Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,049,220 C→A 100% R84R (CGG→AGG)  zapA → FtsZ stabilizer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,049,2200CA97.7% 846.4 / 6.2 265R84R (CGG→AGG) zapAFtsZ stabilizer
Reads supporting (aligned to +/- strand):  ref base C (2/4);  new base A (143/114);  total (146/119)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 265 total aligned reads displayed.

AACCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGG  >  CP009273/3049073‑3049367
                                                                                                                                                   |                                                                                                                                                   
 aCCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGa                                                                                                                                                  >  2:3735363/1‑150 (MQ=255)
    aaCGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGAtgc                                                                                                                                               >  2:1521417/1‑150 (MQ=255)
     aCGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGAtgct                                                                                                                                              <  2:1083444/150‑1 (MQ=255)
      cGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGAtgctg                                                                                                                                             <  2:851396/150‑1 (MQ=255)
        gTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTgca                                                                                                                                           >  2:2653767/1‑150 (MQ=255)
         ttGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGTACAGCGTATTAGGATGCTgcag                                                                                                                                          >  1:2963352/1‑150 (MQ=255)
         ttGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTgcag                                                                                                                                          >  2:1353749/1‑150 (MQ=255)
           gCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTgcagca                                                                                                                                        >  1:3229938/1‑150 (MQ=255)
             aaGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGa                                                                                                                                      >  1:286917/1‑150 (MQ=255)
             aaGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGATGCAGCAGa                                                                                                                                      >  1:3134663/1‑150 (MQ=255)
              aGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGAc                                                                                                                                     <  1:4221743/150‑1 (MQ=255)
                 tCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCAt                                                                                                                                  >  1:3618999/1‑150 (MQ=255)
                    gAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGa                                                                                                                               >  2:4940161/1‑150 (MQ=255)
                    gAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGa                                                                                                                               >  1:2084627/1‑150 (MQ=255)
                     aaaGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGaa                                                                                                                              >  2:4813050/1‑150 (MQ=255)
                     aaaGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGaa                                                                                                                              >  1:1062737/1‑150 (MQ=255)
                     aaaGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGaa                                                                                                                              >  1:4661582/1‑150 (MQ=255)
                     aaaGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGaa                                                                                                                              >  1:4495864/1‑150 (MQ=255)
                      aaGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAAc                                                                                                                             >  1:1503078/1‑150 (MQ=255)
                       aGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACa                                                                                                                            >  2:2502212/1‑150 (MQ=255)
                        gAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACaa                                                                                                                           >  1:4866706/1‑150 (MQ=255)
                          aCGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGc                                                                                                                         <  2:3581748/150‑1 (MQ=255)
                          aCGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGc                                                                                                                         <  2:2407793/150‑1 (MQ=255)
                             cACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGtt                                                                                                                      >  1:2108771/1‑150 (MQ=255)
                               cTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTAc                                                                                                                    <  1:1356765/150‑1 (MQ=255)
                                tAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACt                                                                                                                   >  1:673531/1‑150 (MQ=255)
                                     tCACAAATACTGACCAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAAc                                                                                                              <  2:4365836/150‑1 (MQ=255)
                                      cacaAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACa                                                                                                             <  1:3443690/150‑1 (MQ=255)
                                       acaAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACaa                                                                                                            <  2:1931878/150‑1 (MQ=255)
                                          aaTACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGt                                                                                                         >  2:3275254/1‑150 (MQ=255)
                                                 aaCAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCa                                                                                                  >  1:4700455/1‑150 (MQ=255)
                                                   cAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCAcc                                                                                                <  1:2574214/150‑1 (MQ=255)
                                                    aGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCg                                                                                               >  1:1406425/1‑150 (MQ=255)
                                                    aGTTGGTCTTCAGTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGGAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCg                                                                                               <  2:3430838/150‑1 (MQ=255)
                                                     gTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGa                                                                                              <  2:738968/150‑1 (MQ=255)
                                                     gTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGa                                                                                              >  1:1667142/1‑150 (MQ=255)
                                                      ttGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGaa                                                                                             <  1:3820273/150‑1 (MQ=255)
