Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,049,220 C→A 100% R84R (CGG→AGG)  zapA → FtsZ stabilizer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,049,2200CA96.1% 692.1 / 14.6 228R84R (CGG→AGG) zapAFtsZ stabilizer
Reads supporting (aligned to +/- strand):  ref base C (5/4);  new base A (104/115);  total (109/119)
Fisher's exact test for biased strand distribution p-value = 7.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 228 total aligned reads displayed.

CCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCA  >  CP009273/3049075‑3049361
                                                                                                                                                 |                                                                                                                                             
ccAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAgg                                                                                                                                             <  2:1074558/148‑1 (MQ=255)
ccAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAgg                                                                                                                                             <  2:2131751/148‑1 (MQ=255)
   aCGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGAtg                                                                                                                                          <  2:2263415/148‑1 (MQ=255)
    cGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGAtgc                                                                                                                                         <  2:2066412/148‑1 (MQ=255)
            aGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGcagcag                                                                                                                                 >  1:1040772/1‑148 (MQ=255)
              aTCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGAc                                                                                                                               <  1:671825/148‑1 (MQ=255)
                   aaaGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGAAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAg                                                                                                                          >  2:2501966/1‑148 (MQ=255)
                    aaGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGa                                                                                                                         >  1:2776799/1‑148 (MQ=255)
                        aCGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACaa                                                                                                                     <  1:419981/148‑1 (MQ=255)
                         cGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAg                                                                                                                    <  1:2301272/148‑1 (MQ=255)
                         cGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAg                                                                                                                    >  1:708579/1‑148 (MQ=255)
                         cGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAg                                                                                                                    >  2:1266105/1‑148 (MQ=255)
                           cACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCg                                                                                                                  <  2:1337545/148‑1 (MQ=255)
                             cTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGtt                                                                                                                >  2:3364175/1‑148 (MQ=255)
                              tAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTa                                                                                                               >  1:2989827/1‑148 (MQ=255)
                               agagTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTAc                                                                                                              >  1:3448249/1‑148 (MQ=255)
                                gagTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACt                                                                                                             <  1:164643/148‑1 (MQ=255)
                                 agTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATNGAATATCAGCTATGAGTTAGCGCANGANAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTANGATGCTGCAGCAGACCATAGAACAAGCGTTACtt                                                                                                            >  1:2389789/1‑148 (MQ=255)
                                   tCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGa                                                                                                          <  2:1979182/148‑1 (MQ=255)
                                    tacaAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTCGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGaa                                                                                                         >  2:3026458/2‑148 (MQ=255)
                                    cacaAATACTGAACAGTTGGTCTTCATTGCCGCATAGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGaa                                                                                                         <  2:513106/148‑1 (MQ=255)
                                       aaaTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACaa                                                                                                      <  2:1680395/148‑1 (MQ=255)
                                        aaTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAg                                                                                                     >  2:345105/1‑148 (MQ=255)
                                         aTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAgg                                                                                                    <  1:3548672/148‑1 (MQ=255)
                                         aTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAgg                                                                                                    <  1:2360326/148‑1 (MQ=255)
                                         aTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAgg                                                                                                    <  1:2378216/148‑1 (MQ=255)
                                         aTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAgg                                                                                                    >  2:1410051/1‑148 (MQ=255)
                                          tACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGt                                                                                                   <  1:2771004/148‑1 (MQ=255)
                                           aCTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTc                                                                                                  <  1:1216731/148‑1 (MQ=255)
                                             tGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGc                                                                                                <  1:1262887/148‑1 (MQ=255)
                                               aaCAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCAt                                                                                              >  1:30221/1‑148 (MQ=255)
                                                aCAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATc                                                                                             >  2:2341998/1‑148 (MQ=255)
                                                 cAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCa                                                                                            >  2:3066995/1‑148 (MQ=255)
                                                  aGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCAc                                                                                           >  2:1084093/1‑148 (MQ=255)
                                                          ttCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGaaaaaa                                                                                   <  1:1951205/148‑1 (MQ=255)
                                                           tCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAAc                                                                                  <  2:378278/148‑1 (MQ=255)
                                                              ttGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTaa                                                                               >  2:65740/1‑148 (MQ=255)
