Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,768,689 C→A 100% S215* (TCA→TAA)  ydiB → quinate/shikimate 5‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,768,6890CA98.5% 1017.7 / 3.3 322S215* (TCA→TAA) ydiBquinate/shikimate 5‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/5);  new base A (124/190);  total (124/198)
Fisher's exact test for biased strand distribution p-value = 1.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 329 total aligned reads displayed.

CCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGG  >  CP009273/1768542‑1768835
                                                                                                                                                   |                                                                                                                                                  
 cTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAAtt                                                                                                                                                 >  1:457538/1‑150 (MQ=255)
    cgcgCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGt                                                                                                                                              <  2:4016416/150‑1 (MQ=255)
    cgcgCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGt                                                                                                                                              <  2:4168424/150‑1 (MQ=255)
       gcAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTaa                                                                                                                                           <  1:3477266/150‑1 (MQ=255)
       gcAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTaa                                                                                                                                           <  2:4584342/150‑1 (MQ=255)
        cAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAAt                                                                                                                                          <  2:2612779/150‑1 (MQ=255)
         aGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATg                                                                                                                                         >  2:628901/1‑150 (MQ=255)
             ggTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGatat                                                                                                                                     <  1:2218699/150‑1 (MQ=255)
              gTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATc                                                                                                                                    <  1:3665938/150‑1 (MQ=255)
              gTTAAAGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATc                                                                                                                                    <  2:3530316/150‑1 (MQ=255)
                tAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAg                                                                                                                                  >  2:3849126/1‑150 (MQ=255)
                tAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAg                                                                                                                                  <  2:1853819/150‑1 (MQ=255)
                   tGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCt                                                                                                                               <  2:3159936/150‑1 (MQ=255)
                      aaaCACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGtt                                                                                                                            <  1:5204531/150‑1 (MQ=255)
                            cGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGTCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATcc                                                                                                                      >  1:1573187/1‑150 (MQ=255)
                            cGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATcc                                                                                                                      <  2:5043908/150‑1 (MQ=255)
                            cGAGTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATcc                                                                                                                      <  1:1847463/150‑1 (MQ=255)
                               ttGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCggg                                                                                                                   <  1:2952927/150‑1 (MQ=255)
                                 gtgtCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGAc                                                                                                                 <  2:2380172/150‑1 (MQ=255)
                                   gtcgtcACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACtt                                                                                                               <  1:3513324/150‑1 (MQ=255)
                                     cgtcACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCt                                                                                                             <  1:379388/150‑1 (MQ=255)
                                     cgtcACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCt                                                                                                             <  2:2148518/150‑1 (MQ=255)
                                      gtcACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTg                                                                                                            <  1:3627197/150‑1 (MQ=255)
                                      gtcACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTg                                                                                                            >  1:1249496/1‑150 (MQ=255)
                                        cACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGt                                                                                                          <  1:4018986/150‑1 (MQ=255)
                                            gTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACt                                                                                                      <  2:3407041/150‑1 (MQ=255)
                                            gTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACt                                                                                                      <  2:1443076/150‑1 (MQ=255)
                                             tCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTGCATCCGGGACTTCTGGTCACTg                                                                                                     <  2:7772/150‑1 (MQ=255)
                                               aCCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGaa                                                                                                   >  2:3025993/1‑150 (MQ=255)
                                               aCCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACACCTAGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGaa                                                                                                   >  1:151383/1‑150 (MQ=255)
                                                 cGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATg                                                                                                 <  2:1085229/150‑1 (MQ=255)
                                                  gATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGc                                                                                                >  2:1272105/1‑150 (MQ=255)
                                                  gATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGc                                                                                                >  2:4551232/1‑150 (MQ=255)
                                                  gATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTGGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGc                                                                                                >  2:309480/1‑150 (MQ=255)
                                                        gCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGtat                                                                                          <  1:3597824/150‑1 (MQ=255)
