Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F18 I0 R1 274 115.5 2655328 86.7% 2302169 226.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,158,652 T→G 11.0% V55G (GTG→GGG)  oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,158,6520TG11.0% 296.8 / 4.3 145V55G (GTG→GGG) oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (54/65);  new base G (14/5);  total (75/70)
Fisher's exact test for biased strand distribution p-value = 2.67e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

CTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGG  >  NC_000913/4158418‑4158888
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             
cTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGATTATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTATCCAGCCGAAGCTTAGCGGGCAAATCCGTAAGCTGGAAACTGAGCTGGGCGTGATGTTGCGGGAGCGGACAA‑GCCGTAACGggttg                                                                                                                                                                                                                                           >  2:1237630/1‑239 (MQ=255)
  tGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGTTGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑CCCGTAAGGtgttgt                                                                                                                                                                                                                                          >  2:185791/1‑238 (MQ=255)
  tGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTgttgtt                                                                                                                                                                                                                                         >  2:391753/1‑239 (MQ=255)
      aGGGATAATCTTTCATTGCTATTCTGCCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGATTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCAcc                                                                                                                                                                                                                                     <  2:1136664/239‑1 (MQ=255)
        ggATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGGGCGGACCA‑GCCGTAAAGGGTTGTTCACCCa                                                                                                                                                                                                                                   >  2:918323/1‑239 (MQ=255)
          aTAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAgg                                                                                                                                                                                                                                 >  2:214709/1‑239 (MQ=255)
          aTAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCCGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAgggtctgtcccttata                                                                                                                                                                                                                                 >  2:523732/1‑227 (MQ=255)
           tAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATCATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGAGGTTGCTGGGGCGGACCA‑GCCGTAAAGGGTTGTTAACCCAGGc                                                                                                                                                                                                                                >  2:605211/1‑239 (MQ=255)
                   cATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTTGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTAACCCAGGCGGGAAtgc                                                                                                                                                                                                                        >  2:79573/1‑239 (MQ=255)
                   cATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGATGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCGGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCGGAAAAGTGTTGTTCACCCAGGCGGGAAtgc                                                                                                                                                                                                                        >  2:542001/1‑239 (MQ=255)
                      tGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGGAtgcg                                                                                                                                                                                                                       >  2:159753/1‑236 (MQ=255)
                        cTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGCGTTGTTCACCCAGGCGGGAATGctgctg                                                                                                                                                                                                                   >  2:919707/1‑239 (MQ=255)
                           ttCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCtgggg                                                                                                                                                                                                                >  2:875935/1‑237 (MQ=255)
                              tACCTATCGCCATGAACTACCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTGGAGTCCCGGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGAGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGAt                                                                                                                                                                                                             <  2:120372/239‑1 (MQ=255)
                                ccTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGATTATTCTTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGTCAGATTCCT‑GCCACGTTAGCCAGCAGACGCTTAGCGGGCCATTCAGACACCGGGAAGATGAGCTGGGCGTGATGTTGCTGTATCGGACCA‑GCCGACAAGAG‑TGTTTACACAAGCggggaactctcctgggggagcg                                                                                                                                                                                                          >  1:927345/1‑220 (MQ=255)
                                ccTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGACTATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGTTGGAAGATGTCCTGGGCGCGAAGTGCATGGCGGGGATCACGCGGAAAGGTGTT‑TTCACCCACGCggggaactgtcggggggacag                                                                                                                                                                                                           >  1:1077672/1‑221 (MQ=255)