                                                               tGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAACAAACTAAc                                                                              >  2:684068/1‑148 (MQ=255)
                                                               tGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAAc                                                                              <  2:2705016/148‑1 (MQ=255)
                                                                 ccGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCa                                                                            >  1:2862203/1‑148 (MQ=255)
                                                                 ccGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCa                                                                            >  1:1840172/1‑148 (MQ=255)
                                                                   gCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCaaa                                                                          <  1:824785/148‑1 (MQ=255)
                                                                   gCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCaaa                                                                          >  2:1402621/1‑148 (MQ=255)
                                                                   gCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCaaa                                                                          >  2:1226968/1‑148 (MQ=255)
                                                                      ttGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACt                                                                       <  1:928667/148‑1 (MQ=255)
                                                                      ttGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACt                                                                       >  2:1260266/1‑148 (MQ=255)
                                                                      ttGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACt                                                                       >  1:1454295/1‑148 (MQ=255)
                                                                         aaTATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCaa                                                                           >  1:1758543/1‑141 (MQ=255)
                                                                         aaTATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCaa                                                                           <  2:1758543/141‑1 (MQ=255)
                                                                          atatCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGa                                                                   <  1:822973/148‑1 (MQ=255)
                                                                          atatCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGa                                                                   <  1:2609491/148‑1 (MQ=255)
                                                                            atCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAAt                                                                 <  1:3562499/148‑1 (MQ=255)
                                                                            atCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAAt                                                                 >  1:323531/1‑148 (MQ=255)
                                                                             tCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATg                                                                <  1:1111649/148‑1 (MQ=255)
                                                                             tCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATg                                                                <  1:1942468/148‑1 (MQ=255)
                                                                                gCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGaca                                                             <  2:3090756/148‑1 (MQ=255)
                                                                                   aTGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACtt                                                          <  1:1157272/148‑1 (MQ=255)
                                                                                     gAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACtttt                                                        >  2:1888378/1‑148 (MQ=255)
                                                                                        ttAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCgg                                                     <  2:184303/148‑1 (MQ=255)
                                                                                         tAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGt                                                    <  2:1650920/148‑1 (MQ=255)
                                                                                           gcgcAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGttt                                                  >  2:2386805/1‑148 (MQ=255)
                                                                                           gcgcAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGttt                                                  >  1:636387/1‑148 (MQ=255)
                                                                                           gcgcAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGttt                                                  <  1:218028/148‑1 (MQ=255)
                                                                                           gcgcAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGttt                                                  >  1:192074/1‑148 (MQ=255)
                                                                                            cgcAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTa                                                 >  2:1839525/1‑148 (MQ=255)
                                                                                             gcAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTAc                                                <  2:2470396/148‑1 (MQ=255)
                                                                                             gcAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTAc                                                <  2:541228/148‑1 (MQ=255)
                                                                                                  aaaaaGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTgg                                           <  2:2518661/148‑1 (MQ=255)
                                                                                                  aaaaaGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTgg                                           >  1:223707/1‑148 (MQ=255)
                                                                                                     aaGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTag                                        >  2:1694080/1‑148 (MQ=255)
                                                                                                      aGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTaga                                       >  1:348964/1‑148 (MQ=255)
                                                                                                      aGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTaga                                       <  1:35377/148‑1 (MQ=255)
                                                                                                        cAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGt                                     >  2:2945083/1‑148 (MQ=255)
                                                                                                         aaaGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTa                                    <  1:1598471/148‑1 (MQ=255)
                                                                                                         aaaGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTa                                    <  2:2549018/148‑1 (MQ=255)
                                                                                                          aaGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTaa                                   <  2:1185928/148‑1 (MQ=255)
                                                                                                          aaGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTaa                                   <  1:3286575/148‑1 (MQ=255)
                                                                                                           aGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAAc                                  <  1:2279707/148‑1 (MQ=255)
                                                                                                              cTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGt                               <  1:2529395/148‑1 (MQ=255)
