Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,768,689 C→A S215* (TCA→TAA)  ydiB → quinate/shikimate 5‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,768,6890CA100.0% 692.3 / NA 210S215* (TCA→TAA) ydiBquinate/shikimate 5‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (123/87);  total (123/87)

Only 100 of 213 total aligned reads displayed.

CTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGG  >  CP009273/1768543‑1768835
                                                                                                                                                  |                                                                                                                                                  
cTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAAtt                                                                                                                                                 >  2:3322616/1‑150 (MQ=255)
cTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAAtt                                                                                                                                                 >  2:1502288/1‑150 (MQ=255)
 ttCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTg                                                                                                                                                <  2:897848/150‑1 (MQ=255)
   cgcgCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGt                                                                                                                                              <  2:2779698/150‑1 (MQ=255)
    gcgcAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGtt                                                                                                                                             >  2:2400159/1‑150 (MQ=255)
     cgcAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTa                                                                                                                                            >  1:3390379/1‑150 (MQ=255)
     cgaaGCGGGTTAATGAAAACACAGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTa                                                                                                                                            <  2:2432376/147‑1 (MQ=255)
        aGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATg                                                                                                                                         >  1:1318442/1‑150 (MQ=255)
        aGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATg                                                                                                                                         >  1:1707614/1‑150 (MQ=255)
          cGGGTTAATGAAAACACCGAGTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGat                                                                                                                                       <  1:2000775/150‑1 (MQ=255)
            ggTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGatat                                                                                                                                     >  2:2722839/1‑150 (MQ=255)
              ttAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCa                                                                                                                                   <  1:677525/150‑1 (MQ=255)
              ttAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGACGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCa                                                                                                                                   <  1:2582952/150‑1 (MQ=255)
               tAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAg                                                                                                                                  >  1:3644216/1‑150 (MQ=255)
                aaTGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGt                                                                                                                                 <  2:1612766/150‑1 (MQ=255)
                 aTGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTc                                                                                                                                >  1:365145/1‑150 (MQ=255)
                     aaaCACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGtt                                                                                                                            <  1:3029177/150‑1 (MQ=255)
                            gATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCg                                                                                                                     >  2:1866383/1‑150 (MQ=255)
                            gATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCg                                                                                                                     >  2:3633136/1‑150 (MQ=255)
                             aTTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCgg                                                                                                                    <  2:3130520/150‑1 (MQ=255)
                             aTTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCgg                                                                                                                    >  1:851613/1‑150 (MQ=255)
                                  gtcgtcACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACtt                                                                                                               >  1:2754256/1‑150 (MQ=255)
                                     gtcACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTg                                                                                                            >  1:1779953/1‑150 (MQ=255)
                                         cGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCa                                                                                                        <  1:378687/150‑1 (MQ=255)
                                           gTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACt                                                                                                      <  2:2862475/150‑1 (MQ=255)
                                           gTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACt                                                                                                      >  2:3352570/1‑150 (MQ=255)
                                            tCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATCTCAGTCTGTTACATCCGGGACTTCTGGTCACTg                                                                                                     >  1:3316139/1‑150 (MQ=255)
                                            tCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTg                                                                                                     >  2:628110/1‑150 (MQ=255)
                                             cACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGa                                                                                                    <  2:3534095/150‑1 (MQ=255)
                                                  aTCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCg                                                                                               <  1:2983143/150‑1 (MQ=255)
                                                      cGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGta                                                                                           <  2:2165331/150‑1 (MQ=255)
                                                          gATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAAc                                                                                       <  1:2645724/150‑1 (MQ=255)
                                                            tCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAAccc                                                                                     <  2:366502/150‑1 (MQ=255)
                                                             cAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCg                                                                                    <  2:431651/150‑1 (MQ=255)
                                                                  aGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGGATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCGGTTACATCCGGGACGTCTGGTCACTGAATGCGTGTATACCCCGCagcg                                                                               >  2:1789800/1‑147 (MQ=255)
                                                                   gCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATg                                                                              <  2:1140380/150‑1 (MQ=255)
                                                                   gCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATg                                                                              <  2:1139993/150‑1 (MQ=255)
                                                                     cTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGAc                                                                            >  2:1207109/1‑150 (MQ=255)
                                                                      tttGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACg                                                                           <  1:1057294/150‑1 (MQ=255)
