Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,474,353 G→T 100% Q435K (CAG→AAG)  emrY ← putative multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,474,3530GT97.9% 801.5 / 5.6 251Q435K (CAG→AAG) emrYputative multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base T (129/116);  total (132/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 251 total aligned reads displayed.

CTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTA  >  CP009273/2474207‑2474499
                                                                                                                                                  |                                                                                                                                                  
ctcATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACt                                                                                                                                                 >  2:4606177/1‑150 (MQ=255)
  cATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTAt                                                                                                                                               >  2:1541723/1‑150 (MQ=255)
        cAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTa                                                                                                                                         >  1:3614890/1‑150 (MQ=255)
        cAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTa                                                                                                                                         >  1:3991270/1‑150 (MQ=255)
         aGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTaa                                                                                                                                        >  2:1710502/1‑150 (MQ=255)
         aGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATATCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTaa                                                                                                                                        >  2:589448/1‑150 (MQ=255)
           aaaTAGAAAGTGACTGCTGTGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACAATAATATATATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATTGTGTAACg                                                                                                                                      >  1:5058005/1‑150 (MQ=255)
            aaTAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGa                                                                                                                                     >  1:2414898/1‑150 (MQ=255)
            aaTAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGa                                                                                                                                     >  2:4073830/1‑150 (MQ=255)
            aaTAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGa                                                                                                                                     >  1:4437663/1‑150 (MQ=255)
             aTAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGAt                                                                                                                                    <  1:131346/150‑1 (MQ=255)
                gAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTcg                                                                                                                                 >  1:4607882/1‑150 (MQ=255)
                    gTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGcgtc                                                                                                                             >  1:881778/1‑150 (MQ=255)
                    gTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGcgtc                                                                                                                             <  1:623539/150‑1 (MQ=255)
                       aCTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCgtcgtc                                                                                                                          >  1:3243281/1‑150 (MQ=255)
                        ctgctgGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTcc                                                                                                                         >  1:1366163/1‑150 (MQ=255)
                        ctgctgGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTcc                                                                                                                         >  1:4330208/1‑150 (MQ=255)
                         tgctgGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTccc                                                                                                                        <  1:1753149/150‑1 (MQ=255)
                            tgGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCcac                                                                                                                     >  1:150419/1‑150 (MQ=255)
                            tgGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCcac                                                                                                                     >  2:866034/1‑150 (MQ=255)
                            tgGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCcac                                                                                                                     >  1:3586554/1‑150 (MQ=255)
                               gTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGc                                                                                                                  <  1:2066270/150‑1 (MQ=255)
                                ttATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCg                                                                                                                 <  2:904728/150‑1 (MQ=255)
                                     tCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCAtt                                                                                                            <  1:4540419/150‑1 (MQ=255)
                                        ttattaATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTc                                                                                                         <  2:3454334/150‑1 (MQ=255)
                                         tattaATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCa                                                                                                        <  2:2512583/150‑1 (MQ=255)
                                           ttaATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAac                                                                                                      <  2:3949782/150‑1 (MQ=255)
                                              aTTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAacgac                                                                                                   <  1:2136966/150‑1 (MQ=255)
                                               tttCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAacgacg                                                                                                  <  2:1310224/150‑1 (MQ=255)
                                               tttCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAacgacg                                                                                                  <  2:5150777/150‑1 (MQ=255)
                                                  cATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTAc                                                                                               >  2:2119454/1‑150 (MQ=255)
                                                   aTTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCATCATTGTCAACGACGTAcc                                                                                              >  1:114690/1‑150 (MQ=255)
                                                         aaCTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGa                                                                                        >  1:3014373/1‑150 (MQ=255)
                                                          aCTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGAt                                                                                       <  1:1026367/150‑1 (MQ=255)
                                                          aCTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGAt                                                                                       <  1:378406/150‑1 (MQ=255)
                                                             ccTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCt                                                                                    >  1:2467007/1‑150 (MQ=255)
                                                               tGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCtta                                                                                  >  2:193500/1‑148 (MQ=255)
                                                                 aaaGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACa                                                                                <  2:725876/150‑1 (MQ=255)
                                                                  aaGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACaa                                                                               >  2:3876432/1‑150 (MQ=255)
