Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,049,220 C→A 100% R84R (CGG→AGG)  zapA → FtsZ stabilizer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,049,2200CA98.9% 862.3 / 0.3 263R84R (CGG→AGG) zapAFtsZ stabilizer
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base A (122/138);  total (122/141)
Fisher's exact test for biased strand distribution p-value = 2.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 263 total aligned reads displayed.

CCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGC  >  CP009273/3049075‑3049364
                                                                                                                                                 |                                                                                                                                                
ccAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAgg                                                                                                                                                <  2:53958/148‑1 (MQ=255)
ccAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAgg                                                                                                                                                <  2:1757170/148‑1 (MQ=255)
  aaCGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGAt                                                                                                                                              >  2:3307026/1‑148 (MQ=255)
   aCGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGAtg                                                                                                                                             >  2:1728839/1‑148 (MQ=255)
    ctgTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGAtgc                                                                                                                                            <  1:2096362/146‑1 (MQ=255)
    cGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGAtgc                                                                                                                                            <  1:2096830/148‑1 (MQ=255)
      gTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGAtgctg                                                                                                                                          >  1:337964/1‑148 (MQ=255)
         gCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTgcag                                                                                                                                       <  1:1253196/148‑1 (MQ=255)
             gATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGa                                                                                                                                   >  2:1239004/1‑148 (MQ=255)
             gATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGa                                                                                                                                   >  1:889754/1‑148 (MQ=255)
              aTCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGAc                                                                                                                                  >  2:943539/1‑148 (MQ=255)
                  gAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATa                                                                                                                              >  1:1461659/1‑148 (MQ=255)
                  gAAAGAACGCACTAGAGTCACAAATAATGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATa                                                                                                                              >  2:2276078/1‑148 (MQ=255)
                    aaGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGa                                                                                                                            >  1:2457635/1‑148 (MQ=255)
                      gAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAAc                                                                                                                          >  1:1796364/1‑148 (MQ=255)
                       aaCGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACa                                                                                                                         <  1:1776717/148‑1 (MQ=255)
                       aaCGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACa                                                                                                                         <  2:574097/148‑1 (MQ=255)
                       aaCGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACa                                                                                                                         <  1:2631715/148‑1 (MQ=255)
                        aCGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACaa                                                                                                                        <  1:3001132/148‑1 (MQ=255)
                             cTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGtt                                                                                                                   >  1:2052468/1‑148 (MQ=255)
                             cTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGtt                                                                                                                   >  2:636301/1‑148 (MQ=255)
                              tAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTa                                                                                                                  <  2:2750903/148‑1 (MQ=255)
                              tAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTa                                                                                                                  >  2:366221/1‑148 (MQ=255)
                              tAGAGTCAAAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTa                                                                                                                  <  1:1304553/148‑1 (MQ=255)
                                   tCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGa                                                                                                             <  2:3375715/148‑1 (MQ=255)
                                    cacaAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGaa                                                                                                            <  2:1184594/148‑1 (MQ=255)
                                     acaAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAAc                                                                                                           <  1:3794406/148‑1 (MQ=255)
                                        aaTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAg                                                                                                        >  1:3573895/1‑148 (MQ=255)
                                         aTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAgg                                                                                                       <  2:1152179/148‑1 (MQ=255)
                                         aTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAgg                                                                                                       <  2:3074495/148‑1 (MQ=255)
                                         aTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAgg                                                                                                       >  2:592335/1‑148 (MQ=255)
                                         aTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAgg                                                                                                       >  2:2575355/1‑148 (MQ=255)
                                         aTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAgg                                                                                                       <  1:2476285/148‑1 (MQ=255)
                                         aTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAgg                                                                                                       <  2:532600/148‑1 (MQ=255)
                                           aCTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTc                                                                                                     >  2:2793040/1‑148 (MQ=255)
                                               aaCAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCAt                                                                                                 <  1:2081389/148‑1 (MQ=255)
