Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,049,220 C→A 100% R84R (CGG→AGG)  zapA → FtsZ stabilizer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,049,2200CA99.5% 672.2 / ‑4.3 203R84R (CGG→AGG) zapAFtsZ stabilizer
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (123/79);  total (124/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 203 total aligned reads displayed.

CCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGC  >  CP009273/3049075‑3049364
                                                                                                                                                 |                                                                                                                                                
  aaCGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGAt                                                                                                                                              >  1:2025461/1‑148 (MQ=255)
   aCGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGAtg                                                                                                                                             >  2:1092010/1‑148 (MQ=255)
   aCGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGAtg                                                                                                                                             >  1:1031040/1‑148 (MQ=255)
      gTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGACCAGTTGGTCTTCAGTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGAtgctg                                                                                                                                          <  2:2790892/148‑1 (MQ=255)
             gATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGa                                                                                                                                   >  1:650599/1‑148 (MQ=255)
             gATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGa                                                                                                                                   <  1:3284781/148‑1 (MQ=255)
                   aaaGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAg                                                                                                                             >  1:3399060/1‑148 (MQ=255)
                    aaGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGa                                                                                                                            >  1:2774125/1‑148 (MQ=255)
                       aaCGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACa                                                                                                                         <  2:3032215/148‑1 (MQ=255)
                                gagTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACt                                                                                                                >  1:1475707/1‑148 (MQ=255)
                                gagTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACt                                                                                                                >  2:1396167/1‑148 (MQ=255)
                                 agTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACtt                                                                                                               <  2:3269618/148‑1 (MQ=255)
                                  gTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTg                                                                                                              >  2:3203398/1‑148 (MQ=255)
                                   tCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGa                                                                                                             <  1:838992/148‑1 (MQ=255)
                                         aTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAgg                                                                                                       >  1:2846248/1‑148 (MQ=255)
                                         aTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAgg                                                                                                       <  2:2748840/148‑1 (MQ=255)
                                         aTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAgg                                                                                                       <  2:2899708/148‑1 (MQ=255)
                                         aTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAgg                                                                                                       >  2:1058594/1‑148 (MQ=255)
                                         aTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAgg                                                                                                       <  2:587457/148‑1 (MQ=255)
                                          tACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGt                                                                                                      >  2:3080705/1‑148 (MQ=255)
                                          tACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGt                                                                                                      <  1:3288520/148‑1 (MQ=255)
                                             tGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGc                                                                                                   >  1:144390/1‑148 (MQ=255)
                                             tGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGc                                                                                                   >  1:1399356/1‑148 (MQ=255)
                                              gAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCa                                                                                                  >  2:916913/1‑148 (MQ=255)
                                              gAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCa                                                                                                  >  1:2354691/1‑148 (MQ=255)
                                                        tCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGaaaa                                                                                        >  1:403586/1‑148 (MQ=255)
                                                        tCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGaaaa                                                                                        >  2:2574677/1‑148 (MQ=255)
                                                         cTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGaaaaa                                                                                       >  1:1136967/1‑148 (MQ=255)
                                                          ttCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGaaaaaa                                                                                      <  1:1039569/148‑1 (MQ=255)
                                                            cATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACt                                                                                    >  2:841717/1‑148 (MQ=255)
                                                              ttGCCGCATAGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTaa                                                                                  >  2:3429393/1‑148 (MQ=255)
                                                               tGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAAc                                                                                 <  2:1768909/148‑1 (MQ=255)
                                                               tGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAAc                                                                                 >  1:1463318/1‑148 (MQ=255)
                                                               tGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAAc                                                                                 >  1:174788/1‑148 (MQ=255)
                                                                 ccGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCa                                                                               >  1:688953/1‑148 (MQ=255)
                                                                   gCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCaaa                                                                             <  2:1494938/148‑1 (MQ=255)
                                                                    cATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCaaaa                                                                            <  1:1699687/148‑1 (MQ=255)
                                                                    cATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCaaaa                                                                            <  2:102934/148‑1 (MQ=255)
                                                                     aTTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAAc                                                                           >  2:1924124/1‑148 (MQ=255)
                                                                     aTTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAAc                                                                           <  1:115083/148‑1 (MQ=255)
                                                                     aTTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAAc                                                                           >  2:2984383/1‑148 (MQ=255)
                                                                     aTTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAAc                                                                           <  1:1889608/148‑1 (MQ=255)