                                                                   aGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAg                                                                              >  2:1234072/1‑150 (MQ=255)
                                                                   aGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAg                                                                              >  1:1436308/1‑150 (MQ=255)
                                                                    gCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAgg                                                                             >  1:1571538/1‑150 (MQ=255)
                                                                      gAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTa                                                                           >  2:4682455/1‑150 (MQ=255)
                                                                       aaCCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTAc                                                                          <  2:472524/150‑1 (MQ=255)
                                                                           ataataTTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGTTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaa                                                                      <  2:179152/150‑1 (MQ=255)
                                                                           ataataTTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaa                                                                      <  2:2836720/150‑1 (MQ=255)
                                                                           ataataTTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaa                                                                      <  1:3886271/150‑1 (MQ=255)
                                                                                       cATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCAt                                                          <  2:4202146/150‑1 (MQ=255)
                                                                                         tAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATAc                                                        >  1:3665713/1‑150 (MQ=255)
                                                                                          aaTTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACt                                                       <  2:3598997/150‑1 (MQ=255)
                                                                                           aTTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTc                                                      >  1:4071378/1‑150 (MQ=255)
                                                                                           aTTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTc                                                      >  1:1647385/1‑150 (MQ=255)
                                                                                           aTTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTc                                                      <  2:2385133/150‑1 (MQ=255)
                                                                                           aTTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTc                                                      >  1:3436512/1‑150 (MQ=255)
                                                                                            tttGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCg                                                     <  2:2263862/150‑1 (MQ=255)
                                                                                              tgtgACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAg                                                   >  2:1472262/1‑150 (MQ=255)
                                                                                               gtgACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAgg                                                  <  2:1867025/150‑1 (MQ=255)
                                                                                                tgACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGc                                                 >  1:2269048/1‑150 (MQ=255)
                                                                                                 gACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCa                                                >  1:4605806/1‑150 (MQ=255)
                                                                                                 gACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCa                                                >  1:82457/1‑150 (MQ=255)
                                                                                                   cGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCAtt                                              <  2:4170020/150‑1 (MQ=255)
                                                                                                    gATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTg                                             <  2:1556269/150‑1 (MQ=255)
                                                                                                    gATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTg                                             >  1:340423/1‑150 (MQ=255)
                                                                                                     aTGAATTAAACACGGGGTTTAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTgg                                            <  2:2971951/150‑1 (MQ=255)
                                                                                                      tGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGc                                           <  2:3697342/150‑1 (MQ=255)
                                                                                                       gAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCa                                          <  1:2917744/150‑1 (MQ=255)
                                                                                                           tAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAAtt                                      <  2:2211440/150‑1 (MQ=255)
                                                                                                           tAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAAtt                                      <  2:3585487/150‑1 (MQ=255)
                                                                                                            aaaCACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAAttt                                     <  1:4511274/150‑1 (MQ=255)
                                                                                                            aaaCACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAAttt                                     <  1:2217271/150‑1 (MQ=255)
                                                                                                                   gggTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCt                              >  1:1884302/1‑150 (MQ=255)
                                                                                                                    ggTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTg                             <  1:589448/150‑1 (MQ=255)
                                                                                                                      ttAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGc                           <  1:2025805/150‑1 (MQ=255)
                                                                                                                        aaaTTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCaa                         >  1:3330692/1‑150 (MQ=255)
                                                                                                                        aaaTTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCaa                         <  2:3060783/150‑1 (MQ=255)
                                                                                                                          aTTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGc                       >  1:2635683/1‑150 (MQ=255)
                                                                                                                          aTTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGc                       <  1:2498347/150‑1 (MQ=255)
                                                                                                                           ttGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGcc                      >  1:4179315/1‑150 (MQ=255)
                                                                                                                            tGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCt                     <  1:494562/150‑1 (MQ=255)
                                                                                                                            tGAGCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTAGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACACACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGGATTGGCAAATTTATTATCTGGCAATCCt                     <  2:3224213/150‑1 (MQ=255)
                                                                                                                             gATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTg                    <  1:4674709/150‑1 (MQ=255)
