Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,231,349 A→C 91.7% Q227P (CAG→CCG)  ygjR → putative NAD(P)‑dependent dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,231,3490AC91.7% 949.9 / 39.8 252Q227P (CAG→CCG) ygjRputative NAD(P)‑dependent dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (13/8);  new base C (108/123);  total (121/131)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 252 total aligned reads displayed.

GATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCAT  >  CP009273/3231204‑3231494
                                                                                                                                                 |                                                                                                                                                 
gATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCAc                                                                                                                                               >  2:4125745/1‑150 (MQ=255)
      ggTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCaaaa                                                                                                                                         >  2:1393001/1‑150 (MQ=255)
       gTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAg                                                                                                                                        <  1:2210524/150‑1 (MQ=255)
            tACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGt                                                                                                                                   >  2:3717943/1‑150 (MQ=255)
            tACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGt                                                                                                                                   <  2:1802070/150‑1 (MQ=255)
            tACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGt                                                                                                                                   >  2:4605289/1‑150 (MQ=255)
            tACTGTCTGGCGTCGGCGGTGGCGTTATTTGGCGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGt                                                                                                                                   <  2:2118690/150‑1 (MQ=255)
             aCTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTg                                                                                                                                  >  1:3360395/1‑150 (MQ=255)
               tGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGAt                                                                                                                                <  2:3482877/150‑1 (MQ=255)
                gTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGAtt                                                                                                                               <  1:1308192/150‑1 (MQ=255)
                gTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGAtt                                                                                                                               <  1:3856312/150‑1 (MQ=255)
                  cTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCt                                                                                                                             <  2:1752623/150‑1 (MQ=255)
                  cTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCt                                                                                                                             <  2:3055106/150‑1 (MQ=255)
                   tGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTg                                                                                                                            >  2:1951374/1‑150 (MQ=255)
                    ggCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGt                                                                                                                           <  2:1330458/150‑1 (MQ=255)
                     gCGTCGGCGGTGGCGTTATTTGGTGAGCCGATAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTc                                                                                                                          <  1:929744/150‑1 (MQ=255)
                       gTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCt                                                                                                                        <  2:1002897/150‑1 (MQ=255)
                         cggcggTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTgg                                                                                                                      <  2:1766788/150‑1 (MQ=255)
                         cggcggTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTgg                                                                                                                      >  1:4048540/1‑150 (MQ=255)
                          ggcggTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGgc                                                                                                                     <  1:639543/150‑1 (MQ=255)
                            cggTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGgcga                                                                                                                   >  1:1013677/1‑150 (MQ=255)
                             ggTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGgcgag                                                                                                                  >  2:567255/1‑150 (MQ=255)
                               tGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGgcgagcg                                                                                                                >  1:2051979/1‑150 (MQ=255)
                                ggCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGgcgagcga                                                                                                               >  1:1744243/1‑150 (MQ=255)
                                  cGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCgaga                                                                                                             >  2:617354/1‑150 (MQ=255)
                                    ttATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGAtt                                                                                                           >  2:3900510/1‑150 (MQ=255)
                                     tATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTc                                                                                                          <  2:4101837/150‑1 (MQ=255)
                                       tttGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAg                                                                                                        >  1:3975905/1‑150 (MQ=255)
                                       tttGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAg                                                                                                        >  1:4343698/1‑150 (MQ=255)
                                        ttGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAgg                                                                                                       >  1:3616155/1‑150 (MQ=255)
                                          ggTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGc                                                                                                     >  1:379056/1‑150 (MQ=255)
                                            tGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGa                                                                                                   >  2:1670575/1‑150 (MQ=255)
                                             gAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGaa                                                                                                  >  1:709594/1‑150 (MQ=255)
                                              aGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAg                                                                                                 <  1:3384647/150‑1 (MQ=255)
                                               gCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGc                                                                                                <  1:2148319/150‑1 (MQ=255)