                                ccTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTATCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGGGATGTTGCTGGCGCGGACCA‑GCCGTAAAGTGTTGTTCACCCCGGGGGGAATGGTGCtgggggggca                                                                                                                                                                                                           >  1:1197747/1‑235 (MQ=255)
                                ccTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCGGGACGGGACCA‑CCCGTAAAGTGTTGTTACCCCAGGGGGGAAtgggggggggggagag                                                                                                                                                                                                           >  1:697810/1‑225 (MQ=255)
                                   aTCGCCATGACCTATCGGGGCGATGGAGGATGGATAAGGAATATTGGTGATCGTGGTTACCGGGTGG‑CATTGGCTGACCC‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGgc                                                                                                                                                                                                        <  2:833139/239‑1 (MQ=255)
                                   aTCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCTTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGCCCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCGTGTGGATCAGgc                                                                                                                                                                                                        >  2:731635/1‑239 (MQ=255)
                                   aTCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAAtg                                                                                                                                                                                                                         >  2:138521/1‑222 (MQ=255)
                                   aTCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAggg                                                                                                                                                                                                        >  2:305047/1‑238 (MQ=255)
                                     cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGgcgc                                                                                                                                                                                                      >  2:120618/1‑239 (MQ=255)
                                     cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACATGGTGG‑CATTGGCTGAACA‑CCGCCATTTTAGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCCACTTCGTAAGCTGGACGATGCGCTGGGCGTGATTTTTCTGTGTGGGCCCA‑GGCGGAACATGTTGTTCACCCAGGGGGGGAtgagggggggaggacgagggg                                                                                                                                                                                                      >  1:1119812/1‑220 (MQ=255)
                                        cagaaactaggggggggagggagggTGGAAAATGAAATTGCGGGACTTGGGGTCCCGGGGGG‑CATGGGGT‑AACACCCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTa                                                                                                                                                                                                   <  2:1296113/220‑1 (MQ=255)
                                                 tCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTCCTGGTGGATCAGGCGCGTACCGtgctgc                                                                                                                                                                                          >  2:1016067/1‑239 (MQ=255)
                                                 tCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGCACCTGGTGG‑CCTTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGGGCGGGGCGTGATGTTCCTGGAGCGGACCA‑GCCGGAAAGTGTTGTTCCCCCAGGTGGGAATGCTGCTGGTGGATCAGGGCTGGACCGggctgc                                                                                                                                                                                          >  1:997115/1‑239 (MQ=255)
                                                  cGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGCTTGTGCTGGACCGGAACA‑CCCGTAAAGTGTTGTTCAACCCAGCGGGAGTTGTGCTGGTGGATCAgggccgacaccacccgcc                                                                                                                                                                                         >  1:744959/1‑223 (MQ=255)
                                                      gCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGGTGAGCTGGGCGGGATGTTTCTGGACCGGACCA‑GCCGTACAGAGTTGTTCCCCCAGGCGGGAATGGTGCGGGTGGATCAGGCGCGTAcctgccgcggggag                                                                                                                                                                                     >  1:435809/1‑227 (MQ=255)
                                                      gCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGGGGATCAGGCGCGTACCGGGCTGCGTGAg                                                                                                                                                                                     >  2:818581/1‑239 (MQ=255)
                                                      gCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGGGTTGTTCACCCAGGCGGGGGTGCGGCTGGTGGATCAGGGGCGTACCGTGCTGCGTGAg                                                                                                                                                                                     >  2:157110/1‑239 (MQ=255)
                                                            ggtgatggatAATTAATTTTCGTGAT‑TTGGGGTCCTGGGGGTCTTGGGCTGACCC‑CCGCCATTTCCGGGGGGCGGCAGATGCCT‑GCCACGTTGGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGTGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGaa                                                                                                                                                                                <  1:1016067/235‑1 (MQ=255)
                                                              ggatgtatAATGAATTTTCGGGATTGTGGGTCCCGGGTGG‑CATGGGCTGAACC‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAg                                                                                                                                                                              <  2:1168403/238‑1 (MQ=255)
