Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,231,349 A→C 100% Q227P (CAG→CCG)  ygjR → putative NAD(P)‑dependent dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,231,3490AC98.6% 847.8 / ‑2.1 208Q227P (CAG→CCG) ygjRputative NAD(P)‑dependent dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base C (89/116);  total (90/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 208 total aligned reads displayed.

TATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCA  >  CP009273/3231206‑3231493
                                                                                                                                               |                                                                                                                                                
tatCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCAc                                                                                                                                              >  1:1119624/1‑148 (MQ=255)
 atCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGACGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGTTGATTTCAGCGTCACATTGCCGCACt                                                                                                                                             >  2:2378021/1‑148 (MQ=255)
   cGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCa                                                                                                                                           >  2:2455418/1‑148 (MQ=255)
   cGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCa                                                                                                                                           >  2:2183508/1‑148 (MQ=255)
   cGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCa                                                                                                                                           >  2:588068/1‑148 (MQ=255)
   cGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCa                                                                                                                                           >  2:587460/1‑148 (MQ=255)
      tttttACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCaaaa                                                                                                                                        >  1:937890/1‑148 (MQ=255)
      tttttACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCaaaa                                                                                                                                        >  1:1134333/1‑148 (MQ=255)
      tttttACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCaaaa                                                                                                                                        >  1:2884992/1‑148 (MQ=255)
          tACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCa                                                                                                                                    >  1:437710/1‑148 (MQ=255)
           aCTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAg                                                                                                                                   <  1:1558083/148‑1 (MQ=255)
            cTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAATCGTGCAGGCAACCCCCAGTTTGCTGGCAAGCGGGGTTGACTCCCCGGGCGTGGGTGTGTTGGTTTAAGGTGATTTAACCGTCACCTTGCCGCACTAAAAAGTCAGt                                                                                                                                  >  1:677934/1‑148 (MQ=255)
             tGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTg                                                                                                                                 <  1:1899577/148‑1 (MQ=255)
              gTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGa                                                                                                                                <  1:709045/148‑1 (MQ=255)
              gTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGa                                                                                                                                <  1:1315943/148‑1 (MQ=255)
               tCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGAt                                                                                                                               >  2:3028722/1‑148 (MQ=255)
                cTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGAtt                                                                                                                              <  1:2243145/148‑1 (MQ=255)
                  ggCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCt                                                                                                                            >  1:3009711/1‑148 (MQ=255)
                   gCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTg                                                                                                                           >  2:2752991/1‑148 (MQ=255)
                     gTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTc                                                                                                                         <  2:3341094/148‑1 (MQ=255)
                      tCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTcc                                                                                                                        <  2:2242681/148‑1 (MQ=255)
                      tCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTcc                                                                                                                        <  2:2959880/148‑1 (MQ=255)
                         gcggTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTgg                                                                                                                     <  1:2341876/148‑1 (MQ=255)
                            gTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGgcga                                                                                                                  >  2:835902/1‑148 (MQ=255)
                               gCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGgcgagcg                                                                                                               <  1:3157918/148‑1 (MQ=255)
                               gCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGgcgagcg                                                                                                               >  2:1110873/1‑148 (MQ=255)
                                   tATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGAt                                                                                                           >  1:3221814/1‑148 (MQ=255)
                                   tATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGAt                                                                                                           <  2:2041388/148‑1 (MQ=255)
                                    aTTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGAtt                                                                                                          <  2:2803747/148‑1 (MQ=255)
                                       tGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAg                                                                                                       <  2:1710447/148‑1 (MQ=255)
                                       tGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAg                                                                                                       <  1:271518/148‑1 (MQ=255)
                                       tGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAg                                                                                                       <  2:1903388/148‑1 (MQ=255)
                                       tGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCGGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAg                                                                                                       <  2:2508388/148‑1 (MQ=255)
                                        ggTGAGCCGAAACGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAgg                                                                                                      <  1:1239836/148‑1 (MQ=255)
                                           gAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCg                                                                                                   >  1:3090047/1‑148 (MQ=255)
                                            aGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGa                                                                                                  >  1:1031063/1‑148 (MQ=255)
