Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,732,319	G→A	G40E (GGG→GAG)	xylG →	D‑xylose ABC transporter dual domain ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,732,319	0	G	A	100.0%	176.5 / NA	56	G40E (GGG→GAG)	xylG	D‑xylose ABC transporter dual domain ATPase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (29/27); total (29/27)

TAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGGGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCAAAGGTATCGCCAT  >  NC_000913/3732187‑3732454

tAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTgg                                                                                                                                   >  1:207208/1‑139 (MQ=255)

       aGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCa                                                                                                                            >  2:744442/1‑139 (MQ=255)

       aGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCa                                                                                                                            >  1:326313/1‑139 (MQ=255)

            ttATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCt                                                                                                                       <  2:207208/139‑1 (MQ=255)

                   ttATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGaaa                                                                                                                <  2:343006/139‑1 (MQ=255)

                     aTCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGt                                                                                                                                       >  2:456791/1‑114 (MQ=255)

                     aTCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGaaa                                                                                                                >  1:450355/1‑137 (MQ=255)

                     aTCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGt                                                                                                              >  1:101138/1‑139 (MQ=255)

                     aTCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCCGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGaaa                                                                                                                <  2:450355/137‑1 (MQ=255)

                        tACTTGGAATGAAGAACATTACCAGATCCTTCGGCAGTGTGAAGGCGATTGATAACGGCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAGCGCTGATGAAAGTGCt                                                                                                           <  2:405074/139‑1 (MQ=255)

                        tACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                           <  1:600733/139‑1 (MQ=255)

                           ttGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtg                                                                                                        >  2:227666/1‑139 (MQ=255)

                           ttGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtg                                                                                                        <  2:584349/139‑1 (MQ=255)

                           ttGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCtgtg                                                                                                        <  1:744442/139‑1 (MQ=255)

                              aaaTGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTgg                                                                                                     <  2:101138/139‑1 (MQ=255)

                                aTGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTa                                                                                                   >  2:549772/1‑139 (MQ=255)

                                    agaaCATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGtgttgt                                                                                                    >  2:181246/1‑134 (MQ=255)

                                    agaaCATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGt                                                                                                    <  1:181246/134‑1 (MQ=255)

                                    agaaCATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTa                                                                                               >  2:590517/1‑139 (MQ=255)

                                    agaaCATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTa                                                                                               >  1:653783/1‑139 (MQ=255)

                                         aTTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATcccc                                                                                          <  2:384423/139‑1 (MQ=255)

                                          ttACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCa                                                                                         <  2:636910/139‑1 (MQ=255)

                                           tACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCAt                                                                                        <  1:227666/139‑1 (MQ=255)

                                           tACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCAt                                                                                        <  1:549772/139‑1 (MQ=255)

                                             ccAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATgg                                                                                      >  1:546302/1‑139 (MQ=255)

                                                  aCCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCt                                                                                 >  2:665113/1‑139 (MQ=255)

                                                      tCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                           >  1:699121/1‑109 (MQ=255)

                                                      tCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                           <  2:699121/109‑1 (MQ=255)

                                                       cGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                             <  1:751863/106‑1 (MQ=255)

                                                       cGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                             >  2:751863/1‑106 (MQ=255)

                                                             tgtgAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCAGGGCTCCTCCGAAGGCGaa                                                                      >  1:123172/1‑139 (MQ=255)

                                                                  aGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCAt                                                                                        <  2:77616/116‑1 (MQ=255)

                                                                  aGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCAt                                                                                        >  1:77616/1‑116 (MQ=255)

                                                                       aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCa                                                                                                                            <  2:23438/75‑1 (MQ=255)

                                                                       aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCa                                                                                                                            >  1:23438/1‑75 (MQ=255)

                                                                       aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                             >  2:508532/1‑90 (MQ=255)

                                                                       aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTg                                                                                                             <  1:508532/90‑1 (MQ=255)

                                                                       aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTAt                                                                                                  >  1:579679/1‑101 (MQ=255)

                                                                       aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTAt                                                                                                  <  2:579679/101‑1 (MQ=255)

                                                                       aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTg                                                            >  2:530359/1‑139 (MQ=255)

                                                                       aTTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTg                                                            >  1:459747/1‑139 (MQ=255)

                                                                        ttGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGGGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGc                                                           <  2:123172/139‑1 (MQ=255)

                                                                         tGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCg                                                          >  2:374009/1‑139 (MQ=255)

                                                                              aCGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCtga                                                                                                                     >  2:770361/1‑75 (MQ=255)

                                                                              aCGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCtga                                                                                                                     <  1:770361/75‑1 (MQ=255)

                                                                                                 tGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTcac                                  <  2:358848/139‑1 (MQ=255)

                                                                                                    tGGCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATc                               >  1:142062/1‑139 (MQ=255)

                                                                                                      gCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                           <  1:571327/61‑1 (MQ=255)

                                                                                                      gCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCt                                                                                                           >  2:571327/1‑61 (MQ=255)

                                                                                                      gCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCcg                             >  2:624511/1‑139 (MQ=255)

                                                                                                      gCGAAATCGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCcg                             <  1:624511/139‑1 (MQ=255)

                                                                                                             cGTCTCACTTTGTGGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATAcc                      <  2:653783/139‑1 (MQ=255)

                                                                                                                        gtgGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCAAAgg           >  2:426732/1‑139 (MQ=255)

                                                                                                                         tgGGGAAAATGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCAAAGGt          <  1:426732/139‑1 (MQ=255)

                                                                                                                              aaaaTGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCAAAGGTATCGc     <  1:116647/139‑1 (MQ=255)

                                                                                                                                 aTGAGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCAAAGGTATCGCcat  >  1:160020/1‑139 (MQ=255)

TAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCGAAATCGTCTCACTTTGTGGGGAAAATGGGTCTGGTAAATCAACGCTGATGAAAGTGCTGTGTGGTATTTATCCCCATGGCTCCTACGAAGGCGAAATTATTTTTGCGGGAGAAGAGATTCAGGCGAGTCACATCCGCGATACCGAACGCAAAGGTATCGCCAT  >  NC_000913/3732187‑3732454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A