Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F9 I0 R1 260 102.5 2178490 93.2% 2030352 226.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 647,210 A→C 26.2% G100G (GGT→GGG)  citG ← 2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913647,2100AC26.2% 85.9 / 7.2 58G100G (GGT→GGG) citG2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase
Reads supporting (aligned to +/- strand):  ref base A (30/10);  new base C (2/15);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 1.61e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCCGGTAAGGCCAAGCTGTTGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGCGCCAGCCCAGCAGGG  >  NC_000913/646974‑647445
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                           
ggcccGGTAGGGCAAATGTTTTGGTACAACTGTGTCCCTGCCGTAGTTTGGAAATTATTGGTCGGCAGTCGGCGAGGGGTCAGGCCAGGGAAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCAc                                                                                                                                                                                                                                            <  2:1073115/235‑1 (MQ=255)
                gCTGTTGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCCTACCa                                                                                                                                                                                                                           >  1:716778/1‑239 (MQ=255)
                gCTGTTGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCa                                                                                                                                                                                                                           >  1:176203/1‑239 (MQ=255)
                     tGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCCGTGGCGCGGAACAAATCACCTTCGCAAGCCATACCAATTgg                                                                                                                                                                                                                      >  1:1007646/1‑239 (MQ=255)
                     tGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTgg                                                                                                                                                                                                                      >  1:697561/1‑239 (MQ=255)
                     tGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATAACCTTCGCAAGCCATACCAATTgg                                                                                                                                                                                                                      >  1:591756/1‑239 (MQ=255)
                      ggTAAAACCTTTGACCTGCCGTCGGTGGTGAATTTTTGGTCCGCAGTTCGCGATCGGTCAGGCCACGGCAAAAATTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAACCGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAAt                                                                                                                                                                                                                         <  1:279826/235‑1 (MQ=255)
                            cctgtgtcctggCCGTCGTTTGTAAATTATGGTGACGCAGTTGGGGTTCGGTCGGGCCACGGCAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAAt                                                                                                                                                                                                               <  1:1082074/228‑1 (MQ=255)
                              cGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATCGCAGCCCTAAAGAAAAACTCCTGCCTTTATGCGTGTTTACGCCCGCAGGGGCCGGGAACATATCACCTTTCCAAGCCCTACCCATTTGGCGAAAccc                                                                                                                                                                                                             >  2:52817/1‑239 (MQ=255)
                                ttGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGt                                                                                                                                                                                                           >  1:401506/1‑239 (MQ=255)
                                ttGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCACTGGGGCGGAACAAATCACCTTCGCAAGCCATCCCAATTGGGCGGAACCCg                                                                                                                                                                                                            >  2:279826/1‑238 (MQ=255)
                                ttGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGACGCCCTAAAGAAAAAAAGCTGCCTTTATGAGTTTTTAAGCCCCCACTGGCCGGGAAACAAACCCCTTCGCAAGCCCTACCAATTggcgggaagccgg                                                                                                                                                                                                           >  2:717854/1‑228 (MQ=255)
                                 tGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTg                                                                                                                                                                                                          >  1:14685/1‑239 (MQ=255)
                                  gACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTG‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTgg                                                                                                                                                                                                         <  1:127690/239‑1 (MQ=255)
                                       gCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGCAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCCGGTTTACGCCCGCAGTGGCGCGGAACAATTCCCCTTCCCAAGCCATACCAATTGGGCGTAATcctgggggac                                                                                                                                                                                                     >  2:861205/1‑230 (MQ=255)
                                       gCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATTCGTGTTTACGCCCGCAGTGGCACGGAAAATATCACCTTCGCAAGCCCTACCAATTGGGCTGAAACCGTGGAgaa                                                                                                                                                                                                     >  1:441991/1‑236 (MQ=255)
                                                      ttttgtgtccgcgGTTGGCGATGGGGAGGGCCAGGGCA‑AAACTTGGCCGCAGTAGAACAAACGGTTTTT‑GGCGTTCCGGGTTGTTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCAt                                                                                                                                                                                     <  1:318962/230‑1 (MQ=255)
                                                         ttGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCAttt                                                                                                                                                                                   >  1:98749/1‑238 (MQ=255)
                                                                      cgcgATCGGTCAGGCCACGGCAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGGTTTTCCGCCCGCAGGGGCCCGGAACATATCACCTTCGCAAGCCATCCCCATTGGGCGTAATCCGTGGGGTCCCCCTCCTGGTGCCATTTCCCCCCTAAAGGc                                                                                                                                                                      >  2:86620/1‑238 (MQ=255)
