Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,049,220 C→A 93.0% R84R (CGG→AGG)  zapA → FtsZ stabilizer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,049,2200CA93.0% 525.6 / 30.7 187R84R (CGG→AGG) zapAFtsZ stabilizer
Reads supporting (aligned to +/- strand):  ref base C (5/8);  new base A (85/89);  total (90/97)
Fisher's exact test for biased strand distribution p-value = 5.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 187 total aligned reads displayed.

CCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGC  >  CP009273/3049075‑3049364
                                                                                                                                                 |                                                                                                                                                
ccAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAgg                                                                                                                                                <  2:895857/148‑1 (MQ=255)
    cGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGAtgc                                                                                                                                            <  2:2226090/148‑1 (MQ=255)
       ttGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCTTATTAGGAtgctgc                                                                                                                                         <  2:1065757/148‑1 (MQ=255)
        tGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTgca                                                                                                                                        <  2:1989269/148‑1 (MQ=255)
              aTCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGAc                                                                                                                                  <  1:3439321/148‑1 (MQ=255)
                     aGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGaa                                                                                                                           >  1:467722/1‑148 (MQ=255)
                     aGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGaa                                                                                                                           >  2:2996415/1‑148 (MQ=255)
                       aaCGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACa                                                                                                                         <  1:1849877/148‑1 (MQ=255)
                         cGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAg                                                                                                                       >  1:892506/1‑148 (MQ=255)
                           cACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCg                                                                                                                     <  2:2362077/148‑1 (MQ=255)
                            aCTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGt                                                                                                                    <  2:56738/148‑1 (MQ=255)
                              tAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTa                                                                                                                  >  2:946069/1‑148 (MQ=255)
                              tAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTa                                                                                                                  <  1:2006498/148‑1 (MQ=255)
                               agagTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTAc                                                                                                                 >  2:3387879/1‑148 (MQ=255)
                               agagTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTAc                                                                                                                 <  1:3009864/148‑1 (MQ=255)
                                gagTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACt                                                                                                                <  2:1356903/148‑1 (MQ=255)
                                gagTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACt                                                                                                                >  2:1287697/1‑148 (MQ=255)
                                   tCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGa                                                                                                             >  2:2208318/1‑148 (MQ=255)
                                    cacaAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGTAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGaa                                                                                                            >  1:1308273/1‑148 (MQ=255)
                                    cacaAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGaa                                                                                                            >  1:1882342/1‑148 (MQ=255)
                                     acaAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTCGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAAc                                                                                                           >  1:1763962/1‑148 (MQ=255)
                                      caAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACa                                                                                                          <  2:320742/148‑1 (MQ=255)
                                       aaaTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACaa                                                                                                         >  2:1784299/1‑148 (MQ=255)
                                        aaTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAg                                                                                                        >  2:120750/1‑148 (MQ=255)
                                         aTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAgg                                                                                                       <  1:3309160/148‑1 (MQ=255)
                                         aTACTGAACAGTTGGTCTTCATTGCCGCATAGAATATCAGCTATGAGTTAGCGCAATAAAAAGCAAAGACTCGTGAATACGCGGCAATTATGGAACAGAGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAAAAAgg                                                                                                       >  1:2289502/1‑148 (MQ=255)
                                           aCTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTc                                                                                                     >  1:2280823/1‑148 (MQ=255)
                                           aCTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTc                                                                                                     >  1:1880909/1‑148 (MQ=255)
                                           aCTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTc                                                                                                     >  1:492712/1‑148 (MQ=255)
                                           aCTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTc                                                                                                     >  1:3092613/1‑148 (MQ=255)
                                           aCTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTc                                                                                                     >  1:3089469/1‑148 (MQ=255)
                                               aaCAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCAt                                                                                                 >  2:2159141/1‑148 (MQ=255)
                                                   gTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCAcc                                                                                             >  1:2542073/1‑148 (MQ=255)
                                                    ttGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCg                                                                                            >  1:1456539/1‑148 (MQ=255)
                                                        tCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGaaaa                                                                                        >  1:2904888/1‑148 (MQ=255)
                                                         cTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGaaaaa                                                                                       >  2:2358031/1‑148 (MQ=255)
                                                          ttCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGaaaaaa                                                                                      <  2:2756132/148‑1 (MQ=255)
