Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,024,983 C→G 94.4% G86G (GGC→GGG)  uacT → uric acid permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,024,9830CG94.4% 949.6 / 41.5 250G86G (GGC→GGG) uacTuric acid permease
Reads supporting (aligned to +/- strand):  ref base C (5/9);  new base G (128/108);  total (133/117)
Fisher's exact test for biased strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 251 total aligned reads displayed.

GTCCTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACATTATTA  >  CP009273/3024837‑3025130
                                                                                                                                                  |                                                                                                                                                   
gTCCTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTAACATTATTGAAATGTATCGGTATCGGgcgc                                                                                                                                                  >  2:1287527/1‑150 (MQ=255)
   cTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCttt                                                                                                                                               <  1:3705101/150‑1 (MQ=255)
     tGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTAt                                                                                                                                             <  2:2211664/150‑1 (MQ=255)
      gTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATg                                                                                                                                            >  2:2941070/1‑150 (MQ=255)
      gTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCGTTATg                                                                                                                                            >  1:688445/1‑150 (MQ=255)
       tCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATgg                                                                                                                                           <  2:4265360/150‑1 (MQ=255)
       tCATGTACGCAGGTGCAGTCGCTGTTCCACTTATGATTTGTGACCGAATGGGCCTCTCAAAAGAATCTATTGCGATGCTCATTAGCTCGGATCTCTTTTTCTGCGGGATCGTCACCTTATTGCAATGTATCGGTATCGGGCGCGTGATgg                                                                                                                                           >  1:3217346/1‑150 (MQ=255)
          tGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGa                                                                                                                                        <  1:446002/150‑1 (MQ=255)
          tGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGa                                                                                                                                        <  1:1613464/150‑1 (MQ=255)
            tACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATc                                                                                                                                      <  2:2420592/150‑1 (MQ=255)
                cAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGcc                                                                                                                                  <  2:1598452/150‑1 (MQ=255)
                 aGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCt                                                                                                                                 <  2:2830982/150‑1 (MQ=255)
                 aGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTAATGCGATGCGCATTAGCTCGGATCTCGGTTGCTGCGGGATCGTCACAGTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATcctcct                                                                                                                                 >  2:761086/1‑150 (MQ=255)
                  ggTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTg                                                                                                                                <  2:1231930/150‑1 (MQ=255)
                     gCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCg                                                                                                                             <  2:1352487/150‑1 (MQ=255)
                     gCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTAGTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCg                                                                                                                             <  2:238507/150‑1 (MQ=255)
                        gTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTg                                                                                                                          <  2:514777/150‑1 (MQ=255)
                           gCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGAtt                                                                                                                       <  1:1413771/150‑1 (MQ=255)
                              gTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATg                                                                                                                    >  2:3668516/1‑150 (MQ=255)
                                tCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTc                                                                                                                  <  1:877883/150‑1 (MQ=255)
                                tCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTc                                                                                                                  <  1:1401956/150‑1 (MQ=255)
                                   tctTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGt                                                                                                               >  2:1966071/1‑150 (MQ=255)
                                    ctTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTTCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTg                                                                                                              <  2:1200714/150‑1 (MQ=255)
                                    ctTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTg                                                                                                              <  2:3180645/150‑1 (MQ=255)
                                     ttATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGa                                                                                                             <  2:2651266/150‑1 (MQ=255)
                                     ttATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGa                                                                                                             >  2:869363/1‑150 (MQ=255)
                                      tATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGAc                                                                                                            >  2:3161884/1‑150 (MQ=255)
                                       aTGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGAcc                                                                                                           >  2:2997668/1‑150 (MQ=255)
                                          aTTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCttt                                                                                                        <  2:2563029/150‑1 (MQ=255)
                                          aTTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCttt                                                                                                        <  1:3220117/150‑1 (MQ=255)
                                          aTTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCttt                                                                                                        <  1:2058625/150‑1 (MQ=255)
                                            tGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTtgc                                                                                                      >  1:3776208/1‑150 (MQ=255)
                                             ggTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTtgct                                                                                                     <  2:942712/150‑1 (MQ=255)
                                                gACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTgctgct                                                                                                  <  2:1158091/150‑1 (MQ=255)
                                                gACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTgctgct                                                                                                  <  1:1725256/150‑1 (MQ=255)
