Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F247 I0 R1	1496	299.8	9582018	97.8%	9371213	149.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,474,353	G→T	88.7%	Q435K (CAG→AAG)	emrY ←	putative multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,474,353	0	G	T	88.7%	700.3 / 89.9	275	Q435K (CAG→AAG)	emrY	putative multidrug efflux system
Reads supporting (aligned to +/- strand): ref base G (17/14); new base T (122/120); total (140/135)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

Only 100 of 277 total aligned reads displayed.

CTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAG  >  CP009273/2474207‑2474501

  cATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTa                                                                                                                                                  <  1:2426435/149‑1 (MQ=255)

    tttGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATg                                                                                                                                                >  1:324177/1‑149 (MQ=255)

       gCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGgtg                                                                                                                                             >  2:1286091/1‑149 (MQ=255)

          gAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATACTTTGTGACGATGAATTAAACACGGGGTGAACTGGTTCGATGGTTGCTGTCAAGTTACTGTGGTGTaa                                                                                                                                          >  2:2656441/1‑149 (MQ=255)

            aaTAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACg                                                                                                                                        >  1:4256450/1‑149 (MQ=255)

             aTAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGa                                                                                                                                       >  2:162810/1‑149 (MQ=255)

              tAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGAt                                                                                                                                      >  2:2862065/1‑149 (MQ=255)

               aGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGAtt                                                                                                                                     >  1:930486/1‑149 (MQ=255)

                 aaaGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTcg                                                                                                                                   >  2:2273633/1‑149 (MQ=255)

                  aaGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTcgc                                                                                                                                  <  1:3557280/149‑1 (MQ=255)

                     tGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGcgtc                                                                                                                               >  2:4718629/1‑149 (MQ=255)

                     tGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGcgtc                                                                                                                               >  2:2205007/1‑149 (MQ=255)

                        ctgctgGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCgtcgtc                                                                                                                            <  2:3390428/149‑1 (MQ=255)

                        ctgctgGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCgtcgtc                                                                                                                            <  2:2620334/149‑1 (MQ=255)

                         tgctgGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTcc                                                                                                                           <  2:1729617/149‑1 (MQ=255)

                           ctgGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTcccc                                                                                                                         <  1:1640188/149‑1 (MQ=255)

                                   tttCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTc                                                                                                                 <  2:396490/149‑1 (MQ=255)

                                      cATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCAtt                                                                                                              >  2:1445907/1‑149 (MQ=255)

                                       attattAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTg                                                                                                             <  1:2723022/149‑1 (MQ=255)

                                         tattaATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTc                                                                                                           >  1:3350748/1‑149 (MQ=255)

                                          attaATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCa                                                                                                          >  2:3668309/1‑149 (MQ=255)

                                           ttaATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCaa                                                                                                         <  2:4111776/149‑1 (MQ=255)

                                                   aTTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTAc                                                                                                 <  2:3193233/149‑1 (MQ=255)

                                                     tAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCa                                                                                               <  1:3219781/149‑1 (MQ=255)

                                                       aGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAAc                                                                                             <  1:3820295/149‑1 (MQ=255)

                                                        gAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTcccc                                                                                                                         >  2:1905424/1‑120 (MQ=255)

                                                        gAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTcccc                                                                                                                         <  1:1905424/120‑1 (MQ=255)

                                                         aaCTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGAACCAACTg                                                                                           <  2:4735975/149‑1 (MQ=255)

                                                         aaCTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGAACCAACTg                                                                                           <  2:2338566/149‑1 (MQ=255)

                                                         aaCTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGAACCAACTg                                                                                           <  2:3536803/149‑1 (MQ=255)

                                                          aCTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGa                                                                                          <  1:1441778/149‑1 (MQ=255)

                                                           cTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGAt                                                                                         >  1:2484408/1‑149 (MQ=255)

                                                              cTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCt                                                                                      <  1:2946637/149‑1 (MQ=255)

                                                                   aGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACAAACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACaa                                                                                 >  2:505657/1‑149 (MQ=255)

                                                                    gCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTATCAACGACGTACCAACTGATCCTTACAAg                                                                                >  1:308383/1‑149 (MQ=255)

                                                                      gAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGt                                                                              >  2:3642351/1‑149 (MQ=255)

                                                                      gAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGt                                                                              <  1:4480020/149‑1 (MQ=255)