                                                               tGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAAc                                                                                 <  1:258607/148‑1 (MQ=255)
                                                                  cGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCaa                                                                              <  1:861276/148‑1 (MQ=255)
                                                                   gCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCaaa                                                                             <  2:2521039/148‑1 (MQ=255)
                                                                     aTTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAAc                                                                           <  2:42347/148‑1 (MQ=255)
                                                                     aTTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAAc                                                                           <  2:105421/148‑1 (MQ=255)
                                                                      ttGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACt                                                                          >  2:632860/1‑148 (MQ=255)
                                                                       tGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACtt                                                                         >  1:2456653/1‑148 (MQ=255)
                                                                       tGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACtt                                                                         >  1:1594811/1‑148 (MQ=255)
                                                                         aaTATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTg                                                                       >  2:2395091/1‑148 (MQ=255)
                                                                         aaTATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTg                                                                       >  2:2966959/1‑148 (MQ=255)
                                                                          atatCAGCTCTGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGa                                                                      >  1:3046612/1‑148 (MQ=255)
                                                                          atatCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGa                                                                      >  1:1586624/1‑148 (MQ=255)
                                                                          atatCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGa                                                                      >  1:3046620/1‑148 (MQ=255)
                                                                           tatCAGCTATGAGTTAGCTCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCTTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGaa                                                                     <  2:2861049/148‑1 (MQ=255)
                                                                           tatCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGaa                                                                     >  2:1470221/1‑148 (MQ=255)
                                                                            atCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAAt                                                                    >  1:3415306/1‑148 (MQ=255)
                                                                             tCAGCTATGAGTTAGCTCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATg                                                                   >  1:1187312/1‑148 (MQ=255)
                                                                             tCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATg                                                                   <  2:3130002/148‑1 (MQ=255)
                                                                             tCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATg                                                                   <  1:1035725/148‑1 (MQ=255)
                                                                             tCAGATATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACACGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATg                                                                   <  1:3003943/148‑1 (MQ=255)
                                                                                gCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGaca                                                                <  2:2324314/148‑1 (MQ=255)
                                                                                gCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGaca                                                                <  1:1557676/148‑1 (MQ=255)
                                                                                  tATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACt                                                              <  2:2310604/148‑1 (MQ=255)
                                                                                    tGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACttt                                                            >  2:2394830/1‑148 (MQ=255)
                                                                                     gAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACtttt                                                           <  1:2852438/148‑1 (MQ=255)
                                                                                     gAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACtttt                                                           <  1:1077362/148‑1 (MQ=255)
                                                                                     gAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACtttt                                                           <  1:2597067/148‑1 (MQ=255)
                                                                                          aGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGtt                                                      <  2:2448084/148‑1 (MQ=255)
                                                                                            cgcAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTa                                                    <  2:855297/148‑1 (MQ=255)
                                                                                            cgcAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTa                                                    <  2:776098/148‑1 (MQ=255)
                                                                                            cgcAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTa                                                    <  2:774545/148‑1 (MQ=255)
                                                                                                aGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGTAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACtgt                                                <  2:44429/148‑1 (MQ=255)
                                                                                                 gAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACtgtg                                               <  2:2756963/148‑1 (MQ=255)
                                                                                                      aGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTaga                                          <  1:3441063/148‑1 (MQ=255)
                                                                                                      aGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTaga                                          <  2:1587696/148‑1 (MQ=255)
                                                                                                       gCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTagag                                         <  1:3293337/148‑1 (MQ=255)
                                                                                                        cAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGt                                        >  2:1402249/1‑148 (MQ=255)
                                                                                                        cAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGt                                        >  2:3051110/1‑148 (MQ=255)
                                                                                                          aaGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGtca                                      >  1:672913/1‑146 (MQ=255)
                                                                                                          aaGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTaa                                      <  1:2212405/148‑1 (MQ=255)
