Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,258,871 G→T 85.1% L173I (CTC→ATC)  tdcB ← L‑threonine dehydratase, catabolic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,258,8710GT85.1% 638.2 / 122.3 279L173I (CTC→ATC) tdcBL‑threonine dehydratase, catabolic
Reads supporting (aligned to +/- strand):  ref base G (15/27);  new base T (123/114);  total (138/141)
Fisher's exact test for biased strand distribution p-value = 6.54e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

AGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACG  >  CP009273/3258724‑3259016
                                                                                                                                                   |                                                                                                                                                 
aGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAgaga                                                                                                                                                  <  1:224579/149‑1 (MQ=255)
     aaaGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTc                                                                                                                                             <  2:2755292/149‑1 (MQ=255)
       agaagCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGAGTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCa                                                                                                                                           <  2:1833565/149‑1 (MQ=255)
          agCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATaa                                                                                                                                        >  2:1526864/1‑149 (MQ=255)
          agCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATaa                                                                                                                                        >  1:395365/1‑149 (MQ=255)
           gccgccATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAAt                                                                                                                                       <  1:659260/149‑1 (MQ=255)
              gccATGCCGTGACCGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTc                                                                                                                                    >  2:4717555/1‑149 (MQ=255)
              gccATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTc                                                                                                                                    <  1:2427568/149‑1 (MQ=255)
              gccATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTc                                                                                                                                    <  1:575737/149‑1 (MQ=255)
               ccATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACTACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTcc                                                                                                                                   >  2:1518998/1‑149 (MQ=255)
               ccATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTcc                                                                                                                                   >  1:405456/1‑149 (MQ=255)
                 aTGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAg                                                                                                                                 >  2:417971/1‑149 (MQ=255)
                  tGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGa                                                                                                                                <  1:1710786/149‑1 (MQ=255)
                  tGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGa                                                                                                                                <  1:1802939/149‑1 (MQ=255)
                   gCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGAc                                                                                                                               <  2:2966621/149‑1 (MQ=255)
                    ccGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGAcc                                                                                                                              <  1:3015990/149‑1 (MQ=255)
                      gTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCaa                                                                                                                            <  1:2007905/149‑1 (MQ=255)
                       tGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAAt                                                                                                                           <  2:372343/149‑1 (MQ=255)
                          aCGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGt                                                                                                                        >  1:1121015/1‑149 (MQ=255)
                          aCGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGt                                                                                                                        <  1:4600414/149‑1 (MQ=255)
                            gTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATATATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTc                                                                                                                      <  1:2060745/149‑1 (MQ=255)
                              tttCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTccc                                                                                                                    <  2:1559980/149‑1 (MQ=255)
                              tttCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGGTccc                                                                                                                    <  2:3328045/149‑1 (MQ=255)
                               ttCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCt                                                                                                                   <  2:2387512/149‑1 (MQ=255)
                                   gACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGcc                                                                                                               <  1:949078/149‑1 (MQ=255)
                                   gACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGcc                                                                                                               <  1:1631966/149‑1 (MQ=255)
                                   gACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGcc                                                                                                               >  2:2973181/1‑149 (MQ=255)
                                        tACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCaa                                                                                                          >  1:1021637/1‑149 (MQ=255)
                                           gCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCa                                                                                                       <  2:584580/149‑1 (MQ=255)
                                           gCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGGTCCCTGGCCAGCAATCa                                                                                                       <  2:1602732/149‑1 (MQ=255)
                                            ccAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCAc                                                                                                      >  2:2431801/1‑149 (MQ=255)
                                             cAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACt                                                                                                     >  2:2208828/1‑149 (MQ=255)
                                              aaTAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACtt                                                                                                    <  1:3595501/149‑1 (MQ=255)
                                                tAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTc                                                                                                  >  1:1275349/1‑149 (MQ=255)
                                                     cGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGatc                                                                                             >  1:721910/1‑149 (MQ=255)
                                                     cGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGatc                                                                                             <  2:3183235/149‑1 (MQ=255)
                                                        aTGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGatcatc                                                                                          >  1:4142880/1‑149 (MQ=255)
                                                         tGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCACTCACTTTCGGATCATCg                                                                                         >  2:3434034/1‑149 (MQ=255)
