Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	46	290.7	5144628	99.3%	5108615	130.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AssemblyC74_contig_12	59,182	C→T	L52L (TTG→TTA)	rasttk_feature_annotation_tool=annotate_proteins_km er_v2 ←	Putative peptidoglycan bound protein (LPXTG motif) Lmo2178 homolog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AssemblyC74_contig_12	59,182	0	C	T	81.2%	2642.0 / inf	1216	L52L (TTG→TTA)	rasttk_feature_annotation_tool=annotate_proteins_km er_v2	Putative peptidoglycan bound protein (LPXTG motif) Lmo2178 homolog
Reads supporting (aligned to +/- strand): ref base C (109/117); new base T (497/481); total (617/599)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1217 total aligned reads displayed.

CTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGC  >  AssemblyC74_contig_12/59049‑59304

  tgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt                                                                                                                         >  2:2245960‑M1/9‑135 (MQ=255)

  tgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt                                                                                                                         >  1:663141‑M1/9‑135 (MQ=255)

  tgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt                                                                                                                         >  2:2496264‑M1/9‑135 (MQ=255)

  tgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt                                                                                                                         >  2:1979594‑M1/9‑135 (MQ=255)

  tgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGCCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt                                                                                                                         >  2:530732‑M1/9‑135 (MQ=255)

    caggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTc                                                                                                                       >  1:1780582‑M1/7‑135 (MQ=255)

    caggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTc                                                                                                                       >  2:1215507‑M1/7‑135 (MQ=255)

    CAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTc                                                                                                                       >  1:2283132‑M1/1‑135 (MQ=255)

    CAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTc                                                                                                                       >  1:596974‑M1/1‑135 (MQ=255)

    CAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTc                                                                                                                       >  1:1207800‑M1/1‑135 (MQ=255)

          ttCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATAccc                                                                                                                 >  2:63878/1‑135 (MQ=255)

          ttCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATAccc                                                                                                                 >  2:2543516/1‑135 (MQ=255)

          ttCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATAccc                                                                                                                 <  2:1257491/135‑1 (MQ=255)

          ttCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTAACACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATAccc                                                                                                                 >  1:2144117/1‑135 (MQ=255)

             tcCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCAAATTCATACCCCGt                                                                                                              >  2:1470384/1‑135 (MQ=255)

             cccGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATAccctat                                                                                                              >  2:804575/2‑132 (MQ=255)

             cccGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATAccctat                                                                                                              >  2:101199/2‑132 (MQ=255)

             cccGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATAccctat                                                                                                              >  1:1134306/2‑132 (MQ=255)

               cGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATAccctatt                                                                                                             >  2:1695612/1‑130 (MQ=255)

               cGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATAccctatt                                                                                                             >  2:1565839/1‑130 (MQ=255)

                 cTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGgc                                                                                                          <  1:409937/135‑1 (MQ=255)

                   ttGGTCATCGATCTTTGTCAACACGACACCGCCTGGTTTTAGTAGGTTTTCTTTCGTAAATTATACCACCGCTTTCTGAATTAATTCGATATCTAACTAGATTGGTGTCGCAACTAATTCATACCCTATTGgcg                                                                                                         <  2:92364/134‑1 (MQ=255)

                     ggTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCtt                                                                                                      <  1:522235/135‑1 (MQ=255)

                           cGATGTTTGTCAAATCGAAACAGCCTGGTGATAGTCGATTTTCTTTCGTCAATTATACCTCCGCTTCATGAATTCTTTCGATCTCTAAATCTATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGttt                                                                                                <  1:894937/135‑1 (MQ=21)

                           cGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGACAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGt                                                                                                  >  2:851268/1‑133 (MQ=255)

                           cGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTTTATTCTTTAGTTCTGAAAGGAGATTGGTTTCCCATCCAATTCATACCCCGTTGGCGCTTGGGttt                                                                                                <  1:1100848/135‑1 (MQ=255)

                            gATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTc                                                                                               <  1:2419251/135‑1 (MQ=255)

                            gATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTc                                                                                               <  2:141490/135‑1 (MQ=255)

                             aTCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCt                                                                                              <  2:229532/135‑1 (MQ=255)

                              tCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCt                                                                                              >  2:2092642/1‑134 (MQ=255)

                               cTTTGTCAACACGACACCGCCTGGTGTAAGTCGTTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTAc                                                                                            <  2:2195537/135‑1 (MQ=39)

