‑1 (MQ=255)
                                                      cGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAAc                                                                                             <  2:1045738/149‑1 (MQ=255)
                                                      cGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAAc                                                                                             <  1:4377148/149‑1 (MQ=255)
                                                          aaaaTCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTg                                                                                         <  2:3781193/149‑1 (MQ=255)
                                                          aaaaTCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTg                                                                                         <  2:3780288/149‑1 (MQ=255)
                                                           aaaTCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgt                                                                                        <  1:2102657/149‑1 (MQ=255)
                                                            aaTCCGAGCTGGGAACTCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGCTGAACCCTACAGACATAGATGAGATTGCAGGACAACTCGGGTAACGGAGTGAGCGAGTTAACATTggt                                                                                       >  2:1736353/1‑147 (MQ=255)
                                                            aaTCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgtt                                                                                       >  1:1988750/1‑149 (MQ=255)
                                                            aaTCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgtt                                                                                       >  2:2306986/1‑149 (MQ=255)
                                                               cAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgttaag                                                                                    <  1:1493459/149‑1 (MQ=255)
                                                                 gagCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTGGCTGCACGTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTgttaagtt                                                                                  <  2:2621455/149‑1 (MQ=255)
                                                                          aaTCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTgg                                                                         <  1:953734/149‑1 (MQ=255)
                                                                              gtcgtTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTc                                                                     <  1:1019428/149‑1 (MQ=255)
                                                                                 gtTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAAc                                                                  <  1:3501105/149‑1 (MQ=255)
                                                                                  ttttCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACt                                                                 >  1:1672568/1‑149 (MQ=255)
                                                                                  ttttCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACt                                                                 >  2:4635722/1‑149 (MQ=255)
                                                                                   tttCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTc                                                                >  1:3373922/1‑149 (MQ=255)
                                                                                    ttCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTcc                                                               <  1:2524791/149‑1 (MQ=255)
                                                                                    ttCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTcc                                                               <  1:3700728/149‑1 (MQ=255)
                                                                                        aTGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGAtt                                                           <  2:4280024/149‑1 (MQ=255)
                                                                                         tGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGAttt                                                          <  1:1930526/149‑1 (MQ=255)
                                                                                           aaCAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTAc                                                        <  2:34398/149‑1 (MQ=255)
                                                                                              aGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATg                                                     >  2:2060872/1‑149 (MQ=255)
                                                                                               ggggCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATgg                                                    <  2:1501773/149‑1 (MQ=255)
                                                                                                gggCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGt                                                   <  2:898006/149‑1 (MQ=255)
                                                                                                gggCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGt                                                   <  1:4740562/149‑1 (MQ=255)
                                                                                                 ggcggcGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGtt                                                  <  1:1141576/149‑1 (MQ=255)
                                                                                                  gcggcgTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTg                                                 <  1:988137/149‑1 (MQ=255)
                                                                                                        ttGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTgtgt                                           <  2:3367839/149‑1 (MQ=255)
                                                                                                            tgcACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTgttgtt                                       >  2:2174076/1‑149 (MQ=255)
                                                                                                            tgcACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTgttgtt                                       <  2:4113137/149‑1 (MQ=255)
                                                                                                              cACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTaa                                     <  1:1339625/149‑1 (MQ=255)
                                                                                                                cgtGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAAt                                   <  2:4237056/147‑1 (MQ=255)
                                                                                                                cTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAAt                                   <  2:2746207/149‑1 (MQ=255)
                                                                                                                cTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAAt                                   <  1:3185663/149‑1 (MQ=255)
                                                                                                                 ttGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAAtt                                  <  2:2049403/149‑1 (MQ=255)
                                                                                                                 ttGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAAtt                                  <  1:83720/149‑1 (MQ=255)
                                                                                                                   gTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGt                                >  2:1796741/1‑149 (MQ=255)
                                                                                                                   gTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGt                                >  2:4186282/1‑149 (MQ=255)
                                                                                                                      ggCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACa                             <  2:876464/149‑1 (MQ=255)
                                                                                                                        cTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACaaa                           >  1:3751975/1‑149 (MQ=255)
                                                                                                                        cTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACaaa                           <  1:693606/149‑1 (MQ=255)