                                                         ccGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGtata                                                                                         <  2:1798921/150‑1 (MQ=255)
                                                           gATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAAc                                                                                       >  1:1806448/1‑150 (MQ=255)
                                                             tCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAAccc                                                                                     <  2:1636767/150‑1 (MQ=255)
                                                                 cAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTCACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCat                                                                                 <  2:4460617/150‑1 (MQ=255)
                                                                    gCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATg                                                                              <  2:2707292/150‑1 (MQ=255)
                                                                     ccTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGa                                                                             <  2:313645/150‑1 (MQ=255)
                                                                      cTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGAc                                                                            <  1:2305189/150‑1 (MQ=255)
                                                                      cTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGAc                                                                            <  2:1366276/150‑1 (MQ=255)
                                                                         tGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGaa                                                                         <  1:4133707/150‑1 (MQ=255)
                                                                          gCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAg                                                                        <  1:2787840/150‑1 (MQ=255)
                                                                            tGAAGCCCTGGCTTCCGCCGACATTTTAACCAATTGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATACTTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGtt                                                                      <  2:1824970/150‑1 (MQ=255)
                                                                            tGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTTGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGAGTGTATAACCCGCATATGACGAAGtt                                                                      >  1:1892944/1‑150 (MQ=255)
                                                                             gAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTa                                                                     >  1:2148710/1‑150 (MQ=255)
                                                                             gAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTa                                                                     <  2:4249178/150‑1 (MQ=255)
                                                                               aGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTAtt                                                                   >  2:1194345/1‑150 (MQ=255)
                                                                                 cccTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTgc                                                                 <  2:3619627/150‑1 (MQ=255)
                                                                                  ccTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTgca                                                                <  1:957568/150‑1 (MQ=255)
                                                                                    tGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTgcagc                                                              >  1:5278694/1‑150 (MQ=255)
                                                                                    tGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTgcagc                                                              >  1:3915788/1‑150 (MQ=255)
                                                                                    tGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTgcagc                                                              <  1:2441836/150‑1 (MQ=255)
                                                                                      gCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGcagcag                                                            <  2:3888030/150‑1 (MQ=255)
                                                                                             ccgACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGcaac                                                     <  2:1035367/150‑1 (MQ=255)
                                                                                                aCATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAg                                                  <  1:1115826/150‑1 (MQ=255)
                                                                                                aCATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAg                                                  <  1:201932/150‑1 (MQ=255)
                                                                                                  aTTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCt                                                >  2:1008189/1‑150 (MQ=255)
                                                                                                  aTTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCt                                                >  1:2634814/1‑150 (MQ=255)
                                                                                                  aTTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCt                                                <  1:1182479/150‑1 (MQ=255)
                                                                                                   ttttAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTg                                               <  1:1410959/150‑1 (MQ=255)
                                                                                                    tttAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTgg                                              >  2:2768352/1‑150 (MQ=255)
                                                                                                       aaCCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTg                                           >  1:2324856/1‑150 (MQ=255)
                                                                                                         ccAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCa                                         >  1:2540755/1‑150 (MQ=255)
                                                                                                         ccAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCa                                         <  1:3488604/150‑1 (MQ=255)
                                                                                                           aaTGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCaaa                                       >  1:3063560/1‑150 (MQ=255)
                                                                                                             tGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAAc                                     <  1:3807708/150‑1 (MQ=255)
                                                                                                              ggCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACg                                    <  1:4331956/150‑1 (MQ=255)
                                                                                                                cacaAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGAt                                  >  2:5202309/1‑150 (MQ=255)
                                                                                                                 acaAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGAtt                                 <  2:4861632/150‑1 (MQ=255)
                                                                                                                  caAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTg                                <  2:3296308/150‑1 (MQ=255)
                                                                                                                  caAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTg                                >  1:3377255/1‑150 (MQ=255)