                                                                           tgggtggggcccggggagaatttt‑GCCGGCTGAGAAAAAACGGTTGTT‑GGTGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCAAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCaaa                                                                                                                                                                <  2:98749/217‑1 (MQ=255)
                                                                              gTCAGGCCACGGCAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTc                                                                                                                                                             >  1:516768/1‑239 (MQ=255)
                                                                              gTCAGGCCACGGCAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCCCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTACACCGTGGAGTCCCGCTTCTGGTGCCATTTCCGCACCTCAGTCACCAAATTc                                                                                                                                                             >  1:825460/1‑239 (MQ=255)
                                                                                    cccgggaaaaaccTT‑GCCGGCGTAAAAAAAAGGGTGGGG‑GGCGTTCCCGGTGGGTTGAGTGGAAGCAACCGGCCAATTGCCGCACATAGCAGCCCAAAAGAAAAAATGCTGCCTTTAGGCGTGTTTACGCCCGGAGGGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGaa                                                                                                                                                       <  2:716778/227‑1 (MQ=255)
                                                                                     cccgggcaaaatcgtcccggctgaGAACAAACGGTTGTT‑GGCGTTACCGTGTGGTTGGGTTGAAGCAACCGGCCAATTGCCGCACATAGCACCCCTAAAGAAAAAATGCTGCTTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGaa                                                                                                                                                       <  2:313790/216‑1 (MQ=255)
                                                                                     cccGGCAAAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCTTTACCGGTTGGTTGAGTTGAAGAAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGaa                                                                                                                                                       <  1:761182/236‑1 (MQ=255)
                                                                                        ggCAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACgg                                                                                                                                                   >  1:790885/1‑239 (MQ=255)
                                                                                          cAGAAATTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCTTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAAATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACggg                                                                                                                                                  <  1:730872/238‑1 (MQ=255)
                                                                                           aaaaattttcccggcgGAGAACAACTGTTTGTG‑GGGGTTACTGGTTGGTTGAGTGGAAGCAAACGGCCACTTGCCGCACATAGCACCCCTAAAGAAAAAATGCTGCCTTTAGGGGTGGTTCCGCCCGCAGTGGCGGGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACggg                                                                                                                                                  <  2:1052370/228‑1 (MQ=255)
                                                                                             aaaCTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTgg                                                                                                                                                                                                         >  2:626733/1‑180 (MQ=255)
                                                                                             aaaCTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTgg                                                                                                                                                                                                         <  1:626733/180‑1 (MQ=255)
                                                                                             aaaCTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGtg                                                                                                                                               >  1:218488/1‑237 (MQ=255)
                                                                                              aaCGT‑GCCGCCGTAAAAAAAACGGTTGTG‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCAAATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGGGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTagc                                                                                                                                             <  1:197144/239‑1 (MQ=255)
                                                                                                cttttgcctccggaaaaaaaGGGTTGTTGGGCTTTCCCGGGTGGTGATGTTGAACCAACCGGCCAATGCCCGCCCATAGCGGCCCTAAAGAAAAATGGCTGCCTTTATGCGGGTTTCCGCCCGCAGGGGCGCGTAACATACCCCTTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTagc                                                                                                                                             <  2:572482/222‑1 (MQ=255)
                                                                                                     ccgccgTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGAAATCCGTGGAGTACCGCTTCTGGGGCCCTTTCCCACCTACAGGCACCAAATTCAATAAAACGGGGTAACCAGccc                                                                                                                                       >  2:925676/1‑239 (MQ=255)
                                                                                                                     ggttttttgtctttccgggttgtgtGAGTGAAAGCAACCGGCAAAGTGCCCCAAATAGCAGCCCTAAAGAAAAAATGCTGCTTTTCGGGGTTGTTACCCCCGCAGTGGCGCGGACAATTTCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAgcgcg                                                                                                                        <  2:176203/222‑1 (MQ=255)
                                                                                                                             ggCGTTACCGGTTGGTTGAGTTGAAGCAAACCGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGCCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGACCCGCTTCTGGGTCCCTTTCCGCACTCCAGGACCCAAATTCAATGAAACGGGGTAGCCAGccccaaaaacccaagccgctgggggg                                                                                                                >  2:465529/1‑216 (MQ=255)
                                                                                                                                        ttggttgAGTTGAAGCAACCGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTTTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTcc                                                                                                      <  1:306667/238‑1 (MQ=255)
                                                                                                                                         tggtttggttGAACCAACCGGCCATTTGCCGTACAAAGCAGCCCTAAAGAAAAACTGCTGCCTTTATGCTTGTTTCCGCCCGCATGGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCa                                                                                                     <  1:757919/238‑1 (MQ=255)
                                                                                                                                             tgattgaaaGCAACGGGACATTGGCCGCACATGACAGCCATAAAAAAAAAATGCTGCTTTTTGGCGTTTTTCCGCCCGCGGTGGCGGGGATAATATCCCCTGCGAAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGcc                                                                                                <  2:1058094/232‑1 (MQ=255)
