Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,707 C→A 20.7% intergenic (‑24/+284) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7070CA20.7% 457.9 / 17.6 236intergenic (‑24/+284)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (39/120);  new base A (45/3);  total (112/124)
Fisher's exact test for biased strand distribution p-value = 1.32e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

TGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCG  >  CP009273/2170559‑2170855
                                                                                                                                                    |                                                                                                                                                     
tGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTc                                                                                                                                                       <  2:1201423/149‑1 (MQ=255)
   aGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGAt                                                                                                                                                    >  2:754862/1‑149 (MQ=255)
    ggTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAtt                                                                                                                                                   <  2:1873312/149‑1 (MQ=255)
    ggTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAtt                                                                                                                                                   <  1:2981496/149‑1 (MQ=255)
    ggTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAtt                                                                                                                                                   <  2:3148054/149‑1 (MQ=255)
       tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑Ca                                                                                                                                               <  1:3467949/149‑1 (MQ=255)
        ggCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑Caa                                                                                                                                              <  1:758986/149‑1 (MQ=255)
          cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑Caaaa                                                                                                                                            <  1:133867/149‑1 (MQ=255)
           aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGAGTT‑Gaaaaa                                                                                                                                           >  1:944690/1‑148 (MQ=255)
               gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTT‑Aaaaaaaaaa                                                                                                                                       >  1:3265487/1‑144 (MQ=255)
                tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAt                                                                                                                                      >  2:3192350/1‑149 (MQ=255)
                 ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATg                                                                                                                                     <  2:3607135/149‑1 (MQ=255)
                 ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATg                                                                                                                                     <  1:4534649/149‑1 (MQ=255)
                 ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTT‑GAAAAAATTAtt                                                                                                                                     >  1:2733901/1‑148 (MQ=255)
                   cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGaa                                                                                                                                   <  2:1400569/149‑1 (MQ=255)
                    tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGaaa                                                                                                                                  <  2:1905461/149‑1 (MQ=255)
                    tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAAAATAAAGaaa                                                                                                                                  >  2:1711302/1‑149 (MQ=255)
                    tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTT‑AAAAAAATAAAGaaa                                                                                                                                  >  2:109543/1‑149 (MQ=255)
                       caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTTGATTT‑AAAAAAATAATTAAaata                                                                                                                               >  1:1043749/1‑149 (MQ=255)
                         ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAattatt                                                                                                                             <  2:781887/149‑1 (MQ=255)
                          cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTT‑GAAAATATAATGAAATTAttt                                                                                                                            >  2:1251018/1‑149 (MQ=255)
                            cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGt                                                                                                                          <  2:3719644/149‑1 (MQ=255)
                               gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGtttt                                                                                                                       <  1:1019995/149‑1 (MQ=255)
                               gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTT‑CAAAATATAATTAAATTAtttttttt                                                                                                                       >  1:2293514/1‑149 (MQ=255)
                                cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGAATT‑CAAAAAATAAATAAAATAAtttttttt                                                                                                                      >  1:3842091/1‑149 (MQ=255)
