Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,768,689 C→A 100% S215* (TCA→TAA)  ydiB → quinate/shikimate 5‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,768,6890CA95.2% 605.5 / 19.3 207S215* (TCA→TAA) ydiBquinate/shikimate 5‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (2/8);  new base A (103/94);  total (105/102)
Fisher's exact test for biased strand distribution p-value = 5.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 210 total aligned reads displayed.

TTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGG  >  CP009273/1768544‑1768833
                                                                                                                                                 |                                                                                                                                                
ttCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGaataat                                                                                                                                                <  1:2710006/148‑1 (MQ=255)
  cgcgCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTg                                                                                                                                              <  1:170583/148‑1 (MQ=255)
        gCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAAt                                                                                                                                        <  1:10374/148‑1 (MQ=255)
         cGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATg                                                                                                                                       <  1:2386784/148‑1 (MQ=255)
         cGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATg                                                                                                                                       >  1:3259463/1‑148 (MQ=255)
         cGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATg                                                                                                                                       >  1:735178/1‑148 (MQ=255)
            gTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGata                                                                                                                                    <  1:1295046/148‑1 (MQ=255)
              tAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATc                                                                                                                                  >  2:1649415/1‑148 (MQ=255)
              tAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATc                                                                                                                                  <  1:2264912/148‑1 (MQ=255)
                 tGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGt                                                                                                                               <  1:2484335/148‑1 (MQ=255)
                 tGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGt                                                                                                                               <  2:1911837/148‑1 (MQ=255)
                 tGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGt                                                                                                                               <  1:2567455/148‑1 (MQ=255)
                  gAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTc                                                                                                                              >  2:2113253/1‑148 (MQ=255)
                  gAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTc                                                                                                                              >  2:601819/1‑148 (MQ=255)
                     aaCACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGt                                                                                                                           <  2:2756427/148‑1 (MQ=255)
                      acacCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGtt                                                                                                                          <  1:2765842/148‑1 (MQ=255)
                       cacCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTa                                                                                                                         >  1:210684/1‑148 (MQ=255)
                       cacCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTa                                                                                                                         <  1:2340291/148‑1 (MQ=255)
                             ttGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCg                                                                                                                   >  2:3240466/1‑148 (MQ=255)
                             ttGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCg                                                                                                                   <  1:1085478/148‑1 (MQ=255)
                             ttGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCg                                                                                                                   >  1:1342348/1‑148 (MQ=255)
                             ttGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCg                                                                                                                   >  1:1338592/1‑148 (MQ=255)
                             ttGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCg                                                                                                                   >  2:1468515/1‑148 (MQ=255)
                             gtgtgTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCg                                                                                                                   <  2:2276830/147‑1 (MQ=255)
                              tgtgTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCgg                                                                                                                  <  2:2008083/148‑1 (MQ=255)
                              tgtgTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCgg                                                                                                                  >  2:1560554/1‑148 (MQ=255)
                                tgtCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGa                                                                                                                <  1:2587462/148‑1 (MQ=255)
                                 gtcgtcACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGAc                                                                                                               >  2:1723736/1‑148 (MQ=255)
                                   cgtcACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACtt                                                                                                             <  2:3081713/148‑1 (MQ=255)
                                      cACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTg                                                                                                          <  2:1529961/148‑1 (MQ=255)
                                      cACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTg                                                                                                          >  2:785602/1‑148 (MQ=255)
                                       aCGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTgg                                                                                                         <  1:2128634/148‑1 (MQ=255)
                                       aCGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTgg                                                                                                         <  1:2647365/148‑1 (MQ=255)
                                       aCGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTgg                                                                                                         <  1:2128691/148‑1 (MQ=255)
                                          gTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACTTTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCa                                                                                                      <  2:2843793/148‑1 (MQ=255)
                                           tCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCAc                                                                                                     <  2:1007831/148‑1 (MQ=255)
                                           tCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCAc                                                                                                     >  1:1552518/1‑148 (MQ=255)
                                            cACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACt                                                                                                    >  1:1561145/1‑148 (MQ=255)
                                             aCCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTg                                                                                                   >  2:1641254/1‑148 (MQ=255)
                                                  tctcGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGc                                                                                              >  1:348666/1‑148 (MQ=255)
                                                   ctcGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCg                                                                                             <  2:1342348/148‑1 (MQ=255)
                                                    tcGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCgt                                                                                            <  2:646534/148‑1 (MQ=255)
                                                      gCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCgtgt                                                                                          >  1:1655048/1‑148 (MQ=255)