                                                                                                                   aaaaGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGa                               <  1:75467/150‑1 (MQ=255)
                                                                                                                   aaaaGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGa                               >  2:5317732/1‑150 (MQ=255)
                                                                                                                    aaaGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGAt                              <  2:22293/150‑1 (MQ=255)
                                                                                                                     aaGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATg                             <  2:4298451/150‑1 (MQ=255)
                                                                                                                        tGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGAt                          <  1:3697405/150‑1 (MQ=255)
                                                                                                                             aTGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGc                     <  1:4909884/150‑1 (MQ=255)
                                                                                                                               gAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAt                   >  2:1383952/1‑150 (MQ=255)
                                                                                                                                aaaCCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtg                  <  1:169510/150‑1 (MQ=255)
                                                                                                                                aaaCCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtg                  >  2:659794/1‑150 (MQ=255)
                                                                                                                                  aCCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgtt                <  2:3777679/150‑1 (MQ=255)
                                                                                                                                   ccccTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttg               <  2:2419368/150‑1 (MQ=255)
                                                                                                                                   ccccTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttg               <  1:1058307/150‑1 (MQ=255)
                                                                                                                                   ccccTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttg               <  2:1936287/150‑1 (MQ=255)
                                                                                                                                    cccTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttgt              <  2:264689/150‑1 (MQ=255)
                                                                                                                                     ccTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttgtt             >  2:3675133/1‑150 (MQ=255)
                                                                                                                                      cTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttgttg            <  2:4742070/150‑1 (MQ=255)
                                                                                                                                      cTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttgttg            >  1:3197708/1‑150 (MQ=255)
                                                                                                                                       ttGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCAtgttgttgt           >  1:4602210/1‑150 (MQ=255)
                                                                                                                                        tGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTtgtg          >  1:4726804/1‑150 (MQ=255)
                                                                                                                                        tGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTtgtg          <  2:2884695/150‑1 (MQ=255)
                                                                                                                                          agaATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGc        >  2:841703/1‑150 (MQ=255)
                                                                                                                                              tgaatAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAgg    >  2:4746343/1‑150 (MQ=255)
                                                                                                                                                aataatTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAgggg  <  1:2715930/150‑1 (MQ=255)
                                                                                                                                                aataatTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAgggg  >  1:906679/1‑150 (MQ=255)
                                                                                                                                                aataatTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAgggg  >  2:2205365/1‑150 (MQ=255)
                                                                                                                                                aataatTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAgggg  <  2:2120385/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGG  >  CP009273/1768542‑1768835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCTG  >  CP009273/1768542‑1768838
                                                                                                                                                   |                                                                                                                                                     
CCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAAT                                                                                                                                                     <  SRR19973062.1067928/150‑1 (MQ=60)
 CTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATT                                                                                                                                                    <  SRR19973062.1197835/150‑1 (MQ=60)
 CTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATT                                                                                                                                                    >  SRR19973062.459535/1‑150 (MQ=60)
  TTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCTAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTG                                                                                                                                                   <  SRR19973062.2331870/150‑1 (MQ=60)
  TTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTG                                                                                                                                                   >  SRR19973062.308392/1‑150 (MQ=60)
      CGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTA                                                                                                                                               <  SRR19973062.3318379/150‑1 (MQ=60)
       GCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAA                                                                                                                                              <  SRR19973062.3494955/150‑1 (MQ=60)
       GCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAA                                                                                                                                              <  SRR19973062.3495107/150‑1 (MQ=60)
       GCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAA                                                                                                                                              >  SRR19973062.3703401/1‑150 (MQ=60)
        CAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAAT                                                                                                                                             <  SRR19973062.5035320/150‑1 (MQ=60)
        CAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAAT                                                                                                                                             <  SRR19973062.3779406/150‑1 (MQ=60)
        CAGCGGGATAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAAT                                                                                                                                             <  SRR19973062.3779405/150‑1 (MQ=60)
         AGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCTCAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATG                                                                                                                                            <  SRR19973062.3486384/150‑1 (MQ=60)
           CGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGAT                                                                                                                                          >  SRR19973062.2899925/1‑150 (MQ=60)
           CGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGAT                                                                                                                                          <  SRR19973062.1939001/150‑1 (MQ=60)
           CGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGAT                                                                                                                                          <  SRR19973062.1938791/150‑1 (MQ=60)
            GGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATA                                                                                                                                         >  SRR19973062.3629474/1‑150 (MQ=60)
             GGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATAT                                                                                                                                        <  SRR19973062.2229815/150‑1 (MQ=60)
              GTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATC                                                                                                                                       <  SRR19973062.3684737/150‑1 (MQ=60)
              GTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATC                                                                                                                                       <  SRR19973062.959300/150‑1 (MQ=60)
                TAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAG                                                                                                                                     <  SRR19973062.3931891/150‑1 (MQ=60)
                 AATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGT                                                                                                                                    <  SRR19973062.3000028/150‑1 (MQ=60)
                  ATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTC                                                                                                                                   >  SRR19973062.2304178/1‑150 (MQ=60)
                    GAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCCGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTG                                                                                                                                 <  SRR19973062.1633495/150‑1 (MQ=60)
                      AAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTT                                                                                                                               <  SRR19973062.5231995/150‑1 (MQ=60)
                       AACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTCACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTA                                                                                                                              <  SRR19973062.5163974/150‑1 (MQ=60)
                        ACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAGCCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTAC                                                                                                                             <  SRR19973062.3501894/150‑1 (MQ=60)
                         CACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACA                                                                                                                            >  SRR19973062.5216060/1‑150 (MQ=60)
                            CGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGTCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCC                                                                                                                         >  SRR19973062.1580967/1‑150 (MQ=60)
                            CGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGTCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCC                                                                                                                         >  SRR19973062.1580957/1‑150 (MQ=60)
                            CGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCC                                                                                                                         <  SRR19973062.1586170/150‑1 (MQ=60)
                            CGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCC                                                                                                                         <  SRR19973062.2182508/150‑1 (MQ=60)
                            CGAGTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCC                                                                                                                         <  SRR19973062.1856686/150‑1 (MQ=60)
                             GATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCG                                                                                                                        >  SRR19973062.2697164/1‑150 (MQ=60)
                               TTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGG                                                                                                                      <  SRR19973062.2968439/150‑1 (MQ=60)
                                   GTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTT                                                                                                                  <  SRR19973062.3531129/150‑1 (MQ=60)
                                     CGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCT                                                                                                                >  SRR19973062.1295173/1‑150 (MQ=60)
                                     CGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCT                                                                                                                <  SRR19973062.4579263/150‑1 (MQ=60)
                                     CGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCT                                                                                                                <  SRR19973062.380673/150‑1 (MQ=60)
                                     CGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCT                                                                                                                <  SRR19973062.381081/150‑1 (MQ=60)
                                      GTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTG                                                                                                               >  SRR19973062.1255623/1‑150 (MQ=60)
                                      GTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTG                                                                                                               <  SRR19973062.3645741/150‑1 (MQ=60)
                                      GTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTG                                                                                                               >  SRR19973062.2247049/1‑150 (MQ=60)
                                      GTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTG                                                                                                               >  SRR19973062.1255272/1‑150 (MQ=60)
                                        CACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGT                                                                                                             <  SRR19973062.4039318/150‑1 (MQ=60)
                                           GGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCAC                                                                                                          <  SRR19973062.3643571/150‑1 (MQ=60)
                                             TCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTG                                                                                                        <  SRR19973062.3302627/150‑1 (MQ=60)
                                               ACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAA                                                                                                      >  SRR19973062.3520395/1‑150 (MQ=60)