                                                                     aTTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAAc                                                                           >  2:1903619/1‑148 (MQ=255)
                                                                          atatCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGa                                                                      <  1:1498347/148‑1 (MQ=255)
                                                                           tatCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGaa                                                                     >  1:2229361/1‑148 (MQ=255)
                                                                            atCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAAt                                                                    >  2:2651395/1‑148 (MQ=255)
                                                                                gCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGaca                                                                >  2:1956997/1‑148 (MQ=255)
                                                                                gCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGaca                                                                <  1:791515/148‑1 (MQ=255)
                                                                                gCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGaca                                                                >  1:2005308/1‑148 (MQ=255)
                                                                                gCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGaca                                                                <  1:2552863/148‑1 (MQ=255)
                                                                                  tATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACt                                                              >  2:951318/1‑148 (MQ=255)
                                                                                  tATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACt                                                              >  2:1353114/1‑148 (MQ=255)
                                                                                     gAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACtttt                                                           <  1:2289783/148‑1 (MQ=255)
                                                                                       gTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCg                                                         >  2:1426350/1‑148 (MQ=255)
                                                                                        ttAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCgg                                                        >  1:2502005/1‑148 (MQ=255)
                                                                                            cgcAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTa                                                    >  2:2876138/1‑148 (MQ=255)
                                                                                              cAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACt                                                  >  2:2988551/1‑148 (MQ=255)
                                                                                                 gAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACtgtg                                               >  2:2832895/1‑148 (MQ=255)
                                                                                                 gAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACtgtg                                               >  1:157032/1‑148 (MQ=255)
                                                                                                  aaaaaGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTgg                                              >  1:3002764/1‑148 (MQ=255)
                                                                                                  aaaaaGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTgg                                              >  1:3002575/1‑148 (MQ=255)
                                                                                                    aaaGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTa                                            >  1:3075104/1‑148 (MQ=255)
                                                                                                    aaaGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTa                                            >  1:2641374/1‑148 (MQ=255)
                                                                                                    aaaGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTa                                            >  1:1542526/1‑148 (MQ=255)
                                                                                                    aaaGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTa                                            >  1:1422861/1‑148 (MQ=255)
                                                                                                     aaGCAAAGACTCGTGACTACGCGGCAAGTATGGACCAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTag                                           >  2:1236259/1‑148 (MQ=255)
                                                                                                      aGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTaga                                          <  1:1342558/148‑1 (MQ=255)
                                                                                                       gCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTagag                                         <  2:3171431/148‑1 (MQ=255)
                                                                                                         aaaGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTa                                       <  1:1090645/148‑1 (MQ=255)
                                                                                                          aaGACTCGTGACTACGCTGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTaa                                      >  1:942755/1‑148 (MQ=255)
                                                                                                           aGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAAc                                     >  2:3287715/1‑148 (MQ=255)
                                                                                                           aGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAAc                                     >  2:3287764/1‑148 (MQ=255)
                                                                                                             aCTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCg                                   >  2:484513/1‑148 (MQ=255)
                                                                                                              cTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGt                                  <  1:1707868/148‑1 (MQ=255)
                                                                                                                cGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGa                                <  1:1674227/148‑1 (MQ=255)
                                                                                                                  tGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAg                              <  2:2762146/148‑1 (MQ=255)
                                                                                                                  tGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAg                              >  2:624875/1‑148 (MQ=255)
                                                                                                                  tGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAg                              >  1:3513950/1‑148 (MQ=255)
                                                                                                                  tGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAg                              >  1:1823867/1‑148 (MQ=255)
                                                                                                                   gACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACAAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGa                             >  2:250855/1‑148 (MQ=255)
                                                                                                                    aCTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGAc                            >  1:1181012/1‑148 (MQ=255)
                                                                                                                     cTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACa                           >  1:602160/1‑148 (MQ=255)
                                                                                                                     cTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACa                           >  1:2369528/1‑148 (MQ=255)
                                                                                                                        cgcgGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACaaaa                        >  2:11381/1‑148 (MQ=255)
                                                                                                                         gcgGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAAt                       <  2:2221531/148‑1 (MQ=255)
                                                                                                                           ggCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAAttt                     <  2:3123310/148‑1 (MQ=255)
                                                                                                                           ggCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAAttt                     <  1:3203398/148‑1 (MQ=255)
