Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,231,349 A→C 100% Q227P (CAG→CCG)  ygjR → putative NAD(P)‑dependent dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,231,3490AC98.4% 741.8 / ‑2.5 185Q227P (CAG→CCG) ygjRputative NAD(P)‑dependent dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base C (93/89);  total (94/91)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 187 total aligned reads displayed.

GATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATA  >  CP009273/3231204‑3231495
                                                                                                                                                 |                                                                                                                                                  
  tatCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCAc                                                                                                                                                >  1:1266630/1‑148 (MQ=255)
       gTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCaaa                                                                                                                                           <  2:2918633/148‑1 (MQ=255)
        tttttACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCaaaa                                                                                                                                          <  1:657943/148‑1 (MQ=255)
         ttttACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAg                                                                                                                                         >  2:2181301/1‑148 (MQ=255)
            tACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCa                                                                                                                                      <  1:1565265/148‑1 (MQ=255)
             aCTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAg                                                                                                                                     <  1:2953374/148‑1 (MQ=255)
             aCTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACACAAAAGTCAg                                                                                                                                     >  1:1137787/1‑148 (MQ=255)
               tGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTg                                                                                                                                   >  2:2256190/1‑148 (MQ=255)
               tGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTg                                                                                                                                   >  2:396596/1‑148 (MQ=255)
                gTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGa                                                                                                                                  <  1:3357801/148‑1 (MQ=255)
                gTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGa                                                                                                                                  <  1:2196394/148‑1 (MQ=255)
                gTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGGGGTGGTGATGGATTACGGTGATTTCAGCGTCACCATGCCGCACTCAAAACTCAGTGa                                                                                                                                  >  2:308332/1‑148 (MQ=255)
                 tCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGAt                                                                                                                                 <  2:2536367/148‑1 (MQ=255)
                  cTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGAtt                                                                                                                                <  2:1086807/148‑1 (MQ=255)
                  cTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGAtt                                                                                                                                <  1:144649/148‑1 (MQ=255)
                       gTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTc                                                                                                                           <  2:335806/148‑1 (MQ=255)
                       gTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTc                                                                                                                           <  2:1833167/148‑1 (MQ=255)
                          ggcggTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTg                                                                                                                        <  1:2097080/148‑1 (MQ=255)
                             ggTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGgcg                                                                                                                     <  1:200694/148‑1 (MQ=255)
                               tGGCGTTATTTGGTGAGCCGAAAATCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGgcgag                                                                                                                   >  1:2822376/1‑148 (MQ=255)
                                  cGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGgcgagcga                                                                                                                <  1:579425/148‑1 (MQ=255)
                                   gTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGcgagcgag                                                                                                               <  2:38628/148‑1 (MQ=255)
                                   gTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGcgagcgag                                                                                                               <  2:2085167/148‑1 (MQ=255)
                                   gTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGcgagcgag                                                                                                               <  1:523333/148‑1 (MQ=255)
                                    ttATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCgaga                                                                                                              <  1:2212422/148‑1 (MQ=255)
                                          ggTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAgg                                                                                                        <  2:737519/148‑1 (MQ=255)
                                          ggTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAgg                                                                                                        <  1:3039760/148‑1 (MQ=255)
                                           gTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAggg                                                                                                       >  2:211416/1‑148 (MQ=255)
                                           gTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAggg                                                                                                       <  2:1126291/148‑1 (MQ=255)
                                           gTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGAGATTCTGTCCTGGCGAGCGAGATTCAggg                                                                                                       >  2:123291/1‑148 (MQ=255)
                                              aGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGa                                                                                                    <  1:1496319/148‑1 (MQ=255)
                                                  gAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCa                                                                                                >  2:812091/1‑148 (MQ=255)
                                                    aaaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAgg                                                                                              <  1:2724207/148‑1 (MQ=255)
                                                    aaaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAgg                                                                                              >  2:3133202/1‑148 (MQ=255)
                                                     aaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGa                                                                                             <  2:1867572/148‑1 (MQ=255)
                                                     aaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGa                                                                                             >  1:3169641/1‑148 (MQ=255)
                                                     aaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGa                                                                                             <  1:395331/148‑1 (MQ=255)
                                                      aGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGAt                                                                                            >  1:2877513/1‑148 (MQ=255)
                                                      aGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGAt                                                                                            >  1:2439436/1‑148 (MQ=255)
                                                        cGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCCAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCg                                                                                          >  1:2763330/1‑148 (MQ=255)
                                                        cGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCg                                                                                          <  1:1052806/148‑1 (MQ=255)
                                                        cGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCg                                                                                          >  1:981855/1‑148 (MQ=255)
                                                        cGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCg                                                                                          <  1:762373/148‑1 (MQ=255)