                                               ACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACACCTAGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAA                                                                                                      >  SRR19973062.152257/1‑150 (MQ=60)
                                                   ATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCG                                                                                                  <  SRR19973062.1736404/150‑1 (MQ=60)
                                                   ATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCG                                                                                                  >  SRR19973062.3940831/1‑150 (MQ=60)
                                                        GCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTAT                                                                                             <  SRR19973062.3616104/150‑1 (MQ=60)
                                                         CCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATA                                                                                            >  SRR19973062.1820291/1‑150 (MQ=60)
                                                           GATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAAC                                                                                          >  SRR19973062.1815220/1‑150 (MQ=60)
                                                             TCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCC                                                                                        <  SRR19973062.1756313/150‑1 (MQ=60)
                                                              CAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCG                                                                                       <  SRR19973062.2640890/150‑1 (MQ=60)
                                                                  AAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATA                                                                                   >  SRR19973062.2021104/1‑150 (MQ=60)
                                                                   AGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATAT                                                                                  <  SRR19973062.4490272/150‑1 (MQ=60)
                                                                    GCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATG                                                                                 <  SRR19973062.3146350/150‑1 (MQ=60)
                                                                      CTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGAC                                                                               <  SRR19973062.2317252/150‑1 (MQ=60)
                                                                      CTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGAC                                                                               >  SRR19973062.4152999/1‑150 (MQ=60)
                                                                      CTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGAC                                                                               >  SRR19973062.1500589/1‑150 (MQ=60)
                                                                      CTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGAC                                                                               >  SRR19973062.2949192/1‑150 (MQ=60)
                                                                         TGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAA                                                                            <  SRR19973062.4155117/150‑1 (MQ=60)
                                                                          GCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAG                                                                           <  SRR19973062.998348/150‑1 (MQ=60)
                                                                          GCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAG                                                                           <  SRR19973062.2802566/150‑1 (MQ=60)
                                                                          GCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAG                                                                           <  SRR19973062.2802418/150‑1 (MQ=60)
                                                                           CTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGT                                                                          >  SRR19973062.2051486/1‑150 (MQ=60)
                                                                           CTGAAGCCCTGGCTTCCGCCGACAATTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGT                                                                          <  SRR19973062.4789542/150‑1 (MQ=60)
                                                                            TGAAGCCCTGGCTTCCGCCGACATTTTCACCAATGGCACAATAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGTAGTT                                                                         <  SRR19973062.2555161/150‑1 (MQ=60)
                                                                            TGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTTGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGAGTGTATAACCCGCATATGACGAAGTT                                                                         >  SRR19973062.1902578/1‑150 (MQ=60)
                                                                            TGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTT                                                                         >  SRR19973062.1902466/1‑150 (MQ=60)
                                                                             GAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTA                                                                        >  SRR19973062.2159604/1‑150 (MQ=60)
                                                                               AGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATT                                                                      <  SRR19973062.1271834/150‑1 (MQ=60)
                                                                                GCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTG                                                                     <  SRR19973062.3485015/150‑1 (MQ=60)
                                                                                GCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTG                                                                     >  SRR19973062.205938/1‑150 (MQ=60)
                                                                                 CCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGC                                                                    >  SRR19973062.592087/1‑150 (MQ=60)
                                                                                  CCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCA                                                                   <  SRR19973062.3543563/150‑1 (MQ=60)
                                                                                  CCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCA                                                                   <  SRR19973062.961961/150‑1 (MQ=60)
                                                                                    TGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGC                                                                 >  SRR19973062.5306457/1‑150 (MQ=60)
                                                                                    TGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGC                                                                 <  SRR19973062.2454941/150‑1 (MQ=60)
                                                                                    TGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGC                                                                 <  SRR19973062.4428660/150‑1 (MQ=60)
                                                                                    TGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGC                                                                 >  SRR19973062.887500/1‑150 (MQ=60)
                                                                                    TGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGC                                                                 <  SRR19973062.1548512/150‑1 (MQ=60)
                                                                                    TGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGC                                                                 >  SRR19973062.4014257/1‑150 (MQ=60)
