Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I209 R1
|
216 |
14.4 |
815930 |
96.1% |
784108 |
85.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
579,943 |
(T)8→7 |
intergenic (+57/+193) |
appY → / ← ompT |
DNA‑binding transcriptional activator AppY/omptin family outer membrane protease OmpT |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 579,936 | 0 | T | . | 100.0%
| 10.5
/ NA
| 4 | intergenic (+50/+200) | appY/ompT | DNA‑binding transcriptional activator AppY/omptin family outer membrane protease OmpT |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (0/4); total (0/4) |
AAGTTGTCTACATGTTAATTATAATATTATACAGCGTTTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATAC > NZ_CP009273/579900‑580000
|
aaGTTGTCTACATGTTAATTATAATATTATACAGCG‑TTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGcc < 2:222911/90‑1 (MQ=255)
aaGTTGTCTACATGTTAATTATAATATTATACAGCG‑TTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGcc < 2:355944/90‑1 (MQ=255)
aaGTTGTCTACATGTTAATTATAATATTATACAGCG‑TTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGcc < 2:58774/90‑1 (MQ=255)
cATGTTAATTATAATATTATACAGCG‑TTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTatac < 2:133926/90‑1 (MQ=255)
|
AAGTTGTCTACATGTTAATTATAATATTATACAGCGTTTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATAC > NZ_CP009273/579900‑580000
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