                                                         tGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCg                                                                                         >  1:3997068/1‑149 (MQ=255)
                                                         tGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCg                                                                                         <  2:4406414/149‑1 (MQ=255)
                                                           gTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTa                                                                                       >  1:3907152/1‑149 (MQ=255)
                                                           gTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACAAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTa                                                                                       >  2:1644220/1‑149 (MQ=255)
                                                              gggTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAgg                                                                                    <  2:3391172/149‑1 (MQ=255)
                                                                gTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTg                                                                                  <  2:723515/149‑1 (MQ=255)
                                                                   aaTAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGa                                                                               >  1:3563265/1‑149 (MQ=255)
                                                                    aTAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGAt                                                                              <  2:3300639/149‑1 (MQ=255)
                                                                       gATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATaaa                                                                           >  2:1913211/1‑149 (MQ=255)
                                                                        aTTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATaaaa                                                                          >  2:1739780/1‑149 (MQ=255)
                                                                         tttAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATaaaaa                                                                         <  1:1425978/149‑1 (MQ=255)
                                                                            aaTTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATAc                                                                      >  2:2986518/1‑149 (MQ=255)
                                                                              ttGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACgg                                                                    >  2:3841032/1‑149 (MQ=255)
                                                                               tGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGc                                                                   >  2:4030419/1‑149 (MQ=255)
                                                                                gCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGcc                                                                  >  1:4750332/1‑149 (MQ=255)
                                                                                  cACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCtt                                                                <  1:4080471/149‑1 (MQ=255)
                                                                                  cACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCtt                                                                >  2:1483809/1‑149 (MQ=255)
                                                                                   aCCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCtgc                                                               >  2:4208958/1‑147 (MQ=255)
                                                                                     cGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCa                                                             <  2:3592120/149‑1 (MQ=255)
                                                                                      gCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCAt                                                            <  1:1943759/149‑1 (MQ=255)
                                                                                      gCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCAt                                                            <  2:2628578/149‑1 (MQ=255)
                                                                                       cAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCAtt                                                           <  2:453034/149‑1 (MQ=255)
                                                                                       cAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCAtt                                                           <  1:2115578/149‑1 (MQ=255)
                                                                                       cAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCAtt                                                           <  1:1751836/149‑1 (MQ=255)
                                                                                        aaTACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCAttt                                                          <  2:410079/149‑1 (MQ=255)
                                                                                        aaTACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCAttt                                                          >  2:2285536/1‑149 (MQ=255)
                                                                                         aTACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTc                                                         >  1:1041658/1‑149 (MQ=255)
                                                                                         aTACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTc                                                         >  1:4412876/1‑149 (MQ=255)
                                                                                            ccAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGAc                                                      >  1:2681050/1‑149 (MQ=255)
                                                                                            ccAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGAc                                                      >  1:4312941/1‑149 (MQ=255)
                                                                                              aGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACaa                                                    <  2:2869037/149‑1 (MQ=255)
                                                                                               gCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAAt                                                   >  2:2727052/1‑149 (MQ=255)
                                                                                                 aaTTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAAttt                                                 <  2:836078/149‑1 (MQ=255)
                                                                                                  aTTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTc                                                >  1:1137813/1‑149 (MQ=255)
                                                                                                  aTTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTc                                                <  2:1322698/149‑1 (MQ=255)
                                                                                                    aaaaCCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGc                                              <  2:3557674/148‑1 (MQ=255)
                                                                                                       aCCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCa                                           <  1:4153245/149‑1 (MQ=255)
                                                                                                          gCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACtt                                        <  1:249503/149‑1 (MQ=255)
                                                                                                           ccaccaccaATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACttt                                       <  1:647656/149‑1 (MQ=255)
                                                                                                            caccaccaATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTa                                      <  1:2433037/149‑1 (MQ=255)
                                                                                                              ccaccaATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGc                                    <  1:3077423/149‑1 (MQ=255)
                                                                                                                accaATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGa                                  >  2:3879590/1‑149 (MQ=255)
                                                                                                                 ccaATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGAt                                 >  2:971081/1‑149 (MQ=255)