                                                        cGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCg                                                                                          <  1:384574/148‑1 (MQ=255)
                                                         gTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGc                                                                                         <  1:2301761/148‑1 (MQ=255)
                                                         gTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGc                                                                                         <  1:1275557/148‑1 (MQ=255)
                                                           gCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTg                                                                                       >  1:999117/1‑148 (MQ=255)
                                                             aGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGt                                                                                     >  2:2589534/1‑148 (MQ=255)
                                                             aGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGt                                                                                     <  1:3244085/148‑1 (MQ=255)
                                                             aGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGt                                                                                     <  2:1110756/148‑1 (MQ=255)
                                                              ggCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTg                                                                                    >  1:3082288/1‑148 (MQ=255)
                                                                cAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAt                                                                                  >  2:2587581/1‑148 (MQ=255)
                                                                cAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAt                                                                                  <  2:1428772/148‑1 (MQ=255)
                                                                cAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAt                                                                                  <  2:922024/148‑1 (MQ=255)
                                                                 aaCCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAtt                                                                                 >  2:1276729/1‑148 (MQ=255)
                                                                    cGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGaa                                                                              >  2:3467219/1‑148 (MQ=255)
                                                                      ccAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGaaaa                                                                            >  2:3461827/1‑148 (MQ=255)
                                                                      ccAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGaaaa                                                                            <  2:297071/148‑1 (MQ=255)
                                                                         gTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACt                                                                         >  1:3294952/1‑148 (MQ=255)
                                                                          tttGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTg                                                                        >  2:1922168/1‑148 (MQ=255)
                                                                           ttGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGt                                                                       >  2:2122427/1‑148 (MQ=255)
                                                                           ttGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGt                                                                       >  1:2444134/1‑148 (MQ=255)
                                                                           ttGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGt                                                                       <  2:1598494/148‑1 (MQ=255)
                                                                           ttGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGt                                                                       <  2:3095468/148‑1 (MQ=255)
                                                                           ttGCTGGCAAGCGGCGTTGACGCCCAGGGCGGGGGGGTGATGGATTACGGTGATTTAAGCGTCACCTTTCCGCACAAAAAAGTCAGTTATTCTTTCCTGGCCAGCGAGATTCAGGGCGAAGCAGGATCCCTGGTGTTTTAAAAACttt                                                                       >  1:313548/1‑146 (MQ=255)
                                                                                  cAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATg                                                                >  2:3401687/1‑148 (MQ=255)
                                                                                  cAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATg                                                                <  1:2378965/148‑1 (MQ=255)
                                                                                  cAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATg                                                                <  1:2378969/148‑1 (MQ=255)
                                                                                           ttGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAgt                                                       <  2:2630883/148‑1 (MQ=255)
                                                                                            tGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAgtg                                                      <  1:1272100/148‑1 (MQ=255)
                                                                                            tGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAgtg                                                      <  1:1034679/148‑1 (MQ=255)
                                                                                            tGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAgtg                                                      <  1:73789/148‑1 (MQ=255)
                                                                                                gCCCAGGGCGTGGTTGTGATGGTTTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGGGAAGCTGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCt                                                  <  2:1383833/148‑1 (MQ=255)
                                                                                                gCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCt                                                  >  1:2178382/1‑148 (MQ=255)
                                                                                                      ggCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGc                                            >  2:2215602/1‑148 (MQ=255)
                                                                                                      ggCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGc                                            >  1:1636506/1‑148 (MQ=255)
                                                                                                      ggCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGc                                            >  2:869209/1‑148 (MQ=255)
                                                                                                      ggCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGc                                            >  2:1466180/1‑148 (MQ=255)
                                                                                                      ggCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGc                                            >  2:3055857/1‑148 (MQ=255)
                                                                                                      ggCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGc                                            >  1:2136283/1‑148 (MQ=255)
                                                                                                                tgATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAGAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAg                                  >  1:2834195/1‑148 (MQ=255)
                                                                                                                tgATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAg                                  >  1:2834309/1‑148 (MQ=255)
                                                                                                                tgATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAg                                  >  1:1496503/1‑148 (MQ=255)
                                                                                                                  aTGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGcc                                >  2:284243/1‑148 (MQ=255)
                                                                                                                  aTGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGcc                                >  2:1999863/1‑148 (MQ=255)
                                                                                                                    ggATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCaa                              >  2:156923/1‑148 (MQ=255)
                                                                                                                    ggATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCaa                              <  2:3219021/148‑1 (MQ=255)
                                                                                                                       ttACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATg                           <  2:2747809/148‑1 (MQ=255)
                                                                                                                          cGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAg                        <  1:812091/148‑1 (MQ=255)
