Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,707 C→A 19.9% intergenic (‑24/+284) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7070CA19.9% 252.4 / 4.4 122intergenic (‑24/+284)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (16/67);  new base A (20/2);  total (53/69)
Fisher's exact test for biased strand distribution p-value = 7.19e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

ATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCG  >  CP009273/2170558‑2170855
                                                                                                                                                     |                                                                                                                                                         
aTGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTc                                                                                                                                                           <  2:3484514/150‑1 (MQ=255)
 tGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCg                                                                                                                                                          <  2:4072070/150‑1 (MQ=255)
   cAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATCCCACGTACATTTTCATATCCTGTCGTGTGTTTTAGAt                                                                                                                                                        >  1:997528/1‑150 (MQ=255)
    aGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGAtt                                                                                                                                                       >  2:978646/1‑150 (MQ=255)
    aGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAtt                                                                                                                                                       <  1:1104279/150‑1 (MQ=255)
    aGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAtt                                                                                                                                                       <  1:2576261/150‑1 (MQ=255)
    aGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAtt                                                                                                                                                       <  1:2576244/150‑1 (MQ=255)
    aGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAtt                                                                                                                                                       <  1:1105191/150‑1 (MQ=255)
    aGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGAtt                                                                                                                                                       >  2:2755094/1‑150 (MQ=255)
     ggTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAttt                                                                                                                                                      <  2:110504/150‑1 (MQ=255)
     ggTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAttt                                                                                                                                                      <  2:1447028/150‑1 (MQ=255)
       ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                    <  1:4177878/150‑1 (MQ=255)
        tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaa                                                                                                                                                   <  1:4429772/150‑1 (MQ=255)
        tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTGTTGTATTTaaa                                                                                                                                                   >  1:1244818/1‑150 (MQ=255)
        tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTGTTGGTTTTTaa                                                                                                                                                   >  1:1246922/1‑150 (MQ=255)
           cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTTTATTTAaaaaa                                                                                                                                                >  1:633875/1‑149 (MQ=255)
           cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAat                                                                                                                                                <  1:4320030/150‑1 (MQ=255)
           cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGTTTTCAAAat                                                                                                                                                >  1:633777/1‑150 (MQ=255)
           cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAat                                                                                                                                                >  1:3070763/1‑150 (MQ=255)
            aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAata                                                                                                                                               <  1:724721/150‑1 (MQ=255)
              cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtat‑a                                                                                                                                            <  2:3713627/150‑1 (MQ=255)
               ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑aa                                                                                                                                           <  1:3718690/150‑1 (MQ=255)
                gTTTCTACCACCACTTGCATCGGTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAt                                                                                                                                          <  2:4414903/150‑1 (MQ=255)
                  ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑gt                                                                                                                                       <  2:967536/150‑2 (MQ=255)
                   tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑Gaa                                                                                                                                      <  2:1970251/150‑1 (MQ=255)
                    cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTGTTTAGATTTCAAAATAT‑aaataaa                                                                                                                                      >  2:747324/1‑145 (MQ=255)
