Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,707 C→A 16.1% intergenic (‑24/+284) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7070CA16.1% 416.3 / 7.6 199intergenic (‑24/+284)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (31/107);  new base A (27/4);  total (88/111)
Fisher's exact test for biased strand distribution p-value = 2.18e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 203 total aligned reads displayed.

ATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCG  >  CP009273/2170558‑2170855
                                                                                                                                                      |                                                                                                                                                     
aTGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTc                                                                                                                                                       <  1:3931481/150‑1 (MQ=255)
 tGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCg                                                                                                                                                      <  1:68567/150‑1 (MQ=255)
 tGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCg                                                                                                                                                      <  1:2495455/150‑1 (MQ=255)
  gCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGa                                                                                                                                                     <  1:587036/150‑1 (MQ=255)
    aGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGAtt                                                                                                                                                   <  2:2034091/150‑1 (MQ=255)
    aGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGAtt                                                                                                                                                   <  1:1425126/150‑1 (MQ=255)
        tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCaa                                                                                                                                               <  1:3121849/150‑1 (MQ=255)
         ggCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGATTTaaaa                                                                                                                                              >  1:4300432/1‑150 (MQ=255)
          gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCaaaa                                                                                                                                             <  1:3815552/150‑1 (MQ=255)
          gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCaaaa                                                                                                                                             <  2:2160898/150‑1 (MQ=255)
           cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAat                                                                                                                                            <  1:2077791/150‑1 (MQ=255)
           cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAat                                                                                                                                            <  1:3429503/150‑1 (MQ=255)
            aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAata                                                                                                                                           <  2:70003/150‑1 (MQ=255)
             gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAatat                                                                                                                                          <  1:3779200/150‑1 (MQ=255)
             gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAatat                                                                                                                                          <  2:1418018/150‑1 (MQ=255)
              cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAAtata                                                                                                                                         <  2:3208805/150‑1 (MQ=255)
               ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATaa                                                                                                                                        <  1:528340/150‑1 (MQ=255)
                gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAAt                                                                                                                                       <  2:4275174/150‑1 (MQ=255)
                 tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATg                                                                                                                                      <  2:494736/150‑1 (MQ=255)
                 tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGT‑GTGGTTTTGATTTCAAAATATAATg                                                                                                                                      >  1:892431/1‑150 (MQ=255)
                   tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                    <  1:4232332/150‑1 (MQ=255)
                    cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGaaa                                                                                                                                   <  1:392124/150‑1 (MQ=255)
                      accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGATTTCAAAATATAATTAAatt                                                                                                                                 >  2:1697004/1‑150 (MQ=255)
                         accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                              <  1:3021804/150‑1 (MQ=255)
                           cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTg                                                                                                                            <  2:1909909/150‑1 (MQ=255)
                           cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTg                                                                                                                            <  1:1463304/150‑1 (MQ=255)
                           cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTg                                                                                                                            <  2:239962/150‑1 (MQ=255)