                                               aaCAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCAt                                                                                                 <  1:2945686/148‑1 (MQ=255)
                                                 cAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCa                                                                                               >  2:1558034/1‑148 (MQ=255)
                                                  aGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCAc                                                                                              <  2:3233034/148‑1 (MQ=255)
                                                     tGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCACGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGa                                                                                           >  1:1962656/1‑148 (MQ=255)
                                                      ggTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGaa                                                                                          <  1:579781/148‑1 (MQ=255)
                                                         cTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGaaaaa                                                                                       >  1:764621/1‑148 (MQ=255)
                                                          ttCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGaaaaaa                                                                                      <  2:2689737/148‑1 (MQ=255)
                                                             aTTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTa                                                                                   <  2:144536/148‑1 (MQ=255)
                                                             aTTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTa                                                                                   <  2:3716148/148‑1 (MQ=255)
                                                              ttGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTaa                                                                                  >  2:3217533/1‑148 (MQ=255)
                                                               tGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCATCGAAAAAACTAAc                                                                                 <  2:1662524/148‑1 (MQ=255)
                                                                   gCATTTAATTTCATGTATTAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCaaa                                                                             <  2:80044/148‑1 (MQ=255)
                                                                   gCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCaaa                                                                             <  2:1367586/148‑1 (MQ=255)
                                                                    cATTTAATATCATCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACATCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCaaaa                                                                            <  2:3592509/148‑1 (MQ=255)
                                                                    cATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCaaaa                                                                            <  1:636301/148‑1 (MQ=255)
                                                                     aTTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAAc                                                                           <  1:2024435/148‑1 (MQ=255)
                                                                      ttGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACt                                                                          >  2:1141525/1‑148 (MQ=255)
                                                                         aaTATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTg                                                                       <  2:3807489/148‑1 (MQ=255)
                                                                          atatCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGa                                                                      >  1:833170/1‑148 (MQ=255)
                                                                          atatCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGa                                                                      >  2:2937252/1‑148 (MQ=255)
                                                                          atatCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGa                                                                      >  2:599822/1‑148 (MQ=255)
                                                                            atCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAAt                                                                    <  2:2014112/148‑1 (MQ=255)
                                                                            atCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAAt                                                                    >  2:133804/1‑148 (MQ=255)
                                                                             tCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATg                                                                   <  2:1576393/148‑1 (MQ=255)
                                                                             tCAGCTATGAGTTAGCGAAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATg                                                                   <  2:303707/148‑1 (MQ=255)
                                                                                gCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGaca                                                                <  1:1208601/148‑1 (MQ=255)
                                                                                gCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGaca                                                                <  1:3399172/148‑1 (MQ=255)
                                                                                 cTATGAGCTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGacac                                                               >  1:1031658/1‑148 (MQ=255)
                                                                                   aTGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACtt                                                             >  1:3508919/1‑148 (MQ=255)
                                                                                     gAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACtttt                                                           <  2:3573835/148‑1 (MQ=255)
                                                                                     gAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACtttt                                                           <  1:266808/148‑1 (MQ=255)
                                                                                         tAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGt                                                       <  2:2275583/148‑1 (MQ=255)
                                                                                         tAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGt                                                       <  1:2793040/148‑1 (MQ=255)
                                                                                          aGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGTCAAGTATGTAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGtt                                                      <  2:2148748/148‑1 (MQ=255)
                                                                                          aGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGtt                                                      <  2:405183/148‑1 (MQ=255)
                                                                                                 gAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACtgtg                                               >  1:1340703/1‑148 (MQ=255)
                                                                                                 gAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACtgtg                                               >  2:3135515/1‑148 (MQ=255)
                                                                                                  aaaaaGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTgg                                              <  1:1620521/148‑1 (MQ=255)
                                                                                                     aaGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTag                                           >  1:99222/1‑148 (MQ=255)