                                            aGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGa                                                                                                  <  2:481482/148‑1 (MQ=255)
                                              ccGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAg                                                                                                <  2:747912/148‑1 (MQ=255)
                                               cGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGc                                                                                               <  2:310512/148‑1 (MQ=255)
                                               cGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGc                                                                                               <  2:1156179/148‑1 (MQ=255)
                                                 aaaaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGACGCAg                                                                                             >  2:2261738/1‑148 (MQ=255)
                                                 aaaaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAg                                                                                             >  1:2738259/1‑148 (MQ=255)
                                                 aaaaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAg                                                                                             >  1:2741479/1‑148 (MQ=255)
                                                   aaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGa                                                                                           <  2:1563421/148‑1 (MQ=255)
                                                   aaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCGTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGa                                                                                           >  1:2365715/1‑148 (MQ=255)
                                                    aGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGAt                                                                                          >  1:2477410/1‑148 (MQ=255)
                                                        tGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCt                                                                                      <  1:1597068/148‑1 (MQ=255)
                                                        tGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCt                                                                                      >  1:2195348/1‑148 (MQ=255)
                                                           aGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGt                                                                                   <  1:880164/148‑1 (MQ=255)
                                                            ggCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTg                                                                                  >  2:3213295/1‑148 (MQ=255)
                                                            ggCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTg                                                                                  >  2:3497606/1‑148 (MQ=255)
                                                            ggCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTg                                                                                  <  2:655697/148‑1 (MQ=255)
                                                             gCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGa                                                                                 <  2:21486/148‑1 (MQ=255)
                                                             gCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGa                                                                                 >  2:1864165/1‑148 (MQ=255)
                                                              cAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAt                                                                                >  2:2714212/1‑148 (MQ=255)
                                                              cAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAt                                                                                <  1:1112146/148‑1 (MQ=255)
                                                               aaCCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAtt                                                                               <  1:781059/148‑1 (MQ=255)
                                                               aaCCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAtt                                                                               <  1:486255/148‑1 (MQ=255)
                                                                   gCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGaaa                                                                           <  2:1645802/148‑1 (MQ=255)
                                                                     cAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGaaaaa                                                                         >  2:130472/1‑148 (MQ=255)
                                                                       gTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACt                                                                       >  1:3455879/1‑148 (MQ=255)
                                                                        tttGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTg                                                                      >  2:293394/1‑148 (MQ=255)
                                                                         ttGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTTCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGCTTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGt                                                                     >  1:3421993/1‑148 (MQ=255)
                                                                             gggCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGa                                                                 <  2:18228/147‑1 (MQ=255)
                                                                               gCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAAt                                                               <  1:2627971/148‑1 (MQ=255)
                                                                                cAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATg                                                              >  2:854220/1‑148 (MQ=255)
                                                                                  aGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGcc                                                            <  1:1372099/148‑1 (MQ=255)
                                                                                   gcggcgTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCa                                                           >  1:1745471/1‑148 (MQ=255)
                                                                                   gcggcgTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCa                                                           >  1:2555502/1‑148 (MQ=255)
                                                                                    cggcgTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAg                                                          >  2:2573490/1‑148 (MQ=255)
                                                                                      gcgTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGaa                                                        >  1:1886987/1‑148 (MQ=255)
                                                                                       cgTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGaaa                                                       <  1:1430751/148‑1 (MQ=255)
                                                                                         ttGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAgt                                                     <  1:658052/148‑1 (MQ=255)
                                                                                         ttGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAgt                                                     <  2:1666118/148‑1 (MQ=255)
                                                                                          tGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAgtg                                                    <  1:824697/148‑1 (MQ=255)
                                                                                          tGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAgtg                                                    <  1:1855586/148‑1 (MQ=255)