                             cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGAATTTCAAAAATTATTAAAAtttttttt                                                                                                                           >  1:3720255/1‑148 (MQ=255)
                              ttGCATCGTTTAGAGATTGTTAATATTGAATGCCTGAACCTCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                         <  2:1162512/150‑1 (MQ=255)
                              ttGAATCGTTTCGAGATTGTGAATATTGAATTCCGTAACCGCATAACCGCCGAGCTGTTCGTTGTTCAGCATCTGCTTTTTCGATACCACGTTCATTTTCATATCCTGTCGT‑TTGTTTTAGATTTCAAAATATAATTAAATTATTTGttt                                                                                                                         <  1:2263480/150‑1 (MQ=255)
                               tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTATATTTAAAAAAATAATAAAAAAAtttttttt                                                                                                                        >  1:3319944/1‑150 (MQ=255)
                                gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTGGATTTCAAAAAATAAATAAATTATtttttttt                                                                                                                       >  2:1119086/1‑150 (MQ=255)
                                     gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGATTTAAAAATATAATGAAATTATTTGTTTTTAAata                                                                                                                  >  2:1772703/1‑150 (MQ=255)
                                     gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTCTATTTCAAAATATAATGAAATTTTTTTTTTTTTAata                                                                                                                  >  1:1724997/1‑150 (MQ=255)
                                      tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                 <  2:1706476/150‑1 (MQ=255)
                                       ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                <  1:2308078/150‑1 (MQ=255)
                                       gtCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                <  1:1556197/149‑1 (MQ=255)
                                         cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTCGATTTAAAAATATAATTTAATTAATTTTTTTTAAatataca                                                                                                              >  2:3075640/1‑147 (MQ=255)
                                          gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGCTTTCAAAATATAATTAAATTATTTTTTTTTAAATTTagag                                                                                                             >  2:2705708/1‑150 (MQ=255)
                                           agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgag‑a                                                                                                           <  2:3317504/150‑1 (MQ=255)
                                           agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGATTTAAAAAAATAATGAAATTATTTTTTTTTAAATATAgag‑a                                                                                                           >  2:4198817/1‑150 (MQ=255)
                                              ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTAGAATTGAAAAAAAAAATAAATTATTTTTTTTTAAATATAGAG‑aaaa                                                                                                        >  2:3399254/1‑150 (MQ=255)
                                               tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAAc                                                                                                       <  2:2486686/150‑1 (MQ=255)
                                               tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAAc                                                                                                       <  2:3720598/150‑1 (MQ=255)
                                                gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTTGATTTAAAAAAATAAATTAATTAATTTTTTTTAATTATAGAG‑ATaaag                                                                                                      >  1:3135745/1‑148 (MQ=255)
                                                gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATTAAAATATTTTTTTTTAAATATAGAG‑ATaaag                                                                                                      >  2:1403288/1‑148 (MQ=255)
                                                gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACg                                                                                                      <  1:4165932/150‑1 (MQ=255)
                                                 tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGa                                                                                                     <  1:3405943/150‑1 (MQ=255)
                                                  gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAGTTTTGAAAAAATAAATAAAATATTTTTTTTTTTATATAGTG‑GTAACGAt                                                                                                    >  1:3716891/1‑150 (MQ=255)
                                                   aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATc                                                                                                   <  2:3172288/150‑1 (MQ=255)
                                                   aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATc                                                                                                   <  2:2250553/150‑1 (MQ=255)
                                                    atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATca                                                                                                  <  2:3475352/150‑1 (MQ=255)
                                                        tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAAATATTTGTTTTTAAATATAGAG‑AGAACGATCACaaa                                                                                              >  2:2821439/1‑150 (MQ=255)
                                                            tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACg                                                                                          <  2:3236417/150‑1 (MQ=255)
                                                            tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAGATTTAAAAATATAATTTAATTATTTTTTTTTTAATATAGAG‑ATAAATATaaaaaaaaag                                                                                          >  2:639737/1‑148 (MQ=255)