                                                                       ttGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGa                                                                          >  1:2530934/1‑150 (MQ=255)
                                                                        tGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGaa                                                                         <  2:798559/150‑1 (MQ=255)
                                                                          cTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGt                                                                       >  2:1918207/1‑150 (MQ=255)
                                                                             aaGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTAt                                                                    <  2:296335/150‑1 (MQ=255)
                                                                             aaGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTAt                                                                    <  2:3487789/150‑1 (MQ=255)
                                                                             aaGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGACACTGAATGCGAGTATAACCCGCATATGACGAAGTTAt                                                                    >  1:2321679/1‑150 (MQ=255)
                                                                              aGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTAtt                                                                   >  1:53112/1‑150 (MQ=255)
                                                                                cccTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTgc                                                                 >  1:1863614/1‑150 (MQ=255)
                                                                                  cTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTgcag                                                               >  2:1573256/1‑150 (MQ=255)
                                                                                   tGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTgcagc                                                              <  1:3416964/150‑1 (MQ=255)
                                                                                    ggCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTgcagca                                                             >  1:1222884/1‑150 (MQ=255)
                                                                                       ttCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGgc                                                          >  2:1267353/1‑150 (MQ=255)
                                                                                       ttCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGgc                                                          <  1:2077568/150‑1 (MQ=255)
                                                                                        tCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGgcg                                                         <  2:3571790/150‑1 (MQ=255)
                                                                                           gccgACCTTTTAACCCATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGcaa                                                      <  2:2205072/150‑1 (MQ=255)
                                                                                            ccgACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGcaac                                                     >  1:2902540/1‑150 (MQ=255)
                                                                                             cgACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGcaaca                                                    <  2:504813/150‑1 (MQ=255)
                                                                                             cgACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGcaaca                                                    <  2:1991852/150‑1 (MQ=255)
                                                                                              gACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGcaacaa                                                   >  2:1638145/1‑150 (MQ=255)
                                                                                               aCATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCGGGCGCAACAAg                                                  <  2:2383064/150‑1 (MQ=255)
                                                                                                 aTTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCt                                                <  2:129704/150‑1 (MQ=255)
                                                                                                 aTTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCt                                                >  1:2971938/1‑150 (MQ=255)
                                                                                                  ttttAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTg                                               >  2:1951330/1‑150 (MQ=255)
                                                                                                    ttAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGt                                             >  2:437200/1‑150 (MQ=255)
                                                                                                     tAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGtt                                            >  1:1971369/1‑150 (MQ=255)
                                                                                                     tAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGtt                                            >  1:216920/1‑150 (MQ=255)
                                                                                                      aaCCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTg                                           >  1:489262/1‑150 (MQ=255)
                                                                                                      aaCCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTg                                           <  2:1779953/150‑1 (MQ=255)
                                                                                                        ccAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCACCAAGCTGGTTGCa                                         >  2:872446/1‑150 (MQ=255)
                                                                                                         cAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCaa                                        >  1:1360550/1‑150 (MQ=255)
                                                                                                            tGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAAc                                     <  2:421991/150‑1 (MQ=255)
                                                                                                             ggCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACg                                    <  2:631198/150‑1 (MQ=255)
                                                                                                               cacaAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGAt                                  <  1:36805/150‑1 (MQ=255)
                                                                                                                 caAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTg                                >  2:689363/1‑150 (MQ=255)
                                                                                                                  aaaaGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGa                               >  2:2571834/1‑150 (MQ=255)
                                                                                                                   aaaGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGAt                              <  2:1318499/150‑1 (MQ=255)
                                                                                                                   aaaGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGAt                              >  1:1584821/1‑150 (MQ=255)
                                                                                                                     aGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATgg                            >  2:2016633/1‑150 (MQ=255)
                                                                                                                     aGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATgg                            >  2:1673413/1‑150 (MQ=255)
                                                                                                                      gTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGa                           >  1:2310882/1‑150 (MQ=255)
                                                                                                                       tGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGAt                          <  1:615368/150‑1 (MQ=255)
                                                                                                                       tGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGAt                          >  1:3013048/1‑150 (MQ=255)