                                                                                                                             cAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTtct                   <  1:3233982/148‑1 (MQ=255)
                                                                                                                               aGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTctct                 >  1:2536110/1‑148 (MQ=255)
                                                                                                                               aGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTctct                 >  1:1272615/1‑148 (MQ=255)
                                                                                                                                  aTGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTgag              <  1:1314053/148‑1 (MQ=255)
                                                                                                                                  aTGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTgag              <  1:1396167/148‑1 (MQ=255)
                                                                                                                                    ggAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGAt            >  2:1637559/1‑148 (MQ=255)
                                                                                                                                     gAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATg           <  2:2005308/148‑1 (MQ=255)
                                                                                                                                         aGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCg       >  1:3058764/1‑148 (MQ=255)
                                                                                                                                           cGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCa     >  2:1301292/1‑148 (MQ=255)
                                                                                                                                           cGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCa     >  2:1301278/1‑148 (MQ=255)
                                                                                                                                              aTTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGc  >  2:683024/1‑148 (MQ=255)
                                                                                                                                              aTTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGc  >  2:683927/1‑148 (MQ=255)
                                                                                                                                              aTTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGc  >  2:1666694/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGC  >  CP009273/3049075‑3049364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGAACCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGC  >  CP009273/3049071‑3049368
                                                                                                                                                     |                                                                                                                                                    
TGAACCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTA                                                                                                                                                      <  SRR19973065.585256/150‑1 (MQ=60)
TGAACCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTA                                                                                                                                                      <  SRR19973065.585373/150‑1 (MQ=60)
  AACCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGG                                                                                                                                                    <  SRR19973065.1638739/150‑1 (MQ=60)
    CCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGAT                                                                                                                                                  >  SRR19973065.881128/1‑150 (MQ=60)
    CCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGAT                                                                                                                                                  >  SRR19973065.2038481/1‑150 (MQ=60)
     CAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATG                                                                                                                                                 >  SRR19973065.1037870/1‑150 (MQ=60)
     CAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATG                                                                                                                                                 >  SRR19973065.2801717/1‑150 (MQ=60)
       ACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCT                                                                                                                                               <  SRR19973065.2618805/150‑1 (MQ=60)
        CGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTG                                                                                                                                              <  SRR19973065.1483007/150‑1 (MQ=60)
             GCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCA                                                                                                                                         <  SRR19973065.3238630/150‑1 (MQ=60)
               AAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGA                                                                                                                                       >  SRR19973065.1703528/1‑150 (MQ=60)
               AAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGA                                                                                                                                       >  SRR19973065.654881/1‑150 (MQ=60)
               AAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGA                                                                                                                                       >  SRR19973065.2235706/1‑150 (MQ=60)
                 GATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACC                                                                                                                                     <  SRR19973065.3304307/150‑1 (MQ=60)
                 GATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACC                                                                                                                                     <  SRR19973065.3304751/150‑1 (MQ=60)
                     TGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAG                                                                                                                                 >  SRR19973065.3419838/1‑150 (MQ=60)
                      GAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGA                                                                                                                                >  SRR19973065.2791352/1‑150 (MQ=60)
                      GAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGA                                                                                                                                >  SRR19973065.3450975/1‑150 (MQ=60)
                             CGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCG                                                                                                                         >  SRR19973065.3326129/1‑150 (MQ=60)
                                  TAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACT                                                                                                                    >  SRR19973065.1485146/1‑150 (MQ=60)
                                     AGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGA                                                                                                                 >  SRR19973065.3142330/1‑150 (MQ=60)
                                     AGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGA                                                                                                                 >  SRR19973065.273533/1‑150 (MQ=60)
                                      GTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAA                                                                                                                >  SRR19973065.127611/1‑150 (MQ=60)
                                       TCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAAC                                                                                                               <  SRR19973065.1537607/150‑1 (MQ=60)
                                       TCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAAC                                                                                                               <  SRR19973065.844455/150‑1 (MQ=60)
                                           AAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGG                                                                                                           >  SRR19973065.2863760/1‑150 (MQ=60)
                                             ATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTC                                                                                                         <  SRR19973065.2553960/150‑1 (MQ=60)
                                              TACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCG                                                                                                        <  SRR19973065.3308496/150‑1 (MQ=60)
                                              TACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCG                                                                                                        <  SRR19973065.3308528/150‑1 (MQ=60)