                                                                                                                 ccaATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGAt                                 <  2:4164044/149‑1 (MQ=255)
                                                                                                                 ccaATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGAt                                 <  1:2523789/149‑1 (MQ=255)
                                                                                                                    aTTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAgt                              <  2:649539/149‑1 (MQ=255)
                                                                                                                     ttGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAgtg                             <  1:3308795/149‑1 (MQ=255)
                                                                                                                      tGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAgtgt                            >  1:1518185/1‑149 (MQ=255)
                                                                                                                            aaTCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGa                      >  2:2072146/1‑149 (MQ=255)
                                                                                                                             aTCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGaa                     <  1:827546/149‑1 (MQ=255)
                                                                                                                             aTCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGaa                     <  1:4110120/149‑1 (MQ=255)
                                                                                                                                aCGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGtt                  <  1:482301/149‑1 (MQ=255)
                                                                                                                                  gTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTAt                >  2:2303109/1‑149 (MQ=255)
                                                                                                                                   ttATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATc               <  2:2192295/149‑1 (MQ=255)
                                                                                                                                   ttATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATc               >  2:3909574/1‑149 (MQ=255)
                                                                                                                                   ttATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATc               >  1:3747416/1‑149 (MQ=255)
                                                                                                                                    tATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCa              >  1:3444589/1‑149 (MQ=255)
                                                                                                                                     aTCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCAc             >  2:1796027/1‑149 (MQ=255)
                                                                                                                                          catcatAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGc        >  1:1479035/1‑149 (MQ=255)
                                                                                                                                             catAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGa     <  2:4746986/149‑1 (MQ=255)
                                                                                                                                               tAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAAc   >  1:3184568/1‑149 (MQ=255)
                                                                                                                                                agagATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACg  >  2:210793/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACG  >  CP009273/3258724‑3259016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTT  >  CP009273/3258722‑3259020
                                                                                                                                                     |                                                                                                                                                     
GGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAT                                                                                                                                                       <  SRR19973071.4462080/150‑1 (MQ=60)
  AGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGAT                                                                                                                                                     <  SRR19973071.225456/150‑1 (MQ=60)
       AAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCC                                                                                                                                                <  SRR19973071.1976814/150‑1 (MQ=60)
       AAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCC                                                                                                                                                <  SRR19973071.3158078/150‑1 (MQ=60)
          GAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATA                                                                                                                                             <  SRR19973071.435716/150‑1 (MQ=60)
           AAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAA                                                                                                                                            >  SRR19973071.2081812/1‑150 (MQ=60)
           AAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAA                                                                                                                                            >  SRR19973071.396591/1‑150 (MQ=60)
             GCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATT                                                                                                                                          <  SRR19973071.691117/150‑1 (MQ=60)
             GCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATT                                                                                                                                          <  SRR19973071.661651/150‑1 (MQ=60)
              CCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTT                                                                                                                                         >  SRR19973071.1029759/1‑150 (MQ=60)
                GCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCC                                                                                                                                       <  SRR19973071.2436350/150‑1 (MQ=60)
                GCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCC                                                                                                                                       >  SRR19973071.406729/1‑150 (MQ=60)
                GCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCC                                                                                                                                       >  SRR19973071.406751/1‑150 (MQ=60)
                GCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCC                                                                                                                                       >  SRR19973071.406808/1‑150 (MQ=60)
                GCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCC                                                                                                                                       <  SRR19973071.1516591/150‑1 (MQ=60)
                GCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCC                                                                                                                                       <  SRR19973071.577897/150‑1 (MQ=60)
                 CCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCA                                                                                                                                      >  SRR19973071.2342851/1‑150 (MQ=60)
                  CATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAG                                                                                                                                     <  SRR19973071.3270544/150‑1 (MQ=60)
                  CATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAG                                                                                                                                     >  SRR19973071.2509960/1‑150 (MQ=60)
                    TGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGAC                                                                                                                                   <  SRR19973071.1809652/150‑1 (MQ=60)
                    TGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGAC                                                                                                                                   <  SRR19973071.1717137/150‑1 (MQ=60)