                                                                                                               tCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTg                              <  2:2776799/148‑1 (MQ=255)
                                                                                                                       aCGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACaaa                      <  1:2499823/148‑1 (MQ=255)
                                                                                                                          cgGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAAtt                   >  2:834646/1‑148 (MQ=255)
                                                                                                                            gCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTtc                 <  1:2960046/148‑1 (MQ=255)
                                                                                                                                gTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTg             <  2:204066/148‑1 (MQ=255)
                                                                                                                                gTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTg             <  1:455783/148‑1 (MQ=255)
                                                                                                                                 tATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTga            >  1:3118188/1‑148 (MQ=255)
                                                                                                                                 tATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTga            >  1:730358/1‑148 (MQ=255)
                                                                                                                                  aTGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTgag           >  1:891209/1‑148 (MQ=255)
                                                                                                                                  aTGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTgag           <  1:1197347/148‑1 (MQ=255)
                                                                                                                                  aTGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTgag           <  1:1410051/148‑1 (MQ=255)
                                                                                                                                   tGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTgaga          >  1:3165726/1‑148 (MQ=255)
                                                                                                                                   tGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCATTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTgaga          >  2:2673552/1‑148 (MQ=255)
                                                                                                                                     gAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATg        >  1:3139149/1‑148 (MQ=255)
                                                                                                                                     gAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATg        <  1:3094381/148‑1 (MQ=255)
                                                                                                                                       aCAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGtt      >  2:3544372/1‑148 (MQ=255)
                                                                                                                                       aCAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGtt      <  1:2441709/148‑1 (MQ=255)
                                                                                                                                        cAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTc     >  2:1811727/1‑148 (MQ=255)
                                                                                                                                         aGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCg    <  1:589437/148‑1 (MQ=255)
                                                                                                                                           cGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCa  >  2:214637/1‑148 (MQ=255)
                                                                                                                                           cGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTAATGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCa  <  2:3208411/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
CCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCA  >  CP009273/3049075‑3049361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGAACCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCC  >  CP009273/3049071‑3049369
                                                                                                                                                     |                                                                                                                                                     
TGAACCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTA                                                                                                                                                       >  SRR19973066.2984023/1‑150 (MQ=60)
 GAACCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAG                                                                                                                                                      <  SRR19973066.2273666/150‑1 (MQ=60)
      AACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGC                                                                                                                                                 >  SRR19973066.208891/1‑150 (MQ=60)
      AACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGA                                                                                                                                                 >  SRR19973066.2191757/1‑150 (MQ=60)
              CAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAG                                                                                                                                         >  SRR19973066.1048429/1‑150 (MQ=60)
                 GATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACC                                                                                                                                      <  SRR19973066.220041/150‑1 (MQ=60)
                  ATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCA                                                                                                                                     <  SRR19973066.2476815/150‑1 (MQ=60)
                  ATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCA                                                                                                                                     <  SRR19973066.679282/150‑1 (MQ=60)
                  ATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCA                                                                                                                                     <  SRR19973066.676745/150‑1 (MQ=60)
                     TGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAG                                                                                                                                  <  SRR19973066.881612/150‑1 (MQ=60)
                      GAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGA                                                                                                                                 >  SRR19973066.2795492/1‑150 (MQ=60)
                        AAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAAC                                                                                                                               >  SRR19973066.1332384/1‑150 (MQ=60)
                           AACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAG                                                                                                                            >  SRR19973066.2566336/1‑150 (MQ=60)
                           AACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAG                                                                                                                            >  SRR19973066.713821/1‑150 (MQ=60)
                            ACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGC                                                                                                                           <  SRR19973066.423251/150‑1 (MQ=60)
                             CGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCG                                                                                                                          <  SRR19973066.2317033/150‑1 (MQ=60)
                              tctcttactatctAAATACTGAACAGTTGGTATTCATTGCCGCATTGAATATCAGATATGATTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGT                                                                                                                         <  SRR19973066.1932123/137‑1 (MQ=60)
