Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F247 I0 R1	1496	299.8	9582018	97.8%	9371213	149.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,768,689	C→A	85.2%	S215* (TCA→TAA)	ydiB →	quinate/shikimate 5‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,768,689	0	C	A	85.2%	588.6 / 117.0	262	S215* (TCA→TAA)	ydiB	quinate/shikimate 5‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand): ref base C (21/18); new base A (96/127); total (117/145)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.02e-01

Only 100 of 266 total aligned reads displayed.

CTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGC  >  CP009273/1768543‑1768836

 ttCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAAtt                                                                                                                                                  <  2:422911/149‑1 (MQ=255)

  tCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTg                                                                                                                                                 <  2:3460390/149‑1 (MQ=255)

   cgcgCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTgg                                                                                                                                                <  1:1971505/149‑1 (MQ=255)

    gcgcAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGt                                                                                                                                               <  1:492491/149‑1 (MQ=255)

    gcgcAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGt                                                                                                                                               <  2:1724854/149‑1 (MQ=255)

      gcAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTa                                                                                                                                             <  1:2016474/149‑1 (MQ=255)

      gcAGCGGGTTAATGAAAACACCGATGGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTGTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAACGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTa                                                                                                                                             <  2:3914642/149‑1 (MQ=255)

        aGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAAt                                                                                                                                           <  2:3527713/149‑1 (MQ=255)

         gCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATg                                                                                                                                          <  2:1943722/149‑1 (MQ=255)

         gCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATg                                                                                                                                          <  1:2664500/149‑1 (MQ=255)

          cGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGa                                                                                                                                         <  2:1592128/149‑1 (MQ=255)

          cGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGa                                                                                                                                         <  1:1358620/149‑1 (MQ=255)

           gggTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGat                                                                                                                                        >  1:2357300/1‑149 (MQ=255)

            ggTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGata                                                                                                                                       >  1:4667502/1‑149 (MQ=255)

            ggTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGata                                                                                                                                       >  1:28351/1‑149 (MQ=255)

             gTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGatat                                                                                                                                      >  1:2784223/1‑149 (MQ=255)

               tAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCa                                                                                                                                    >  1:1513937/1‑149 (MQ=255)

               tAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCa                                                                                                                                    <  1:2804301/149‑1 (MQ=255)

                   gAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCt                                                                                                                                <  1:3570036/149‑1 (MQ=255)

                   gAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCt                                                                                                                                <  1:1135897/149‑1 (MQ=255)

                      aaCACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGtt                                                                                                                             >  2:2099136/1‑149 (MQ=255)

                        cacCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTAc                                                                                                                           >  2:130601/1‑149 (MQ=255)

                          ccGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACAt                                                                                                                         <  2:542853/149‑1 (MQ=255)

                             aTTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCg                                                                                                                      >  1:2413928/1‑149 (MQ=255)

                              ttGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCgg                                                                                                                     >  1:3899979/1‑149 (MQ=255)

                              ttGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCgg                                                                                                                     <  2:3993050/149‑1 (MQ=255)

                              ttGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAAGTGGTTAATGATATCAGTCTGTTACATCCgg                                                                                                                     >  2:3579869/1‑149 (MQ=255)

                               tgtgTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCggg                                                                                                                    >  2:3694860/1‑149 (MQ=255)

                                gtgtCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGa                                                                                                                   >  1:2314876/1‑149 (MQ=255)

                                   tcgtcACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACtt                                                                                                                <  1:1288073/149‑1 (MQ=255)

                                   tcgtcACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACtt                                                                                                                >  1:887315/1‑149 (MQ=255)

                                    cgtcACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTc                                                                                                               >  1:2403739/1‑149 (MQ=255)

                                      tcACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTg                                                                                                             >  1:3184883/1‑149 (MQ=255)

                                      tcACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTg                                                                                                             <  2:3546454/149‑1 (MQ=255)

                                           gTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCAc                                                                                                        >  1:1724126/1‑149 (MQ=255)

                                                 gATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATg                                                                                                  <  1:3458523/149‑1 (MQ=255)