                    cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGCTTTAAAAATAT‑AAT‑Gaaa                                                                                                                                     >  2:3123679/1‑150 (MQ=255)
                    cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAAT‑AAT‑Gaaa                                                                                                                                     >  2:3124616/1‑150 (MQ=255)
                         accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGGTTTAAAAATAA‑AAT‑TAAattatt                                                                                                                                >  1:1090930/1‑150 (MQ=255)
                           cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATAT‑AAT‑GAAAATAtttt                                                                                                                              >  2:1545274/1‑149 (MQ=255)
                            acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTTTTTTTAAAAAAAA‑AAT‑TAAAATAttttt                                                                                                                             >  1:2123853/1‑148 (MQ=255)
                            acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAAAAT‑AAT‑TAAATTAttttt                                                                                                                             >  1:3562270/1‑148 (MQ=255)
                            acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGAACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAAAAA‑AAA‑TAAAATAttttt                                                                                                                             >  1:3562281/1‑148 (MQ=255)
                               tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGtttt                                                                                                                          <  2:4463034/150‑1 (MQ=255)
                                 cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAA‑AAT‑TAAATTAAttttttttt                                                                                                                        >  2:292015/1‑149 (MQ=255)
                                  aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTaa                                                                                                                       >  2:2634303/1‑150 (MQ=255)
                                     gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAata                                                                                                                    <  2:2230287/150‑1 (MQ=255)
                                      tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAatat                                                                                                                   <  1:1887114/150‑1 (MQ=255)
                                      tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAatat                                                                                                                   <  1:620950/150‑1 (MQ=255)
                                         cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCga                                                                                                                <  2:3189806/150‑1 (MQ=255)
                                           agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAAT‑AAT‑GAAATTATTTGTTTTTAAATATCgaga                                                                                                              >  2:358376/1‑150 (MQ=255)
                                             aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑Ta                                                                                                           <  1:2403137/150‑1 (MQ=255)
                                             aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTGTTTGATTTTTAAAAATAAATATAAAATATTTTTTTTtatatagaaatt                                                                                                              >  1:2433680/1‑144 (MQ=255)
                                              ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑Taa                                                                                                          <  1:235210/150‑1 (MQ=255)
                                                gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACg                                                                                                        <  2:2785914/150‑1 (MQ=255)
                                                  gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACGAt                                                                                                      <  2:3959314/150‑1 (MQ=255)
                                                  gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACGAt                                                                                                      <  2:3959338/150‑1 (MQ=255)
                                                  gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAAT‑AAT‑GAAATTATTTTTTTTTAAATATAGAGA‑TAACGAt                                                                                                      >  2:1549737/1‑150 (MQ=255)
                                                  gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATAT‑AAA‑GTAATTATTTGTTTTTAAATATAGAGA‑TAAAGAt                                                                                                      >  2:2221263/1‑150 (MQ=255)
                                                    atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTTGTTTTGAAAATAT‑AAT‑TGAATTATTTGTTTTTTTATATAGAGA‑TAAAGAtaa                                                                                                    >  2:931090/1‑148 (MQ=255)
                                                    atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACGATca                                                                                                    <  1:3733324/150‑1 (MQ=255)
                                                       ttGAATGCCGGAACCGCATAACCGCCGCGCGGTGCGGTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTCAAAATAT‑AAA‑GAAATTATTTGTTTTTAAATATCGCGA‑TAACCATCACaa                                                                                                 >  2:2355151/1‑150 (MQ=255)
                                                        tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACGATCACaaa                                                                                                <  1:1889909/150‑1 (MQ=255)
                                                        tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTGAAAATAT‑AAT‑GAAAATATTTTTTTTTAAATATCGAGA‑TAAACATCACaaa                                                                                                >  2:3333638/1‑150 (MQ=255)