                              gctcttccgatctAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGT                                                                                                                         <  SRR19973066.1082573/137‑1 (MQ=60)
                                ACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTA                                                                                                                       >  SRR19973066.3009617/1‑150 (MQ=60)
                                 CTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTAC                                                                                                                      >  SRR19973066.3471076/1‑150 (MQ=60)
                                 CTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTAC                                                                                                                      >  SRR19973066.3471305/1‑150 (MQ=60)
                                  TAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACT                                                                                                                     <  SRR19973066.286765/150‑1 (MQ=60)
                                   AGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTT                                                                                                                    >  SRR19973066.2793930/1‑150 (MQ=60)
                                   AGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATNGAATATCAGCTATGAGTTAGCGCANGANAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTANGATGCTGCAGCAGACCATAGAACAAGCGTTACTT                                                                                                                    >  SRR19973066.2405876/1‑150 (MQ=60)
                                    GAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTG                                                                                                                   <  SRR19973066.166133/150‑1 (MQ=60)
                                    GAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTG                                                                                                                   <  SRR19973066.2397208/150‑1 (MQ=60)
                                       TCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAAC                                                                                                                <  SRR19973066.733452/150‑1 (MQ=60)
                                             ATACTTAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTC                                                                                                          <  SRR19973066.908966/150‑1 (MQ=60)
                                             ATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAATCAAATACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTC                                                                                                          >  SRR19973066.2335044/1‑150 (MQ=60)
                                             ATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTC                                                                                                          <  SRR19973066.2376495/150‑1 (MQ=60)
                                             ATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTC                                                                                                          <  SRR19973066.2394271/150‑1 (MQ=60)
                                             ATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTC                                                                                                          <  SRR19973066.3572197/150‑1 (MQ=60)
                                             ATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTC                                                                                                          <  SRR19973066.2376327/150‑1 (MQ=60)
                                             ATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTC                                                                                                          <  SRR19973066.3572228/150‑1 (MQ=60)
                                              TACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCG                                                                                                         <  SRR19973066.2901119/150‑1 (MQ=60)
                                              TACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCG                                                                                                         <  SRR19973066.2789666/150‑1 (MQ=60)
                                               ACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGC                                                                                                        >  SRR19973066.3134370/1‑150 (MQ=60)
                                               ACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGC                                                                                                        <  SRR19973066.1225190/150‑1 (MQ=60)
                                               ACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGC                                                                                                        <  SRR19973066.1225289/150‑1 (MQ=60)
                                               ACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGC                                                                                                        <  SRR19973066.1225297/150‑1 (MQ=60)
                                                 TGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCAT                                                                                                      <  SRR19973066.1272002/150‑1 (MQ=60)
                                                 TGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCAT                                                                                                      >  SRR19973066.30638/1‑150 (MQ=60)
                                                   AACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCA                                                                                                    >  SRR19973066.3085502/1‑150 (MQ=60)
                                                      AGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCG                                                                                                 <  SRR19973066.1677404/150‑1 (MQ=60)
                                                         TGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAA                                                                                              >  SRR19973066.381047/1‑150 (MQ=60)
                                                             CTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAAC                                                                                          <  SRR19973066.292453/150‑1 (MQ=60)
                                                              TTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACT                                                                                         <  SRR19973066.1965015/150‑1 (MQ=60)
                                                              TTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACT                                                                                         <  SRR19973066.1792489/150‑1 (MQ=60)
                                                                 ATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAAC                                                                                      <  SRR19973066.2677035/150‑1 (MQ=60)
                                                                  TTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACC                                                                                     <  SRR19973066.3253619/150‑1 (MQ=60)
                                                                   TGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCA                                                                                    >  SRR19973066.2881422/1‑150 (MQ=60)
                                                                   TGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCA                                                                                    >  SRR19973066.1853322/1‑150 (MQ=60)
                                                                   TGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCA                                                                                    >  SRR19973066.2535792/1‑150 (MQ=60)
                                                                       GCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAAC                                                                                <  SRR19973066.830802/150‑1 (MQ=60)
                                                                       GCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAAC                                                                                <  SRR19973066.830715/150‑1 (MQ=60)
