Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 322 127.1 2757226 91.8% 2531133 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,158,652 T→G 14.7% V55G (GTG→GGG)  oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,158,6520TG14.7% 248.0 / 10.0 124V55G (GTG→GGG) oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (41/58);  new base G (17/4);  total (62/62)
Fisher's exact test for biased strand distribution p-value = 1.38e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 128 total aligned reads displayed.

AATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGG  >  NC_000913/4158414‑4158888
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              
aaTGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGAGGTTGCTGGAGCGGACCAGCCGTAAAgt                                                                                                                                                                                                                                                >  1:462713/1‑239 (MQ=255)
      tGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGtgttgt                                                                                                                                                                                                                                           >  1:958317/1‑238 (MQ=255)
              aTAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGTTGGGCGGGATGTTGCTGGGGGGGAACAGCCGGAAAGGGTGGTTccgcccgg                                                                                                                                                                                                                                  >  2:1235307/1‑232 (MQ=255)
               tattctttctttttttttttACCTTTCGCCATGAACTTGGGGGGGGGGGGGGGATGGAAAAGGAATTCTCGGGATGTG‑GAGTCCGGGGTGGCATTGGC‑GGAA‑CCCCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGc                                                                                                                                                                                                                                 <  2:1256288/220‑1 (MQ=255)
               tAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAACAGCCGTAACGAGGTGTTCACCCAGGc                                                                                                                                                                                                                                 >  1:549588/1‑239 (MQ=255)
                aaTCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGGGCTGGGCGTGATGTTGCTGGACGGGACCAGCCGTAAAGTGTTGTTGACCCaaggg                                                                                                                                                                                                                                >  2:953246/1‑235 (MQ=255)
                       cATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑CCTTACCGGGCAAATTCGTAAGCTTGAAAATGAGCTGGGCGGGATGTTGCTGGTGGGGACCAGCCGAAAAGAGTGTTTCACCCAGGCGGGaaagg                                                                                                                                                                                                                         >  2:714261/1‑236 (MQ=255)
                          tGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAAATTCCTGCCACGTTAGCCAGCCCCC‑GCTTAGCGGGCAAATTCGTAAGCTTGAAGAGTAGCTGGGCGTGACGTTCCTGGAGCGGACCAGCCGTAACGTGTTGTTCCCACAGGGGGGCAtcttgc                                                                                                                                                                                                                      >  2:263420/1‑234 (MQ=255)
                                    ccTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTTCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAACCTGGAAGTTGAGCTGGGCGGGCTGTTGCGGGAGCTGACCCGCCGTAAAGTGTTGTTCACCCAGGCGGGaaaggcggctgggggaca                                                                                                                                                                                                            >  2:650147/1‑223 (MQ=255)
                                    ccTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGTTCa                                                                                                                                                                                                            >  1:709849/1‑239 (MQ=255)
                                    ccTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGGGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCAAGGCGGGAATGCTGCGGGTGGagta                                                                                                                                                                                                            >  1:344083/1‑236 (MQ=255)
                                    ccTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAACTGGGCGGGATGTTGCTGGAGCGGACCAGCCGTAAAGGGTTGTTCACCCAGGCGGGAATTCTGCTGGTGGGTCa                                                                                                                                                                                                            >  1:1266716/1‑239 (MQ=255)
                                       aTCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGAGGTTGCTGGAGCGGCCCACCCGTAAAGGGTTGTTCACCCAGGCGGGAAAGTTGGTGGGGGaaaaggg                                                                                                                                                                                                         >  2:1260617/1‑233 (MQ=255)
                                         cgcgatgacttatggggggggtGGAGGATGTATAATAAAT‑TTGCTGATTTTGGGGTACCTGG‑GGCATTGGG‑TGAACCCCCGCCTTTTTCGGCGTCGGGCGGATCCCTGCCCCGTTAGCCAGCCGGC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGgcgc                                                                                                                                                                                                       <  2:450172/218‑1 (MQ=255)
                                         cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGgcgc                                                                                                                                                                                                       >  1:1060235/1‑239 (MQ=255)
                                         cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGACGGGAATGCTGCTGGTGGGTCAGgtgc                                                                                                                                                                                                       >  1:500580/1‑239 (MQ=255)
                                         cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGGGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGACCAgaggc                                                                                                                                                                                                       >  1:106157/1‑235 (MQ=255)
                                         cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGGGTTGTTCACCCAGGCGGGAATGCTGCTGGGGGATCAGgcgc                                                                                                                                                                                                       >  1:1147969/1‑239 (MQ=255)
                                         cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGGGTTGTTCACCCAGGCGGGAATGCGGCTGGGGGATCAGgcgc                                                                                                                                                                                                       >  1:450172/1‑239 (MQ=255)
