Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I0 R1 54 59.4 1320120 91.9% 1213190 218.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,158,652 T→G 15.6% V55G (GTG→GGG)  oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,158,6520TG15.6% 170.5 / 2.1 93V55G (GTG→GGG) oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (29/46);  new base G (14/3);  total (44/49)
Fisher's exact test for biased strand distribution p-value = 2.28e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

ATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGAC  >  NC_000913/4158428‑4158874
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                               
aTAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGCCCCGCCGTAAA‑Gggtt                                                                                                                                                                                                                              >  2:281099/1‑228 (MQ=255)
aTAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑gtgct                                                                                                                                                                                                                              >  2:315384/1‑226 (MQ=255)
   aTCGTTCATTGCTATTCTACCTATCGCAATGAACTATCGTGGCGATGGAGGATGGATACTGAATATT‑CGTGTTCTTGAGTACCTGGTGGCATTGGCTGCACACCGCCATTTTCGGCGTGCGGCAGAGTCGTGCCCCGTTAGCCAGCCGACGCTTAGCGGGC‑AATTAGTAAACATGTAAGCTGAGCTGGGCGTGATGTTGGGGGAGCGGCACAGGCGGAAA‑GTgtggtt                                                                                                                                                                                                                           >  1:481022/1‑228 (MQ=255)
        ttaTTGCTATTCTAGCTATCGCCGTGAGCTATCGTGGCGATGGTGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAAAACCTCCATTTTCGGCGTGCGGCGGATTCCTGCAACGTTAGCCAGCCGACGCTTAGCGGGTAAATTCGTAAGC‑AGGAAGTAGAGCTGCGCGAAAGGTTGGGGGAGCGGACCAGCCGTCAA‑TGGTGGTTccac                                                                                                                                                                                                                       >  1:481389/3‑224 (MQ=255)
        tCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTGGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCAccc                                                                                                                                                                                                                      >  2:230743/1‑228 (MQ=255)
        tCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATATTCGGCGTCCGGCAGATTCCTGCCACGTTAGCCAGCCGCCGCTTATCGGGGACATTCGTAAGC‑TGGAAGATGAGCTGGGCGGGATGTGGCGGGAGCGGAACCGCGCTAAATGTTTTGTTcccc                                                                                                                                                                                                                       >  1:510971/1‑228 (MQ=255)
         cATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCGTATCGGGCAAATTCGTAAGC‑TGGCAGATGAGCTGGGCGTGCTGTTGCTGGAGCGGACCACCCGTAAA‑GGGTTGTTACCCCa                                                                                                                                                                                                                     >  1:205467/1‑228 (MQ=255)
                 ttCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGCCCCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTAACCCAGGCGGGaa                                                                                                                                                                                                             >  2:495669/1‑228 (MQ=255)
                      ccTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGCTGAGCGTGGCGTGATGTTGCTGGGGCGGACCACCCGTAAA‑GGGTAGTTCACCCCGGGGGGATtggtg                                                                                                                                                                                                        >  1:559386/1‑228 (MQ=255)
                      ccTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GGGTTGTTAACCCAGGGGGGAAtgctg                                                                                                                                                                                                        >  2:521038/1‑228 (MQ=255)
                      ccTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAACC‑TGGAAGATTAGCTGGGCGTGATGTTGCTGGAACGGCCCAGCCGGGAA‑GGGTTGTT‑ACCCCCGCGGGaaaagatg                                                                                                                                                                                                       >  1:457974/1‑222 (MQ=255)
                        tATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGGGATGTTGCTGGAGCGGACCAACCGTAAA‑GTGTTGTTCACCCAGGCGGGAATgctgct                                                                                                                                                                                                      >  2:97644/1‑228 (MQ=255)
                         aTCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGGGATGGGCGGGATGTTGCTGGAGCGTACCAGCCGTAAA‑GTGTTGTTCACCCAGGGGGGAAAGcggctg                                                                                                                                                                                                     >  1:160495/1‑228 (MQ=255)
                         aTCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAGTGttgctg                                                                                                                                                                                                     >  2:588696/1‑228 (MQ=255)
                         aTCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACCCTTAGCGGGCCAATTCGTAAGC‑TGGAAGATGAGCTGGGCGGGCTGTTGCTGTGTCGGGCCAGCCCTAAA‑GTGGTTTCCACACAGGCGGGAATGctgctg                                                                                                                                                                                                     >  1:562265/1‑228 (MQ=255)
                           cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGGGCTGGGCGGGATGTTGCTGGAGCGGACCAGCCGTAAA‑GGGTTGTTCAACCAGGCGGGAAAGGtggtggg                                                                                                                                                                                                   >  1:439939/1‑227 (MQ=255)
                           cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTAACCCAGGCGGGACTGCTGCtggt                                                                                                                                                                                                   >  2:274445/1‑228 (MQ=255)
                           cGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCGGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGCTGAGCTGGGCGGGATGTTGCTGGAGCGGACCGGCCGTAAA‑GCGTGTTTCACCAGGGCGGGGATGTTGCtggg                                                                                                                                                                                                   >  1:407064/1‑227 (MQ=255)
                              cATGAACTATCGTGGCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTACA‑GGGTTGTTCACCCAGGCGGGAATGCtggtggtgg                                                                                                                                                                                                 >  1:573263/1‑227 (MQ=255)
                                 gaccttgcGTGGGGATGGAGGATGGATAATGAAATTT‑CGTGATCTGGAGTACCGGGTGGCATTGGCTGACCACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGAt                                                                                                                                                                                               <  2:371592/219‑1 (MQ=255)
                                    ctttCGGGGCGATGGAGGATGATTAATGAATATT‑CGTGCTTTTGGTTCCTTGGTGGCATTGGCTGACCACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGgc                                                                                                                                                                                          <  2:439939/225‑1 (MQ=255)
                                       tCGTGGCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGGGATGTTGCTGGAGCGGGCCACCCGTAAA‑GGGGTGTTCACCCAGGCGGGAAGTGTGCTGGGGGACAAGgcggg                                                                                                                                                                                       >  1:371592/1‑226 (MQ=255)
                                         gggggaatgggcgatggttAATG‑ATATTGCGTGGCTTTGAGTCCTGGGTGGCTTTGGCTTAACACCGCCATTTTCGGCGTTCGGCAGATTCCTGCCACATTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTa                                                                                                                                                                                     <  2:160555/216‑1 (MQ=255)
                                            gCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTACTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGGGTGATGTTGCTGGAGCGGACCAGCCGCAAA‑GGGGAGTTACCCCAGGCGGGAATCTTGGTGTTGGGTCAGTCGGGTACCg                                                                                                                                                                                  >  2:256206/1‑228 (MQ=255)
                                               aTGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGTTAAGCTGGCCGTGATGTTGCTGGAGCGGACCACCCGTAAA‑GTGTTTTTCACCAAGGCGGGAATGCTCCTGGGGGGTCAGGCGCTAACCggtc                                                                                                                                                                               >  2:121824/1‑225 (MQ=255)
                                                tGGAGGATGGATAAGGAATATT‑CGTGATCTTGAGTACCGGGTGGCATGGGCTGAACCCCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGtgct                                                                                                                                                                              <  2:443496/228‑1 (MQ=255)
                                                 ggaggaTGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGGCTACCctgctg                                                                                                                                                                             >  2:390439/1‑228 (MQ=255)
                                                       gggatAATGAATGTT‑GTGGACCTTGAGTACCTGGTGGCATTGGCTAACCACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGa                                                                                                                                                                       <  2:513950/226‑1 (MQ=255)
                                                          atAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGGGGAGCAGgccgggaccgggcgcggtgagggg                                                                                                                                                                    >  1:116499/1‑206 (MQ=255)
                                                          atAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGGAAA‑GGGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGGGCTT‑CGGGAgggg                                                                                                                                                                   >  2:319361/1‑226 (MQ=255)
                                                            agtGAATATG‑CGGGATCGTGAGTACCGGGGGGCATTGGCTAAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGGGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGaa                                                                                                                                                                 <  2:59090/226‑1 (MQ=255)
                                                             aTGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGGATGCGGCTGGTGGATCAGGCGGGTCCCGGGCTG‑Cgggggggggaa                                                                                                                                                                >  1:436357/1‑218 (MQ=255)
                                                              taaaaggaacgggATGTTGGGTCCCGGGGGGCAGGGGTTCAACCCCGCCATTTTCGGCGGGGGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGaa                                                                                                                                                                 <  1:434568/215‑1 (MQ=255)
                                                              agAATTTT‑CGTTATCTTGAGTACTGGGGGGAATGGGCTGAACCCCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGaa                                                                                                                                                                 <  2:436357/225‑1 (MQ=255)
                                                               tgaatttgggTGATGTTGAGTACCTGGTGCCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGaaa                                                                                                                                                                <  2:391970/218‑1 (MQ=255)
                                                               gAATATT‑CGTGATCTTGAGTCCCTGGTGGCAGTGGATGACTACCGTCATTTTCGGCGTGCGGCAGATTCCTGCCGAGTTAGCCAGCCGACGCGTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAg                                                                                                                                                               <  2:347282/227‑1 (MQ=255)
                                                                aaTATT‑CGTGATTTTGAGTACCTGGTGTCATGGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGGTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAg                                                                                                                                                               <  2:611712/226‑1 (MQ=255)
