Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R2 44 314.5 5848508 99.3% 5807568 128.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA AssemblyC74_contig_12 59,182 C→T L52L (TTG→TTA)  rasttk_feature_annotation_tool=annotate_proteins_km er_v2 ← Putative peptidoglycan bound protein (LPXTG motif) Lmo2178 homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AssemblyC74_contig_1259,1820CT80.3% 3158.6 / inf 1504L52L (TTG→TTA) rasttk_feature_annotation_tool=annotate_proteins_km er_v2Putative peptidoglycan bound protein (LPXTG motif) Lmo2178 homolog
Reads supporting (aligned to +/- strand):  ref base C (157/138);  new base T (620/585);  total (780/724)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01

Only 100 of 1505 total aligned reads displayed.

CTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTG  >  AssemblyC74_contig_12/59049‑59302
                                                                                                                                     |                                                                                                                        
  tgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt                                                                                                                       >  1:1802635‑M1/9‑135 (MQ=255)
  tgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt                                                                                                                       >  2:1915352‑M1/9‑135 (MQ=255)
  tgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt                                                                                                                       >  1:1993455‑M1/9‑135 (MQ=255)
  tgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt                                                                                                                       >  2:2365289‑M1/9‑135 (MQ=255)
  tgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt                                                                                                                       >  2:716497‑M1/9‑135 (MQ=255)
  TGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAAt                                                                                                                       >  1:2214676‑M1/1‑135 (MQ=255)
   GCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAAtt                                                                                                                      >  1:1840481‑M1/1‑135 (MQ=255)
    caggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTc                                                                                                                     >  1:113656‑M1/7‑135 (MQ=255)
     aggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCa                                                                                                                    <  1:2322701‑M1/130‑1 (MQ=255)
          ttCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATAccc                                                                                                               <  2:313984/135‑1 (MQ=255)
          ttCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATAccc                                                                                                               <  2:1540918/135‑1 (MQ=255)
             cccGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATAccctat                                                                                                            >  2:433188/2‑132 (MQ=255)
               cGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGtt                                                                                                           >  1:1086573/1‑134 (MQ=255)
               cGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGtt                                                                                                           >  2:800703/1‑134 (MQ=255)
                 gtttGCTCAGCGATCTGTGTCTACTCGACACCGCGTGGTGTATCACGCTTTTCTTTCGTCAATTATACCTCCGCTGCCTGTCTTCTTTCGATCTCCAAAGCGACTGGTGTCGCATCCAATTCATACCCCGTTGgc                                                                                                        <  2:1579722/134‑1 (MQ=19)
                 cTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGgc                                                                                                        <  2:2431715/135‑1 (MQ=255)
                 cTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGgc                                                                                                        <  1:2526840/135‑1 (MQ=255)
                 cTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGgc                                                                                                        <  1:188668/135‑1 (MQ=255)
                    tGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGgcg                                                                                                       >  1:451319/1‑133 (MQ=255)
                         aTCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGt                                                                                                >  1:486030/1‑135 (MQ=255)
                         aTCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGt                                                                                                >  1:207947/1‑135 (MQ=255)
                             aTCTATGTCAACACGACACCGACTGGTGTAAGTCGATTTTCTTTAGTCAATTCTACCACCTCTGTCTGAATTCTTTCGATCACAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCt                                                                                            <  2:2690874/135‑1 (MQ=38)
                               cTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTAc                                                                                          <  2:119408/135‑1 (MQ=255)
                               cTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTAc                                                                                          <  2:2055315/135‑1 (MQ=39)
                                tttGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTAcc                                                                                         <  2:1027485/135‑1 (MQ=39)
                                  tGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAg                                                                                       <  2:2833379/135‑1 (MQ=38)
                                      aaCACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGa                                                                                   <  1:403229/135‑1 (MQ=255)
                                       acacGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGAt                                                                                  <  1:1410797/135‑1 (MQ=38)
                                           gACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATatgt                                                                              <  2:113583/135‑4 (MQ=38)
                                           gACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGTTatg                                                                               >  2:2134437/1‑132 (MQ=37)
                                           gACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATatgc                                                                              <  2:2188594/135‑4 (MQ=37)
                                           gACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATatgc                                                                              <  2:1512846/135‑4 (MQ=37)
                                           gACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt                                                                                                                       >  1:2919098/1‑94 (MQ=255)
