Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F488 I0 R1	1275	300.1	10328444	91.0%	9398884	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,258,871	G→T	100%	L173I (CTC→ATC)	tdcB ←	L‑threonine dehydratase, catabolic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,258,871	0	G	T	98.4%	805.5 / 2.4	251	L173I (CTC→ATC)	tdcB	L‑threonine dehydratase, catabolic
Reads supporting (aligned to +/- strand): ref base G (3/1); new base T (130/116); total (133/118)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 255 total aligned reads displayed.

AGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACG  >  CP009273/3258724‑3259016

aGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGatat                                                                                                                                                 <  2:4061498/150‑1 (MQ=255)

     aaaGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTcc                                                                                                                                            >  2:697808/1‑150 (MQ=255)

       agaagCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCAt                                                                                                                                          >  1:2362986/1‑150 (MQ=255)

        gaagCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATa                                                                                                                                         >  2:502580/1‑150 (MQ=255)

          agCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAAt                                                                                                                                       >  1:5022535/1‑150 (MQ=255)

                cATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAg                                                                                                                                 <  2:2003432/150‑1 (MQ=255)

                   gCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGAcc                                                                                                                              >  1:1577833/1‑150 (MQ=255)

                   gCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGAcc                                                                                                                              >  1:788735/1‑150 (MQ=255)

                     cGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCaa                                                                                                                            >  1:2448759/1‑150 (MQ=255)

                      gTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAAt                                                                                                                           >  2:2330402/1‑150 (MQ=255)

                      gTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAAt                                                                                                                           >  1:3769347/1‑150 (MQ=255)

                       tGAACGTTTTCAGACTGTACGCCAAGAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATc                                                                                                                          <  2:2040477/150‑1 (MQ=255)

                         aaCGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGt                                                                                                                        >  1:4861772/1‑150 (MQ=255)

                          aCGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGtt                                                                                                                       <  2:158226/150‑1 (MQ=255)

                             ttttCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATACTTTCCAGACCAATCGTTccc                                                                                                                    >  2:3683639/1‑150 (MQ=255)

                              tttCAGACTGTACGCAAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCt                                                                                                                   <  1:2540747/150‑1 (MQ=255)

                               ttCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTg                                                                                                                  <  2:1612064/150‑1 (MQ=255)

                                   gACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCa                                                                                                              >  2:4015243/1‑150 (MQ=255)

                                     cTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGc                                                                                                            >  1:2328773/1‑150 (MQ=255)

                                      tGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCa                                                                                                           >  2:2709692/1‑150 (MQ=255)

                                       gTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCaa                                                                                                          <  1:185227/150‑1 (MQ=255)

                                        tACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAAt                                                                                                         >  1:4815668/1‑150 (MQ=255)

                                          cGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCaaatc                                                                                                       >  1:256893/1‑147 (MQ=255)

                                          cGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCa                                                                                                       >  2:3740976/1‑150 (MQ=255)

                                           gCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCAc                                                                                                      >  2:3171047/1‑150 (MQ=255)

                                           gCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCAc                                                                                                      >  1:4387450/1‑150 (MQ=255)

                                           gCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCAc                                                                                                      >  2:3027134/1‑150 (MQ=255)

                                            ccAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACt                                                                                                     >  2:4859325/1‑150 (MQ=255)

                                            ccAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACt                                                                                                     <  2:3927495/150‑1 (MQ=255)

                                             cAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACtt                                                                                                    >  1:1046920/1‑150 (MQ=255)

                                             cAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACtt                                                                                                    >  1:2181135/1‑150 (MQ=255)

                                              aaTAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACttt                                                                                                   >  1:837763/1‑150 (MQ=255)

                                               aTAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTc                                                                                                  <  1:4746447/150‑1 (MQ=255)

                                                tAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCg                                                                                                 >  1:1496632/1‑150 (MQ=255)

                                                 aaCACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATACTTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCgg                                                                                                >  2:4185720/1‑150 (MQ=255)