                                                      ttGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGaa                                                                                             <  2:1236435/150‑1 (MQ=255)
                                                          tCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGAATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGaaaaaa                                                                                         >  1:4591577/1‑150 (MQ=255)
                                                               aTTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAAc                                                                                    >  2:3058601/1‑150 (MQ=255)
                                                               aTTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAAc                                                                                    >  1:2189706/1‑150 (MQ=255)
                                                                ttGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAAcc                                                                                   <  2:2760088/150‑1 (MQ=255)
                                                                 tGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCa                                                                                  <  2:4185824/150‑1 (MQ=255)
                                                                  gCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCaa                                                                                 <  2:1937285/150‑1 (MQ=255)
                                                                    cGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCaaaa                                                                               >  1:1173321/1‑150 (MQ=255)
                                                                    cGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCaaaa                                                                               >  2:287380/1‑150 (MQ=255)
                                                                    cGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCaaaa                                                                               >  2:1134825/1‑150 (MQ=255)
                                                                      cATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACt                                                                             <  1:4411025/150‑1 (MQ=255)
                                                                      cATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACt                                                                             >  2:295341/1‑150 (MQ=255)
                                                                       aTTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACtt                                                                            <  2:534141/150‑1 (MQ=255)
                                                                        ttGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACttt                                                                           <  2:3000034/150‑1 (MQ=255)
                                                                           aaTATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGaa                                                                        <  1:769227/150‑1 (MQ=255)
                                                                            atatCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAAt                                                                       >  2:2468001/1‑150 (MQ=255)
                                                                             tatCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATg                                                                      >  1:4724586/1‑150 (MQ=255)
                                                                              atCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGa                                                                     <  2:3817448/150‑1 (MQ=255)
                                                                                 aGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGATCAAGGTCGCATCACCGAAAAAACTAACCAAAACTTGGAATGacac                                                                  >  2:4667116/1‑150 (MQ=255)
                                                                                 aGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGacac                                                                  >  2:4346909/1‑150 (MQ=255)
                                                                                   cTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACtt                                                                <  1:1998685/150‑1 (MQ=255)
                                                                                    tATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACttt                                                               <  1:1720245/150‑1 (MQ=255)
                                                                                     aTGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACtttt                                                              <  1:2341744/150‑1 (MQ=255)
                                                                                     aTGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACtttt                                                              >  1:3898359/1‑150 (MQ=255)
                                                                                        aGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTGGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCgg                                                           >  2:512864/1‑150 (MQ=255)
                                                                                        aGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCgg                                                           >  1:688498/1‑150 (MQ=255)
                                                                                         gTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGt                                                          >  1:561520/1‑150 (MQ=255)
                                                                                          ttAGCGCAAGAAAAGGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGtt                                                         <  2:3144025/150‑1 (MQ=255)
                                                                                          ttAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGtt                                                         >  2:399040/1‑150 (MQ=255)
                                                                                           tAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGttt                                                        <  2:550590/150‑1 (MQ=255)
                                                                                           tAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGttt                                                        >  2:4555846/1‑150 (MQ=255)
                                                                                            aGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTa                                                       <  2:2569031/150‑1 (MQ=255)
                                                                                            aGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTa                                                       >  2:1574946/1‑150 (MQ=255)
                                                                                               gcAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACtg                                                    <  1:2728490/150‑1 (MQ=255)
                                                                                                cAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACtgt                                                   >  2:118540/1‑150 (MQ=255)
                                                                                                cAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACtgt                                                   >  1:1882067/1‑150 (MQ=255)
                                                                                                   gAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGt                                                >  2:2101628/1‑150 (MQ=255)
                                                                                                    aaaaaGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTa                                               >  1:3548797/1‑150 (MQ=255)
                                                                                                     aaaaGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTag                                              >  1:86778/1‑150 (MQ=255)