                                                                                                          aaGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTaa                                      >  1:3321628/1‑148 (MQ=255)
                                                                                                           aGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAAc                                     >  2:1428258/1‑148 (MQ=255)
                                                                                                            gACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAAcc                                    >  1:2905928/1‑148 (MQ=255)
                                                                                                             aCTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGTTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCg                                   >  1:49944/1‑148 (MQ=255)
                                                                                                               tCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTg                                 <  2:2745423/148‑1 (MQ=255)
                                                                                                                  tGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAg                              <  1:2068102/148‑1 (MQ=255)
                                                                                                                  tGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAg                              >  1:1924619/1‑148 (MQ=255)
                                                                                                                  tGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAg                              <  2:1037275/148‑1 (MQ=255)
                                                                                                                   gACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGa                             <  1:940548/148‑1 (MQ=255)
                                                                                                                    aCTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGAc                            >  2:176202/1‑148 (MQ=255)
                                                                                                                        cgcgGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACaaaa                        >  2:2100726/1‑148 (MQ=255)
                                                                                                                        cgcgGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACaaaa                        <  2:75439/148‑1 (MQ=255)
                                                                                                                        cgcgGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACaaaa                        <  2:75669/148‑1 (MQ=255)
                                                                                                                              aaGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTtctc                  <  2:3464773/148‑1 (MQ=255)
                                                                                                                              aaGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTtctc                  <  2:1704762/148‑1 (MQ=255)
                                                                                                                                    ggAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGAt            >  2:2177746/1‑148 (MQ=255)
                                                                                                                                     gAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATg           >  2:354129/1‑148 (MQ=255)
                                                                                                                                     gAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATg           <  2:1625712/148‑1 (MQ=255)
                                                                                                                                     gAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATg           <  2:812961/148‑1 (MQ=255)
                                                                                                                                      aaCAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGt          <  2:2904957/148‑1 (MQ=255)
                                                                                                                                         aGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCg       <  1:1788226/148‑1 (MQ=255)
                                                                                                                                          gCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGc      >  1:1148134/1‑148 (MQ=255)
                                                                                                                                             tATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAg   >  1:1031845/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGC  >  CP009273/3049075‑3049364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGAACCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGC  >  CP009273/3049071‑3049368
                                                                                                                                                     |                                                                                                                                                    
TGAACCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTA                                                                                                                                                      <  SRR19973067.168811/150‑1 (MQ=60)
 GAACCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAG                                                                                                                                                     >  SRR19973067.106156/1‑150 (MQ=60)
   ACCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGA                                                                                                                                                   >  SRR19973067.901218/1‑150 (MQ=60)
               AAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGA                                                                                                                                       >  SRR19973067.1096022/1‑150 (MQ=60)
                  ATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCA                                                                                                                                    <  SRR19973067.3461219/150‑1 (MQ=60)
                       AAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAA                                                                                                                               >  SRR19973067.470957/1‑150 (MQ=60)
                           AACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAG                                                                                                                           >  SRR19973067.898408/1‑150 (MQ=60)
                           AACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAG                                                                                                                           <  SRR19973067.1862488/150‑1 (MQ=60)
                                 CTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTAC                                                                                                                     <  SRR19973067.653969/150‑1 (MQ=60)
                                 CTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTAC                                                                                                                     <  SRR19973067.1369988/150‑1 (MQ=60)
                                  TAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACT                                                                                                                    <  SRR19973067.2020010/150‑1 (MQ=60)
                                   AGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTT                                                                                                                   <  SRR19973067.3029008/150‑1 (MQ=60)
                                      GTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGTAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAA                                                                                                                >  SRR19973067.1317198/1‑150 (MQ=60)
                                      GTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAA                                                                                                                >  SRR19973067.1895188/1‑150 (MQ=60)
                                       TCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTCGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAAC                                                                                                               >  SRR19973067.1775961/1‑150 (MQ=60)