                                                                                                                        gggTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATa                         >  2:1344608/1‑150 (MQ=255)
                                                                                                                          gTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACg                       <  1:111765/150‑1 (MQ=255)
                                                                                                                              gAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAt                   >  1:1086187/1‑150 (MQ=255)
                                                                                                                              gAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAt                   >  2:2402899/1‑150 (MQ=255)
                                                                                                                              gAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAt                   <  1:3642350/150‑1 (MQ=255)
                                                                                                                                 aCCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgtt                >  2:2487975/1‑150 (MQ=255)
                                                                                                                                  ccccTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttg               >  1:2205102/1‑150 (MQ=255)
                                                                                                                                    ccTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttgtt             >  2:1662857/1‑150 (MQ=255)
                                                                                                                                     cTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttgttg            <  1:365406/150‑1 (MQ=255)
                                                                                                                                     cTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttgttg            >  2:1429279/1‑150 (MQ=255)
                                                                                                                                       tGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTtgtg          <  1:3220333/150‑1 (MQ=255)
                                                                                                                                         agaATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGc        >  2:858999/1‑150 (MQ=255)
                                                                                                                                          gaatgaatAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCa       >  1:2239463/1‑150 (MQ=255)
                                                                                                                                            atgaatAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAg     >  2:1002223/1‑150 (MQ=255)
                                                                                                                                            atgaatAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAg     >  2:2906232/1‑150 (MQ=255)
                                                                                                                                             tgaatAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAgg    >  1:3050141/1‑150 (MQ=255)
                                                                                                                                              gaatAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAggg   <  1:1954976/150‑1 (MQ=255)
                                                                                                                                               aataatTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAgggg  <  2:2646785/150‑1 (MQ=255)
                                                                                                                                               aataatTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAgggg  >  2:2530706/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGG  >  CP009273/1768543‑1768835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCTG  >  CP009273/1768541‑1768838
                                                                                                                                                    |                                                                                                                                                     
GCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAA                                                                                                                                                      <  SRR19973061.3596428/150‑1 (MQ=60)
GCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAA                                                                                                                                                      <  SRR19973061.242597/150‑1 (MQ=60)
GCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAA                                                                                                                                                      <  SRR19973061.3596178/150‑1 (MQ=60)
  CTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATT                                                                                                                                                    >  SRR19973061.423138/1‑150 (MQ=60)
   TTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTG                                                                                                                                                   <  SRR19973061.1386325/150‑1 (MQ=60)
       CGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTA                                                                                                                                               >  SRR19973061.3399090/1‑150 (MQ=60)
          AGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATG                                                                                                                                            >  SRR19973061.2060397/1‑150 (MQ=60)
          AGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATG                                                                                                                                            >  SRR19973061.1321754/1‑150 (MQ=60)
          AGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATG                                                                                                                                            >  SRR19973061.1321811/1‑150 (MQ=60)
          AGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATG                                                                                                                                            >  SRR19973061.1711900/1‑150 (MQ=60)
            CGGGTTAATGAAAACACCGAGTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGAT                                                                                                                                          <  SRR19973061.2005785/150‑1 (MQ=60)
              GGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATAT                                                                                                                                        >  SRR19973061.2592240/1‑150 (MQ=60)
              GGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATAT                                                                                                                                        >  SRR19973061.1876522/1‑150 (MQ=60)
                TTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCA                                                                                                                                      <  SRR19973061.679283/150‑1 (MQ=60)
                TTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGACGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCA                                                                                                                                      <  SRR19973061.2589565/150‑1 (MQ=60)
                 TAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAG                                                                                                                                     >  SRR19973061.3653571/1‑150 (MQ=60)
                 TAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAG                                                                                                                                     >  SRR19973061.3653529/1‑150 (MQ=60)
                  AATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGT                                                                                                                                    >  SRR19973061.1260037/1‑150 (MQ=60)
                   ATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTC                                                                                                                                   >  SRR19973061.3386811/1‑150 (MQ=60)
                   ATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTC                                                                                                                                   >  SRR19973061.366133/1‑150 (MQ=60)
                    TGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCT                                                                                                                                  <  SRR19973061.1968980/150‑1 (MQ=60)