                                                                    tt‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATTTTGCTGGAGCGGACCAGCCGTAAA‑GGGTGGTTCACCCAGGGGGGAATGCTCCTGGTGGATCAGGCGCGTACCGGGCGG‑CGTGGGGGGAAAGACCTTa                                                                                                                                                         >  1:611712/1‑228 (MQ=255)
                                                                           aTCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGCTGTTCCTGGGGCGGACCAGCCGTAAA‑GGGTTGTTCACCCAGGCGGGAATGCTGCCGGTGAGTCAGGCGCGGACCCTGTCG‑GGGGAGGGGAAAGGCCTTAAAgaga                                                                                                                                                   >  1:310709/1‑228 (MQ=255)
                                                                           aTCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGAGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAAGGCTGGTGGGGGAGCAGTGGGGGACCGCGGTG‑CGGGGGGGGaaaagccatcaaaaaa                                                                                                                                                   >  1:516872/1‑215 (MQ=255)
                                                                                     ccTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGGGCTG‑CGTGGGGGGAAAGGCCTTAAagccgtctctttta                                                                                                                                          >  2:434568/1‑215 (MQ=255)
                                                                                     ccTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGGGATGTTCCTGGAGCGGACCAGCCGGAAA‑GTGTTGTTCACCCAGGCGGGAACGGTGCTGGGGGTGCAGGCGCGTACCGTGCTG‑CGGGGGGGGAAGAGCCTTAAagagcagggaaaccc                                                                                                                                         >  1:525658/1‑217 (MQ=255)
                                                                                      cTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGGATGCTGCTGGTGGACCAGGCGCGTACCGTGCCG‑CGTGGGGTGACAGTCCTTCAAGAGATGGCAAGcccg                                                                                                                                        >  2:484983/1‑226 (MQ=255)
                                                                                      cTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGGGGGTCAGGGGCGGACCGTGCTGCCGTG‑GGGGAAAGTCCTTAAAGGGAGGGAAAGCcag                                                                                                                                        >  1:245131/1‑228 (MQ=255)
                                                                                        ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGGGAAAGGCCTTAAAGAGATGGCAAGCcagca                                                                                                                                      >  2:336317/1‑228 (MQ=255)
                                                                                        ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTAACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGCGGTGAAAGGCCTTAAAGAGAGGGCAAGccacca                                                                                                                                      >  2:200054/1‑228 (MQ=255)
                                                                                        ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGCCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GGGTAGTTCACCCAGGCGGGAATGCTGCTGGGGGATCAGGCGCGTACCATGCTG‑CGAGAGGTGGAAGTCCTTAAAACGATGGAAAGCccgca                                                                                                                                      >  1:322926/1‑228 (MQ=255)
                                                                                           ggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGGTGCCGGGGGGTCAGGGCCGTACCGTGCGG‑GCGGAGGGGAAAGCCCTTAAAAAAAGGGCAAGCCAGCAggg                                                                                                                                   >  1:250903/1‑228 (MQ=255)
                                                                                                 ggcgaacccccGCCTTTTTGGGGGGGCGGCAGTTTCCTGCCAGGTTAGGCAGCCGGCGCTTGGGGGGCAAATTCGGAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGAc                                                                                                                             <  1:336317/219‑1 (MQ=255)
                                                                                                  gtgaaaacccGCCATTGTGGGGGTCGGGCAGATTCCTGCAACGTTGGCCGGCCGACGCTTAGGGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGAGGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGCGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACg                                                                                                                            <  1:258177/220‑1 (MQ=255)
                                                                                                           cccctttttGGGGGTGCGGCAGATCCCGGCCAGGTTAGCCGGGCGACGTTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGGGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGa                                                                                                                   <  1:95075/223‑1 (MQ=255)
                                                                                                           cGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGGTGTCCGGa                                                                                                                   >  2:534144/1‑228 (MQ=255)
                                                                                                                     ggCGTGCGGTAGCTTCCTGCTAGGTTTGCCGGCCGACGCTTAGGGGGCAAATTAGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGcaca                                                                                                         <  2:205467/228‑1 (MQ=255)
                                                                                                                          gCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCCTAAA‑GGGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCATGCGCGTACCTAGCTT‑CGTGGGGGAAAAGGCCTTCAAGAGATGGCAAGCCAGCAGGGCGGAACGAGGTCCCGACCGCTGCACCTTGGt                                                                                                    >  1:175398/1‑228 (MQ=255)
                                                                                                                           cGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGGGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCAACTTGGtt                                                                                                   >  2:12015/1‑228 (MQ=255)
                                                                                                                                   tCCTGGCAGGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTcc                                                                                            <  2:457974/227‑1 (MQ=255)