                                                                                                                              aTCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGa                   >  1:1740509/1‑150 (MQ=255)
                                                                                                                              aTCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGa                   >  2:1106883/1‑150 (MQ=255)
                                                                                                                               tCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGaa                  >  2:3482527/1‑150 (MQ=255)
                                                                                                                               tCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCATCGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATGGGCCAATTTATTATCTGGCAAGCCGGaa                  >  2:1664175/1‑150 (MQ=255)
                                                                                                                                cGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGaaa                 >  1:3168939/1‑150 (MQ=255)
                                                                                                                                  aTGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAAc               <  2:150419/150‑1 (MQ=255)
                                                                                                                                  aTGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAAc               >  1:599592/1‑150 (MQ=255)
                                                                                                                                    ggTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGa             <  1:4884138/150‑1 (MQ=255)
                                                                                                                                        gCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATc         <  2:3796553/150‑1 (MQ=255)
                                                                                                                                          tGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgt       <  2:4496196/150‑1 (MQ=255)
                                                                                                                                           gTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgtt      <  1:3115726/150‑1 (MQ=255)
                                                                                                                                            tCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgttg     <  2:4631102/150‑1 (MQ=255)
                                                                                                                                            tCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgttg     <  1:1965004/150‑1 (MQ=255)
                                                                                                                                            tCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgttg     <  2:4330208/150‑1 (MQ=255)
                                                                                                                                             cAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgttgt    <  2:3188606/150‑1 (MQ=255)
                                                                                                                                              aaCTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgttgtt   >  2:4397770/1‑150 (MQ=255)
                                                                                                                                              aaCTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgttgtt   <  1:2119204/150‑1 (MQ=255)
                                                                                                                                               aCTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTa  <  2:2594202/150‑1 (MQ=255)
                                                                                                                                               aCTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTa  >  1:708574/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTA  >  CP009273/2474207‑2474499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATCTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGG  >  CP009273/2474204‑2474502
                                                                                                                                                     |                                                                                                                                                     
AATCTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTT                                                                                                                                                       <  SRR19973063.853690/150‑1 (MQ=60)
AATCTCATTTGCAGAAATAGAAAGTGACTGCTGGGTGATTTCATTATTAATTTCATTAAGAACTCCTGAAAGAGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATAGATGGTTGCTGTAAACTT                                                                                                                                                       >  SRR19973063.3932283/1‑150 (MQ=60)
           CAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTA                                                                                                                                            >  SRR19973063.4004065/1‑150 (MQ=60)
           CAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTA                                                                                                                                            >  SRR19973063.3626495/1‑150 (MQ=60)
           CAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTA                                                                                                                                            >  SRR19973063.3626428/1‑150 (MQ=60)
            AGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAA                                                                                                                                           >  SRR19973063.2196212/1‑150 (MQ=60)
             GAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAAC                                                                                                                                          >  SRR19973063.2271073/1‑150 (MQ=60)
             GAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAAC                                                                                                                                          >  SRR19973063.3228334/1‑150 (MQ=60)
              AAATAGAAAGTGACTGCTGTGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACAATAATATATATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATTGTGTAACG                                                                                                                                         >  SRR19973063.5074289/1‑150 (MQ=60)
              AAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACG                                                                                                                                         >  SRR19973063.4091637/1‑150 (MQ=60)
               AATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGA                                                                                                                                        >  SRR19973063.4451905/1‑150 (MQ=60)
               AATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGA                                                                                                                                        >  SRR19973063.2422620/1‑150 (MQ=60)
               AATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACGATGGTGTAACGA                                                                                                                                        >  SRR19973063.3060113/1‑150 (MQ=60)
                ATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGAT                                                                                                                                       >  SRR19973063.3703485/1‑150 (MQ=60)
                ATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGAT                                                                                                                                       <  SRR19973063.131765/150‑1 (MQ=60)
                 TAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATT                                                                                                                                      <  SRR19973063.1689286/150‑1 (MQ=60)
                   GAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCG                                                                                                                                    >  SRR19973063.710294/1‑150 (MQ=60)
                   GAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCG                                                                                                                                    >  SRR19973063.559386/1‑150 (MQ=60)
                   GAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCG                                                                                                                                    >  SRR19973063.4622619/1‑150 (MQ=60)
