Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,231,349 A→C 100% Q227P (CAG→CCG)  ygjR → putative NAD(P)‑dependent dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,231,3490AC96.2% 720.3 / 5.6 182Q227P (CAG→CCG) ygjRputative NAD(P)‑dependent dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (2/5);  new base C (80/95);  total (82/100)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 188 total aligned reads displayed.

GATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCA  >  CP009273/3231204‑3231493
                                                                                                                                                 |                                                                                                                                                
gATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTgccgc                                                                                                                                                >  2:1255446/1‑148 (MQ=255)
 atatCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTgccgca                                                                                                                                               >  1:3732933/1‑148 (MQ=255)
 atatCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTgccgca                                                                                                                                               <  1:3402686/148‑1 (MQ=255)
 atatCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTgccgca                                                                                                                                               <  1:3372058/148‑1 (MQ=255)
    tCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTTCTGGCAAGCGGCGGTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTc                                                                                                                                            >  1:1458941/1‑148 (MQ=255)
     cGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCa                                                                                                                                           >  2:3381029/1‑148 (MQ=255)
     cGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCa                                                                                                                                           >  2:1642353/1‑148 (MQ=255)
     cGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCa                                                                                                                                           >  2:130970/1‑148 (MQ=255)
       gTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCaaa                                                                                                                                         <  2:534895/148‑1 (MQ=255)
             aCTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAg                                                                                                                                   <  1:1322185/148‑1 (MQ=255)
               tGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTg                                                                                                                                 <  1:1369766/148‑1 (MQ=255)
               tGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTg                                                                                                                                 <  1:2031449/148‑1 (MQ=255)
                gTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGa                                                                                                                                <  2:2637197/148‑1 (MQ=255)
                  cTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGAtt                                                                                                                              >  1:2453247/1‑148 (MQ=255)
                  cTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGAtt                                                                                                                              <  2:1321799/148‑1 (MQ=255)
                   tGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTc                                                                                                                             >  1:615568/1‑148 (MQ=255)
                    ggCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACACCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCt                                                                                                                            <  1:402293/148‑1 (MQ=255)
                     gCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTg                                                                                                                           <  2:372112/148‑1 (MQ=255)
                     gCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTg                                                                                                                           >  1:1516365/1‑148 (MQ=255)
                     gCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTg                                                                                                                           <  2:3755462/148‑1 (MQ=255)
                      cGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGt                                                                                                                          <  1:845435/148‑1 (MQ=255)
                      cGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGt                                                                                                                          <  1:3272811/148‑1 (MQ=255)
                       gTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTc                                                                                                                         <  1:2824448/148‑1 (MQ=255)
                                ggCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGgcgagc                                                                                                                >  2:2771702/1‑148 (MQ=255)
                                ggCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGgcgagc                                                                                                                <  2:925569/148‑1 (MQ=255)
                                 gCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGgcgagcg                                                                                                               >  1:2832710/1‑148 (MQ=255)
                                    ttATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCgaga                                                                                                            >  2:1760691/1‑148 (MQ=255)
                                    ttATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCgaga                                                                                                            <  2:1899793/148‑1 (MQ=255)
                                    ttATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCgaga                                                                                                            <  1:3172814/148‑1 (MQ=255)
                                      aTTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGAtt                                                                                                          <  1:3689736/148‑1 (MQ=255)
                                      aTTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGAtt                                                                                                          <  1:3195232/148‑1 (MQ=255)
                                       tttGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTc                                                                                                         <  2:2748967/148‑1 (MQ=255)
                                        ttGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCa                                                                                                        >  2:2381619/1‑148 (MQ=255)
                                        ttGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCa                                                                                                        >  2:1340411/1‑148 (MQ=255)
                                        ttGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCa                                                                                                        >  2:2376024/1‑148 (MQ=255)
                                        ttGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCa                                                                                                        <  1:2388033/148‑1 (MQ=255)
                                          ggTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAgg                                                                                                      <  1:3814518/148‑1 (MQ=255)
                                          ggTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAgg                                                                                                      <  2:1895168/148‑1 (MQ=255)
                                           gTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAggg                                                                                                     <  2:1436954/148‑1 (MQ=255)
                                              aGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGa                                                                                                  <  1:1569124/148‑1 (MQ=255)
                                              aGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGa                                                                                                  >  1:2035135/1‑148 (MQ=255)
                                               gCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGaa                                                                                                 <  2:3054201/148‑1 (MQ=255)