                                                  gAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAgg                                                                                             >  2:4213316/1‑150 (MQ=255)
                                                  gAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAgg                                                                                             <  2:363675/150‑1 (MQ=255)
                                                  gAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAgg                                                                                             >  2:1331901/1‑150 (MQ=255)
                                                   aaaaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGa                                                                                            >  2:2526602/1‑150 (MQ=255)
                                                   aaaaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGa                                                                                            >  2:149260/1‑150 (MQ=255)
                                                   aaaaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGa                                                                                            >  2:1971934/1‑150 (MQ=255)
                                                    aaaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGAt                                                                                           <  2:2404781/150‑1 (MQ=255)
                                                      aGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCg                                                                                         >  1:2140910/1‑150 (MQ=255)
                                                       gCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGc                                                                                        <  1:39765/150‑1 (MQ=255)
                                                        cGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCt                                                                                       <  2:2432882/150‑1 (MQ=255)
                                                         gTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTg                                                                                      >  2:4327368/1‑150 (MQ=255)
                                                         gTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTg                                                                                      <  1:2023668/150‑1 (MQ=255)
                                                         gTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTg                                                                                      <  1:3090619/150‑1 (MQ=255)
                                                         gTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTg                                                                                      <  1:3090736/150‑1 (MQ=255)
                                                          tGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTgg                                                                                     <  1:2355333/150‑1 (MQ=255)
                                                           gCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGt                                                                                    >  2:3670220/1‑150 (MQ=255)
                                                             aGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGa                                                                                  <  1:3476150/150‑1 (MQ=255)
                                                              ggCAACCGCCAGTTTGTTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAt                                                                                 <  1:184916/150‑1 (MQ=255)
                                                              ggCAACCGCCAGTTTGTTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAt                                                                                 <  1:134419/150‑1 (MQ=255)
                                                              ggCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAt                                                                                 <  1:1903269/150‑1 (MQ=255)
                                                               gCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTTCGGTGATTTCAGCGTCACCTTGCCTCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGCCGAAGCAGGATCGCTGGTGAtt                                                                                <  2:69946/150‑1 (MQ=255)
                                                               gCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAtt                                                                                <  2:822591/150‑1 (MQ=255)
                                                                   ccGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGaaa                                                                            <  1:1539766/150‑1 (MQ=255)
                                                                      ccAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCCGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAAc                                                                         >  2:333538/1‑150 (MQ=255)
                                                                       cAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACt                                                                        <  2:370870/150‑1 (MQ=255)
                                                                         gTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGt                                                                      >  2:1199166/1‑150 (MQ=255)
                                                                         gTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGt                                                                      >  2:1039599/1‑150 (MQ=255)
                                                                             gCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTTGTTATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGa                                                                  <  1:189491/150‑1 (MQ=255)
                                                                                ggCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATg                                                               <  2:1853436/150‑1 (MQ=255)
                                                                                  cAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGcc                                                             >  2:3601231/1‑150 (MQ=255)
                                                                                    aGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTTATTTCAGCGTCACCATGCCGCACTCAAAAGTCAGTGATTCAGTCCAGGCGAGCGAGAATCAGGGCGAAGCATGAACGCTGGTGATTGAAAAACTGTCTGAAATCCAg                                                           >  1:4061783/1‑150 (MQ=255)
                                                                                    aGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAg                                                           <  2:2597482/150‑1 (MQ=255)
                                                                                     gcggcgTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGa                                                          <  1:2808771/150‑1 (MQ=255)
                                                                                        gcgTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAg                                                       <  1:2517777/150‑1 (MQ=255)
                                                                                          gTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAgtg                                                     <  2:4367635/150‑1 (MQ=255)
                                                                                           ttGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGTGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAgtgt                                                    <  1:3211787/150‑1 (MQ=255)
                                                                                             gACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGc                                                  >  2:4498727/1‑150 (MQ=255)
