Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,768,689 C→A 100% S215* (TCA→TAA)  ydiB → quinate/shikimate 5‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,768,6890CA96.9% 788.5 / 12.0 260S215* (TCA→TAA) ydiBquinate/shikimate 5‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (5/3);  new base A (129/123);  total (134/126)
Fisher's exact test for biased strand distribution p-value = 7.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 262 total aligned reads displayed.

CTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGG  >  CP009273/1768543‑1768835
                                                                                                                                                  |                                                                                                                                                  
cTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCACTGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAAtt                                                                                                                                                 >  2:1311200/1‑150 (MQ=255)
cTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAAtt                                                                                                                                                 >  2:4858805/1‑150 (MQ=255)
 ttCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCTTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTg                                                                                                                                                >  1:2343244/1‑150 (MQ=255)
   cgcgCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGt                                                                                                                                              >  2:162532/1‑150 (MQ=255)
        aGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATg                                                                                                                                         <  2:5039712/150‑1 (MQ=255)
          cGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGat                                                                                                                                       >  2:1192158/1‑150 (MQ=255)
              ttAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCa                                                                                                                                   <  2:1774732/150‑1 (MQ=255)
                aaTGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGATAATGAATAATTGGTTAATGATATCAGt                                                                                                                                 >  1:228108/1‑150 (MQ=255)
                    acaccacCGAGGGTGTCGGCACGGTCACCGCTCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGt                                                                                                                             <  1:2768291/146‑1 (MQ=255)
                    aaaaCACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGt                                                                                                                             <  1:3693209/150‑1 (MQ=255)
                     aaaCACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGATAATGAATAATTGGTTAATGATATCAGTCTGtt                                                                                                                            <  1:2151012/150‑1 (MQ=255)
                        cacCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACa                                                                                                                         >  2:1078069/1‑150 (MQ=255)
                           cGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCAGTGGTTAATGATATCAGTCTGTTACATcc                                                                                                                      >  2:2115246/1‑150 (MQ=255)
                           cGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATcc                                                                                                                      >  2:2820031/1‑150 (MQ=255)
                             aTTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCgg                                                                                                                    >  2:3626522/1‑150 (MQ=255)
                                    cgtcACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCt                                                                                                             >  1:3004707/1‑150 (MQ=255)
                                    cgtcACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCt                                                                                                             >  2:3767089/1‑150 (MQ=255)
                                         cGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCa                                                                                                        >  2:3481187/1‑150 (MQ=255)
                                         cGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCa                                                                                                        <  2:360908/150‑1 (MQ=255)
                                         cGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCa                                                                                                        <  2:3745462/150‑1 (MQ=255)
                                         cGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATAAGTCTGTTACATCCGGGACTTCTGGTCa                                                                                                        <  1:1027315/150‑1 (MQ=255)
                                          ggTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCAc                                                                                                       <  2:3175183/150‑1 (MQ=255)
                                            tCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTg                                                                                                     >  1:985660/1‑150 (MQ=255)
                                             cACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGa                                                                                                    >  2:878320/1‑150 (MQ=255)
                                              aCCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGaa                                                                                                   >  2:4387018/1‑150 (MQ=255)
                                                cGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGATAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATg                                                                                                 <  1:3447459/150‑1 (MQ=255)
                                                cGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATg                                                                                                 <  1:1014300/150‑1 (MQ=255)
                                                cGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATg                                                                                                 <  2:2281901/150‑1 (MQ=255)
                                                cGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATg                                                                                                 <  1:1896814/150‑1 (MQ=255)
                                                 gATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGc                                                                                                <  2:2881573/150‑1 (MQ=255)
                                                  aTCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCg                                                                                               <  2:2397659/150‑1 (MQ=255)
                                                    ctcGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCgtg                                                                                             <  1:1145840/150‑1 (MQ=255)
                                                     tcGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCgtgt                                                                                            >  2:3503881/1‑150 (MQ=255)
                                                      cGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGACTGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACGTCTGGTCACTGAATGCGTGta                                                                                           >  2:4564997/1‑150 (MQ=255)
                                                       gCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGtat                                                                                          >  1:1399391/1‑150 (MQ=255)
                                                        ccGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGtata                                                                                         <  2:3287075/150‑1 (MQ=255)
                                                        ccGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGtata                                                                                         <  2:2992122/150‑1 (MQ=255)
                                                         cGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATaa                                                                                        >  2:1493924/1‑150 (MQ=255)