                                                  aTCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGc                                                                                                 <  2:2833224/149‑1 (MQ=255)

                                                  aTCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACAGCCGGGACTTCTGGTCACTGAATGc                                                                                                 >  2:3177247/1‑149 (MQ=255)

                                                   tctcGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCg                                                                                                >  1:3308854/1‑149 (MQ=255)

                                                     tcGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCgtg                                                                                              <  2:1217846/149‑1 (MQ=255)

                                                        ccGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGtat                                                                                           <  2:1679978/149‑1 (MQ=255)

                                                         cGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGtata                                                                                          >  2:2363243/1‑149 (MQ=255)

                                                              aGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCg                                                                                     >  1:4119752/1‑149 (MQ=255)

                                                                cAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCa                                                                                   <  1:246974/149‑1 (MQ=255)

                                                                     cTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGACTGAATAATTGGTTAATGATCTCAGTCGGTTACATCCGGGACTGCTGGTCACTGAATGCGTGTATAACCCGCATATGa                                                                              >  2:4688086/1‑149 (MQ=255)

                                                                          cTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAg                                                                         <  2:3744880/149‑1 (MQ=255)

                                                                           tGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGt                                                                        <  1:3317033/149‑1 (MQ=255)

                                                                             aaGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTa                                                                      >  1:4421573/1‑149 (MQ=255)

                                                                                cccTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTg                                                                   <  1:191413/149‑1 (MQ=255)

                                                                                 ccTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTgc                                                                  >  2:1761539/1‑149 (MQ=255)

                                                                                 ccTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTgc                                                                  <  1:2107532/149‑1 (MQ=255)

                                                                                  cTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTgca                                                                 <  1:3112914/149‑1 (MQ=255)

                                                                                  cTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTgca                                                                 >  2:261979/1‑149 (MQ=255)

                                                                                  cTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTgca                                                                 <  1:1631350/149‑1 (MQ=255)

                                                                                        tCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGgc                                                           <  2:2509548/149‑1 (MQ=255)

                                                                                        tCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGgc                                                           <  2:2509431/149‑1 (MQ=255)

                                                                                        tCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGgc                                                           >  2:2395546/1‑149 (MQ=255)

                                                                                              gACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGcaaca                                                     >  1:1831818/1‑149 (MQ=255)

                                                                                               aCATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGcaacaa                                                    >  2:3384518/1‑149 (MQ=255)

                                                                                                cATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAg                                                   <  2:1591310/149‑1 (MQ=255)

                                                                                                 aTTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGc                                                  <  2:2458120/149‑1 (MQ=255)

                                                                                                  ttttAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCt                                                 >  2:2594129/1‑149 (MQ=255)

                                                                                                   tttAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTg                                                >  1:596026/1‑149 (MQ=255)

                                                                                                      aaCCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGtt                                             >  1:1819240/1‑149 (MQ=255)

                                                                                                      aaCCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGtt                                             >  1:3813552/1‑149 (MQ=255)

                                                                                                        ccAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGc                                           >  1:2849810/1‑149 (MQ=255)

                                                                                                           aTGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCaaa                                        >  2:4631739/1‑149 (MQ=255)

                                                                                                           aTGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCaaa                                        <  1:476163/149‑1 (MQ=255)

                                                                                                           aTGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCaaa                                        >  2:2185755/1‑149 (MQ=255)

                                                                                                           aTGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCaaa                                        >  2:2292539/1‑149 (MQ=255)

                                                                                                           aTGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATAGGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCaaa                                        >  2:440920/1‑149 (MQ=255)

                                                                                                               cacaAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGa                                    <  2:718623/149‑1 (MQ=255)

                                                                                                                acaAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGAt                                   >  1:4311047/1‑149 (MQ=255)

                                                                                                                  aaaaGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTg                                 >  2:252105/1‑149 (MQ=255)

                                                                                                                  aaaaGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTg                                 >  2:2750/1‑149 (MQ=255)

                                                                                                                    aaGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGAt                               >  1:2727261/1‑149 (MQ=255)

