Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,474,353 G→T 100% Q435K (CAG→AAG)  emrY ← putative multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,474,3530GT98.6% 665.2 / 0.5 208Q435K (CAG→AAG) emrYputative multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base G (3/0);  new base T (106/98);  total (110/98)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 208 total aligned reads displayed.

ATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTT  >  CP009273/2474210‑2474498
                                                                                                                                               |                                                                                                                                                 
  ttGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATg                                                                                                                                             >  2:1239340/1‑148 (MQ=255)
    gCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGgt                                                                                                                                           <  1:170062/148‑1 (MQ=255)
       gAAATAGAAAGTGACTGCTTGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTa                                                                                                                                        <  2:2641039/148‑1 (MQ=255)
        aaaTAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTaa                                                                                                                                       >  1:1055854/1‑148 (MQ=255)
        aaaTAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTaa                                                                                                                                       >  1:1056450/1‑148 (MQ=255)
           tAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGa                                                                                                                                    >  1:1039791/1‑148 (MQ=255)
            aGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGAt                                                                                                                                   <  1:1858715/148‑1 (MQ=255)
              aaaGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCTACTTACTATGGTGTAACGATTc                                                                                                                                 >  1:2941021/1‑148 (MQ=255)
              aaaGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTc                                                                                                                                 >  2:872482/1‑148 (MQ=255)
                 gTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTcgcg                                                                                                                              >  2:1260860/1‑148 (MQ=255)
                 gTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTcgcg                                                                                                                              >  2:3009668/1‑148 (MQ=255)
                       gctgGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTcc                                                                                                                        <  1:2437480/148‑1 (MQ=255)
                       gctgGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTcc                                                                                                                        <  2:244324/148‑1 (MQ=255)
                        ctgGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTccc                                                                                                                       <  2:711974/148‑1 (MQ=255)
                          gggTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCca                                                                                                                     >  1:2206042/1‑148 (MQ=255)
                           ggTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCcac                                                                                                                    >  1:3120940/1‑148 (MQ=255)
                               aTTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCg                                                                                                                <  1:834274/148‑1 (MQ=255)
                                tttCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGt                                                                                                               >  1:2084484/1‑148 (MQ=255)
                                tttCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGt                                                                                                               <  1:2407948/148‑1 (MQ=255)
                                tttCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGt                                                                                                               <  1:331691/148‑1 (MQ=255)
                                tttCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGt                                                                                                               >  2:630904/1‑148 (MQ=255)
                                 ttCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTc                                                                                                              >  1:2220367/1‑148 (MQ=255)
                                 ttCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTc                                                                                                              >  2:269375/1‑148 (MQ=255)
                                  tCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCa                                                                                                             <  2:2118431/148‑1 (MQ=255)
                                  tCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCa                                                                                                             <  1:1879290/148‑1 (MQ=255)
                                  tCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCa                                                                                                             >  2:783287/1‑148 (MQ=255)
                                  tCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCa                                                                                                             <  2:1946944/148‑1 (MQ=255)
                                    attattAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCAtt                                                                                                           <  1:3212187/148‑1 (MQ=255)
                                     ttattaATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTg                                                                                                          >  2:1970244/1‑148 (MQ=255)
                                      tattaATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGt                                                                                                         >  1:2365492/1‑148 (MQ=255)
                                       attaATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTc                                                                                                        >  1:1166676/1‑148 (MQ=255)
                                        ttaATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCa                                                                                                       <  1:3403381/148‑1 (MQ=255)
                                              tCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAacgacg                                                                                                 >  2:3346497/1‑148 (MQ=255)
                                               cATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGACTTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGt                                                                                                <  2:222437/148‑1 (MQ=255)
                                               cATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGt                                                                                                >  2:2023702/1‑148 (MQ=255)
                                                 ttAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTAc                                                                                              >  2:1745017/1‑148 (MQ=255)
                                                  tAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTAcc                                                                                             >  2:113819/1‑148 (MQ=255)
                                                    aGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCaa                                                                                           >  1:2870871/1‑148 (MQ=255)
                                                    aGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCaa                                                                                           <  1:2577101/148‑1 (MQ=255)
                                                    aGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCaa                                                                                           >  1:1120470/1‑148 (MQ=255)
                                                         tCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGAt                                                                                      <  2:251860/148‑1 (MQ=255)
                                                          ccTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATc                                                                                     >  2:2993485/1‑148 (MQ=255)
                                                           cTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATcc                                                                                    <  2:3019601/148‑1 (MQ=255)