                                                 aaCACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCgg                                                                                                <  1:3730483/150‑1 (MQ=255)

                                                       aaTGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGatcatc                                                                                          <  1:1273326/150‑1 (MQ=255)

                                                        aTGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCg                                                                                         <  1:200675/150‑1 (MQ=255)

                                                          ggTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTa                                                                                       >  2:404558/1‑150 (MQ=255)

                                                          ggTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTa                                                                                       <  1:3102937/150‑1 (MQ=255)

                                                            tCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAg                                                                                     >  2:1359467/1‑150 (MQ=255)

                                                            tCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAg                                                                                     >  2:2879225/1‑150 (MQ=255)

                                                            tCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAg                                                                                     >  2:186676/1‑150 (MQ=255)

                                                            tCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAg                                                                                     <  2:3859054/150‑1 (MQ=255)

                                                             cGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAgg                                                                                    >  1:3400118/1‑150 (MQ=255)

                                                                     tAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATaa                                                                            >  1:213209/1‑150 (MQ=255)

                                                                       gATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATaaaa                                                                          >  2:4615600/1‑150 (MQ=255)

                                                                          ttAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCCTCGTAAGGTGGGATAAAAATa                                                                       <  2:4698957/150‑1 (MQ=255)

                                                                           tAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATAc                                                                      >  2:4483791/1‑150 (MQ=255)

                                                                            aaTTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACg                                                                     >  2:3413925/1‑150 (MQ=255)

                                                                              ttGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGc                                                                   <  2:1719168/150‑1 (MQ=255)

                                                                               tGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGcc                                                                  <  2:2469076/150‑1 (MQ=255)

                                                                                 ccACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTNTCGGATCATCGTAAGGTGGGATAAAAATACGGCCtt                                                                <  1:2675958/150‑1 (MQ=255)

                                                                                  cACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTc                                                               <  2:1266106/150‑1 (MQ=255)

                                                                                  cACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGAACAATCGTTCCCTGGCCAGCACTCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTc                                                               >  1:5070562/1‑150 (MQ=255)

                                                                                   aCCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTcc                                                              <  1:3633874/150‑1 (MQ=255)

                                                                                   aCCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTcc                                                              <  1:4240381/150‑1 (MQ=255)

                                                                                     cGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCAt                                                            >  2:1426100/1‑150 (MQ=255)

                                                                                     cGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCAt                                                            >  2:606902/1‑150 (MQ=255)

                                                                                      gCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCAtt                                                           >  2:4352697/1‑150 (MQ=255)

                                                                                       cAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCAttt                                                          <  1:4689823/150‑1 (MQ=255)

                                                                                         aTACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCg                                                        >  2:4457676/1‑150 (MQ=255)

                                                                                          tACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGTATAAAAATACGGCCTTCCATTTCGa                                                       >  2:2626141/1‑150 (MQ=255)

                                                                                             cAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACaa                                                    >  1:4173478/1‑150 (MQ=255)

                                                                                              aGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAAt                                                   >  2:784051/1‑150 (MQ=255)

                                                                                                   ttAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGc                                              <  2:4499433/150‑1 (MQ=255)

                                                                                                     aaaCCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTc                                            <  2:1514084/150‑1 (MQ=255)

                                                                                                      aaCCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCa                                           <  2:218835/150‑1 (MQ=255)

                                                                                                        ccGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACt                                         <  2:4218372/150‑1 (MQ=255)

                                                                                                        ccGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACt                                         >  2:2116993/1‑150 (MQ=255)

                                                                                                          gCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCACTCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACttt                                       <  2:3251475/150‑1 (MQ=255)

                                                                                                          gCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACttt                                       >  2:738080/1‑150 (MQ=255)

                                                                                                          gCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACttt                                       >  2:4956021/1‑150 (MQ=255)

                                                                                                           ccaccaccaATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTa                                      <  1:3519542/150‑1 (MQ=255)