                                                                                                      aGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTaga                                          <  1:736837/148‑1 (MQ=255)
                                                                                                       gCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTagag                                         >  2:1900576/1‑148 (MQ=255)
                                                                                                       gCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTagag                                         <  2:2457581/148‑1 (MQ=255)
                                                                                                       gCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACAATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTagag                                         <  2:2621537/148‑1 (MQ=255)
                                                                                                        cAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGTTTTACTGTGGTAGAGt                                        >  1:314343/1‑148 (MQ=255)
                                                                                                        cAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGt                                        >  1:314846/1‑148 (MQ=255)
                                                                                                         aaaGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTa                                       <  1:1184760/148‑1 (MQ=255)
                                                                                                              cTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGt                                  <  1:3613519/148‑1 (MQ=255)
                                                                                                                cGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGa                                >  2:2207461/1‑148 (MQ=255)
                                                                                                                  tGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAg                              <  1:1661165/148‑1 (MQ=255)
                                                                                                                  tGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAg                              <  1:1512753/148‑1 (MQ=255)
                                                                                                                     cTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACa                           >  1:2185076/1‑148 (MQ=255)
                                                                                                                        cgcgGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACaaaa                        <  2:3539369/148‑1 (MQ=255)
                                                                                                                             cAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTtct                   <  2:3642327/148‑1 (MQ=255)
                                                                                                                              aaGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTtctc                  <  1:1079194/148‑1 (MQ=255)
                                                                                                                              aaGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTtctc                  <  1:3733991/148‑1 (MQ=255)
                                                                                                                                  aTGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTCGAGTAACCGTGAAGACAAAATTTCTCTgag              >  1:413240/1‑148 (MQ=255)
                                                                                                                                  aTGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTgag              <  2:3481904/148‑1 (MQ=255)
                                                                                                                                  aTGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTgag              <  1:994236/148‑1 (MQ=255)
                                                                                                                                  aTGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTgag              >  2:2464500/1‑148 (MQ=255)
                                                                                                                                  aTGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTgag              <  2:1584057/148‑1 (MQ=255)
                                                                                                                                    ggAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGAt            >  1:2542697/1‑148 (MQ=255)
                                                                                                                                    ggAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGAt            >  1:1308951/1‑148 (MQ=255)
                                                                                                                                         aGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCg       <  2:586256/148‑1 (MQ=255)
                                                                                                                                           cGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACACAATTTCTCTGAGATGTTCGCa     >  2:2341030/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGC  >  CP009273/3049075‑3049364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAACCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCG  >  CP009273/3049072‑3049365
                                                                                                                                                    |                                                                                                                                                 
GAACCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAG                                                                                                                                                  <  SRR19973069.3180813/150‑1 (MQ=60)
 AACCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGG                                                                                                                                                 <  SRR19973069.1682487/150‑1 (MQ=60)
   CCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGAT                                                                                                                                               >  SRR19973069.3521436/1‑150 (MQ=60)
      ACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCT                                                                                                                                            <  SRR19973069.3613930/150‑1 (MQ=60)
      ACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCT                                                                                                                                            <  SRR19973069.1321618/150‑1 (MQ=60)
       CTGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTG                                                                                                                                           <  SRR19973069.2110848/150‑1 (MQ=60)
       CGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTG                                                                                                                                           <  SRR19973069.2110857/150‑1 (MQ=60)
       CGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTG                                                                                                                                           >  SRR19973069.340326/1‑150 (MQ=60)
       CGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTG                                                                                                                                           <  SRR19973069.2111316/150‑1 (MQ=60)
        GGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGC                                                                                                                                          >  SRR19973069.2047539/1‑150 (MQ=60)
        GGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGC                                                                                                                                          >  SRR19973069.1426965/1‑150 (MQ=60)
            GCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCA                                                                                                                                      <  SRR19973069.1261672/150‑1 (MQ=60)
              AAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGA                                                                                                                                    >  SRR19973069.895797/1‑150 (MQ=60)
              AAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGA                                                                                                                                    >  SRR19973069.895899/1‑150 (MQ=60)
              AAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGA                                                                                                                                    >  SRR19973069.901655/1‑150 (MQ=60)
                   CTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATA                                                                                                                               >  SRR19973069.1471861/1‑150 (MQ=60)
                    TGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAG                                                                                                                              >  SRR19973069.3312321/1‑150 (MQ=60)
                    TGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAG                                                                                                                              <  SRR19973069.2052593/150‑1 (MQ=60)
                     GAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGA                                                                                                                             >  SRR19973069.540984/1‑150 (MQ=60)
                     GAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGA                                                                                                                             >  SRR19973069.2474095/1‑150 (MQ=60)
                     GAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGA                                                                                                                             >  SRR19973069.2474149/1‑150 (MQ=60)
                       AAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAAC                                                                                                                           >  SRR19973069.1809016/1‑150 (MQ=60)
                        AGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACA                                                                                                                          >  SRR19973069.2187642/1‑150 (MQ=60)
                        AGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACA                                                                                                                          >  SRR19973069.2186537/1‑150 (MQ=60)
                          AACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAG                                                                                                                        <  SRR19973069.2649348/150‑1 (MQ=60)
                          AACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAG                                                                                                                        <  SRR19973069.2649339/150‑1 (MQ=60)
                          AACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAG                                                                                                                        >  SRR19973069.1301438/1‑150 (MQ=60)
                          AACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAG                                                                                                                        <  SRR19973069.1789231/150‑1 (MQ=60)
                          AACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAG                                                                                                                        >  SRR19973069.2094848/1‑150 (MQ=60)
                           ACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGC                                                                                                                       <  SRR19973069.3021049/150‑1 (MQ=60)
                              CACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTT                                                                                                                    >  SRR19973069.2066712/1‑150 (MQ=60)
                                 TAGAGTCAAAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACT                                                                                                                 <  SRR19973069.1313412/150‑1 (MQ=60)
                                       CACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACA                                                                                                           <  SRR19973069.3747747/150‑1 (MQ=60)
                                        ACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAA                                                                                                          <  SRR19973069.3819000/150‑1 (MQ=60)
                                         CAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAG                                                                                                         >  SRR19973069.2736425/1‑150 (MQ=60)
                                         CAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAG                                                                                                         >  SRR19973069.3597189/1‑150 (MQ=60)
                                         CAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAG                                                                                                         <  SRR19973069.1253097/150‑1 (MQ=60)
                                          AAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGG                                                                                                        >  SRR19973069.1481916/1‑150 (MQ=60)
                                          AAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGG                                                                                                        >  SRR19973069.340933/1‑150 (MQ=60)
                                            ATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTC                                                                                                      >  SRR19973069.1768256/1‑150 (MQ=60)
                                            ATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTC                                                                                                      <  SRR19973069.2492880/150‑1 (MQ=60)
                                            ATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTC                                                                                                      >  SRR19973069.1332354/1‑150 (MQ=60)
                                            ATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTC                                                                                                      <  SRR19973069.2490376/150‑1 (MQ=60)
                                                 GAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATC                                                                                                 <  SRR19973069.75973/150‑1 (MQ=60)
                                                 GAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATC                                                                                                 <  SRR19973069.3774783/150‑1 (MQ=60)
                                                 GAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATC                                                                                                 <  SRR19973069.3775276/150‑1 (MQ=60)
                                                 GAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATC                                                                                                 <  SRR19973069.75960/150‑1 (MQ=60)
                                                  AACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCA                                                                                                <  SRR19973069.2965231/150‑1 (MQ=60)
                                                  AACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCA                                                                                                <  SRR19973069.2095793/150‑1 (MQ=60)
                                                  AACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCA                                                                                                <  SRR19973069.2095798/150‑1 (MQ=60)