                                              tGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGGGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTAcc                                                                                                                                                                                                 >  1:260027/1‑239 (MQ=255)
                                                      cGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGGGTTGTTCACCCGGGCGGGAATGTGGCTGGGGGGTCAgggggggacagggtcggg                                                                                                                                                                                          >  2:232916/1‑223 (MQ=255)
                                                          gCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGATCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTTGGGGAT‑AAGCGCGGTACCGGGCGGCGTAAg                                                                                                                                                                                     >  1:229670/1‑239 (MQ=255)
                                                          gCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGGGGATCAGCCGC‑GTACCGTGCTCCGTggg                                                                                                                                                                                     >  1:1146359/1‑237 (MQ=255)
                                                          gCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTACTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGACGTAAAGTGTTTTTCACCCAGGCGGGAACGGTGGTGGGGGATCAGGCGG‑GTACCGGgcggcggggg                                                                                                                                                                                     >  2:561504/1‑235 (MQ=255)
                                                             aTGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGACATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGGGGATCAGGCGC‑GTACCGTGCTGCGTgggggg                                                                                                                                                                                  >  1:236971/1‑237 (MQ=255)
                                                               ggcggatggatAATGAATTTTGGTGAATTT‑GGTTACCTGGTGCCTTTGGT‑TGAA‑CCCCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGa                                                                                                                                                                                 <  1:540147/235‑1 (MQ=255)
                                                               ggaggtTGGATAAGGAATATTGGTGACCTG‑GGTCACCTGGTGGCATGGGC‑TGAA‑CCCCGCCATTTTCGGCGTGCGGCAGATTCCTGCCAGGTTAGCCAGCCGGC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGaa                                                                                                                                                                                <  1:602038/239‑1 (MQ=255)
                                                               ggaggaTGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGCTGTTGCTGGAGCGGACCAACCGCAAAGTGTTGTTCACCCCGGCGGGACAGCTGCGTGTGGATCCAGCGC‑GAACCGGGCGGCGGGAGGGGaa                                                                                                                                                                                >  2:425628/1‑239 (MQ=255)
                                                                       gaaaaTGAATTTTCGTGATCTT‑GATGCCCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCtt                                                                                                                                                                         <  1:232916/235‑1 (MQ=255)
                                                                        atAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGGTGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACACAGGCGGGAATTCTGATGGTGGGACAGGAGC‑GATACGTGCTGGGGCAGGGGAAAGGCCTTaa                                                                                                                                                                       >  2:94512/1‑239 (MQ=255)
                                                                        atAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGGGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGGGGTGAAAGGCCTTaa                                                                                                                                                                       >  1:1301114/1‑239 (MQ=255)
                                                                        atAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGGGTTGTTCACCCAGGCGGGAAGGGTGCTGGGGGACCAGGCGC‑GTACCGGGCTCGGGGGGGGGAAAGGCCTTaa                                                                                                                                                                       >  2:862619/1‑239 (MQ=255)
                                                                        atAATGAATATTCGTGATCTT‑GAGCACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGAACAGGGGC‑GGACCGGGCTTCGGAGGGGGAAAAGCCTTaa                                                                                                                                                                       >  2:1020313/1‑239 (MQ=255)
                                                                           ttgatttttCGTGTTTTT‑GGGT‑CCTGGGGGCAGTGGCGTGAC‑CCCCCCCTTTTCCGGCGGGCGGCAAATTCCTGCACCGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAaga                                                                                                                                                                    <  2:1168146/232‑1 (MQ=255)
                                                                           aTGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGGGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGGGCTGCGGGAGGGGGAAGTCCTTAAaga                                                                                                                                                                    >  1:569193/1‑239 (MQ=255)
                                                                             gAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGGGATGTTGCTGGAGCGGACAAACCGTAAAGGGTTGTTCACCCAGGCGGGAATACTGCTGGGGGAACAGGCGC‑GGACCGGGCTGGGGGGGGGGGAAGTCCCTaaaaaaa                                                                                                                                                                  >  2:421255/1‑235 (MQ=255)
                                                                               atatTCGGGATCTG‑GAGTACCTGGTGGCATGGGC‑GAAC‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGAt                                                                                                                                                                 <  1:412617/238‑1 (MQ=255)
                                                                                 attccgggttttggggTACCTGTGGGCATTGGC‑TGAA‑CACCGCCATTTTGGGCGTGCGGCAGATTCCTGCCAAGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATgg                                                                                                                                                               <  1:1180043/224‑1 (MQ=255)
                                                                                 atTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGGGGGTCAGGCGC‑GTACCGGGCTGCGTGAGGGGAAAGACCTTAAagaaaggga                                                                                                                                                              >  2:435841/1‑233 (MQ=255)
                                                                                                ggtctttggggcctggggtgaaa‑CCCCGCCTTTTTGGGGGGGGGGCAGTGTCCTGCCACGTTAGCCAGCCGAG‑CCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCGGGGCGGGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGc                                                                                                                                                 <  2:236971/218‑1 (MQ=255)