                                                                       GCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAAC                                                                                <  SRR19973066.122909/150‑1 (MQ=60)
                                                                        CATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACT                                                                               >  SRR19973066.1464607/1‑150 (MQ=60)
                                                                          TTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACTCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTT                                                                             <  SRR19973066.145804/150‑1 (MQ=60)
                                                                          TTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTT                                                                             <  SRR19973066.935391/150‑1 (MQ=60)
                                                                           TGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAAagatc                                                                            >  SRR19973066.1771174/1‑145 (MQ=60)
                                                                              atttcatctattatTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGAAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAAT                                                                         <  SRR19973066.1316497/136‑1 (MQ=60)
                                                                              ATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAAT                                                                         <  SRR19973066.828981/150‑1 (MQ=60)
                                                                              ATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAAT                                                                         <  SRR19973066.2627132/150‑1 (MQ=60)
                                                                              ATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAAT                                                                         >  SRR19973066.325979/1‑150 (MQ=60)
                                                                                ATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTTCGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGA                                                                       <  SRR19973066.3586123/150‑1 (MQ=60)
                                                                                 TCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGAC                                                                      <  SRR19973066.1956239/150‑1 (MQ=60)
                                                                                 TCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGAC                                                                      <  SRR19973066.1956223/150‑1 (MQ=60)
                                                                                 TCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGAC                                                                      <  SRR19973066.1956243/150‑1 (MQ=60)
                                                                                 TCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGAC                                                                      <  SRR19973066.1119683/150‑1 (MQ=60)
                                                                                 TCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGAC                                                                      <  SRR19973066.1119701/150‑1 (MQ=60)
                                                                                     CTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTT                                                                  >  SRR19973066.3241891/1‑150 (MQ=60)
                                                                                       ATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTT                                                                <  SRR19973066.1165497/150‑1 (MQ=60)
                                                                                       ATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACAGAAAAAACTAACCAAAACTTTGAATGACACTTTT                                                                >  SRR19973066.2452980/1‑150 (MQ=60)
                                                                                        TGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTC                                                               <  SRR19973066.3573331/150‑1 (MQ=60)
                                                                                           GTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGT                                                            >  SRR19973066.2267729/1‑150 (MQ=60)
                                                                                            TTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTT                                                           <  SRR19973066.2296258/150‑1 (MQ=60)
                                                                                             TAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTT                                                          >  SRR19973066.640973/1‑150 (MQ=60)
                                                                                             TAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTT                                                          >  SRR19973066.3546923/1‑150 (MQ=60)
                                                                                             TAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTT                                                          >  SRR19973066.3546845/1‑150 (MQ=60)
                                                                                             TAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTT                                                          <  SRR19973066.1527511/150‑1 (MQ=60)
                                                                                             TAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTT                                                          <  SRR19973066.1523251/150‑1 (MQ=60)
                                                                                             TAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTT                                                          >  SRR19973066.193727/1‑150 (MQ=60)
                                                                                               GCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTAC                                                        <  SRR19973066.219814/150‑1 (MQ=60)
                                                                                                    AGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGG                                                   >  SRR19973066.225521/1‑150 (MQ=60)
                                                                                                     GAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGT                                                  >  SRR19973066.972209/1‑150 (MQ=60)
                                                                                                       AAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAG                                                >  SRR19973066.416810/1‑150 (MQ=60)
                                                                                                       AAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAG                                                >  SRR19973066.2995620/1‑150 (MQ=60)
                                                                                                        AAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGA                                               >  SRR19973066.820680/1‑150 (MQ=60)
                                                                                                        AAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGA                                               >  SRR19973066.351687/1‑150 (MQ=60)
                                                                                                         AAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAG                                              <  SRR19973066.1169786/150‑1 (MQ=60)
                                                                                                          AGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGT                                             <  SRR19973066.578912/150‑1 (MQ=60)
                                                                                                          AGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGT                                             <  SRR19973066.35857/150‑1 (MQ=60)
                                                                                                             AAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAAC                                          <  SRR19973066.1610058/150‑1 (MQ=60)
