Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F342 I0 R1	1331	225.9	7641038	95.8%	7320114	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,474,353	G→T	100%	Q435K (CAG→AAG)	emrY ←	putative multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,474,353	0	G	T	99.2%	798.0 / ‑2.0	248	Q435K (CAG→AAG)	emrY	putative multidrug efflux system
Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (129/116); total (131/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 249 total aligned reads displayed.

TCTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGT  >  CP009273/2474206‑2474497

   cATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATCATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACt                                                                                                                                               <  1:3725082/148‑1 (MQ=255)

   cATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACt                                                                                                                                               <  2:1239131/148‑1 (MQ=255)

     tttGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTAt                                                                                                                                             >  2:2494018/1‑148 (MQ=255)

     tttGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTAt                                                                                                                                             <  1:2390912/148‑1 (MQ=255)

     tttGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTAt                                                                                                                                             >  2:1874206/1‑148 (MQ=255)

     tttGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTAt                                                                                                                                             >  2:3685843/1‑148 (MQ=255)

     tttGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTAt                                                                                                                                             <  1:1942032/148‑1 (MQ=255)

      ttGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATg                                                                                                                                            >  1:423375/1‑148 (MQ=255)

           gAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTa                                                                                                                                       <  2:2336907/148‑1 (MQ=255)

           gAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTa                                                                                                                                       >  2:2463801/1‑148 (MQ=255)

            aaaTAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTaa                                                                                                                                      >  2:1794148/1‑148 (MQ=255)

            aaaTAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTaa                                                                                                                                      >  2:395574/1‑148 (MQ=255)

             aaTAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAAc                                                                                                                                     >  2:670396/1‑148 (MQ=255)

              aTAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACg                                                                                                                                    >  2:79704/1‑148 (MQ=255)

              aTAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACg                                                                                                                                    <  1:797419/148‑1 (MQ=255)

                aGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGAt                                                                                                                                  >  1:627728/1‑148 (MQ=255)

                 gAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGAtt                                                                                                                                 <  1:784768/148‑1 (MQ=255)

                  aaaGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTc                                                                                                                                >  2:874763/1‑148 (MQ=255)

                       gACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGcgtc                                                                                                                           <  2:202388/148‑1 (MQ=255)

                           gctgGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTcc                                                                                                                       >  2:2735871/1‑148 (MQ=255)

                              gggTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCca                                                                                                                    <  2:2964518/148‑1 (MQ=255)

                               ggTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATAAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTCACTATGGTGTAACGATTCGCGTCGTCCCcac                                                                                                                   >  1:3493464/1‑148 (MQ=255)

                                 ttATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAg                                                                                                                 >  1:189211/1‑148 (MQ=255)

                                 ttATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAg                                                                                                                 >  1:490356/1‑148 (MQ=255)

                                 ttATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAg                                                                                                                 >  1:67713/1‑148 (MQ=255)

                                   aTTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCg                                                                                                               <  2:2233918/148‑1 (MQ=255)

                                   aTTTAATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTTCTGTCAACTTACTATGGTGTAACGATTCTCGTCTTCCCCACAGCg                                                                                                               >  2:2193263/1‑148 (MQ=255)

                                     ttCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTc                                                                                                             >  2:982420/1‑148 (MQ=255)

                                      tCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCa                                                                                                            <  1:309184/148‑1 (MQ=255)

                                             taATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCaa                                                                                                     >  2:284222/1‑148 (MQ=255)

                                             taATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCaa                                                                                                     >  1:3553235/1‑148 (MQ=255)

                                             taATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCaa                                                                                                     >  1:572047/1‑148 (MQ=255)

                                                tttCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAacga                                                                                                  >  2:106590/1‑148 (MQ=255)

                                                 ttCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAacgac                                                                                                 >  2:929576/1‑148 (MQ=255)

                                                  tCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAacgacg                                                                                                <  1:203604/148‑1 (MQ=255)

                                                    aTTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTa                                                                                              <  1:1765165/148‑1 (MQ=255)