                                                 cGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGc                                                                                               >  2:3735881/1‑148 (MQ=255)
                                                  gAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCa                                                                                              <  1:2014812/148‑1 (MQ=255)
                                                    aaaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAgg                                                                                            >  2:175326/1‑148 (MQ=255)
                                                     aaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGa                                                                                           >  1:3463811/1‑148 (MQ=255)
                                                     aaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGa                                                                                           <  2:3729679/148‑1 (MQ=255)
                                                      aGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGAt                                                                                          >  2:849667/1‑148 (MQ=255)
                                                       gCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATc                                                                                         >  1:304983/1‑148 (MQ=255)
                                                        cGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCg                                                                                        <  1:1462762/148‑1 (MQ=255)
                                                          tGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCt                                                                                      <  1:2558097/148‑1 (MQ=255)
                                                           gCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCCAAAGTCACTGATTCGGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTg                                                                                     >  2:2571418/1‑148 (MQ=255)
                                                           gCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTg                                                                                     <  2:1520209/148‑1 (MQ=255)
                                                                       cAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGaaaaa                                                                         <  2:2230001/148‑1 (MQ=255)
                                                                        aGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAAc                                                                        <  1:2274579/148‑1 (MQ=255)
                                                                        aGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAAc                                                                        <  2:1938541/148‑1 (MQ=255)
                                                                          tttGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGCTTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTg                                                                      >  2:1954241/1‑148 (MQ=255)
                                                                          tttGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTg                                                                      <  1:11409/148‑1 (MQ=255)
                                                                           ttGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGt                                                                     <  2:938641/148‑1 (MQ=255)
                                                                             gCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCt                                                                   >  2:2927837/1‑148 (MQ=255)
                                                                               tGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGa                                                                 <  1:392730/148‑1 (MQ=255)
                                                                                ggCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGaa                                                                <  1:1760691/148‑1 (MQ=255)
                                                                                  cAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATg                                                              >  2:1070991/1‑148 (MQ=255)
                                                                                   aaGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGc                                                             <  2:1076173/148‑1 (MQ=255)
                                                                                   aaGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGc                                                             <  2:2422754/148‑1 (MQ=255)
                                                                                    aGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGcc                                                            >  2:2197425/1‑148 (MQ=255)
                                                                                    aGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGcc                                                            >  2:2196540/1‑148 (MQ=255)
                                                                                     gcggcgTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGTCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCa                                                           >  1:1081408/1‑148 (MQ=255)
                                                                                          gTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAg                                                      <  1:2130967/148‑1 (MQ=255)
                                                                                            tGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAgtg                                                    >  1:3435942/1‑148 (MQ=255)
                                                                                            tGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAgtg                                                    <  2:3767132/148‑1 (MQ=255)
                                                                                            tGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAgtg                                                    <  2:2757382/148‑1 (MQ=255)
                                                                                              aCGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGtgtg                                                  >  1:2788202/1‑148 (MQ=255)
                                                                                               cGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGc                                                 <  1:3502703/148‑1 (MQ=255)
                                                                                                 cccAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCtt                                               <  2:1764203/148‑1 (MQ=255)
                                                                                                     gggCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGATTCGTg                                           >  2:3671542/1‑148 (MQ=255)
                                                                                                        cGTGGTGGTGATGGATTACAGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCcg                                        <  1:1867333/148‑1 (MQ=255)
                                                                                                              ggtgATGGATTACGGTGATTTCAGCGTTACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGc                                  >  1:1098934/1‑148 (MQ=255)
                                                                                                                    ggATTACGGTGCTTTCAGCTTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGATTGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCaa                            <  2:3454222/148‑1 (MQ=255)
                                                                                                                    ggATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCaa                            <  1:1573498/148‑1 (MQ=255)
                                                                                                                    ggATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCaa                            <  1:2661876/148‑1 (MQ=255)
                                                                                                                     gATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCaaa                           <  1:907958/148‑1 (MQ=255)
                                                                                                                       ttACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATg                         >  2:1819524/1‑148 (MQ=255)
                                                                                                                           ggTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAgg                     >  1:717069/1‑148 (MQ=255)
                                                                                                                             tGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGAt                   <  1:1951205/148‑1 (MQ=255)
                                                                                                                                 ttCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCa               <  1:113319/148‑1 (MQ=255)