                       AAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTT                                                                                                                               <  SRR19973061.3037008/150‑1 (MQ=60)
                       AAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTT                                                                                                                               <  SRR19973061.3036982/150‑1 (MQ=60)
                               ATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGG                                                                                                                       >  SRR19973061.853832/1‑150 (MQ=60)
                                   TGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACT                                                                                                                   <  SRR19973061.2945837/150‑1 (MQ=60)
                                    GTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTT                                                                                                                  >  SRR19973061.1093806/1‑150 (MQ=60)
                                    GTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTT                                                                                                                  >  SRR19973061.2761350/1‑150 (MQ=60)
                                       GTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTG                                                                                                               >  SRR19973061.1785191/1‑150 (MQ=60)
                                       GTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTG                                                                                                               >  SRR19973061.1784411/1‑150 (MQ=60)
                                         CACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGT                                                                                                             >  SRR19973061.2004025/1‑150 (MQ=60)
                                         CACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGT                                                                                                             >  SRR19973061.2321195/1‑150 (MQ=60)
                                           CGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCA                                                                                                           <  SRR19973061.379721/150‑1 (MQ=60)
                                              TCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATCTCAGTCTGTTACATCCGGGACTTCTGGTCACTG                                                                                                        >  SRR19973061.3324689/1‑150 (MQ=60)
                                                   GATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGC                                                                                                   <  SRR19973061.854415/150‑1 (MQ=60)
                                                   GATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGC                                                                                                   >  SRR19973061.118166/1‑150 (MQ=60)
                                                    ATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCG                                                                                                  <  SRR19973061.2990842/150‑1 (MQ=60)
                                                    ATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCG                                                                                                  <  SRR19973061.2990840/150‑1 (MQ=60)
                                                      CTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTG                                                                                                >  SRR19973061.1746174/1‑150 (MQ=60)
                                                            GATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAAC                                                                                          <  SRR19973061.2652520/150‑1 (MQ=60)
                                                            GATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAAC                                                                                          >  SRR19973061.2472210/1‑150 (MQ=60)
                                                             ATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACC                                                                                         >  SRR19973061.3670850/1‑150 (MQ=60)
                                                             ATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACC                                                                                         >  SRR19973061.3297526/1‑150 (MQ=60)
                                                                     GCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATG                                                                                 <  SRR19973061.2111287/150‑1 (MQ=60)
                                                                        TTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACG                                                                              <  SRR19973061.1060008/150‑1 (MQ=60)
                                                                         TTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGA                                                                             >  SRR19973061.2537381/1‑150 (MQ=60)
                                                                               AAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTAT                                                                       <  SRR19973061.2580620/150‑1 (MQ=60)
                                                                               AAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGACACTGAATGCGAGTATAACCCGCATATGACGAAGTTAT                                                                       >  SRR19973061.2327571/1‑150 (MQ=60)
                                                                                AGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATT                                                                      >  SRR19973061.53317/1‑150 (MQ=60)
                                                                                  CCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGC                                                                    >  SRR19973061.1868264/1‑150 (MQ=60)
                                                                                     TGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGC                                                                 <  SRR19973061.3425734/150‑1 (MQ=60)
                                                                                      GGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCA                                                                >  SRR19973061.1225934/1‑150 (MQ=60)
                                                                                         TTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGC                                                             <  SRR19973061.2082783/150‑1 (MQ=60)
                                                                                            CGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCA                                                          >  SRR19973061.2204237/1‑150 (MQ=60)
                                                                                             GCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAA                                                         <  SRR19973061.3700565/150‑1 (MQ=60)
                                                                                              CCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAAC                                                        <  SRR19973061.1084102/150‑1 (MQ=60)
                                                                                              CCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAAC                                                        >  SRR19973061.2909985/1‑150 (MQ=60)
                                                                                              ACGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCTCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAAC                                                        <  SRR19973061.1653025/150‑1 (MQ=60)
                                                                                               CGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACA                                                       >  SRR19973061.2861196/1‑150 (MQ=60)
                                                                                                  CATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGC                                                    <  SRR19973061.2295873/150‑1 (MQ=60)
                                                                                                   ATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCT                                                   >  SRR19973061.2979600/1‑150 (MQ=60)
                                                                                                       TAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTT                                               >  SRR19973061.217558/1‑150 (MQ=60)