                                                         gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAAAAT‑AAA‑GAAATTATTTGTTTTTTAATAAAGAGA‑TAACGATCaaaaaa                                                                                               >  2:92484/1‑150 (MQ=255)
                                                          aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACGATCACaaaaa                                                                                              <  2:4340816/150‑1 (MQ=255)
                                                           aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACGATCACAAAAAc                                                                                             <  1:4216316/150‑1 (MQ=255)
                                                            tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACGATCACAAAAACg                                                                                            <  1:1163110/150‑1 (MQ=255)
                                                            tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACGATCACAAAAACg                                                                                            <  1:1163127/150‑1 (MQ=255)
                                                            tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACGATCACAAAAACg                                                                                            <  1:912793/150‑1 (MQ=255)
                                                             gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTTAAAAAAA‑AAT‑TTAAATTTTTTTTTTTTAATATATTGA‑TAAAGATaaaaaaaaaca                                                                                           >  1:2289231/1‑147 (MQ=255)
                                                             gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTTAAAAATAT‑AAT‑TTAATTATTTGTTTTTTTATATAGATA‑TAACAATaaaaaaaaaaa                                                                                           >  1:2289194/1‑147 (MQ=255)
                                                              ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACGATCACAAAAACGAc                                                                                          <  1:3960572/150‑1 (MQ=255)
                                                              ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAT‑AAT‑GAAATTATTTTTTTTTAAATATAGAGA‑TAAAGATCACAAAAAAGAc                                                                                          >  2:747405/1‑150 (MQ=255)
                                                                       cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAA‑GAAATTATTTGTTTTTTAATATAGAGA‑TAACGATAACAAaaaagaaaaaaagaaa                                                                                 >  2:3081464/1‑150 (MQ=255)
                                                                         tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATAT‑AAT‑GAAATTATTTTTTTTTAAATATCGTGA‑TAACGATCACAAAAACGACAATATGAAAat                                                                               <  2:1569744/150‑1 (MQ=255)
                                                                              gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATAT‑AAT‑TAAATTATTTTTTTTTAAATATAGAGA‑TAACGATCAAAAAAAAGACAAAATGAAAattatta                                                                          >  2:3621279/1‑149 (MQ=255)
                                                                               ccgcGCTGTGCGTTGTACAGCACCTGCTTTGTCGATCCCACGTACATTTTCAAAACCTGTCGTTTGTTTTCGATTACAAAAAAT‑AAT‑CAAATTATTTTTTTTTAAATATCTATA‑AAACGATCACAAAAACGACAATATGAAAATTATTCg                                                                         <  2:1008196/150‑1 (MQ=255)
                                                                                  cgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATAA‑AAA‑GAAATTATTTTTTTTTTAATATAGAGA‑TAAAGATCACAAAAAAGACAAAATGAAAAATATTAgagg                                                                      >  2:1820085/1‑150 (MQ=255)
                                                                                  cgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAATAT‑AAT‑GAAATTATTTGTTTTTTAATATCGAGA‑TAACGATAACAAAAAAGACAAAATTAAAAATATTAgagg                                                                      >  2:1819889/1‑150 (MQ=255)
                                                                                    cTGTGGGTTGTTCAGCATCTGCTTTGTCGAACCCACTTACATTTACAAATCCTGTCGTTTTTTTTCTATTAAAAAATAT‑AAT‑CAATTTATTTTTTTTTAAATATCTAGA‑TAACGACCACAAACACGACAATATGAAAATTATTCgaggag                                                                    <  1:964353/150‑1 (MQ=255)
                                                                                     tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTCAAAATAT‑AAA‑GAAATTATTTGTTTTTAAATATAGAGA‑TAACGATAACAAAAAACACAAAATTAAAATTATTAGAGgtgt                                                                   >  2:1853524/1‑150 (MQ=255)
                                                                                      ttgggttgtgCAGCATCTGCTTTTTCGATACAACGTACATTTTCATATCCTGTCGTTTTTTTTATTTTTCAAAATAT‑AAT‑TCAATTTTTTGTTTTTAAATATCTTGA‑TAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTg                                                                  <  2:3091331/146‑1 (MQ=255)
                                                                                      gtgCGTTGTTCAGCATCTGCTTTGTCGATCCCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTACAAAATAT‑AAT‑CAAATTATTTTTTTTTAAATATCGCTA‑TAACTAACACAAACACGACAATATGAAAATTATTCGAGGAGTg                                                                  <  1:2326414/150‑1 (MQ=255)
                                                                                           ttgttCAGCATCTGCTTTGTCTATACCACTTACATTTACATATCCTGTCGTTTTTTTTCTATTACAAAATAT‑ATT‑TAATTTATTTTTTTTTAAATATCGAGA‑TAACTAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAgg                                                             <  1:1167120/150‑1 (MQ=255)
                                                                                           ttgtaCAGCATCTGCTTTGTCTATACCACTAACATTTTCATATCCTGTGGTTTGTTTTCTATTACAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAgg                                                             <  1:1167065/150‑1 (MQ=255)
                                                                                            tgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGTTTTGAAAAAAT‑AAT‑TTAATTATTTTTTTTTTAAAATAGTGA‑TTAAGATAACAAAAAAGACAAAATGAAAAATATTAGAGGAGtttaatgg                                                            >  1:3107284/1‑143 (MQ=255)
                                                                                                   cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGTTTTAAAAAAATAAAT‑AAAATAATTTTTTTTTAAA‑ATAGAGA‑AAAAGATAAAAAAAAAAACAAAAATAAAAAAATTATAGGAGTGTAATTG‑‑aaaaaaa                                                   >  1:2125116/1‑150 (MQ=255)