                                                                                            aCGCCCAGGGCGTGGTTGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGtgtg                                                  <  2:2741479/148‑1 (MQ=255)
                                                                                            aCGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGtgtg                                                  >  2:1115742/1‑148 (MQ=255)
                                                                                            aCGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGtgtg                                                  <  2:2738259/148‑1 (MQ=255)
                                                                                             cGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGc                                                 <  2:1612938/148‑1 (MQ=255)
                                                                                             cGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGc                                                 >  1:1625960/1‑148 (MQ=255)
                                                                                              gCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCt                                                <  2:2227055/148‑1 (MQ=255)
                                                                                               cccAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCtt                                               <  2:1258441/148‑1 (MQ=255)
                                                                                               cccAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCtt                                               <  1:136161/148‑1 (MQ=255)
                                                                                                  agtgCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGt                                            <  2:448601/145‑1 (MQ=255)
                                                                                                  aGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGt                                            >  1:1318672/1‑148 (MQ=255)
                                                                                                  aGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGt                                            <  1:1415468/148‑1 (MQ=255)
                                                                                                    ggCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGc                                          >  2:2963253/1‑148 (MQ=255)
                                                                                                           tggtgATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTgg                                   <  2:2865163/148‑1 (MQ=255)
                                                                                                               gATGGATTACGGTGATTTCAGCGCCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGc                               <  1:2252430/148‑1 (MQ=255)
                                                                                                                   gATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCaaa                           <  1:200985/148‑1 (MQ=255)
                                                                                                                   gATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCaaa                           >  1:1478753/1‑148 (MQ=255)
                                                                                                                     ttACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATg                         >  1:977917/1‑148 (MQ=255)
                                                                                                                      tACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGc                        >  1:2807603/1‑148 (MQ=255)
                                                                                                                         ggTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAgg                     <  1:130472/148‑1 (MQ=255)
                                                                                                                                tCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCAc              <  1:3209636/148‑1 (MQ=255)
                                                                                                                                  aGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCAccc            >  1:1113849/1‑148 (MQ=255)
                                                                                                                                       cACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCg       >  1:369542/1‑148 (MQ=255)
                                                                                                                                         ccTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgca     <  1:3497606/148‑1 (MQ=255)
                                                                                                                                            tGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgcagca  <  2:2884992/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
TATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCA  >  CP009273/3231206‑3231493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTA  >  CP009273/3231200‑3231498
                                                                                                                                                     |                                                                                                                                                     
TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCC                                                                                                                                                       <  SRR19973065.1422653/150‑1 (MQ=60)
TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCC                                                                                                                                                       <  SRR19973065.1422685/150‑1 (MQ=60)
TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCC                                                                                                                                                       <  SRR19973065.3077040/150‑1 (MQ=60)
 ATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGATCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTAAGCGTCACCTTGCCG                                                                                                                                                      >  SRR19973065.901622/1‑150 (MQ=60)
 ATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCG                                                                                                                                                      >  SRR19973065.2774129/1‑150 (MQ=60)
 ATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCACGGCGGGGTGGTGATGGATTACGGTGATTTCACCGTCACCATGCCG                                                                                                                                                      >  SRR19973065.1858146/1‑150 (MQ=60)
    GATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCAC                                                                                                                                                   >  SRR19973065.1126927/1‑150 (MQ=60)
      TATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTC                                                                                                                                                 >  SRR19973065.855473/1‑150 (MQ=60)
          GGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAA                                                                                                                                             >  SRR19973065.1141685/1‑150 (MQ=60)
          GGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAA                                                                                                                                             >  SRR19973065.2902638/1‑150 (MQ=60)
          GGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGGGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAA                                                                                                                                             >  SRR19973065.944090/1‑150 (MQ=60)
              TTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCA                                                                                                                                         >  SRR19973065.440747/1‑150 (MQ=60)
                TACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAATCGTGCAGGCAACCCCCAGTTTGCTGGCAAGCGGGGTTGACTCCCCGGGCGTGGGTGTGTTGGTTTAAGGTGATTTAACCGTCACCTTGCCGCACTAAAAAGTCAGT                                                                                                                                       >  SRR19973065.682452/1‑150 (MQ=60)
                TACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGT                                                                                                                                       <  SRR19973065.2333577/150‑1 (MQ=60)
                TACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGT                                                                                                                                       <  SRR19973065.1119922/150‑1 (MQ=60)
                 ACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTG                                                                                                                                      <  SRR19973065.1568423/150‑1 (MQ=60)
                   TGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGAT                                                                                                                                    <  SRR19973065.1912059/150‑1 (MQ=60)
                   TGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCACGGCGGGGTGGTGATGGATTACGGTGATTTCAGCGTCCCCTTGCCGCCCTCAAAAGTCCGTGAT                                                                                                                                    >  SRR19973065.920296/1‑150 (MQ=60)
                    GTCTGGCGTCGGCGGTGGCGTTATTTTGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATT                                                                                                                                   <  SRR19973065.3019124/150‑1 (MQ=60)
                    GTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCCGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTAAGCGTCACCTTGCCGCACTCCAAAGTCAGTGATT                                                                                                                                   >  SRR19973065.392490/1‑150 (MQ=60)
                    GTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATT                                                                                                                                   <  SRR19973065.1264480/150‑1 (MQ=60)
                    GTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATT                                                                                                                                   <  SRR19973065.2821307/150‑1 (MQ=60)
                    GTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATT                                                                                                                                   <  SRR19973065.713783/150‑1 (MQ=60)
                    GTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATT                                                                                                                                   <  SRR19973065.1324647/150‑1 (MQ=60)
                    GTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATT                                                                                                                                   <  SRR19973065.1324651/150‑1 (MQ=60)
                      CTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCT                                                                                                                                 <  SRR19973065.2257392/150‑1 (MQ=60)
                      CTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCT                                                                                                                                 >  SRR19973065.3028142/1‑150 (MQ=60)
                       TGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGCTGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTG                                                                                                                                >  SRR19973065.467149/1‑150 (MQ=60)
                           cggtttgtgcctgagcgtttgaagctCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCT                                                                                                                            <  SRR19973065.1979918/124‑1 (MQ=60)
                           cggtttgtgcctgagcgtttgaagctCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCT                                                                                                                            <  SRR19973065.662233/124‑1 (MQ=60)
                             CGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGG                                                                                                                          <  SRR19973065.1138686/150‑1 (MQ=60)
                               GCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCG                                                                                                                        <  SRR19973065.2356494/150‑1 (MQ=60)
                               GCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCG                                                                                                                        <  SRR19973065.582797/150‑1 (MQ=60)
                               GCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCG                                                                                                                        <  SRR19973065.1365408/150‑1 (MQ=60)
                                    GGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGA                                                                                                                   >  SRR19973065.2871423/1‑150 (MQ=60)
                                     GCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAG                                                                                                                  <  SRR19973065.3177063/150‑1 (MQ=60)
                                     GCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAG                                                                                                                  <  SRR19973065.3177798/150‑1 (MQ=60)
                                       GTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGAT                                                                                                                >  SRR19973065.3241173/1‑150 (MQ=60)
                                          ATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCA                                                                                                             >  SRR19973065.961963/1‑150 (MQ=60)
                                             TGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGG                                                                                                          <  SRR19973065.273350/150‑1 (MQ=60)
                                             TGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGG                                                                                                          <  SRR19973065.2370307/150‑1 (MQ=60)
                                              GGTGAGCCGAAACGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGC                                                                                                         <  SRR19973065.1247980/150‑1 (MQ=60)
                                              GGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGC                                                                                                         <  SRR19973065.364378/150‑1 (MQ=60)
                                               GTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCG                                                                                                        >  SRR19973065.3108911/1‑150 (MQ=60)
                                                TGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGA                                                                                                       >  SRR19973065.1037934/1‑150 (MQ=60)
                                                TGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGA                                                                                                       >  SRR19973065.1037893/1‑150 (MQ=60)
                                                  AGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAG                                                                                                     >  SRR19973065.3338107/1‑150 (MQ=60)
                                                     CGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAG                                                                                                  >  SRR19973065.2755293/1‑150 (MQ=60)
                                                     CGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAG                                                                                                  >  SRR19973065.2755474/1‑150 (MQ=60)