                                                                                                                         tGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAg                          <  2:2128530/149‑1 (MQ=255)
                                                                                                                          gggTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGa                         >  2:1101892/1‑149 (MQ=255)
                                                                                                                          gggTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGa                         <  1:995625/149‑1 (MQ=255)
                                                                                                                           ggTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGAt                        <  2:2922160/149‑1 (MQ=255)
                                                                                                                           ggTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGAt                        <  2:844392/149‑1 (MQ=255)
                                                                                                                             tACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGt                      <  2:4029025/149‑1 (MQ=255)
                                                                                                                              aCGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGtt                     <  1:2983443/149‑1 (MQ=255)
                                                                                                                               cGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTta                    <  1:1020415/149‑1 (MQ=255)
                                                                                                                               cGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTta                    <  1:3231726/149‑1 (MQ=255)
                                                                                                                                ggCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTtat                   >  1:1176241/1‑149 (MQ=255)
                                                                                                                                 gCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTtata                  >  2:863596/1‑149 (MQ=255)
                                                                                                                                 gCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTtata                  <  2:1196347/149‑1 (MQ=255)
                                                                                                                                 gCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTtata                  >  1:4003016/1‑149 (MQ=255)
                                                                                                                                   aGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGTTGTTATAGa                >  2:2476814/1‑149 (MQ=255)
                                                                                                                                    gTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGaa               >  2:4644198/1‑149 (MQ=255)
                                                                                                                                     ttttGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGaaa              <  2:2577717/149‑1 (MQ=255)
                                                                                                                                     ttttGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGaaa              <  2:3515536/149‑1 (MQ=255)
                                                                                                                                          ggATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACaaaa         <  1:3282684/149‑1 (MQ=255)
                                                                                                                                          ggATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACaaaa         <  1:4263269/149‑1 (MQ=255)
                                                                                                                                          ggATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTAGAGAAACaaaa         <  2:1914727/149‑1 (MQ=255)
                                                                                                                                           gATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAAt        <  2:3702551/149‑1 (MQ=255)
                                                                                                                                           gATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGACGTTATAGAAACAAAAt        <  2:200954/149‑1 (MQ=255)
                                                                                                                                              gAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTa     <  1:2557280/149‑1 (MQ=255)
                                                                                                                                              gAAACCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTa     <  2:1866182/149‑1 (MQ=255)
                                                                                                                                                aCCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAAc   <  1:2202833/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CGCCCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACA  >  CP009273/625026‑625319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCT  >  CP009273/625029‑625322
                                                                                                                                                 |                                                                                                                                                    
CCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAG                                                                                                                                                  >  SRR19973071.1575700/1‑150 (MQ=60)
CCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAG                                                                                                                                                  >  SRR19973071.2722414/1‑150 (MQ=60)
      TCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAATATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAG                                                                                                                                            <  SRR19973071.1425250/150‑1 (MQ=60)
      TCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAG                                                                                                                                            <  SRR19973071.677845/150‑1 (MQ=60)
      TCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAG                                                                                                                                            <  SRR19973071.1425790/150‑1 (MQ=60)
         GAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACCGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGTTGTACCCTACAGAATTAGTTG                                                                                                                                         >  SRR19973071.201777/1‑150 (MQ=60)
         GAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATG                                                                                                                                         >  SRR19973071.320553/1‑150 (MQ=60)
         GAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATG                                                                                                                                         <  SRR19973071.1929240/150‑1 (MQ=60)
         GAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATG                                                                                                                                         <  SRR19973071.1929227/150‑1 (MQ=60)
         GAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCAACAGAATTAGATG                                                                                                                                         >  SRR19973071.201842/1‑150 (MQ=60)
          AGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATTAACAGGGGCGGCGTTGCTGCACATGTCGGCTGGGTACGGCAGGTTTGGGATGAACCCTACAGAATTATATGA                                                                                                                                        >  SRR19973071.2888506/1‑150 (MQ=60)
           GGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACACTACAGAATTAGATGAG                                                                                                                                       >  SRR19973071.3554128/1‑150 (MQ=60)
             GAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAT                                                                                                                                     <  SRR19973071.624760/150‑1 (MQ=60)
             GAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGAT                                                                                                                                     <  SRR19973071.655772/150‑1 (MQ=60)
             GAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGGTGCTGCACGTGTCGGCTGGGTACGGCAGTTTTGGGGTGTACCCTACAGACTTAGATGAGAT                                                                                                                                     >  SRR19973071.500393/1‑150 (MQ=60)
               AAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTG                                                                                                                                   <  SRR19973071.2816590/150‑1 (MQ=60)
               AAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTG                                                                                                                                   <  SRR19973071.2349813/150‑1 (MQ=60)
                   TACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGG                                                                                                                               >  SRR19973071.1027380/1‑150 (MQ=60)
                        GGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTAGATTAACAGGGGCGGCGTTGCTGCACATGTCGGCGGGGTACGGCAGTTTTTGGCTGAACCCAAAAGAATTATATTAGATTGCAGGAAAAA                                                                                                                          >  SRR19973071.2804490/1‑150 (MQ=60)
                           GTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCG                                                                                                                       <  SRR19973071.4357142/150‑1 (MQ=60)
                            TCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGG                                                                                                                      <  SRR19973071.1024486/150‑1 (MQ=60)
                              TGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACATAATTAGATGAGATTGCAGGAAAACTCGGTT                                                                                                                    <  SRR19973071.2088668/150‑1 (MQ=60)
                              TGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTT                                                                                                                    <  SRR19973071.2088697/150‑1 (MQ=60)
                              TGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTT                                                                                                                    <  SRR19973071.2088642/150‑1 (MQ=60)
                                CCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTTCGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAA                                                                                                                  <  SRR19973071.1059084/150‑1 (MQ=60)
                                CCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAA                                                                                                                  <  SRR19973071.4742623/150‑1 (MQ=60)
                                 CAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAAATCGGTTAAC                                                                                                                 >  SRR19973071.2584401/1‑150 (MQ=60)
                                      GCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGT                                                                                                            <  SRR19973071.3113303/150‑1 (MQ=60)
                                      GCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGT                                                                                                            <  SRR19973071.3113321/150‑1 (MQ=60)
                                       CTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCATCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTG                                                                                                           <  SRR19973071.510364/150‑1 (MQ=60)
                                       CTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCATCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTG                                                                                                           <  SRR19973071.511783/150‑1 (MQ=60)
                                        TGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTTGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACCGAGTGA                                                                                                          >  SRR19973071.2731178/1‑150 (MQ=60)
                                         GCATCTTGGTCGGCAAAATCAGCGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGAT                                                                                                         <  SRR19973071.3967783/150‑1 (MQ=60)
                                         GCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTTGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGAT                                                                                                         >  SRR19973071.2631317/1‑150 (MQ=60)
                                         GCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGAT                                                                                                         <  SRR19973071.3649727/150‑1 (MQ=60)
                                         GCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGAT                                                                                                         <  SRR19973071.3967662/150‑1 (MQ=60)
                                           ATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCCGTTTTGGGATTAACCCGACAGAATTAGATGAGATTGCAGGAAAACACGGTTAACGGAGTGATCG                                                                                                       >  SRR19973071.875660/1‑150 (MQ=60)
                                           ATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCG                                                                                                       >  SRR19973071.4407077/1‑150 (MQ=60)
                                              TTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCGGGGTACGGCAGTTTTTGGATGAAACCAACAGAATTAGATGAGATTTCAGGAAAAATCGGTTAACGGAGTGATCGAGT                                                                                                    >  SRR19973071.3637272/1‑150 (MQ=60)
                                                GGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTA                                                                                                  <  SRR19973071.3154235/150‑1 (MQ=60)
                                                  TCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAAC                                                                                                <  SRR19973071.626011/150‑1 (MQ=60)
                                                   CGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACA                                                                                               <  SRR19973071.4392524/150‑1 (MQ=60)
                                                    GGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACAT                                                                                              <  SRR19973071.2896783/150‑1 (MQ=60)
                                                       AAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCCCTTGTCGGCTGGGTACGGCAGTTTTGGGGTGAACCCTACAGAATTCGATGTGATTGCAGGCAAACTCGGTTAACGGGGTGATCGAGgtatccttgt                                                                                           >  SRR19973071.1528427/1‑140 (MQ=60)
                                                       AAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGT                                                                                           >  SRR19973071.1528496/1‑150 (MQ=60)
                                                        AAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTT                                                                                          >  SRR19973071.1995930/1‑150 (MQ=60)
                                                        AAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTT                                                                                          <  SRR19973071.2110409/150‑1 (MQ=60)
                                                        AAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTT                                                                                          >  SRR19973071.1995912/1‑150 (MQ=60)
                                                         AATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTTTCGGCTGGGTACGGCAGTTTTTGGATGAACCCAACAGAATTATATGAGATTGCAGGAAAACACGGTTAACCGAGTGATCGAGTTAACATTGTTA                                                                                         >  SRR19973071.1352390/1‑150 (MQ=60)
