Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F9 I0 R1 113 83.2 1840812 91.6% 1686183 224.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 647,210 A→C 26.7% G100G (GGT→GGG)  citG ← 2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913647,2100AC26.7% 72.1 / 2.1 49G100G (GGT→GGG) citG2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase
Reads supporting (aligned to +/- strand):  ref base A (25/8);  new base C (4/11);  total (30/19)
Fisher's exact test for biased strand distribution p-value = 3.24e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTAAGGCCAAGCTGTTGGTACAACCGTTGACCTGC‑CGTCAGTTGTGAA‑TTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGCGCCAGCCCAGCAGGGCG  >  NC_000913/646979‑647447
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             
gggaaGGGCAAGTTGTTGGTACAAACGTTGGCCCGCGCGTAAGGT‑TGAATTTGTTGGAACGCGGTCGGGGGCGGGGCAGGCCCCGGCA‑AAACTT‑GCCCGCGGTAGAACAACCGTTTGTGGGCGTTCCCGTTTGGTTGAGTTGAGGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCg                                                                                                                                                                                                                                         <  2:918529/236‑1 (MQ=255)
         aaGCTGTTGGTACAACCGTTGACCTGC‑CGTCAGTTGTGAA‑TTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCCCGGAACCTATCACCTTCGCAAGCCATa                                                                                                                                                                                                                                >  1:526483/1‑238 (MQ=255)
           gCTGTTGGTACAACCGTTGACCTGC‑CGTCAGTTGTGAA‑TTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGACGCAAACGGCCAATTGCCGCAC‑ATATCAGCCCTAACGCAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAAAATATCACCTTTGCAAGCCATccc                                                                                                                                                                                                                              >  1:533492/1‑238 (MQ=255)
           gCTGTTGGTACAACCGTTGACCTGC‑CGTCAGTTGTGAA‑TTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTAGGCGTGTTTACGCCCGCAGTGGCGCGGCACATATCCCCTTCGCAAGCCTAACCa                                                                                                                                                                                                                             >  1:133614/1‑239 (MQ=255)
                tGGTACAACCGTTGACCTGC‑CGTCAGTTGTGAA‑TTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGGGTTTACGCCCGCAGAGGCGCGGAACACATAAcccagtcccttataaccatctcc                                                                                                                                                                                                                        >  1:324221/1‑218 (MQ=255)
                         cGTTGACCTGC‑CGTCAGTTGTGAA‑TTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACAATACCCCTTCCCAAGCCCTACCAATTGGGCGAAAAcc                                                                                                                                                                                                               >  2:546772/1‑239 (MQ=255)
                            tGACCTGC‑CTCGAGTTGTGAA‑TTATGGCTACGCATTTCGGGACCGGACAGGCCAGGGCAGAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTg                                                                                                                                                                                                            <  1:546772/239‑1 (MQ=255)
                                  gC‑CGTCAGTTGTGAA‑TTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTTGGCGTAATCCGTGGCGtc                                                                                                                                                                                                       >  1:892966/1‑237 (MQ=255)
                                  gC‑CGTCAGTTGTGAA‑TTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGGGGATTa                                                                                                                                                                                                       >  1:474356/1‑238 (MQ=255)
                                              tgAA‑TTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑G‑CCGCCGTAAAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAA‑CTACCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACCCCCCCCAGGACTGGGAACATATCTCATTCGAAAGCCATACCCATTGGCCAGAAGCCGGGGGGTACCGCCTCCgga                                                                                                                                                                                            >  2:27148/1‑237 (MQ=255)
                                                     tttgggccggaggtgggaatggttCAGGCAAGGGCA‑AAACTTGG‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCAttt                                                                                                                                                                                     <  1:649173/216‑1 (MQ=255)
                                                      ttGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACGCCTTCAGGGTCCAttt                                                                                                                                                                                     >  1:734125/1‑238 (MQ=255)
                                                      ttGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGGGGCACGGAACATAACACCTTCGCAAGCCATACCAATTGGGCGGAATCCGGGGAGAACGGCTTCTGGGGCCAttt                                                                                                                                                                                     >  2:462258/1‑238 (MQ=255)
                                                         gTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACCTACTCCCTTCGCAAGCCATTCCAATTGGCGGGAATCCGTGGGGAACCGCTCCTGGGGCCATTTCCGc                                                                                                                                                                                 >  2:73918/1‑239 (MQ=255)
                                                              cAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGGGCCATTTCCGAACTAc                                                                                                                                                                            >  1:760077/1‑239 (MQ=255)
                                                                 ttCGCGATCGGTCAGGCCACGGCAGAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAg                                                                                                                                                                          >  1:889146/1‑238 (MQ=255)