                                                             gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGa                                                                                         <  2:2101935/150‑1 (MQ=255)
                                                                 gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGGGTTTAAAAAAATAAATAAAATTTTTTTTTTTTAATATATAG‑ATAAAGATaaaaaaaaacaccaa                                                                                     >  1:3320505/1‑143 (MQ=255)
                                                                    ccGCATCACCGCCGGGCTGTGGGTTTTTCTGCTTGTGCTTTGTCTATACAACTTAAATTTTAATATCCTGTCTT‑TTGTTTTCTTTTTAAAAATATAATTAATTTTTTTTTTTTTAAATATCGAT‑TTAACTATCACAAAAACGACAATATg                                                                                  <  2:4105296/150‑1 (MQ=255)
                                                                      gCATAACCGCGGCGCTGTGCTTTGTTCAGCTTCTGGTTTGTCTATACCACTTACTTTTTCATATCCTGTCGT‑TTTTTTTCGTTTTCAAAATATATTGAAATTATTTTTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGaa                                                                                <  2:3872800/150‑1 (MQ=255)
                                                                      gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGT‑GTGTTTTCGATTTAAAAATATAATGAAAATATTTTTTTTTAAATATATAGTATAA‑GATACCAAAAACAACAAAATGaa                                                                                >  2:2607395/1‑150 (MQ=255)
                                                                         tacccgcggCGCGGTGCTTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTGGT‑TTGTTTTCTATTAAAAAATATAATTAATTTTTTTTTTTTTAAATATCTTG‑ATAACGATAAAAAACACGACAATATGAAAat                                                                             <  1:4208005/147‑1 (MQ=255)
                                                                         tAACCGCCGGGGTGTGCGTTGTTAAGCTTCTGGTTTGTCTATACCACGTAAATTTTCATATCATGTCGT‑TTGTTTTCTATTTAAAATTATAATTAAATTTTTTTTTTTTAAATTTCTAT‑ATAACGATCACAAAAACGACAATATGAAAat                                                                             <  2:4041539/150‑1 (MQ=255)
                                                                         tAACCGCCGCGCTGTGGGTTTTTCAGCATTTGCTTTGTCGATACCCCTTAAATTTTCATATCCTGTGTT‑TTGTTTTCGATTAAAAAATATATTTAATTTTTTTTTTTTTAAATATCTAT‑ATAACGATCACAAAAACGACAATATGAAAat                                                                             <  2:2181803/150‑1 (MQ=255)
                                                                          aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGGGTTTAAAAAAAAAATTTAATTATTTTTTTTTAAATATAGAG‑GTTACGAACACAAAAAAGACAAAATGagaaat                                                                            >  1:3572728/1‑148 (MQ=255)
                                                                          aaCCGCCGCGCTGTGCGTTGTCCAGCATCTGCTTTGTCTATACCACGTACTTTTAAATATCCTTTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAatt                                                                            <  2:4335389/150‑1 (MQ=255)
                                                                           aCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGGTTTGGATTTAAAAATATAATTAAAATATTTTTTTTTTAAAATAGAG‑ATGACGATCACAAAAACAACAAAATGAAAaata                                                                           >  1:2089058/1‑150 (MQ=255)
                                                                              gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTc                                                                        <  2:1423091/150‑1 (MQ=255)
                                                                                    cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTTTTTTGGATTTAAAAATATAATTAAAATATTTTTTTTTTAAAATAGCG‑ATAAAGATAAAAAAAAACAAAAAATTAAAAATATTAgaggag                                                                  >  1:2512430/1‑150 (MQ=255)
                                                                                    cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GGGTTTTTTTTTTTTAAATATAATTTAAATATTTTTTTTTTTATATTAAG‑ATAAAGATCAAAAAAAAAACAAAAATAAAaatattattggtg                                                                  >  1:2944850/1‑143 (MQ=255)
                                                                                     tgtgCGTTGTACACCATCTGCTTTTTCCACACCACTAACATTTTCATACCCTGTCTT‑TTTTTTTCTATTAAAAAATATAATTAATTTATTTTTTTTTAAATATCGAG‑AAAACTAACACAAACACGACAATATGAAAATTATTCGAGGAGt                                                                 <  1:1222719/150‑1 (MQ=255)
                                                                                      gtgCGTTGTTCAGCATCTGCTTGGTCGATACCACGTACATTTTCATATCCGGTCGT‑TTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTg                                                                <  2:1351616/150‑1 (MQ=255)
                                                                                       tgCGTTGTTCAGCATCTGCTTGGTCGATACCACGTACATTTTCATATCCTGGCGT‑TTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGa                                                               <  2:1010578/150‑1 (MQ=255)
                                                                                             gttCAGCATCTGCTTTGTCTATACCCCGTAAATTTTCATATCCTGTCGT‑TTGTTTTCTATTTCAAAATATAATGAATTTATTTTTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCa                                                         <  2:3556273/150‑1 (MQ=255)
                                                                                             gttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTTTTTTAGATTTAAAAAAAAAAATAAAATATTTTTTTTTAAATATCGAG‑ATAACGATAACAAAAAAGAAAAAAAGAAAAATATTAGAGGAGTTGAAGGCa                                                         >  2:1692722/1‑150 (MQ=255)
                                                                                              ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGGATTTAAAAAAAAAATTAAAAAAATTTTTTTTTAATATAGAG‑AAAAACAAAACAAAAAAAACAAAAAGAAAATTAATAGAGGACTGTAAGGCaa                                                        >  1:1366525/1‑150 (MQ=255)
                                                                                               tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTAGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATAGAG‑ATAACGAAAACAAAAAAGACAAAATTAAAATTATTAGTGGGTTGAAAAGCaaa                                                       >  2:1595012/1‑150 (MQ=255)
                                                                                                  tcATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCTT‑TTGTTTTCTATTACAAAATATAATTAAATTATTTTTTTTTAAATATCGAG‑ATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaa                                                    <  2:2997888/149‑1 (MQ=255)