                                                                                                       TAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTT                                               >  SRR19973061.1976303/1‑150 (MQ=60)
                                                                                                        AACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTG                                              >  SRR19973061.490566/1‑150 (MQ=60)
                                                                                                           CAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAA                                           >  SRR19973061.1363981/1‑150 (MQ=60)
                                                                                                                 CACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGAT                                     <  SRR19973061.36946/150‑1 (MQ=60)
                                                                                                                 CACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGAT                                     <  SRR19973061.520226/150‑1 (MQ=60)
                                                                                                                     AAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGAT                                 >  SRR19973061.1588756/1‑150 (MQ=60)
                                                                                                                     AAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGAT                                 >  SRR19973061.173489/1‑150 (MQ=60)
                                                                                                                        GTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGA                              >  SRR19973061.2316741/1‑150 (MQ=60)
                                                                                                                        GTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGA                              >  SRR19973061.58835/1‑150 (MQ=60)
                                                                                                                         TGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGAT                             >  SRR19973061.3355149/1‑150 (MQ=60)
                                                                                                                         TGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGAT                             >  SRR19973061.720295/1‑150 (MQ=60)
                                                                                                                         TGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGAT                             >  SRR19973061.3020827/1‑150 (MQ=60)
                                                                                                                         TGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGAT                             <  SRR19973061.616949/150‑1 (MQ=60)
                                                                                                                         TGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGAT                             >  SRR19973061.3020768/1‑150 (MQ=60)
                                                                                                                           GGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGTTATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATAC                           <  SRR19973061.2659878/150‑1 (MQ=60)
                                                                                                                            GTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACG                          <  SRR19973061.112139/150‑1 (MQ=60)
                                                                                                                              ATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGC                        >  SRR19973061.3613207/1‑150 (MQ=60)
                                                                                                                                GAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGTGGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAT                      <  SRR19973061.3651730/150‑1 (MQ=60)
                                                                                                                                GAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAT                      <  SRR19973061.3651661/150‑1 (MQ=60)
                                                                                                                                GAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAT                      >  SRR19973061.1088970/1‑150 (MQ=60)
                                                                                                                                GAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAT                      >  SRR19973061.3167115/1‑150 (MQ=60)
                                                                                                                                 AAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATG                     >  SRR19973061.2956051/1‑150 (MQ=60)
                                                                                                                                 AAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATG                     >  SRR19973061.2227992/1‑150 (MQ=60)
                                                                                                                                 AAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATG                     >  SRR19973061.3420354/1‑150 (MQ=60)
                                                                                                                                    CCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTG                  >  SRR19973061.2210642/1‑150 (MQ=60)
                                                                                                                                    CCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTG                  >  SRR19973061.2211474/1‑150 (MQ=60)
                                                                                                                                       CTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTG               <  SRR19973061.366230/150‑1 (MQ=60)
                                                                                                                                       CTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTG               <  SRR19973061.366396/150‑1 (MQ=60)
                                                                                                                                         TGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTG             <  SRR19973061.3228615/150‑1 (MQ=60)
                                                                                                                                         TGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTG             <  SRR19973061.653619/150‑1 (MQ=60)
                                                                                                                                            GAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCA          >  SRR19973061.417692/1‑150 (MQ=60)
                                                                                                                                            GAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCA          >  SRR19973061.2245089/1‑150 (MQ=60)
                                                                                                                                               TGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGG       >  SRR19973061.3057178/1‑150 (MQ=60)
                                                                                                                                               TGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGG       >  SRR19973061.3058025/1‑150 (MQ=60)
                                                                                                                                                GAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGG      <  SRR19973061.1959855/150‑1 (MQ=60)
                                                                                                                                                  ATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGC    >  SRR19973061.1933755/1‑150 (MQ=60)
                                                                                                                                                   TAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCT   >  SRR19973061.2806364/1‑150 (MQ=60)
                                                                                                                                                    AATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCTG  <  SRR19973061.1506051/150‑1 (MQ=60)
                                                                                                                                                    AATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCTG  >  SRR19973061.2588363/1‑150 (MQ=60)
                                                                                                                                                    AATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCTG  >  SRR19973061.2588408/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCTG  >  CP009273/1768541‑1768838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