                                                              ggatggatAATGAATATTCGTTAGCTGGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGGGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAg                                                                                                                                                                              <  2:173287/238‑1 (MQ=255)
                                                                    atAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGATGGACGATGCGCTGGGCGTGATGTTTCTGGAGCGGACCG‑TCCGTCAAGGGTTAGTTACCCAGCCGGGAGTGGTGTCGTGGGAGCGGACGCGTACGGTTCCGCGGGGGGGGAAAGGCCTT‑aa                                                                                                                                                                      >  1:888480/1‑239 (MQ=255)
                                                                       aTGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTTATGTTGCTGCAGCGGCCCA‑GCCGTAAAGGGTTGTTCACCCAGGCGGGAACGCCCCTGGGTGCTCAGGCGCCGAccgcgccgcgggggggggaaggcccaaagaa                                                                                                                                                                    >  1:1102786/1‑218 (MQ=255)
                                                                       aTGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGGGAGGTGAAAGTCCTT‑AAaga                                                                                                                                                                   >  2:261571/1‑239 (MQ=255)
                                                                        tGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCAGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCGGCTGGGGGATCTGGCGCGAACCGTGCGTCGTGGGGTGAAAGTCCTT‑AAaaag                                                                                                                                                                  >  2:610250/1‑239 (MQ=255)
                                                                               tCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGCCGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGCGGTTGCTGGAGCGGACCA‑GCCGTAAAGAGTTGTTCACACAGGCGGGAATGCTGCTGGTGGAACCGGCGCGGACCGTTGCGCGTGAGGTGAAAGTTCTT‑aaaaaagaggcaa                                                                                                                                                           >  1:677046/1‑229 (MQ=255)
                                                                               tCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGTGGGATCAGGCGCGTACCGTGCTGCGTGAGGGGAAAGTCCTT‑AAAGAGATGGCa                                                                                                                                                            >  2:1150512/1‑238 (MQ=255)
                                                                                    atgttGAGTACCGGGGGG‑CTTGGGCTAAACC‑CCGCCATTTTCGGCGTGCGGAAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCcag                                                                                                                                                      <  2:397953/236‑1 (MQ=255)
                                                                                          aGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGc                                                                                                                                                >  2:1202708/1‑239 (MQ=255)
                                                                                          aGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGGGTTGTTCACCCAGGCGGGGATGCTGCTGGGGGATCAGCGGCGGTCCGTGCCGCGGGGGGGGAAAGGCCTTAAAAGAAAT‑GCAAGCCAGCAgggg                                                                                                                                                >  1:114405/1‑238 (MQ=255)
                                                                                               cTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGGGGTGAAAGGCCTT‑AAAGAGATGGCAAGccctgtttcttttc                                                                                                                                            >  2:997562/1‑226 (MQ=255)
                                                                                                 ttgtgggctgtggggcacccccGCCTTTTGCGGGGTGGGGAAGCTCCCT‑GCCCCGTTGGCCGGCCGACGTTGAGCGGGCAAATCCGTAAGCTGGAAGTGTAGCGGGGCGGGATGTTGCTGGGGGGGACCA‑GCCGGAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGAc                                                                                                                                           <  1:1149533/219‑1 (MQ=255)
                                                                                                 ggtgg‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCCGGCGCGTACCCTGCTGCGTTGGGGGCAAGCCCTT‑AAAGAGATGGCAAGCCCGCCGGGCGAGACGa                                                                                                                                         >  2:189238/1‑239 (MQ=255)
                                                                                                 ggtgg‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGGGCTGCGGGAGGGGAAAGTCCTT‑TAAGAGATGGCAAGCCAGCAGGGCggacgga                                                                                                                                         >  2:351246/1‑233 (MQ=255)
                                                                                                       cATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGGGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGAAGGGGGGGACGAttgcc                                                                                                                                    >  2:694597/1‑235 (MQ=255)
                                                                                                        tttGGCTGACCA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCg                                                                                                                                   <  2:8514/238‑1 (MQ=255)
                                                                                                         ttGTCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGCTGTTGCTGGAGCGGACCA‑GCCGCAAAGTGTTGTTCACCCAGCCGGGAATGTGCCTGGGGGATCAGTAGAGTACCGCGCTCCGTGCGGGGAAACACCTT‑AAAAGGAGGGCAAGCCAGACGGGACAGACgcagacacg                                                                                                                                  >  1:996898/1‑231 (MQ=255)