                                                       ccGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGta                                                                                         >  1:814184/1‑148 (MQ=255)
                                                          aTCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATaa                                                                                      <  2:84752/148‑1 (MQ=255)
                                                           tCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAAc                                                                                     <  1:269440/148‑1 (MQ=255)
                                                            cAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAAcc                                                                                    <  2:2147029/148‑1 (MQ=255)
                                                              gCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCg                                                                                  >  2:25678/1‑148 (MQ=255)
                                                              gCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACAATTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCg                                                                                  >  1:643511/1‑148 (MQ=255)
                                                               cAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGc                                                                                 >  2:1893005/1‑148 (MQ=255)
                                                               cAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGc                                                                                 >  2:1102645/1‑148 (MQ=255)
                                                                 aGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCat                                                                               <  1:1629271/148‑1 (MQ=255)
                                                                 aGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATACGGGACTTCTGGTCACTGAATGCTTGTATAACCCGCat                                                                               >  2:713039/1‑148 (MQ=255)
                                                                   ccTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCatat                                                                             >  1:3192957/1‑148 (MQ=255)
                                                                    cTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATg                                                                            <  1:678587/148‑1 (MQ=255)
                                                                     tttGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGa                                                                           >  2:457927/1‑148 (MQ=255)
                                                                      ttGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGAc                                                                          >  1:3556618/1‑148 (MQ=255)
                                                                      ttGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGAc                                                                          >  1:2978266/1‑148 (MQ=255)
                                                                        gCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGa                                                                        <  2:122423/148‑1 (MQ=255)
                                                                         cTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGaa                                                                       <  1:2365546/148‑1 (MQ=255)
                                                                            aaGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGtt                                                                    <  1:744999/148‑1 (MQ=255)
                                                                              gCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTAt                                                                  >  2:2528117/1‑148 (MQ=255)
                                                                                ccTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTg                                                                <  1:2849446/148‑1 (MQ=255)
                                                                                ccTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTg                                                                <  1:2849334/148‑1 (MQ=255)
                                                                                  tGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTgca                                                              >  1:3418988/1‑148 (MQ=255)
                                                                                   ggCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTgcag                                                             >  2:230448/1‑148 (MQ=255)
                                                                                           ccgACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGca                                                     <  1:583673/148‑1 (MQ=255)
                                                                                           ccgACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGca                                                     <  2:901630/148‑1 (MQ=255)
                                                                                            cgACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGcaa                                                    >  2:1485150/1‑148 (MQ=255)
                                                                                                aTTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAg                                                <  1:88440/148‑1 (MQ=255)
                                                                                                 ttttAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGc                                               <  1:1944797/148‑1 (MQ=255)
                                                                                                  tttAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCt                                              >  2:3523011/1‑148 (MQ=255)
                                                                                                  tttAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCt                                              >  2:3522935/1‑148 (MQ=255)
                                                                                                       ccAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTg                                         >  2:3264369/1‑148 (MQ=255)
                                                                                                        cAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGc                                        >  1:2194067/1‑148 (MQ=255)
                                                                                                        cAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGc                                        >  1:318056/1‑148 (MQ=255)
                                                                                                             gCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAAc                                   >  1:2330971/1‑148 (MQ=255)
                                                                                                              cacaAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACg                                  <  1:958290/148‑1 (MQ=255)
                                                                                                              cacaAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACg                                  <  1:2874025/148‑1 (MQ=255)
                                                                                                               acaAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGa                                 <  2:2461382/148‑1 (MQ=255)
                                                                                                                   aaGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGa                             <  2:943784/148‑1 (MQ=255)
                                                                                                                   aaGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGa                             >  2:2504107/1‑148 (MQ=255)
                                                                                                                        ggTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGAt                        <  2:735178/148‑1 (MQ=255)
                                                                                                                            tGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACgg                    >  1:1478740/1‑148 (MQ=255)
                                                                                                                              aaaCCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCa                  >  2:3476205/1‑148 (MQ=255)
                                                                                                                                aCCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtg                <  2:3032398/148‑1 (MQ=255)
                                                                                                                                 ccccTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgt               >  2:3003132/1‑148 (MQ=255)
                                                                                                                                 ccccTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgt               >  2:676181/1‑148 (MQ=255)
                                                                                                                                  cccTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgtt              >  1:1843/1‑148 (MQ=255)