                                                               aaGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTAc                                                                                <  1:428936/148‑1 (MQ=255)
                                                                aGCGAACCATAATATTTATCCATATTTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACa                                                                               <  2:1350488/148‑1 (MQ=255)
                                                                aGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACa                                                                               <  2:947625/148‑1 (MQ=255)
                                                                  agAAACATAATATTTATCCTTAATTTGTGACGATGTATTAAACACGGGGTTAAATTGATCGATTGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACTACGTAAAAACTGATCCTTACaat                                                                             <  2:1419864/147‑2 (MQ=255)
                                                                      ccATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTAc                                                                         >  2:1503977/1‑148 (MQ=255)
                                                                       cATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACg                                                                        >  1:196615/1‑148 (MQ=255)
                                                                          aataTTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaa                                                                     >  1:1443731/1‑148 (MQ=255)
                                                                          aataTTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaa                                                                     >  2:3447525/1‑148 (MQ=255)
                                                                          aataTTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaa                                                                     >  1:3295239/1‑148 (MQ=255)
                                                                          aataTTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaa                                                                     >  2:2040526/1‑148 (MQ=255)
                                                                              tttATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaaaaaa                                                                 >  1:445158/1‑148 (MQ=255)
                                                                              tttATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaaaaaa                                                                 <  1:2120385/148‑1 (MQ=255)
                                                                               ttATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAAt                                                                >  1:1756698/1‑148 (MQ=255)
                                                                                 aTCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTa                                                              <  1:1939349/148‑1 (MQ=255)
                                                                                 aTCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTa                                                              >  1:98166/1‑148 (MQ=255)
                                                                                  tCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTAc                                                             <  2:931600/148‑1 (MQ=255)
                                                                                     aTAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCa                                                          >  2:2955437/1‑148 (MQ=255)
                                                                                       aaTTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAACAAAATTACTCATa                                                        >  2:812276/1‑148 (MQ=255)
                                                                                         tttGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACt                                                      >  2:1023701/1‑148 (MQ=255)
                                                                                              gACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAgg                                                 <  1:1239340/148‑1 (MQ=255)
                                                                                               aCGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGc                                                >  2:2088297/1‑148 (MQ=255)
                                                                                               aCGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGc                                                >  2:317302/1‑148 (MQ=255)
                                                                                                 gATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCAt                                              <  1:731623/148‑1 (MQ=255)
                                                                                                 gATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCAt                                              <  2:1917961/148‑1 (MQ=255)
                                                                                                 gATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCAt                                              <  1:1220835/148‑1 (MQ=255)
                                                                                                 gATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCAt                                              <  1:732427/148‑1 (MQ=255)
                                                                                                  aTGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCAtt                                             <  1:304353/148‑1 (MQ=255)
                                                                                                   tGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTg                                            <  1:1484154/148‑1 (MQ=255)
                                                                                                    gAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTgg                                           <  1:1872755/148‑1 (MQ=255)
                                                                                                      aTTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACGGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCa                                         >  1:2071837/1‑148 (MQ=255)
                                                                                                          aaCACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAAtt                                     >  1:1324430/1‑148 (MQ=255)
                                                                                                           acacGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAAttt                                    >  1:2848205/1‑148 (MQ=255)
                                                                                                              cGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATttatt                                 <  2:2189430/148‑1 (MQ=255)
                                                                                                              cGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATttatt                                 <  2:1541520/148‑1 (MQ=255)
                                                                                                                 ggTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATc                              <  2:2825905/148‑1 (MQ=255)
                                                                                                                 ggTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATc                              <  1:2690827/148‑1 (MQ=255)
                                                                                                                  gTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCt                             >  2:1806841/1‑148 (MQ=255)
                                                                                                                    tAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTgg                           <  2:3505722/148‑1 (MQ=255)
                                                                                                                    tAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTgg                           >  1:3396831/1‑148 (MQ=255)
                                                                                                                       aTTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCaa                        >  1:1417252/1‑148 (MQ=255)
                                                                                                                        ttGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAg                       >  1:477788/1‑148 (MQ=255)
                                                                                                                         tGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGc                      >  1:1447708/1‑148 (MQ=255)
                                                                                                                         tGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGc                      <  1:2506800/148‑1 (MQ=255)
                                                                                                                         tGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGc                      >  1:2230594/1‑148 (MQ=255)
                                                                                                                           aTCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCt                    >  2:53972/1‑148 (MQ=255)