                                               ACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGC                                                                                                       >  SRR19973065.1456041/1‑150 (MQ=60)
                                               ACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGC                                                                                                       >  SRR19973065.145610/1‑150 (MQ=60)
                                               ACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGC                                                                                                       >  SRR19973065.1408535/1‑150 (MQ=60)
                                                CTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCA                                                                                                      >  SRR19973065.2369335/1‑150 (MQ=60)
                                                CTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCA                                                                                                      >  SRR19973065.2369461/1‑150 (MQ=60)
                                                     CAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACC                                                                                                 <  SRR19973065.1290191/150‑1 (MQ=60)
                                                      AGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCG                                                                                                >  SRR19973065.1361190/1‑150 (MQ=60)
                                                      AGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCG                                                                                                >  SRR19973065.1725468/1‑150 (MQ=60)
                                                      AGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCG                                                                                                >  SRR19973065.302225/1‑150 (MQ=60)
                                                          GGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAA                                                                                            >  SRR19973065.406412/1‑150 (MQ=60)
                                                           GTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAA                                                                                           >  SRR19973065.1144334/1‑150 (MQ=60)
                                                              TTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACT                                                                                        <  SRR19973065.1046449/150‑1 (MQ=60)
                                                                 ATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAAC                                                                                     >  SRR19973065.1472719/1‑150 (MQ=60)
                                                                 ATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAAC                                                                                     >  SRR19973065.176180/1‑150 (MQ=60)
                                                                 ATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAAC                                                                                     <  SRR19973065.1433126/150‑1 (MQ=60)
                                                                   TGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCA                                                                                   >  SRR19973065.693500/1‑150 (MQ=60)
                                                                   TGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCA                                                                                   >  SRR19973065.1759932/1‑150 (MQ=60)
                                                                   TGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAACCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCA                                                                                   >  SRR19973065.693533/1‑150 (MQ=60)
                                                                      CGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAA                                                                                >  SRR19973065.431827/1‑150 (MQ=60)
                                                                        CATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACT                                                                              <  SRR19973065.1711019/150‑1 (MQ=60)
                                                                         ATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTT                                                                             <  SRR19973065.116101/150‑1 (MQ=60)
                                                                         ATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTT                                                                             <  SRR19973065.1902033/150‑1 (MQ=60)
                                                                         ATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTT                                                                             <  SRR19973065.116074/150‑1 (MQ=60)
                                                                             AATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAA                                                                         >  SRR19973065.2243555/1‑150 (MQ=60)
                                                                              ATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAAT                                                                        <  SRR19973065.1508000/150‑1 (MQ=60)
                                                                               TATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATG                                                                       <  SRR19973065.1887965/150‑1 (MQ=60)
                                                                                  CAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACA                                                                    >  SRR19973065.2018265/1‑150 (MQ=60)
                                                                                   AGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACAC                                                                   <  SRR19973065.1121285/150‑1 (MQ=60)
                                                                                    GCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACT                                                                  <  SRR19973065.796758/150‑1 (MQ=60)
                                                                                    GCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACT                                                                  <  SRR19973065.2568839/150‑1 (MQ=60)
                                                                                      TATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTT                                                                >  SRR19973065.269899/1‑150 (MQ=60)
                                                                                         GAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCG                                                             <  SRR19973065.2304204/150‑1 (MQ=60)
                                                                                         GAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCG                                                             <  SRR19973065.2304448/150‑1 (MQ=60)
                                                                                          AGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGG                                                            >  SRR19973065.2294588/1‑150 (MQ=60)
                                                                                          AGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGG                                                            >  SRR19973065.2517731/1‑150 (MQ=60)
                                                                                            TTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTT                                                          <  SRR19973065.1041679/150‑1 (MQ=60)
                                                                                            TTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTT                                                          <  SRR19973065.2534329/150‑1 (MQ=60)
                                                                                             TAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTT                                                         <  SRR19973065.712623/150‑1 (MQ=60)
                                                                                             TAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTT                                                         <  SRR19973065.1923715/150‑1 (MQ=60)
                                                                                                 GCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTG                                                     >  SRR19973065.3067574/1‑150 (MQ=60)
                                                                                                   AAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTG                                                   >  SRR19973065.158333/1‑150 (MQ=60)
                                                                                                    AGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGG                                                  >  SRR19973065.3016321/1‑150 (MQ=60)