                                                                                                                     tcccctttttGGGGGTGGGGCATGTTCCT‑GCCAGGTTGGCCCGCCGACGCTTAGCGGGCAAATTCGGAAGCTGGAAGGTGGGCGGGGCGTGGTGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGcaca                                                                                                                       <  1:334729/233‑1 (MQ=255)
                                                                                                                     ccGCCATTTGCGGCGGGCGGCAGATTCCT‑GCTAAGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGcaca                                                                                                                       <  2:1119812/239‑1 (MQ=255)
                                                                                                                        ccATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGGGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGGGACGATGTCCGGGCCGCTGCACAt                                                                                                                      >  2:1237135/1‑237 (MQ=255)
                                                                                                                                   ggggggcggtttCCT‑GCCAGGTTGGCCAGCGGAGCCTTAGCGGGAAATTTCGAAAGCGGAAAGATGGGCGGGGGGTGATGTTGCTGGGGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTcc                                                                                                          <  1:84055/228‑1 (MQ=255)
                                                                                                                                   ggggggCAGTTTCCG‑TCCCCTTTGGCCGGCCGGCGCTGAGGGGGAAAATTCGTAAGCTGGAAGATGAGTGGGGGGGGAGTTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTcc                                                                                                          <  1:856846/234‑1 (MQ=255)
                                                                                                                                   gggcggggagttCCT‑GCGCGGTTGGCCGGCCGACGTGTGGGGGGAAAAGTGGTAAGCTGGAAGAGGAGCTGGGCGGGGTGGTGAGTGAGCGGACCA‑GCGGTAAAGTGTTGTTCCCCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGGACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTcc                                                                                                          <  1:918323/228‑1 (MQ=255)
                                                                                                                                   ggcaggtaggttCCT‑GGCAGGTTAGCCAGCCGAGGCTTAGCGGGCAAATTCGTAAGCTGGAAGAGGAGCTGGGCTTGATGTTGCTGGAGCGGACCA‑GGCGTAAAGTGTTGTTCACCCAGGTGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTcc                                                                                                          <  2:997115/228‑1 (MQ=255)
                                                                                                                                   gTGCGGCAGTTTCCT‑GCCAGGTTTGCCGGCGGAGGCTTGGCGGGAAAATGCGTAAGGTGGAAGGTGGGCTGGGGGTGATGTTGCTGGAGCGGCCCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTcc                                                                                                          <  1:145196/238‑1 (MQ=255)
                                                                                                                                   gTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTcc                                                                                                          <  2:888480/238‑1 (MQ=255)
                                                                                                                                    ggcggacgATTCCTCGCCGCGTTGCCCCTCCGTCGCTTAGGGGGAAAAGCCGGAGGCGGGAAGAGGTGCTTGGCGTGATGTTGGTGGAGCGGACCA‑GCCTTAAAGTGTTGTTCCACCAGGCGGCAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTcc                                                                                                          <  1:605211/231‑1 (MQ=255)
                                                                                                                                      cGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGGGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCCCATTGGGTTGATTCCccca                                                                                                      >  2:324130/1‑239 (MQ=255)
                                                                                                                                        gCAGATTCCT‑GCCACGTTAGCCAGCCGGCGCTTAGCGGGCCATTTCGTAAGCTGGAAGAGGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAg                                                                                                     <  2:350099/238‑1 (MQ=255)
                                                                                                                                             ttCCG‑GCCAATTTAGCCAGCCGGCGGTTAGCGGGGAAATTCGTAAGGTGGAAGATGAGCTGGGCGTGATGTTGCGGGAGCGGACCA‑GCCGTAAAGTGTTGGTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGAc                                                                                               <  2:744959/239‑1 (MQ=255)
                                                                                                                                                 t‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGGTGTCCGGCCCGCTGCCCATTGGTTTGATTCCCCCAGTTGGACCGt                                                                                            >  2:806450/1‑238 (MQ=255)
                                                                                                                                                  ggCCACGTTGGCCAGCCGACGGTTAGCGGGCAAATGCGTAAGCTGGAATATGGGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTa                                                                                           <  2:677046/238‑1 (MQ=255)
                                                                                                                                                      aCGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTg                                                                                       <  2:654681/239‑1 (MQ=255)
                                                                                                                                                       cGTTGGCCGGCCGACGTTTGCGGGGAAAATCCGTAAGTGGGAAGAGGGGTTGGGCGTGATGGTGCGGGAGCGGACCA‑GCCGGAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGc                                                                                      <  1:723154/239‑1 (MQ=255)