                                                      tAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTAcc                                                                                            >  2:1276988/1‑148 (MQ=255)

                                                       aaGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCa                                                                                           <  2:2752625/148‑1 (MQ=255)

                                                        aGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCaa                                                                                          <  2:3555970/148‑1 (MQ=255)

                                                        aGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCaa                                                                                          <  2:1650402/148‑1 (MQ=255)

                                                           aCTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTg                                                                                       >  2:718886/1‑148 (MQ=255)

                                                           aCTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTg                                                                                       >  2:585778/1‑148 (MQ=255)

                                                            cTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGa                                                                                      <  1:3813962/148‑1 (MQ=255)

                                                             tCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGAt                                                                                     >  2:1482677/1‑148 (MQ=255)

                                                              ccTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATc                                                                                    <  2:319480/148‑1 (MQ=255)

                                                               cTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATcc                                                                                   <  2:556915/148‑1 (MQ=255)

                                                               cTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATcc                                                                                   <  1:2999497/148‑1 (MQ=255)

                                                                 gAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCtt                                                                                 >  1:1885357/1‑147 (MQ=255)

                                                                 gAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCtt                                                                                 >  1:1415969/1‑147 (MQ=255)

                                                                  aaaGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCtta                                                                                <  1:1102023/148‑3 (MQ=255)

                                                                    aGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACa                                                                              >  1:2312492/1‑148 (MQ=255)

                                                                    aGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACa                                                                              >  1:2774055/1‑148 (MQ=255)

                                                                      cGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAg                                                                            <  1:3243326/148‑1 (MQ=255)

                                                                       gAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAgg                                                                           <  1:3057652/148‑1 (MQ=255)

                                                                         aCCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTa                                                                         >  1:1372021/1‑148 (MQ=255)

                                                                           cATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACg                                                                       >  1:3308932/1‑148 (MQ=255)

                                                                            ataataTTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGa                                                                      >  1:1608418/1‑148 (MQ=255)

                                                                               atatTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaaa                                                                   >  1:3366944/1‑148 (MQ=255)

                                                                               atatTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaaa                                                                   >  1:1465217/1‑148 (MQ=255)

                                                                                 atTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaaaaa                                                                 <  2:490356/148‑1 (MQ=255)

                                                                                  tttATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaaaaaa                                                                >  2:2596809/1‑148 (MQ=255)

                                                                                    tATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAAtt                                                              <  1:2200035/148‑1 (MQ=255)

                                                                                     aTCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTa                                                             >  2:1748236/1‑148 (MQ=255)

                                                                                        cATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTc                                                          <  1:1696134/148‑1 (MQ=255)

                                                                                         aTAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCa                                                         <  1:3196167/148‑1 (MQ=255)

                                                                                             tttGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACt                                                     >  2:623743/1‑148 (MQ=255)

                                                                                                    cGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCa                                              <  1:2071849/148‑1 (MQ=255)

                                                                                                      aTGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCAtt                                            >  2:3515473/1‑148 (MQ=255)

                                                                                                       tGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTg                                           >  1:3535158/1‑148 (MQ=255)

                                                                                                         aaTTAAACACGTGGTTATATTGATCGATGGTTGCTGTCAACTTACTATGGTTTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCATCTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGc                                         <  2:3619100/148‑1 (MQ=255)

                                                                                                          aTTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCa                                        >  2:838905/1‑148 (MQ=255)

                                                                                                           ttAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCaa                                       <  1:2324809/148‑1 (MQ=255)

                                                                                                            tAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCaaa                                      >  1:1165874/1‑148 (MQ=255)

                                                                                                             aaaCACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAAt                                     <  1:2513021/148‑1 (MQ=255)

                                                                                                               acacGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAAttt                                   >  2:1680155/1‑148 (MQ=255)

                                                                                                                 acGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATttat                                 <  2:3493555/148‑1 (MQ=255)

                                                                                                                 acGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATttat                                 <  2:1025951/148‑1 (MQ=255)