                                                                                                   ccTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGGGGATCAGGCGC‑GTACCGGGCTCCGGGAGGGGAAAGTCCTTAAAGAGAAGGCAAACCAGCAggggggga                                                                                                                                             >  2:67180/1‑234 (MQ=255)
                                                                                                     tggtggCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTCAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GGACCGTGCTGCGGGCGGGGAAAAGCCTTAAAGGGATGGAAAGCCACCCGGGGGGGACg                                                                                                                                           >  2:798855/1‑239 (MQ=255)
                                                                                                      ggtggCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTCACGTGCTGCGTGAGGGGAAGGGCCTTAAAGAGATGGCAAGCCAGCAGGGCGGGACGa                                                                                                                                          >  1:554481/1‑239 (MQ=255)
                                                                                                      ggtggCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GCACCGGGCGTGGTGAGGGGAAAGGCCTTAAAAGGAGGGCAACCCAGCAGGGCGcgacga                                                                                                                                          >  2:363974/1‑239 (MQ=255)
                                                                                                      ggtggCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAAGCGGCTGGGGGATCAGGGGC‑GTACCGGTCGGCGTGAGGGGGAAGGCCTTAAAGAGATGGCAAGCCAGCAGGGGgaggaga                                                                                                                                          >  2:1056553/1‑235 (MQ=255)
                                                                                                      ggtggCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGCAAAGAGTTGTTCACCCAGGCGGGAATGCTGCGGGTGGGTAAGGCGC‑GGACCGTGCTGCGTGGGGGGAAAGTCCTTAAAGAAGTGGGAGAACAGCAgggggggggaa                                                                                                                                          >  2:94271/1‑231 (MQ=255)
                                                                                                           cATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGGGGATCAGGCGC‑GTACCGGGCTGCGTGAGGGGAAAGGCCTTAAAGAGATGGCAAGCCAGCAGGGCGGGACGTGGTcc                                                                                                                                     >  1:531590/1‑239 (MQ=255)
                                                                                                             ttGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCTGGCTGCGGGGGGGGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGGGGGACGATGTCggg                                                                                                                                   >  2:1011449/1‑239 (MQ=255)
                                                                                                             ttGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGGGTTGTTCACCCAGGCGGGAATGCTGCTGGGGGATCAGGCGC‑GTACCGTGCTGCGGGAGGGGAAAGGCCTTAAAGAGAAGGAAAGCCAGCAGGGCGAGACGATGTCCg                                                                                                                                    >  2:360115/1‑238 (MQ=255)
                                                                                                                   tGAC‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCg                                                                                                                               <  1:164205/238‑1 (MQ=255)
                                                                                                                           cGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGGGGATCAGGCGC‑GTACCGTGCGGCGTGAGGGGAAAGTCCTTAAAGAGATGGCAAGCCACCAGGGGGAGACAATGGCCGGACCGC‑GGcaaaa                                                                                                                      >  2:21395/1‑236 (MQ=255)
                                                                                                                                  ttgggtgTGCGGCAAGTTCCTGCCCGGGTAGCCAGCCGAC‑GCTTAGGGGGCAAATTCGTAAGCTGGAAGATGAGCGGGGCGTGATGTGGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTg                                                                                                               <  2:260027/233‑1 (MQ=255)
                                                                                                                                   ggaaggTGCGGCAGATTCCTGCCAGGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGttt                                                                                                                <  1:812238/232‑1 (MQ=255)
                                                                                                                                        ggccGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTcc                                                                                                          <  1:421255/235‑1 (MQ=255)
                                                                                                                                        gTGGGGAAGATTCCGGCCACGTTAGCCAGCCGAC‑GCTTAGGGGGCAAATTCGTAAGTTGGAAGCTGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGTAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTcc                                                                                                          <  1:754782/238‑1 (MQ=255)
                                                                                                                                              caaatcccggCCACTTTAGCCAGCCGAC‑GGTTAGCGGGAAAATTCTGAAGCTGGAAGATTAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑TTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGt                                                                                                    <  1:125067/229‑1 (MQ=255)
                                                                                                                                                  ttCCTGCCACGTTAGCCGGCCGTC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGa                                                                                                <  1:697129/238‑1 (MQ=255)
                                                                                                                                                  ttCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGa                                                                                                <  1:540918/238‑1 (MQ=255)
                                                                                                                                                   tCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGGGGATCAGGCGC‑GTACCGTGCTGCGTGAGGGGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGAGGTCCGGACCGC‑GCCACATTGGTTTGATTCCCACAgcgggc                                                                                                >  2:812238/1‑232 (MQ=255)
                                                                                                                                                       gCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTAc                                                                                          <  1:435841/239‑1 (MQ=255)