                                                                                                     tgtGCTTTGTCTATACCACGTAAATTTTCATATCCTGTCGTTTTTTTTCTATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGATA‑TAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACg                                                 <  2:2564844/148‑1 (MQ=255)
                                                                                                      cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCTTTTGTTTTCTATTACAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGATA‑TAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACgg                                                <  2:2509203/150‑1 (MQ=255)
                                                                                                             gTCGATACCACGTACTTTTTCATATCCTGTCTTTTGTTTTCTATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCg                                         <  2:1121703/150‑1 (MQ=255)
                                                                                                             gTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑TAAATTATTTGTTTTTAAATATCGAGA‑TAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCg                                         <  2:1121739/150‑1 (MQ=255)
                                                                                                              tCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATAA‑AAT‑GAAATTATTTTTTTTTAAATATAGAGA‑TAACGATAAAAAAAAAGACAAAATGAAAATTATTAGAGGAGTGAAAGGC‑‑AAAAAAAAGGCCTCCCGa                                        >  2:2717329/1‑150 (MQ=255)
                                                                                                                   aCCACGTACATTTTCATATCCTGTCGTGTTTTTTCGTTTTAAAAATAT‑AAT‑GAAATTATTTTTTTTTAAATATAGAGT‑TAACGATAACAAAAAAGAAAATATGAAAATTATTAGAGGTGTGAAAGGC‑‑ACAAAAAAGGCCTCCCGCTAggg                                   >  2:2784512/1‑150 (MQ=255)
                                                                                                                      acttACTTTTTCATAGCCTGTCTTTTGTTTTCTATTTCAAAATAT‑AAT‑GAAATTATTTTTTTTTAAATATCGATA‑TAACTATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAg                                <  1:700624/147‑1 (MQ=255)
                                                                                                                       cGTACATTTTCATTTCATGTCGTTTTTTTTCGATTTAAAAATAT‑AAT‑GAAATTTTTTTTTTTTAAATATCTAGA‑AAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGc                               <  2:4154047/150‑1 (MQ=255)
                                                                                                                          aCATTTTCATATCCTGTCGTGTGTTTTAGGTTTAAAAAAAT‑AAA‑TAAAATATTTTTTTTTTAATATAGATA‑TAAAGAAAACAAAAAAAAAAAAATTAAAATTAATCGATGGGGGAAATTC‑‑AAAAAAAAAGCCACCCGAAAGGGTAgacct                            >  2:2912458/1‑146 (MQ=255)
                                                                                                                           cATTTTCATATCCTGTCGTGTGTTTTGGTTTTTAAAAAAT‑AAA‑GAAAATATTTTTTTTTTAATATAGAGAGTAAC‑AAAACAAAAAAGACAAAATTAAAAGTATTAGAGGGGTGAAAGGC‑‑AAAAAAAACGCCTCCCGCTCGGGTAGCCggt                           >  2:4670805/1‑148 (MQ=255)
                                                                                                                           cATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAATAA‑AAA‑GAAAATAATTGTTTTTTAATATCGAGA‑TAAAGATAACAAAAAACAAAAAATGAAAAATATTAGAGGAGTGGAAGGC‑‑CAAAAAAACGCCACCCCCTAAGGCAGCCgaa                           >  2:4670986/1‑148 (MQ=255)
                                                                                                                            aTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                          <  2:166792/150‑1 (MQ=255)
                                                                                                                            aTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                          <  2:4418954/150‑1 (MQ=255)
                                                                                                                                            tttttttttAGTTTTAAAAATTT‑AAT‑GAAATTTTTTTTTTTTAAATATCTATA‑TAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt          <  1:3988626/149‑1 (MQ=255)
                                                                                                                                             tttgtttTCTATTTAAAAATAT‑AAT‑TAAATTATTTGTTTTTAAATATCGATA‑TAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt         <  1:1545274/150‑1 (MQ=255)
                                                                                                                                               ttttttCTATTTCAAAATAT‑AAT‑TAATTTTTTTTTTTTTAAATATCGATA‑TAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa       <  1:292015/148‑1 (MQ=255)
                                                                                                                                               ttttttCTATTACAAAATAT‑AAT‑TAAATTATTTGTTTTTAAATATCGAGA‑TAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa       <  2:1497114/148‑1 (MQ=255)
                                                                                                                                                 ttttCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa       >  2:3314608/1‑150 (MQ=255)
                                                                                                                                                 ttttCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCA‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt      >  2:3314736/1‑150 (MQ=255)
                                                                                                                                                 ttttCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCA‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGGGTTTTTAt      >  2:3314540/1‑150 (MQ=255)
                                                                                                                                                    tCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGATA‑TAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg  <  2:2816374/150‑1 (MQ=255)
                                                                                                                                                    tCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGAGAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg  >  2:3637939/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                         
ATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGA‑TAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCG  >  CP009273/2170558‑2170855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A