                                                                                                                                  cccTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgtt              >  1:1230025/1‑148 (MQ=255)
                                                                                                                                   ccTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttg             <  1:1168683/148‑1 (MQ=255)
                                                                                                                                     ttGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttgtt           >  2:2360497/1‑148 (MQ=255)
                                                                                                                                     ttGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttgtt           <  2:3150552/148‑1 (MQ=255)
                                                                                                                                      tGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCACAACGATTGATGGATACGGCAtgttgttg          >  1:2649761/1‑148 (MQ=255)
                                                                                                                                        agaATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTtgtg        >  2:1816219/1‑148 (MQ=255)
                                                                                                                                         gaatgaatAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTgg       <  2:2771571/148‑1 (MQ=255)
                                                                                                                                            tgaatAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCaa    <  1:25678/148‑1 (MQ=255)
                                                                                                                                            tgaatAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCaa    >  2:2599980/1‑148 (MQ=255)
                                                                                                                                            tgaatAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCaa    >  1:3515925/1‑148 (MQ=255)
                                                                                                                                              aataatTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAgg  <  2:2762268/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGG  >  CP009273/1768544‑1768833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCT  >  CP009273/1768540‑1768837
                                                                                                                                                     |                                                                                                                                                    
CGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATA                                                                                                                                                      <  SRR19973066.681479/150‑1 (MQ=60)
CGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATA                                                                                                                                                      <  SRR19973066.682029/150‑1 (MQ=60)
 GCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAA                                                                                                                                                     <  SRR19973066.712974/150‑1 (MQ=60)
   CTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATT                                                                                                                                                   <  SRR19973066.204837/150‑1 (MQ=60)
   CTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATT                                                                                                                                                   <  SRR19973066.3491686/150‑1 (MQ=60)
    TTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTG                                                                                                                                                  <  SRR19973066.2728200/150‑1 (MQ=60)
      CGCGCAGCTGGTTAATGAATACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCTAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGT                                                                                                                                                <  SRR19973066.3575449/150‑1 (MQ=60)
      CGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGT                                                                                                                                                <  SRR19973066.172108/150‑1 (MQ=60)
      CGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGT                                                                                                                                                >  SRR19973066.882342/1‑150 (MQ=60)
         GCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAA                                                                                                                                             >  SRR19973066.85598/1‑150 (MQ=60)
           AGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATG                                                                                                                                           >  SRR19973066.3280903/1‑150 (MQ=60)
           AGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATG                                                                                                                                           >  SRR19973066.740633/1‑150 (MQ=60)
            GCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGA                                                                                                                                          <  SRR19973066.10540/150‑1 (MQ=60)
             CGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGAT                                                                                                                                         <  SRR19973066.2402864/150‑1 (MQ=60)
             CGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATCAT                                                                                                                                         <  SRR19973066.2402804/150‑1 (MQ=60)
                GTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATC                                                                                                                                      <  SRR19973066.1304427/150‑1 (MQ=60)
                 TTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCA                                                                                                                                     <  SRR19973066.3275310/150‑1 (MQ=60)
                 TTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCA                                                                                                                                     <  SRR19973066.1617246/150‑1 (MQ=60)
                 TTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCA                                                                                                                                     <  SRR19973066.3398692/150‑1 (MQ=60)
                  TAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAG                                                                                                                                    <  SRR19973066.2280499/150‑1 (MQ=60)
                     TGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCT                                                                                                                                 <  SRR19973066.2501284/150‑1 (MQ=60)
                     TGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCT                                                                                                                                 <  SRR19973066.2584877/150‑1 (MQ=60)
                       AAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGT                                                                                                                               >  SRR19973066.1959060/1‑150 (MQ=60)
                        AAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTT                                                                                                                              <  SRR19973066.1870884/150‑1 (MQ=60)
                         AACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTA                                                                                                                             >  SRR19973066.212438/1‑150 (MQ=60)
                          ACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTAC                                                                                                                            <  SRR19973066.2784469/150‑1 (MQ=60)
                          ACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTAC                                                                                                                            <  SRR19973066.2784240/150‑1 (MQ=60)
                           CACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACA                                                                                                                           <  SRR19973066.2357142/150‑1 (MQ=60)
                           CACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACA                                                                                                                           <  SRR19973066.2356229/150‑1 (MQ=60)
                           CACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACA                                                                                                                           <  SRR19973066.3419549/150‑1 (MQ=60)
                               GATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCG                                                                                                                       >  SRR19973066.1348185/1‑150 (MQ=60)