                                                                  tcggctggtgggcgtccccGGCAGAAAATT‑GCCCTCCGTAGACAAAACGGTTGTTGGCGTTACCGGTTGGTTGATGTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCGAAAGAAAAAATGCTGCCTTTATGCGTGTTTCCGCCCGCAGGGGCGCGGACCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAg                                                                                                                                                                          <  2:861922/220‑1 (MQ=255)
                                                                   cgcgTTCGGTCGGGCCCCGGCAAAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTCCCGGTGGGGTGATGTGAACCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTAGGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCa                                                                                                                                                                       <  1:462258/239‑1 (MQ=255)
                                                                   cgcgATCGGTCAGGCCACGGCAGAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCCCGGAACATATCACCTTCGCAAGCCATACCAATTGCGCGTAATCCTGGGGGACCCCCTTCTGGTGCCATTTCCGCCCTACAGGc                                                                                                                                                                        >  2:898482/1‑238 (MQ=255)
                                                                         cGGTCGGGCCACGGAAAAACCTT‑G‑CCGCGGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTCCGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCaaa                                                                                                                                                                  <  1:77716/238‑1 (MQ=255)
                                                                          ggccgagcccccggaaaaatgtcg‑ccgccTAAAAACAACGGGTTGTTGGTGTTACGGGTTGATTGAGTTGAAGCAAACGGCCAAGTGGCGCCA‑AGAGCAGATCTAAAGAAAAAATGCTGCTTTTACGCGTTTTTACGCCAGCAGGGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCaaa                                                                                                                                                                  <  2:133614/215‑1 (MQ=255)
                                                                           gTCAGGCCACGGCAGAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTCCAGGCACCAAATTc                                                                                                                                                               >  1:546796/1‑239 (MQ=255)
                                                                           gTCAGGCCACGGCAGAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATCCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGGAATCCGGGGAGTACCGCTTCTGGGGCCCTTTCCGCACCCAAGGCCCCAAAtga                                                                                                                                                               >  2:561433/1‑237 (MQ=255)
                                                                           gTCAGGCCACGGCAGAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAACAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGGTTACGCCCGCAGTGGCGCGGCACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGGGGGGAACCGCTTCCGGGGCCATTTCCGCACTACAGGCACCAAAATc                                                                                                                                                               >  2:280322/1‑239 (MQ=255)
                                                                           gTCAGGCCACGGCAGAAACTT‑G‑ACGCCGCAGAACAACCGGTTGTTGGCGCTACCGGTTGGTTGAGTTGAAGCAAACGGCCGATTGCCGCAC‑CTAGCAGCCCTCAAGAAAACATGCTGCTTTTAGTCGAGTGTACGCCCGCAGCGTCCGGGCACATTTTACCTCCGCCAGCCATACCACTTGTGCGTCATTCGCGGGGGACCGCTCCTGGTGCCATTTCCCCCCCCCCGGAACCAAATTc                                                                                                                                                               >  2:74692/1‑239 (MQ=255)
                                                                                     ggCAGAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCCCAGTGGCCCGGAACATATCACCTTCGCCAGCCATACCAATTGGGCGTAATCCGTGGAGAACCCCTTCTGGGGCCATTTCCCACCTACAGGCCCCCAATTCAATGAAAAgg                                                                                                                                                     >  2:735430/1‑239 (MQ=255)
                                                                                        gacatacttcgcctgtgTAGAAAAAGCGGTTTGTGGTGTTGCCGGGTGTGTGAGTAAAAGAAACCGGCTATTTGCC‑CACAATAGCAGCCTAAAAGAAAAAATGCTGCCTTTAGGCGTGTTTACGCCGGCGTGGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACggg                                                                                                                                                    <  2:776707/223‑1 (MQ=255)
                                                                                          aaaCTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGGGTACCGCTTCTGGGGCCATTTCCCCACTACAGGCACAAAATTCAATGAAAAGGGGtg                                                                                                                                                 >  2:474404/1‑237 (MQ=255)
                                                                                                             aCAAACGGTTGTTGGCGTTACGGGTTGGTTGAGTTGAAGAAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGc                                                                                                                                 <  1:474404/237‑1 (MQ=255)
                                                                                                                 aCGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGAAGCCAGCCCTGAATCGCCAgcc                                                                                                                            >  1:187677/1‑237 (MQ=255)
                                                                                                                     tttttggctgtcccGTTTGGTTGAGGTAAAGAAAACGGCAATTTCGCACAG‑ATGGACGCTCAAAAAAAAAAATGCTGCCTTTATGCGTGTTGACGCCCGCGGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTg                                                                                                                       <  2:804755/227‑1 (MQ=255)
                                                                                                                     tttgtgGCGTTACCGTTTTTTTGAGTAGAAGCAAACGGCCAATGCCCCCAA‑ATAGAGGCCAAAAAAAAAAAATGCTGCCTTTAGGCGTGTTTACGCCCGCAGGGGGCGGGAACATTCCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTg                                                                                                                       <  2:187677/235‑1 (MQ=255)