                                                                                                             AAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAAC                                          <  SRR19973066.1610063/150‑1 (MQ=60)
                                                                                                              AAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACC                                         <  SRR19973066.3308219/150‑1 (MQ=60)
                                                                                                              AAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACC                                         <  SRR19973066.3308228/150‑1 (MQ=60)
                                                                                                              AAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACC                                         <  SRR19973066.2406328/150‑1 (MQ=60)
                                                                                                               AGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCG                                        <  SRR19973066.180134/150‑1 (MQ=60)
                                                                                                               AGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCG                                        <  SRR19973066.2296186/150‑1 (MQ=60)
                                                                                                               AGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCG                                        <  SRR19973066.2295365/150‑1 (MQ=60)
                                                                                                                GACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGT                                       >  SRR19973066.3185114/1‑150 (MQ=60)
                                                                                                                  CTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGA                                     >  SRR19973066.3155704/1‑150 (MQ=60)
                                                                                                                  CTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGA                                     <  SRR19973066.2546593/150‑1 (MQ=60)
                                                                                                                    CGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAG                                   <  SRR19973066.3026921/150‑1 (MQ=60)
                                                                                                                           ACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAAT                            <  SRR19973066.2516853/150‑1 (MQ=60)
                                                                                                                             GCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTT                          <  SRR19973066.2608260/150‑1 (MQ=60)
                                                                                                                               GGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCT                        >  SRR19973066.664606/1‑150 (MQ=60)
                                                                                                                                GCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTC                       <  SRR19973066.2979653/150‑1 (MQ=60)
                                                                                                                                 CAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCT                      >  SRR19973066.348899/1‑150 (MQ=60)
                                                                                                                                   AGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGA                    >  SRR19973066.3138981/1‑150 (MQ=60)
                                                                                                                                   AGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGA                    >  SRR19973066.3139013/1‑150 (MQ=60)
                                                                                                                                   AGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGA                    >  SRR19973066.735774/1‑150 (MQ=60)
                                                                                                                                   AGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGA                    <  SRR19973066.66541/150‑1 (MQ=60)
                                                                                                                                    GTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAG                   >  SRR19973066.758696/1‑150 (MQ=60)
                                                                                                                                    GTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAG                   >  SRR19973066.897522/1‑150 (MQ=60)
                                                                                                                                    GTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAG                   <  SRR19973066.459262/150‑1 (MQ=60)
                                                                                                                                    GTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAG                   <  SRR19973066.459283/150‑1 (MQ=60)
                                                                                                                                     TATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGA                  >  SRR19973066.3186814/1‑150 (MQ=60)
                                                                                                                                      ATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGAT                 <  SRR19973066.1205706/150‑1 (MQ=60)
                                                                                                                                      ATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGAT                 <  SRR19973066.1420179/150‑1 (MQ=60)
                                                                                                                                       TGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATG                >  SRR19973066.3160094/1‑150 (MQ=60)
                                                                                                                                         GAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTT              <  SRR19973066.605794/150‑1 (MQ=60)
                                                                                                                                         GAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTT              <  SRR19973066.3115049/150‑1 (MQ=60)
                                                                                                                                           ACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCG            <  SRR19973066.2458342/150‑1 (MQ=60)
                                                                                                                                             AGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCA          <  SRR19973066.593540/150‑1 (MQ=60)
                                                                                                                                                 TATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCG      >  SRR19973066.2308812/1‑150 (MQ=60)
                                                                                                                                                 TATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCG      >  SRR19973066.2308924/1‑150 (MQ=60)
                                                                                                                                                 TATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCG      >  SRR19973066.2309171/1‑150 (MQ=60)
                                                                                                                                                  ATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGG     >  SRR19973066.109010/1‑150 (MQ=60)
                                                                                                                                                     AGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCC  <  SRR19973066.1208073/150‑1 (MQ=60)
                                                                                                                                                     AGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCC  <  SRR19973066.3346619/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TGAACCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCC  >  CP009273/3049071‑3049369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