                                                                                                           ccaccaccaATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTa                                      >  1:304068/1‑150 (MQ=255)

                                                                                                           ccaccaccaATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTa                                      <  2:1042694/150‑1 (MQ=255)

                                                                                                           ccaccaccaATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGGTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTa                                      <  1:558225/150‑1 (MQ=255)

                                                                                                             accaccaATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGc                                    <  2:1039157/150‑1 (MQ=255)

                                                                                                               caccaATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGa                                  >  1:4216355/1‑150 (MQ=255)

                                                                                                                accaATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGAt                                 >  1:4651078/1‑150 (MQ=255)

                                                                                                                   aaTTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAgt                              <  1:490656/150‑1 (MQ=255)

                                                                                                                         cacaATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGtt                        <  1:5100933/150‑1 (MQ=255)

                                                                                                                         cacaATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGtt                        <  2:150935/150‑1 (MQ=255)

                                                                                                                         cacaATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGtt                        <  1:2709692/150‑1 (MQ=255)

                                                                                                                          acaATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGGTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTg                       <  2:174674/150‑1 (MQ=255)

                                                                                                                            aaTCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGaa                     >  1:2761236/1‑150 (MQ=255)

                                                                                                                            aaTCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGaa                     <  2:4966131/150‑1 (MQ=255)

                                                                                                                            aaTCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGaa                     >  1:1684552/1‑150 (MQ=255)

                                                                                                                            aaTCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATAAGGCCTTCCATTTCGACAATTTCGCTCACTTTAGAGATAGTGTCGTTGaa                     >  1:1988640/1‑150 (MQ=255)

                                                                                                                                aCGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTa                 <  1:4457676/150‑1 (MQ=255)

                                                                                                                                aCGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTa                 >  1:118228/1‑150 (MQ=255)

                                                                                                                                   ttATCGACATCATAGATATCTTCCATAATTTCCAGACCACTCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCa              <  1:3236246/150‑1 (MQ=255)

                                                                                                                                       cGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCAt          <  2:24766/150‑1 (MQ=255)

                                                                                                                                       cGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCAt          <  1:4961284/150‑1 (MQ=255)

                                                                                                                                       cGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCAt          >  2:3432864/1‑150 (MQ=255)

                                                                                                                                        gACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATg         <  2:4002214/150‑1 (MQ=255)

                                                                                                                                           atcatAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAg      <  2:4928338/150‑1 (MQ=255)

                                                                                                                                              atAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAAc   >  2:602370/1‑150 (MQ=255)

                                                                                                                                              atAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAAc   <  2:676138/150‑1 (MQ=255)

                                                                                                                                              atAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAAc   <  1:1359467/150‑1 (MQ=255)

AGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACG  >  CP009273/3258724‑3259016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACG  >  CP009273/3258722‑3259016

GGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGAGTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAT                                                                                                                                                   >  SRR19973063.2648143/1‑150 (MQ=60)

 GAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATA                                                                                                                                                  >  SRR19973063.1492354/1‑150 (MQ=60)

  AGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTTGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATAT                                                                                                                                                 >  SRR19973063.678114/1‑150 (MQ=60)

     GGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTT                                                                                                                                              >  SRR19973063.4944416/1‑150 (MQ=60)

     GGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTT                                                                                                                                              >  SRR19973063.4944257/1‑150 (MQ=60)

         AGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCAT                                                                                                                                          >  SRR19973063.2370558/1‑150 (MQ=60)

          GAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACAAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATA                                                                                                                                         <  SRR19973063.2407594/150‑1 (MQ=60)

            AGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCACTTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAAT                                                                                                                                       >  SRR19973063.24865/1‑150 (MQ=60)

            AGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAAT                                                                                                                                       >  SRR19973063.5038738/1‑150 (MQ=60)

              CCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTT                                                                                                                                     <  SRR19973063.3161590/150‑1 (MQ=60)

              CCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTT                                                                                                                                     <  SRR19973063.3161660/150‑1 (MQ=60)