                                                                      gAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGt                                                                              >  1:4561620/1‑149 (MQ=255)

                                                                      gAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTAGGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTCCAAGGt                                                                              >  2:1252493/1‑149 (MQ=255)

                                                                       aaCCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTa                                                                             >  1:1807374/1‑149 (MQ=255)

                                                                       aaCCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTa                                                                             >  1:711955/1‑149 (MQ=255)

                                                                          cATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGa                                                                          >  2:3029608/1‑149 (MQ=255)

                                                                            taataTTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGaaa                                                                        >  2:1650516/1‑149 (MQ=255)

                                                                                  ttATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAATGACGTACCAACTGATCCTTACAAGGTACGAAAAAAAtt                                                                  <  2:2350548/149‑1 (MQ=255)

                                                                                  ttATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAAtt                                                                  >  2:1369275/1‑149 (MQ=255)

                                                                                  ttATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAAtt                                                                  <  2:4331425/149‑1 (MQ=255)

                                                                                  ttATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAAtt                                                                  <  1:593237/149‑1 (MQ=255)

                                                                                    aTCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTAc                                                                >  1:4076404/1‑149 (MQ=255)

                                                                                        aTAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCAt                                                            >  1:763201/1‑149 (MQ=255)

                                                                                         tAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATa                                                           >  2:2043661/1‑149 (MQ=255)

                                                                                           aTTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACt                                                         <  2:3292350/149‑1 (MQ=255)

                                                                                              tgtgACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGa                                                      <  1:1169352/149‑1 (MQ=255)

                                                                                                 gACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGc                                                   <  2:4052994/149‑1 (MQ=255)

                                                                                                  aCGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCa                                                  <  2:3492015/149‑1 (MQ=255)

                                                                                                   cGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCAt                                                 <  1:317668/149‑1 (MQ=255)

                                                                                                      tGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTgg                                              >  2:922776/1‑149 (MQ=255)

                                                                                                       gAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGc                                             <  1:3435596/149‑1 (MQ=255)

                                                                                                       gAATTAAACACGGGGTTAAATTGATCGATGGTTACTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGc                                             >  1:1023638/1‑149 (MQ=255)

                                                                                                        aaTTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCa                                            >  1:1820448/1‑149 (MQ=255)

                                                                                                          ttAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCaaa                                          >  1:3515301/1‑149 (MQ=255)

                                                                                                          ttAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCaaa                                          <  1:1297044/149‑1 (MQ=255)

                                                                                                            aaaCACGGGTTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAAtt                                        <  1:4326929/149‑1 (MQ=255)

                                                                                                            aaaCACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAAtt                                        <  1:3423542/149‑1 (MQ=255)

                                                                                                             aaCACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAAttt                                       >  1:694390/1‑149 (MQ=255)

                                                                                                              acacGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATtta                                      <  2:3359207/149‑1 (MQ=255)

                                                                                                                  ggggTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTtattat                                  <  2:2044738/149‑1 (MQ=255)

                                                                                                                  ggggTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTtattat                                  <  1:495313/149‑1 (MQ=255)

                                                                                                                   gggTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATc                                 <  2:543347/149‑1 (MQ=255)

                                                                                                                    ggTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCt                                >  1:2282102/1‑149 (MQ=255)

                                                                                                                    ggTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCt                                <  2:1993789/149‑1 (MQ=255)

                                                                                                                      ttAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTgg                              >  1:3878215/1‑149 (MQ=255)

                                                                                                                      ttAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTgg                              >  1:1726584/1‑149 (MQ=255)

                                                                                                                      ttAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTgg                              <  2:3789952/149‑1 (MQ=255)

                                                                                                                      ttAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTgg                              <  2:3789990/149‑1 (MQ=255)

                                                                                                                        aaaTTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCa                            >  1:1759021/1‑149 (MQ=255)

                                                                                                                          aTTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAg                          <  2:4764097/149‑1 (MQ=255)

                                                                                                                           ttGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGc                         <  2:324177/149‑1 (MQ=255)

                                                                                                                             gATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCt                       >  1:4694938/1‑149 (MQ=255)

                                                                                                                              aTCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTg                      >  1:4732249/1‑149 (MQ=255)

                                                                                                                              aTCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTg                      >  1:987405/1‑149 (MQ=255)