                                                                                                                                             tgagttgaAGAAAGCGGCAAATGGCCGCACAGAGCAGCCCAAAAGAAAAACGGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGcc                                                                                                <  1:465529/239‑1 (MQ=255)
                                                                                                                                               agttgaAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTAAAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCat                                                                                              >  1:1033245/1‑239 (MQ=255)
                                                                                                                                                             cccagtcgccgccAATAGCCGCGCTAAAGAAAAATGTCGTCTTTTCGGGGTTTTTACCCCGGCAGTGGCGCGGACGTGATCGCCTCCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCAc                                                                                <  2:497720/232‑1 (MQ=255)
                                                                                                                                                               caatgcccGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGGGGCGCGGAAAATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCAcc                                                                               <  1:659159/232‑1 (MQ=255)
                                                                                                                                                                      cGCACATAGCAGCCATAAAGAAAAAATGCTGCCTTTAGGCGTGTTTCCGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTaa                                                                       <  1:861205/239‑1 (MQ=255)
                                                                                                                                                                        cacaTAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCCCCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATAGCCCACCCCGTCGGCGGGAAAACTTCCACCGCCATACCTTGTTGCGCACCCCAGTGaaag                                                                     >  2:193197/1‑237 (MQ=255)
                                                                                                                                                                               cagcCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAACGCGCCGCGGGGGAAATCTTCCAGCGCCATATCTTTGTGCCCCCCGCAGTTAAtcggaccc                                                               >  2:83645/1‑231 (MQ=255)
                                                                                                                                                                                 gcCCTAAAGAAAAAATGTTGCCTTGAGGCGTGTTTACCCCCGAGGGGGCGGGGAACTTTCCCCCTTGGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACg                                                            <  1:889892/239‑1 (MQ=255)
                                                                                                                                                                                   ccTAAAGAAAAAAGGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAGTGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCAc                                                             <  1:845240/236‑1 (MQ=255)
                                                                                                                                                                                   caaaaaGAAAAAATGCTGCCTTTAGGCGGTTTTACGCCCGCAGTGGCGCGGAACATATCCCCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACg                                                            <  1:86620/234‑1 (MQ=255)
                                                                                                                                                                                       aaGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATCCGATCCACGAgg                                                         >  1:904358/1‑236 (MQ=255)
                                                                                                                                                                                                      tttttgtcGTTTTTCCCCCCGGCGTGGCGCGGAACATTTCCCTTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGa                                       <  2:1033245/232‑1 (MQ=255)
                                                                                                                                                                                                               tgtTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCCTATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGATTCGGTGCAAGATTGGCTtca                              >  1:860598/1‑239 (MQ=255)
                                                                                                                                                                                                               tgtTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTtca                              >  1:908414/1‑239 (MQ=255)
                                                                                                                                                                                                               tgtTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTtc                               >  1:264924/1‑238 (MQ=255)
                                                                                                                                                                                                               tgtTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAACCGTCCACAGCCATATCTTTGTGCCCACCCCAGTTAAGTCGCTCCCCGAGCCCTGGTTCCGGTGACAGAtgtacgtca                              >  1:649825/1‑231 (MQ=255)
                                                                                                                                                                                                                 tttACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCAGATCTTTGTGCGACCCCCAGTTAATGCGATCCACGGGGCCAGGTTTCGGTGAAAGAATGACCtcgga                            >  2:160168/1‑236 (MQ=255)
                                                                                                                                                                                                                                gcgcGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGCGGCCTGGt                                                   >  1:129623/1‑201 (MQ=255)
                                                                                                                                                                                                                                           atCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACAAGGCCTGGTTTCGGTGACAGATTGCCTTCAGTCAGCTTTGCGCGCCAGCCCAGCCggg  >  1:862440/1‑239 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                           
GCGCCGGTAAGGCCAAGCTGTTGGTACAACCGTTGACCTGCCGTCAGTTGTGAATTATTGGTACGCAGTTCGCGATCGGTCAGGCCACGGCAGAAACTT‑GCCGCCGTAGAACAAACGGTTGTT‑GGCGTTACCGGTTGGTTGAGTTGAAGCAAACGGCCAATTGCCGCACATAGCAGCCCTAAAGAAAAAATGCTGCCTTTATGCGTGTTTACGCCCGCAGTGGCGCGGAACATATCACCTTCGCAAGCCATACCAATTGGGCGTAATCCGTGGAGTACCGCTTCTGGTGCCATTTCCGCACTACAGGCACCAAATTCAATGAAACGGGGTAGCCAGCCCTGAATCGCCAGCGCGCTGCGGTGGAAATCTTCCAGCGCCATATCTTTGTGCGCACCGCAGTTAATGCGATCCACGAGGCCTGGTTTCGGTGACAGATTGACTTCAGTCAGCATGGCGCGCCAGCCCAGCAGGG  >  NC_000913/646974‑647445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A