                                 aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTa                                                                                                                     >  2:4277941/1‑149 (MQ=255)
                                 aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTa                                                                                                                     <  2:4507974/149‑1 (MQ=255)
                                    gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAAAAAAAAGAAATTATTTTTTTTTAAat                                                                                                                  >  1:1990323/1‑149 (MQ=255)
                                    gTTTCGAGAGTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAat                                                                                                                  <  2:3661378/149‑1 (MQ=255)
                                     tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑AAAAAAATAATTAAAATAATTTTTTTTaaaaa                                                                                                                 >  2:4239872/1‑147 (MQ=255)
                                     tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTT‑AAAAATAAAATTAAAATAATTTTTTTTAAata                                                                                                                 >  1:182572/1‑149 (MQ=255)
                                      ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                <  1:4015571/149‑1 (MQ=255)
                                      ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTT‑CAAAATAAAAAGAAATTATTTTTTTTTAAatat                                                                                                                >  2:107800/1‑149 (MQ=255)
                                       tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                               <  1:3186788/149‑1 (MQ=255)
                                       tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGAATT‑AAAAAAATAAATAAATTATTTTTTTTTTAaaata                                                                                                               >  1:3536648/1‑148 (MQ=255)
                                         gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                             <  1:3207778/149‑1 (MQ=255)
                                         gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                             <  2:3219211/149‑1 (MQ=255)
                                          agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTT‑CAAAATATAAATTAAATATTTTTTTTTAAATATagag                                                                                                            >  2:4612606/1‑149 (MQ=255)
                                           gtttGTGTATATTGAATGCCGGAACCGCATAACCTCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTAGATACCACGTACATTTTCAGATCCTGTCGTTTGTTTTCGATTT‑CAAAATATATTGAAATTATTTGTTTTTATATTTCgaga                                                                                                           <  2:2419702/147‑1 (MQ=255)
                                            aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGAt                                                                                                          <  1:1699283/149‑1 (MQ=255)
                                            aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTT‑CAAAATAAAAAGAAAATATTTTTTTTTTAataaaaaggt                                                                                                          >  1:2541929/1‑142 (MQ=255)
                                             ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑AAAAATATAATTAAAATATTTGTTTTTAAATATAGAGATa                                                                                                         >  2:330093/1‑149 (MQ=255)
                                              tgtgAATATTGAATGCCGGAACCGCATATCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATaa                                                                                                        <  2:3335681/149‑1 (MQ=255)
                                              tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTGTTAGATTT‑AAAAAAATAATGAAATTATTTGTTTTTATATATCgagctca                                                                                                        >  1:3729132/1‑145 (MQ=255)
                                                tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGATTT‑AAAAATAAAAAGAAAATAATTTTTTTTTAATAAAGAGTTaaac                                                                                                      >  1:656431/1‑147 (MQ=255)
                                                tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTTTGATTT‑GAAAATATAAATAAAATATTTTTTTTTTAATATAGAGaaaaaa                                                                                                      >  1:2407081/1‑147 (MQ=255)
                                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                     <  1:2242559/149‑1 (MQ=255)
                                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                    <  2:1134591/149‑1 (MQ=255)
                                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                    <  2:2497768/149‑1 (MQ=255)
                                                   agatTGAATGCCGGATCCGCATAACCGCCGCTCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                   <  2:4430435/147‑1 (MQ=255)
                                                    tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGGAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                  <  2:3268497/149‑1 (MQ=255)
                                                    tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                  <  2:2523449/149‑1 (MQ=255)
                                                     atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAAATATTTTTTTTTAAATATCGAGATAACGATcac                                                                                                 >  2:3961936/1‑149 (MQ=255)
                                                      ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTT‑CAAAATATTATTAAATTATTTTTTTTTTAATATAGAGATAACGATaaca                                                                                                >  2:561847/1‑149 (MQ=255)
                                                          aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                            <  1:2944084/149‑1 (MQ=255)