                                                                                                                    aaGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGAt                               <  2:4757075/149‑1 (MQ=255)

                                                                                                                    aaGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGAt                               <  2:1155612/149‑1 (MQ=255)

                                                                                                                      gTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATgg                             >  1:1198072/1‑149 (MQ=255)

                                                                                                                             tGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGc                      <  2:2900957/149‑1 (MQ=255)

                                                                                                                              gAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCa                     >  2:309325/1‑149 (MQ=255)

                                                                                                                              gAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCa                     >  2:1075326/1‑149 (MQ=255)

                                                                                                                               aaaCCCATTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAt                    <  2:43574/149‑1 (MQ=255)

                                                                                                                                 aCCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgt                  <  2:1115653/149‑1 (MQ=255)

                                                                                                                                 aCCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgt                  <  1:2248675/149‑1 (MQ=255)

                                                                                                                                  ccccTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgtt                 <  1:3694860/149‑1 (MQ=255)

                                                                                                                                    ccTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttgt               <  2:1494561/149‑1 (MQ=255)

                                                                                                                                     cTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttgtt              <  2:3482108/149‑1 (MQ=255)

                                                                                                                                      ttGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttgttg             >  2:146719/1‑149 (MQ=255)

                                                                                                                                         agaATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTgg          >  2:3837501/1‑149 (MQ=255)

                                                                                                                                         agaATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTgg          >  2:1415145/1‑149 (MQ=255)

                                                                                                                                          gaatgaatAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGc         >  1:4064059/1‑149 (MQ=255)

                                                                                                                                          gaatgaatAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGc         >  2:4604357/1‑149 (MQ=255)

                                                                                                                                          gaatgaatAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGc         >  2:3218169/1‑149 (MQ=255)

                                                                                                                                          gaatgaatAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGc         >  1:1672309/1‑149 (MQ=255)

                                                                                                                                           aatgaatAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCa        >  1:4269318/1‑149 (MQ=255)

                                                                                                                                            atgaatAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCaa       <  1:968827/149‑1 (MQ=255)

                                                                                                                                               aataatTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAggg    <  1:3953184/149‑1 (MQ=255)

                                                                                                                                               aataatTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAggg    >  2:3791635/1‑149 (MQ=255)

                                                                                                                                                 tCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGc  <  2:56178/149‑1 (MQ=255)

CTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGC  >  CP009273/1768543‑1768836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCT  >  CP009273/1768540‑1768837

CGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATA                                                                                                                                                      >  SRR19973071.3115492/1‑150 (MQ=60)

 GCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAA                                                                                                                                                     >  SRR19973071.1578621/1‑150 (MQ=60)

    TTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTG                                                                                                                                                  <  SRR19973071.4080833/150‑1 (MQ=60)

      CGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGT                                                                                                                                                <  SRR19973071.1978653/150‑1 (MQ=60)

      CGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGT                                                                                                                                                <  SRR19973071.480867/150‑1 (MQ=60)

      CGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGT                                                                                                                                                <  SRR19973071.3324911/150‑1 (MQ=60)

       GCGCAGCGGTTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCTCTCAGCAAGCCTTTGCTGTAGCCCTGGATTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTT                                                                                                                                               <  SRR19973071.495135/150‑1 (MQ=60)

       GCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTT                                                                                                                                               <  SRR19973071.494312/150‑1 (MQ=60)

        CGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTA                                                                                                                                              >  SRR19973071.2143567/1‑150 (MQ=60)

         GCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAA                                                                                                                                             <  SRR19973071.2023786/150‑1 (MQ=60)

            GCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGA                                                                                                                                          <  SRR19973071.2674055/150‑1 (MQ=60)

             CGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGAT                                                                                                                                         <  SRR19973071.1363633/150‑1 (MQ=60)

             CGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGAT                                                                                                                                         >  SRR19973071.2365904/1‑150 (MQ=60)

             CGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGAT                                                                                                                                         >  SRR19973071.2365840/1‑150 (MQ=60)

              GGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATA                                                                                                                                        >  SRR19973071.4684116/1‑150 (MQ=60)

              GGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATA                                                                                                                                        >  SRR19973071.4684205/1‑150 (MQ=60)

              GGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATA                                                                                                                                        >  SRR19973071.28534/1‑150 (MQ=60)

              GGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATA                                                                                                                                        >  SRR19973071.2430305/1‑150 (MQ=60)

               GGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATAT                                                                                                                                       >  SRR19973071.2794003/1‑150 (MQ=60)

                 TTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTGGAGAATGAATAATTGGTTAATGATATCA                                                                                                                                     >  SRR19973071.1519404/1‑150 (MQ=60)

                  TAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAG                                                                                                                                    <  SRR19973071.2814170/150‑1 (MQ=60)

                  TAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAG                                                                                                                                    <  SRR19973071.2814197/150‑1 (MQ=60)

                      GAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTG                                                                                                                                <  SRR19973071.3582463/150‑1 (MQ=60)

                      GAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTG                                                                                                                                <  SRR19973071.1185883/150‑1 (MQ=60)

                      GAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTG                                                                                                                                <  SRR19973071.1139869/150‑1 (MQ=60)

                         AACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTA                                                                                                                             >  SRR19973071.3970420/1‑150 (MQ=60)

                          ACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTAC                                                                                                                            >  SRR19973071.1761771/1‑150 (MQ=60)

                          ACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATCATATCAGTCTGTTAC                                                                                                                            >  SRR19973071.1761763/1‑150 (MQ=60)

                            ACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACAT                                                                                                                          <  SRR19973071.4589703/150‑1 (MQ=60)

                             CCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATC                                                                                                                         <  SRR19973071.2946700/150‑1 (MQ=60)

                             CCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATC                                                                                                                         <  SRR19973071.546749/150‑1 (MQ=60)

                               GATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCG                                                                                                                       >  SRR19973071.2422641/1‑150 (MQ=60)

                               GATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCG                                                                                                                       >  SRR19973071.3435134/1‑150 (MQ=60)

                                ATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGG                                                                                                                      >  SRR19973071.3913746/1‑150 (MQ=60)

                                  TGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGA                                                                                                                    >  SRR19973071.1242980/1‑150 (MQ=60)

                                  TGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGA                                                                                                                    >  SRR19973071.2053408/1‑150 (MQ=60)

                                  TGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGA                                                                                                                    >  SRR19973071.2323316/1‑150 (MQ=60)

                                   GTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGAC                                                                                                                   >  SRR19973071.3945231/1‑150 (MQ=60)

                                     GTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTT                                                                                                                 >  SRR19973071.2788692/1‑150 (MQ=60)

                                     GTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTT                                                                                                                 >  SRR19973071.2788662/1‑150 (MQ=60)

                                     GTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTT                                                                                                                 >  SRR19973071.890504/1‑150 (MQ=60)

                                      TCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTC                                                                                                                <  SRR19973071.1292760/150‑1 (MQ=60)

                                      TCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTC                                                                                                                <  SRR19973071.3690903/150‑1 (MQ=60)

                                      TCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTC                                                                                                                >  SRR19973071.2412401/1‑150 (MQ=60)

                                       CGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCT                                                                                                               <  SRR19973071.1624481/150‑1 (MQ=60)

                                        GTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTG                                                                                                              <  SRR19973071.227759/150‑1 (MQ=60)

                                        GTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTG                                                                                                              >  SRR19973071.3195966/1‑150 (MQ=60)

                                        GTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTG                                                                                                              >  SRR19973071.4432425/1‑150 (MQ=60)

                                             GGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCAC                                                                                                         >  SRR19973071.1730546/1‑150 (MQ=60)

                                             GGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCAC                                                                                                         >  SRR19973071.1955457/1‑150 (MQ=60)

                                              GTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACT                                                                                                        <  SRR19973071.2411719/150‑1 (MQ=60)

                                                CACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGA                                                                                                      <  SRR19973071.917777/150‑1 (MQ=60)

                                                    GATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGC                                                                                                  <  SRR19973071.3470725/150‑1 (MQ=60)

                                                    GATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGC                                                                                                  <  SRR19973071.639945/150‑1 (MQ=60)