                     GCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACC                                                                                                                              >  SRR19973063.791016/1‑150 (MQ=60)

                     GCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACC                                                                                                                              >  SRR19973063.1582668/1‑150 (MQ=60)

                     GCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACC                                                                                                                              >  SRR19973063.1582879/1‑150 (MQ=60)

                       CGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAA                                                                                                                            >  SRR19973063.2456575/1‑150 (MQ=60)

                        GTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAAT                                                                                                                           >  SRR19973063.4537873/1‑150 (MQ=60)

                        GTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAAT                                                                                                                           >  SRR19973063.3781386/1‑150 (MQ=60)

                           AACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGT                                                                                                                        >  SRR19973063.4877435/1‑150 (MQ=60)

                              GTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCC                                                                                                                     >  SRR19973063.1389216/1‑150 (MQ=60)

                              GTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCC                                                                                                                     <  SRR19973063.2148928/150‑1 (MQ=60)

                               TTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCC                                                                                                                    >  SRR19973063.4154621/1‑150 (MQ=60)

                                TTTCAGACTGTACGCAAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCT                                                                                                                   <  SRR19973063.2548849/150‑1 (MQ=60)

                                    AGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCC                                                                                                               <  SRR19973063.2335438/150‑1 (MQ=60)

                                      ACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAG                                                                                                             >  SRR19973063.4982469/1‑150 (MQ=60)

                                       CTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGC                                                                                                            >  SRR19973063.2363907/1‑150 (MQ=60)

                                       CTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGC                                                                                                            >  SRR19973063.2336457/1‑150 (MQ=60)

                                       CTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGC                                                                                                            >  SRR19973063.2336226/1‑150 (MQ=60)

                                         GTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAA                                                                                                          <  SRR19973063.185801/150‑1 (MQ=60)

                                          TACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAAT                                                                                                         >  SRR19973063.4831077/1‑150 (MQ=60)

                                            CGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAAatc                                                                                                       >  SRR19973063.257663/1‑147 (MQ=60)

                                            CGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCA                                                                                                       <  SRR19973063.2161306/150‑1 (MQ=60)

                                             GCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCAC                                                                                                      >  SRR19973063.4401621/1‑150 (MQ=60)

                                             GCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCAC                                                                                                      >  SRR19973063.4401502/1‑150 (MQ=60)

                                               CAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTT                                                                                                    >  SRR19973063.2187961/1‑150 (MQ=60)

                                               CAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTT                                                                                                    >  SRR19973063.1050091/1‑150 (MQ=60)

                                                AATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTT                                                                                                   >  SRR19973063.840183/1‑150 (MQ=60)

                                                 ATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTC                                                                                                  <  SRR19973063.1076128/150‑1 (MQ=60)

                                                 ATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTC                                                                                                  <  SRR19973063.4761514/150‑1 (MQ=60)

                                                 ATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTC                                                                                                  <  SRR19973063.4761560/150‑1 (MQ=60)

                                                  TAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCG                                                                                                 >  SRR19973063.1337561/1‑150 (MQ=60)

                                                  TAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCG                                                                                                 >  SRR19973063.1501216/1‑150 (MQ=60)

                                                   AACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGG                                                                                                >  SRR19973063.2476651/1‑150 (MQ=60)

                                                   AACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGG                                                                                                <  SRR19973063.3742398/150‑1 (MQ=60)

                                                        GAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCAT                                                                                           >  SRR19973063.3413409/1‑150 (MQ=60)

                                                         AATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATC                                                                                          <  SRR19973063.1277115/150‑1 (MQ=60)

                                                          ATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCG                                                                                         <  SRR19973063.201284/150‑1 (MQ=60)

                                                            GGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTA                                                                                       <  SRR19973063.3112920/150‑1 (MQ=60)

                                                              TCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAG                                                                                     <  SRR19973063.4865488/150‑1 (MQ=60)

                                                              TCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAG                                                                                     <  SRR19973063.56058/150‑1 (MQ=60)