                                                                                                                  cGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATttatt                                <  1:1278319/148‑1 (MQ=255)

                                                                                                                  cGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATttatt                                <  1:2992467/148‑1 (MQ=255)

                                                                                                                   ggggTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATttatta                               <  2:924691/148‑1 (MQ=255)

                                                                                                                   ggggTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATttatta                               <  2:2820289/148‑1 (MQ=255)

                                                                                                                   ggggTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATttatta                               <  2:2360765/148‑1 (MQ=255)

                                                                                                                    gggTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTtattat                              >  1:1009503/1‑148 (MQ=255)

                                                                                                                    gggTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTtattat                              <  1:948273/148‑1 (MQ=255)

                                                                                                                    gggTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTtattat                              <  2:1917337/148‑1 (MQ=255)

                                                                                                                       ttAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTg                           >  2:850916/1‑148 (MQ=255)

                                                                                                                             tGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGc                     >  1:521579/1‑148 (MQ=255)

                                                                                                                             tGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGc                     <  2:3108819/148‑1 (MQ=255)

                                                                                                                              gATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGcc                    <  2:3220436/148‑1 (MQ=255)

                                                                                                                               aTCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCt                   >  1:225887/1‑148 (MQ=255)

                                                                                                                                tCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTg                  <  1:1497961/148‑1 (MQ=255)

                                                                                                                                       ttGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGa           >  1:1945263/1‑148 (MQ=255)

                                                                                                                                        tGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGaa          >  1:3328362/1‑148 (MQ=255)

                                                                                                                                         gCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGaaa         <  2:1971899/148‑1 (MQ=255)

                                                                                                                                         gCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGaaa         <  1:718886/148‑1 (MQ=255)

                                                                                                                                            gTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCg      <  2:1472620/148‑1 (MQ=255)

                                                                                                                                               aaCTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgttg   <  1:2494018/148‑1 (MQ=255)

                                                                                                                                               aaCTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgttg   <  1:3685843/148‑1 (MQ=255)

                                                                                                                                                aCTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACCAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAAGCgtggt  >  2:3722199/1‑148 (MQ=255)

                                                                                                                                                aCTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgttgt  <  2:3315135/148‑1 (MQ=255)

TCTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGT  >  CP009273/2474206‑2474497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AATCTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAG  >  CP009273/2474204‑2474501

AATCTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTG                                                                                                                                                      >  SRR19973069.3753390/1‑150 (MQ=60)

AATCTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTG                                                                                                                                                      <  SRR19973069.1871521/150‑1 (MQ=60)

AATCTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTG                                                                                                                                                      <  SRR19973069.1871545/150‑1 (MQ=60)

     CATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATCATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTAT                                                                                                                                                 <  SRR19973069.3749389/150‑1 (MQ=60)

      ATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATG                                                                                                                                                >  SRR19973069.426481/1‑150 (MQ=60)

       TTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGG                                                                                                                                               <  SRR19973069.2407116/150‑1 (MQ=60)

       TTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGG                                                                                                                                               <  SRR19973069.1270472/150‑1 (MQ=60)

       TTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGG                                                                                                                                               <  SRR19973069.1955966/150‑1 (MQ=60)

       TTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGG                                                                                                                                               <  SRR19973069.1397339/150‑1 (MQ=60)

       TTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGG                                                                                                                                               <  SRR19973069.1955406/150‑1 (MQ=60)

               AATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGA                                                                                                                                       >  SRR19973069.1667719/1‑150 (MQ=60)

               AATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGA                                                                                                                                       >  SRR19973069.3574514/1‑150 (MQ=60)

                ATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGAT                                                                                                                                      >  SRR19973069.631892/1‑150 (MQ=60)

                ATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGAT                                                                                                                                      >  SRR19973069.631888/1‑150 (MQ=60)

                ATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGAT                                                                                                                                      >  SRR19973069.24102/1‑150 (MQ=60)

                ATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGAT                                                                                                                                      <  SRR19973069.803073/150‑1 (MQ=60)

                ATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGAT                                                                                                                                      <  SRR19973069.3605713/150‑1 (MQ=60)

                   GAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCG                                                                                                                                   <  SRR19973069.3245420/150‑1 (MQ=60)

                   GAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCG                                                                                                                                   <  SRR19973069.790335/150‑1 (MQ=60)

                     AAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCG                                                                                                                                 >  SRR19973069.1370700/1‑150 (MQ=60)

                     AAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCG                                                                                                                                 >  SRR19973069.2054816/1‑150 (MQ=60)

                          ACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTC                                                                                                                            <  SRR19973069.1910922/150‑1 (MQ=60)

                          ACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTC                                                                                                                            <  SRR19973069.1910952/150‑1 (MQ=60)

                              cggcGTTATTTCATTATTACTTTCATTAAGAACGCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACCCGGGGTTAACTTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCA                                                                                                                        <  SRR19973069.1983410/146‑1 (MQ=60)

                              CTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCTCA                                                                                                                        <  SRR19973069.2480341/150‑1 (MQ=60)

                              CTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCA                                                                                                                        >  SRR19973069.2098211/1‑150 (MQ=60)

                              CTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCA                                                                                                                        >  SRR19973069.3533914/1‑150 (MQ=60)

                              CTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCA                                                                                                                        >  SRR19973069.3037775/1‑150 (MQ=60)

                               TGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTTATAGATGGATGCTGTCAAATTACTATGGTGTAAAGATTCGCGTCGTCCACAC                                                                                                                       >  SRR19973069.3075126/1‑150 (MQ=60)

                               TGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCAC                                                                                                                       >  SRR19973069.1482879/1‑150 (MQ=60)

                               TGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATAAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTCACTATGGTGTAACGATTCGCGTCGTCCCCAC                                                                                                                       >  SRR19973069.3515979/1‑150 (MQ=60)

                                GGGTTATTTCAATATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACA                                                                                                                      <  SRR19973069.3624417/150‑1 (MQ=60)

                                 GGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAG                                                                                                                     >  SRR19973069.190764/1‑150 (MQ=60)

                                 GGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAG                                                                                                                     >  SRR19973069.493890/1‑150 (MQ=60)

                                 GGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAG                                                                                                                     >  SRR19973069.68492/1‑150 (MQ=60)

                                        TCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATT                                                                                                              <  SRR19973069.311258/150‑1 (MQ=60)

                                         CATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTG                                                                                                             <  SRR19973069.2521632/150‑1 (MQ=60)

                                          ATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGT                                                                                                            >  SRR19973069.190220/1‑150 (MQ=60)

                                           TTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTC                                                                                                           >  SRR19973069.345035/1‑150 (MQ=60)

                                             ATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAA                                                                                                         >  SRR19973069.542816/1‑150 (MQ=60)

                                             ATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAA                                                                                                         >  SRR19973069.3576332/1‑150 (MQ=60)

                                             ATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAA                                                                                                         >  SRR19973069.576004/1‑150 (MQ=60)

                                              TTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAAC                                                                                                        <  SRR19973069.2600521/150‑1 (MQ=60)

                                              TTAATTTCATTAAGAACTCCTGAAAGCGAAACATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGTGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAAC                                                                                                        <  SRR19973069.2816056/150‑1 (MQ=60)

                                               TAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACG                                                                                                       >  SRR19973069.776379/1‑150 (MQ=60)

                                                AATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGA                                                                                                      >  SRR19973069.2883801/1‑150 (MQ=60)

                                                    TCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTA                                                                                                  <  SRR19973069.205232/150‑1 (MQ=60)

                                                     CATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTAC                                                                                                 <  SRR19973069.1241924/150‑1 (MQ=60)

                                                     CATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTAC                                                                                                 >  SRR19973069.568763/1‑150 (MQ=60)

                                                      ATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATACTTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACC                                                                                                <  SRR19973069.3275074/150‑1 (MQ=60)

                                                      ATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACC                                                                                                >  SRR19973069.3192441/1‑150 (MQ=60)