                      CCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCA                                                                                                                                 <  SRR19973071.3026748/150‑1 (MQ=60)
                       CGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAA                                                                                                                                >  SRR19973071.3066126/1‑150 (MQ=60)
                       CGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAA                                                                                                                                >  SRR19973071.3065609/1‑150 (MQ=60)
                        GTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAAT                                                                                                                               <  SRR19973071.2015160/150‑1 (MQ=60)
                           AACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGT                                                                                                                            >  SRR19973071.1125564/1‑150 (MQ=60)
                           AACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGT                                                                                                                            >  SRR19973071.1124954/1‑150 (MQ=60)
                            ACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTT                                                                                                                           <  SRR19973071.4616819/150‑1 (MQ=60)
                             CGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTC                                                                                                                          <  SRR19973071.3265579/150‑1 (MQ=60)
                              GTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATATATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCC                                                                                                                         <  SRR19973071.2068319/150‑1 (MQ=60)
                               TTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCC                                                                                                                        <  SRR19973071.4729110/150‑1 (MQ=60)
                                TTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCT                                                                                                                       <  SRR19973071.1793862/150‑1 (MQ=60)
                                 TTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTG                                                                                                                      <  SRR19973071.1237416/150‑1 (MQ=60)
                                  TCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGG                                                                                                                     >  SRR19973071.113201/1‑150 (MQ=60)
                                  TCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGG                                                                                                                     >  SRR19973071.2513882/1‑150 (MQ=60)
                                  TCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGG                                                                                                                     <  SRR19973071.4716769/150‑1 (MQ=60)
                                     GACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCA                                                                                                                  <  SRR19973071.952521/150‑1 (MQ=60)
                                     GACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCA                                                                                                                  <  SRR19973071.1637855/150‑1 (MQ=60)
                                     GACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCA                                                                                                                  <  SRR19973071.1638003/150‑1 (MQ=60)
                                       CTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGC                                                                                                                >  SRR19973071.3897063/1‑150 (MQ=60)
                                       CTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGC                                                                                                                >  SRR19973071.1447256/1‑150 (MQ=60)
                                         GTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAA                                                                                                              >  SRR19973071.1025337/1‑150 (MQ=60)
                                            CGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCA                                                                                                           >  SRR19973071.3604586/1‑150 (MQ=60)
                                            CGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCA                                                                                                           >  SRR19973071.3604590/1‑150 (MQ=60)
                                                AATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTT                                                                                                       <  SRR19973071.4614812/150‑1 (MQ=60)
                                                AATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTT                                                                                                       <  SRR19973071.3607994/150‑1 (MQ=60)
                                                 ATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTC                                                                                                      >  SRR19973071.1279971/1‑150 (MQ=60)
                                                     CACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGAT                                                                                                  <  SRR19973071.1734904/150‑1 (MQ=60)
                                                      ACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATC                                                                                                 >  SRR19973071.724419/1‑150 (MQ=60)
                                                      ACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATC                                                                                                 >  SRR19973071.724390/1‑150 (MQ=60)
                                                         AATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCTTTCCCTGGCCAGCAATCACTTTCGGATCATC                                                                                              >  SRR19973071.3340102/1‑150 (MQ=60)
                                                         AATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATC                                                                                              >  SRR19973071.4157631/1‑150 (MQ=60)
                                                         AATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATC                                                                                              >  SRR19973071.4158083/1‑150 (MQ=60)
                                                         AATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATC                                                                                              <  SRR19973071.1423107/150‑1 (MQ=60)
                                                          ATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCG                                                                                             >  SRR19973071.4011045/1‑150 (MQ=60)
                                                            GGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTA                                                                                           >  SRR19973071.3920938/1‑150 (MQ=60)
                                                            GGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTA                                                                                           <  SRR19973071.1993260/150‑1 (MQ=60)
                                                               CGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGG                                                                                        >  SRR19973071.4192527/1‑150 (MQ=60)
                                                                    TAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGA                                                                                   >  SRR19973071.3575678/1‑150 (MQ=60)
                                                                      ATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATA                                                                                 <  SRR19973071.835004/150‑1 (MQ=60)
                                                                      ATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTGTCGGATCATCGTAAGGTGGGATA                                                                                 >  SRR19973071.4135649/1‑150 (MQ=60)