                               cTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTAc                                                                                            <  2:1296168/135‑1 (MQ=39)

                                      accacGACACCGCCTGGTGTCCGTCGGTTTACCATCGTCACTTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCTCTTCTAATTCTGACCCTAATTGCGCTTGAGTTTCTACCAGCTg                                                                                      <  1:1267424/132‑1 (MQ=255)

                                      aaCACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTg                                                                                      <  2:484812/134‑1 (MQ=38)

                                      aaCACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTg                                                                                      <  2:754158/134‑1 (MQ=255)

                                      aaCACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTg                                                                                      <  1:798159/134‑1 (MQ=38)

                                      aaCACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTg                                                                                      <  1:557847/134‑1 (MQ=255)

                                       acacGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGAt                                                                                    <  1:1603627/135‑1 (MQ=255)

                                       acacGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGAt                                                                                    <  1:365003/135‑1 (MQ=38)

                                       acacGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGAt                                                                                    <  1:1368318/135‑1 (MQ=255)

                                        cacGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATa                                                                                   >  1:19315/1‑135 (MQ=38)

                                           gACACCTTCTGGTGTATGTCGATTTTCTTTCGTCAATTCTACCACCTCTGTCTGTATTCTTTCGCTCTCTAAATCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATacac                                                                                <  2:1601642/135‑1 (MQ=25)

                                           gACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATatgc                                                                                <  2:1365572/135‑4 (MQ=37)

                                                cGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTg                                                                           <  2:1779004/135‑1 (MQ=25)

                                                  ccTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGt                                                                         <  1:1162606/135‑1 (MQ=255)

                                                     ggTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGTTCCTGGTGCt                                                                      >  2:1916454/1‑135 (MQ=37)

                                                     ggTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTAc                                                                                            >  2:1818560/1‑113 (MQ=255)

                                                     ggTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCt                                                                      >  1:1055869/1‑135 (MQ=25)

                                                     ggTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTAc                                                                                            <  2:1114535/113‑1 (MQ=255)

                                                        gtAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTaa                                                                    >  1:853381/1‑134 (MQ=255)

                                                          aaGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCtt                                                                                                      <  2:1745625/98‑1 (MQ=255)

                                                          aaGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAAcc                                                                 >  2:388662/1‑135 (MQ=25)

                                                          aaGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAAcc                                                                 >  1:1819859/1‑135 (MQ=25)

                                                          aaGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAAcc                                                                 >  1:474354/1‑135 (MQ=25)

                                                            gTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt                                                                                                                         <  2:87893/77‑1 (MQ=255)

                                                              cggttttCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCa                                                             >  1:2082626/4‑135 (MQ=21)

                                                              cggttttCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTAc                                                                                            <  1:754865/101‑1 (MQ=37)

                                                               ggttttCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATatgc                                                                                >  1:2232160/3‑112 (MQ=25)

                                                               ggttttCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTc                                                              <  1:2035358/131‑1 (MQ=21)

                                                                   ttctttcGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattg                                                         >  2:57450‑M2/1‑130 (MQ=255)

                                                                   ttctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGTTCCTGGTGCTAAACCGTCAattgc                                                        <  2:2010854‑M2/135‑6 (MQ=255)

                                                                    tctttcGTCAATTCTACCACCGCTGTCTTACTTCTTTCGATCTCAAACTCGATTTGTGTCGCATATAATTAATACCCTATTTGCGCTTGGGTTTCTACAATCTGATATGTTCCTGGTGCTAAACCGTCAATTGcc                                                       >  1:2458507/1‑135 (MQ=255)

                                                                    tctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGTTCCTGGTGCTAAACCGTCAattgcc                                                       <  2:1857913‑M2/135‑7 (MQ=255)

                                                                    tctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgcc                                                       <  2:2132761‑M2/135‑7 (MQ=255)

                                                                    tctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAatcgcc                                                       <  2:1179886‑M2/135‑7 (MQ=255)

                                                                    tctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgct                                                       <  1:716521‑M2/135‑7 (MQ=255)

                                                                    tctttcGTCAATTCTACCACCGCTGTCTGACTTCTTACGATCTCAAACTCGATCGGTGTCGCATCTAATTCATAACCTATTGGCGCTTGAGTTTCTACAAGCTGATATGCTCCTGGTGCTAAACCGTCAattgct                                                       <  2:2049924‑M2/135‑7 (MQ=255)