                                                           tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACAATCACAAAAAc                                                                                           <  2:960173/149‑1 (MQ=255)
                                                             ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                         <  2:1180037/149‑1 (MQ=255)
                                                               ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTATATTT‑AAAAATATAAATTAAATATTTTTTTTTAAAAATAGAGATAAAGATCACaaaaaacaca                                                                                       >  1:1145551/1‑144 (MQ=255)
                                                               ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGTTTT‑TAAAATATAATTTAATTATTTTTTTTTTAATATATGGATAAAGATAACAAAAAAGACa                                                                                       >  1:500061/1‑149 (MQ=255)
                                                                 aaCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTT‑GAAAATATAATGTAAATATTTTTTTTTAAAAATAGTGATTAAGATAACCAAAACGACaaa                                                                                     >  1:4285796/1‑148 (MQ=255)
                                                                  acctaaTAACCGCCGCGGTGTGTTTTGTTCTGCTTGTGATTTGTCTATACCACTAACTTTTTAATTTCCTGTGTTTTTTTTTGGTTTT‑AAAAATATAATTAATTTTTTTTTTTTTAATTATGTATATAACGATCACAAAAACGACAata                                                                                    <  2:4106002/144‑1 (MQ=255)
                                                                  aCCGAATAACCGCCGCGGTGTGGTTTGTTCATCATCTGCTTTGTCTATACCACTTACTTTTTAATATCGTGTGGTTTTTTTTCTTTTT‑CAAAATATAATTAAATTATTTGTTTTTAAATATCGATTTAACGAACACAAAAACGACAata                                                                                    <  2:3367397/149‑1 (MQ=255)
                                                                   ccGCATAACCGCCGCGCTGTGGGTTGTTCAGCATCTGATTTGTCTATACCACTTACTTTTTCATATCCTGTGGTTTGTTTTCTATTT‑CAAAATATAATGAATTTTTTTGTTTTTAATTTTCGAGATAACGATCAAAAACACGACAatat                                                                                   <  2:92515/149‑1 (MQ=255)
                                                                       aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTGGATTT‑CAAAAAATAATGAAAATATTTGTTTTTATATATCGAGTTCACGATCACAAAAACAACACAATGaaa                                                                               >  1:2644384/1‑149 (MQ=255)
                                                                        tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCCCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaaaa                                                                              <  2:3569459/149‑1 (MQ=255)
                                                                        tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTT‑CAAAATATAATGAAAATATTTTTTTTTAAAAATCGAGATAACGAACAAAAAAAACACAAAAAGaaaa                                                                              >  2:4100214/1‑149 (MQ=255)
                                                                           ccgccgGGATGTGCTTTTTTCAGCATCTGATTTTTATATACCACTTACTTTTTAATATCCTGTTGTTTTTTTTCTTTTA‑CAAAATTTATTGAAATTATTTGTTTTTAAATATCTATAAAACGATCAAAAAAACGACAATATGAAAatta                                                                           <  2:1333494/149‑1 (MQ=255)
                                                                           ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTTTCTTTTGTTTTCGATTT‑CAAAATATAATTAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAatta                                                                           <  1:1969566/149‑1 (MQ=255)
                                                                           ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTT‑CAAAAAATAAATAAAATATTTTTTTTTAAATATAGAGATAACGATAACCAAAACGCCAATATgcacaata                                                                           >  1:1540573/1‑142 (MQ=255)
                                                                             gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTTTTTT‑AAAAAAATAATTTAAATTTTTTTTTTTTAATAAAGATTTTTAGAAGACAAAAACAACAAAATGTTAattatt                                                                         >  1:3710217/1‑149 (MQ=255)
                                                                                  gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGTTTT‑CTAAAAATAATGAAAATATTTTTTTTTTAAAATAGAGTTAAAGATAACAAAAAAACCAAAATTGAAATTATTCtagg                                                                    >  2:805991/1‑149 (MQ=255)
                                                                                  gcTGGGCTTTTTGCAGCATCTGCTTTGTCGATACCCGGAACTTTTTCATATCCTGTCGTTTGTTTTCGATTT‑AAAAATATAATCAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCgagg                                                                    <  2:567124/149‑1 (MQ=255)
                                                                                      tgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGATTTAAAAAAAAAAATAAAATTATTT‑TTTTTTTATATAGAGTTAACGGAAACAAAAAAGACAAGATGAAAATTATTAgagggttg                                                                >  1:2690459/1‑145 (MQ=255)
                                                                                              tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTT‑AAAAAAATAATGAAATTATTTTTTTTTAAATATAGAGATAAACATAACAAAAACGACAAAATGAAAATTATTAGAGGAGTGGAAGGCaa                                                        >  2:4264202/1‑149 (MQ=255)
                                                                                               cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTGGATTT‑CAAAATATAATTAAATTATTTTTTTTTTAATATATAGATAAAGATAAAAAAAAAGACAAAATTAAAAATATTAGAGGAGTGTAAGTCaaa                                                       >  2:2166140/1‑149 (MQ=255)
                                                                                                aGCATCTGCTTTGTCGATACCACTTACATTTTCATATCCTGTCTTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaa                                                      <  2:2552036/149‑1 (MQ=255)
                                                                                                   aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTTGATTT‑AAAAAAAAAATTAAAATATTTTTTTTTTAATATAGAGTTAAAGATCACAAAAAAGAAAATATTAAAAATATTATATGAGTGAAAGGCaaaaaaa                                                   >  1:1080140/1‑149 (MQ=255)