                                                                                                                            tCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTg                   >  1:157968/1‑148 (MQ=255)
                                                                                                                             cGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGa                  <  2:2578449/148‑1 (MQ=255)
                                                                                                                             cGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGa                  >  1:2762914/1‑148 (MQ=255)
                                                                                                                               aTGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGaaa                <  1:3137245/148‑1 (MQ=255)
                                                                                                                                tGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGaaaa               <  1:3223179/148‑1 (MQ=255)
                                                                                                                                    tGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGaa           <  2:1056340/148‑1 (MQ=255)
                                                                                                                                     gCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGaaa          >  2:882387/1‑148 (MQ=255)
                                                                                                                                     gCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGaaa          <  2:477788/148‑1 (MQ=255)
                                                                                                                                         tCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgt      <  1:2023702/148‑1 (MQ=255)
                                                                                                                                         tCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgt      >  1:1002528/1‑148 (MQ=255)
                                                                                                                                           aaCTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATAgttg    >  2:1839165/1‑148 (MQ=255)
                                                                                                                                            aCTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgttgt   >  2:2390096/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
ATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTT  >  CP009273/2474210‑2474498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATCTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAA  >  CP009273/2474204‑2474500
                                                                                                                                                     |                                                                                                                                                   
AATCTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTT                                                                                                                                                     <  SRR19973065.3091943/150‑1 (MQ=60)
   CTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACT                                                                                                                                                  <  SRR19973065.61204/150‑1 (MQ=60)
          GCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGT                                                                                                                                           <  SRR19973065.171432/150‑1 (MQ=60)
            AGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAA                                                                                                                                         >  SRR19973065.1062858/1‑150 (MQ=60)
            AGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAA                                                                                                                                         >  SRR19973065.1063345/1‑150 (MQ=60)
            AGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAA                                                                                                                                         >  SRR19973065.1063455/1‑150 (MQ=60)
            AGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGGTAATTTGAGCGATGGTTGCTGTCAACTTACTCTGGTGTTA                                                                                                                                         >  SRR19973065.3291281/1‑150 (MQ=60)
              AAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACG                                                                                                                                       >  SRR19973065.1545145/1‑150 (MQ=60)
               AATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGA                                                                                                                                      >  SRR19973065.1046671/1‑150 (MQ=60)
                ATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGAT                                                                                                                                     <  SRR19973065.1943399/150‑1 (MQ=60)
                  AGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCTACTTACTATGGTGTAACGATTC                                                                                                                                   >  SRR19973065.2958875/1‑150 (MQ=60)
                  AGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTC                                                                                                                                   <  SRR19973065.1870943/150‑1 (MQ=60)
                            TGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATCTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCC                                                                                                                         <  SRR19973065.1488935/150‑1 (MQ=60)
                             GCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCC                                                                                                                        <  SRR19973065.2452851/150‑1 (MQ=60)
                              CTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCA                                                                                                                       >  SRR19973065.2220156/1‑150 (MQ=60)
                               TGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCAC                                                                                                                      <  SRR19973065.726879/150‑1 (MQ=60)
                               TGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCAC                                                                                                                      >  SRR19973065.3139950/1‑150 (MQ=60)
                               TGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCAC                                                                                                                      >  SRR19973065.3139963/1‑150 (MQ=60)
                                 GGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAG                                                                                                                    >  SRR19973065.1132561/1‑150 (MQ=60)
                                    TATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGT                                                                                                                 >  SRR19973065.2097847/1‑150 (MQ=60)
                                     ATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTC                                                                                                                >  SRR19973065.2234537/1‑150 (MQ=60)
                                     ATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTC                                                                                                                >  SRR19973065.3315582/1‑150 (MQ=60)
                                     ATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTC                                                                                                                <  SRR19973065.839717/150‑1 (MQ=60)
                                     ATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTC                                                                                                                >  SRR19973065.1569702/1‑150 (MQ=60)
                                     ATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTC                                                                                                                >  SRR19973065.224088/1‑150 (MQ=60)
                                      TTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCA                                                                                                               <  SRR19973065.2423102/150‑1 (MQ=60)
                                      TTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCA                                                                                                               <  SRR19973065.334015/150‑1 (MQ=60)
                                      TTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCA                                                                                                               <  SRR19973065.334014/150‑1 (MQ=60)
                                        TCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATT                                                                                                             <  SRR19973065.1890364/150‑1 (MQ=60)
                                        TCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATT                                                                                                             <  SRR19973065.1891662/150‑1 (MQ=60)