                                                     CGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAG                                                                                                  >  SRR19973065.2758528/1‑150 (MQ=60)
                                                       AAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCGTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGA                                                                                                >  SRR19973065.2380455/1‑150 (MQ=60)
                                                        AAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGAT                                                                                               >  SRR19973065.2493008/1‑150 (MQ=60)
                                                        AAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGAT                                                                                               <  SRR19973065.1803174/150‑1 (MQ=60)
                                                         AAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATC                                                                                              <  SRR19973065.2531389/150‑1 (MQ=60)
                                                         AAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATC                                                                                              >  SRR19973065.1322495/1‑150 (MQ=60)
                                                         AAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGGGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATC                                                                                              >  SRR19973065.3122740/1‑150 (MQ=60)
                                                            CGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCT                                                                                           >  SRR19973065.2209416/1‑150 (MQ=60)
                                                            CGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCT                                                                                           <  SRR19973065.2684149/150‑1 (MQ=60)
                                                            CGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCT                                                                                           <  SRR19973065.2635439/150‑1 (MQ=60)
                                                              TGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGG                                                                                         <  SRR19973065.1607736/150‑1 (MQ=60)
                                                                CAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTG                                                                                       >  SRR19973065.2751258/1‑150 (MQ=60)
                                                                 AGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGA                                                                                      <  SRR19973065.885771/150‑1 (MQ=60)
                                                                 AGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGA                                                                                      <  SRR19973065.1461214/150‑1 (MQ=60)
                                                                    CAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTG                                                                                   <  SRR19973065.1119414/150‑1 (MQ=60)
                                                                     AACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGA                                                                                  <  SRR19973065.786179/150‑1 (MQ=60)
                                                                     AACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGA                                                                                  <  SRR19973065.786243/150‑1 (MQ=60)
                                                                     AACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGA                                                                                  <  SRR19973065.489550/150‑1 (MQ=60)
                                                                           CAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACT                                                                            <  SRR19973065.1650244/150‑1 (MQ=60)
                                                                           CAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACT                                                                            >  SRR19973065.3476942/1‑150 (MQ=60)
                                                                             GTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTTCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGCTTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGT                                                                          >  SRR19973065.3442892/1‑150 (MQ=60)
                                                                                     GCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGC                                                                  <  SRR19973065.2644227/150‑1 (MQ=60)
                                                                                       AAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCA                                                                >  SRR19973065.1756982/1‑150 (MQ=60)
                                                                                       AAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCA                                                                >  SRR19973065.2571489/1‑150 (MQ=60)
                                                                                       AAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCA                                                                >  SRR19973065.3503847/1‑150 (MQ=60)
                                                                                        AGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAG                                                               <  SRR19973065.1381155/150‑1 (MQ=60)
                                                                                        AGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAG                                                               <  SRR19973065.1380663/150‑1 (MQ=60)
                                                                                          CGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAA                                                             >  SRR19973065.1899403/1‑150 (MQ=60)
                                                                                          CGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAA                                                             >  SRR19973065.1591703/1‑150 (MQ=60)
                                                                                            GCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAG                                                           <  SRR19973065.1543246/150‑1 (MQ=60)
                                                                                             CGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGT                                                          <  SRR19973065.1440040/150‑1 (MQ=60)
                                                                                             CGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGT                                                          <  SRR19973065.1440057/150‑1 (MQ=60)
                                                                                             CGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGT                                                          <  SRR19973065.2277895/150‑1 (MQ=60)
                                                                                               TTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGT                                                        <  SRR19973065.662397/150‑1 (MQ=60)
                                                                                               TTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGT                                                        <  SRR19973065.662398/150‑1 (MQ=60)
                                                                                                TGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTG                                                       <  SRR19973065.830106/150‑1 (MQ=60)
                                                                                                TGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTG                                                       <  SRR19973065.829823/150‑1 (MQ=60)
                                                                                                TGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTG                                                       <  SRR19973065.1867800/150‑1 (MQ=60)