                                                                         GATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAA                                                                              >  SRR19973071.3998666/1‑150 (MQ=60)
                                                                           TTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAAT                                                                            <  SRR19973071.1431220/150‑1 (MQ=60)
                                                                                 TGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCC                                                                      <  SRR19973071.246436/150‑1 (MQ=60)
                                                                                 TGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCC                                                                      >  SRR19973071.4767178/1‑150 (MQ=60)
                                                                                 TGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCC                                                                      >  SRR19973071.4767138/1‑150 (MQ=60)
                                                                                 TGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCC                                                                      <  SRR19973071.245912/150‑1 (MQ=60)
                                                                                   CCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTT                                                                    >  SRR19973071.2913022/1‑150 (MQ=60)
                                                                                   CCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTT                                                                    >  SRR19973071.432998/1‑150 (MQ=60)
                                                                                   CCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTT                                                                    <  SRR19973071.2018689/150‑1 (MQ=60)
                                                                                   CCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTT                                                                    <  SRR19973071.2017960/150‑1 (MQ=60)
                                                                                   CCACAGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTT                                                                    <  SRR19973071.411404/150‑1 (MQ=60)
                                                                                    CACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTC                                                                   <  SRR19973071.4094842/150‑1 (MQ=60)
                                                                                    CACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTC                                                                   <  SRR19973071.4094949/150‑1 (MQ=60)
                                                                                        GCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATT                                                               <  SRR19973071.1950835/150‑1 (MQ=60)
                                                                                         CAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTT                                                              <  SRR19973071.2123390/150‑1 (MQ=60)
                                                                                         CAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTT                                                              <  SRR19973071.1758379/150‑1 (MQ=60)
                                                                                         CAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTT                                                              >  SRR19973071.2155672/1‑150 (MQ=60)
                                                                                         CAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTT                                                              >  SRR19973071.2155703/1‑150 (MQ=60)
                                                                                         CAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACGTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTT                                                              <  SRR19973071.1758493/150‑1 (MQ=60)
                                                                                          AATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTC                                                             >  SRR19973071.1045410/1‑150 (MQ=60)
                                                                                          AATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTC                                                             >  SRR19973071.4428410/1‑150 (MQ=60)
                                                                                          AATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTC                                                             >  SRR19973071.1045414/1‑150 (MQ=60)
                                                                                             ACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGAC                                                          >  SRR19973071.4328186/1‑150 (MQ=60)
                                                                                             ACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGAC                                                          >  SRR19973071.2690639/1‑150 (MQ=60)
                                                                                             ACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGAC                                                          >  SRR19973071.2690495/1‑150 (MQ=60)
                                                                                             ACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGAC                                                          >  SRR19973071.2690457/1‑150 (MQ=60)
                                                                                             ACCAGCAATTAAACCGCCACCACCAATAGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGAC                                                          >  SRR19973071.3986859/1‑150 (MQ=60)
                                                                                                 GCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATT                                                      >  SRR19973071.1685270/1‑150 (MQ=60)
                                                                                                   AATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTC                                                    >  SRR19973071.1141790/1‑150 (MQ=60)
                                                                                                    ATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCG                                                   >  SRR19973071.1097631/1‑150 (MQ=60)
                                                                                                    ATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCG                                                   <  SRR19973071.2440649/150‑1 (MQ=60)
                                                                                                      TAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCT                                                 <  SRR19973071.36503/150‑1 (MQ=60)
                                                                                                      TAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCT                                                 <  SRR19973071.36542/150‑1 (MQ=60)
                                                                                                       AAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTC                                                <  SRR19973071.1566837/150‑1 (MQ=60)
                                                                                                        AACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCA                                               <  SRR19973071.1916516/150‑1 (MQ=60)
                                                                                                         ACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCAC                                              <  SRR19973071.4168029/150‑1 (MQ=60)
                                                                                                         ACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCAC                                              <  SRR19973071.4168034/150‑1 (MQ=60)
                                                                                                         ACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCAC                                              >  SRR19973071.349193/1‑150 (MQ=60)
                                                                                                         ACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCAC                                              >  SRR19973071.349223/1‑150 (MQ=60)
                                                                                                          CCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTCCGACAATTTCGCTCACT                                             >  SRR19973071.652652/1‑150 (MQ=60)
                                                                                                            GCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTT                                           <  SRR19973071.250448/150‑1 (MQ=60)