                                                                                                                                     ctgcagggTTAGCCAGGCGACGCTTGGCGGGCAAATTCGGAAGC‑TGGAAGGTGGGCTGGGCGTGATGTTGCTGGAGGGGGCCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCca                                                                                          <  2:322926/220‑1 (MQ=255)
                                                                                                                                       gccgtgttcggcaggcGGCGGTTAGCGGGCAAGTGCGTAAGC‑TGGAAGATGGGCTGGGCGGGGTGGTGGTGGTGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAg                                                                                       <  1:495669/217‑1 (MQ=255)
                                                                                                                                           cGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTg                                                                                    <  2:245131/227‑1 (MQ=255)
                                                                                                                                            tttAGCCAGCCGGAGTTTGGGGGGAAAATTCGGAAGC‑GGGAAGTAGGGCTGGGCGGGAGTGGGAGGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGa                                                                                  <  1:274445/227‑1 (MQ=255)
                                                                                                                                             ttgcccggcgggcggttggCGGGAAAATTCGTAAGC‑TGGAAGGTGGGGGGGGCGGAGGGGTGCGGGCGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGa                                                                                  <  1:376227/209‑1 (MQ=255)
                                                                                                                                              tggccagccGAGGTTTGGCGGGAAAATTCGGAAGC‑TGGAAGATGGGCTGGGGGGGTTGTTGCTGGGGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGAcc                                                                                <  1:534144/226‑1 (MQ=255)
                                                                                                                                                    gccGACGGTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGGGCGGACCAGCCGTAAA‑GGGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTAcc                                                                           <  2:328450/227‑1 (MQ=255)
                                                                                                                                                     cggtcgtttgggggggAAATTGGTAAGG‑TGGAAGGTGGGCTGGGGGGGATGGTGCGGGGGGGGGCCAGGCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTg                                                                         <  1:315384/216‑1 (MQ=255)
                                                                                                                                                      cGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCAACCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGGACAGTGCTG‑CGTGAGGTGAAAGTCCTTACAGAGATGGCAAGCACGCAGGGCGAGACGATGTCCGAGACGCTGCCCATTGGATTGATTCCcacacgggaagcggaccctc                                                                        >  1:247757/1‑212 (MQ=255)
                                                                                                                                                           gttAGCGGGCAAATTGGTAAGC‑TGGAAGATGAGCGGGGGGGGAGGTTGCGGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCg                                                                   <  2:460736/227‑1 (MQ=255)
                                                                                                                                                               gCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCat                                                                >  2:8052/1‑227 (MQ=255)
                                                                                                                                                                ggggAAAATTCGTAGGC‑TGGAAGATGAGGGGGGTGTTGTTGTGCTGGGGGGGCCCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCatat                                                              <  1:588696/227‑1 (MQ=255)
                                                                                                                                                                 gggCAAATTCGTAAGG‑TGGAAGATTAGCTGGGCGTGATGTTGCTGGATCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCatat                                                              <  2:160495/227‑1 (MQ=255)
                                                                                                                                                                 gggCAAATTCGTAAGC‑TGGAAGATAAGCGGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCatat                                                              <  2:472698/227‑1 (MQ=255)
                                                                                                                                                                     aattttgTAAGG‑TGGAAGGTTGGGTGGGGGTGATTTTGGGGGGGGGGACCAGCCGTAAA‑GGGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGGGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATcc                                                         <  1:97644/222‑1 (MQ=255)
                                                                                                                                                                     aaaTTGGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATcc                                                         <  2:573263/228‑1 (MQ=255)
                                                                                                                                                                     aaaTTCGTAAGC‑TGAAAGATGGGCGGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCCCCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATcc                                                         <  2:250903/228‑1 (MQ=255)
                                                                                                                                                                        tgcggaaGC‑TGGAAGGTGGGCGTGGCGGGATGTTGCTGGAGCGGACCTGCCGTAAA‑GTGTTGTTCGCCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTa                                                      <  2:407996/223‑1 (MQ=255)
                                                                                                                                                                          tgTAAGG‑TGGAAGGTGGGGTGGGGGTTGTGTTGCGGGGGGGGACCGGCCGTAAA‑GTGTTGTTCCCCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtg                                                    <  1:12015/227‑1 (MQ=255)
                                                                                                                                                                            tAAGC‑TGGAAGAGGAGCTGGGCGTGATGGTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtg                                                    <  2:407064/226‑1 (MQ=255)