                                                          gATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAAc                                                                                       >  1:566994/1‑150 (MQ=255)
                                                            tCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAAccc                                                                                     >  1:1853705/1‑150 (MQ=255)
                                                            tCAGCAAGCCTGTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAAccc                                                                                     <  2:4005527/150‑1 (MQ=255)
                                                             cAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCg                                                                                    >  1:4627263/1‑150 (MQ=255)
                                                              aGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGc                                                                                   >  1:424111/1‑150 (MQ=255)
                                                               gCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCa                                                                                  >  1:1346920/1‑150 (MQ=255)
                                                                 aaGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCata                                                                                >  2:3112767/1‑150 (MQ=255)
                                                                   gCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATg                                                                              <  2:4344710/150‑1 (MQ=255)
                                                                     cTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGAc                                                                            >  1:4418012/1‑150 (MQ=255)
                                                                       ttGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGa                                                                          <  1:5141442/150‑1 (MQ=255)
                                                                        tGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGaa                                                                         <  1:1814181/150‑1 (MQ=255)
                                                                          cTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGt                                                                       >  1:1249351/1‑150 (MQ=255)
                                                                              aGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTAtt                                                                   <  1:3797935/150‑1 (MQ=255)
                                                                              aGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTAtt                                                                   <  1:3933885/150‑1 (MQ=255)
                                                                                 ccTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTgca                                                                >  2:275672/1‑150 (MQ=255)
                                                                                   tGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTgcagc                                                              <  1:1629120/150‑1 (MQ=255)
                                                                                   tGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTgcagc                                                              <  2:650557/150‑1 (MQ=255)
                                                                                   tGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTgcagc                                                              >  1:3536626/1‑150 (MQ=255)
                                                                                     gCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGcagcag                                                            <  1:1634163/150‑1 (MQ=255)
                                                                                     gCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGcagcag                                                            <  1:1743012/150‑1 (MQ=255)
                                                                                       ttCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGgc                                                          <  1:108608/150‑1 (MQ=255)
                                                                                             cgACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGcaaca                                                    <  1:3481187/150‑1 (MQ=255)
                                                                                             cgACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGcaaca                                                    >  2:3345503/1‑150 (MQ=255)
                                                                                                cATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGc                                                 >  2:4798758/1‑150 (MQ=255)
                                                                                                    ttAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGt                                             <  1:1962516/150‑1 (MQ=255)
                                                                                                       aCCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGc                                          >  1:2811143/1‑150 (MQ=255)
                                                                                                       aCCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGc                                          >  1:4365533/1‑150 (MQ=255)
                                                                                                       aCCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGc                                          <  2:1695895/150‑1 (MQ=255)
                                                                                                       aCCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGc                                          >  1:2075721/1‑150 (MQ=255)
                                                                                                       aCCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAAACCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGc                                          >  2:5130236/1‑150 (MQ=255)
                                                                                                        ccAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCa                                         <  2:2437497/150‑1 (MQ=255)
                                                                                                        ccAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCa                                         <  2:4986061/150‑1 (MQ=255)
                                                                                                           aTGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCaaaa                                      <  2:4025649/150‑1 (MQ=255)
                                                                                                           aTGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCaaaa                                      >  1:2233163/1‑150 (MQ=255)
                                                                                                            tGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAAc                                     >  1:40969/1‑150 (MQ=255)
                                                                                                                acaAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGAtt                                 <  1:860688/150‑1 (MQ=255)
                                                                                                                 caAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTg                                <  2:3784078/150‑1 (MQ=255)
                                                                                                                 caAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTg                                >  1:678149/1‑150 (MQ=255)
                                                                                                                  aaaaGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGa                               >  1:3865173/1‑150 (MQ=255)
                                                                                                                   aaaGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGAt                              <  1:2421855/150‑1 (MQ=255)
                                                                                                                   aaaGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGAt                              >  1:5071148/1‑150 (MQ=255)
                                                                                                                     aGTGGGTCTGAAACCCCTTGAGAATGAATAAGTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACACGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATgg                            >  1:3603972/1‑150 (MQ=255)