                                       TCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAAC                                                                                                               >  SRR19973067.1776807/1‑150 (MQ=60)
                                         ACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAA                                                                                                             <  SRR19973067.3052831/150‑1 (MQ=60)
                                           AAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGG                                                                                                           >  SRR19973067.2302966/1‑150 (MQ=60)
                                           AAATACTGAACAGTTGGTCTTCATTGCCGCATAGAATATCAGCTATGAGTTAGCGCAATAAAAAGCAAAGACTCGTGAATACGCGGCAATTATGGAACAGAGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAAAAAGG                                                                                                           >  SRR19973067.2304630/1‑150 (MQ=60)
                                             ATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTC                                                                                                         <  SRR19973067.223488/150‑1 (MQ=60)
                                             ATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTC                                                                                                         >  SRR19973067.2295905/1‑150 (MQ=60)
                                             ATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTC                                                                                                         >  SRR19973067.1893743/1‑150 (MQ=60)
                                             ATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTC                                                                                                         >  SRR19973067.3109127/1‑150 (MQ=60)
                                             ATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTC                                                                                                         >  SRR19973067.3112284/1‑150 (MQ=60)
                                             ATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTC                                                                                                         <  SRR19973067.3330049/150‑1 (MQ=60)
                                             ATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTC                                                                                                         >  SRR19973067.496100/1‑150 (MQ=60)
                                             ATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTC                                                                                                         >  SRR19973067.496930/1‑150 (MQ=60)
                                              TACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCG                                                                                                        >  SRR19973067.1044413/1‑150 (MQ=60)
                                               ACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGC                                                                                                       >  SRR19973067.2774779/1‑150 (MQ=60)
                                                   AACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCA                                                                                                   >  SRR19973067.1911911/1‑150 (MQ=60)
                                                     CAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACC                                                                                                 >  SRR19973067.2558715/1‑150 (MQ=60)
                                                     CAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACC                                                                                                 >  SRR19973067.2558648/1‑150 (MQ=60)
                                                      AGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCG                                                                                                >  SRR19973067.1466289/1‑150 (MQ=60)
                                                          GGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAA                                                                                            >  SRR19973067.2923473/1‑150 (MQ=60)
                                                          GGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAA                                                                                            >  SRR19973067.2923404/1‑150 (MQ=60)
                                                              TTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACT                                                                                        <  SRR19973067.2364778/150‑1 (MQ=60)
                                                                   TGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCA                                                                                   <  SRR19973067.2902209/150‑1 (MQ=60)
                                                                   TGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCA                                                                                   <  SRR19973067.260377/150‑1 (MQ=60)
                                                                      CGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAA                                                                                <  SRR19973067.867057/150‑1 (MQ=60)
                                                                         ATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTT                                                                             >  SRR19973067.1599406/1‑150 (MQ=60)
                                                                         ATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTT                                                                             >  SRR19973067.1605748/1‑150 (MQ=60)
                                                                         ATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTT                                                                             <  SRR19973067.1550144/150‑1 (MQ=60)
                                                                         ATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTT                                                                             >  SRR19973067.2472806/1‑150 (MQ=60)
                                                                          TTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTT                                                                            <  SRR19973067.3101596/150‑1 (MQ=60)
                                                                           TGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGACAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTG                                                                           <  SRR19973067.3317465/150‑1 (MQ=60)
                                                                            GAATATCAGCTCTGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGA                                                                          >  SRR19973067.3066020/1‑150 (MQ=60)
                                                                            GAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGA                                                                          >  SRR19973067.1597490/1‑150 (MQ=60)
                                                                            GAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGA                                                                          >  SRR19973067.3066028/1‑150 (MQ=60)
                                                                            GAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGA                                                                          <  SRR19973067.3440961/150‑1 (MQ=60)
                                                                             AATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAA                                                                         >  SRR19973067.1889986/1‑150 (MQ=60)
                                                                              ATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAAT                                                                        >  SRR19973067.3437050/1‑150 (MQ=60)
                                                                               TATCAGCTATGAGTTAGCTCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATG                                                                       >  SRR19973067.1195131/1‑150 (MQ=60)
                                                                                 TCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGAC                                                                     <  SRR19973067.3023071/150‑1 (MQ=60)
                                                                                 TCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGAC                                                                     <  SRR19973067.3022165/150‑1 (MQ=60)