                                                               CGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGG                                                                                    >  SRR19973063.3410937/1‑150 (MQ=60)

                                                                 GGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCgggccgatcggtccgtggccggcagtcaggggggggggggcgggagggg                                                                                  >  SRR19973063.4344787/1‑101 (MQ=60)

                                                                      ATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATA                                                                             <  SRR19973063.4576582/150‑1 (MQ=60)

                                                                       TAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAA                                                                            >  SRR19973063.213848/1‑150 (MQ=60)

                                                                            TTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATA                                                                       >  SRR19973063.4216244/1‑150 (MQ=60)

                                                                             TAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATAC                                                                      <  SRR19973063.3711387/150‑1 (MQ=60)

                                                                                TTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGC                                                                   <  SRR19973063.2607434/150‑1 (MQ=60)

                                                                                  GCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCT                                                                 <  SRR19973063.2742273/150‑1 (MQ=60)

                                                                                   CCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTNTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTT                                                                <  SRR19973063.2684561/150‑1 (MQ=60)

                                                                                    CACCGCAATACCAGCAATTAAACCGCCACCACCACTTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTC                                                               <  SRR19973063.5087794/150‑1 (MQ=60)

                                                                                    CACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTC                                                               <  SRR19973063.3041600/150‑1 (MQ=60)

                                                                                    CACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGAACAATCGTTCCCTGGCCAGCACTCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTC                                                               >  SRR19973063.5086872/1‑150 (MQ=60)

                                                                                     ACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCC                                                              <  SRR19973063.4253895/150‑1 (MQ=60)

                                                                                     ACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCC                                                              <  SRR19973063.4253610/150‑1 (MQ=60)

                                                                                     ACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCC                                                              <  SRR19973063.3645533/150‑1 (MQ=60)

                                                                                     ACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCC                                                              <  SRR19973063.3738582/150‑1 (MQ=60)

                                                                                        GCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATT                                                           >  SRR19973063.1507783/1‑150 (MQ=60)

                                                                                         CAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTT                                                          <  SRR19973063.4704771/150‑1 (MQ=60)

                                                                                           ATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCG                                                        <  SRR19973063.1250947/150‑1 (MQ=60)

                                                                                            TACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGA                                                       >  SRR19973063.4398897/1‑150 (MQ=60)

                                                                                              CCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACA                                                     <  SRR19973063.504034/150‑1 (MQ=60)

                                                                                              CCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACA                                                     >  SRR19973063.898609/1‑150 (MQ=60)

                                                                                              CCACCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCATGGACAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACA                                                     >  SRR19973063.2568587/1‑150 (MQ=60)

                                                                                               CAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAA                                                    >  SRR19973063.4186814/1‑150 (MQ=60)

                                                                                                 GCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATT                                                  <  SRR19973063.4316273/150‑1 (MQ=60)

                                                                                                   AATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTC                                                <  SRR19973063.5178182/150‑1 (MQ=60)

                                                                                                     TTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGC                                              <  SRR19973063.2635966/150‑1 (MQ=60)

                                                                                                     TTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGC                                              >  SRR19973063.1440404/1‑150 (MQ=60)

                                                                                                       AAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTC                                            <  SRR19973063.1979172/150‑1 (MQ=60)

                                                                                                       AAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTC                                            >  SRR19973063.2725868/1‑150 (MQ=60)

                                                                                                       AAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTC                                            <  SRR19973063.4874981/150‑1 (MQ=60)

                                                                                                        AACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCA                                           >  SRR19973063.1691799/1‑150 (MQ=60)

                                                                                                            GCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTT                                       >  SRR19973063.4745959/1‑150 (MQ=60)

                                                                                                             CCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTA                                      <  SRR19973063.3530802/150‑1 (MQ=60)

                                                                                                             CCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTA                                      >  SRR19973063.208667/1‑150 (MQ=60)

                                                                                                             CCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTA                                      >  SRR19973063.304962/1‑150 (MQ=60)

                                                                                                             CCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGGTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTA                                      <  SRR19973063.559876/150‑1 (MQ=60)

