Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,897,050	+G	coding (277/666 nt)	yieH →	phosphoenolpyruvate and 6‑phosphogluconate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,897,046	1	.	G	98.5%	229.6 / ‑3.7	66	coding (273/666 nt)	yieH	phosphoenolpyruvate and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (32/33); total (33/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

TTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑GGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGG  >  NC_000913/3896908‑3897177

tttCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                       <  2:823469/139‑1 (MQ=255)

tttCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                       <  1:69891/139‑1 (MQ=255)

tttCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                       >  2:767323/1‑139 (MQ=255)

     aaGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑ggg‑gg                                                                                                                                <  2:816676/139‑2 (MQ=255)

         tgtAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑ggg‑ggctgg                                                                                                                            >  2:190439/1‑134 (MQ=255)

          gtAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGa                                                                                                                            <  1:426260/139‑1 (MQ=255)

          gtAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑ggg‑ggcagga                                                                                                                           <  1:876039/139‑7 (MQ=255)

                  gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCt                                                                                                                    >  2:1478710/1‑139 (MQ=255)

                  gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑GGGTGCTGGAGCGCTCCt                                                                                                                    >  2:774104/1‑139 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                       >  2:1634229/1‑120 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                       <  1:1634229/120‑1 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAgc                                                                                                                          >  2:110925/1‑132 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAgc                                                                                                                          <  1:110925/132‑1 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTg                                                                                                                   >  1:817677/1‑139 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACG‑TAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGt                                                                                                                  >  2:241918/1‑139 (MQ=255)

                    aCGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGt                                                                                                                  <  2:817677/139‑1 (MQ=255)

                             tCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCa                                                                                                         >  1:1349573/1‑139 (MQ=255)

                                   ttGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑g                                                                                                                                     >  2:3713/1‑105 (MQ=255)

                                   ttGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑g                                                                                                                                     <  1:3713/105‑1 (MQ=255)

                                    tGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGcc                                                                                                  >  1:102270/1‑139 (MQ=255)

                                      tttCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa                                                                                                 >  1:715270/1‑138 (MQ=255)

                                      tttCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa                                                                                                 <  2:715270/138‑1 (MQ=255)

                                        tCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtg                                                                                              >  1:972800/1‑139 (MQ=255)

                                        tCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtg                                                                                              <  1:160354/139‑1 (MQ=255)

                                           cTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgt                                                                                           >  1:848930/1‑139 (MQ=255)

                                             tGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtgt                                                                                         >  2:1572191/1‑139 (MQ=255)

                                              gAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtgtg                                                                                        >  2:220322/1‑139 (MQ=255)

                                               aaCATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTgg                                                                                       >  1:1238044/1‑139 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCt                                                                                  >  2:234119/1‑139 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCt                                                                                  >  2:1223584/1‑139 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCt                                                                                  >  2:854031/1‑139 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTCCGCCAAtgtgtgtccgatct                                                                                  >  2:412049/1‑132 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑ggg‑ggctg                                                                                                                             >  2:209638/1‑91 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑ggg‑ggctg                                                                                                                             <  1:209638/95‑5 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTAACGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCGCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCt                                                                                  >  1:464333/1‑139 (MQ=255)

                                                      tgtTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTaa                                                                                <  2:972800/139‑1 (MQ=255)

                                                          aCGTTAGCGAAAACAGAAGCTGAACACGTTTACCGCGCAGAAGTCGCTCGGCTGTTCGATTGAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGc                                                                            <  2:1199517/139‑1 (MQ=255)

                                                              tAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGccc                                                                          <  2:849222/137‑1 (MQ=255)

                                                              tAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGccc                                                                          >  1:849222/1‑137 (MQ=255)

                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa                                                                                                 >  2:87401/1‑113 (MQ=255)

                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa                                                                                                 <  1:87401/113‑1 (MQ=255)

                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCaaa                                                                       <  1:767323/139‑1 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCg                                                                                                             <  2:863929/100‑1 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCg                                                                                                             >  1:1387982/1‑100 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCg                                                                                                             <  2:1387982/100‑1 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCg                                                                                                             >  1:863929/1‑100 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    >  1:4016/1‑109 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    <  2:4016/109‑1 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAAt                                                                      >  2:723283/1‑139 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAAt                                                                      >  2:1097043/1‑139 (MQ=255)

                                                                     aaCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                          <  2:1287615/114‑1 (MQ=255)

                                                                     aaCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                          >  1:1287615/1‑114 (MQ=255)

                                                                      aCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACaaa                                                                <  1:220322/139‑1 (MQ=255)

                                                                         gAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATg                                                             <  2:1322003/139‑1 (MQ=255)

                                                                         gAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATg                                                             <  1:241918/139‑1 (MQ=255)

                                                                             cTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcagc                                                         <  2:1356764/139‑1 (MQ=255)

                                                                             cTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcagc                                                         <  1:1572191/139‑1 (MQ=255)

                                                                              tGAACACGTTTACCGGGCAGAAGTCGCTCGGCTGTTTGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcagca                                                        <  2:202628/139‑1 (MQ=255)

                                                                                   acGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTa                                                   <  2:1434635/139‑1 (MQ=255)

                                                                                      tttACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGcc                                                                                                  >  2:1571581/1‑89 (MQ=255)

                                                                                      tttACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGcc                                                                                                  <  1:1571581/89‑1 (MQ=255)

                                                                                       ttACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATggg                                               <  1:854031/139‑1 (MQ=255)

                                                                                        tACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc                                              <  1:1223584/139‑1 (MQ=255)

                                                                                         aCCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCa                                             <  1:1512843/139‑1 (MQ=255)

                                                                                           cGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAg                                           <  2:102270/139‑1 (MQ=255)

                                                                                            gTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGc                                          >  1:1261819/1‑139 (MQ=255)

                                                                                             tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCt                                         <  2:1349573/139‑1 (MQ=255)

                                                                                                  aaGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAata                                    <  1:1478710/139‑1 (MQ=255)

                                                                                                    gTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAg                                           <  2:771403/130‑1 (MQ=255)

                                                                                                    gTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAg                                           >  1:771403/1‑130 (MQ=255)

                                                                                                       gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAt                                                                                               >  1:1232399/1‑75 (MQ=255)

                                                                                                       gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAt                                                                                               <  2:1232399/75‑1 (MQ=255)

                                                                                                       gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTg                               >  2:544875/1‑139 (MQ=255)

                                                                                                       gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTg                               >  2:407393/1‑139 (MQ=255)

                                                                                                                  cGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTccc                    >  1:1019166/1‑139 (MQ=255)

                                                                                                                  cGATTCAGAACTGGAAGCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTccc                    <  1:723283/139‑1 (MQ=255)

                                                                                                                                  gCCATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGc    <  1:407393/139‑1 (MQ=255)

                                                                                                                                    cATCGAAGGGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg  <  2:1143830/139‑1 (MQ=255)

TTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑GGG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGG  >  NC_000913/3896908‑3897177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A