                                                                                 TCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGAC                                                                     <  SRR19973067.1042850/150‑1 (MQ=60)
                                                                                 TCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGAC                                                                     <  SRR19973067.475461/150‑1 (MQ=60)
                                                                                 TCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGAC                                                                     <  SRR19973067.119619/150‑1 (MQ=60)
                                                                                 TCAGATATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACACGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGAC                                                                     <  SRR19973067.3023016/150‑1 (MQ=60)
                                                                                   AGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACAC                                                                   >  SRR19973067.3339080/1‑150 (MQ=60)
                                                                                    GCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACT                                                                  <  SRR19973067.1568295/150‑1 (MQ=60)
                                                                                    GCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACT                                                                  <  SRR19973067.3110098/150‑1 (MQ=60)
                                                                                        TGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTC                                                              <  SRR19973067.1829156/150‑1 (MQ=60)
                                                                                         GAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCG                                                             <  SRR19973067.2870735/150‑1 (MQ=60)
                                                                                         GAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCG                                                             <  SRR19973067.1084744/150‑1 (MQ=60)
                                                                                         GAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCG                                                             <  SRR19973067.2613959/150‑1 (MQ=60)
                                                                                              AGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTA                                                        <  SRR19973067.1727795/150‑1 (MQ=60)
                                                                                                          AGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGT                                            <  SRR19973067.3462964/150‑1 (MQ=60)
                                                                                                           GCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTA                                           <  SRR19973067.3313957/150‑1 (MQ=60)
                                                                                                           GCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTA                                           <  SRR19973067.3314051/150‑1 (MQ=60)
                                                                                                            CAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTCA                                          >  SRR19973067.677525/1‑150 (MQ=60)
                                                                                                            CAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAA                                          >  SRR19973067.677563/1‑150 (MQ=60)
                                                                                                            CAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAA                                          >  SRR19973067.3342639/1‑150 (MQ=60)
                                                                                                             AAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAAC                                         <  SRR19973067.445463/150‑1 (MQ=60)
                                                                                                              AAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACC                                        >  SRR19973067.2924448/1‑150 (MQ=60)
                                                                                                              AAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACC                                        <  SRR19973067.2227146/150‑1 (MQ=60)
                                                                                                               AGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGTTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCG                                       >  SRR19973067.50599/1‑150 (MQ=60)
                                                                                                                GACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGT                                      <  SRR19973067.3409412/150‑1 (MQ=60)
                                                                                                                GACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGT                                      <  SRR19973067.258735/150‑1 (MQ=60)
                                                                                                                  CTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGA                                    >  SRR19973067.2083246/1‑150 (MQ=60)
                                                                                                                    CGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAG                                  <  SRR19973067.886695/150‑1 (MQ=60)
                                                                                                                    CGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAG                                  >  SRR19973067.1937751/1‑150 (MQ=60)
                                                                                                                      TGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGAC                                <  SRR19973067.2080463/150‑1 (MQ=60)
                                                                                                                      TGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGAC                                <  SRR19973067.2081849/150‑1 (MQ=60)
                                                                                                                       GACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACA                               <  SRR19973067.946955/150‑1 (MQ=60)
                                                                                                                                          AACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTC            <  SRR19973067.1297161/150‑1 (MQ=60)
                                                                                                                                            CAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGC          >  SRR19973067.1155826/1‑150 (MQ=60)
                                                                                                                                             AGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCA         <  SRR19973067.1800281/150‑1 (MQ=60)
                                                                                                                                               CGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAG       >  SRR19973067.1894105/1‑150 (MQ=60)
                                                                                                                                               CGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAG       >  SRR19973067.1038948/1‑150 (MQ=60)
                                                                                                                                                  ATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGATTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGG    >  SRR19973067.101237/1‑150 (MQ=60)
                                                                                                                                                    TAGGATGCTGCAGCAGACCATATAAAAAGCGTTACTTGAAAAAGGTCGCATCACAGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTAATGTGGTAGATTAACCGTGAAGACAAAAATTCTCTGAGATGTTCGCAAGCGTGC  >  SRR19973067.1828399/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TGAACCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGC  >  CP009273/3049071‑3049368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