Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I1 R1	759	65.2	3386260	90.7%	3071337	101.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,897,050	+G	coding (277/666 nt)	yieH →	phosphoenolpyruvate and 6‑phosphogluconate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,897,046	1	.	G	98.7%	265.9 / ‑3.9	74	coding (273/666 nt)	yieH	phosphoenolpyruvate and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (32/41); total (33/41)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AA‑GGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGG  >  NC_000913/3896908‑3897177

tttCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑aa                                                                                                                                      <  1:978576/139‑1 (MQ=255)

     aaGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AA‑ggggg                                                                                                                                <  1:426148/139‑2 (MQ=255)

           tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AA‑ggggg                                                                                                                                <  1:378301/133‑2 (MQ=255)

           tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AA‑ggggg                                                                                                                                >  2:378301/1‑132 (MQ=255)

           tAAAACTGCACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAg                                                                                                                           <  1:1005945/139‑1 (MQ=255)

              aaCTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAgcgc                                                                                                                        <  2:1451046/139‑1 (MQ=255)

                  gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCt                                                                                                                       >  1:452206/1‑136 (MQ=255)

                  gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCt                                                                                                                       <  2:452206/136‑1 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAgc                                                                                                                          >  2:1491615/1‑132 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAgc                                                                                                                          <  1:1491615/132‑1 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTg                                                                                                                   <  1:957003/139‑1 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTg                                                                                                                   <  2:75646/139‑1 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTg                                                                                                                   >  2:957003/1‑139 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTg                                                                                                                   >  2:1175448/1‑139 (MQ=255)

                         aTTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCg                                                                                                             <  2:1377551/139‑1 (MQ=255)

                         aTTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCg                                                                                                             <  1:98933/139‑1 (MQ=255)

                                    tGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGcc                                                                                                  >  2:98842/1‑139 (MQ=255)

                                      tttCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCaa                                                                                                >  2:1222351/1‑139 (MQ=255)

                                       ttCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAt                                                                                               <  1:1222351/139‑1 (MQ=255)

                                        tCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAgcgc                                                                                                                        >  1:1454851/1‑113 (MQ=255)

                                        tCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAgcgc                                                                                                                        <  2:1454851/113‑1 (MQ=255)

                                           cTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    <  1:1445957/130‑1 (MQ=255)

                                           cTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    >  2:1445957/1‑130 (MQ=255)

                                            ttGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                          <  1:98842/139‑1 (MQ=255)

                                            ttGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                          <  1:179017/139‑1 (MQ=255)

                                             tGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtgt                                                                                         >  1:886577/1‑139 (MQ=255)

                                              gAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    <  1:115647/127‑1 (MQ=255)

                                              gAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    >  2:115647/1‑127 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGTAG‑GGGGCTGGAGCGCTCCTg                                                                                                                   >  1:773329/1‑106 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑TAGGGGGCTGGAGCGCTCCTg                                                                                                                   <  2:773329/106‑1 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGa                                                                                                                            >  1:1013989/1‑97 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGa                                                                                                                            <  2:1013989/97‑1 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGc                                                                                                              >  2:1494886/1‑111 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGc                                                                                                              <  1:1494886/111‑1 (MQ=255)

                                                         tACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACgg                                                                             >  2:650839/1‑139 (MQ=255)

                                                              tAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa                                                                                                 >  1:379694/1‑114 (MQ=255)

                                                              tAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa                                                                                                 <  2:379694/114‑1 (MQ=255)

                                                              tAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCaa                                                                        >  2:235956/1‑139 (MQ=255)

                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCt                                                                                                                       <  2:1256814/91‑1 (MQ=255)

                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCt                                                                                                                       >  1:1256814/1‑91 (MQ=255)

                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTcc                                                                                                                     >  2:1571739/1‑93 (MQ=255)

                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTcc                                                                                                                     <  1:1571739/93‑1 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                          <  1:1366396/119‑1 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                          >  2:1366396/1‑119 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTa                                                                                 >  1:153145/1‑128 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTa                                                                                 <  2:153145/128‑1 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACg                                                                              <  1:1419594/131‑1 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACg                                                                              >  2:1419594/1‑131 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAAt                                                                      >  1:129268/1‑139 (MQ=255)

                                                                 cGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGc                                                                            >  2:839274/1‑132 (MQ=255)

                                                                 cGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGc                                                                            <  1:839274/132‑1 (MQ=255)

                                                                    aaaCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACa                                                                  <  1:650839/139‑1 (MQ=255)

                                                                      aCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACaaa                                                                <  2:1060443/139‑1 (MQ=255)

                                                                                            gTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGt                                                                                      <  1:250864/95‑1 (MQ=255)

                                                                                            gTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGt                                                                                      >  2:250864/1‑95 (MQ=255)

                                                                                               cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGc                                                                                                              <  1:743195/68‑1 (MQ=255)

                                                                                               cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGc                                                                                                              >  2:1016639/1‑68 (MQ=255)

                                                                                               cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGc                                                                                                              >  2:743195/1‑68 (MQ=255)

                                                                                               cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGc                                                                                                              <  1:1016639/68‑1 (MQ=255)

                                                                                               cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc                                              <  2:1653237/132‑1 (MQ=255)

                                                                                               cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc                                              >  1:1653237/1‑132 (MQ=255)

                                                                                               cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGa                                       <  1:317496/139‑1 (MQ=255)

                                                                                                  aaGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAata                                    <  1:235956/139‑1 (MQ=255)

                                                                                                    gTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑aa                                                                                                                                      >  1:745239/1‑39 (MQ=255)

                                                                                                    gTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑aa                                                                                                                                      <  2:745239/39‑1 (MQ=255)

                                                                                                    gTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTg                                                                                                                   <  1:879728/58‑1 (MQ=255)

                                                                                                    gTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTg                                                                                                                   >  2:879728/1‑58 (MQ=255)

                                                                                                       gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCTATCACTGCGCCAATGTGTGTGGTa                                                                                     <  2:1146452/85‑1 (MQ=255)

                                                                                                       gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTa                                                                                     >  1:1146452/1‑85 (MQ=255)

                                                                                                       gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AA‑ggg                                                                                                                                  <  2:170079/39‑1 (MQ=255)

                                                                                                       gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AA‑ggg                                                                                                                                  >  1:170079/1‑39 (MQ=255)

                                                                                                            gCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                          <  2:411837/75‑1 (MQ=255)

                                                                                                            gCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                          >  1:411837/1‑75 (MQ=255)

                                                                                                            gCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCacta                          >  1:1367447/1‑139 (MQ=255)

                                                                                                               gTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACtt                       <  2:1367447/139‑1 (MQ=255)

                                                                                                               gTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACtt                       <  2:886577/139‑1 (MQ=255)

                                                                                                                          aaCTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAAc            >  2:629057/1‑139 (MQ=255)

                                                                                                                               gAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACaaa                                                                >  1:815785/1‑82 (MQ=255)

                                                                                                                               gAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACaaa                                                                <  2:815785/82‑1 (MQ=255)

                                                                                                                                aaGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCa      <  1:1175448/139‑1 (MQ=255)

                                                                                                                                    cATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg  <  1:629057/139‑1 (MQ=255)

                                                                                                                                    cATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCACCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg  <  1:890869/139‑1 (MQ=255)

TTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AA‑GGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGG  >  NC_000913/3896908‑3897177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A