                                                                                             gACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGc                                                  >  2:3127989/1‑150 (MQ=255)
                                                                                              aCGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCt                                                 <  1:1820155/150‑1 (MQ=255)
                                                                                              aCGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCt                                                 <  1:654729/150‑1 (MQ=255)
                                                                                                gCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTc                                               <  2:891993/150‑1 (MQ=255)
                                                                                                     gggCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGcc                                          <  2:1429045/150‑1 (MQ=255)
                                                                                                      ggCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCcg                                         >  1:2061026/1‑150 (MQ=255)
                                                                                                         gtggtggtgATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGt                                      >  1:1611811/1‑150 (MQ=255)
                                                                                                          tggtggtgATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTg                                     <  1:2165681/150‑1 (MQ=255)
                                                                                                          tggtggtgATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTg                                     <  1:4481036/150‑1 (MQ=255)
                                                                                                             tggtgATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCa                                  >  1:4603811/1‑150 (MQ=255)
                                                                                                              ggtgATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAGTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAg                                 >  1:278185/1‑150 (MQ=255)
                                                                                                               gtgATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGc                                <  1:2512234/150‑1 (MQ=255)
                                                                                                               gtgATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGc                                <  1:791789/150‑1 (MQ=255)
                                                                                                                 gATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCa                              >  1:4302616/1‑150 (MQ=255)
                                                                                                                  aTGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCaa                             >  2:2904963/1‑150 (MQ=255)
                                                                                                                    ggATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAAt                           <  2:4048530/150‑1 (MQ=255)
                                                                                                                      aTTACTGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGAGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGAGGCAGCCAAATGc                         >  2:3133634/1‑150 (MQ=255)
                                                                                                                           ggTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGAt                    >  2:3457163/1‑150 (MQ=255)
                                                                                                                                 ttCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCAcc              <  2:2755490/150‑1 (MQ=255)
                                                                                                                                 ttCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCAcc              >  2:3916565/1‑150 (MQ=255)
                                                                                                                                   cAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCa            <  2:4303260/150‑1 (MQ=255)
                                                                                                                                      cGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGcc         <  2:3112639/150‑1 (MQ=255)
                                                                                                                                      cGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGcc         >  2:1626725/1‑150 (MQ=255)
                                                                                                                                      cGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGcc         <  2:2389774/150‑1 (MQ=255)
                                                                                                                                       gTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCg        <  2:275/150‑1 (MQ=255)
                                                                                                                                         cACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgca      <  2:207130/150‑1 (MQ=255)
                                                                                                                                         cACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgca      >  2:638339/1‑150 (MQ=255)
                                                                                                                                             ttGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCat  <  2:3842452/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCAT  >  CP009273/3231204‑3231494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATAT  >  CP009273/3231200‑3231496
                                                                                                                                                     |                                                                                                                                                   
TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCC                                                                                                                                                     >  SRR19973072.492106/1‑150 (MQ=60)
 ATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCG                                                                                                                                                    >  SRR19973072.3119204/1‑150 (MQ=60)
    GATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCAC                                                                                                                                                 <  SRR19973072.182828/150‑1 (MQ=60)
           GTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAG                                                                                                                                          <  SRR19973072.2215327/150‑1 (MQ=60)
            TTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGT                                                                                                                                         >  SRR19973072.20596/1‑150 (MQ=60)
                TACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGT                                                                                                                                     >  SRR19973072.665401/1‑150 (MQ=60)
                 ACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTG                                                                                                                                    >  SRR19973072.3367521/1‑150 (MQ=60)
                    GTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATT                                                                                                                                 <  SRR19973072.1311037/150‑1 (MQ=60)