                                                gACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTgctgct                                                                                                  <  1:1009239/150‑1 (MQ=255)
                                                   cGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTa                                                                                               <  1:3818350/150‑1 (MQ=255)
                                                   cGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTa                                                                                               >  2:102256/1‑150 (MQ=255)
                                                    gACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTaa                                                                                              >  1:2721151/1‑150 (MQ=255)
                                                      cTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAaca                                                                                            >  1:4085435/1‑150 (MQ=255)
                                                           ccTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAAt                                                                                       >  1:3888667/1‑150 (MQ=255)
                                                             tctcAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGa                                                                                     <  2:2061974/150‑1 (MQ=255)
                                                              ctcAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGAt                                                                                    >  1:4423009/1‑150 (MQ=255)
                                                               tcAAAAGAAGCTATTGCGATGCTCATTAGCTAGGATCTCTGTTGCTGCGGGATCGTCAAATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCAGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATa                                                                                   >  2:484806/1‑150 (MQ=255)
                                                                  aaaGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGcc                                                                                <  1:3825252/150‑1 (MQ=255)
                                                                  aaaGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGcc                                                                                >  2:262627/1‑150 (MQ=255)
                                                                   aagaagCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCa                                                                               >  2:4492019/1‑150 (MQ=255)
                                                                    agaagCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCAt                                                                              >  2:1845427/1‑150 (MQ=255)
                                                                    agaagCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCAt                                                                              <  2:1731186/150‑1 (MQ=255)
                                                                    agaagCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCAt                                                                              >  1:2959881/1‑150 (MQ=255)
                                                                     gaagCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACAGTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCAtt                                                                             >  1:2896139/1‑150 (MQ=255)
                                                                      aagCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTg                                                                            >  2:366830/1‑150 (MQ=255)
                                                                      aagCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTg                                                                            >  1:3442997/1‑150 (MQ=255)
                                                                       agCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTgg                                                                           >  1:3205416/1‑150 (MQ=255)
                                                                        gCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTggg                                                                          <  2:392921/150‑1 (MQ=255)
                                                                                gATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAAccc                                                                  >  2:20378/1‑150 (MQ=255)
                                                                                      cATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGatat                                                            >  2:1703932/1‑150 (MQ=255)
                                                                                       aTTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTCTTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATc                                                           >  2:2651804/1‑150 (MQ=255)
                                                                                       aTTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATc                                                           >  2:2762321/1‑150 (MQ=255)
                                                                                       aTTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATc                                                           >  1:2223507/1‑150 (MQ=255)
                                                                                       aTTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATc                                                           <  2:587951/150‑1 (MQ=255)
                                                                                       aTTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATc                                                           <  1:407084/150‑1 (MQ=255)
                                                                                        ttAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCg                                                          <  2:829142/150‑1 (MQ=255)
                                                                                         tAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCgg                                                         >  1:1690967/1‑150 (MQ=255)
                                                                                         tAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCgg                                                         >  1:4016010/1‑150 (MQ=255)
                                                                                          aGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGc                                                        >  2:4161865/1‑150 (MQ=255)
                                                                                            cTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCct                                                      <  1:4333895/150‑1 (MQ=255)
                                                                                              cGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCctgc                                                    >  1:3590513/1‑150 (MQ=255)
                                                                                               ggATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCctgct                                                   <  1:1927909/150‑1 (MQ=255)
                                                                                               ggATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCctgct                                                   <  2:1014777/150‑1 (MQ=255)
                                                                                               ggATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCctgct                                                   >  1:3910930/1‑150 (MQ=255)
                                                                                                 aTCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCATTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTgg                                                 >  2:832185/1‑150 (MQ=255)
                                                                                                    tctTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGa                                              >  2:1990599/1‑150 (MQ=255)