                                                                                                   aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTGGATTT‑GAAAAAAAAATTAAAAAATTTTTTTTTTAATATAGCGAAAACCATAACAAAAACAAAAAAAATAAAAAAATTAGAGGAGGGAAAAGCacaaaaa                                                   >  1:4651389/1‑149 (MQ=255)
                                                                                                    tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGCTTT‑GAAAAAATAATGTAAATATTTTTTTTTATATATCATGATTAAGATTACAAAAAAGACAAAATGAAAAATATTGGTTGTGTTTAAGGGaaaaaaaa                                                  >  2:2814639/1‑148 (MQ=255)
                                                                                                       gCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGTGTTTTCGATTT‑CAAAAAATAATGAAATTAATTGTTTTTAAATATCGAGATAACCATCACAAAAACCACCATATGAAAATTATTCGAGGAGGGAAATGCCAAAAAAAGGc                                               >  2:1973671/1‑149 (MQ=255)
                                                                                                         tttGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTTGTTTT‑AAAAATATAATTTAATTATTTTTTTTTTAATATAGATTTAAACATAAAAAAAAAGAAAATATTTAAATTATTATGGGAGTGGAATGCaaaaaaaaaggct                                             >  2:2080768/1‑143 (MQ=255)
                                                                                                              cGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTT‑AAAAAAAAAATGAAATTATTTTTTTTTTAATATAGAGAAAAAGATCAAAAAAAACACAAAATTAAAATTTTTAGAGGTGTGAAAGGCAAAAAAAAGGCCACCCGa                                        >  2:1421696/1‑149 (MQ=255)
                                                                                                               gATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTT‑AAAAATATAAATTAAATATTTTTTTTTTAATATATAGATAAAGAAAAAAAAAAACAAAAAAATAAAAATATTAGTGGTTTGAAAGGCAAAAAAAAGGccaacccat                                       >  1:1564670/1‑142 (MQ=255)
                                                                                                                  aCCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTT‑AAAAAAATAATTAAATTATTTTTTTTTAAATATAGAGATAAAGATCACAAAAACGACAATATTAAAATTATTAGAGGAGTGTAAGGCAAAAAAAAGGCCGCCCCAGAgg                                    >  2:1052992/1‑149 (MQ=255)
                                                                                                                       gTACATTTTCTTATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                               <  2:173982/149‑1 (MQ=255)
                                                                                                                         aCATTTTCATATCCTGTCGTTTTTTTTTGATTT‑TAAAATATAATGAAAATATTTTTTTTTAAATATAGAGATAAAGATCACAAAAAACACACTATTAAAATTTTTAGAGGGGTGTAATGCAAAAAAAATGCCGCCCCATATGGCAGccc                             >  2:1316994/1‑148 (MQ=255)
                                                                                                                           tttttCATATCCTTTCTTTTGTTTTATATTA‑AAAAATAAAATCAATTTATTTTTTTTTAAATATCTATATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                           <  1:3486001/148‑1 (MQ=255)
                                                                                                                           aTTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                           <  2:2954598/149‑1 (MQ=255)
                                                                                                                           aTTTTCATATCCTGTCGTTTGTTTTCGATTT‑AAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                           <  2:2734105/149‑1 (MQ=255)
                                                                                                                            ttttCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                          <  2:2648273/149‑1 (MQ=255)
                                                                                                                              ttCATATCCTGTCGTTTGTTTTCGATTT‑AAAAATATAATGAAATTTTTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCa                        <  1:394402/149‑1 (MQ=255)
                                                                                                                                         tCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATCGGGAAGCCGTAGCAAAGTGCGCgtg             >  2:2991625/1‑149 (MQ=255)
                                                                                                                                          cGTTTGTTTTCTATTT‑CAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt            <  2:1587178/149‑1 (MQ=255)
                                                                                                                                           gtttgtttTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTTTTCGAGGAGTGGAAGGCAAAATAACGGCCTCCCGAGCGGGGCGCCGTTGCAAAGTGCGCGGGtt           >  2:4509539/1‑149 (MQ=255)
                                                                                                                                           gtttgtttTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt           >  1:2299961/1‑149 (MQ=255)
                                                                                                                                            ttttttttCGATTA‑CAAAATATAATTAAATTATTTTTTTTTAAATATCTATATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt          <  1:4691992/149‑1 (MQ=255)
                                                                                                                                             ttgtttTCGTTTT‑AAAAATTTAATGAAATTTTTTTTTTTTAATTATCGTGATACGGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt         <  1:4167352/149‑1 (MQ=255)
                                                                                                                                               gtttTCGATTT‑CAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa       <  2:656431/149‑1 (MQ=255)
                                                                                                                                                    cGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGACAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg  >  1:2937838/1‑149 (MQ=255)
                                                                                                                                                    cGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAATAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg  >  2:3394810/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCG  >  CP009273/2170559‑2170855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A