                                                                                                                               tCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGa                     >  2:2315474/1‑149 (MQ=255)

                                                                                                                                cGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGaa                    >  2:996726/1‑149 (MQ=255)

                                                                                                                                  aTGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGaaaa                  >  2:2939766/1‑149 (MQ=255)

                                                                                                                                   tGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAAc                 >  1:1061680/1‑149 (MQ=255)

                                                                                                                                   tGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAAc                 >  2:613124/1‑149 (MQ=255)

                                                                                                                                     gTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGa               <  2:938610/149‑1 (MQ=255)

                                                                                                                                     gTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGa               >  1:2767289/1‑149 (MQ=255)

                                                                                                                                     gTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGa               >  1:374766/1‑149 (MQ=255)

                                                                                                                                      ttGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGaa              <  1:534858/149‑1 (MQ=255)

                                                                                                                                       tGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGaaa             <  2:4166409/149‑1 (MQ=255)

                                                                                                                                       tGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGaaa             <  1:2714627/149‑1 (MQ=255)

                                                                                                                                          tGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCg          >  1:1108564/1‑149 (MQ=255)

                                                                                                                                           gTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgt         >  1:4611741/1‑149 (MQ=255)

                                                                                                                                           gTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgt         <  2:2929677/149‑1 (MQ=255)

                                                                                                                                            tCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgtt        <  2:436803/149‑1 (MQ=255)

                                                                                                                                            tCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgtt        <  2:1342615/149‑1 (MQ=255)

                                                                                                                                               aCTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgttgtt     >  2:4127334/1‑149 (MQ=255)

                                                                                                                                               aCTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgttgtt     >  1:1223388/1‑149 (MQ=255)

                                                                                                                                               aCTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCgttgtt     <  2:1157761/149‑1 (MQ=255)

                                                                                                                                                  gACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAg  <  1:673362/149‑1 (MQ=255)

CTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAG  >  CP009273/2474207‑2474501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AATCTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGG  >  CP009273/2474204‑2474502

AATCTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTT                                                                                                                                                       >  SRR19973071.3391275/1‑150 (MQ=60)

  TCTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTAC                                                                                                                                                     >  SRR19973071.1863451/1‑150 (MQ=60)

  TCTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTAC                                                                                                                                                     >  SRR19973071.138754/1‑150 (MQ=60)

  TCTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTAC                                                                                                                                                     <  SRR19973071.3441516/150‑1 (MQ=60)

     CATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTAT                                                                                                                                                  <  SRR19973071.2435213/150‑1 (MQ=60)

      ATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATG                                                                                                                                                 >  SRR19973071.325283/1‑150 (MQ=60)

            AGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAA                                                                                                                                           >  SRR19973071.1707266/1‑150 (MQ=60)

              AAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACG                                                                                                                                         >  SRR19973071.4271550/1‑150 (MQ=60)

                 TAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATT                                                                                                                                      >  SRR19973071.933871/1‑150 (MQ=60)

                 TAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATT                                                                                                                                      >  SRR19973071.1686382/1‑150 (MQ=60)

                     AAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCG                                                                                                                                  <  SRR19973071.3569679/150‑1 (MQ=60)

                     AAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCG                                                                                                                                  <  SRR19973071.3569556/150‑1 (MQ=60)

                       GTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTC                                                                                                                                <  SRR19973071.3248209/150‑1 (MQ=60)

                            TGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCC                                                                                                                           >  SRR19973071.4032228/1‑150 (MQ=60)

                              ggagttcagacgtgtgctcttccgatctAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCA                                                                                                                         <  SRR19973071.1912416/122‑1 (MQ=60)

                              CTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCA                                                                                                                         <  SRR19973071.1646148/150‑1 (MQ=60)

                                GGTTTATTTCATTATTAATTTCATTAAGAACTACTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACA                                                                                                                       <  SRR19973071.1888674/150‑1 (MQ=60)

                                GGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACA                                                                                                                       <  SRR19973071.3175031/150‑1 (MQ=60)

                                GGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACA                                                                                                                       <  SRR19973071.3174986/150‑1 (MQ=60)

                                 GGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAG                                                                                                                      >  SRR19973071.748553/1‑150 (MQ=60)

                                  GTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGC                                                                                                                     >  SRR19973071.2843878/1‑150 (MQ=60)

                                   TTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCG                                                                                                                    <  SRR19973071.2445252/150‑1 (MQ=60)

