Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	771	44.8	2153074	94.1%	2026042	109.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,732,319	G→A	G40E (GGG→GAG)	xylG →	D‑xylose ABC transporter dual domain ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,732,319	0	G	A	100.0%	159.9 / NA	52	G40E (GGG→GAG)	xylG	D‑xylose ABC transporter dual domain ATPase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (26/26); total (26/26)

GGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGGGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCA  >  NC_000913/3732183‑3732441

ggCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGt                                                                                                                          >  2:573093/1‑139 (MQ=255)

           aGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCa                                                                                                               >  1:393703/1‑139 (MQ=255)

           aGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCa                                                                                                               >  1:181420/1‑139 (MQ=255)

                ttATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTACGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAAc                                                                                                             >  1:923040/1‑136 (MQ=255)

                ttATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTACGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAAc                                                                                                             <  2:923040/136‑1 (MQ=255)

                       ttATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGaaa                                                                                                   <  2:393703/139‑1 (MQ=255)

                        tATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAg                                                                                                  >  1:511863/1‑139 (MQ=255)

                          tCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                <  2:672456/139‑1 (MQ=255)

                          tCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                <  2:260404/139‑1 (MQ=255)

                            tACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                              <  2:706281/139‑1 (MQ=255)

                            tACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                              <  2:181420/139‑1 (MQ=255)

                             aCTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtg                                                                                             <  2:456708/139‑1 (MQ=255)

                               ttGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                <  1:268804/134‑1 (MQ=255)

                               ttGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                >  2:268804/1‑134 (MQ=255)

                               ttGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtg                                                                                           <  2:511863/139‑1 (MQ=255)

                               ttGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtg                                                                                           >  2:332678/1‑139 (MQ=255)

                                 gAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtgtg                                                                                         <  2:603165/139‑1 (MQ=255)

                                   aaTGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACATCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTTCTGTGTGGt                                                                                       >  1:1012256/1‑139 (MQ=255)

                                    aTGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTa                                                                                      >  1:267113/1‑139 (MQ=255)

                                    aTGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTa                                                                                      <  1:573093/139‑1 (MQ=255)

                                    aTGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGATGTGTGGTa                                                                                      >  2:634002/1‑139 (MQ=255)

                                       aagaaCATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTAttt                                                                                   <  2:740917/139‑1 (MQ=255)

                                          aaCATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATc                                                                                <  2:267113/139‑1 (MQ=255)

                                              ttACCACACCCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCa                                                                            <  2:254080/139‑1 (MQ=255)

                                                          tCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGa                                                                >  1:331947/1‑139 (MQ=255)

                                                           cGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtgt                                                                                          >  1:87245/1‑112 (MQ=255)

                                                           cGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtgt                                                                                          <  2:87245/112‑1 (MQ=255)

                                                           cGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGaa                                                               >  2:746118/1‑139 (MQ=255)

                                                                   tgAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAat                                                       >  2:682488/1‑139 (MQ=255)

                                                                                      cTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCa                                                                            >  2:100536/1‑99 (MQ=255)

                                                                                      cTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCa                                                                            <  1:100536/99‑1 (MQ=255)

                                                                                      cTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACGATGTGGGGAAAGTGAGTCTGGAAAATCAAAGCTGATCAAGGTGCTGAGTGGTATTTATCGCCATGGCTCCTCCGAAGGCCAAATTATGTTTTCTGGAGCagag                                    >  2:997418/1‑139 (MQ=255)

                                                                                         cTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                <  1:687066/76‑1 (MQ=255)

                                                                                         cTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                >  2:687066/1‑76 (MQ=255)

                                                                                         cTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                              >  1:464777/1‑78 (MQ=255)

                                                                                         cTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                              <  2:464777/78‑1 (MQ=255)

                                                                                         cTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTAtttt                                                 >  2:335349/1‑123 (MQ=255)

                                                                                         cTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTAtttt                                                 <  1:335349/123‑1 (MQ=255)

                                                                                             cGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAgg                             <  1:573203/139‑1 (MQ=255)

                                                                                                   aaTGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTc                       <  1:471335/139‑1 (MQ=255)

                                                                                                   aaTGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTc                       >  1:813120/1‑139 (MQ=255)

                                                                                                     tGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTcac                     <  1:634002/139‑1 (MQ=255)

                                                                                                       cTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACAt                   >  2:609037/1‑139 (MQ=255)

                                                                                                          gCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAgg                             >  1:364687/1‑126 (MQ=255)

                                                                                                          gCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAgg                             <  2:364687/126‑1 (MQ=255)

                                                                                                          gCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCcg                >  2:937883/1‑139 (MQ=255)

                                                                                                             aaaTCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                <  2:835960/56‑1 (MQ=255)

                                                                                                             aaaTCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                >  1:835960/1‑56 (MQ=255)

                                                                                                              aaTCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGAt            >  1:7337/1‑139 (MQ=255)

                                                                                                                        cTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCa  <  2:813120/139‑1 (MQ=255)

                                                                                                                          ttGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTgg                                                                                        >  1:1075519/1‑51 (MQ=255)

                                                                                                                          ttGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTgg                                                                                        <  2:1075519/51‑1 (MQ=255)

GGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGGGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCA  >  NC_000913/3732183‑3732441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A