                                                                                                                                                       cGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGACCAGGCTCGTACCGTGCGGCGGTAGGGGAAAGTCCTT‑AAAGAGAGGGCAAGGCACCAGGGCGGGGCGAGGTCCGGACACCGCCACATGGGGTTAATTCCCACAGTTGGAcagagcctg                                                                                       >  1:279330/1‑230 (MQ=255)
                                                                                                                                                         ttAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGCTGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGGACCTGCt                                                                                     >  2:1036542/1‑238 (MQ=255)
                                                                                                                                                           tgccgggcggcggttggCGGGCAAATGGGTAAGGTGGAAGAGGAGGTGGGCGGGCTGTGGCTGGAGGGGACCA‑GCCGTAAATGGTTGGTCACCCAGGCGGGCATGCTGCTGGTGGATCAGGCGCGTACCGTGCCGCGTGAGGTGAAAGTCCTT‑ACAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTAcc                                                                                  <  1:1016533/223‑1 (MQ=255)
                                                                                                                                                           agccagccTACGTTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTAcc                                                                                  <  2:1093667/239‑1 (MQ=255)
                                                                                                                                                                ggcggtgttttgggggAAAATTCGTAAGCTGGAAGATGAGGTGGGCGGGATTTTGCTGGAGCGGACCA‑GCCGTAAAGGTTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCata                                                                             <  1:1024283/226‑1 (MQ=255)
                                                                                                                                                                  cggcGCTTAGCGGGCAAATTCGTAAGGTGGAGAGTGAGCTGGGAGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCatat                                                                            <  2:554358/235‑1 (MQ=255)
                                                                                                                                                                  cGACGCTTAGGGGGAAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCatat                                                                            <  2:350613/238‑1 (MQ=255)
                                                                                                                                                                  cGACGCTTAGCGGGCAAATTCGGAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCatat                                                                            <  2:1102786/238‑1 (MQ=255)
                                                                                                                                                                      cttgagggggAAAATTCGTAAGTGGGAGGTGGAGCGGGGCGTGGTGTGGGTGGTGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGTAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATcc                                                                       <  1:261571/232‑1 (MQ=255)
                                                                                                                                                                           gCGGGCAAATTCGTAAGCGGGAAGATGAGCTGGGCGTGATGTTGCGGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtg                                                                  <  2:1186153/239‑1 (MQ=255)
                                                                                                                                                                               gCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGGACCGTGCCGCGTGAGGGGAAAGTCCTT‑AAAAAAATGGCAAGCCAGCAGGGCGCGACGACGTCCGGGCCGCTGCACCTTGGGTTGATTTCCACAGTGGGACCGTACCTCCAACCCACTCTTATccccaagccc                                                               >  1:1178614/1‑231 (MQ=255)
                                                                                                                                                                                cAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGGGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCgaga                                                                                                                                            >  2:431228/1‑160 (MQ=255)
                                                                                                                                                                                              ggAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGGCCTTTCCaa                                               >  2:1031861/1‑239 (MQ=255)
                                                                                                                                                                                              gaacgggtggCTGGTGGTGGTGTTGCGGGGGGCGCCCA‑GCCGTAAAGTGGTGTTCCGCCAGGCGGGAATTGTGCGGGGGGATCAGGGGCGTACCGTGCTGCGGTATGTGAAAGTCCTT‑AAAGAGATGGCTAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATGCCCACAGTTGGACCGTACCTGCTACCGCAGATTATCCCTATGCTGCAGCAGACCTTTCCaa                                               <  1:324130/230‑1 (MQ=255)
                                                                                                                                                                                                     gggttgggggtggtttggggggAGGGGCCCG‑GGCGTAAAGTGTTGGTGACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTgg                                         <  1:51106/218‑1 (MQ=255)
                                                                                                                                                                                                      aGCTGGGCGTGATGTTGCTGGAACGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCGGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGGCCTT‑AAAGAGATGGCAACCCAGCGGGGCGAGACGATGTCCGGACCCATGCACATTGGGTTGATTCCCCCAGTGTGACCCTACGTGCGACCGCCAAGTTGCCCCAGGTCGCACCATACCTTTCCaaaacacaaa                                       >  1:744253/1‑232 (MQ=255)
                                                                                                                                                                                                          gggCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGt                                   >  2:77034/1‑239 (MQ=255)