                       gtgtcttcttgtgTATTTCATTATTATTTTCATTAAGAACTCCTGTAAGCGAACCATTATATTTCTCGATAATTTGTGACTTTTATTTATTCTCGGGGTTAAATTTATCGATGTTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTC                                                                                                                                <  SRR19973063.3941143/137‑1 (MQ=60)
                       GTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTC                                                                                                                                >  SRR19973063.884135/1‑150 (MQ=60)
                       GTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTC                                                                                                                                >  SRR19973063.884325/1‑150 (MQ=60)
                       GTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTC                                                                                                                                <  SRR19973063.625372/150‑1 (MQ=60)
                          ACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTC                                                                                                                             >  SRR19973063.3253698/1‑150 (MQ=60)
                           CTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCC                                                                                                                            >  SRR19973063.1370279/1‑150 (MQ=60)
                           CTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCC                                                                                                                            >  SRR19973063.4344006/1‑150 (MQ=60)
                            TGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCC                                                                                                                           <  SRR19973063.1758384/150‑1 (MQ=60)
                            TGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCC                                                                                                                           >  SRR19973063.2969421/1‑150 (MQ=60)
                            TGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCC                                                                                                                           <  SRR19973063.1758531/150‑1 (MQ=60)
                            TGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCC                                                                                                                           >  SRR19973063.1743832/1‑150 (MQ=60)
                              CTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCA                                                                                                                         >  SRR19973063.1946338/1‑150 (MQ=60)
                               TGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGTTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCAC                                                                                                                        >  SRR19973063.150883/1‑150 (MQ=60)
                               TGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCAC                                                                                                                        >  SRR19973063.3882640/1‑150 (MQ=60)
                               TGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCAC                                                                                                                        >  SRR19973063.150803/1‑150 (MQ=60)
                               TGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCAC                                                                                                                        >  SRR19973063.3598052/1‑150 (MQ=60)
                                  GTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGC                                                                                                                     <  SRR19973063.2072729/150‑1 (MQ=60)
                                  GTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGC                                                                                                                     <  SRR19973063.3281418/150‑1 (MQ=60)
                                        TCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATT                                                                                                               <  SRR19973063.1976217/150‑1 (MQ=60)
                                        TCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATT                                                                                                               <  SRR19973063.4554973/150‑1 (MQ=60)
                                             ATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAA                                                                                                          <  SRR19973063.2329725/150‑1 (MQ=60)
                                             ATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAA                                                                                                          <  SRR19973063.2329649/150‑1 (MQ=60)
                                              TTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAAC                                                                                                         <  SRR19973063.3506846/150‑1 (MQ=60)
                                              TTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAAC                                                                                                         <  SRR19973063.4635049/150‑1 (MQ=60)
                                               TAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACG                                                                                                        >  SRR19973063.3495970/1‑150 (MQ=60)
                                                 ATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGAC                                                                                                      <  SRR19973063.2143638/150‑1 (MQ=60)
                                                    TCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTA                                                                                                   <  SRR19973063.4546967/150‑1 (MQ=60)
                                                      ATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACC                                                                                                 >  SRR19973063.1801369/1‑150 (MQ=60)
                                                      ATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCATCATTGTCAACGACGTACC                                                                                                 >  SRR19973063.115074/1‑150 (MQ=60)
                                                       TTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCA                                                                                                <  SRR19973063.2476144/150‑1 (MQ=60)
                                                       TTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCA                                                                                                <  SRR19973063.2476147/150‑1 (MQ=60)
                                                           GAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTG                                                                                            >  SRR19973063.1329103/1‑150 (MQ=60)
                                                            AACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGA                                                                                           <  SRR19973063.1249947/150‑1 (MQ=60)
                                                            AACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGA                                                                                           >  SRR19973063.3023990/1‑150 (MQ=60)
                                                             ACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGAT                                                                                          <  SRR19973063.1029462/150‑1 (MQ=60)
                                                             ACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGAT                                                                                          <  SRR19973063.379486/150‑1 (MQ=60)
                                                                CCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCT                                                                                       >  SRR19973063.4468954/1‑150 (MQ=60)
                                                                CCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCT                                                                                       >  SRR19973063.5107845/1‑150 (MQ=60)
                                                                CCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCT                                                                                       >  SRR19973063.2474891/1‑150 (MQ=60)
                                                                  TGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTA                                                                                     <  SRR19973063.611416/150‑1 (MQ=60)