                                                            CAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGT                                                                                      <  SRR19973071.1498870/150‑1 (MQ=60)
                                                                     GAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGACAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTG                                                                             <  SRR19973071.3327917/150‑1 (MQ=60)
                                                                      AAATCGTCGTTTTCGATGAACAGGGGCGGCGTTTCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGG                                                                            >  SRR19973071.372592/1‑150 (MQ=60)
                                                                      AAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGG                                                                            <  SRR19973071.997363/150‑1 (MQ=60)
                                                                       AATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTTGGATGAACCCTACAGAATTAGATGAGATTTCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGT                                                                           >  SRR19973071.3503948/1‑150 (MQ=60)
                                                                       AATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGT                                                                           <  SRR19973071.957198/150‑1 (MQ=60)
                                                                       AATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATAGAGTTAACATTGTTAAGTTAAATATTGGT                                                                           >  SRR19973071.3888640/1‑150 (MQ=60)
                                                                           GTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCA                                                                       <  SRR19973071.1023124/150‑1 (MQ=60)
                                                                             CGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAAC                                                                     <  SRR19973071.3110807/150‑1 (MQ=60)
                                                                              GTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACT                                                                    >  SRR19973071.1678715/1‑150 (MQ=60)
                                                                              GTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACT                                                                    <  SRR19973071.3513399/150‑1 (MQ=60)
                                                                              GTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACT                                                                    >  SRR19973071.2161846/1‑150 (MQ=60)
                                                                               TTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTC                                                                   >  SRR19973071.3385894/1‑150 (MQ=60)
                                                                               TTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTC                                                                   >  SRR19973071.3385868/1‑150 (MQ=60)
                                                                                 TTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCG                                                                 <  SRR19973071.2533980/150‑1 (MQ=60)
                                                                                 TTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCG                                                                 <  SRR19973071.3713834/150‑1 (MQ=60)
                                                                                  TCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGA                                                                >  SRR19973071.1136441/1‑150 (MQ=60)
                                                                                     ATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTT                                                             <  SRR19973071.4525643/150‑1 (MQ=60)
                                                                                     ATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTT                                                             <  SRR19973071.4752890/150‑1 (MQ=60)
                                                                                     ATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTT                                                             >  SRR19973071.4770740/1‑150 (MQ=60)
                                                                                     ATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTT                                                             >  SRR19973071.3914399/1‑150 (MQ=60)
                                                                                     ATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTT                                                             <  SRR19973071.1457004/150‑1 (MQ=60)
                                                                                      TGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTA                                                            <  SRR19973071.1937564/150‑1 (MQ=60)
                                                                                      TGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTA                                                            <  SRR19973071.1937825/150‑1 (MQ=60)
                                                                                      TGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTA                                                            >  SRR19973071.2543181/1‑150 (MQ=60)
                                                                                           AGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGG                                                       >  SRR19973071.581051/1‑150 (MQ=60)
                                                                                           AGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGG                                                       >  SRR19973071.962637/1‑150 (MQ=60)
                                                                                             GGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTT                                                     <  SRR19973071.4757345/150‑1 (MQ=60)
                                                                                              GGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTG                                                    <  SRR19973071.1145562/150‑1 (MQ=60)
                                                                                              GGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTG                                                    <  SRR19973071.1145573/150‑1 (MQ=60)
                                                                                               GCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGC                                                   <  SRR19973071.991730/150‑1 (MQ=60)
                                                                                                CGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCT                                                  >  SRR19973071.675046/1‑150 (MQ=60)
                                                                                                CGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCT                                                  <  SRR19973071.3757613/150‑1 (MQ=60)
                                                                                                      TGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTG                                            >  SRR19973071.1026264/1‑150 (MQ=60)
                                                                                                       GCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGT                                           <  SRR19973071.192209/150‑1 (MQ=60)
                                                                                                       GCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGT                                           <  SRR19973071.192184/150‑1 (MQ=60)
                                                                                                       GCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGT                                           >  SRR19973071.2009788/1‑150 (MQ=60)
                                                                                                           CACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAA                                       <  SRR19973071.1344527/150‑1 (MQ=60)
                                                                                                           CACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAA                                       <  SRR19973071.1344550/150‑1 (MQ=60)
                                                                                                           CACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAA                                       <  SRR19973071.4251779/150‑1 (MQ=60)