                                                                             aaTTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGTTCCTGGTGCTAAACCGTCAattgccagttttcc                                               >  2:332272‑M2/1‑120 (MQ=255)

                                                                             aaTTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGTTCCTGGTGCTAAACCGTCAattgccagttttcc                                               >  1:1647876‑M2/1‑120 (MQ=255)

                                                                             aaTTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttcc                                               >  2:1514342‑M2/1‑120 (MQ=255)

                                                                              aTTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGTTCCTGGTGCTAAACCGTCAattgccagttttccat                                             >  2:684105‑M2/1‑119 (MQ=255)

                                                                              aTTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGTTCCTGGTGCTAAACCGTCAattgccagttttccat                                             >  1:2564474‑M2/1‑119 (MQ=255)

                                                                              aTTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccat                                             >  1:2328562‑M2/1‑119 (MQ=255)

                                                                              aTTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccat                                             >  1:1669554‑M2/1‑119 (MQ=255)

                                                                              aTTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccat                                             >  1:939448‑M2/1‑119 (MQ=255)

                                                                              aTTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAatcgccagttttccat                                             >  1:1450865‑M2/1‑119 (MQ=255)

                                                                                   accaccGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttct                                        >  2:420184‑M2/1‑114 (MQ=255)

                                                                                   accaccGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttct                                        >  1:1987874‑M2/1‑114 (MQ=255)

                                                                                       ccGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt                                                                                                                         >  2:1650818/1‑50 (MQ=255)

                                                                                       ccGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctg                                    <  1:2507292‑M2/135‑26 (MQ=255)

                                                                                            gTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt                                                                                                                         >  2:2156667/1‑45 (MQ=255)

                                                                                            gTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTc                                                                                                                       <  1:329947/47‑1 (MQ=255)

                                                                                            gTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGTTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctgttgtt                               <  1:2385682‑M2/135‑31 (MQ=255)

                                                                                            gTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctgtagtt                               >  1:100120‑M2/1‑105 (MQ=255)

                                                                                            gTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTAGCATCTAATTCATACCCTATTGTCGATTGAGTTTCTACCAGCTGATATGCTCCTGGTGATAAACCGTCAATTGCCAGTTTTCCTTCTTCTCCTGTAGtt                               <  1:1394745/135‑1 (MQ=17)

                                                                                            gTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAATTGCTAGTTTTCCATCTTCTCCTGTAGtt                               <  1:1742237/135‑1 (MQ=17)

                                                                                            gTCTGACTTCTTACGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAATTGCCAGTTTTCCATCTTCTCCTGTAGtt                               <  2:1049585/135‑1 (MQ=17)

                                                                                             tCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt                                                                                                                         <  1:1709661/44‑1 (MQ=255)

                                                                                             tCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATatgt                                                                                <  1:1555979/85‑4 (MQ=255)

                                                                                             tCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAATTGCTAGTTTTCCAtcttct                                        >  2:2349845/1‑125 (MQ=17)

                                                                                             tCTGACTTCTTTCGATCTCAAACTCGATAGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATatgc                                                                                <  1:1021719/85‑4 (MQ=18)

                                                                                               tGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAATTGCCAGTTTTCCATCTTCTCCTGTAGTTAa                             >  2:2424407‑M1/1‑133 (MQ=255)

                                                                                                     cttTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAAtt                                                          <  1:14809/99‑2 (MQ=17)

                                                                                                     cttTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAATTGCCAGTTTTCCATCTTCTCCTGTAGTTAGAccggtt                      <  2:717801/135‑5 (MQ=14)

                                                                                                     cttTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAATTGCTAGTTTTCCATCTTCTCCTGTAGTTAGAccggtt                      >  2:273183/1‑131 (MQ=17)

                                                                                                        tCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATatgc                                                                                <  1:2178377/74‑4 (MQ=17)

                                                                                                        tCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAATTGCCAGTTTTCCAt                                             >  2:2393120/1‑109 (MQ=14)

                                                                                                          gATCTCAAACTCGATTGGTGTCGCATCTAATTCAt                                                                                                                     >  2:2469783/1‑35 (MQ=255)

                                                                                                                         tGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAatcgccagttttccatctgctcctgtggttagacctgactgcagaacggttccattggc  <  1:1894689‑M2/135‑60 (MQ=255)

                                                                                                                                   tCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAatcgccagttttccatctgctcctgtggttagacctgactgcagaacg             >  1:1314754‑M2/1‑66 (MQ=255)

CTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGC  >  AssemblyC74_contig_12/59049‑59304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A