                                      TTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCA                                                                                                                 <  SRR19973071.1950176/150‑1 (MQ=60)

                                         CATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTG                                                                                                              <  SRR19973071.459574/150‑1 (MQ=60)

                                          ATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGT                                                                                                             <  SRR19973071.2732790/150‑1 (MQ=60)

                                          ATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGT                                                                                                             <  SRR19973071.2732708/150‑1 (MQ=60)

                                           TTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTC                                                                                                            >  SRR19973071.3362638/1‑150 (MQ=60)

                                           TTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTC                                                                                                            >  SRR19973071.4238228/1‑150 (MQ=60)

                                              TTAATTTAATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAAC                                                                                                         <  SRR19973071.4080812/150‑1 (MQ=60)

                                                 ATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGAC                                                                                                      >  SRR19973071.1176508/1‑150 (MQ=60)

                                                    TCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTA                                                                                                   >  SRR19973071.695915/1‑150 (MQ=60)

                                                     CATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTAC                                                                                                  <  SRR19973071.4731328/150‑1 (MQ=60)

                                                        TAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAA                                                                                               <  SRR19973071.3231096/150‑1 (MQ=60)

                                                        TAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAA                                                                                               <  SRR19973071.3231086/150‑1 (MQ=60)

                                                         AAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAAC                                                                                              <  SRR19973071.3302831/150‑1 (MQ=60)

                                                          AGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACT                                                                                             <  SRR19973071.3833850/150‑1 (MQ=60)

                                                          AGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACT                                                                                             <  SRR19973071.3833047/150‑1 (MQ=60)

                                                          AGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACT                                                                                             >  SRR19973071.1784038/1‑150 (MQ=60)

                                                          AGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACT                                                                                             >  SRR19973071.1783606/1‑150 (MQ=60)

                                                          AGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACT                                                                                             >  SRR19973071.2941339/1‑150 (MQ=60)

                                                          AGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACT                                                                                             <  SRR19973071.3881049/150‑1 (MQ=60)

                                                          AGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACT                                                                                             >  SRR19973071.541039/1‑150 (MQ=60)

                                                            AACTCCTGAAAGCGAACCATAATATTTATCCCTAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGA                                                                                           >  SRR19973071.4642905/1‑150 (MQ=60)

                                                            AACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGA                                                                                           >  SRR19973071.3311009/1‑150 (MQ=60)

                                                             ACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGAT                                                                                          >  SRR19973071.2493450/1‑150 (MQ=60)

                                                             ACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGAT                                                                                          <  SRR19973071.1447062/150‑1 (MQ=60)

                                                             ACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGAT                                                                                          <  SRR19973071.1070699/150‑1 (MQ=60)

                                                                 CTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTT                                                                                      <  SRR19973071.2957194/150‑1 (MQ=60)

                                                                  TGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTA                                                                                     >  SRR19973071.1271444/1‑150 (MQ=60)

                                                                   GAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTAC                                                                                    >  SRR19973071.593937/1‑150 (MQ=60)

                                                                      AGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTATCAACGACGTACCAACTGATCCTTACAAG                                                                                 >  SRR19973071.309465/1‑150 (MQ=60)

                                                                        CGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGT                                                                               >  SRR19973071.4577839/1‑150 (MQ=60)

                                                                        CGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGT                                                                               >  SRR19973071.4577899/1‑150 (MQ=60)

                                                                        CGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGT                                                                               >  SRR19973071.4577871/1‑150 (MQ=60)

                                                                         GAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTA                                                                              <  SRR19973071.1186284/150‑1 (MQ=60)

                                                                         GAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTA                                                                              <  SRR19973071.4495916/150‑1 (MQ=60)

                                                                         GAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTA                                                                              >  SRR19973071.1814109/1‑150 (MQ=60)

                                                                         GAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTA                                                                              >  SRR19973071.714100/1‑150 (MQ=60)

                                                                         GAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTA                                                                              >  SRR19973071.714447/1‑150 (MQ=60)

                                                                           ACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACG                                                                            <  SRR19973071.1156611/150‑1 (MQ=60)

                                                                            CCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGA                                                                           >  SRR19973071.1302559/1‑150 (MQ=60)

                                                                              ATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAA                                                                         >  SRR19973071.2279755/1‑150 (MQ=60)

                                                                                   ATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAAT                                                                    >  SRR19973071.3399398/1‑150 (MQ=60)