                                          ATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGT                                                                                                           <  SRR19973065.298406/150‑1 (MQ=60)
                                          ATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGT                                                                                                           >  SRR19973065.2380228/1‑150 (MQ=60)
                                          ATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGT                                                                                                           <  SRR19973065.3231508/150‑1 (MQ=60)
                                           TTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTC                                                                                                          >  SRR19973065.1174122/1‑150 (MQ=60)
                                              TTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAAC                                                                                                       <  SRR19973065.3423800/150‑1 (MQ=60)
                                              TTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAAC                                                                                                       <  SRR19973065.3424179/150‑1 (MQ=60)
                                              TTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAAC                                                                                                       <  SRR19973065.3057062/150‑1 (MQ=60)
                                               TAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACG                                                                                                      >  SRR19973065.2124875/1‑150 (MQ=60)
                                                 ATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGAC                                                                                                    <  SRR19973065.384393/150‑1 (MQ=60)
                                                    TCATTAAGAACTCGTTATAGCGAAACATTATATTTATCCATTATTTGTGACGATGTATTAAACACGGGGTTAAATTGATCGTTGTTTGCTGTCAACTTAATATGGTGTAACGATTGGAGTCGTCCCAACAGCGTAATTGTCAACGACGTA                                                                                                 <  SRR19973065.2381068/150‑1 (MQ=60)
                                                    TCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTA                                                                                                 <  SRR19973065.1753325/150‑1 (MQ=60)
                                                      ATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACC                                                                                               >  SRR19973065.666080/1‑150 (MQ=60)
                                                        TAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAA                                                                                             >  SRR19973065.2917883/1‑150 (MQ=60)
                                                        TAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAA                                                                                             >  SRR19973065.3198915/1‑150 (MQ=60)
                                                        TAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAA                                                                                             >  SRR19973065.2888470/1‑150 (MQ=60)
                                                        TAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAA                                                                                             >  SRR19973065.1127773/1‑150 (MQ=60)
                                                         AAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAAC                                                                                            >  SRR19973065.3073199/1‑150 (MQ=60)
                                                         AAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAAC                                                                                            >  SRR19973065.2654205/1‑150 (MQ=60)
                                                          AGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACT                                                                                           <  SRR19973065.2593174/150‑1 (MQ=60)
                                                           GAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTG                                                                                          >  SRR19973065.863762/1‑150 (MQ=60)
                                                                CCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTTAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCAAAGCGTCATTGTCAACGACGTACCAACTGATCCT                                                                                     <  SRR19973065.164771/150‑1 (MQ=60)
                                                                     AAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAA                                                                                <  SRR19973065.431913/150‑1 (MQ=60)
                                                                       GCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGG                                                                              <  SRR19973065.2870101/150‑1 (MQ=60)
                                                                           ACCATGATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG                                                                          >  SRR19973065.198096/1‑150 (MQ=60)
                                                                           ACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG                                                                          >  SRR19973065.198146/1‑150 (MQ=60)
                                                                              ATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAA                                                                       >  SRR19973065.1453072/1‑150 (MQ=60)
                                                                              ATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAA                                                                       >  SRR19973065.1235557/1‑150 (MQ=60)
                                                                              ATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAA                                                                       >  SRR19973065.1453564/1‑150 (MQ=60)
                                                                              ATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAA                                                                       >  SRR19973065.3315305/1‑150 (MQ=60)
                                                                                  TATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAA                                                                   >  SRR19973065.448235/1‑150 (MQ=60)
                                                                                  TATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAA                                                                   >  SRR19973065.448239/1‑150 (MQ=60)
                                                                                  TATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAA                                                                   >  SRR19973065.1061943/1‑150 (MQ=60)
                                                                                   ATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAAT                                                                  >  SRR19973065.1768245/1‑150 (MQ=60)
                                                                                   ATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAAT                                                                  >  SRR19973065.1768254/1‑150 (MQ=60)
                                                                                    TTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATT                                                                 <  SRR19973065.2134034/150‑1 (MQ=60)
                                                                                     TTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTA                                                                >  SRR19973065.99070/1‑150 (MQ=60)
                                                                                       ATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACT                                                              <  SRR19973065.1952036/150‑1 (MQ=60)
                                                                                              ATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTC                                                       >  SRR19973065.2438514/1‑150 (MQ=60)
                                                                                                   TGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGC                                                  >  SRR19973065.2233493/1‑150 (MQ=60)
                                                                                                    GACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCA                                                 <  SRR19973065.1247479/150‑1 (MQ=60)
                                                                                                       GATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTG                                              <  SRR19973065.2543846/150‑1 (MQ=60)
                                                                                                       GATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTG                                              <  SRR19973065.737326/150‑1 (MQ=60)
                                                                                                       GATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTG                                              <  SRR19973065.737127/150‑1 (MQ=60)
                                                                                                       GATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTG                                              <  SRR19973065.736520/150‑1 (MQ=60)