                                                                                                     aaaaGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTag                                              <  2:3193628/150‑1 (MQ=255)
                                                                                                     aaaaGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTag                                              >  2:3498721/1‑150 (MQ=255)
                                                                                                     aaaaGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTag                                              >  1:517725/1‑150 (MQ=255)
                                                                                                      aaaGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTaga                                             >  2:2160783/1‑150 (MQ=255)
                                                                                                        agctacgACTCGTGACTACGCGGCATGTATGGATCAGCGTATTAGGATGCTGCAGCAGACCATAGATCTTGCGTTACTTGAACAAGGTGGCTTGTCCGTAAAATCTAACCCACCCTTTGAATGACACGTTTCGGTTTACTGTGGTAGAGt                                           <  2:2623592/144‑1 (MQ=255)
                                                                                                           aaaGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAAc                                        >  2:4438972/1‑150 (MQ=255)
                                                                                                            aaGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAAcc                                       <  1:4737651/150‑1 (MQ=255)
                                                                                                            aaGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAAcc                                       <  2:4074479/150‑1 (MQ=255)
                                                                                                            aaGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAAcc                                       <  1:3479249/150‑1 (MQ=255)
                                                                                                              gACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGt                                     <  2:4727553/150‑1 (MQ=255)
                                                                                                               aCTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTg                                    <  2:694322/150‑1 (MQ=255)
                                                                                                                cTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGTTGCTGCTGCAGTCCATAGTACAAGCGTTACTTGAAGAAGGTCGCATCACCTAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGa                                   <  1:4129679/150‑1 (MQ=255)
                                                                                                                cTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGa                                   <  1:3291228/150‑1 (MQ=255)
                                                                                                                  cGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAg                                 <  1:2580079/150‑1 (MQ=255)
                                                                                                                  cGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAg                                 >  1:292734/1‑150 (MQ=255)
                                                                                                                               cAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTctct                    >  1:2423806/1‑150 (MQ=255)
                                                                                                                                 aGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTga                  <  1:4022495/150‑1 (MQ=255)
                                                                                                                                   tATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTgaga                >  2:4036544/1‑150 (MQ=255)
                                                                                                                                     tGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATg              <  2:1735940/150‑1 (MQ=255)
                                                                                                                                      ggAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGt             <  2:2917299/150‑1 (MQ=255)
                                                                                                                                      ggAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGt             <  1:1602148/150‑1 (MQ=255)
                                                                                                                                           aGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCa        >  2:1361748/1‑150 (MQ=255)
                                                                                                                                                aTTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCgg   >  1:2234238/1‑150 (MQ=255)
                                                                                                                                                 ttCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCggg  <  2:3910045/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AACCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGG  >  CP009273/3049073‑3049367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGG  >  CP009273/3049075‑3049367
                                                                                                                                                 |                                                                                                                                                   
CCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTATGCGGCAAGTATGGAACAGCGTATTAGGAT                                                                                                                                                 <  SRR19973063.2881337/150‑1 (MQ=60)
CCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGAT                                                                                                                                                 >  SRR19973063.4742621/1‑150 (MQ=60)
CCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGAT                                                                                                                                                 <  SRR19973063.2461183/150‑1 (MQ=60)
  AACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGACCAGCGTATTAGGATGC                                                                                                                                               >  SRR19973063.63613/1‑150 (MQ=60)
  AACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGC                                                                                                                                               <  SRR19973063.4300866/150‑1 (MQ=60)
    CGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTG                                                                                                                                             >  SRR19973063.3288529/1‑150 (MQ=60)
       TTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGTACAGCGTATTAGGATGCTGCAG                                                                                                                                          >  SRR19973063.2972829/1‑150 (MQ=60)
         GCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCA                                                                                                                                        <  SRR19973063.3630276/150‑1 (MQ=60)
         GCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCA                                                                                                                                        >  SRR19973063.3240314/1‑150 (MQ=60)
         GCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCA                                                                                                                                        >  SRR19973063.3240365/1‑150 (MQ=60)
           AAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGA                                                                                                                                      >  SRR19973063.287764/1‑150 (MQ=60)
           AAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGATGCAGCAGA                                                                                                                                      >  SRR19973063.3144745/1‑150 (MQ=60)
            AGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGAC                                                                                                                                     <  SRR19973063.4235201/150‑1 (MQ=60)
               TCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCAT                                                                                                                                  >  SRR19973063.3630611/1‑150 (MQ=60)
                 TGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAG                                                                                                                                >  SRR19973063.3922511/1‑150 (MQ=60)
                  GAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGA                                                                                                                               >  SRR19973063.2093944/1‑150 (MQ=60)
                  GAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGA                                                                                                                               >  SRR19973063.2091139/1‑150 (MQ=60)
                   AAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAA                                                                                                                              >  SRR19973063.1065964/1‑150 (MQ=60)
                   AAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAA                                                                                                                              >  SRR19973063.4676452/1‑150 (MQ=60)
                   AAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAA                                                                                                                              >  SRR19973063.4510289/1‑150 (MQ=60)
                    AAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAAC                                                                                                                             >  SRR19973063.1235103/1‑150 (MQ=60)
                    AAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAAC                                                                                                                             >  SRR19973063.1507681/1‑150 (MQ=60)
                     AGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACA                                                                                                                            <  SRR19973063.2770185/150‑1 (MQ=60)
                      GAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAA                                                                                                                           >  SRR19973063.4882390/1‑150 (MQ=60)
                       AACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAG                                                                                                                          <  SRR19973063.2946506/150‑1 (MQ=60)
                         CGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCG                                                                                                                        >  SRR19973063.927504/1‑150 (MQ=60)
                           CACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCATACCATATAACAAGCGTT                                                                                                                      >  SRR19973063.2115366/1‑150 (MQ=60)
                           CACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTT                                                                                                                      >  SRR19973063.552215/1‑150 (MQ=60)
                             CTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTAC                                                                                                                    <  SRR19973063.1360843/150‑1 (MQ=60)
                              TAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACT                                                                                                                   >  SRR19973063.675501/1‑150 (MQ=60)
                                GAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTG                                                                                                                 <  SRR19973063.1383656/150‑1 (MQ=60)
                                GAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTG                                                                                                                 >  SRR19973063.2566487/1‑150 (MQ=60)
                                    CACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACA                                                                                                             <  SRR19973063.3454641/150‑1 (MQ=60)
                                    CACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACA                                                                                                             <  SRR19973063.1732202/150‑1 (MQ=60)
                                       AAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGG                                                                                                          >  SRR19973063.823984/1‑150 (MQ=60)
                                          TACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTGGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCG                                                                                                       <  SRR19973063.3348898/150‑1 (MQ=60)
                                            CTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCA                                                                                                     >  SRR19973063.1711849/1‑150 (MQ=60)
                                            CTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCA                                                                                                     >  SRR19973063.1711894/1‑150 (MQ=60)
                                              GAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATC                                                                                                   <  SRR19973063.1342163/150‑1 (MQ=60)
                                               AACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCA                                                                                                  >  SRR19973063.4715435/1‑150 (MQ=60)
                                               AACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCA                                                                                                  <  SRR19973063.4022760/150‑1 (MQ=60)
                                                 CAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACC                                                                                                <  SRR19973063.2582405/150‑1 (MQ=60)
                                                  AGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCG                                                                                               >  SRR19973063.1410683/1‑150 (MQ=60)
                                                   GTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGA                                                                                              >  SRR19973063.1672225/1‑150 (MQ=60)
                                                    TTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAA                                                                                             <  SRR19973063.3832434/150‑1 (MQ=60)
                                                     TGGTCTTCATTGCCTCTTTGTATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGTCAAGTATTGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAA                                                                                            <  SRR19973063.2218795/150‑1 (MQ=60)
                                                     TGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAA                                                                                            <  SRR19973063.2218853/150‑1 (MQ=60)
                                                     TGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAA                                                                                            >  SRR19973063.210366/1‑150 (MQ=60)
                                                     TGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAA                                                                                            >  SRR19973063.2733091/1‑150 (MQ=60)
                                                        TCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAA                                                                                         >  SRR19973063.4624003/1‑150 (MQ=60)
                                                        TCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAA                                                                                         >  SRR19973063.743716/1‑150 (MQ=60)
                                                        TCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGAATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAA                                                                                         >  SRR19973063.4606269/1‑150 (MQ=60)
