Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	750	51.0	2595496	93.6%	2429384	106.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,897,050	+G	coding (277/666 nt)	yieH →	phosphoenolpyruvate and 6‑phosphogluconate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,897,046	1	.	G	98.6%	255.6 / ‑3.6	73	coding (273/666 nt)	yieH	phosphoenolpyruvate and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (38/34); total (38/35)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

TTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AA‑GGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGG  >  NC_000913/3896908‑3897177

tttCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑aa                                                                                                                                      >  2:422177/1‑139 (MQ=255)

tttCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑aa                                                                                                                                      >  2:236307/1‑139 (MQ=255)

tttCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑aa                                                                                                                                      <  2:559469/139‑1 (MQ=255)

   cAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AA‑ggg                                                                                                                                  <  2:1285018/139‑1 (MQ=255)

           tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAg                                                                                                                           >  2:840170/1‑139 (MQ=255)

           tAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAg                                                                                                                           >  2:580073/1‑139 (MQ=255)

                  gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAGCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AA‑g                                                                                                                                    >  2:1209218/1‑122 (MQ=255)

                  gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCt                                                                                                                    >  1:692202/1‑139 (MQ=255)

                  gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AA‑gggggctg                                                                                                                             <  1:939913/129‑5 (MQ=255)

                  gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AA‑gggggctg                                                                                                                             >  2:939913/1‑125 (MQ=255)

                  gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AA‑g                                                                                                                                    <  1:1209218/122‑1 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTcc                                                                                                                     >  2:262968/1‑137 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTcc                                                                                                                     <  1:262968/137‑1 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTg                                                                                                                   >  1:859993/1‑139 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTg                                                                                                                   >  1:880884/1‑139 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTg                                                                                                                   >  2:1177218/1‑139 (MQ=255)

                   tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTg                                                                                                                   >  1:1204427/1‑139 (MQ=255)

                        aaTTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGc                                                                                                              >  2:1081201/1‑139 (MQ=255)

                               gATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACt                                                                                                       <  2:1260837/139‑1 (MQ=255)

                                  atTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGTAG‑GGGGCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    <  1:126829/139‑1 (MQ=255)

                                  atTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    >  2:126829/1‑139 (MQ=255)

                                  atTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    <  2:1204427/139‑1 (MQ=255)

                                    tGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGcc                                                                                                  >  2:1245185/1‑139 (MQ=255)

                                     gTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa                                                                                                 <  1:236307/139‑1 (MQ=255)

                                     gTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa                                                                                                 <  2:880884/139‑1 (MQ=255)

                                     gTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa                                                                                                 <  1:498773/139‑1 (MQ=255)

                                         cccTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgt                                                                                             <  1:1081201/139‑1 (MQ=255)

                                           cTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCt                                                                                                                       >  2:131392/1‑111 (MQ=255)

                                           cTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCt                                                                                                                       <  1:131392/111‑1 (MQ=255)

                                           cTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgt                                                                                           >  1:1169914/1‑139 (MQ=255)

                                           cTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtg                                                                                              <  2:509515/136‑1 (MQ=255)

                                           cTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtg                                                                                              >  1:509515/1‑136 (MQ=255)

                                             tGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGATCGATTCAGAACTGGACGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgggt                                                                                         >  2:1273445/1‑139 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCt                                                                                                                       >  2:970240/1‑102 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCt                                                                                                                       <  1:970240/102‑1 (MQ=255)

                                                    ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCt                                                                                  >  2:408842/1‑139 (MQ=255)

                                                      tgtTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTaa                                                                                <  1:1273445/139‑1 (MQ=255)

                                                          aCGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGc                                                                            <  1:934039/139‑1 (MQ=255)

                                                          aCGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGc                                                                            <  1:580073/139‑1 (MQ=255)

                                                           cGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCaa                                                                                                <  2:929959/118‑1 (MQ=255)

                                                           cGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCaa                                                                                                >  1:929959/1‑118 (MQ=255)

                                                           cGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGcc                                                                           >  1:271779/1‑139 (MQ=255)