                                                                                                               cGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGATTTAAAAATATAATTAAATTATTTTTTTTTTAATATCGAG‑ATAAAGATAACAAAAAAGACAATATTAAAATTATTGGAGGAGTGTAAGGGAAAAAAAAGGCCTCCCGaa                                       >  2:3568260/1‑149 (MQ=255)
                                                                                                               cGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGGATTTCAAAATATAATGAAAATATTTTTTTTTAAATATCGAG‑TTAACGATCACAAAAACGACACTATGAAAATTATTAGAGGGGTGAAAGGCCAAAAAACCGCCACCCGag                                       >  2:1933523/1‑149 (MQ=255)
                                                                                                                   aCCACGTACATTTTCATATCCTGTCGT‑GTGTTTTTGATTTGAAAATATAATTAAAATATTTTTTTTTTAATTTAGAG‑TTTACGATAACCAAAACGACAATATTAAAATTTTTAGGGGGGGGTAATGGAAAAAAAAGGCCTGCCGCTTggg                                   >  2:4163751/1‑150 (MQ=255)
                                                                                                                   aCCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGGTTTTGAAAAAATAATTTAAATATTTTTTTTTTAATATGGAG‑ATTAACATGACAAAAAAGACCAAATTAAAATTATTTGGGGGGGGTAATGCCAAAAAAAGGCCGCCCGGTTggg                                   >  2:571123/1‑150 (MQ=255)
                                                                                                                     cACTTACTTTTTCTTATCTTGTCTT‑TTTTTTTCTTTTTAAAAATATAATTAATTTTTTTTTTTTTAAATATCTTG‑ATACCGAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                 <  1:1015078/150‑1 (MQ=255)
                                                                                                                     cACGTAAATTTTCATATCCTGTCTT‑TTTTTTTATATTTAAAAATATAATTAAATTATTTTTTTTTAAATATCTAT‑ATAACTAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                 <  1:320681/150‑1 (MQ=255)
                                                                                                                      aCGTACATTTTCATATCCTGTCGT‑GTGTTTTAGCTTTAAAAAAATAATGAAATTATTTGTTTTTTAATATCGAG‑ATTACGATAACAAAAACGACAATATGACAATTATTGGAGGAGTGGAAGGCACAAAAAACGCCACCCGCTAGGGGAg                                >  2:2767416/1‑150 (MQ=255)
                                                                                                                       cGTACATTTTCATATCCTGTCGTGTTGTTTT‑GATTTAAAAATAAAATGAAAATATTTTTTTTTTAAAATAGAG‑ATAACGATAAAAAAAAACACCAAAATAAAATTATTAGGGGTGTGGAAGGCAAAAAAAACGCCACCCCAGAGGGGAGc                               >  2:780183/1‑150 (MQ=255)
                                                                                                                        gTACATTTTAAGACCCTGTCTT‑TTGTTTTCGATTCAAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                              <  2:2212713/150‑1 (MQ=255)
                                                                                                                           cATTTTCATATCCTGTCGT‑GTGTTTTGGATTTACAAAAAAAATTAAAATATTTTTTTTTTAATTTAGAG‑ATAACGATCAAAAAAAATACAATATTAAAATTATTAGTGGGGTTTAAAGGAAAAAAAAAGCCGCCCCCTAGGGGCGCCgga                           >  2:1187951/1‑148 (MQ=255)
                                                                                                                            tttttCATATCCTGGGGT‑TTTTTTGCGATTTCAAAATATAATTAATTTTTTTTTTTTTAAATTTCGAT‑ATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                          <  1:126072/149‑1 (MQ=255)
                                                                                                                               ttCATACCCTTTCTT‑TTTTTTTCTTTTTAAAAATATAATTAAATTTTTTTTTTTTAAATATCTAT‑ATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaa                       <  1:272193/150‑1 (MQ=255)
                                                                                                                                         gTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGAAAATATGAAAATTATTCGAGTTGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtg             >  2:3891515/1‑150 (MQ=255)
                                                                                                                                           tgt‑ttgtttTCTATTTAAAAATATAATTAAATTATTTGTTTTTAAATATCGAT‑ATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt           <  2:4038696/149‑1 (MQ=255)
                                                                                                                                           cGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt           >  1:3505448/1‑150 (MQ=255)
                                                                                                                                           cGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt           >  1:2076723/1‑150 (MQ=255)
                                                                                                                                            gt‑ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt          >  1:945345/1‑150 (MQ=255)
                                                                                                                                             t‑ttgtttTCGATATCAAAAAATAATGAAAATATTTGTTTTTAAATAACGAG‑ATAACGATCACAAAAACGACAATATGAAAATAATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCCATATGGAAGCCGTAGCAAAGAGCGAGTGttat         >  1:3239958/1‑148 (MQ=255)
                                                                                                                                               ttgtttTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt        <  2:3629053/150‑1 (MQ=255)
                                                                                                                                                tgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa       >  2:3130310/1‑150 (MQ=255)
                                                                                                                                                 gtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt      >  2:1259770/1‑150 (MQ=255)
                                                                                                                                                    ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAGATTATTGGTGGAGTGTAAGGCAATAAAACGGCGTCGGGATGGGGTAGCCGTAGCTAGGTGCGGGGGtttgtttggg   >  1:2194285/1‑143 (MQ=255)
                                                                                                                                                    ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAACGTGCGCGTGTTTTTATGcc   >  1:1704703/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
ATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCG  >  CP009273/2170558‑2170855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A