                                                           TCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTA                                                                                      <  SRR19973063.159239/150‑1 (MQ=60)
                                                             ATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAAC                                                                                    >  SRR19973063.5151406/1‑150 (MQ=60)
                                                             ATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAAC                                                                                    >  SRR19973063.2196580/1‑150 (MQ=60)
                                                             ATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAAC                                                                                    >  SRR19973063.2185195/1‑150 (MQ=60)
                                                                GCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAA                                                                                 <  SRR19973063.3876914/150‑1 (MQ=60)
                                                                  CGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAA                                                                               >  SRR19973063.1176858/1‑150 (MQ=60)
                                                                    CATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACT                                                                             <  SRR19973063.4425172/150‑1 (MQ=60)
                                                                     ATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTT                                                                            >  SRR19973063.5143998/1‑150 (MQ=60)
                                                                         AATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAA                                                                        <  SRR19973063.771470/150‑1 (MQ=60)
                                                                           TATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATG                                                                      >  SRR19973063.4739647/1‑150 (MQ=60)
                                                                           TATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATG                                                                      >  SRR19973063.680564/1‑150 (MQ=60)
                                                                            ATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGA                                                                     >  SRR19973063.3460741/1‑150 (MQ=60)
                                                                                 CTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTT                                                                <  SRR19973063.2004928/150‑1 (MQ=60)
                                                                                 CTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTT                                                                <  SRR19973063.2005463/150‑1 (MQ=60)
                                                                                  TATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTT                                                               <  SRR19973063.1725494/150‑1 (MQ=60)
                                                                                   ATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTT                                                              >  SRR19973063.4740997/1‑150 (MQ=60)
                                                                                   ATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTT                                                              <  SRR19973063.2349234/150‑1 (MQ=60)
                                                                                   ATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTT                                                              >  SRR19973063.3910775/1‑150 (MQ=60)
                                                                                    TGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTC                                                             <  SRR19973063.3200910/150‑1 (MQ=60)
                                                                                     GAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTTCGCTGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCT                                                            <  SRR19973063.4631878/150‑1 (MQ=60)
                                                                                     GAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCG                                                            >  SRR19973063.1877275/1‑150 (MQ=60)
                                                                                      AGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGG                                                           >  SRR19973063.690516/1‑150 (MQ=60)
                                                                                      AGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGG                                                           >  SRR19973063.690455/1‑150 (MQ=60)
                                                                                      AGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGG                                                           >  SRR19973063.4876894/1‑150 (MQ=60)
                                                                                       GTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGT                                                          >  SRR19973063.563177/1‑150 (MQ=60)
                                                                                         TAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGTAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTT                                                        <  SRR19973063.2047404/150‑1 (MQ=60)
                                                                                         TAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTT                                                        <  SRR19973063.469022/150‑1 (MQ=60)
                                                                                         TAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTT                                                        <  SRR19973063.4444168/150‑1 (MQ=60)
                                                                                          AGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTA                                                       >  SRR19973063.4721008/1‑150 (MQ=60)
                                                                                            CGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACC                                                     <  SRR19973063.3837322/150‑1 (MQ=60)
                                                                                             GCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTG                                                    <  SRR19973063.2737299/150‑1 (MQ=60)
                                                                                              CAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGT                                                   >  SRR19973063.1887949/1‑150 (MQ=60)
                                                                                                ATAAAAAGCAAAGACTCGTGACTACTCGGCAAGTATTGAACAGCGTATTAGGATGCTGCAGCAGAAAATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACAAAAACTTTGAATGACACTTTTCTGTTTAATGTGT                                                 >  SRR19973063.1635525/1‑150 (MQ=60)
                                                                                                 GAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGT                                                <  SRR19973063.3128863/150‑1 (MQ=60)
                                                                                                  AAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTA                                               >  SRR19973063.4601995/1‑150 (MQ=60)
                                                                                                  AAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTA                                               >  SRR19973063.3560172/1‑150 (MQ=60)
                                                                                                   AAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAG                                              >  SRR19973063.519236/1‑150 (MQ=60)
                                                                                                   AAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAG                                              >  SRR19973063.87075/1‑150 (MQ=60)
                                                                                                   AAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAG                                              >  SRR19973063.981075/1‑150 (MQ=60)