                    GTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATT                                                                                                                                 <  SRR19973072.795287/150‑1 (MQ=60)
                    GTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATT                                                                                                                                 <  SRR19973072.3864345/150‑1 (MQ=60)
                      CTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCT                                                                                                                               >  SRR19973072.659569/1‑150 (MQ=60)
                      CTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCT                                                                                                                               >  SRR19973072.4068161/1‑150 (MQ=60)
                        GGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGT                                                                                                                             <  SRR19973072.2046933/150‑1 (MQ=60)
                         GCGTCGGCGGTGGCGTTATTTGGTGAGCCGATAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTC                                                                                                                            <  SRR19973072.931772/150‑1 (MQ=60)
                             CGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGG                                                                                                                        >  SRR19973072.4057030/1‑150 (MQ=60)
                             CGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGG                                                                                                                        >  SRR19973072.4057040/1‑150 (MQ=60)
                              GGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGC                                                                                                                       >  SRR19973072.3373482/1‑150 (MQ=60)
                              GGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGC                                                                                                                       <  SRR19973072.641000/150‑1 (MQ=60)
                                CGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGA                                                                                                                     >  SRR19973072.1015884/1‑150 (MQ=60)
                                  GTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGC                                                                                                                   >  SRR19973072.2148169/1‑150 (MQ=60)
                                  GTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGC                                                                                                                   >  SRR19973072.4004028/1‑150 (MQ=60)
                                   TGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCG                                                                                                                  >  SRR19973072.2056446/1‑150 (MQ=60)
                                    GGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGA                                                                                                                 >  SRR19973072.1748055/1‑150 (MQ=60)
                                     GCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAG                                                                                                                <  SRR19973072.4352457/150‑1 (MQ=60)
                                      CGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAACGAGA                                                                                                               <  SRR19973072.2194809/150‑1 (MQ=60)
                                           TTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAG                                                                                                          >  SRR19973072.4352794/1‑150 (MQ=60)
                                           TTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAG                                                                                                          >  SRR19973072.3984226/1‑150 (MQ=60)
                                           TTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAG                                                                                                          >  SRR19973072.3984320/1‑150 (MQ=60)
                                            TTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGG                                                                                                         >  SRR19973072.3855522/1‑150 (MQ=60)
                                            TTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGG                                                                                                         >  SRR19973072.3623769/1‑150 (MQ=60)
                                              GGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGC                                                                                                       >  SRR19973072.379877/1‑150 (MQ=60)
                                                TGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGA                                                                                                     >  SRR19973072.4305125/1‑150 (MQ=60)
                                                 GAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAA                                                                                                    >  SRR19973072.711138/1‑150 (MQ=60)
                                                 GAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAA                                                                                                    >  SRR19973072.711154/1‑150 (MQ=60)
                                                 GAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAA                                                                                                    <  SRR19973072.3160833/150‑1 (MQ=60)
                                                  AGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAG                                                                                                   <  SRR19973072.3391801/150‑1 (MQ=60)
                                                  AGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAG                                                                                                   >  SRR19973072.1618157/1‑150 (MQ=60)
                                                   GCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGC                                                                                                  <  SRR19973072.2152995/150‑1 (MQ=60)
                                                     CGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAG                                                                                                <  SRR19973072.496436/150‑1 (MQ=60)
                                                      GAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGG                                                                                               <  SRR19973072.97229/150‑1 (MQ=60)
                                                      GAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGG                                                                                               <  SRR19973072.2415290/150‑1 (MQ=60)
                                                       AAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGA                                                                                              >  SRR19973072.1123964/1‑150 (MQ=60)
                                                        AAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGAT                                                                                             >  SRR19973072.1995303/1‑150 (MQ=60)
                                                          AGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCG                                                                                           >  SRR19973072.2145574/1‑150 (MQ=60)
                                                          AGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCG                                                                                           >  SRR19973072.2145605/1‑150 (MQ=60)
