Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,897,050	+G	coding (277/666 nt)	yieH →	phosphoenolpyruvate and 6‑phosphogluconate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,897,046	1	.	G	96.4%	196.4 / ‑0.2	56	coding (273/666 nt)	yieH	phosphoenolpyruvate and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (28/26); total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑G‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCA  >  NC_000913/3896911‑3897173

cAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑g‑gg                                                                                                                               <  2:922674/139‑1 (MQ=255)

   aGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑g‑gg‑ggc                                                                                                                           <  2:902857/139‑3 (MQ=255)

        tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAg                                                                                                                       >  2:973587/1‑139 (MQ=255)

             cTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTc                                                                                                                  <  1:959572/139‑1 (MQ=255)

               gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCt                                                                                                                >  2:739405/1‑139 (MQ=255)

               gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCt                                                                                                                >  1:288156/1‑139 (MQ=255)

                        tATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGAt                                                                                                       >  1:101928/1‑139 (MQ=255)

                          tCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCa                                                                                                     >  1:583510/1‑139 (MQ=255)

                          tCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCa                                                                                                     >  2:620136/1‑139 (MQ=255)

                            gATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACt                                                                                                   <  1:227840/139‑1 (MQ=255)

                                 tGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                    <  2:162179/103‑1 (MQ=255)

                                 tGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                    >  1:162179/1‑103 (MQ=255)

                                 tGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGcc                                                                                              >  1:938384/1‑139 (MQ=255)

                                  gTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa                                                                                             <  1:739405/139‑1 (MQ=255)

                                     tCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtg                                                                                          >  1:609405/1‑139 (MQ=255)

                                     tCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtg                                                                                          <  2:583510/139‑1 (MQ=255)

                                        cTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgt                                                                                       >  1:172183/1‑139 (MQ=255)

                                         ttGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                      <  2:288156/139‑1 (MQ=255)

                                         ttGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                      <  1:620136/139‑1 (MQ=255)

                                           gAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGa                                                                                                                        >  1:474544/1‑103 (MQ=255)

                                           gAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGa                                                                                                                        <  2:474544/103‑1 (MQ=255)

                                                      tACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                    <  2:920560/82‑1 (MQ=255)

                                                      tACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                    >  1:920560/1‑82 (MQ=255)

                                                      tACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACgg                                                                         >  1:940194/1‑139 (MQ=255)

                                                      tACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACgg                                                                         >  2:875512/1‑139 (MQ=255)

                                                       aCGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGc                                                                        <  2:885132/139‑1 (MQ=255)

                                                           tAGCGTAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                >  1:696439/1‑111 (MQ=255)

                                                           tAGCGTAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                <  2:696439/111‑1 (MQ=255)

                                                           tAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTg                                                                                                  >  1:328297/1‑109 (MQ=255)

                                                           tAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTg                                                                                                  <  2:328297/109‑1 (MQ=255)

                                                           tAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCaa                                                                    >  1:660847/1‑139 (MQ=255)

                                                            aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCaaa                                                                   >  2:594058/1‑139 (MQ=255)

                                                             gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTgc                                                                                                 >  2:614033/1‑108 (MQ=255)

                                                             gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTgc                                                                                                 <  1:614033/108‑1 (MQ=255)

                                                             gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGc                                                                        <  1:973891/133‑1 (MQ=255)

                                                             gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGc                                                                        >  2:973891/1‑133 (MQ=255)

                                                             gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAAt                                                                  >  2:687735/1‑139 (MQ=255)

                                                                         gCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcag                                                      <  2:495727/139‑1 (MQ=255)

                                                                             aaCACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGcc                                                                                              <  2:786938/95‑1 (MQ=255)

                                                                             aaCACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGcc                                                                                              >  1:786938/1‑95 (MQ=255)

                                                                              acacGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTc                                                 <  1:594058/139‑1 (MQ=255)

                                                                               cacGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCt                                                <  2:132670/139‑1 (MQ=255)

                                                                                   tttACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATgg                                            <  1:248932/139‑1 (MQ=255)

                                                                                    ttACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑G‑GGAGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATggg                                           <  1:560862/139‑1 (MQ=255)

                                                                                     tACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc                                          <  2:322414/139‑1 (MQ=255)

                                                                                         gTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGc                                      >  1:914270/1‑139 (MQ=255)

                                                                                          tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑g                                                                                                                                  >  1:145934/1‑47 (MQ=255)

                                                                                          tGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑g                                                                                                                                  <  2:145934/47‑1 (MQ=255)

                                                                                            cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGa                                   >  2:97487/1‑139 (MQ=255)

                                                                                            cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGa                                   <  1:97487/139‑1 (MQ=255)

                                                                                            cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGa                                   <  2:266377/139‑1 (MQ=255)

                                                                                            cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGa                                   >  2:241679/1‑139 (MQ=255)

                                                                                            cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGa                                   <  2:830534/139‑1 (MQ=255)

                                                                                            cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGa                                   >  2:863422/1‑139 (MQ=255)

                                                                                                    gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                    <  2:387779/36‑1 (MQ=255)

                                                                                                    gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                    >  1:387779/1‑36 (MQ=255)

                                                                                                    gCTCGGCTGTTCGAGTCAGAACTGGAAGCCATCGAA‑GAGG‑GCTGGAGCCCTCCTGTCAGCGATCACCGCGCCAACGTGTGTGGTATCTATCGGCCCAAATAACTAAATGCAGCAATCGATGGGCAAGCTGAATATGTTg                           >  2:316393/1‑139 (MQ=255)

                                                                                                            gTTCGATTGAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGTGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACtt                   <  2:645697/139‑1 (MQ=255)

                                                                                                            gTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcag                                                      <  2:17655/104‑1 (MQ=255)

                                                                                                            gTTCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcag                                                      >  1:17655/1‑104 (MQ=255)

                                                                                                              tCGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTcc                 <  2:940194/139‑1 (MQ=255)

                                                                                                               cGATTCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTccc                <  1:241679/139‑1 (MQ=255)

                                                                                                                  ttCAGAACTGGAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGa             >  1:912316/1‑139 (MQ=255)

                                                                                                                            gAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTc   >  2:875102/1‑139 (MQ=255)

                                                                                                                            gAAGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTc   >  2:595166/1‑139 (MQ=255)

                                                                                                                             aaGCCATCGAAGG‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCa  <  1:875512/139‑1 (MQ=255)

CAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑G‑GG‑GCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCA  >  NC_000913/3896911‑3897173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A