                               GATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCG                                                                                                                       >  SRR19973066.1348305/1‑150 (MQ=60)
                               GATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCG                                                                                                                       >  SRR19973066.1348322/1‑150 (MQ=60)
                               GATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCG                                                                                                                       >  SRR19973066.1352089/1‑150 (MQ=60)
                                 TTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGG                                                                                                                     <  SRR19973066.1093400/150‑1 (MQ=60)
                                    TGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACT                                                                                                                  <  SRR19973066.2604989/150‑1 (MQ=60)
                                    TGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACT                                                                                                                  <  SRR19973066.2604999/150‑1 (MQ=60)
                                     GTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTT                                                                                                                 >  SRR19973066.2306832/1‑150 (MQ=60)
                                      TCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTC                                                                                                                <  SRR19973066.2960965/150‑1 (MQ=60)
                                           ACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTC                                                                                                           <  SRR19973066.2665779/150‑1 (MQ=60)
                                           ACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTC                                                                                                           <  SRR19973066.2665147/150‑1 (MQ=60)
                                           ACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTC                                                                                                           <  SRR19973066.2143381/150‑1 (MQ=60)
                                           ACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTC                                                                                                           <  SRR19973066.2143397/150‑1 (MQ=60)
                                           ACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTC                                                                                                           <  SRR19973066.2143438/150‑1 (MQ=60)
                                             GGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCAC                                                                                                         >  SRR19973066.1563655/1‑150 (MQ=60)
                                              GTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACT                                                                                                        >  SRR19973066.1572381/1‑150 (MQ=60)
                                              GTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACT                                                                                                        >  SRR19973066.1571902/1‑150 (MQ=60)
                                                 ACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAA                                                                                                     >  SRR19973066.2887165/1‑150 (MQ=60)
                                                  CCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAAT                                                                                                    <  SRR19973066.2523211/150‑1 (MQ=60)
                                                   CGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATG                                                                                                   >  SRR19973066.630810/1‑150 (MQ=60)
                                                    GATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGC                                                                                                  >  SRR19973066.351384/1‑150 (MQ=60)
                                                    GATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGC                                                                                                  >  SRR19973066.351971/1‑150 (MQ=60)
                                                       CTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTG                                                                                               >  SRR19973066.2823691/1‑150 (MQ=60)
                                                        TCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGT                                                                                              >  SRR19973066.1667106/1‑150 (MQ=60)
                                                        TCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGT                                                                                              <  SRR19973066.1711626/150‑1 (MQ=60)
                                                         CGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTA                                                                                             >  SRR19973066.820151/1‑150 (MQ=60)
                                                               TCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCTCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGTGACTTCTGGTCACTGAATGCGTGTATAACCC                                                                                       <  SRR19973066.271443/150‑1 (MQ=60)
                                                               TCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCC                                                                                       <  SRR19973066.271418/150‑1 (MQ=60)
                                                               TCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCC                                                                                       <  SRR19973066.271440/150‑1 (MQ=60)
                                                               TCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCC                                                                                       <  SRR19973066.1287477/150‑1 (MQ=60)
                                                                CAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACAATTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCG                                                                                      >  SRR19973066.648170/1‑150 (MQ=60)
                                                                CAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACAATTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCG                                                                                      >  SRR19973066.648178/1‑150 (MQ=60)
                                                                     AGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATAT                                                                                 >  SRR19973066.3214169/1‑150 (MQ=60)
                                                                     AGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATAT                                                                                 >  SRR19973066.1768537/1‑150 (MQ=60)
                                                                     AGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATAT                                                                                 <  SRR19973066.1641094/150‑1 (MQ=60)
                                                                      GCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATG                                                                                >  SRR19973066.950670/1‑150 (MQ=60)
                                                                      GCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATG                                                                                >  SRR19973066.2336629/1‑150 (MQ=60)
                                                                        CTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGAC                                                                              >  SRR19973066.2997944/1‑150 (MQ=60)
                                                                        CTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGAC                                                                              <  SRR19973066.683552/150‑1 (MQ=60)
                                                                        CTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGAC                                                                              >  SRR19973066.3580209/1‑150 (MQ=60)
                                                                             CTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGT                                                                         <  SRR19973066.2381562/150‑1 (MQ=60)
                                                                               GAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTA                                                                       <  SRR19973066.2480459/150‑1 (MQ=60)
                                                                                AAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTAT                                                                      <  SRR19973066.750529/150‑1 (MQ=60)
                                                                                AAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTAT                                                                      <  SRR19973066.750526/150‑1 (MQ=60)
                                                                                AAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTAT                                                                      >  SRR19973066.1558997/1‑150 (MQ=60)