                                                           GCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGC                                                                                          <  SRR19973072.39875/150‑1 (MQ=60)
                                                            CGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCT                                                                                         <  SRR19973072.1814163/150‑1 (MQ=60)
                                                            CGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCT                                                                                         <  SRR19973072.1814243/150‑1 (MQ=60)
                                                             GTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTG                                                                                        <  SRR19973072.2028044/150‑1 (MQ=60)
                                                             GTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTG                                                                                        <  SRR19973072.3097250/150‑1 (MQ=60)
                                                             GTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTG                                                                                        <  SRR19973072.3097156/150‑1 (MQ=60)
                                                             GTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTG                                                                                        <  SRR19973072.3097133/150‑1 (MQ=60)
                                                              TGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGG                                                                                       <  SRR19973072.2360399/150‑1 (MQ=60)
                                                                 AGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGA                                                                                    <  SRR19973072.3483414/150‑1 (MQ=60)
                                                                 AGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGA                                                                                    <  SRR19973072.2542335/150‑1 (MQ=60)
                                                                  GGCAACCGCCAGTTTGTTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAT                                                                                   <  SRR19973072.185381/150‑1 (MQ=60)
                                                                  GGCAACCGCCAGTTTGTTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAT                                                                                   <  SRR19973072.185646/150‑1 (MQ=60)
                                                                  GGCAACCGCCAGTTTGTTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAT                                                                                   <  SRR19973072.134687/150‑1 (MQ=60)
                                                                  GGCAACCGCCAGTTTGTTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAT                                                                                   <  SRR19973072.134761/150‑1 (MQ=60)
                                                                  GGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAT                                                                                   <  SRR19973072.1907330/150‑1 (MQ=60)
                                                                  GGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAT                                                                                   <  SRR19973072.2798265/150‑1 (MQ=60)
                                                                  GGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAT                                                                                   >  SRR19973072.2597836/1‑150 (MQ=60)
                                                                  GGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAT                                                                                   >  SRR19973072.1547869/1‑150 (MQ=60)
                                                                  GGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAT                                                                                   <  SRR19973072.1907329/150‑1 (MQ=60)
                                                                       CCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAA                                                                              <  SRR19973072.1543022/150‑1 (MQ=60)
                                                                        CGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGTGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAA                                                                             <  SRR19973072.3484161/150‑1 (MQ=60)
                                                                           CAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACT                                                                          <  SRR19973072.2169046/150‑1 (MQ=60)
                                                                            AGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTTGTGATGGATTAAGGTGATTTCAGCGTAACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGAAGGATCGCTTGTGATTGAAAAACTG                                                                         >  SRR19973072.29948/1‑150 (MQ=60)
                                                                            AGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTG                                                                         >  SRR19973072.4468590/1‑150 (MQ=60)
                                                                            AGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTG                                                                         >  SRR19973072.3412184/1‑150 (MQ=60)
                                                                            AGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTG                                                                         >  SRR19973072.2073358/1‑150 (MQ=60)
                                                                                 GCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTTGTTATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGA                                                                    <  SRR19973072.189972/150‑1 (MQ=60)
                                                                                 GCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGA                                                                    <  SRR19973072.189937/150‑1 (MQ=60)
                                                                                   TGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAAT                                                                  >  SRR19973072.3216396/1‑150 (MQ=60)
                                                                                    GGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATG                                                                 <  SRR19973072.2887196/150‑1 (MQ=60)
                                                                                     GCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGC                                                                >  SRR19973072.1344813/1‑150 (MQ=60)
                                                                                        AGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTTATTTCAGCGTCACCATGCCGCACTCAAAAGTCAGTGATTCAGTCCAGGCGAGCGAGAATCAGGGCGAAGCATGAACGCTGGTGATTGAAAAACTGTCTGAAATCCAG                                                             >  SRR19973072.4070302/1‑150 (MQ=60)
                                                                                        AGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAG                                                             <  SRR19973072.4399686/150‑1 (MQ=60)
                                                                                         GCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGA                                                            <  SRR19973072.2814786/150‑1 (MQ=60)
                                                                                            GCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAG                                                         <  SRR19973072.2523249/150‑1 (MQ=60)