                                                      ATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACC                                                                                                <  SRR19973069.1777610/150‑1 (MQ=60)

                                                             ACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGAT                                                                                         >  SRR19973069.807454/1‑150 (MQ=60)

                                                              CTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATC                                                                                        <  SRR19973069.3838650/150‑1 (MQ=60)

                                                                 CTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTT                                                                                     <  SRR19973069.3019402/150‑1 (MQ=60)

                                                                 CTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTT                                                                                     >  SRR19973069.1425845/1‑150 (MQ=60)

                                                                 CTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTT                                                                                     >  SRR19973069.1898444/1‑150 (MQ=60)

                                                                    AAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACA                                                                                  <  SRR19973069.2278900/150‑1 (MQ=60)

                                                                    AAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACA                                                                                  >  SRR19973069.2792533/1‑150 (MQ=60)

                                                                    AAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACA                                                                                  <  SRR19973069.1109724/150‑1 (MQ=60)

                                                                    AAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACA                                                                                  >  SRR19973069.2328226/1‑150 (MQ=60)

                                                                      AGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAG                                                                                >  SRR19973069.435064/1‑150 (MQ=60)

                                                                      AGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAG                                                                                >  SRR19973069.3516070/1‑150 (MQ=60)

                                                                        CGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGT                                                                              <  SRR19973069.3264552/150‑1 (MQ=60)

                                                                         GAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTA                                                                             >  SRR19973069.3295561/1‑150 (MQ=60)

                                                                         GAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTA                                                                             <  SRR19973069.3077883/150‑1 (MQ=60)

                                                                         GAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTA                                                                             <  SRR19973069.1160335/150‑1 (MQ=60)

                                                                         GAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTA                                                                             >  SRR19973069.1381576/1‑150 (MQ=60)

                                                                         GAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTA                                                                             >  SRR19973069.3720951/1‑150 (MQ=60)

                                                                         GAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTA                                                                             >  SRR19973069.1382345/1‑150 (MQ=60)

                                                                         GAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTA                                                                             >  SRR19973069.1884090/1‑150 (MQ=60)

                                                                          AACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTAC                                                                            >  SRR19973069.3365202/1‑150 (MQ=60)

                                                                           ACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG                                                                           >  SRR19973069.3330609/1‑150 (MQ=60)

                                                                           ACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG                                                                           >  SRR19973069.2235882/1‑150 (MQ=60)

                                                                            CCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGA                                                                          >  SRR19973069.1619233/1‑150 (MQ=60)

                                                                               TAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAA                                                                       >  SRR19973069.3388960/1‑150 (MQ=60)

                                                                               TAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAA                                                                       >  SRR19973069.1475438/1‑150 (MQ=60)

                                                                               TAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAA                                                                       >  SRR19973069.2512440/1‑150 (MQ=60)

                                                                                AATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAA                                                                      >  SRR19973069.583464/1‑150 (MQ=60)

                                                                                AATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAA                                                                      >  SRR19973069.1043288/1‑150 (MQ=60)

                                                                                      TATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTAC                                                                <  SRR19973069.880844/150‑1 (MQ=60)

                                                                                      TATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTAC                                                                <  SRR19973069.2214969/150‑1 (MQ=60)

                                                                                       ATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACT                                                               >  SRR19973069.310073/1‑150 (MQ=60)

                                                                                        TCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTC                                                              <  SRR19973069.3331353/150‑1 (MQ=60)

                                                                                          CATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCAT                                                            <  SRR19973069.1707989/150‑1 (MQ=60)

                                                                                           ATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATA                                                           <  SRR19973069.3216874/150‑1 (MQ=60)

                                                                                           ATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATA                                                           <  SRR19973069.3216923/150‑1 (MQ=60)

                                                                                             AATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACT                                                         <  SRR19973069.680413/150‑1 (MQ=60)

                                                                                                      CGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATT                                                <  SRR19973069.2086202/150‑1 (MQ=60)

                                                                                                      CGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATT                                                <  SRR19973069.143491/150‑1 (MQ=60)

