Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	47	403.1	7099244	99.3%	7049549	128.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AssemblyC74_contig_12	59,182	C→T	L52L (TTG→TTA)	rasttk_feature_annotation_tool=annotate_proteins_km er_v2 ←	Putative peptidoglycan bound protein (LPXTG motif) Lmo2178 homolog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AssemblyC74_contig_12	59,182	0	C	T	77.4%	3647.4 / inf	1915	L52L (TTG→TTA)	rasttk_feature_annotation_tool=annotate_proteins_km er_v2	Putative peptidoglycan bound protein (LPXTG motif) Lmo2178 homolog
Reads supporting (aligned to +/- strand): ref base C (216/216); new base T (759/718); total (978/937)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1916 total aligned reads displayed.

CTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCT  >  AssemblyC74_contig_12/59049‑59308

CTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCa                                                                                                                               <  1:3176908‑M1/135‑1 (MQ=255)

  tgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt                                                                                                                             >  2:3001679‑M1/9‑135 (MQ=255)

  tgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt                                                                                                                             >  2:2660191‑M1/9‑135 (MQ=255)

  cgCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACCGTACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCACTTCTCACTCAGAGGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAAt                                                                                                                             <  2:2082381/134‑1 (MQ=255)

  TGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAAt                                                                                                                             >  2:2787650‑M1/1‑135 (MQ=255)

  TGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAAt                                                                                                                             >  2:1121481‑M1/1‑135 (MQ=255)

    caggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTc                                                                                                                           >  2:1546544‑M1/7‑135 (MQ=255)

     aggatTTCCCCGCATTGGTCATCGATCTTTGTCAACACTACACCGCCTGGTGTAAGTCGGTTTTCTGTCGACAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCa                                                                                                                          <  1:2642046‑M1/130‑1 (MQ=255)

          ttCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATAccc                                                                                                                     <  2:839728/135‑1 (MQ=255)

             tcCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGTTGAACTTCGAGAATAATTACTCAAAACTCGAAACGAACACTGTCTACTTTCGTTATCAAACTCGATAGTTGTCGCATCCAATTCATACcgcgg                                                                                                                  >  2:696288/1‑134 (MQ=255)

               cGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATAGGTGTCGCATCTAATTCATAccctatt                                                                                                                 >  2:3325911/1‑130 (MQ=255)

               cGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGtt                                                                                                                 >  2:3465140/1‑134 (MQ=255)

               cGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGtt                                                                                                                 >  2:3255176/1‑134 (MQ=255)

               cGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATAGGTGTCGCATCTAATTCATAccctatt                                                                                                                 >  1:922498/1‑130 (MQ=255)

                 cTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGgc                                                                                                              <  1:2358712/135‑1 (MQ=255)

                 cTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGgc                                                                                                              <  2:567788/135‑1 (MQ=255)

                   ttGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGgcg                                                                                                             <  1:3528136/134‑1 (MQ=255)

                    tGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCt                                                                                                           >  2:1974184/1‑135 (MQ=255)

                         aTCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAAt                                                                                                                             >  2:2528356/1‑112 (MQ=255)

                         aTCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGgc                                                                                                              >  2:1918111/1‑127 (MQ=255)

                          tCGATCTTTCTCAACACGACACCGCCTCGTGTAAGTCTTTTTTCTTTCGTTCATTCTACCACCTCTGTCTGACTAATTCCTATCTATATATCGATTTGTCTATCAGATAATTCATACCCTATTGGCGCTTGAGtt                                                                                                     <  2:449113/135‑1 (MQ=255)

                             aTCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCt                                                                                                  <  1:1294506/135‑1 (MQ=39)

                             aTCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCt                                                                                                  <  2:624135/135‑1 (MQ=39)

                             aTCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCt                                                                                                  <  2:756985/135‑1 (MQ=255)

                              tCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCt                                                                                                  >  2:272634/1‑134 (MQ=39)

                               cTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTAc                                                                                                <  1:1061094/135‑1 (MQ=255)

                               cTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTAc                                                                                                <  2:1392399/135‑1 (MQ=39)

                               cTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTAc                                                                                                <  1:683865/135‑1 (MQ=39)

                               cTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTAc                                                                                                <  1:2241620/135‑1 (MQ=39)