                                                                                                                                   cAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCAcc             <  1:2768972/148‑1 (MQ=255)
                                                                                                                                   cAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCAcc             <  1:1687921/148‑1 (MQ=255)
                                                                                                                                     gCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCa           >  2:264079/1‑148 (MQ=255)
                                                                                                                                     gCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCa           >  1:3532629/1‑148 (MQ=255)
                                                                                                                                         cACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCg       >  1:267164/1‑148 (MQ=255)
                                                                                                                                         cACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCg       >  1:2009797/1‑148 (MQ=255)
                                                                                                                                         cACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCg       >  1:2009692/1‑148 (MQ=255)
                                                                                                                                         cACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCg       >  1:264848/1‑148 (MQ=255)
                                                                                                                                          aCCTTGCCGCACACAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgc      >  2:1502487/1‑148 (MQ=255)
                                                                                                                                           ccTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgca     >  1:2970866/1‑148 (MQ=255)
                                                                                                                                           ccTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgca     >  1:2970861/1‑148 (MQ=255)
                                                                                                                                           ccTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAATCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgca     >  1:3633299/1‑148 (MQ=255)
                                                                                                                                            cTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgcag    >  1:1261539/1‑148 (MQ=255)
                                                                                                                                              tGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgcagca  <  1:1019901/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCA  >  CP009273/3231204‑3231493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATAT  >  CP009273/3231200‑3231496
                                                                                                                                                     |                                                                                                                                                   
TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCC                                                                                                                                                     <  SRR19973069.996396/150‑1 (MQ=60)
TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCC                                                                                                                                                     <  SRR19973069.2927052/150‑1 (MQ=60)
TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCC                                                                                                                                                     >  SRR19973069.2531329/1‑150 (MQ=60)
 ATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCG                                                                                                                                                    >  SRR19973069.715611/1‑150 (MQ=60)
 ATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCG                                                                                                                                                    >  SRR19973069.1446980/1‑150 (MQ=60)
   GGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCA                                                                                                                                                  >  SRR19973069.3757271/1‑150 (MQ=60)
     ATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACT                                                                                                                                                <  SRR19973069.3424882/150‑1 (MQ=60)
     ATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACT                                                                                                                                                <  SRR19973069.3394104/150‑1 (MQ=60)
     ATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACT                                                                                                                                                <  SRR19973069.3394097/150‑1 (MQ=60)
     ATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACT                                                                                                                                                <  SRR19973069.1512511/150‑1 (MQ=60)
      TATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTTCTGGCAAGCGGCGGTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTC                                                                                                                                               >  SRR19973069.1469119/1‑150 (MQ=60)
            TTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGT                                                                                                                                         <  SRR19973069.3830503/150‑1 (MQ=60)
              TTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCA                                                                                                                                       <  SRR19973069.1103455/150‑1 (MQ=60)
                 ACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTG                                                                                                                                    <  SRR19973069.1331270/150‑1 (MQ=60)
                   TGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGAT                                                                                                                                  <  SRR19973069.1379296/150‑1 (MQ=60)
                   TGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGAT                                                                                                                                  <  SRR19973069.2045560/150‑1 (MQ=60)
                   TGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGAT                                                                                                                                  <  SRR19973069.1379281/150‑1 (MQ=60)
                    GTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATT                                                                                                                                 >  SRR19973069.2469744/1‑150 (MQ=60)
                     TCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTC                                                                                                                                >  SRR19973069.619616/1‑150 (MQ=60)
                     TCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTC                                                                                                                                >  SRR19973069.619675/1‑150 (MQ=60)
                       TGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTG                                                                                                                              >  SRR19973069.1526832/1‑150 (MQ=60)
                        GGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACACCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGT                                                                                                                             <  SRR19973069.405243/150‑1 (MQ=60)
                        GGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACACCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGT                                                                                                                             <  SRR19973069.386799/150‑1 (MQ=60)
                          CGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCC                                                                                                                           <  SRR19973069.3294240/150‑1 (MQ=60)
                          CGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCC                                                                                                                           <  SRR19973069.1142046/150‑1 (MQ=60)
                          CGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCC                                                                                                                           <  SRR19973069.851365/150‑1 (MQ=60)
                           GTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCT                                                                                                                          <  SRR19973069.2843125/150‑1 (MQ=60)
                           GTCGGCGGTGGAGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCT                                                                                                                          <  SRR19973069.2843127/150‑1 (MQ=60)
                            TCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTG                                                                                                                         <  SRR19973069.3530196/150‑1 (MQ=60)
                                 GGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAG                                                                                                                    <  SRR19973069.2150825/150‑1 (MQ=60)
                                   TGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCG                                                                                                                  >  SRR19973069.2851418/1‑150 (MQ=60)
                                        TTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATT                                                                                                             <  SRR19973069.3193475/150‑1 (MQ=60)
                                          ATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCA                                                                                                           <  SRR19973069.3713874/150‑1 (MQ=60)
                                          ATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCA                                                                                                           <  SRR19973069.3713868/150‑1 (MQ=60)
                                          ATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCA                                                                                                           <  SRR19973069.3215970/150‑1 (MQ=60)
                                          ATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCA                                                                                                           <  SRR19973069.3215980/150‑1 (MQ=60)
                                          ATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCA                                                                                                           <  SRR19973069.3215985/150‑1 (MQ=60)
                                            TTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGG                                                                                                         <  SRR19973069.2404221/150‑1 (MQ=60)
                                              GGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGC                                                                                                       <  SRR19973069.3839208/150‑1 (MQ=60)
                                               GTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCG                                                                                                      >  SRR19973069.3493200/1‑150 (MQ=60)
                                                TGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGA                                                                                                     >  SRR19973069.2048286/1‑150 (MQ=60)
                                                TGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGA                                                                                                     >  SRR19973069.2049266/1‑150 (MQ=60)
                                                TGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGA                                                                                                     >  SRR19973069.2049294/1‑150 (MQ=60)
                                                  AGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAG                                                                                                   <  SRR19973069.1579626/150‑1 (MQ=60)
                                                  AGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAG                                                                                                   <  SRR19973069.1579805/150‑1 (MQ=60)
                                                  AGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAG                                                                                                   <  SRR19973069.1580315/150‑1 (MQ=60)
                                                      GAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGG                                                                                               <  SRR19973069.2028818/150‑1 (MQ=60)
                                                       AAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGA                                                                                              >  SRR19973069.3486231/1‑150 (MQ=60)
                                                         AAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATC                                                                                            >  SRR19973069.2036503/1‑150 (MQ=60)
                                                         AAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATC                                                                                            >  SRR19973069.307040/1‑150 (MQ=60)
                                                         AAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATC                                                                                            >  SRR19973069.307234/1‑150 (MQ=60)
                                                          AGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCG                                                                                           <  SRR19973069.1120623/150‑1 (MQ=60)
                                                            CGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCT                                                                                         <  SRR19973069.1472974/150‑1 (MQ=60)
                                                              TGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGG                                                                                       <  SRR19973069.2574986/150‑1 (MQ=60)
                                                                    CAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTG                                                                                 <  SRR19973069.127546/150‑1 (MQ=60)
                                                                     AACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAATCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGA                                                                                >  SRR19973069.2735329/1‑150 (MQ=60)
                                                                        CGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAA                                                                             >  SRR19973069.2995273/1‑150 (MQ=60)
                                                                            AGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTG                                                                         <  SRR19973069.2290928/150‑1 (MQ=60)
                                                                            AGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTG                                                                         <  SRR19973069.2290013/150‑1 (MQ=60)
                                                                            AGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTG                                                                         <  SRR19973069.1668443/150‑1 (MQ=60)
                                                                              TTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTC                                                                       <  SRR19973069.12606/150‑1 (MQ=60)
                                                                              TTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTC                                                                       <  SRR19973069.11571/150‑1 (MQ=60)
                                                                                   TGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAAT                                                                  <  SRR19973069.395617/150‑1 (MQ=60)
                                                                                    GGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATG                                                                 <  SRR19973069.1773102/150‑1 (MQ=60)
                                                                                       AAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGTCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCA                                                              >  SRR19973069.1088957/1‑150 (MQ=60)
                                                                                        AGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAG                                                             <  SRR19973069.62403/150‑1 (MQ=60)
                                                                                           GGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAA                                                          >  SRR19973069.1743051/1‑150 (MQ=60)
                                                                                           GGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAA                                                          >  SRR19973069.3647926/1‑150 (MQ=60)
                                                                                             CGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGT                                                        >  SRR19973069.3682407/1‑150 (MQ=60)
                                                                                              GTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTG                                                       >  SRR19973069.3458264/1‑150 (MQ=60)
                                                                                              GTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTG                                                       <  SRR19973069.2145623/150‑1 (MQ=60)