                                                                                                    AGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGG                                                  >  SRR19973065.3020968/1‑150 (MQ=60)
                                                                                                    AGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGG                                                  >  SRR19973065.3021160/1‑150 (MQ=60)
                                                                                                      AAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTA                                                >  SRR19973065.1552726/1‑150 (MQ=60)
                                                                                                      AAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTA                                                >  SRR19973065.803019/1‑150 (MQ=60)
                                                                                                      AAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTA                                                >  SRR19973065.1432135/1‑150 (MQ=60)
                                                                                                      AAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTA                                                >  SRR19973065.1432138/1‑150 (MQ=60)
                                                                                                      AAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTA                                                >  SRR19973065.3093892/1‑150 (MQ=60)
                                                                                                      AAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTA                                                >  SRR19973065.2657680/1‑150 (MQ=60)
                                                                                                       AAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAG                                               <  SRR19973065.2473356/150‑1 (MQ=60)
                                                                                                          AGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGT                                            <  SRR19973065.1351438/150‑1 (MQ=60)
                                                                                                            CAAAGACTCGTGACTACGCTGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAA                                          >  SRR19973065.948982/1‑150 (MQ=60)
                                                                                                             AAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAAC                                         <  SRR19973065.1097832/150‑1 (MQ=60)
                                                                                                             AAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTAGGTTTACTGTGGTAGAGTAAC                                         <  SRR19973065.1046492/150‑1 (MQ=60)
                                                                                                                 ACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAATTCTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTG                                     >  SRR19973065.1663962/1‑150 (MQ=60)
                                                                                                                  CTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGA                                    <  SRR19973065.1719230/150‑1 (MQ=60)
                                                                                                                    CGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAG                                  >  SRR19973065.1835878/1‑150 (MQ=60)
                                                                                                                    CGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAG                                  >  SRR19973065.3535254/1‑150 (MQ=60)
                                                                                                                    CGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAG                                  <  SRR19973065.1685426/150‑1 (MQ=60)
                                                                                                                    CGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGATACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGAGGTAGAGTCACCGTGAAG                                  >  SRR19973065.1491976/1‑150 (MQ=60)
                                                                                                                      TGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGAC                                >  SRR19973065.1188496/1‑150 (MQ=60)
                                                                                                                      TGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGAC                                >  SRR19973065.370774/1‑150 (MQ=60)
                                                                                                                       GACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACA                               >  SRR19973065.2384303/1‑150 (MQ=60)
                                                                                                                       GACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACA                               >  SRR19973065.605936/1‑150 (MQ=60)
                                                                                                                               GGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCT                       >  SRR19973065.1164725/1‑150 (MQ=60)
                                                                                                                               GGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCT                       <  SRR19973065.3222683/150‑1 (MQ=60)
                                                                                                                                 CAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCT                     >  SRR19973065.2552018/1‑150 (MQ=60)
                                                                                                                                 CAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCT                     >  SRR19973065.1281009/1‑150 (MQ=60)
                                                                                                                                 CAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCT                     <  SRR19973065.3253429/150‑1 (MQ=60)
                                                                                                                                      ATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGAT                <  SRR19973065.3078218/150‑1 (MQ=60)
                                                                                                                                      ATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGAT                <  SRR19973065.1322743/150‑1 (MQ=60)
                                                                                                                                      ATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGAT                <  SRR19973065.1405332/150‑1 (MQ=60)
                                                                                                                                      ATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGAT                <  SRR19973065.1405345/150‑1 (MQ=60)
                                                                                                                                        GGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGT              <  SRR19973065.1349995/150‑1 (MQ=60)
                                                                                                                                        GGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGT              >  SRR19973065.626361/1‑150 (MQ=60)
                                                                                                                                          AACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTC            <  SRR19973065.1553565/150‑1 (MQ=60)
                                                                                                                                           ACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCG           >  SRR19973065.3077460/1‑150 (MQ=60)
                                                                                                                                             AGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCA         >  SRR19973065.2023160/1‑150 (MQ=60)
                                                                                                                                             AGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCA         >  SRR19973065.1714551/1‑150 (MQ=60)
                                                                                                                                                 TATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCG     >  SRR19973065.466403/1‑150 (MQ=60)
                                                                                                                                                 TATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCG     >  SRR19973065.1377625/1‑150 (MQ=60)
                                                                                                                                                 TATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCG     >  SRR19973065.3492698/1‑150 (MQ=60)
                                                                                                                                                    TAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGC  <  SRR19973065.2013107/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TGAACCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGC  >  CP009273/3049071‑3049368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