                                                                                                                                                        ccACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGGGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTa                                                                                           >  1:712262/1‑237 (MQ=255)
                                                                                                                                                        ccACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGAGTTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTa                                                                                           >  1:391656/1‑237 (MQ=255)
                                                                                                                                                        ccACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGAAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGGTCAGGCGC‑GTACCGTGCTGCGTGAGGTGGAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCCGACCGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTGggaccggacc                                                                                         >  2:540918/1‑239 (MQ=255)
                                                                                                                                                               agccagccGAC‑GCTTAGGGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTAcc                                                                                  <  1:94271/239‑1 (MQ=255)
                                                                                                                                                                gccagccGGC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCg                                                                                 <  1:561504/239‑1 (MQ=255)
                                                                                                                                                                gccagccGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCg                                                                                 <  1:1020313/239‑1 (MQ=255)
                                                                                                                                                                gcaagccGAC‑GCTTAGCGGGCAAGTTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCg                                                                                 <  1:236322/236‑1 (MQ=255)
                                                                                                                                                                 gccgccGACGGCTTGGCGGGAAAATTGGTAAGCGGAAAGAGGGGCTGGGGGGGATGTGGTGGGGGGGGCCCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCg                                                                                 <  2:1147969/236‑1 (MQ=255)
                                                                                                                                                                  cagccGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCg                                                                                 <  1:872294/237‑1 (MQ=255)
                                                                                                                                                                      cggc‑GCTTAGCGGACAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGGTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCatat                                                                            <  1:913435/235‑1 (MQ=255)
                                                                                                                                                                      cGAC‑GTTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCatat                                                                            <  1:1235307/238‑1 (MQ=255)
                                                                                                                                                                      cGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCatat                                                                            <  1:1011449/238‑1 (MQ=255)
                                                                                                                                                                       gAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGGGATGTTGCTGGAGCGGACAAACCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGGGGATCAGGCGC‑GTACCGTGCTGCGGGGGGGGAAAGACCTTAAAGAGCTGGCAAGCCAGCCAGGCGGAGCGGGGGCCGGGACGCATGC‑CATTGGGTTGATTCCAACAGTGGGGGCGTACCGGCCACCGcccatt                                                                           >  2:348761/1‑233 (MQ=255)
                                                                                                                                                                           gCTTAGGGGGCAAATTCGTAAGCTGGAAGAGGAGCTGGGCGTGATGTTGGTGGAGCGGACCAGGCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATcc                                                                       <  1:314194/239‑1 (MQ=255)
                                                                                                                                                                            tttgcggggCAAGTGCGGAGGCGGGAAGTGGTGCTGGGGTTGTTGTTGCGGGGGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATTCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATcc                                                                       <  2:106157/232‑1 (MQ=255)
                                                                                                                                                                              gacggggCAAATTCGTAAGGTGGAAGGTGGGCTGGGCGGGATGTTGCGGGGGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATccc                                                                      <  2:759470/233‑1 (MQ=255)
                                                                                                                                                                                  gggCAAAGTCGGAAGCTGGAAGTTGAGCTGGGCGTGATGTTGCTGGGGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGAACGTACCTGCTACCGCATATTATCCCTAtgc                                                                 <  1:383312/238‑1 (MQ=255)
                                                                                                                                                                                       aaTTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCg                                                                                 <  1:95737/217‑1 (MQ=255)
                                                                                                                                                                                           cggaaGGTGGAAGATGAGCGGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGAc                                                       <  1:953246/236‑1 (MQ=255)
                                                                                                                                                                                                    gAAGATGGGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCaa                                               <  1:798855/238‑1 (MQ=255)
                                                                                                                                                                                                      agttggggtGGGCGTGTTTTTGCGGGGGGGGCCCCGCCGTAAAGGGTTGTTCCCCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAg                                             <  2:712262/230‑1 (MQ=255)