                                                                                                TGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTG                                                     >  SRR19973069.2806752/1‑150 (MQ=60)
                                                                                                TGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTG                                                     >  SRR19973069.2806838/1‑150 (MQ=60)
                                                                                                   CGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTT                                                  <  SRR19973069.3525255/150‑1 (MQ=60)
                                                                                                     CCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCG                                                >  SRR19973069.2427184/1‑150 (MQ=60)
                                                                                                            CGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCG                                         >  SRR19973069.1858954/1‑150 (MQ=60)
                                                                                                            CGTGGTGGTGATGGATTACAGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCG                                         <  SRR19973069.1880348/150‑1 (MQ=60)
                                                                                                               GGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGG                                      >  SRR19973069.3415726/1‑150 (MQ=60)
                                                                                                                GTGGTGATGGATTACGGTGATTTCAGCGTTACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGC                                     >  SRR19973069.1106615/1‑150 (MQ=60)
                                                                                                                  GGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAG                                   <  SRR19973069.1923169/150‑1 (MQ=60)
                                                                                                                     GATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCA                                >  SRR19973069.808094/1‑150 (MQ=60)
                                                                                                                        GGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAAT                             <  SRR19973069.2679718/150‑1 (MQ=60)
                                                                                                                        GGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAAT                             <  SRR19973069.1584197/150‑1 (MQ=60)
                                                                                                                        GGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAAT                             <  SRR19973069.2679631/150‑1 (MQ=60)
                                                                                                                         GATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATG                            <  SRR19973069.914049/150‑1 (MQ=60)
                                                                                                                         GATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATG                            >  SRR19973069.2682650/1‑150 (MQ=60)
                                                                                                                         GATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATG                            <  SRR19973069.914181/150‑1 (MQ=60)
                                                                                                                            TACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAG                         >  SRR19973069.1458159/1‑150 (MQ=60)
                                                                                                                             ACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGG                        >  SRR19973069.722121/1‑150 (MQ=60)
                                                                                                                             ACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGG                        >  SRR19973069.2651952/1‑150 (MQ=60)
                                                                                                                                 TGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCT                    <  SRR19973069.1964693/150‑1 (MQ=60)
                                                                                                                                     TTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACC                <  SRR19973069.114426/150‑1 (MQ=60)
                                                                                                                                       CAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCA              >  SRR19973069.3555516/1‑150 (MQ=60)
                                                                                                                                       CAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCA              <  SRR19973069.2787429/150‑1 (MQ=60)
                                                                                                                                       CAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCA              <  SRR19973069.1699702/150‑1 (MQ=60)
                                                                                                                                       CAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCA              <  SRR19973069.2790166/150‑1 (MQ=60)
                                                                                                                                          CGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCC           >  SRR19973069.3484514/1‑150 (MQ=60)
                                                                                                                                           GTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCG          >  SRR19973069.269070/1‑150 (MQ=60)
                                                                                                                                           GTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCG          >  SRR19973069.266745/1‑150 (MQ=60)
                                                                                                                                           GTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCG          >  SRR19973069.2023777/1‑150 (MQ=60)
                                                                                                                                           GTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCG          >  SRR19973069.2023865/1‑150 (MQ=60)
                                                                                                                                           GTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCG          >  SRR19973069.2023672/1‑150 (MQ=60)
                                                                                                                                           GTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCG          >  SRR19973069.2023755/1‑150 (MQ=60)
                                                                                                                                             CACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCA        >  SRR19973069.2990584/1‑150 (MQ=60)
                                                                                                                                             CACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCA        >  SRR19973069.2990585/1‑150 (MQ=60)
                                                                                                                                             CACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCA        >  SRR19973069.2990589/1‑150 (MQ=60)
                                                                                                                                             CACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAATCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCA        >  SRR19973069.3657052/1‑150 (MQ=60)
                                                                                                                                              ACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAG       >  SRR19973069.1270042/1‑150 (MQ=60)
                                                                                                                                                 TTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCAT    >  SRR19973069.280424/1‑150 (MQ=60)
                                                                                                                                                  TGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATA   <  SRR19973069.1026953/150‑1 (MQ=60)
                                                                                                                                                   GCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATAT  <  SRR19973069.1690985/150‑1 (MQ=60)
                                                                                                                                                   GCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATAT  <  SRR19973069.1691753/150‑1 (MQ=60)
                                                                                                                                                   GCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATAT  <  SRR19973069.176809/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATAT  >  CP009273/3231200‑3231496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