                                                                                                      CGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATT                                                >  SRR19973069.2088940/1‑150 (MQ=60)

                                                                                                      CGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATT                                                <  SRR19973069.2399019/150‑1 (MQ=60)

                                                                                                       GATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTG                                               >  SRR19973069.3558068/1‑150 (MQ=60)

                                                                                                       GATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTG                                               <  SRR19973069.712632/150‑1 (MQ=60)

                                                                                                       GATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTG                                               >  SRR19973069.409649/1‑150 (MQ=60)

                                                                                                           AATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTagatcggaagagcacacgtc                                           >  SRR19973069.1726570/1‑130 (MQ=60)

                                                                                                            ATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAA                                          >  SRR19973069.1173975/1‑150 (MQ=60)

                                                                                                             TTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAAT                                         <  SRR19973069.2340618/150‑1 (MQ=60)

                                                                                                               AAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTT                                       <  SRR19973069.2529757/150‑1 (MQ=60)

                                                                                                                   ACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTA                                   <  SRR19973069.80572/150‑1 (MQ=60)

                                                                                                                    CGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTAT                                  <  SRR19973069.1286890/150‑1 (MQ=60)

                                                                                                                    CGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTAT                                  <  SRR19973069.3012988/150‑1 (MQ=60)

                                                                                                                    CGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTAT                                  >  SRR19973069.1016455/1‑150 (MQ=60)

                                                                                                                    CGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTAT                                  <  SRR19973069.3012338/150‑1 (MQ=60)

                                                                                                                      GGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCT                                <  SRR19973069.954656/150‑1 (MQ=60)

                                                                                                                      GGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCT                                <  SRR19973069.14982/150‑1 (MQ=60)

                                                                                                                      GGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCT                                <  SRR19973069.2338378/150‑1 (MQ=60)

                                                                                                                            AATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAG                          >  SRR19973069.778099/1‑150 (MQ=60)

                                                                                                                             ATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGC                         >  SRR19973069.523815/1‑150 (MQ=60)

                                                                                                                             ATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGC                         >  SRR19973069.525278/1‑150 (MQ=60)

                                                                                                                               TGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCT                       >  SRR19973069.227630/1‑150 (MQ=60)

                                                                                                                               TGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCT                       <  SRR19973069.2308932/150‑1 (MQ=60)

                                                                                                                                  TCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAA                    <  SRR19973069.1508344/150‑1 (MQ=60)

                                                                                                                                       GGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGA               >  SRR19973069.1958682/1‑150 (MQ=60)

                                                                                                                                       GGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGCTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGA               >  SRR19973069.1959175/1‑150 (MQ=60)

                                                                                                                                        GTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAA              >  SRR19973069.1632139/1‑150 (MQ=60)

                                                                                                                                        GTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAA              >  SRR19973069.3350734/1‑150 (MQ=60)

                                                                                                                                        GTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAA              >  SRR19973069.3350174/1‑150 (MQ=60)

                                                                                                                                           GCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATC           <  SRR19973069.723951/150‑1 (MQ=60)

                                                                                                                                             TGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGT         >  SRR19973069.1600690/1‑150 (MQ=60)

                                                                                                                                              GTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTT        >  SRR19973069.442361/1‑150 (MQ=60)

                                                                                                                                              GTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTT        >  SRR19973069.3827854/1‑150 (MQ=60)

                                                                                                                                               TCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTG       <  SRR19973069.1888473/150‑1 (MQ=60)

                                                                                                                                                 AACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTT     >  SRR19973069.2439877/1‑150 (MQ=60)

                                                                                                                                                 AACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTT     <  SRR19973069.3709950/150‑1 (MQ=60)

                                                                                                                                                 AACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTT     <  SRR19973069.2510693/150‑1 (MQ=60)

                                                                                                                                                  ACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTA    >  SRR19973069.979163/1‑150 (MQ=60)

                                                                                                                                                    TTACTATGGTGTATCGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAG  <  SRR19973069.286188/150‑1 (MQ=60)

AATCTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAG  >  CP009273/2474204‑2474501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`