                                                                                                             CTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATT                                     <  SRR19973071.3196747/150‑1 (MQ=60)
                                                                                                             CTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATT                                     <  SRR19973071.3197347/150‑1 (MQ=60)
                                                                                                              TTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTG                                    <  SRR19973071.84162/150‑1 (MQ=60)
                                                                                                                   GGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAA                               >  SRR19973071.2339851/1‑150 (MQ=60)
                                                                                                                   GGCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAA                               >  SRR19973071.2563977/1‑150 (MQ=60)
                                                                                                                    GCTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAA                              >  SRR19973071.3765290/1‑150 (MQ=60)
                                                                                                                     CTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAG                             <  SRR19973071.696194/150‑1 (MQ=60)
                                                                                                                     CTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAG                             <  SRR19973071.696160/150‑1 (MQ=60)
                                                                                                                     CTGGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAG                             <  SRR19973071.696065/150‑1 (MQ=60)
                                                                                                                       GGGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGAT                           <  SRR19973071.999248/150‑1 (MQ=60)
                                                                                                                        GGTACGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATG                          >  SRR19973071.4168170/1‑150 (MQ=60)
                                                                                                                           ACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTA                       <  SRR19973071.2994119/150‑1 (MQ=60)
                                                                                                                           ACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTA                       <  SRR19973071.594555/150‑1 (MQ=60)
                                                                                                                            CGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTAT                      <  SRR19973071.1024110/150‑1 (MQ=60)
                                                                                                                            CGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTAT                      >  SRR19973071.1180292/1‑150 (MQ=60)
                                                                                                                            CGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTAT                      <  SRR19973071.1024274/150‑1 (MQ=60)
                                                                                                                            CGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTAT                      <  SRR19973071.1024271/150‑1 (MQ=60)
                                                                                                                            CGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTAT                      <  SRR19973071.1024115/150‑1 (MQ=60)
                                                                                                                            CGGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTAT                      <  SRR19973071.3243096/150‑1 (MQ=60)
                                                                                                                             GGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATA                     >  SRR19973071.4017032/1‑150 (MQ=60)
                                                                                                                             GGCAGTTTTGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATA                     <  SRR19973071.1196165/150‑1 (MQ=60)
                                                                                                                                    TTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAA              <  SRR19973071.3682679/150‑1 (MQ=60)
                                                                                                                                     TGGGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAA             <  SRR19973071.959385/150‑1 (MQ=60)
                                                                                                                                     TGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAA             >  SRR19973071.4799332/1‑150 (MQ=60)
                                                                                                                                       GGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAAT           <  SRR19973071.3293993/150‑1 (MQ=60)
                                                                                                                                       GGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAAT           <  SRR19973071.3294313/150‑1 (MQ=60)
                                                                                                                                       GGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAAT           <  SRR19973071.4278385/150‑1 (MQ=60)
                                                                                                                                       GGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAAT           <  SRR19973071.4278396/150‑1 (MQ=60)
                                                                                                                                       GGATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAAATCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAAT           <  SRR19973071.1675606/150‑1 (MQ=60)
                                                                                                                                        GATGAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATG          <  SRR19973071.4323818/150‑1 (MQ=60)
                                                                                                                                           GAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAA       <  SRR19973071.2566921/150‑1 (MQ=60)
                                                                                                                                           GAACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAA       <  SRR19973071.2566565/150‑1 (MQ=60)
                                                                                                                                             ACCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACA     <  SRR19973071.2210984/150‑1 (MQ=60)
                                                                                                                                              CCCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACAT    <  SRR19973071.2068446/150‑1 (MQ=60)
                                                                                                                                               CCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATC   >  SRR19973071.138685/1‑150 (MQ=60)
                                                                                                                                               CCTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATC   >  SRR19973071.1926737/1‑150 (MQ=60)
                                                                                                                                                CTACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCT  <  SRR19973071.4289263/150‑1 (MQ=60)
                                                                                                                                                 |                                                                                                                                                    
CCGGTGTCTGAGGGAAAGGTACGCGGCGTCTGCCAGCCGCTGCATCTTGGTCGGCAAAATCAGAGCTGGGAAATCGTCGTTTTCGATGAACAGGGGCGGCGTTGCTGCACTTGTCGGCTGGGTACGGCAGTTTTGGGATGAACCCGACAGAATTAGATGAGATTGCAGGAAAACTCGGTTAACGGAGTGATCGAGTTAACATTGTTAAGTTAAATATTGGTTTCAACTCCGATTTACATGGTTGCTGTGTTGTTAAATTGTACAAAGATGTTATAGAAACAAAATGTAACATCT  >  CP009273/625029‑625322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