                                                                                            GCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAG                                                         <  SRR19973072.2523250/150‑1 (MQ=60)
                                                                                               TTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGTGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGT                                                      <  SRR19973072.3218580/150‑1 (MQ=60)
                                                                                                TGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGCGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTG                                                     >  SRR19973072.2390210/1‑150 (MQ=60)
                                                                                                 GACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGC                                                    <  SRR19973072.861035/150‑1 (MQ=60)
                                                                                                 GACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGC                                                    >  SRR19973072.3946076/1‑150 (MQ=60)
                                                                                                 GACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGC                                                    >  SRR19973072.3946087/1‑150 (MQ=60)
                                                                                                  ACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCT                                                   <  SRR19973072.656210/150‑1 (MQ=60)
                                                                                                  ACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCT                                                   <  SRR19973072.655642/150‑1 (MQ=60)
                                                                                                  ACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCT                                                   <  SRR19973072.1824121/150‑1 (MQ=60)
                                                                                                  ACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCT                                                   >  SRR19973072.3258886/1‑150 (MQ=60)
                                                                                                        AGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGC                                             >  SRR19973072.3704715/1‑150 (MQ=60)
                                                                                                        AGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGC                                             >  SRR19973072.1573122/1‑150 (MQ=60)
                                                                                                          GGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCG                                           >  SRR19973072.2065512/1‑150 (MQ=60)
                                                                                                             GTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGT                                        >  SRR19973072.459383/1‑150 (MQ=60)
                                                                                                             GTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGT                                        >  SRR19973072.1615306/1‑150 (MQ=60)
                                                                                                              TGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTG                                       <  SRR19973072.4490496/150‑1 (MQ=60)
                                                                                                              TGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTG                                       <  SRR19973072.2170646/150‑1 (MQ=60)
                                                                                                              TGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTG                                       <  SRR19973072.2170401/150‑1 (MQ=60)
                                                                                                                 TGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCA                                    >  SRR19973072.4613474/1‑150 (MQ=60)
                                                                                                                 TGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCA                                    <  SRR19973072.2328925/150‑1 (MQ=60)
                                                                                                                  GGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAGTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAG                                   >  SRR19973072.278833/1‑150 (MQ=60)
                                                                                                                   GTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGC                                  <  SRR19973072.2517694/150‑1 (MQ=60)
                                                                                                                   GTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGC                                  <  SRR19973072.793478/150‑1 (MQ=60)
                                                                                                                     GATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCA                                >  SRR19973072.4311598/1‑150 (MQ=60)
                                                                                                                      ATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAA                               <  SRR19973072.568583/150‑1 (MQ=60)
                                                                                                                              CGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGA                       <  SRR19973072.2059678/150‑1 (MQ=60)
                                                                                                                                 TGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCT                    <  SRR19973072.3438667/150‑1 (MQ=60)
                                                                                                                                            TCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGC         >  SRR19973072.4093417/1‑150 (MQ=60)
                                                                                                                                             CACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGGGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCA        >  SRR19973072.2012304/1‑150 (MQ=60)
                                                                                                                                                 TTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGATCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCAT    <  SRR19973072.3724591/150‑1 (MQ=60)
                                                                                                                                                 TTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCAT    <  SRR19973072.1396034/150‑1 (MQ=60)
                                                                                                                                                 TTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCAT    >  SRR19973072.3334716/1‑150 (MQ=60)
                                                                                                                                                   GCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATAT  <  SRR19973072.1207773/150‑1 (MQ=60)
                                                                                                                                                   GCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATAT  <  SRR19973072.312793/150‑1 (MQ=60)
                                                                                                                                                   GCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATAT  <  SRR19973072.3544191/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATAT  >  CP009273/3231200‑3231496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