                                                                                                               ACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGC                                    <  SRR19973063.480679/150‑1 (MQ=60)

                                                                                                               ACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGC                                    <  SRR19973063.3605767/150‑1 (MQ=60)

                                                                                                                 CACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGA                                  <  SRR19973063.4230216/150‑1 (MQ=60)

                                                                                                                 CACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGA                                  >  SRR19973063.4229804/1‑150 (MQ=60)

                                                                                                                  ACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGAT                                 >  SRR19973063.4665932/1‑150 (MQ=60)

                                                                                                                  ACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGAT                                 >  SRR19973063.4665995/1‑150 (MQ=60)

                                                                                                                     AATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGT                              <  SRR19973063.492061/150‑1 (MQ=60)

                                                                                                                     AATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGT                              <  SRR19973063.1236658/150‑1 (MQ=60)

                                                                                                                      ATTGGCACAATCACGTTATCTACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTACCTGGCCAGCAATCAATTTCTGATCATAGTAATGTGGGATAAAAATACGGCCTTCCATTTCGAAAAATTCTCTAACTTAAGCGATAGTG                             >  SRR19973063.824339/1‑150 (MQ=60)

                                                                                                                        TGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTC                           <  SRR19973063.3110287/150‑1 (MQ=60)

                                                                                                                        TGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTC                           <  SRR19973063.161539/150‑1 (MQ=60)

                                                                                                                           CACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTT                        <  SRR19973063.2717842/150‑1 (MQ=60)

                                                                                                                           CACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTT                        <  SRR19973063.2718445/150‑1 (MQ=60)

                                                                                                                           CACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTT                        <  SRR19973063.5117349/150‑1 (MQ=60)

                                                                                                                              AATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAA                     >  SRR19973063.2770137/1‑150 (MQ=60)

                                                                                                                              AATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAA                     >  SRR19973063.1689682/1‑150 (MQ=60)

                                                                                                                              AATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAA                     >  SRR19973063.1994936/1‑150 (MQ=60)

                                                                                                                              AATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAA                     >  SRR19973063.1994747/1‑150 (MQ=60)

                                                                                                                              AATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATAAGGCCTTCCATTTCGACAATTTCGCTCACTTTAGAGATAGTGTCGTTGAA                     >  SRR19973063.1994858/1‑150 (MQ=60)

                                                                                                                                TCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGT                   >  SRR19973063.4182155/1‑150 (MQ=60)

                                                                                                                                  ACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTA                 >  SRR19973063.118620/1‑150 (MQ=60)

                                                                                                                                  ACGTTATCGACATCATAGATATCTTCCATAAGTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTA                 <  SRR19973063.4471984/150‑1 (MQ=60)

                                                                                                                                   CGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTAT                <  SRR19973063.5101930/150‑1 (MQ=60)

                                                                                                                                   CGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTAT                >  SRR19973063.3370918/1‑150 (MQ=60)

                                                                                                                                    GTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATC               <  SRR19973063.4881609/150‑1 (MQ=60)

                                                                                                                                     TTATCGACATCATAGATATCTTCCATAATTTCCAGACCACTCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCA              <  SRR19973063.3246643/150‑1 (MQ=60)

                                                                                                                                      TATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCAC             >  SRR19973063.2104299/1‑150 (MQ=60)

                                                                                                                                         CGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCAT          <  SRR19973063.4977321/150‑1 (MQ=60)

                                                                                                                                           ACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGC        <  SRR19973063.3489848/150‑1 (MQ=60)

                                                                                                                                              TCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGA     <  SRR19973063.4997847/150‑1 (MQ=60)

                                                                                                                                                ATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAAC   <  SRR19973063.1363553/150‑1 (MQ=60)

                                                                                                                                                 TAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACG  <  SRR19973063.3021586/150‑1 (MQ=60)

                                                                                                                                                 TAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACG  <  SRR19973063.3752926/150‑1 (MQ=60)

GGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACG  >  CP009273/3258722‑3259016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`