                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    >  1:811561/1‑110 (MQ=255)

                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    <  2:811561/110‑1 (MQ=255)

                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCaaa                                                                       >  1:1051233/1‑139 (MQ=255)

                                                               aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCaaa                                                                       >  2:1157703/1‑139 (MQ=255)

                                                                gCGAAAACAGAAGCTGGACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AA‑gggggctg                                                                                                                             >  2:9669/1‑79 (MQ=255)

                                                                gCGAAAACAGAAGCTGGACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AA‑gggggctg                                                                                                                             <  1:9669/83‑5 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCa                                                                                                         <  2:16230/104‑1 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCa                                                                                                         >  1:16230/1‑104 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    <  2:706753/109‑1 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    >  1:706753/1‑109 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAt                                                                                               <  2:880045/114‑1 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAAt                                                                      >  1:560471/1‑139 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AA‑gggggctg                                                                                                                             <  2:955270/83‑5 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AA‑gggggctg                                                                                                                             >  1:955270/1‑79 (MQ=255)

                                                                gCGAAAACAGAAGCTGAACAAGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAt                                                                                               >  1:880045/1‑114 (MQ=255)

                                                                     aaCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCa                                                                                                         <  1:984034/99‑1 (MQ=255)

                                                                     aaCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCa                                                                                                         >  2:984034/1‑99 (MQ=255)

                                                                              tGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcagca                                                        <  1:840170/139‑1 (MQ=255)

                                                                                       ttACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATggg                                               <  2:271779/139‑1 (MQ=255)

                                                                                       ttACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATggg                                               >  2:514078/1‑139 (MQ=255)

                                                                                        tACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc                                              <  2:222870/139‑1 (MQ=255)

                                                                                               cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGa                                       >  2:843581/1‑139 (MQ=255)

                                                                                                    gTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCg                                                                                                             >  2:659742/1‑64 (MQ=255)

                                                                                                    gTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCg                                                                                                             <  1:659742/64‑1 (MQ=255)

                                                                                                       gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGt                                 >  2:1036921/1‑137 (MQ=255)

                                                                                                       gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGt                                 <  1:1036921/137‑1 (MQ=255)

                                                                                                       gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTg                               <  2:692202/139‑1 (MQ=255)

                                                                                                        cTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGc                              <  1:1114391/139‑1 (MQ=255)

                                                                                                              tGTTCGATTCTGAACTGGAAGCAATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCa                                                                                                         >  2:631277/1‑58 (MQ=37)

                                                                                                              tGTTCGATTCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCa                                                                                                         <  1:631277/58‑1 (MQ=255)

                                                                                                                     ttCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACa                                                                  >  2:20987/1‑90 (MQ=255)

                                                                                                                     ttCAGAACTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACa                                                                  <  1:20987/90‑1 (MQ=255)

                                                                                                                          aaCTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAt                                                                                               >  2:207928/1‑56 (MQ=255)

                                                                                                                          aaCTGGAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAt                                                                                               <  1:207928/56‑1 (MQ=255)

                                                                                                                               gAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTc                                                     <  1:1038634/93‑1 (MQ=255)

                                                                                                                               gAAGCCATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTc                                                     >  2:1038634/1‑93 (MQ=255)

                                                                                                                                    cATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCa                                                                                                         >  1:141181/1‑36 (MQ=255)

                                                                                                                                    cATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCa                                                                                                         <  2:141181/36‑1 (MQ=255)

                                                                                                                                    cATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTa                                                                                 <  1:33200/60‑1 (MQ=255)

                                                                                                                                    cATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTa                                                                                 >  2:33200/1‑60 (MQ=255)

                                                                                                                                    cATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg  <  1:843581/139‑1 (MQ=255)

                                                                                                                                    cATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg  <  1:422177/139‑1 (MQ=255)

                                                                                                                                    cATCG‑AAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg  >  1:965707/1‑139 (MQ=255)

TTTCAAAGGTGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCG‑AA‑GGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGG  >  NC_000913/3896908‑3897177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A