                                                                                                                      gTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGa                           <  1:909932/150‑1 (MQ=255)
                                                                                                                       tGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGAt                          >  2:1045540/1‑150 (MQ=255)
                                                                                                                         ggTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATAc                        <  2:4477315/150‑1 (MQ=255)
                                                                                                                          gTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACg                       <  1:4044706/150‑1 (MQ=255)
                                                                                                                           tATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACgg                      >  2:3829900/1‑150 (MQ=255)
                                                                                                                                 aCCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgtt                >  1:4978605/1‑150 (MQ=255)
                                                                                                                                  ccccTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGGTGGATACGGCAtgttg               <  1:3234507/150‑1 (MQ=255)
                                                                                                                                  ccccTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttg               >  2:3309017/1‑150 (MQ=255)
                                                                                                                                  ccccTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttg               <  1:4933110/150‑1 (MQ=255)
                                                                                                                                   cccTTGAGAATGAATAATAGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttgt              <  1:293196/150‑1 (MQ=255)
                                                                                                                                     cTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttgttg            >  1:4589290/1‑150 (MQ=255)
                                                                                                                                        gagaATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTgg         >  1:2699073/1‑150 (MQ=255)
                                                                                                                                        gagaATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTgg         <  2:549999/150‑1 (MQ=255)
                                                                                                                                          gaatgaatAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCa       <  1:503586/150‑1 (MQ=255)
                                                                                                                                          gaatgaatAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCa       >  1:4693397/1‑150 (MQ=255)
                                                                                                                                           aatgaatAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCaa      <  2:227711/150‑1 (MQ=255)
                                                                                                                                           aatgaatAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCaa      >  2:1090810/1‑150 (MQ=255)
                                                                                                                                              gaatAATTGGTTAATTATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAgtg   <  2:1864949/150‑3 (MQ=255)
                                                                                                                                               aataatTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAgggg  <  2:4245638/150‑1 (MQ=255)
                                                                                                                                               aataatTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAgggg  <  2:4225123/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGG  >  CP009273/1768543‑1768835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCTG  >  CP009273/1768541‑1768838
                                                                                                                                                    |                                                                                                                                                     
GCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAA                                                                                                                                                      >  SRR19973063.2444146/1‑150 (MQ=60)
GCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAA                                                                                                                                                      >  SRR19973063.5002171/1‑150 (MQ=60)
GCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAA                                                                                                                                                      >  SRR19973063.5002143/1‑150 (MQ=60)
   TTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCTTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTG                                                                                                                                                   >  SRR19973063.2350742/1‑150 (MQ=60)
   TTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTG                                                                                                                                                   >  SRR19973063.4037757/1‑150 (MQ=60)
   TTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTG                                                                                                                                                   >  SRR19973063.4038559/1‑150 (MQ=60)
     CGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGT                                                                                                                                                 >  SRR19973063.2306385/1‑150 (MQ=60)
        GCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAA                                                                                                                                              <  SRR19973063.1515396/150‑1 (MQ=60)
          AGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATG                                                                                                                                            <  SRR19973063.412157/150‑1 (MQ=60)
          AGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATG                                                                                                                                            <  SRR19973063.240413/150‑1 (MQ=60)
          AGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATG                                                                                                                                            <  SRR19973063.2452730/150‑1 (MQ=60)
           GCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGA                                                                                                                                           <  SRR19973063.1286944/150‑1 (MQ=60)
             GGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATA                                                                                                                                         >  SRR19973063.4394148/1‑150 (MQ=60)
             GGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATA                                                                                                                                         >  SRR19973063.498000/1‑150 (MQ=60)
                TTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCA                                                                                                                                      <  SRR19973063.1164964/150‑1 (MQ=60)
                TTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCA                                                                                                                                      <  SRR19973063.4262283/150‑1 (MQ=60)
                  AATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGATAATGAATAATTGGTTAATGATATCAGT                                                                                                                                    >  SRR19973063.228793/1‑150 (MQ=60)
                  AATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGATAATGAATAATTGGTTAATGATATCAGT                                                                                                                                    >  SRR19973063.228395/1‑150 (MQ=60)
                   ATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTC                                                                                                                                   >  SRR19973063.3652916/1‑150 (MQ=60)
                   ATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTC                                                                                                                                   <  SRR19973063.1847208/150‑1 (MQ=60)