                                                                                                      ttttGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGata                                            <  1:4274385/150‑1 (MQ=255)
                                                                                                      ttttGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGAGATTATGTCGGTGACCTTTGCAGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGata                                            >  1:3472870/1‑150 (MQ=255)
                                                                                                        ttGCTGCGGGATCGTCACATTATTGCAATGTATAGGTATCGGGAGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATAtt                                          <  1:588388/150‑1 (MQ=255)
                                                                                                          gctgcGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTg                                        <  2:420734/150‑1 (MQ=255)
                                                                                                          gctgcGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTg                                        <  2:2195504/150‑1 (MQ=255)
                                                                                                            tgcGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGt                                      >  1:1033500/1‑150 (MQ=255)
                                                                                                            tgcGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGt                                      >  1:3090256/1‑150 (MQ=255)
                                                                                                            tgcGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGt                                      <  2:4453046/150‑1 (MQ=255)
                                                                                                               gggATCGTCACATTCTTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGcc                                   >  1:1264823/1‑150 (MQ=255)
                                                                                                               gggATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGcc                                   >  1:3023503/1‑150 (MQ=255)
                                                                                                                ggATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCa                                  <  1:2335545/150‑1 (MQ=255)
                                                                                                                   tCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGACGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGAAATCGGCCTGCTGGGGATATTTGGTGCCACTa                               >  1:762919/1‑150 (MQ=255)
                                                                                                                     gTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATc                             <  1:1106628/150‑1 (MQ=255)
                                                                                                                      tCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATcg                            <  2:4424099/150‑1 (MQ=255)
                                                                                                                       cacaTTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATcgc                           <  1:2581457/150‑1 (MQ=255)
                                                                                                                        acaTTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATcgcc                          >  1:364892/1‑150 (MQ=255)
                                                                                                                          attattGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATcgccgc                        >  2:2717626/1‑150 (MQ=255)
                                                                                                                          attattGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATcgccgc                        >  2:4588822/1‑150 (MQ=255)
                                                                                                                                 cAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGtttt                 >  2:1906741/1‑150 (MQ=255)
                                                                                                                                   aTGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTAt               <  1:2475592/150‑1 (MQ=255)
                                                                                                                                     gtatcggtatcgGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATca             <  1:857059/150‑1 (MQ=255)
                                                                                                                                      tatcggtatcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATcac            >  1:4550492/1‑150 (MQ=255)
                                                                                                                                         cggtatcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATcaccac         >  2:1806564/1‑150 (MQ=255)
                                                                                                                                          ggtatcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACcaca        >  2:3052616/1‑150 (MQ=255)
                                                                                                                                             atcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACatta     <  2:388039/150‑1 (MQ=255)
                                                                                                                                             atcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACatta     <  1:162137/150‑1 (MQ=255)
                                                                                                                                              tcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACattat    <  1:3596637/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GTCCTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACATTATTA  >  CP009273/3024837‑3025130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACATTATTAGC  >  CP009273/3024840‑3025132
                                                                                                                                               |                                                                                                                                                     
CTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTT                                                                                                                                                 <  SRR19973073.3712544/150‑1 (MQ=60)
CTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTT                                                                                                                                                 <  SRR19973073.2417458/150‑1 (MQ=60)
   GTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCGTTATG                                                                                                                                              >  SRR19973073.689879/1‑150 (MQ=60)
    TCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTTGTGACCGACTGGGCCTCTCAAAAGAAACTAATGCGATTCTCATTAGCTAGGATGTCTTTTGCTGCGGGATCGTCACATTATTGCAAAGTATCGGTATCGGGCGCTTTATGG                                                                                                                                             >  SRR19973073.3223824/1‑150 (MQ=60)
    TCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGG                                                                                                                                             <  SRR19973073.2949763/150‑1 (MQ=60)
    TCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGG                                                                                                                                             <  SRR19973073.2949765/150‑1 (MQ=60)
    TCATGTACGCAGGTGCAGTCGCTGTTCCACTTATGATTTGTGACCGAATGGGCCTCTCAAAAGAATCTATTGCGATGCTCATTAGCTCGGATCTCTTTTTCTGCGGGATCGTCACCTTATTGCAATGTATCGGTATCGGGCGCGTGATGG                                                                                                                                             >  SRR19973073.3223836/1‑150 (MQ=60)
       TGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGA                                                                                                                                          <  SRR19973073.1616797/150‑1 (MQ=60)
       TGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGA                                                                                                                                          <  SRR19973073.1616791/150‑1 (MQ=60)
       TGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGA                                                                                                                                          <  SRR19973073.446918/150‑1 (MQ=60)
                        GCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATT                                                                                                                         <  SRR19973073.1416753/150‑1 (MQ=60)
                          TGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTAT                                                                                                                       <  SRR19973073.1830348/150‑1 (MQ=60)
                             TCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTC                                                                                                                    <  SRR19973073.1404920/150‑1 (MQ=60)
                             TCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTC                                                                                                                    <  SRR19973073.879693/150‑1 (MQ=60)
                             TCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTC                                                                                                                    <  SRR19973073.3840438/150‑1 (MQ=60)
                             TCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTC                                                                                                                    <  SRR19973073.1405061/150‑1 (MQ=60)
                                  TTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGA                                                                                                               <  SRR19973073.262538/150‑1 (MQ=60)
                                       ATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTT                                                                                                          <  SRR19973073.3740756/150‑1 (MQ=60)
                                       ATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTT                                                                                                          <  SRR19973073.3226612/150‑1 (MQ=60)
                                       ATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTT                                                                                                          <  SRR19973073.2062910/150‑1 (MQ=60)
                                        TTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTG                                                                                                         >  SRR19973073.4356422/1‑150 (MQ=60)
                                         TGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGC                                                                                                        >  SRR19973073.3783770/1‑150 (MQ=60)
                                         TGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGC                                                                                                        >  SRR19973073.3783807/1‑150 (MQ=60)
                                          GGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCT                                                                                                       <  SRR19973073.695903/150‑1 (MQ=60)
                                             GACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCT                                                                                                    >  SRR19973073.91236/1‑150 (MQ=60)
                                             GACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCT                                                                                                    <  SRR19973073.1011425/150‑1 (MQ=60)
                                             GACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCT                                                                                                    <  SRR19973073.1728860/150‑1 (MQ=60)
                                             GACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCT                                                                                                    <  SRR19973073.1728838/150‑1 (MQ=60)
                                             GACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCT                                                                                                    <  SRR19973073.1011349/150‑1 (MQ=60)
                                              ACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTG                                                                                                   >  SRR19973073.4460607/1‑150 (MQ=60)
                                              ACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTG                                                                                                   >  SRR19973073.1588611/1‑150 (MQ=60)
                                               CCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGGTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGT                                                                                                  >  SRR19973073.3337931/1‑150 (MQ=60)
                                                CGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTA                                                                                                 <  SRR19973073.3826432/150‑1 (MQ=60)
                                                CGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTA                                                                                                 <  SRR19973073.3825956/150‑1 (MQ=60)
                                                CGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTA                                                                                                 <  SRR19973073.3826161/150‑1 (MQ=60)
                                                CGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTA                                                                                                 >  SRR19973073.3702434/1‑150 (MQ=60)
                                                 GACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAA                                                                                                >  SRR19973073.2726711/1‑150 (MQ=60)
                                                  ACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAAC                                                                                               >  SRR19973073.3653527/1‑150 (MQ=60)
                                                   CTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACA                                                                                              >  SRR19973073.4093606/1‑150 (MQ=60)
                                                   CTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACA                                                                                              >  SRR19973073.4093616/1‑150 (MQ=60)
                                                     GGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACC                                                                                            >  SRR19973073.1399751/1‑150 (MQ=60)
                                                        CCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAAT                                                                                         >  SRR19973073.3896384/1‑150 (MQ=60)
                                                          TCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGA                                                                                       <  SRR19973073.2482301/150‑1 (MQ=60)
                                                           CTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGAT                                                                                      >  SRR19973073.4431888/1‑150 (MQ=60)
                                                            TCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATA                                                                                     <  SRR19973073.2245460/150‑1 (MQ=60)
                                                               AAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCC                                                                                  <  SRR19973073.3832861/150‑1 (MQ=60)
                                                                 AGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCAT                                                                                >  SRR19973073.2965912/1‑150 (MQ=60)
