Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I2 R1 764 42.0 2018750 95.5% 1927906 109.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,732,319 G→A G40E (GGG→GAG)  xylG → D‑xylose ABC transporter dual domain ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,732,3190GA100.0% 300.4 / NA 92G40E (GGG→GAG) xylGD‑xylose ABC transporter dual domain ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (47/45);  total (47/45)

GGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGGGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCAAAGGTATCGCC  >  NC_000913/3732183‑3732452
                                                                                                                                        |                                                                                                                                     
ggCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGt                                                                                                                                     <  1:1008143/139‑1 (MQ=255)
    tAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTgg                                                                                                                                 <  1:79307/139‑1 (MQ=255)
     aGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGt                                                                                                                                <  2:901024/139‑1 (MQ=255)
           aGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCa                                                                                                                          >  1:450206/1‑139 (MQ=255)
           aGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCa                                                                                                                          >  2:111585/1‑139 (MQ=255)
           aGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCa                                                                                                                          >  2:123792/1‑139 (MQ=255)
           aGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCa                                                                                                                          >  2:227588/1‑139 (MQ=255)
               gTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGc                                                                                                                      >  2:584462/1‑139 (MQ=255)
                ttATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCt                                                                                                                     >  2:174186/1‑139 (MQ=255)
                ttATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCt                                                                                                                     <  1:535508/139‑1 (MQ=255)
                  aTGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCtga                                                                                                                   >  2:196308/1‑139 (MQ=255)
                       ttATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGaaa                                                                                                              <  2:892280/139‑1 (MQ=255)
                         aTCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGt                                                                                                            >  1:276607/1‑139 (MQ=255)
                         aTCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGt                                                                                                            >  2:827170/1‑139 (MQ=255)
                         aTCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGt                                                                                                            >  1:820470/1‑139 (MQ=255)
                          tCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                           <  1:827170/139‑1 (MQ=255)
                          tCTACTGGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATATCGTCTGCTTGCGGTTGAATGGTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                           <  2:905409/139‑1 (MQ=255)
                            tACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                         <  1:584462/139‑1 (MQ=255)
                               ttGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtg                                                                                                      <  1:227588/139‑1 (MQ=255)
                                tGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtgt                                                                                                     <  2:276607/139‑1 (MQ=255)
                                tGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtgt                                                                                                     <  1:111585/139‑1 (MQ=255)
                                tGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtgt                                                                                                     >  1:32441/1‑139 (MQ=255)
                                tGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtgt                                                                                                     <  1:123792/139‑1 (MQ=255)
                                  aaaTGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                         <  1:875192/133‑1 (MQ=255)
                                  aaaTGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                         >  2:875192/1‑133 (MQ=255)
                                    aTGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTa                                                                                                 >  2:960210/1‑139 (MQ=255)
                                    aTGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTa                                                                                                 >  1:267962/1‑139 (MQ=255)
                                        agaaCATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTa                                                                                             <  1:960210/139‑1 (MQ=255)
                                            cATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATccc                                                                                         >  2:134031/1‑139 (MQ=255)
                                              ttACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCa                                                                                       <  2:820470/139‑1 (MQ=255)
                                               tACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAAt                                                                                                                            <  2:141194/101‑1 (MQ=255)
                                               tACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAAt                                                                                                                            >  1:141194/1‑101 (MQ=255)
                                               tACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                         >  1:750189/1‑120 (MQ=255)
                                               tACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                         <  2:750189/120‑1 (MQ=255)
                                               tACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCAt                                                                                      >  1:841262/1‑139 (MQ=255)
                                                 ccAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATgg                                                                                    >  2:229911/1‑139 (MQ=255)
                                                     aaCCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                           >  2:445872/1‑112 (MQ=255)
                                                     aaCCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                           <  1:445872/112‑1 (MQ=255)
                                                             gCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAgg                                                                        <  2:450206/139‑1 (MQ=255)
                                                                 tgtgAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtg                                                                                                      >  2:871433/1‑105 (MQ=255)
                                                                 tgtgAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtg                                                                                                      <  1:871433/105‑1 (MQ=255)
                                                                 tgtgAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGaa                                                                    >  1:643250/1‑139 (MQ=255)
                                                                   tgAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAat                                                                  >  2:91257/1‑139 (MQ=255)
                                                                           aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                         >  1:833838/1‑92 (MQ=255)
                                                                           aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                         <  2:833838/92‑1 (MQ=255)
                                                                           aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGc                                                                                   >  1:695445/1‑114 (MQ=255)
                                                                           aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGc                                                                                   <  2:695445/114‑1 (MQ=255)
                                                                           aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAg                                                                         <  2:414140/124‑1 (MQ=255)