                                                  AACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCA                                                                                                <  SRR19973069.2095823/150‑1 (MQ=60)
                                                      GTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCACGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGA                                                                                            >  SRR19973069.1976262/1‑150 (MQ=60)
                                                      GTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGA                                                                                            >  SRR19973069.1976261/1‑150 (MQ=60)
                                                         GGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAA                                                                                         <  SRR19973069.583762/150‑1 (MQ=60)
                                                          GTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAA                                                                                        >  SRR19973069.3562839/1‑150 (MQ=60)
                                                          GTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAA                                                                                        >  SRR19973069.1570438/1‑150 (MQ=60)
                                                          GTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAA                                                                                        >  SRR19973069.770016/1‑150 (MQ=60)
                                                          GTCTTCATTGCCGCAGTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAA                                                                                        >  SRR19973069.3562330/1‑150 (MQ=60)
                                                            CTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAAC                                                                                      <  SRR19973069.1463378/150‑1 (MQ=60)
                                                               CATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAA                                                                                   >  SRR19973069.2920464/1‑150 (MQ=60)
                                                               CATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAA                                                                                   >  SRR19973069.2010676/1‑150 (MQ=60)
                                                                ATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAAC                                                                                  >  SRR19973069.3832138/1‑150 (MQ=60)
                                                                  TGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCA                                                                                <  SRR19973069.286126/150‑1 (MQ=60)
                                                                       CATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACT                                                                           <  SRR19973069.640491/150‑1 (MQ=60)
                                                                       CATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACT                                                                           <  SRR19973069.640400/150‑1 (MQ=60)
                                                                       CATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACT                                                                           <  SRR19973069.3623486/150‑1 (MQ=60)
                                                                        ATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTT                                                                          <  SRR19973069.2038494/150‑1 (MQ=60)
                                                                          TGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTG                                                                        <  SRR19973069.607812/150‑1 (MQ=60)
                                                                          TGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTG                                                                        <  SRR19973069.3408355/150‑1 (MQ=60)
                                                                           GAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGA                                                                       >  SRR19973069.839036/1‑150 (MQ=60)
                                                                           GAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGA                                                                       >  SRR19973069.838842/1‑150 (MQ=60)
                                                                                  AGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACAC                                                                <  SRR19973069.3023633/150‑1 (MQ=60)
                                                                                  AGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACAC                                                                >  SRR19973069.179586/1‑150 (MQ=60)
                                                                                  AGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACAC                                                                >  SRR19973069.179591/1‑150 (MQ=60)
                                                                                  AGCTATGAGCTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACAC                                                                >  SRR19973069.1038801/1‑150 (MQ=60)
                                                                                   GCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACT                                                               <  SRR19973069.3421352/150‑1 (MQ=60)
                                                                                   GCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACT                                                               <  SRR19973069.1216900/150‑1 (MQ=60)
                                                                                    CTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTT                                                              >  SRR19973069.3531506/1‑150 (MQ=60)
                                                                                    CTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTT                                                              >  SRR19973069.262397/1‑150 (MQ=60)
                                                                                    CTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTT                                                              >  SRR19973069.262310/1‑150 (MQ=60)
                                                                                     TATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTT                                                             <  SRR19973069.297860/150‑1 (MQ=60)
                                                                                        GAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCG                                                          <  SRR19973069.269508/150‑1 (MQ=60)
                                                                                        GAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCG                                                          <  SRR19973069.268712/150‑1 (MQ=60)
                                                                                            TAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTT                                                      <  SRR19973069.2811611/150‑1 (MQ=60)
                                                                                            TAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTT                                                      <  SRR19973069.3089251/150‑1 (MQ=60)
                                                                                                  AAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTG                                                >  SRR19973069.1349964/1‑150 (MQ=60)
                                                                                                   AGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGG                                               <  SRR19973069.1692025/150‑1 (MQ=60)
                                                                                                   AGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGG                                               <  SRR19973069.1247434/150‑1 (MQ=60)