                                                                                                            GCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTT                                           >  SRR19973071.3936869/1‑150 (MQ=60)
                                                                                                            GCCACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTT                                           >  SRR19973071.3936710/1‑150 (MQ=60)
                                                                                                            GCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTT                                           <  SRR19973071.2122020/150‑1 (MQ=60)
                                                                                                             CCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTA                                          <  SRR19973071.650027/150‑1 (MQ=60)
                                                                                                              CACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAG                                         <  SRR19973071.2441864/150‑1 (MQ=60)
                                                                                                              CACCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAG                                         <  SRR19973071.4190282/150‑1 (MQ=60)
                                                                                                                CCACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCG                                       <  SRR19973071.3088318/150‑1 (MQ=60)
                                                                                                                 CACCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGA                                      <  SRR19973071.1621686/150‑1 (MQ=60)
                                                                                                                   CCAATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATA                                    <  SRR19973071.2532972/150‑1 (MQ=60)
                                                                                                                     AATTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGT                                  >  SRR19973071.1161697/1‑150 (MQ=60)
                                                                                                                       TTGGCACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGT                                >  SRR19973071.1523663/1‑150 (MQ=60)
                                                                                                                       TTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGT                                <  SRR19973071.3320623/150‑1 (MQ=60)
                                                                                                                       TTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGT                                <  SRR19973071.3320524/150‑1 (MQ=60)
                                                                                                                       TTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGT                                <  SRR19973071.2303437/150‑1 (MQ=60)
                                                                                                                        TGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTC                               >  SRR19973071.1503196/1‑150 (MQ=60)
                                                                                                                            ACAATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTG                           <  SRR19973071.4037468/150‑1 (MQ=60)
                                                                                                                               ATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAG                        <  SRR19973071.4124589/150‑1 (MQ=60)
                                                                                                                               ATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAG                        <  SRR19973071.830417/150‑1 (MQ=60)
                                                                                                                               ATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAG                        <  SRR19973071.830210/150‑1 (MQ=60)
                                                                                                                               ATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAG                        <  SRR19973071.1756387/150‑1 (MQ=60)
                                                                                                                               ATCACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAG                        <  SRR19973071.4124742/150‑1 (MQ=60)
                                                                                                                               ATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAG                        <  SRR19973071.2783911/150‑1 (MQ=60)
                                                                                                                                TCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGT                       <  SRR19973071.654705/150‑1 (MQ=60)
                                                                                                                                  ACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTA                     <  SRR19973071.484077/150‑1 (MQ=60)
                                                                                                                                  ACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTA                     <  SRR19973071.484396/150‑1 (MQ=60)
                                                                                                                                  ACGTTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTA                     <  SRR19973071.1291466/150‑1 (MQ=60)
                                                                                                                                  ACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTA                     <  SRR19973071.484087/150‑1 (MQ=60)
                                                                                                                                    GTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATC                   >  SRR19973071.3760710/1‑150 (MQ=60)
                                                                                                                                     TTATCGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCA                  >  SRR19973071.3456753/1‑150 (MQ=60)
                                                                                                                                         CGACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCAT              <  SRR19973071.76313/150‑1 (MQ=60)
                                                                                                                                           ACATCATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGC            >  SRR19973071.1484418/1‑150 (MQ=60)
                                                                                                                                                ATAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAAC       >  SRR19973071.3195651/1‑150 (MQ=60)
                                                                                                                                                 TAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACG      <  SRR19973071.63541/150‑1 (MQ=60)
                                                                                                                                                 TAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACG      <  SRR19973071.63443/150‑1 (MQ=60)
                                                                                                                                                 TAGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATAACCAAGCAGAACG      >  SRR19973071.4456956/1‑150 (MQ=60)
                                                                                                                                                  AGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGA     >  SRR19973071.2755152/1‑150 (MQ=60)
                                                                                                                                                  AGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGA     >  SRR19973071.4622845/1‑150 (MQ=60)
                                                                                                                                                  AGATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAAAGA     >  SRR19973071.4622794/1‑150 (MQ=60)
                                                                                                                                                   GATATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGAC    <  SRR19973071.3620617/150‑1 (MQ=60)
                                                                                                                                                     TATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTT  <  SRR19973071.4514370/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTT  >  CP009273/3258722‑3259020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