                                                                                    TGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGC                                                                 >  SRR19973062.3936105/1‑150 (MQ=60)
                                                                                    TGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGC                                                                 >  SRR19973062.3935722/1‑150 (MQ=60)
                                                                                        TTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGC                                                             <  SRR19973062.1111635/150‑1 (MQ=60)
                                                                                        TTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGC                                                             <  SRR19973062.1854113/150‑1 (MQ=60)
                                                                                         TCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCG                                                            <  SRR19973062.2332145/150‑1 (MQ=60)
                                                                                             CCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCTGCAGGCGCAAC                                                        <  SRR19973062.5317384/150‑1 (MQ=60)
                                                                                              CGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACA                                                       <  SRR19973062.4770973/150‑1 (MQ=60)
                                                                                               GACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAA                                                      >  SRR19973062.368519/1‑150 (MQ=60)
                                                                                                ACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAG                                                     <  SRR19973062.1121063/150‑1 (MQ=60)
                                                                                                ACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAG                                                     <  SRR19973062.203017/150‑1 (MQ=60)
                                                                                                  ATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCT                                                   >  SRR19973062.897750/1‑150 (MQ=60)
                                                                                                  ATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCT                                                   >  SRR19973062.2648691/1‑150 (MQ=60)
                                                                                                  ATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCT                                                   <  SRR19973062.1188010/150‑1 (MQ=60)
                                                                                                  ATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCT                                                   <  SRR19973062.1252225/150‑1 (MQ=60)
                                                                                                   TTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTG                                                  <  SRR19973062.1417729/150‑1 (MQ=60)
                                                                                                      TAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTT                                               >  SRR19973062.4247926/1‑150 (MQ=60)
                                                                                                       AACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTG                                              >  SRR19973062.2337149/1‑150 (MQ=60)
                                                                                                       AACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTG                                              <  SRR19973062.1481684/150‑1 (MQ=60)
                                                                                                       AACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTG                                              >  SRR19973062.2644471/1‑150 (MQ=60)
                                                                                                       AACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTG                                              >  SRR19973062.4718929/1‑150 (MQ=60)
                                                                                                         CCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCA                                            <  SRR19973062.3506535/150‑1 (MQ=60)
                                                                                                         CCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCA                                            <  SRR19973062.3506326/150‑1 (MQ=60)
                                                                                                         CCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCA                                            >  SRR19973062.2554300/1‑150 (MQ=60)
                                                                                                         CCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCA                                            >  SRR19973062.2554185/1‑150 (MQ=60)
                                                                                                           AATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAA                                          >  SRR19973062.3079447/1‑150 (MQ=60)
                                                                                                           AATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAA                                          >  SRR19973062.770658/1‑150 (MQ=60)
                                                                                                            ATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCTTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGATGGTTGCAAAA                                         >  SRR19973062.1872457/1‑150 (MQ=60)
                                                                                                             TGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAAC                                        <  SRR19973062.1141947/150‑1 (MQ=60)
                                                                                                             TGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAAC                                        <  SRR19973062.3827188/150‑1 (MQ=60)
                                                                                                             TGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAAC                                        <  SRR19973062.3827119/150‑1 (MQ=60)
                                                                                                             TGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAAC                                        >  SRR19973062.240593/1‑150 (MQ=60)
                                                                                                              GGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACG                                       <  SRR19973062.4354263/150‑1 (MQ=60)
                                                                                                                CACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGAT                                     <  SRR19973062.2855530/150‑1 (MQ=60)
                                                                                                                 ACAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGAGT                                    >  SRR19973062.4620724/1‑150 (MQ=60)
                                                                                                                  CAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTG                                   <  SRR19973062.2988591/150‑1 (MQ=60)
                                                                                                                  CAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTG                                   >  SRR19973062.3394626/1‑150 (MQ=60)
                                                                                                                  CAAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTG                                   >  SRR19973062.355000/1‑150 (MQ=60)
                                                                                                                   AAAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGA                                  <  SRR19973062.75967/150‑1 (MQ=60)