                                                                                   CCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGC                                                                   >  SRR19973066.2139490/1‑150 (MQ=60)
                                                                                    CCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCA                                                                  <  SRR19973066.2868480/150‑1 (MQ=60)
                                                                                    CCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCA                                                                  <  SRR19973066.2868549/150‑1 (MQ=60)
                                                                                    CCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCA                                                                  <  SRR19973066.2868593/150‑1 (MQ=60)
                                                                                    CCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCA                                                                  >  SRR19973066.3441861/1‑150 (MQ=60)
                                                                                    CCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCA                                                                  >  SRR19973066.3442414/1‑150 (MQ=60)
                                                                                       GGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCA                                                               >  SRR19973066.1065817/1‑150 (MQ=60)
                                                                                               CCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAAC                                                       >  SRR19973066.2195034/1‑150 (MQ=60)
                                                                                               CCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAAC                                                       <  SRR19973066.587753/150‑1 (MQ=60)
                                                                                                CGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACA                                                      >  SRR19973066.2633877/1‑150 (MQ=60)
                                                                                                CGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACA                                                      >  SRR19973066.2691125/1‑150 (MQ=60)
                                                                                                    ATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCT                                                  <  SRR19973066.89400/150‑1 (MQ=60)
                                                                                                     TTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTG                                                 <  SRR19973066.1958575/150‑1 (MQ=60)
                                                                                                     TTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTG                                                 <  SRR19973066.1133372/150‑1 (MQ=60)
                                                                                                          ACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGC                                            >  SRR19973066.320440/1‑150 (MQ=60)
                                                                                                          ACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGC                                            >  SRR19973066.2209287/1‑150 (MQ=60)
                                                                                                            CAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAA                                          >  SRR19973066.2254825/1‑150 (MQ=60)
                                                                                                               TGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAAC                                       >  SRR19973066.2346960/1‑150 (MQ=60)
                                                                                                               TGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAAC                                       >  SRR19973066.2346878/1‑150 (MQ=60)
                                                                                                                  CACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGAT                                    >  SRR19973066.728348/1‑150 (MQ=60)
                                                                                                                  CACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGAT                                    <  SRR19973066.965310/150‑1 (MQ=60)
                                                                                                                  CACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGAT                                    <  SRR19973066.2893291/150‑1 (MQ=60)
                                                                                                                   ACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATT                                   <  SRR19973066.3035928/150‑1 (MQ=60)
                                                                                                                    CAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTG                                  <  SRR19973066.453620/150‑1 (MQ=60)
                                                                                                                         GTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGA                             <  SRR19973066.1638661/150‑1 (MQ=60)
                                                                                                                          TGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGAT                            >  SRR19973066.1449729/1‑150 (MQ=60)
                                                                                                                          TGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGAT                            >  SRR19973066.999059/1‑150 (MQ=60)
                                                                                                                          TGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGAT                            >  SRR19973066.754780/1‑150 (MQ=60)
                                                                                                                              TATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGG                        >  SRR19973066.1489140/1‑150 (MQ=60)
                                                                                                                                    ACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTT                  >  SRR19973066.1869/1‑150 (MQ=60)
                                                                                                                                    ACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTT                  <  SRR19973066.1661428/150‑1 (MQ=60)
                                                                                                                                    ACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTT                  >  SRR19973066.1238763/1‑150 (MQ=60)
                                                                                                                                       CCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTT               <  SRR19973066.1177530/150‑1 (MQ=60)
                                                                                                                                       CCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTT               <  SRR19973066.1176945/150‑1 (MQ=60)
                                                                                                                                        CTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCACAACGATTGATGGATACGGCATGTTGTTG              >  SRR19973066.2667548/1‑150 (MQ=60)
                                                                                                                                          TGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTG            <  SRR19973066.3207458/150‑1 (MQ=60)
                                                                                                                                              AATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAA        >  SRR19973066.3539339/1‑150 (MQ=60)
                                                                                                                                                TGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGG      <  SRR19973066.26044/150‑1 (MQ=60)
                                                                                                                                                TGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGG      <  SRR19973066.26046/150‑1 (MQ=60)
                                                                                                                                                   ATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGC   >  SRR19973066.2684009/1‑150 (MQ=60)
                                                                                                                                                   ATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGC   >  SRR19973066.3034236/1‑150 (MQ=60)
                                                                                                                                                    TAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCT  <  SRR19973066.2818528/150‑1 (MQ=60)
                                                                                                                                                    TAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCT  <  SRR19973066.2820349/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCT  >  CP009273/1768540‑1768837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