                                                                                     TTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTA                                                                  <  SRR19973071.595731/150‑1 (MQ=60)

                                                                                     TTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTA                                                                  <  SRR19973071.595448/150‑1 (MQ=60)

                                                                                      TATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTAC                                                                 >  SRR19973071.3631463/1‑150 (MQ=60)

                                                                                      TATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTAC                                                                 >  SRR19973071.4090859/1‑150 (MQ=60)

                                                                                       ATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACT                                                                >  SRR19973071.2470466/1‑150 (MQ=60)

                                                                                       ATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACT                                                                >  SRR19973071.4686484/1‑150 (MQ=60)

                                                                                         CCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCA                                                              <  SRR19973071.4567356/150‑1 (MQ=60)

                                                                                          CATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCAT                                                             <  SRR19973071.148734/150‑1 (MQ=60)

                                                                                          CATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCAT                                                             >  SRR19973071.765792/1‑150 (MQ=60)

                                                                                            TAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTATCGATTCGCGTCGTCCCCTCAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATAC                                                           <  SRR19973071.413530/150‑1 (MQ=60)

                                                                                             AATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACT                                                          <  SRR19973071.3712217/150‑1 (MQ=60)

                                                                                                 TGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAG                                                      >  SRR19973071.4429284/1‑150 (MQ=60)

                                                                                                 TGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAG                                                      <  SRR19973071.1173388/150‑1 (MQ=60)

                                                                                                    GACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCA                                                   >  SRR19973071.515485/1‑150 (MQ=60)

                                                                                                      CGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATT                                                 <  SRR19973071.318761/150‑1 (MQ=60)

                                                                                                       GATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTG                                                >  SRR19973071.3223993/1‑150 (MQ=60)

                                                                                                         TGAATTAAACACGGGGTTAAATTGATCGATGGTTACTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGC                                              >  SRR19973071.1027344/1‑150 (MQ=60)

                                                                                                          GAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCA                                             >  SRR19973071.1827213/1‑150 (MQ=60)

                                                                                                          GAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCA                                             >  SRR19973071.1827138/1‑150 (MQ=60)

                                                                                                          GAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCA                                             <  SRR19973071.3447733/150‑1 (MQ=60)

                                                                                                          GAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCA                                             >  SRR19973071.1827223/1‑150 (MQ=60)

                                                                                                          GAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCA                                             <  SRR19973071.3447685/150‑1 (MQ=60)

                                                                                                          GAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCA                                             <  SRR19973071.3447677/150‑1 (MQ=60)

                                                                                                           AATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAA                                            >  SRR19973071.430983/1‑150 (MQ=60)

                                                                                                            ATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAA                                           >  SRR19973071.3527758/1‑150 (MQ=60)

                                                                                                            ATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAA                                           >  SRR19973071.3527534/1‑150 (MQ=60)

                                                                                                            ATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAA                                           >  SRR19973071.3527630/1‑150 (MQ=60)

                                                                                                             TTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAAT                                          <  SRR19973071.1301777/150‑1 (MQ=60)

                                                                                                              TAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATT                                         <  SRR19973071.775361/150‑1 (MQ=60)

                                                                                                               AAACACGGGTTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTT                                        <  SRR19973071.4342204/150‑1 (MQ=60)

                                                                                                               AAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTT                                        <  SRR19973071.3435658/150‑1 (MQ=60)

                                                                                                               AAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTT                                        <  SRR19973071.1038557/150‑1 (MQ=60)

                                                                                                               AAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTT                                        >  SRR19973071.697184/1‑150 (MQ=60)

                                                                                                               AAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTT                                        >  SRR19973071.696849/1‑150 (MQ=60)

                                                                                                                  CACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATT                                     >  SRR19973071.4292387/1‑150 (MQ=60)

                                                                                                                   ACGGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTA                                    <  SRR19973071.2303627/150‑1 (MQ=60)

                                                                                                                     GGGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATC                                  <  SRR19973071.497153/150‑1 (MQ=60)

                                                                                                                      GTGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCT                                 <  SRR19973071.3080491/150‑1 (MQ=60)

                                                                                                                      GGGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCT                                 >  SRR19973071.2290473/1‑150 (MQ=60)

                                                                                                                       GGTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTG                                >  SRR19973071.503751/1‑150 (MQ=60)

                                                                                                                        GTTAATTTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGG                               <  SRR19973071.990715/150‑1 (MQ=60)