                   ATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTC                                                                                                                                   >  SRR19973063.3652904/1‑150 (MQ=60)
                      acaccaccgaggGTGTCGGCACGGTCACCGCTCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGT                                                                                                                                <  SRR19973063.2777213/138‑1 (MQ=60)
                      AAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGT                                                                                                                                <  SRR19973063.3705033/150‑1 (MQ=60)
                       AAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGATAATGAATAATTGGTTAATGATATCAGTCTGTT                                                                                                                               <  SRR19973063.2157749/150‑1 (MQ=60)
                        AACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACACCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTA                                                                                                                              <  SRR19973063.1362050/150‑1 (MQ=60)
                          CACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACA                                                                                                                            >  SRR19973063.353153/1‑150 (MQ=60)
                           ACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACAT                                                                                                                           <  SRR19973063.1018659/150‑1 (MQ=60)
                                  GTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCGGTCTGTTACATCCGGGAC                                                                                                                    <  SRR19973063.1813905/150‑1 (MQ=60)
                                      CGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCT                                                                                                                >  SRR19973063.3014294/1‑150 (MQ=60)
                                      CGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCT                                                                                                                >  SRR19973063.1932231/1‑150 (MQ=60)
                                           CGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATAAGTCTGTTACATCCGGGACTTCTGGTCA                                                                                                           <  SRR19973063.1030413/150‑1 (MQ=60)
                                            GGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCAC                                                                                                          >  SRR19973063.1692525/1‑150 (MQ=60)
                                              TCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTG                                                                                                        >  SRR19973063.988632/1‑150 (MQ=60)
                                              TCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTG                                                                                                        >  SRR19973063.988655/1‑150 (MQ=60)
                                               CACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGA                                                                                                       >  SRR19973063.2292646/1‑150 (MQ=60)
                                                  CGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGATAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATG                                                                                                    <  SRR19973063.3458416/150‑1 (MQ=60)
                                                  CGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGATAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATG                                                                                                    <  SRR19973063.3458422/150‑1 (MQ=60)
                                                  CGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATG                                                                                                    <  SRR19973063.1017357/150‑1 (MQ=60)
                                                  CGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATG                                                                                                    >  SRR19973063.3890399/1‑150 (MQ=60)
                                                  CGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATG                                                                                                    <  SRR19973063.1902748/150‑1 (MQ=60)
                                                   GATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGC                                                                                                   >  SRR19973063.2030524/1‑150 (MQ=60)
                                                    ATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCG                                                                                                  <  SRR19973063.4538396/150‑1 (MQ=60)
                                                    ATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCG                                                                                                  >  SRR19973063.3551499/1‑150 (MQ=60)
                                                    ATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCG                                                                                                  <  SRR19973063.4538430/150‑1 (MQ=60)
                                                      CTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTG                                                                                                <  SRR19973063.1149304/150‑1 (MQ=60)
                                                        CGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTA                                                                                              >  SRR19973063.1706662/1‑150 (MQ=60)
                                                         GCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTAT                                                                                             >  SRR19973063.1403623/1‑150 (MQ=60)
                                                           CGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAA                                                                                           >  SRR19973063.3397333/1‑150 (MQ=60)
                                                            GATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAAC                                                                                          >  SRR19973063.568669/1‑150 (MQ=60)
                                                            GATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAAC                                                                                          >  SRR19973063.568589/1‑150 (MQ=60)
                                                            GATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAAC                                                                                          >  SRR19973063.568504/1‑150 (MQ=60)
                                                             ATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACC                                                                                         <  SRR19973063.2897400/150‑1 (MQ=60)
                                                              TCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCC                                                                                        >  SRR19973063.1859494/1‑150 (MQ=60)
                                                               CAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCG                                                                                       <  SRR19973063.4690965/150‑1 (MQ=60)
                                                               CAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCG                                                                                       >  SRR19973063.4642049/1‑150 (MQ=60)
                                                                agctagcctttgatgaagacctgtgttgctCCGACATTTTAAACAATGGCACAAAAGTGGGTATGAAACCACTTGAGAATGAATAATTGGTTAATGATATCAGTCTTTTAAATCCGGGACTTATGGTCACTGAATGCGTGTATAACCCGC                                                                                      <  SRR19973063.5092500/120‑1 (MQ=60)
                                                                AGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGC                                                                                      >  SRR19973063.425298/1‑150 (MQ=60)
                                                                 GCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCA                                                                                     >  SRR19973063.1350965/1‑150 (MQ=60)
                                                                   AAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATA                                                                                   <  SRR19973063.1790635/150‑1 (MQ=60)