                                           gACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTc                                                                                             >  1:1264531/1‑120 (MQ=255)
                                           gACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATatgc                                                                              <  1:1008366/135‑4 (MQ=37)
                                           gACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATatgc                                                                              <  2:1811709/135‑4 (MQ=37)
                                           gACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATatgc                                                                              <  2:2888993/135‑4 (MQ=37)
                                           gACACCGCCTGGTGTAAGTCGGTTTTAATTCGTCAATTCTACCACCGCTGTCTGAATACTTTCGATCTCCATCTAGATTGGTGTCGCATCGAATTGATAGCCTATTGGCGCGTGTGTTTCTACctgctggtgtg                                                                               >  2:2759716/1‑129 (MQ=255)
                                           gACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGTTCTCAAAATCGTTTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATacac                                                                              <  1:2028358/135‑1 (MQ=255)
                                           gACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATacac                                                                              <  2:764074/135‑1 (MQ=255)
                                           gACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATacac                                                                              <  1:920493/135‑1 (MQ=255)
                                                  ccTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGt                                                                       <  1:2677852/135‑1 (MQ=255)
                                                   cTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATatgc                                                                              >  2:2010828/1‑124 (MQ=37)
                                                     ggTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCt                                                                          >  1:972232/1‑129 (MQ=25)
                                                     ggTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCt                                                                    >  2:124482/1‑135 (MQ=25)
                                                        gtAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTc                                                                                                                     <  2:938588/83‑1 (MQ=255)
                                                        gtAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATatgc                                                                              >  1:851162/1‑119 (MQ=37)
                                                          aaGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGcc                                                               >  1:802537/1‑135 (MQ=255)
                                                           aGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCg                                                              <  2:1309832/135‑1 (MQ=255)
                                                               ggttttctTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGTGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAa                                                          <  1:1517653‑M2/133‑2 (MQ=255)
                                                               ggttttctTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAa                                                          <  1:1560128‑M2/133‑2 (MQ=255)
                                                               ggttttCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATatgt                                                                              >  2:2102627/3‑112 (MQ=37)
                                                               gATTGTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTc                                                            <  1:1044427/133‑1 (MQ=255)
                                                                   ttctttcGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgc                                                      <  1:1194708‑M2/135‑6 (MQ=255)
                                                                   ttctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGTTCCTGGTGCTAAACCGTCAattgc                                                      <  2:851091‑M2/135‑6 (MQ=255)
                                                                   ttctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGATAAACCGTCAattgc                                                      <  1:1667821‑M2/135‑6 (MQ=255)
                                                                   ttctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAatcgc                                                      <  2:567842‑M2/135‑6 (MQ=255)
                                                                    tctttcGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgcc                                                     <  2:16409‑M2/135‑7 (MQ=255)
                                                                    tctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt                                                                                                                       <  1:78228/69‑1 (MQ=255)
                                                                    tctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt                                                                                                                       >  1:2114185/1‑69 (MQ=255)
                                                                    tctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGgcg                                                                                                       >  2:1952709/1‑85 (MQ=255)
                                                                    tctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgcc                                                     <  1:2783900‑M2/135‑7 (MQ=255)
                                                                    tctttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgcc                                                     <  1:2171238‑M2/135‑7 (MQ=255)
                                                                       ttcGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGTTCCTGGTGCTAAACCGTCAattgccagt                                                  >  2:1743303‑M2/1‑126 (MQ=255)
                                                                         cGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGTTCCTGGTGCTAAACCGTCAattgccagttt                                                >  1:2848644‑M2/1‑124 (MQ=255)
                                                                             aaTTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttcc                                             >  1:1327186‑M2/1‑120 (MQ=255)
                                                                             aaTCCTACCACCGCTGTCTGACTACTTTCGATCACAAACTCGATTGGTTTCGCATCTATTTCCTTCCCTATTTGAGCTGGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAATTGCCAGTTTTcc                                             <  1:1460849/134‑1 (MQ=16)
                                                                              aTTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGTTCCTGGTGCTAAACCGTCAattgccagttttcc                                             <  1:750069‑M2/133‑15 (MQ=255)