                                                                                                                        GTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGG                               >  SRR19973071.1733012/1‑150 (MQ=60)

                                                                                                                        GTTAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGG                               >  SRR19973071.3891925/1‑150 (MQ=60)

                                                                                                                          TAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCA                             >  SRR19973071.3021073/1‑150 (MQ=60)

                                                                                                                          TAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCA                             >  SRR19973071.743669/1‑150 (MQ=60)

                                                                                                                          TAAATTGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCA                             >  SRR19973071.1765585/1‑150 (MQ=60)

                                                                                                                               TGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCT                        >  SRR19973071.4711612/1‑150 (MQ=60)

                                                                                                                               TGATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCT                        >  SRR19973071.4711728/1‑150 (MQ=60)

                                                                                                                                GATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTG                       >  SRR19973071.4749026/1‑150 (MQ=60)

                                                                                                                                GATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTG                       >  SRR19973071.990996/1‑150 (MQ=60)

                                                                                                                                GATCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTG                       >  SRR19973071.4749004/1‑150 (MQ=60)

                                                                                                                                  TCGATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAA                     <  SRR19973071.1764575/150‑1 (MQ=60)

                                                                                                                                    GATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAA                   >  SRR19973071.1137958/1‑150 (MQ=60)

                                                                                                                                     ATGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAAC                  >  SRR19973071.1065475/1‑150 (MQ=60)

                                                                                                                                      TGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACG                 <  SRR19973071.815961/150‑1 (MQ=60)

                                                                                                                                      TGGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACG                 >  SRR19973071.3423148/1‑150 (MQ=60)

                                                                                                                                       GGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGA                >  SRR19973071.375972/1‑150 (MQ=60)

                                                                                                                                       GGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGA                >  SRR19973071.375950/1‑150 (MQ=60)

                                                                                                                                       GGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGA                >  SRR19973071.1553894/1‑150 (MQ=60)

                                                                                                                                       GGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGA                >  SRR19973071.2777046/1‑150 (MQ=60)

                                                                                                                                       GGTTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGA                >  SRR19973071.2627412/1‑150 (MQ=60)

                                                                                                                                         TTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAA              <  SRR19973071.2727879/150‑1 (MQ=60)

                                                                                                                                         TTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAA              <  SRR19973071.2727624/150‑1 (MQ=60)

                                                                                                                                         TTGCTGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAA              <  SRR19973071.536857/150‑1 (MQ=60)

                                                                                                                                          TGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAAT             <  SRR19973071.2724291/150‑1 (MQ=60)

                                                                                                                                            CTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCG           >  SRR19973071.1112466/1‑150 (MQ=60)

                                                                                                                                             TGTCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGT          >  SRR19973071.4628185/1‑150 (MQ=60)

                                                                                                                                             TGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGT          >  SRR19973071.1073937/1‑150 (MQ=60)

                                                                                                                                               TCAACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTG        <  SRR19973071.2702699/150‑1 (MQ=60)

                                                                                                                                                 AACTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTT      >  SRR19973071.1227662/1‑150 (MQ=60)

                                                                                                                                                   CTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAA    >  SRR19973071.644902/1‑150 (MQ=60)

                                                                                                                                                   CTTACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAA    >  SRR19973071.3926083/1‑150 (MQ=60)

                                                                                                                                                     TACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGG  <  SRR19973071.3502705/150‑1 (MQ=60)

                                                                                                                                                     TACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGG  <  SRR19973071.3502706/150‑1 (MQ=60)

                                                                                                                                                     GACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGG  <  SRR19973071.675784/150‑1 (MQ=60)

                                                                                                                                                     GACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTTACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGG  <  SRR19973071.675655/150‑1 (MQ=60)

AATCTCATTTGCAGAAATAGAAAGTGACTGCTGGGTTATTTCATTATTAATTTCATTAAGAACTCCTGAAAGCGAACCATAATATTTATCCATAATTTGTGACGATGAATTAAACACGGGGTTAAATTGATCGATGGTTGCTGTCAACTGACTATGGTGTAACGATTCGCGTCGTCCCCACAGCGTCATTGTCAACGACGTACCAACTGATCCTGACAAGGTACGAAAAAAATTACTCATACTCGAGGCATTGGCAAATTTATTATCTGGCAAGCCTGAAAACGAAATCGTTGTTAAGG  >  CP009273/2474204‑2474502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`