                                                                    AAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACA                                                                                   <  SRR19973063.2442043/150‑1 (MQ=60)
                                                                    AAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACA                                                                                   >  SRR19973063.4576823/1‑150 (MQ=60)
                                                                    AAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACA                                                                                   <  SRR19973063.4037488/150‑1 (MQ=60)
                                                                      AGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAG                                                                                 >  SRR19973063.1440691/1‑150 (MQ=60)
                                                                      AGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAG                                                                                 >  SRR19973063.1290458/1‑150 (MQ=60)
                                                                       GCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGG                                                                                >  SRR19973063.1576365/1‑150 (MQ=60)
                                                                       GCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGG                                                                                >  SRR19973063.1576363/1‑150 (MQ=60)
                                                                        CGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGT                                                                               >  SRR19973063.4300823/1‑150 (MQ=60)
                                                                              ATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAA                                                                         <  SRR19973063.3898615/150‑1 (MQ=60)
                                                                              ATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAA                                                                         <  SRR19973063.3898484/150‑1 (MQ=60)
                                                                                     TTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTA                                                                  <  SRR19973063.5067718/150‑1 (MQ=60)
                                                                                            TAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATAC                                                           <  SRR19973063.3410015/150‑1 (MQ=60)
                                                                                            TAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATAC                                                           >  SRR19973063.3677460/1‑150 (MQ=60)
                                                                                             AATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACT                                                          <  SRR19973063.4597255/150‑1 (MQ=60)
                                                                                              ATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTC                                                         >  SRR19973063.3213663/1‑150 (MQ=60)
                                                                                              ATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTC                                                         >  SRR19973063.4084426/1‑150 (MQ=60)
                                                                                              ATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTC                                                         >  SRR19973063.3447442/1‑150 (MQ=60)
                                                                                              ATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTC                                                         >  SRR19973063.1770938/1‑150 (MQ=60)
                                                                                              ATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTC                                                         >  SRR19973063.1652417/1‑150 (MQ=60)
                                                                                                   TGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGC                                                    <  SRR19973063.4393579/150‑1 (MQ=60)
                                                                                                   TGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGC                                                    >  SRR19973063.2276288/1‑150 (MQ=60)
                                                                                                    GACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCA                                                   >  SRR19973063.4620537/1‑150 (MQ=60)
                                                                                                    GACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCA                                                   >  SRR19973063.82742/1‑150 (MQ=60)
                                                                                                      CGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATT                                                 >  SRR19973063.2815764/1‑150 (MQ=60)
                                                                                                      CGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATT                                                 <  SRR19973063.1515074/150‑1 (MQ=60)
                                                                                                       GATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTG                                                >  SRR19973063.341398/1‑150 (MQ=60)
                                                                                                       GATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTG                                                >  SRR19973063.341404/1‑150 (MQ=60)
                                                                                                         TGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGC                                              <  SRR19973063.3667761/150‑1 (MQ=60)
                                                                                                         TGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGC                                              <  SRR19973063.3669639/150‑1 (MQ=60)
                                                                                                          GAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCA                                             <  SRR19973063.2927049/150‑1 (MQ=60)
                                                                                                           AATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAA                                            <  SRR19973063.870310/150‑1 (MQ=60)
                                                                                                               AAACACGGGGTTATATTGATCGATTGTTGCTGTCAACTTTCTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTAATTTTAAACGACGTACCATCTGATACTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTT                                        <  SRR19973063.1523242/150‑1 (MQ=60)
                                                                                                               AAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTT                                        <  SRR19973063.2224266/150‑1 (MQ=60)
                                                                                                               AAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTT                                        <  SRR19973063.4525756/150‑1 (MQ=60)
                                                                                                               AAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTT                                        <  SRR19973063.2096278/150‑1 (MQ=60)
                                                                                                                AACACGGGGTTAAATTGATCGATGTTAGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATAGTCAACGACGAACCAACTTATCCTTACAAGGTACGAAAAAAATTACTAATAATCGAGGCATAGTCAAATTTA                                       >  SRR19973063.3416608/1‑150 (MQ=60)
                                                                                                                    CGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTAT                                   >  SRR19973063.1402919/1‑150 (MQ=60)
                                                                                                                     GGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATC                                  >  SRR19973063.4080514/1‑150 (MQ=60)
                                                                                                                      GGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCT                                 >  SRR19973063.1890192/1‑150 (MQ=60)
                                                                                                                      GGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCT                                 >  SRR19973063.4469480/1‑150 (MQ=60)