                                                                   AAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATA                                                                                   <  SRR19973063.4198619/150‑1 (MQ=60)
                                                                    AGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATAT                                                                                  <  SRR19973063.1502769/150‑1 (MQ=60)
                                                                    AGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATAT                                                                                  >  SRR19973063.3046705/1‑150 (MQ=60)
                                                                     GCCTTTGCTGAAGCCCTGTCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATG                                                                                 >  SRR19973063.3515132/1‑150 (MQ=60)
                                                                      CCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGA                                                                                <  SRR19973063.705735/150‑1 (MQ=60)
                                                                       CTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGAC                                                                               >  SRR19973063.2813064/1‑150 (MQ=60)
                                                                       CTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGAC                                                                               >  SRR19973063.4432197/1‑150 (MQ=60)
                                                                         TTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGA                                                                             <  SRR19973063.866173/150‑1 (MQ=60)
                                                                         TTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGA                                                                             <  SRR19973063.5157984/150‑1 (MQ=60)
                                                                          TGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAA                                                                            <  SRR19973063.1819813/150‑1 (MQ=60)
                                                                           GCTGAAGCCCTGGCTTCCGCCGACATTTTAACCACTGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAG                                                                           <  SRR19973063.4829868/150‑1 (MQ=60)
                                                                           GCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAG                                                                           <  SRR19973063.2255873/150‑1 (MQ=60)
                                                                            CTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGT                                                                          >  SRR19973063.1253077/1‑150 (MQ=60)
                                                                                AGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATT                                                                      <  SRR19973063.3946454/150‑1 (MQ=60)
                                                                                AGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATT                                                                      <  SRR19973063.3810036/150‑1 (MQ=60)
                                                                                AGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATT                                                                      <  SRR19973063.3810037/150‑1 (MQ=60)
                                                                                     TGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGC                                                                 <  SRR19973063.1634094/150‑1 (MQ=60)
                                                                                     TGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGC                                                                 <  SRR19973063.1634158/150‑1 (MQ=60)
                                                                                     TGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGC                                                                 >  SRR19973063.3547959/1‑150 (MQ=60)
                                                                                     TGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGC                                                                 >  SRR19973063.2110263/1‑150 (MQ=60)
                                                                                       GCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAG                                                               <  SRR19973063.1639157/150‑1 (MQ=60)
                                                                                       GCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAG                                                               <  SRR19973063.1748310/150‑1 (MQ=60)
                                                                                         TTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGC                                                             <  SRR19973063.108974/150‑1 (MQ=60)
                                                                                         TTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGC                                                             <  SRR19973063.2713104/150‑1 (MQ=60)
                                                                                          TCCGCCGACATTTTAACCAATGGCACACAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCG                                                            <  SRR19973063.5055473/150‑1 (MQ=60)
                                                                                          TCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCG                                                            <  SRR19973063.2513276/150‑1 (MQ=60)
                                                                                           CCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGC                                                           <  SRR19973063.5131261/150‑1 (MQ=60)
                                                                                               CGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACA                                                       <  SRR19973063.3492289/150‑1 (MQ=60)
                                                                                               CGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACA                                                       >  SRR19973063.196397/1‑150 (MQ=60)
                                                                                                GACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAA                                                      >  SRR19973063.2759214/1‑150 (MQ=60)
                                                                                                   ATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCT                                                   <  SRR19973063.88645/150‑1 (MQ=60)
                                                                                                     TTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGG                                                 >  SRR19973063.3851283/1‑150 (MQ=60)
                                                                                                      TTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGT                                                <  SRR19973063.1968659/150‑1 (MQ=60)
                                                                                                       TAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGTTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTT                                               <  SRR19973063.4775282/150‑1 (MQ=60)
                                                                                                        AACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTG                                              <  SRR19973063.5046482/150‑1 (MQ=60)
                                                                                                        AACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTG                                              <  SRR19973063.1195741/150‑1 (MQ=60)
                                                                                                         ACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGC                                             >  SRR19973063.2082211/1‑150 (MQ=60)
                                                                                                         ACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGC                                             >  SRR19973063.2820177/1‑150 (MQ=60)
                                                                                                         ACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGC                                             >  SRR19973063.4379499/1‑150 (MQ=60)
                                                                                                             ATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAA                                         >  SRR19973063.2240245/1‑150 (MQ=60)
                                                                                                              TGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAAC                                        >  SRR19973063.41127/1‑150 (MQ=60)