                                                                                                       GATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTG                                              <  SRR19973065.1228780/150‑1 (MQ=60)
                                                                                                       GATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTG                                              <  SRR19973065.1421354/150‑1 (MQ=60)
                                                                                                        ATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGG                                             <  SRR19973065.306370/150‑1 (MQ=60)
                                                                                                         TGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGC                                            <  SRR19973065.1493620/150‑1 (MQ=60)
                                                                                                          GAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACGGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCA                                           >  SRR19973065.2085055/1‑150 (MQ=60)
                                                                                                          GAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCA                                           >  SRR19973065.2086144/1‑150 (MQ=60)
                                                                                                          GAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCA                                           <  SRR19973065.1883019/150‑1 (MQ=60)
                                                                                                          GAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCA                                           <  SRR19973065.1885088/150‑1 (MQ=60)
                                                                                                              TAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATT                                       >  SRR19973065.1981779/1‑150 (MQ=60)
                                                                                                              TAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATT                                       >  SRR19973065.1333187/1‑150 (MQ=60)
                                                                                                               AAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTT                                      >  SRR19973065.2865727/1‑150 (MQ=60)
                                                                                                                    CGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTAT                                 <  SRR19973065.2472058/150‑1 (MQ=60)
                                                                                                                      GGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCT                               <  SRR19973065.128685/150‑1 (MQ=60)
                                                                                                                       GGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTG                              <  SRR19973065.2707532/150‑1 (MQ=60)
                                                                                                                        GTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGG                             >  SRR19973065.3417597/1‑150 (MQ=60)
                                                                                                                          TAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCA                           >  SRR19973065.2211715/1‑150 (MQ=60)
                                                                                                                           AAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAA                          >  SRR19973065.1426507/1‑150 (MQ=60)
                                                                                                                           AAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAA                          >  SRR19973065.1427322/1‑150 (MQ=60)
                                                                                                                            AATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAG                         >  SRR19973065.481047/1‑150 (MQ=60)
                                                                                                                            AATTGATCGATGGTTGCTGTCAACTAACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACCCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAG                         >  SRR19973065.2710531/1‑150 (MQ=60)
                                                                                                                             ATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGC                        >  SRR19973065.465112/1‑150 (MQ=60)
                                                                                                                             ATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGC                        >  SRR19973065.2244791/1‑150 (MQ=60)
                                                                                                                             ATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGC                        >  SRR19973065.1457063/1‑150 (MQ=60)
                                                                                                                               TGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCT                      <  SRR19973065.2522551/150‑1 (MQ=60)
                                                                                                                                GATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTG                     >  SRR19973065.159274/1‑150 (MQ=60)
                                                                                                                                GATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATAGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATGTATTATCTGGCAAGCCTG                     >  SRR19973065.1188068/1‑150 (MQ=60)
                                                                                                                                 ATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGA                    >  SRR19973065.1015789/1‑150 (MQ=60)
                                                                                                                                 ATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGA                    >  SRR19973065.2780079/1‑150 (MQ=60)
                                                                                                                                    GATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAA                 >  SRR19973065.2065507/1‑150 (MQ=60)
                                                                                                                                     ATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAAC                <  SRR19973065.3156324/150‑1 (MQ=60)
                                                                                                                                      TGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACG               <  SRR19973065.3242540/150‑1 (MQ=60)
                                                                                                                                      TGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACG               <  SRR19973065.3242536/150‑1 (MQ=60)
                                                                                                                                       GGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGA              >  SRR19973065.1094657/1‑150 (MQ=60)
                                                                                                                                       GGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGA              >  SRR19973065.2855034/1‑150 (MQ=60)
                                                                                                                                         TTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAA            <  SRR19973065.788489/150‑1 (MQ=60)
                                                                                                                                             TGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGT        >  SRR19973065.2190689/1‑150 (MQ=60)
                                                                                                                                             TGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGT        >  SRR19973065.1009185/1‑150 (MQ=60)
                                                                                                                                               TCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTG      <  SRR19973065.2036714/150‑1 (MQ=60)
                                                                                                                                                 AACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTT    >  SRR19973065.445822/1‑150 (MQ=60)
                                                                                                                                                 AACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGACATCGTTGTT    >  SRR19973065.2280435/1‑150 (MQ=60)
                                                                                                                                                  ACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTA   >  SRR19973065.2714820/1‑150 (MQ=60)
                                                                                                                                                   CTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAA  >  SRR19973065.607938/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
AATCTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAA  >  CP009273/2474204‑2474500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