                                                                                                                       GGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTG                                <  SRR19973063.591187/150‑1 (MQ=60)
                                                                                                                       GGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTG                                <  SRR19973063.591410/150‑1 (MQ=60)
                                                                                                                       GGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTG                                <  SRR19973063.2574464/150‑1 (MQ=60)
                                                                                                                         TTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGC                              <  SRR19973063.2032186/150‑1 (MQ=60)
                                                                                                                         TTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGC                              <  SRR19973063.4620911/150‑1 (MQ=60)
                                                                                                                         TTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGC                              <  SRR19973063.2032129/150‑1 (MQ=60)
                                                                                                                          TAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCA                             >  SRR19973063.422578/1‑150 (MQ=60)
                                                                                                                          TAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCA                             >  SRR19973063.2142955/1‑150 (MQ=60)
                                                                                                                          TAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCA                             >  SRR19973063.2120863/1‑150 (MQ=60)
                                                                                                                           AAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAA                            >  SRR19973063.3341321/1‑150 (MQ=60)
                                                                                                                            AATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAG                           >  SRR19973063.2798365/1‑150 (MQ=60)
                                                                                                                             ATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGC                          <  SRR19973063.2506315/150‑1 (MQ=60)
                                                                                                                             ATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGC                          >  SRR19973063.2644095/1‑150 (MQ=60)
                                                                                                                              TTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCC                         >  SRR19973063.4192665/1‑150 (MQ=60)
                                                                                                                               TGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCT                        <  SRR19973063.495841/150‑1 (MQ=60)
                                                                                                                               TGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCT                        <  SRR19973063.495851/150‑1 (MQ=60)
                                                                                                                               TGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCT                        <  SRR19973063.495983/150‑1 (MQ=60)
                                                                                                                                GATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTG                       <  SRR19973063.4689620/150‑1 (MQ=60)
                                                                                                                                 ATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGA                      >  SRR19973063.1745804/1‑150 (MQ=60)
                                                                                                                                  TCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAA                     >  SRR19973063.2010856/1‑150 (MQ=60)
                                                                                                                                   CGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAA                    >  SRR19973063.3978384/1‑150 (MQ=60)
                                                                                                                                   CGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAA                    >  SRR19973063.3179139/1‑150 (MQ=60)
                                                                                                                                    GATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAA                   <  SRR19973063.3153388/150‑1 (MQ=60)
                                                                                                                                     ATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAAC                  >  SRR19973063.601390/1‑150 (MQ=60)
                                                                                                                                     ATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAAC                  >  SRR19973063.601356/1‑150 (MQ=60)
                                                                                                                                     ATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAAC                  <  SRR19973063.81830/150‑1 (MQ=60)
                                                                                                                                      TGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACG                 >  SRR19973063.3089943/1‑150 (MQ=60)
                                                                                                                                       GGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGA                <  SRR19973063.4899891/150‑1 (MQ=60)
                                                                                                                                        GTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAA               >  SRR19973063.4628785/1‑150 (MQ=60)
                                                                                                                                        GTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAA               >  SRR19973063.4805789/1‑150 (MQ=60)
                                                                                                                                              GTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTT         <  SRR19973063.3125755/150‑1 (MQ=60)
                                                                                                                                              GTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTT         >  SRR19973063.1431290/1‑150 (MQ=60)
                                                                                                                                               TCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTG        <  SRR19973063.1971153/150‑1 (MQ=60)
                                                                                                                                               TCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTG        <  SRR19973063.2205506/150‑1 (MQ=60)
                                                                                                                                                 AACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTT      <  SRR19973063.2125832/150‑1 (MQ=60)
                                                                                                                                                 AACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTT      >  SRR19973063.1030252/1‑150 (MQ=60)
                                                                                                                                                  ACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTA     >  SRR19973063.710593/1‑150 (MQ=60)
                                                                                                                                                  ACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTA     >  SRR19973063.710657/1‑150 (MQ=60)
                                                                                                                                                     TACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGG  >  SRR19973063.3934180/1‑150 (MQ=60)
                                                                                                                                                     TACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGG  >  SRR19973063.3934162/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AATCTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGG  >  CP009273/2474204‑2474502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