                                                                                                              TGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAAC                                        <  SRR19973063.4676273/150‑1 (MQ=60)
                                                                                                               GGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACG                                       <  SRR19973063.3756057/150‑1 (MQ=60)
                                                                                                                  ACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATT                                    <  SRR19973063.1039098/150‑1 (MQ=60)
                                                                                                                  ACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATT                                    <  SRR19973063.863167/150‑1 (MQ=60)
                                                                                                                   CAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTG                                   >  SRR19973063.680134/1‑150 (MQ=60)
                                                                                                                   CAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTG                                   >  SRR19973063.680141/1‑150 (MQ=60)
                                                                                                                    AAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGA                                  >  SRR19973063.3877467/1‑150 (MQ=60)
                                                                                                                     AAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGAT                                 <  SRR19973063.2429598/150‑1 (MQ=60)
                                                                                                                     AAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGAT                                 >  SRR19973063.5087460/1‑150 (MQ=60)
                                                                                                                       AGTGGGTCTGAAACCCCTTGAGAATGAATAAGTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACACGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGAAGG                               >  SRR19973063.3615538/1‑150 (MQ=60)
                                                                                                                       AGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAACACGATTGATGG                               >  SRR19973063.5040881/1‑150 (MQ=60)
                                                                                                                        GTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGA                              <  SRR19973063.912605/150‑1 (MQ=60)
                                                                                                                         TGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGAT                             >  SRR19973063.2909515/1‑150 (MQ=60)
                                                                                                                         TGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGAT                             >  SRR19973063.3941637/1‑150 (MQ=60)
                                                                                                                          GGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATA                            <  SRR19973063.214610/150‑1 (MQ=60)
                                                                                                                           GGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATAC                           <  SRR19973063.3083698/150‑1 (MQ=60)
                                                                                                                            GTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACG                          <  SRR19973063.4057665/150‑1 (MQ=60)
                                                                                                                                 AAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATG                     >  SRR19973063.2179497/1‑150 (MQ=60)
                                                                                                                                   ACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTT                   >  SRR19973063.4994700/1‑150 (MQ=60)
                                                                                                                                   ACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTT                   <  SRR19973063.188652/150‑1 (MQ=60)
                                                                                                                                    CCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGGTGGATACGGCATGTTG                  <  SRR19973063.3244899/150‑1 (MQ=60)
                                                                                                                                    CCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTG                  <  SRR19973063.4949045/150‑1 (MQ=60)
                                                                                                                                     CCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGT                 >  SRR19973063.4628110/1‑150 (MQ=60)
                                                                                                                                     CCCTTGAGAATGAATAATAGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGT                 <  SRR19973063.294064/150‑1 (MQ=60)
                                                                                                                                       CTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTG               >  SRR19973063.1338728/1‑150 (MQ=60)
                                                                                                                                       CTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTG               >  SRR19973063.4603975/1‑150 (MQ=60)
                                                                                                                                         TGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTG             <  SRR19973063.4077516/150‑1 (MQ=60)
                                                                                                                                         TGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTG             >  SRR19973063.1902510/1‑150 (MQ=60)
                                                                                                                                          GAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGG            >  SRR19973063.2707777/1‑150 (MQ=60)
                                                                                                                                          GAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGG            <  SRR19973063.439978/150‑1 (MQ=60)
                                                                                                                                           AGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGC           <  SRR19973063.1078998/150‑1 (MQ=60)
                                                                                                                                           AGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGC           <  SRR19973063.1089920/150‑1 (MQ=60)
                                                                                                                                            GAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCA          <  SRR19973063.505042/150‑1 (MQ=60)
                                                                                                                                            GAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCA          >  SRR19973063.4708359/1‑150 (MQ=60)
                                                                                                                                            GAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCA          >  SRR19973063.3434596/1‑150 (MQ=60)
                                                                                                                                              ATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAG        <  SRR19973063.2972156/150‑1 (MQ=60)
                                                                                                                                                 AATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGG     >  SRR19973063.666133/1‑150 (MQ=60)
                                                                                                                                                 AATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGG     >  SRR19973063.944394/1‑150 (MQ=60)
                                                                                                                                                   TAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCT   <  SRR19973063.4330139/150‑1 (MQ=60)
                                                                                                                                                    AATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCTG  <  SRR19973063.1315108/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCTG  >  CP009273/1768541‑1768838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