                                                                                                     AAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTA                                             <  SRR19973069.1631509/150‑1 (MQ=60)
                                                                                                     AAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTA                                             <  SRR19973069.1632125/150‑1 (MQ=60)
                                                                                                      AAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAG                                            <  SRR19973069.2518085/150‑1 (MQ=60)
                                                                                                      AAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAG                                            >  SRR19973069.100219/1‑150 (MQ=60)
                                                                                                      AAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAG                                            >  SRR19973069.100222/1‑150 (MQ=60)
                                                                                                         AGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGTTTTACTGTGGTAGAGT                                         >  SRR19973069.316429/1‑150 (MQ=60)
                                                                                                         AGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGT                                         <  SRR19973069.742054/150‑1 (MQ=60)
                                                                                                         AGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGT                                         >  SRR19973069.316934/1‑150 (MQ=60)
                                                                                                         AGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGT                                         <  SRR19973069.740274/150‑1 (MQ=60)
                                                                                                            AAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAAC                                      >  SRR19973069.1117551/1‑150 (MQ=60)
                                                                                                            AAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAAC                                      <  SRR19973069.1191708/150‑1 (MQ=60)
                                                                                                            AAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAAC                                      >  SRR19973069.3263241/1‑150 (MQ=60)
                                                                                                            AAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAAC                                      <  SRR19973069.1192942/150‑1 (MQ=60)
                                                                                                                ACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTG                                  >  SRR19973069.2026680/1‑150 (MQ=60)
                                                                                                                 CTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGA                                 <  SRR19973069.3637115/150‑1 (MQ=60)
                                                                                                                     TGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGAC                             <  SRR19973069.1672673/150‑1 (MQ=60)
                                                                                                                     TGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGAC                             <  SRR19973069.1593847/150‑1 (MQ=60)
                                                                                                                     TGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGAC                             <  SRR19973069.1523205/150‑1 (MQ=60)
                                                                                                                      GACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACA                            >  SRR19973069.2199958/1‑150 (MQ=60)
                                                                                                                             CGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTC                     >  SRR19973069.2499571/1‑150 (MQ=60)
                                                                                                                                 AAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTG                 <  SRR19973069.3758333/150‑1 (MQ=60)
                                                                                                                                 AAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTG                 <  SRR19973069.1086354/150‑1 (MQ=60)
                                                                                                                                 AAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTG                 <  SRR19973069.1086725/150‑1 (MQ=60)
                                                                                                                                   GTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTCGAGTAACCGTGAAGACAAAATTTCTCTGAG               >  SRR19973069.416266/1‑150 (MQ=60)
                                                                                                                                   GTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAG               >  SRR19973069.2355234/1‑150 (MQ=60)
                                                                                                                                   GTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAG               >  SRR19973069.1196809/1‑150 (MQ=60)
                                                                                                                                     ATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCTGTTTAATGTGGTAGAGTAACAGTGAAGACAAAATTTCTCTGAGAT             >  SRR19973069.1306003/1‑150 (MQ=60)
                                                                                                                                     ATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGAT             >  SRR19973069.2559520/1‑150 (MQ=60)
                                                                                                                                     ATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGAT             <  SRR19973069.1001006/150‑1 (MQ=60)
                                                                                                                                     ATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGAT             >  SRR19973069.1317893/1‑150 (MQ=60)
                                                                                                                                     ATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGAT             >  SRR19973069.1317803/1‑150 (MQ=60)
                                                                                                                                        GAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTT          <  SRR19973069.490418/150‑1 (MQ=60)
                                                                                                                                        GAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTT          <  SRR19973069.836909/150‑1 (MQ=60)
                                                                                                                                        GAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTT          <  SRR19973069.949901/150‑1 (MQ=60)
                                                                                                                                              CGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAG    <  SRR19973069.3164266/150‑1 (MQ=60)
                                                                                                                                                TATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCG  >  SRR19973069.3597818/1‑150 (MQ=60)
                                                                                                                                                TATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCG  >  SRR19973069.3597843/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                 
GAACCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCG  >  CP009273/3049072‑3049365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