                                                                                                                                                                            tAAGC‑TGGAAGACGAGCTGGGCGTGATGTTGGTGGAGGGGACGAGCCGTAAA‑GGGTTGTGCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgc                                                   <  2:510971/227‑1 (MQ=255)
                                                                                                                                                                             gaaagtagaaatgggaggggggGTGATTGTGGTGGGGGGGCCCAGCCGTAAA‑GTTTTGTGCCCCCGGGCGGGAATTCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgc                                                   <  1:495023/209‑1 (MQ=255)
                                                                                                                                                                                    gAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGGGACGATGTCCGGACCGCTGCCCATTGGGTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAg                                           >  2:271417/1‑228 (MQ=255)
                                                                                                                                                                                    gAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCt                                                                          >  2:623500/1‑197 (MQ=255)
                                                                                                                                                                                            gCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTcc                                   >  2:463532/1‑228 (MQ=255)
                                                                                                                                                                                              tggggggagttttgggggAGGGGGCCAGCCGGAAA‑GTGTTGTTCCCCCGGGCGGGAATGCTGCTGGTGGATCAGGCGCGGACCGGGCTG‑CGTGAGGTGAAAGTCCTGAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCaa                                 <  1:463532/212‑1 (MQ=255)
                                                                                                                                                                                                    tggtgtgGCTGGCGCGGACCAGCCGTAAA‑GTGTTGTTCCCCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCt                             <  2:525658/223‑1 (MQ=255)
                                                                                                                                                                                                       tGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACg                                                                                                                            <  1:306891/128‑1 (MQ=255)
                                                                                                                                                                                                        gttgtggggggggcccAGCCGTAAA‑GTGTTGTTCCCCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGaaa                        <  1:319361/213‑1 (MQ=255)
                                                                                                                                                                                                             tGGGGCGGCCCAGCCGTAAA‑GTTTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTAt                   <  2:562265/227‑1 (MQ=255)
                                                                                                                                                                                                             ggggagggACCCGCCGGTAA‑GTGTTGGTCGACCAGGCGGGAATGCGGCTGGTGGAACAGGCGCGATCCGGGCTG‑CGTGGGGTGAAAGTGCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCATACCTTTCCAAAGCTGGAAATGTAt                   <  2:310709/221‑1 (MQ=255)
                                                                                                                                                                                                               gggcggccgcgacGTAAA‑GTGTTGTTGCCAGAGGCGGGAGTGGCGCTGGTGGGACAGGCGCGGGCGGTGCTG‑CGGGAGGTGAAAGGCCTTAAATAGATGGCAAGCCAGCAGGGGGGGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCt                 <  1:484983/215‑1 (MQ=255)
                                                                                                                                                                                                                  cGGGCCAGCCGTAAA‑GTGTTTTTCACCCGGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTTCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCa              <  1:307769/227‑1 (MQ=255)
                                                                                                                                                                                                                   ggACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGGCGATGTCCGGACCGGTGCACATTGGTTTGATTCCCACAGTGGGAACGTACCTGCTCTCGCATATTATCCCTATTCTGAACCAGACCTTTCCCAAGCTGGAAATGAACCTGCAt             >  2:168679/1‑227 (MQ=255)
                                                                                                                                                                                                                        aGCCGTAAA‑GTGTGTTTCACCCAGGCGGGAATGCTGCTGGTTGTTCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGGCCTTAAAGAGATGGCAAGCCAGCATGGCGATACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGTCCTTTCCAAAGCTGGAAATGTATCTGCATGAAGca       <  2:481022/228‑1 (MQ=255)
                                                                                                                                                                                                                         gcgtaaaa‑GTGTTGGTCCCCCAGGCGGGAATTCTGCTGGTGGATGAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGcac      <  1:230743/223‑1 (MQ=255)
                                                                                                                                                                                                                          ccGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGcac      <  2:559386/227‑1 (MQ=255)
                                                                                                                                                                                                                              aaa‑GTGTTGTTCACCCAGGGGGGAATGCTGCTGGTGGATCAGGGCGGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGAc  <  2:490018/227‑1 (MQ=255)
                                                                                                                                                                                                                              aaa‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGAc  <  2:116499/227‑1 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                               
ATAATCGTTCATTGCTATTCTACCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATT‑CGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGC‑TGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAA‑GTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTG‑CGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGAC  >  NC_000913/4158428‑4158874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A