                                                                                                                                                                                                      aGATGAGCTGGGCGTGATGTTGCTGGGACGGCCCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGaa                                                                                                                                                                                <  2:188711/108‑1 (MQ=255)
                                                                                                                                                                                                      aGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTcc                                                                                                          <  1:1017998/177‑1 (MQ=255)
                                                                                                                                                                                                           ggttGGGCGTTTTGTTTGTGGGGCGGCCCCGCCGTAATGTGTTGTTCACCGAGGAGGAAATGCGGATTGTGGATCCGGCGC‑GTACCGTGCTGCATGAGGTGAAAGTCCTGAAAGAGATGGCAAGCCAGCAGGGCGAGACCATGTCCGGACCGG‑TGCACACTGGTTTGATTCCCACAGTTGGGCCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGaa                                       <  2:647487/236‑1 (MQ=255)
                                                                                                                                                                                                           ggtgtgggttgtgtttccgggggCGGCCCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGaa                                       <  2:391656/217‑1 (MQ=255)
                                                                                                                                                                                                           aGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGGGAGGTGAAAGTCCTTAAAGAGATGGGAAGCCAGCAGGGCGAGACGAGGTCCGGACCGC‑TGCACATTGGGTTGCTTCCCACAGTTGGACCCTACCGGCTCCCGCATATTATCCCTATACTGCACCAGACCTTTCCAAAGCTGGaa                                       >  2:787804/1‑239 (MQ=255)
                                                                                                                                                                                                                ggCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATTCTGCTGGTGGATCAGACGC‑TTACCGTGCTGCGTGAGGTGAAAGTCCTTaaa                                                                                                                                                                      >  1:188711/1‑108 (MQ=255)
                                                                                                                                                                                                                ggCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGAc                                                                                                                                 <  1:610711/145‑1 (MQ=255)
                                                                                                                                                                                                                ggCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGAAGATGTCCGGAc                                                                                                                                 >  2:610711/1‑145 (MQ=255)
                                                                                                                                                                                                                  ggggtttttggggggggggACCAGCGGGAAAGTTTTGTTCCCCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTAt                                 <  2:104990/221‑1 (MQ=255)
                                                                                                                                                                                                                  cGTGTTGTTGCTGGGCGGGACCGGCCGTAAAGGGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCATAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTAt                                 <  2:545138/238‑1 (MQ=255)
                                                                                                                                                                                                                        ggtGCTGGAGGGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATg                          <  1:1365676/237‑1 (MQ=255)
                                                                                                                                                                                                                        gTTGCGGGAGGGGACCAGCCGTAAAGTGTTGTTCGCCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGGTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATg                          <  1:1355406/239‑1 (MQ=255)
                                                                                                                                                                                                                         gtGCTGGAGGGGACCAGCCGTAAAGGGTGGTTCACCCGGGCGGGAATGCTGCGGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATg                          <  1:787804/237‑1 (MQ=255)
                                                                                                                                                                                                                            gtgggggggACCAGCCGTAAAGTGTTGTTCCCCCAGGCGGGAATGCTGCTGGTGGATCAGGCGG‑GTACCGTGCTGCGTGAGGTGAAAGTCCCTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGc                      <  2:189977/232‑1 (MQ=255)
                                                                                                                                                                                                                              ggAGCGGACCAGGCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGTAACAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCAAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGcac                    <  1:165490/239‑1 (MQ=255)
                                                                                                                                                                                                                                  cGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGGGAAAGTCCTTAAAGGGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGCCCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGGACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAACACAGa                 >  2:1155006/1‑238 (MQ=255)
                                                                                                                                                                                                                                               aaGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGAATCTGCATGAAGCACAGACCCACCAGTTAcgg   >  1:936775/1‑237 (MQ=255)
                                                                                                                                                                                                                                                aGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTgg  <  1:1014891/239‑1 (MQ=255)
                                                                                                                                                                                                                                                  tgttgtTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTgg  <  1:601759/237‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              
AATGCTTGATAGGGATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTT‑GAGTACCTGGTGGCATTGGC‑TGAA‑CACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGAC‑GCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGC‑GTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGC‑TGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGG  >  NC_000913/4158414‑4158888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A