                                                                           aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAg                                                                         >  1:414140/1‑124 (MQ=255)
                                                                           aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTg                                                          >  1:635426/1‑139 (MQ=255)
                                                                           aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTg                                                          <  2:841262/139‑1 (MQ=255)
                                                                           aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTg                                                          <  2:643250/139‑1 (MQ=255)
                                                                             tGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGc                                                                                   <  1:29653/112‑1 (MQ=255)
                                                                             tGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGc                                                                                   >  2:29653/1‑112 (MQ=255)
                                                                                  aCGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGgaga                                                   <  1:134031/139‑1 (MQ=255)
                                                                                     tCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTg                                                          <  1:222435/129‑1 (MQ=255)
                                                                                     tCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTg                                                          >  2:222435/1‑129 (MQ=255)
                                                                                       tGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGaa                                                                                                               <  2:55211/74‑1 (MQ=255)
                                                                                       tGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGaa                                                                                                               >  1:55211/1‑74 (MQ=255)
                                                                                       tGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAgaga                                              >  2:390968/1‑139 (MQ=255)
                                                                                        gCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCgg                                                       <  2:583098/129‑1 (MQ=255)
                                                                                        gCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCgg                                                       >  1:583098/1‑129 (MQ=255)
                                                                                         cTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGAtt                                            >  2:264270/1‑139 (MQ=255)
                                                                                         cTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGAtt                                            >  2:673175/1‑139 (MQ=255)
                                                                                               gTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGaaa                                                                                                              >  2:253280/1‑67 (MQ=255)
                                                                                               gTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGaaa                                                                                                              <  1:253280/67‑1 (MQ=255)
                                                                                               gTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCg                                      >  2:402680/1‑139 (MQ=255)
                                                                                               gTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCg                                      <  2:805911/139‑1 (MQ=255)
                                                                                               gTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCg                                      >  2:249825/1‑139 (MQ=255)
                                                                                                  gAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGt                                   <  1:91257/139‑1 (MQ=255)
                                                                                                  gAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGt                                   <  2:753215/139‑1 (MQ=255)
                                                                                                     tGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTcac                                <  1:249825/139‑1 (MQ=255)
                                                                                                        tGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCa                                                                                       <  1:795520/81‑1 (MQ=255)
                                                                                                        tGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCa                                                                                       >  2:795520/1‑81 (MQ=255)
                                                                                                          gCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                         >  2:394551/1‑61 (MQ=255)
                                                                                                          gCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                         <  1:394551/61‑1 (MQ=255)
                                                                                                          gCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTAATGAAAGTGCtgtg                                                                                                      >  2:924594/1‑64 (MQ=255)
                                                                                                          gCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTAATGAAAGTGCtgtg                                                                                                      <  1:924594/64‑1 (MQ=255)
                                                                                                                tCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATAc                     >  1:17417/1‑139 (MQ=255)
                                                                                                                  gTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCg                   <  2:267962/139‑1 (MQ=255)
                                                                                                                    ctcACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                         <  2:417882/51‑1 (MQ=255)
                                                                                                                    ctcACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                         >  1:417882/1‑51 (MQ=255)
                                                                                                                    ctcACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGaa                 <  1:390968/139‑1 (MQ=255)
                                                                                                                        cTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCa             <  1:789642/139‑1 (MQ=255)
                                                                                                                           tgtgGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCAAAg          >  1:391260/1‑139 (MQ=255)
                                                                                                                            gtgGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                         >  1:360304/1‑43 (MQ=255)
                                                                                                                            gtgGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                         <  2:360304/43‑1 (MQ=255)
                                                                                                                             tgGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCAAAGGt        <  2:17417/139‑1 (MQ=255)
                                                                                                                                   aaaTGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCg                   <  2:800622/122‑1 (MQ=255)
                                                                                                                                   aaaTGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCg                   >  1:800622/1‑122 (MQ=255)
                                                                                                                                   aaaTGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCAAAGGTATCGcc  >  1:980855/1‑139 (MQ=255)
                                                                                                                                     aTGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTA‑TTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGaa                 >  1:836648/1‑121 (MQ=255)
                                                                                                                                        |                                                                                                                                     
GGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGGGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCAAAGGTATCGCC  >  NC_000913/3732183‑3732452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A