Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F1 I1 R1 770 51.4 2439994 93.8% 2288714 110.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,897,050 +G coding (277/666 nt) yieH → phosphoenolpyruvate and 6‑phosphogluconate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,897,0461.G100.0% 203.8 / NA 58E91E (GAA→GAG) yieHphosphoenolpyruvate and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (30/28);  total (30/28)

TTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑GGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCT  >  NC_000913/3896908‑3897179
                                                                                                                                           |                                                                                                                                     
tttCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                        <  2:1110359/139‑1 (MQ=255)
tttCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                        <  1:237317/139‑1 (MQ=255)
 ttCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                        <  1:102140/138‑1 (MQ=255)
 ttCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                        >  2:102140/1‑138 (MQ=255)
      aGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑gggggc                                                                                                                                 <  2:460633/139‑3 (MQ=255)
         tgtAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑gggggctgg                                                                                                                              >  1:9196/1‑134 (MQ=255)
           tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAg                                                                                                                             >  2:196631/1‑139 (MQ=255)
           tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAg                                                                                                                             >  1:726755/1‑139 (MQ=255)
           tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAg                                                                                                                             >  1:107840/1‑139 (MQ=255)
           tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAg                                                                                                                             >  2:772884/1‑139 (MQ=255)
           tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAg                                                                                                                             >  1:442587/1‑139 (MQ=255)
            aaaaCTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAgc                                                                                                                            <  1:386812/139‑1 (MQ=255)
                  gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCt                                                                                                                      >  2:558999/1‑139 (MQ=255)
                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                        <  1:258784/120‑1 (MQ=255)
                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                        >  2:258784/1‑120 (MQ=255)
                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTg                                                                                                                     >  1:662945/1‑139 (MQ=255)
                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTg                                                                                                                     <  2:269966/139‑1 (MQ=255)
                           tATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGAt                                                                                                             >  2:325532/1‑139 (MQ=255)
                                    tGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCTTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGcc                                                                                                    >  1:919460/1‑139 (MQ=255)
                                    tGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGcc                                                                                                    <  2:107840/139‑1 (MQ=255)
                                     gTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa                                                                                                   <  2:9196/139‑1 (MQ=255)
                                            ttGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                            <  2:662945/139‑1 (MQ=255)
                                              gAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGTTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtgtg                                                                                          <  2:734396/139‑1 (MQ=255)
                                                     gtgtTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTa                                                                                   <  1:772884/139‑1 (MQ=255)
                                                        ttACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                        >  1:528447/1‑83 (MQ=255)
                                                        ttACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                        <  2:528447/83‑1 (MQ=255)
                                                        ttACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCAACGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACg                                                                                <  2:167213/139‑1 (MQ=255)
                                                         tACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACgg                                                                               >  1:413463/1‑139 (MQ=255)
                                                          aCGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                            >  1:715068/1‑125 (MQ=255)
                                                          aCGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                            <  2:715068/125‑1 (MQ=255)
                                                              tAGCGAAAACAGACGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCa                                                                           <  2:549039/138‑1 (MQ=255)
                                                              tAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCa                                                                           >  1:549039/1‑138 (MQ=255)
                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                            <  2:207114/119‑1 (MQ=255)
                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                            >  1:207114/1‑119 (MQ=255)
                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCaaac                                                                        >  2:1126047/1‑138 (MQ=255)
                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAAt                                                                        >  1:1043790/1‑139 (MQ=255)
                                                                            gCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcag                                                            <  1:325532/139‑1 (MQ=255)
                                                                                aaCACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCAtt                                                        >  1:853144/1‑139 (MQ=255)
                                                                                 acacGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTc                                                       <  2:726755/139‑1 (MQ=255)
                                                                                     gTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATg                                                   >  1:462607/1‑139 (MQ=255)
                                                                                        tACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc                                                <  2:586674/139‑1 (MQ=255)
                                                                                           cGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                        <  2:680679/48‑1 (MQ=255)
                                                                                           cGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                        >  1:680679/1‑48 (MQ=255)
                                                                                             tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTa                                                                                   >  2:863161/1‑99 (MQ=255)
                                                                                             tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTa                                                                                   <  1:863161/99‑1 (MQ=255)
                                                                                             tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATg                                                               >  2:348722/1‑119 (MQ=255)
                                                                                             tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATg                                                               <  1:348722/119‑1 (MQ=255)
                                                                                             tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCt                                           >  1:768648/1‑139 (MQ=255)
                                                                                             tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCt                                           >  1:288826/1‑139 (MQ=255)
                                                                                               cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                        >  1:1188700/1‑44 (MQ=255)
                                                                                               cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                        <  2:1188700/44‑1 (MQ=255)
                                                                                               cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACt                                                                                                         >  1:812457/1‑75 (MQ=255)
                                                                                               cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGa                                         >  1:341335/1‑139 (MQ=255)
                                                                                               cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGAGCTCCTGTCAGCGATCACt                                                                                                         <  2:812457/75‑1 (MQ=255)
                                                                                                    gTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATg                                    <  2:442587/139‑1 (MQ=255)
                                                                                                       gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGcc                                                                                                    >  1:1080318/1‑72 (MQ=255)
                                                                                                       gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGcc                                                                                                    <  2:1080318/72‑1 (MQ=255)
                                                                                                       gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa                                                                                                   <  2:234598/73‑1 (MQ=255)
                                                                                                       gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa                                                                                                   >  1:234598/1‑73 (MQ=255)
                                                                                                         tCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCa                               <  2:288826/139‑1 (MQ=255)
                                                                                                            gCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATggg                                                 <  2:200274/118‑1 (MQ=255)
                                                                                                            gCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATggg                                                 >  1:200274/1‑118 (MQ=255)
                                                                                                                     ttCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc                                                >  2:385817/1‑110 (MQ=255)
                                                                                                                     ttCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc                                                <  1:385817/110‑1 (MQ=255)
                                                                                                                          aaCTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACaaa                                                                  >  1:1089908/1‑87 (MQ=255)
                                                                                                                          aaCTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACaaa                                                                  <  2:1089908/87‑1 (MQ=255)
                                                                                                                               gAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTc         <  2:768648/139‑1 (MQ=255)
                                                                                                                                aaGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCa        <  1:558999/139‑1 (MQ=255)
                                                                                                                                  gCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCaa                                                                          <  1:145738/71‑1 (MQ=255)
                                                                                                                                  gCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCaa                                                                          >  2:145738/1‑71 (MQ=255)
                                                                                                                                    cATCGAAGGGGGCCGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGTACTACTTCCCGGATAAACTGTTCAGCgg    >  1:596015/1‑139 (MQ=255)
                                                                                                                                      tCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCt  <  2:93180/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                     
TTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑GGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGGCT  >  NC_000913/3896908‑3897179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A