Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,732,319	G→A	G40E (GGG→GAG)	xylG →	D‑xylose ABC transporter dual domain ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,732,319	0	G	A	100.0%	141.5 / NA	45	G40E (GGG→GAG)	xylG	D‑xylose ABC transporter dual domain ATPase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/24); total (21/24)

GGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGGGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCAAAGGTAT  >  NC_000913/3732183‑3732448

ggCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGt                                                                                                                                 <  2:327172/139‑1 (MQ=255)

   ttAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTg                                                                                                                              >  2:219948/1‑139 (MQ=255)

           aGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCa                                                                                                                      >  1:666379/1‑139 (MQ=255)

                ttATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCt                                                                                                                 >  1:997070/1‑139 (MQ=255)

                ttATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCt                                                                                                                 >  1:334091/1‑139 (MQ=255)

                    gCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCtgatg                                                                                                             >  1:1111901/1‑139 (MQ=255)

                     tcTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCtgatga                                                                                                            <  2:666379/138‑1 (MQ=255)

                     ccTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCtgatga                                                                                                            <  2:102693/139‑1 (MQ=255)

                         aTCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGt                                                                                                        >  2:189190/1‑139 (MQ=255)

                          tCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                       <  2:997070/139‑1 (MQ=255)

                            tACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                     <  1:258884/139‑1 (MQ=255)

                              cTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgt                                                                                                   >  2:687403/1‑139 (MQ=255)

                               ttGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtg                                                                                                  <  2:334091/139‑1 (MQ=255)

                               ttGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                     >  1:669265/1‑136 (MQ=255)

                               ttGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                     <  2:669265/136‑1 (MQ=255)

                                  aaaTGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTgg                                                                                               <  2:221569/139‑1 (MQ=255)

                                  aaaTGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTgg                                                                                               >  2:572132/1‑139 (MQ=255)

                                    aTGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTa                                                                                             >  2:111886/1‑139 (MQ=255)

                                       aagaaCATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTAttt                                                                                          <  1:219948/139‑1 (MQ=255)

                                                         ttCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACg                                                                        >  1:852412/1‑139 (MQ=255)

                                                          tCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGa                                                                       >  1:372099/1‑139 (MQ=255)

                                                            ggCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAg                                                                     <  1:687403/139‑1 (MQ=255)

                                                                gtgtGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGa                                                                 <  1:189190/139‑1 (MQ=255)

                                                                 tgtgAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACg                                                                                                                   >  2:1145416/1‑88 (MQ=255)

                                                                 tgtgAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACg                                                                                                                   <  1:1145416/88‑1 (MQ=255)

                                                                 tgtgAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                     <  1:18078/102‑1 (MQ=255)

                                                                 tgtgAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                     >  2:1304964/1‑102 (MQ=255)

                                                                 tgtgAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                     >  2:18078/1‑102 (MQ=255)

                                                                 tgtgAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                     <  1:1304964/102‑1 (MQ=255)

                                                                           aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTg                                                      >  2:1043408/1‑139 (MQ=255)

                                                                           aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTg                                                      <  2:158132/139‑1 (MQ=255)

                                                                               aTAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCggg                                                  <  2:1058296/139‑1 (MQ=255)

                                                                                    gTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGagaag                                             <  2:1111901/139‑1 (MQ=255)

                                                                                              ggTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtgt                                                                                                 <  1:314364/77‑1 (MQ=255)

                                                                                              ggTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtgt                                                                                                 >  2:314364/1‑77 (MQ=255)

                                                                                              ggTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGc                                   >  1:62009/1‑139 (MQ=255)

                                                                                                 tGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAg                                <  1:1043408/139‑1 (MQ=255)

                                                                                                   aaTGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAAc                                                                                                                    <  1:114673/53‑1 (MQ=255)

                                                                                                   aaTGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAAc                                                                                                                    >  2:114673/1‑53 (MQ=255)

                                                                                                          gCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                     <  1:120863/61‑1 (MQ=255)

                                                                                                          gCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                     >  2:120863/1‑61 (MQ=255)

                                                                                                                 cGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATAcc                <  1:572132/139‑1 (MQ=255)

                                                                                                                               gggAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCAAAGGTAt  <  2:852412/139‑1 (MQ=255)

                                                                                                                                 gAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtgt                                                                                                 <  2:477447/42‑1 (MQ=255)

                                                                                                                                 gAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtgt                                                                                                 >  1:477447/1‑42 (MQ=255)

GGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGGGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCAAAGGTAT  >  NC_000913/3732183‑3732448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A