                               cTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTAc                                                                                                <  2:3191466/135‑1 (MQ=39)

                               cTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTAc                                                                                                <  1:3009424/135‑1 (MQ=255)

                                     cAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTg                                                                                          >  1:489189/1‑135 (MQ=38)

                                      accacGACACCGCCAGGTGTAAGTCTGTATTCGTTCGACAACTCGACCACCTCTGTCTGAATTCGTTCTATCTCAATCTCGATTGGTGTAGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTg                                                                                          <  1:2943669/132‑1 (MQ=255)

                                      aaCACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTg                                                                                          <  1:3285775/134‑1 (MQ=38)

                                      aaCACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTg                                                                                          <  1:1524005/134‑1 (MQ=38)

                                      aaCACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTg                                                                                          <  1:3230812/134‑1 (MQ=255)

                                       acacGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGAt                                                                                        <  1:990680/135‑1 (MQ=255)

                                           gACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCGTCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATatgc                                                                                    <  1:539371/135‑4 (MQ=37)

                                           gACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATatgt                                                                                    <  2:203693/135‑4 (MQ=38)

                                           gACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATatgt                                                                                    <  1:2061743/135‑4 (MQ=38)

                                           gACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATatgt                                                                                    <  1:2489407/135‑4 (MQ=38)

                                           gACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATatg                                                                                     >  2:2442238/1‑132 (MQ=37)

                                           gACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATGGGTGTCGCATCTAATTCATACCCTTTTGGCGCTTGTGTTTCTACCAGCTGATatgc                                                                                    <  1:1808538/135‑4 (MQ=37)

                                           gACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATatg                                                                                     >  1:340909/1‑132 (MQ=37)

                                           gACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCGACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATatgt                                                                                    <  1:3353262/135‑4 (MQ=38)

                                           gACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATacac                                                                                    <  1:2310783/135‑1 (MQ=255)

                                                     ggTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCt                                                                          >  1:2424912/1‑135 (MQ=25)

                                                     ggTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCt                                                                          >  1:2174870/1‑135 (MQ=25)

                                                      gtgtAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGTTCCTGGTGCTa                                                                         <  1:34729/135‑1 (MQ=25)

                                                        gtAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATatgc                                                                                    <  1:1592213/122‑4 (MQ=25)

                                                        gtAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTaa                                                                        >  2:3127370/1‑134 (MQ=25)

                                                          aaGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGgcg                                                                                                             >  2:3399843/1‑95 (MQ=255)

                                                              cGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCa                                                                 >  1:1660334/1‑135 (MQ=255)

                                                               ggttttctTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAa                                                                <  2:2136651‑M2/133‑2 (MQ=255)

                                                               ggttttCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGa                                                                                         <  1:1130651/108‑1 (MQ=255)

                                                               ggttttCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCt                                                                                                  <  2:1058252/99‑1 (MQ=38)

                                                               gATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCt                                                                                                  >  2:2846980/1‑101 (MQ=255)

                                                               gATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCt                                                                                                  <  2:2295269/101‑1 (MQ=255)

                                                                   ttctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgc                                                            <  1:311839‑M2/135‑6 (MQ=255)

                                                                   ttctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAatcgc                                                            <  2:1321980‑M2/135‑6 (MQ=255)

                                                                   ttctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgc                                                            <  1:444410‑M2/135‑6 (MQ=255)

                                                                    tctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGTTCCTGGTGCTAAACCGTCAattgcc                                                           <  2:1203321‑M2/135‑7 (MQ=255)

                                                                    tctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTAc                                                                                                <  1:1001082/98‑1 (MQ=37)

                                                                    tctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgcc                                                           <  1:2547385‑M2/135‑7 (MQ=255)

                                                                    tctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgcc                                                           <  1:2751733‑M2/135‑7 (MQ=255)

                                                                    tctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAatcgcc                                                           <  2:3537090‑M2/135‑7 (MQ=255)

                                                                    tctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATAGGTGTCGCATCCAAATCATACCCAGTTGGCGCTTGGGTTTCTACCAGCTGATAATCTCCTGGTGCTAAACCGTCAatagca                                                           <  2:1690914‑M2/135‑7 (MQ=255)

                                                                       ttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagt                                                        >  2:1750919‑M2/1‑126 (MQ=255)