                                                                                                      AGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGT                                           >  SRR19973063.1174493/1‑150 (MQ=60)
                                                                                                      AGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGT                                           >  SRR19973063.3763703/1‑150 (MQ=60)
                                                                                                          AAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACC                                       <  SRR19973063.4752744/150‑1 (MQ=60)
                                                                                                          AAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACC                                       <  SRR19973063.3490345/150‑1 (MQ=60)
                                                                                                          AAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACC                                       <  SRR19973063.3490372/150‑1 (MQ=60)
                                                                                                            GACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGT                                     >  SRR19973063.473705/1‑150 (MQ=60)
                                                                                                              CTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGTTGCTGCTGCAGTCCATAGTACAAGCGTTACTTGAAGAAGGTCGCATCACCTAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGA                                   <  SRR19973063.4142896/150‑1 (MQ=60)
                                                                                                              CTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGA                                   <  SRR19973063.4142919/150‑1 (MQ=60)
                                                                                                              CTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGA                                   <  SRR19973063.3301768/150‑1 (MQ=60)
                                                                                                              CTCGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGA                                   >  SRR19973063.1483920/1‑150 (MQ=60)
                                                                                                                CGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAG                                 >  SRR19973063.293600/1‑150 (MQ=60)
                                                                                                                CGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAG                                 >  SRR19973063.2308747/1‑150 (MQ=60)
                                                                                                                CGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAG                                 <  SRR19973063.608816/150‑1 (MQ=60)
                                                                                                                CGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAG                                 <  SRR19973063.2588283/150‑1 (MQ=60)
                                                                                                                CGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAG                                 <  SRR19973063.2588218/150‑1 (MQ=60)
                                                                                                                CGTGACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAG                                 <  SRR19973063.2588174/150‑1 (MQ=60)
                                                                                                                   GACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACA                              >  SRR19973063.437071/1‑150 (MQ=60)
                                                                                                                   GACTACGCGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACA                              >  SRR19973063.1436501/1‑150 (MQ=60)
                                                                                                                          CGGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTC                       <  SRR19973063.4525243/150‑1 (MQ=60)
                                                                                                                           GGCAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCT                      >  SRR19973063.3522741/1‑150 (MQ=60)
                                                                                                                             CAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCT                    >  SRR19973063.2431540/1‑150 (MQ=60)
                                                                                                                             CAAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCT                    >  SRR19973063.2431554/1‑150 (MQ=60)
                                                                                                                              AAGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTG                   >  SRR19973063.4203247/1‑150 (MQ=60)
                                                                                                                               AGTATGGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGA                  <  SRR19973063.4035398/150‑1 (MQ=60)
                                                                                                                                    GGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGT             >  SRR19973063.834156/1‑150 (MQ=60)
                                                                                                                                    GGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGT             <  SRR19973063.1607057/150‑1 (MQ=60)
                                                                                                                                    GGAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGT             <  SRR19973063.1606836/150‑1 (MQ=60)
                                                                                                                                     GAACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTT            <  SRR19973063.3584650/150‑1 (MQ=60)
                                                                                                                                      AACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTC           >  SRR19973063.4064514/1‑150 (MQ=60)
                                                                                                                                       ACAGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCG          >  SRR19973063.2881303/1‑150 (MQ=60)
                                                                                                                                         AGCGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCA        <  SRR19973063.2837179/150‑1 (MQ=60)
                                                                                                                                           CGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAG      >  SRR19973063.3062983/1‑150 (MQ=60)
                                                                                                                                           CGTATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAG      <  SRR19973063.1945019/150‑1 (MQ=60)
                                                                                                                                              ATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGG   >  SRR19973063.2241369/1‑150 (MQ=60)
                                                                                                                                              ATTAGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGG   >  SRR19973063.2241324/1‑150 (MQ=60)
                                                                                                                                               TTAGGATGCTGCAGCAGACCATAGAACACGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGG  <  SRR19973063.2338590/150‑1 (MQ=60)
                                                                                                                                                 |                                                                                                                                                   
CCAACGGTTGCAAGATCTGAAAGAACGCACTAGAGTCACAAATACTGAACAGTTGGTCTTCATTGCCGCATTGAATATCAGCTATGAGTTAGCGCAAGAAAAAGCAAAGACTCGTGACTACGCGGCAAGTATGGAACAGCGTATTCGGATGCTGCAGCAGACCATAGAACAAGCGTTACTTGAACAAGGTCGCATCACCGAAAAAACTAACCAAAACTTTGAATGACACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGG  >  CP009273/3049075‑3049367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