                                                                  GAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACAGTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATT                                                                               >  SRR19973073.2902051/1‑150 (MQ=60)
                                                                   AAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTG                                                                              >  SRR19973073.3449864/1‑150 (MQ=60)
                                                                    AGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGG                                                                             >  SRR19973073.3211894/1‑150 (MQ=60)
                                                                    AGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGG                                                                             >  SRR19973073.3211982/1‑150 (MQ=60)
                                                                      CTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGA                                                                           >  SRR19973073.873015/1‑150 (MQ=60)
                                                                        ATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATG                                                                         >  SRR19973073.672794/1‑150 (MQ=60)
                                                                        ATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATG                                                                         >  SRR19973073.673425/1‑150 (MQ=60)
                                                                        ATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATG                                                                         >  SRR19973073.259599/1‑150 (MQ=60)
                                                                        ATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATG                                                                         <  SRR19973073.3929212/150‑1 (MQ=60)
                                                                         TTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGA                                                                        >  SRR19973073.2366303/1‑150 (MQ=60)
                                                                          TGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAA                                                                       <  SRR19973073.4196791/150‑1 (MQ=60)
                                                                               TGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGG                                                                  <  SRR19973073.793779/150‑1 (MQ=60)
                                                                               TGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGG                                                                  >  SRR19973073.802950/1‑150 (MQ=60)
                                                                                 CTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGAT                                                                <  SRR19973073.4304287/150‑1 (MQ=60)
                                                                                    ATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATC                                                             <  SRR19973073.407636/150‑1 (MQ=60)
                                                                                    ATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATC                                                             <  SRR19973073.407708/150‑1 (MQ=60)
                                                                                    ATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATC                                                             <  SRR19973073.407896/150‑1 (MQ=60)
                                                                                    ATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATC                                                             >  SRR19973073.943325/1‑150 (MQ=60)
                                                                                    ATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATC                                                             >  SRR19973073.331846/1‑150 (MQ=60)
                                                                                    ATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATC                                                             >  SRR19973073.2228111/1‑150 (MQ=60)
                                                                                      TAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGG                                                           >  SRR19973073.4024035/1‑150 (MQ=60)
                                                                                      TAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGG                                                           >  SRR19973073.1694602/1‑150 (MQ=60)
                                                                                      TAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGG                                                           >  SRR19973073.1694478/1‑150 (MQ=60)
                                                                                         CTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCT                                                        <  SRR19973073.4342557/150‑1 (MQ=60)
                                                                                          TCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTG                                                       <  SRR19973073.2172530/150‑1 (MQ=60)
                                                                                           CGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGC                                                      >  SRR19973073.3597712/1‑150 (MQ=60)
                                                                                           CGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGC                                                      >  SRR19973073.3597660/1‑150 (MQ=60)
                                                                                           CGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGC                                                      >  SRR19973073.3597584/1‑150 (MQ=60)
                                                                                            GGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCT                                                     <  SRR19973073.3722322/150‑1 (MQ=60)
                                                                                            GGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCT                                                     <  SRR19973073.1931824/150‑1 (MQ=60)
                                                                                            GGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCT                                                     >  SRR19973073.3918701/1‑150 (MQ=60)
                                                                                            GGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCT                                                     >  SRR19973073.3918675/1‑150 (MQ=60)
                                                                                            GGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCT                                                     >  SRR19973073.3918668/1‑150 (MQ=60)
                                                                                                   TTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATA                                              >  SRR19973073.3479767/1‑150 (MQ=60)
                                                                                                   TTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATA                                              <  SRR19973073.4282865/150‑1 (MQ=60)
                                                                                                   TTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGAGATTATGTCGGTGACCTTTGCAGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATA                                              >  SRR19973073.3479770/1‑150 (MQ=60)
                                                                                                     TTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATT                                            <  SRR19973073.3086429/150‑1 (MQ=60)
                                                                                                     TTGCTGCGGGATCGTCACATTATTGCAATGTATAGGTATCGGGAGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATT                                            <  SRR19973073.589646/150‑1 (MQ=60)