                                                    GATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCATTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGC                                                                                                  <  SRR19973071.4078535/150‑1 (MQ=60)

                                                     ATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCG                                                                                                 >  SRR19973071.3320583/1‑150 (MQ=60)

                                                     ATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCG                                                                                                 <  SRR19973071.4293245/150‑1 (MQ=60)

                                                      TCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGT                                                                                                <  SRR19973071.3324636/150‑1 (MQ=60)

                                                       CTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTG                                                                                               >  SRR19973071.328903/1‑150 (MQ=60)

                                                        TCGCCGATCAGCAAGCCATTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGT                                                                                              <  SRR19973071.3863222/150‑1 (MQ=60)

                                                         CGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTA                                                                                             >  SRR19973071.2808268/1‑150 (MQ=60)

                                                             GATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAAC                                                                                         >  SRR19973071.1665853/1‑150 (MQ=60)

                                                              ATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACC                                                                                        <  SRR19973071.3668971/150‑1 (MQ=60)

                                                                CAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCG                                                                                      >  SRR19973071.4134414/1‑150 (MQ=60)

                                                                 AGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGC                                                                                     >  SRR19973071.2812563/1‑150 (MQ=60)

                                                                   CAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCAT                                                                                   <  SRR19973071.247912/150‑1 (MQ=60)

                                                                     AGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATAT                                                                                 <  SRR19973071.186097/150‑1 (MQ=60)

                                                                        CTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGAC                                                                              >  SRR19973071.1367545/1‑150 (MQ=60)

                                                                         TTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACG                                                                             <  SRR19973071.3207388/150‑1 (MQ=60)

                                                                           TGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAA                                                                           >  SRR19973071.922457/1‑150 (MQ=60)

                                                                              TGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTT                                                                        <  SRR19973071.3328795/150‑1 (MQ=60)

                                                                               GAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTA                                                                       >  SRR19973071.4437164/1‑150 (MQ=60)

                                                                                AAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTAT                                                                      >  SRR19973071.3959459/1‑150 (MQ=60)

                                                                                AAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTAT                                                                      <  SRR19973071.2579323/150‑1 (MQ=60)

                                                                                   CCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGC                                                                   <  SRR19973071.192206/150‑1 (MQ=60)

                                                                                   CCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGC                                                                   <  SRR19973071.191864/150‑1 (MQ=60)

                                                                                    CCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCA                                                                  <  SRR19973071.2115310/150‑1 (MQ=60)

                                                                                     CTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAG                                                                 <  SRR19973071.3123829/150‑1 (MQ=60)

                                                                                     CTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAG                                                                 <  SRR19973071.3123871/150‑1 (MQ=60)

                                                                                     CTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAG                                                                 <  SRR19973071.3124582/150‑1 (MQ=60)

                                                                                     CTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAG                                                                 <  SRR19973071.1637236/150‑1 (MQ=60)

                                                                                         CGTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGG                                                             <  SRR19973071.2452096/150‑1 (MQ=60)

                                                                                                CGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACA                                                      >  SRR19973071.1838625/1‑150 (MQ=60)

                                                                                                    ATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTAAATCCTGGACTTCTGCACACTCAATGCGAGTATAACCCGCATAGGACGAAGTTATTGCAGCAGGCGCAACAAGCT                                                  >  SRR19973071.1399039/1‑150 (MQ=60)

                                                                                                     TTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTG                                                 >  SRR19973071.598244/1‑150 (MQ=60)

                                                                                                        TAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTT                                              >  SRR19973071.1826013/1‑150 (MQ=60)

                                                                                                        TAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTT                                              <  SRR19973071.2354945/150‑1 (MQ=60)

                                                                                                        TAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTT                                              >  SRR19973071.2859308/1‑150 (MQ=60)

                                                                                                        TAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTT                                              >  SRR19973071.3827086/1‑150 (MQ=60)

                                                                                                        TAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTT                                              <  SRR19973071.1559096/150‑1 (MQ=60)

                                                                                                         AACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTG                                             <  SRR19973071.489836/150‑1 (MQ=60)