                                                                                                                                                                                                           ggCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGGGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGGCCGGACCGCTGCCCATTGGTTTGATTCCCACAGTTGGAAGGAACCTGCTCCCGCCCATTATCCCTATGCTTAACCAGACCCTTCCAAAACTGGaaaagga                                  >  1:654894/1‑235 (MQ=255)
                                                                                                                                                                                                             cgggATGTGGCGGGAGCGGACCA‑GCCGTAAAGGGTTGTTCACCCAGGCGGGAATGCTGGTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTAt                                 <  2:516881/235‑1 (MQ=255)
                                                                                                                                                                                                             cGTGAGGTTGCTGGAGCGGACCA‑GCCGTAAAGTGGTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTAt                                 <  2:1096993/238‑1 (MQ=255)
                                                                                                                                                                                                                   gTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGgc                                                                                                                                                                                                        <  2:364588/66‑1 (MQ=255)
                                                                                                                                                                                                                   gTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATg                          <  2:850623/239‑1 (MQ=255)
                                                                                                                                                                                                                     tGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTcc                                                                                                          <  1:815930/157‑1 (MQ=255)
                                                                                                                                                                                                                       cTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGGTGGACCGTACCTGCTCCCGCCAATTATCCCTATTCTGCACCAGACCTTTCCAAAGCCGGAAAGGTAACTTCATGAAGc                      >  1:1043866/1‑239 (MQ=255)
                                                                                                                                                                                                                         ggAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCCCAGTTGGACCGTACCTGCTACCGCATCTTATCCCTATGCTGCACCAGAc                                                       >  2:712941/1‑204 (MQ=255)
                                                                                                                                                                                                                           aGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGcaca                   >  2:1019407/1‑238 (MQ=255)
                                                                                                                                                                                                                             cGGACAA‑GCAGAAAAGTGTTGTTCACCCAGGCGGAAATGCTGCTGGTGGATCAGGCGCGTACCGTGATGGTTGAGGTTAAAGTCCTT‑AAAGGGATGGCAAGCCAGCAGGGCGAGGCGATGTCCTGACCGCTGCACATTGGTTTGATTCCCACAGGTGGACCGTGCCTGCGACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCTCAgt                 <  2:155568/238‑2 (MQ=255)
                                                                                                                                                                                                                               gACCA‑GCCGTAAAGTGTTGTTCCCCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGAcc               <  2:1121963/238‑1 (MQ=255)
                                                                                                                                                                                                                                 ccA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACcca             <  2:697810/238‑1 (MQ=255)
                                                                                                                                                                                                                                  ccgcctgTAAATTGTTTTTCCCCCGGGCGGGAAGGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGGGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACccac            <  1:391753/233‑1 (MQ=255)
                                                                                                                                                                                                                                  cccggcGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTG‑AAAGAAATGGCAAGCCAGCAGGGCGAGACGATGGCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTGCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACcca             <  1:189238/233‑1 (MQ=255)
                                                                                                                                                                                                                                            aGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTgg  <  2:1178614/239‑1 (MQ=255)
                                                                                                                                                                                                                                            aGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTgg  <  2:435809/239‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             
CTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGG‑CATTGGCTGAACA‑CCGCCATTTTCGGCGTGCGGCAGATTCCT‑GCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCA‑GCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTT‑AAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGG  >  NC_000913/4158418‑4158888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A