                                                                       ttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagt                                                        >  1:804307‑M2/1‑126 (MQ=255)

                                                                       ttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAatcgccagt                                                        >  1:397457‑M2/1‑126 (MQ=255)

                                                                             aaTTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgctagttttcc                                                   >  1:60674‑M2/1‑120 (MQ=255)

                                                                              aTTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGTTCCTGGTGCTAAACCGTCAattgccagttttccat                                                 >  2:2592499‑M2/1‑119 (MQ=255)

                                                                              aTTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccat                                                 >  2:236069‑M2/1‑119 (MQ=255)

                                                                              aTTCTACCACCGCGGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAATTGCTAGTTTTCCAt                                                 <  2:2921259/135‑1 (MQ=18)

                                                                                  tACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGTTCCTGGTGCTAAACCGTCAattgccagttttccatcttc                                             >  1:848361‑M2/1‑115 (MQ=255)

                                                                                  tACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGTTCCTGGTGCTAAACCGTCAattgccagttttccatcttc                                             >  1:2864236‑M2/1‑115 (MQ=255)

                                                                                  tACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttc                                             >  2:509059‑M2/1‑115 (MQ=255)

                                                                                   accaccGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttct                                            >  1:1439397‑M2/1‑114 (MQ=255)

                                                                                   accaccGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAatcgccagttttccatctgct                                            >  1:136672‑M2/1‑114 (MQ=255)

                                                                                   aacaccgcTGTCTGAATTCTGTCTCAGTCACACTCGATTTGTGTCGCCTCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAatcgccagttttccatctgct                                            >  2:1464094‑M2/3‑114 (MQ=255)

                                                                                       ccGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATatgc                                                                                    <  1:2598436/91‑4 (MQ=21)

                                                                                          cTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttct                                            >  2:1613518‑M2/1‑107 (MQ=255)

                                                                                            gTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGTTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctgtagtt                                   >  2:1260629‑M2/1‑105 (MQ=255)

                                                                                            gTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctgtagtt                                   <  1:754040‑M2/135‑31 (MQ=255)

                                                                                            gTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctgtagtt                                   >  1:307071‑M2/1‑105 (MQ=255)

                                                                                            gTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctgtagtt                                   >  1:2794866‑M2/1‑105 (MQ=255)

                                                                                            gTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAATTGCTAGTTTTCCATCTTCTCCTGTAGtt                                   <  2:934925/135‑1 (MQ=17)

                                                                                             tCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGTTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctgtagtta                                  <  1:1722297‑M2/135‑32 (MQ=255)

                                                                                             tCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctgtagtta                                  <  1:1997232‑M2/135‑32 (MQ=255)

                                                                                             tCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctgtagtta                                  <  2:1836778‑M2/135‑32 (MQ=255)

                                                                                             tCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctgtagtt                                   >  1:1933958‑M2/1‑104 (MQ=255)

                                                                                             tCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAATTGCTAGTTTTCCATCTTCTCCTGTAGTTa                                  >  1:1185904/1‑135 (MQ=17)

                                                                                                 aCTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctgtagttagacc                              <  1:1857721‑M2/135‑36 (MQ=255)

                                                                                                 aCTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCa                                                                                                                          >  2:1797627/1‑43 (MQ=255)

                                                                                                       ttCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAatcgccagttttccatctgctcctgtggttagacctgactg                        >  2:2227470‑M2/1‑94 (MQ=255)

                                                                                                          gATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATatgt                                                                                    >  1:2713114/1‑69 (MQ=18)

                                                                                                          gATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGTTCCTGGTGCTAAACCGTCAATTGCCAGTTTTCCATCTTCTCCTGTAGtt                                   <  2:3060157/121‑1 (MQ=14)

                                                                                                          gATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAatcgccagttttccatctgctcctgtggttagacctgactgcag                     <  1:2100416‑M2/135‑45 (MQ=255)

                                                                                                                           gtgtCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATacac                                                                                    >  1:1772102/1‑55 (MQ=255)

                                                                                                                              tCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAatcgccagttttccatctgctcctgtggttagacctgactgcagaacggttccattggcatct  >  2:40325‑M2/1‑71 (MQ=255)

CTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCT  >  AssemblyC74_contig_12/59049‑59308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A