                                                                              aTTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCt                                                                                            <  1:886523/86‑1 (MQ=37)
                                                                              aTTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccat                                           >  2:2592693‑M2/1‑119 (MQ=255)
                                                                                 cTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAatcgccagttttccatc                                          <  1:578897‑M2/133‑18 (MQ=255)
                                                                                  tACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTc                                                                                                                     >  1:2467428/1‑57 (MQ=255)
                                                                                   accaccGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttct                                      >  2:522625‑M2/1‑114 (MQ=255)
                                                                                   accaccGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttct                                      >  2:1081844‑M2/1‑114 (MQ=255)
                                                                                   accaccGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAatcgccagttttccatctgct                                      >  1:1942666‑M2/1‑114 (MQ=255)
                                                                                   accaccGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAatcgccagttttccatctgct                                      >  1:2602864‑M2/1‑114 (MQ=255)
                                                                                   accaccGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAatcgccagttttccatctgct                                      >  1:2717005‑M2/1‑114 (MQ=255)
                                                                                       ccGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATatgt                                                                              >  2:1466662/1‑88 (MQ=25)
                                                                                       ccGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTc                                                                                                                     >  2:1708142/1‑52 (MQ=255)
                                                                                       ccGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATAccc                                                                                                               <  2:893332/58‑1 (MQ=255)
                                                                                        cGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAatcgccagttttccatctgctcctgt                                 >  1:704652‑M2/1‑109 (MQ=255)
                                                                                          cTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATATGTTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctgtag                               <  2:2510729‑M2/135‑29 (MQ=255)
                                                                                          cTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctgtag                               <  1:1613617‑M2/135‑29 (MQ=255)
                                                                                            gTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctgtagtt                             >  2:2274819‑M2/1‑105 (MQ=255)
                                                                                            gTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctgtagtt                             >  1:345665‑M2/1‑105 (MQ=255)
                                                                                            gTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctgtagtt                             >  1:1694585‑M2/1‑105 (MQ=255)
                                                                                            gTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctgtagtt                             >  1:536953‑M2/1‑105 (MQ=255)
                                                                                            gTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAATTGCTAGTTTTCCATCTTCTCCTGTAGtt                             <  2:2641413/135‑1 (MQ=17)
                                                                                             tCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGTTCCTGGTGCTAAACCGTCAattgccagttttccat                                           <  1:2281263‑M2/120‑17 (MQ=255)
                                                                                             tCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctgtagtta                            <  1:1364515‑M2/135‑32 (MQ=255)
                                                                                             tCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATacac                                                                              <  2:231407/85‑1 (MQ=255)
                                                                                               tGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGGGTTTCTACCAGCTGATatgt                                                                              >  2:1845721/1‑80 (MQ=21)
                                                                                               tGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctgtagttag                           <  2:638145‑M2/134‑33 (MQ=255)
                                                                                                 aCTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAattgccagttttccatcttctcctgtagtt                             <  1:635306‑M2/130‑31 (MQ=255)
                                                                                                    tcttTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAATTGCCAGTTTTCCATCTTCTCCTGTAGTTAGAccggt                     <  2:392639/135‑4 (MQ=14)
                                                                                                     cttTCGATCTCAAACTCGATTGGTTACGCATCTAATTCATACCCTAATGGCGCTTGGGTTTCTACCAGCTGATATGATCCTGGTGATAAACCGTCAATTGCCAGTTTTCCATCTTATACTGTAGTTAAAccggtt                    >  2:141596/1‑131 (MQ=12)
                                                                                                     cttTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTc                                                                                             >  2:488170/1‑62 (MQ=255)
                                                                                                          gATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAatcgccagttttccatctgctcctgtggttagacctgactgcag               <  1:2414007‑M2/135‑45 (MQ=255)
                                                                                                              tcAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAACCGTCAATTGCCAGTTTTCCAt                                           >  2:1360921/1‑103 (MQ=14)
                                                                                                                       aTTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAatcgccagttttccatctgctcctgtggttagacctgactgcagaacggttccattg  >  1:1740076‑M2/1‑78 (MQ=255)
                                                                                                                                     |                                                                                                                        
CTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTTCTACCAGCTGATACACTCCTGGTGCTAAGCCGTCAATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTG  >  AssemblyC74_contig_12/59049‑59302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A