                                                                                                         AACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTG                                             >  SRR19973071.4485904/1‑150 (MQ=60)

                                                                                                          ACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGC                                            >  SRR19973071.2859942/1‑150 (MQ=60)

                                                                                                              ATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAA                                        <  SRR19973071.477907/150‑1 (MQ=60)

                                                                                                               TGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTAGAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAAC                                       <  SRR19973071.2140932/150‑1 (MQ=60)

                                                                                                                GGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACG                                      <  SRR19973071.1330975/150‑1 (MQ=60)

                                                                                                                  CACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGAT                                    >  SRR19973071.4326292/1‑150 (MQ=60)

                                                                                                                  CACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGAT                                    <  SRR19973071.207970/150‑1 (MQ=60)

                                                                                                                   ACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATT                                   <  SRR19973071.3496315/150‑1 (MQ=60)

                                                                                                                   ACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATT                                   <  SRR19973071.183070/150‑1 (MQ=60)

                                                                                                                    CAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTG                                  <  SRR19973071.2339945/150‑1 (MQ=60)

                                                                                                                      AAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGAT                                >  SRR19973071.2736957/1‑150 (MQ=60)

                                                                                                                        AGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGG                              >  SRR19973071.1202161/1‑150 (MQ=60)

                                                                                                                          TGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGAT                            >  SRR19973071.241542/1‑150 (MQ=60)

                                                                                                                            GGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATAC                          >  SRR19973071.2845330/1‑150 (MQ=60)

                                                                                                                              TATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGG                        >  SRR19973071.1721787/1‑150 (MQ=60)

                                                                                                                              TATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGG                        >  SRR19973071.1721249/1‑150 (MQ=60)

                                                                                                                              TATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGG                        >  SRR19973071.3756069/1‑150 (MQ=60)

                                                                                                                                   AACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGT                   >  SRR19973071.1924384/1‑150 (MQ=60)

                                                                                                                                    ACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTT                  <  SRR19973071.2256965/150‑1 (MQ=60)

                                                                                                                                     CCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTG                 <  SRR19973071.3707937/150‑1 (MQ=60)

                                                                                                                                       CCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTT               <  SRR19973071.3510889/150‑1 (MQ=60)

                                                                                                                                        CTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTG              <  SRR19973071.4150844/150‑1 (MQ=60)

                                                                                                                                        CTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTG              <  SRR19973071.2684472/150‑1 (MQ=60)

                                                                                                                                        CTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTG              <  SRR19973071.4150732/150‑1 (MQ=60)

                                                                                                                                        CTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTG              <  SRR19973071.2016642/150‑1 (MQ=60)

                                                                                                                                           GAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGG           >  SRR19973071.4341826/1‑150 (MQ=60)

                                                                                                                                            AGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGC          >  SRR19973071.1678456/1‑150 (MQ=60)

                                                                                                                                            AGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGC          >  SRR19973071.4078437/1‑150 (MQ=60)

                                                                                                                                             GAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCA         >  SRR19973071.4284451/1‑150 (MQ=60)

                                                                                                                                             GAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCA         >  SRR19973071.1255874/1‑150 (MQ=60)

                                                                                                                                              AATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAA        >  SRR19973071.2554222/1‑150 (MQ=60)

                                                                                                                                               ATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAG       <  SRR19973071.972728/150‑1 (MQ=60)

                                                                                                                                               ATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAG       <  SRR19973071.972350/150‑1 (MQ=60)

                                                                                                                                               ATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAG       <  SRR19973071.3876801/150‑1 (MQ=60)

                                                                                                                                                  AATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGG    <  SRR19973071.3967064/150‑1 (MQ=60)

                                                                                                                                                    TAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCT  <  SRR19973071.841717/150‑1 (MQ=60)

                                                                                                                                                    TAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCT  <  SRR19973071.1274982/150‑1 (MQ=60)

                                                                                                                                                    TAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCT  <  SRR19973071.4424418/150‑1 (MQ=60)

                                                                                                                                                    TAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCT  <  SRR19973071.1275271/150‑1 (MQ=60)

CGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCT  >  CP009273/1768540‑1768837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`