                                                                                                                       gttgGCGTTACCGGTTGGTTGAGTTGAAGCAAACGACCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGGGTACCGCTTCTGGTGCCATTTCCGCACTACCGGCACCAAATTCAATGAAACGGAGTGACCCGCCCCGAATCGCCacggccccgcg                                                                                                                     >  2:247082/1‑229 (MQ=255)
                                                                                                                         tgGGGTTACCGGTTGGTTGAGTTGAACCAAACGGCCAATTGCCGCAA‑ATAGCACCCCTAAAGAAAAAATGCTGCCTTTTGGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGt                                                                                                                   <  1:73918/239‑1 (MQ=255)
                                                                                                                               tACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCCCGCTGCGGTGGaaa                                                                                                              >  2:268495/1‑238 (MQ=255)
                                                                                                                                         tttgatgtgaacAAACGGGCAATTGGCACACAATAGCAGCCCTAAAAAAAAAATGGTGCTTTTAGGCGTGGTTACCCCCGAGGTGGCGCGGACCATATCCCTTTCCAAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAgcg                                                                                                    <  2:526483/228‑1 (MQ=255)
                                                                                                                                            agttgaAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTCCGGGGGAAATCTTCCAGCGCCat                                                                                                >  1:850604/1‑239 (MQ=255)
                                                                                                                                                           caaaTGGCCGCAC‑AAGGCAGCCCAAAAGAAAAAAGGCGGCCTTTAGGGGTGTTTACGCCCGAAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCAc                                                                                  <  1:735430/236‑1 (MQ=255)
                                                                                                                                                             aaTTGCCGCAC‑ATAGCCCCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGc                                                                               <  1:334644/239‑1 (MQ=255)
                                                                                                                                                                     cac‑aTAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTCCAGGCACCAAATTCCATGAAACGGGGAAGCCGGCCCTGAATCCCCAGCCCGCTCCGGTGGAAATCTTCCAGCGCCCAATCTTTGTTCGCCCCGCAGTTaaat                                                                       >  2:10241/1‑237 (MQ=255)
                                                                                                                                                                               cctcTAAAGAAAAATTTCTCCTTTTGGTGGTTTTTCCGCCCGTGGGGGCGCGAAACTTACCCCCCTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACg                                                              <  2:336413/236‑1 (MQ=255)
                                                                                                                                                                                cccTAAAAAAAAAAGTCTGCCTTTGTCGGGGTTTACGCCCGCAGTGGCGCGGAACTTTTCCCTTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACg                                                              <  1:10241/238‑1 (MQ=255)
                                                                                                                                                                                     aaGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATAGCTTTGTGCGCACCGCAGTTAATGCGATCCACGAgg                                                           >  1:810804/1‑236 (MQ=255)
                                                                                                                                                                                          aaaaggtgtccTTTGTGCGTTTTTACGCCCGCAGTGGCGCGGAACATATCCCCGTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGttt                                                   <  1:280322/230‑1 (MQ=255)
                                                                                                                                                                                          aaaaTGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGtt                                                    >  1:535769/1‑238 (MQ=255)
                                                                                                                                                                                                               tttACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGGGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCCCTCTGCGGTGGAAATCTTCCAGCGCCCTACTTTTGTCCGCCCCCCAGTTAATTGCATCCCGCGGGCCCAGCTTTGGTGACAGATTGCCTtcaa                               >  2:562250/1‑237 (MQ=255)
                                                                                                                                                                                                                         cAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCTCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGCTTGAATTCAGTCAGCATCgc                     >  1:201062/1‑238 (MQ=255)
                                                                                                                                                                                                                                    aaCATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGTTAGCCAGCCCTGAATCGCCAGCCCGCTGCCGTGGAAATCTTCAAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCCCCAGGCCGGGTTTCGTGGACAAATTAGCTTCAGTCAGCAGGGCCCGccagcccg          >  2:357076/1‑237 (MQ=255)
                                                                                                                                                                                                                                           cACCTCCGCAGGCCATACCAATGGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGCGCCAGCCCAGCAGGGCg  <  1:898482/239‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             
GGTAAGGCCAAGCTGTTGGTACAACCGTTGACCTGC‑CGTCAGTTGTGAA‑TTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑G‑CCGCCGTAGAACAAACGGTTGTTGGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAC‑ATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGCGCCAGCCCAGCAGGGCG  >  NC_000913/646979‑647447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A