Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	765	53.2	2691310	93.4%	2513683	103.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,897,050	+G	coding (277/666 nt)	yieH →	phosphoenolpyruvate and 6‑phosphogluconate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,897,046	1	.	G	96.6%	203.5 / ‑0.7	59	coding (273/666 nt)	yieH	phosphoenolpyruvate and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (30/27); total (30/29)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑G‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGG  >  NC_000913/3896908‑3897177

tttCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                        <  1:436864/139‑1 (MQ=255)

      aGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑g‑gg‑ggc                                                                                                                               <  1:368772/139‑3 (MQ=255)

             aaaCTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAgcg                                                                                                                         <  1:133082/139‑1 (MQ=255)

                   tACGAAATTATCGCTATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTg                                                                                                                   >  2:1170357/1‑139 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTg                                                                                                                   >  1:458727/1‑139 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTg                                                                                                                   <  2:273506/139‑1 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTg                                                                                                                   >  2:1230456/1‑139 (MQ=255)

                         aTTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                        <  1:1026441/114‑1 (MQ=255)

                         aTTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                        >  2:1026441/1‑114 (MQ=255)

                                  atTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCGATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    <  1:820385/139‑1 (MQ=255)

                                        tCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtg                                                                                              <  2:200094/139‑1 (MQ=255)

                                             tGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAgc                                                                                                                          <  2:453488/106‑1 (MQ=255)

                                             tGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAgc                                                                                                                          >  1:453488/1‑106 (MQ=255)

                                              gAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtgtg                                                                                        >  1:884675/1‑139 (MQ=255)

                                               aaCATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                        >  2:1229929/1‑92 (MQ=255)

                                               aaCATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                        <  1:1229929/92‑1 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                        >  1:800570/1‑87 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                        <  2:800570/87‑1 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                          >  2:654777/1‑131 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                          >  2:582006/1‑131 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                          <  1:582006/131‑1 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                          <  1:654777/131‑1 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGt                                                                                      >  2:269456/1‑135 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGt                                                                                      <  1:269456/135‑1 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCt                                                                                  >  1:1337876/1‑139 (MQ=255)

                                                         tACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACgg                                                                             >  1:477159/1‑139 (MQ=255)

                                                          aCGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGAt                                                                                                           >  2:54181/1‑108 (MQ=255)

                                                          aCGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGAt                                                                                                           <  1:54181/108‑1 (MQ=255)

                                                          aCGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGc                                                                            <  2:458727/139‑1 (MQ=255)

                                                              tAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCaa                                                                        >  1:1158409/1‑139 (MQ=255)

                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTc                                                                                                                      >  2:1282464/1‑92 (MQ=255)

                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTc                                                                                                                      <  1:1282464/92‑1 (MQ=255)

                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTg                                                                                                                   <  1:452714/95‑1 (MQ=255)

                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTg                                                                                                                   >  2:452714/1‑95 (MQ=255)

                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa                                                                                                 <  1:570025/113‑1 (MQ=255)

                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa                                                                                                 >  2:570025/1‑113 (MQ=255)

                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGACTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGcc                                                                                                  <  1:724259/112‑1 (MQ=255)

                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGACTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGcc                                                                                                  >  2:724259/1‑112 (MQ=255)

                                                                     aaCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                          >  1:1005089/1‑114 (MQ=255)

                                                                     aaCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                          <  2:1005089/114‑1 (MQ=255)

                                                                        aGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAAt                                                              >  1:442630/1‑139 (MQ=255)

                                                                          aaGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgc                                                            >  1:563727/1‑139 (MQ=255)

                                                                            gCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcag                                                          <  2:367144/139‑1 (MQ=255)

                                                                                aaCACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCAtt                                                      >  2:338714/1‑139 (MQ=255)

                                                                                        tACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAAGTGGAAGCCATCGAA‑G‑GGAGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc                                              <  2:884675/139‑1 (MQ=255)

                                                                                        tACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc                                              <  1:1230456/139‑1 (MQ=255)

                                                                                        tACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc                                              <  2:1337876/139‑1 (MQ=255)

                                                                                          ccGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑GTGG‑GCTGGAGCGCTCATGTCAGCGATCACTGCGCCAATGTGTGTGTTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATTGGCaa                                            <  2:477159/139‑1 (MQ=255)

                                                                                             tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgt                                                                                             >  2:544431/1‑87 (MQ=255)

                                                                                             tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgt                                                                                             <  1:544431/87‑1 (MQ=255)

                                                                                             tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCt                                         >  1:417765/1‑139 (MQ=255)

                                                                                                  aaGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAata                                    <  2:1158409/139‑1 (MQ=255)

                                                                                                       gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTg                               >  1:1247234/1‑139 (MQ=255)

                                                                                                         tCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATg                                                             <  2:526731/107‑1 (MQ=255)

                                                                                                         tCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATg                                                             >  1:526731/1‑107 (MQ=255)

                                                                                                                ttCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTg                                                                                                                   <  1:1223397/46‑1 (MQ=255)

                                                                                                                ttCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTg                                                                                                                   >  2:1223397/1‑46 (MQ=39)

                                                                                                                ttCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTc                      >  2:1158231/1‑139 (MQ=255)

                                                                                                                       cAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATa               >  2:48243/1‑139 (MQ=255)

                                                                                                                               gAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    <  1:994558/46‑1 (MQ=255)

                                                                                                                               gAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcag                                                          <  2:1043391/88‑1 (MQ=255)

                                                                                                                               gAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcag                                                          >  1:1043391/1‑88 (MQ=255)

                                                                                                                               gAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTc       >  2:228569/1‑139 (MQ=255)

                                                                                                                               gAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGCCAGCGATCACTgcg                                                                                                    >  2:994558/1‑46 (MQ=255)

                                                                                                                                  gCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGc    <  1:48243/139‑1 (MQ=255)

                                                                                                                                    cATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg  <  2:442630/139‑1 (MQ=255)

                                                                                                                                    cATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCga  <  1:228569/139‑2 (MQ=255)

TTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑G‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGG  >  NC_000913/3896908‑3897177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A