                                                                                                                    AAAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGAT                                 >  SRR19973062.4675183/1‑150 (MQ=60)
                                                                                                                     AAGTGGGTATGAAACCCCTTGAGAATGAATTATTGGTTAATGATATCAGTCTGTTACATCCGGGTCTTCTGGTCTCTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCATCAAGCTGGTTGCAAAACGATTGATG                                <  SRR19973062.3675587/150‑1 (MQ=60)
                                                                                                                     AAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTGCATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATG                                <  SRR19973062.5242613/150‑1 (MQ=60)
                                                                                                                     AAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTGCATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATG                                <  SRR19973062.5242502/150‑1 (MQ=60)
                                                                                                                     AAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATG                                <  SRR19973062.987371/150‑1 (MQ=60)
                                                                                                                     AAGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATG                                <  SRR19973062.3675483/150‑1 (MQ=60)
                                                                                                                      AGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGG                               <  SRR19973062.1183123/150‑1 (MQ=60)
                                                                                                                      AGTGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGG                               <  SRR19973062.2549466/150‑1 (MQ=60)
                                                                                                                        TGGGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGAT                             <  SRR19973062.3716383/150‑1 (MQ=60)
                                                                                                                          GGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGTAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATAC                           <  SRR19973062.226481/150‑1 (MQ=60)
                                                                                                                          GGTATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATAC                           >  SRR19973062.2731734/1‑150 (MQ=60)
                                                                                                                            TATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGG                         >  SRR19973062.4604296/1‑150 (MQ=60)
                                                                                                                            TATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGACACTGAATGCGTGTATAACCCGCATATGACGAAGTAATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGG                         >  SRR19973062.2160181/1‑150 (MQ=60)
                                                                                                                             ATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGC                        >  SRR19973062.3462931/1‑150 (MQ=60)
                                                                                                                             ATGAAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGC                        <  SRR19973062.4935284/150‑1 (MQ=60)
                                                                                                                                AAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATG                     <  SRR19973062.170451/150‑1 (MQ=60)
                                                                                                                                AAACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATG                     >  SRR19973062.2706574/1‑150 (MQ=60)
                                                                                                                                 AACCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGT                    >  SRR19973062.1679507/1‑150 (MQ=60)
                                                                                                                                   CCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTG                  <  SRR19973062.1063282/150‑1 (MQ=60)
                                                                                                                                   CCCCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTG                  <  SRR19973062.3552439/150‑1 (MQ=60)
                                                                                                                                     CCTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTT                <  SRR19973062.3815381/150‑1 (MQ=60)
                                                                                                                                      CTTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTG               >  SRR19973062.3214307/1‑150 (MQ=60)
                                                                                                                                       TTGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGT              >  SRR19973062.4626289/1‑150 (MQ=60)
                                                                                                                                        TGAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTG             >  SRR19973062.4751468/1‑150 (MQ=60)
                                                                                                                                         GAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGG            >  SRR19973062.404128/1‑150 (MQ=60)
                                                                                                                                         GAGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGG            <  SRR19973062.3868799/150‑1 (MQ=60)
                                                                                                                                          AGAATGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGC           <  SRR19973062.2389708/150‑1 (MQ=60)
                                                                                                                                              TGAATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGG       >  SRR19973062.4324945/1‑150 (MQ=60)
                                                                                                                                                AATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGG     >  SRR19973062.910611/1‑150 (MQ=60)
                                                                                                                                                AATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGG     >  SRR19973062.910640/1‑150 (MQ=60)
                                                                                                                                                AATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGG     <  SRR19973062.2730222/150‑1 (MQ=60)
                                                                                                                                                 ATAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGC    >  SRR19973062.4343167/1‑150 (MQ=60)
                                                                                                                                                  TAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCT   <  SRR19973062.1831914/150‑1 (MQ=60)
                                                                                                                                                  TAATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCT   <  SRR19973062.3674043/150‑1 (MQ=60)
                                                                                                                                                   AATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCTG  <  SRR19973062.335910/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
CCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGCACAAAAGTGGGTATGAAACCCCTTGAGAATGAATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCTG  >  CP009273/1768542‑1768838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