                                                                                                TGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTG                                                       <  SRR19973065.1867866/150‑1 (MQ=60)
                                                                                                TGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTG                                                       <  SRR19973065.829832/150‑1 (MQ=60)
                                                                                                 GACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGC                                                      >  SRR19973065.1636652/1‑150 (MQ=60)
                                                                                                 GACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGC                                                      >  SRR19973065.1636855/1‑150 (MQ=60)
                                                                                                   CGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTT                                                    <  SRR19973065.1443427/150‑1 (MQ=60)
                                                                                                     CCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCG                                                  <  SRR19973065.137326/150‑1 (MQ=60)
                                                                                                      CCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGT                                                 >  SRR19973065.1327395/1‑150 (MQ=60)
                                                                                                       CAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTG                                                >  SRR19973065.1187451/1‑150 (MQ=60)
                                                                                                        AGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGC                                               <  SRR19973065.1424713/150‑1 (MQ=60)
                                                                                                        AGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGC                                               <  SRR19973065.1424709/150‑1 (MQ=60)
                                                                                                               GGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGG                                        >  SRR19973065.2224613/1‑150 (MQ=60)
                                                                                                                  GGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAG                                     <  SRR19973065.2182981/150‑1 (MQ=60)
                                                                                                                   GTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGC                                    >  SRR19973065.2412498/1‑150 (MQ=60)
                                                                                                                     GATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCA                                  <  SRR19973065.2266663/150‑1 (MQ=60)
                                                                                                                     GATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCA                                  <  SRR19973065.3047281/150‑1 (MQ=60)
                                                                                                                     GATGGATTACGGTGATTTCAGCGCCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCA                                  <  SRR19973065.2266703/150‑1 (MQ=60)
                                                                                                                       TGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAA                                >  SRR19973065.3231299/1‑150 (MQ=60)
                                                                                                                       TGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAA                                >  SRR19973065.1488205/1‑150 (MQ=60)
                                                                                                                        GGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAAT                               <  SRR19973065.70583/150‑1 (MQ=60)
                                                                                                                         GATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATG                              >  SRR19973065.984408/1‑150 (MQ=60)
                                                                                                                         GATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATG                              <  SRR19973065.202537/150‑1 (MQ=60)
                                                                                                                         GATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATG                              <  SRR19973065.202531/150‑1 (MQ=60)
                                                                                                                          ATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGC                             >  SRR19973065.2824973/1‑150 (MQ=60)
                                                                                                                               GGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGAT                        <  SRR19973065.131595/150‑1 (MQ=60)
                                                                                                                                  GATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTC                     <  SRR19973065.3447135/150‑1 (MQ=60)
                                                                                                                                      TCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCC                 <  SRR19973065.3228947/150‑1 (MQ=60)
                                                                                                                                      TCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCC                 >  SRR19973065.1121127/1‑150 (MQ=60)
                                                                                                                                      TCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCC                 >  SRR19973065.1906389/1‑150 (MQ=60)
                                                                                                                                           GTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCG            >  SRR19973065.372130/1‑150 (MQ=60)
                                                                                                                                             CACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCA          >  SRR19973065.1121261/1‑150 (MQ=60)
                                                                                                                                               CCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGC        <  SRR19973065.3518858/150‑1 (MQ=60)
                                                                                                                                                 TTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCAT      <  SRR19973065.3190003/150‑1 (MQ=60)
                                                                                                                                                   GCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATAT    <  SRR19973065.2616186/150‑1 (MQ=60)
                                                                                                                                                   GCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATAT    <  SRR19973065.858714/150‑1 (MQ=60)
                                                                                                                                                   GCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATAT    <  SRR19973065.2616192/150‑1 (MQ=60)
                                                                                                                                                     CGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTA  >  SRR19973065.1253290/1‑150 (MQ=60)
                                                                                                                                                     CGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTA  <  SRR19973065.2192453/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTA  >  CP009273/3231200‑3231498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