                                                                                                      TGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTT                                           <  SRR19973073.1670109/150‑1 (MQ=60)
                                                                                                         TGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGT                                        >  SRR19973073.1035654/1‑150 (MQ=60)
                                                                                                         TGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGT                                        <  SRR19973073.2079966/150‑1 (MQ=60)
                                                                                                         TGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGT                                        >  SRR19973073.3096731/1‑150 (MQ=60)
                                                                                                         TGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGT                                        >  SRR19973073.3096461/1‑150 (MQ=60)
                                                                                                            GGGATCGTCACATTCTTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCC                                     >  SRR19973073.1267468/1‑150 (MQ=60)
                                                                                                            GGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCC                                     <  SRR19973073.1097817/150‑1 (MQ=60)
                                                                                                            GGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCC                                     <  SRR19973073.1960268/150‑1 (MQ=60)
                                                                                                            GGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCC                                     >  SRR19973073.3029623/1‑150 (MQ=60)
                                                                                                            GGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCC                                     >  SRR19973073.3029671/1‑150 (MQ=60)
                                                                                                             GGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCA                                    <  SRR19973073.2340306/150‑1 (MQ=60)
                                                                                                             GGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCA                                    <  SRR19973073.1479357/150‑1 (MQ=60)
                                                                                                                TCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGACGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGAAATCGGCCTGCTGGGGATATTTGGTGCCACTA                                 >  SRR19973073.764453/1‑150 (MQ=60)
                                                                                                                 CGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTAT                                >  SRR19973073.4359961/1‑150 (MQ=60)
                                                                                                                  GTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATC                               <  SRR19973073.1108765/150‑1 (MQ=60)
                                                                                                                  GTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATC                               <  SRR19973073.1117161/150‑1 (MQ=60)
                                                                                                                  GTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATC                               <  SRR19973073.1108893/150‑1 (MQ=60)
                                                                                                                   TCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCG                              >  SRR19973073.167886/1‑150 (MQ=60)
                                                                                                                    CACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGC                             <  SRR19973073.2586767/150‑1 (MQ=60)
                                                                                                                     ACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCC                            >  SRR19973073.365398/1‑150 (MQ=60)
                                                                                                                     ACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCC                            >  SRR19973073.365618/1‑150 (MQ=60)
                                                                                                                          ATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGG                       >  SRR19973073.1400803/1‑150 (MQ=60)
                                                                                                                           TTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGT                      >  SRR19973073.4490138/1‑150 (MQ=60)
                                                                                                                             GCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTT                    <  SRR19973073.4133735/150‑1 (MQ=60)
                                                                                                                               AATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTA                  >  SRR19973073.4501334/1‑150 (MQ=60)
                                                                                                                                ATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTAT                 <  SRR19973073.2480719/150‑1 (MQ=60)
                                                                                                                                  GTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCA               <  SRR19973073.858832/150‑1 (MQ=60)
                                                                                                                                   TATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCAC              >  SRR19973073.4559613/1‑150 (MQ=60)
                                                                                                                                   TATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCAC              >  SRR19973073.3027815/1‑150 (MQ=60)
                                                                                                                                    ATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACC             <  SRR19973073.2758436/150‑1 (MQ=60)
                                                                                                                                     TCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCA            <  SRR19973073.4616220/150‑1 (MQ=60)
                                                                                                                                     TCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCA            <  SRR19973073.3224084/150‑1 (MQ=60)
                                                                                                                                     TCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCA            >  SRR19973073.3601489/1‑150 (MQ=60)
                                                                                                                                          ATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACATTA       <  SRR19973073.162524/150‑1 (MQ=60)
                                                                                                                                           TCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACATTAT      <  SRR19973073.3603853/150‑1 (MQ=60)
                                                                                                                                               GCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACATTATTAGC  <  SRR19973073.3084316/150‑1 (MQ=60)
                                                                                                                                               |                                                                                                                                                     
CTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACATTATTAGC  >  CP009273/3024840‑3025132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