                                                                                                                            gTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCCGGATCGCTGGGGATAGAAAAACAGTCTCAATGCCAGAAAGTGTGCTTCGTGACGCGTGGCAGCCAAATGCAGGa                      >  1:3292097/1‑148 (MQ=255)
                                                                                                                             tGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGAt                     >  1:2364885/1‑148 (MQ=255)
                                                                                                                                 ttCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCa                 >  1:1789442/1‑148 (MQ=255)
                                                                                                                                    aGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCAccc              >  1:2077274/1‑148 (MQ=255)
                                                                                                                                     gCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCa             >  2:1072796/1‑148 (MQ=255)
                                                                                                                                      cGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAg            <  1:1922168/148‑1 (MQ=255)
                                                                                                                                           ccTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgca       >  1:2774695/1‑148 (MQ=255)
                                                                                                                                            cTTGCCGCACTCAAAAGTCTGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgcag      <  2:591054/148‑1 (MQ=255)
                                                                                                                                            cTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgcag      >  2:3291672/1‑148 (MQ=255)
                                                                                                                                              tGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgcagca    >  1:1280111/1‑148 (MQ=255)
                                                                                                                                                cagcaCTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCata  <  2:450326/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATA  >  CP009273/3231204‑3231495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATAT  >  CP009273/3231200‑3231496
                                                                                                                                                     |                                                                                                                                                   
TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCC                                                                                                                                                     <  SRR19973066.3167887/150‑1 (MQ=60)
TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCC                                                                                                                                                     <  SRR19973066.1380558/150‑1 (MQ=60)
 ATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCG                                                                                                                                                    >  SRR19973066.1728991/1‑150 (MQ=60)
 ATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCG                                                                                                                                                    >  SRR19973066.1730611/1‑150 (MQ=60)
 ATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCG                                                                                                                                                    >  SRR19973066.1730546/1‑150 (MQ=60)
    GATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCAC                                                                                                                                                 >  SRR19973066.1275779/1‑150 (MQ=60)
     ATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACT                                                                                                                                                >  SRR19973066.1069670/1‑150 (MQ=60)
      TATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTC                                                                                                                                               >  SRR19973066.392501/1‑150 (MQ=60)
          GGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAA                                                                                                                                           >  SRR19973066.756826/1‑150 (MQ=60)
            TTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGT                                                                                                                                         <  SRR19973066.665523/150‑1 (MQ=60)
            TTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGT                                                                                                                                         <  SRR19973066.662747/150‑1 (MQ=60)
              TTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCA                                                                                                                                       >  SRR19973066.2766297/1‑150 (MQ=60)
               TTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAG                                                                                                                                      >  SRR19973066.369615/1‑150 (MQ=60)
               TTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACACAAAAGTCAG                                                                                                                                      >  SRR19973066.1145930/1‑150 (MQ=60)
                TACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGT                                                                                                                                     <  SRR19973066.1576539/150‑1 (MQ=60)
                 ACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTG                                                                                                                                    <  SRR19973066.2972960/150‑1 (MQ=60)
                    GTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATT                                                                                                                                 <  SRR19973066.2211626/150‑1 (MQ=60)
                    GTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATT                                                                                                                                 <  SRR19973066.2211654/150‑1 (MQ=60)
                    GTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATT                                                                                                                                 <  SRR19973066.3380184/150‑1 (MQ=60)
                     TCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTC                                                                                                                                <  SRR19973066.987453/150‑1 (MQ=60)
                      CTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCT                                                                                                                               <  SRR19973066.146030/150‑1 (MQ=60)
                       TGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTG                                                                                                                              <  SRR19973066.1213600/150‑1 (MQ=60)
                        GGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCCGGGCGGGGGGGTGATTGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTTT                                                                                                                             >  SRR19973066.332194/1‑150 (MQ=60)
                           GTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCT                                                                                                                          <  SRR19973066.3009437/150‑1 (MQ=60)
                              GGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGC                                                                                                                       <  SRR19973066.2112174/150‑1 (MQ=60)
                              GGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGC                                                                                                                       <  SRR19973066.2111516/150‑1 (MQ=60)
                                 GGTGGCGTTATTTGGTGAGCCGAAAATCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAG                                                                                                                    >  SRR19973066.2841344/1‑150 (MQ=60)
                                 GGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAG                                                                                                                    <  SRR19973066.202540/150‑1 (MQ=60)
                                 GGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAG                                                                                                                    <  SRR19973066.202397/150‑1 (MQ=60)
                                   TGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCG                                                                                                                  >  SRR19973066.2577633/1‑150 (MQ=60)
                                    GGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGA                                                                                                                 <  SRR19973066.1284918/150‑1 (MQ=60)
                                    GGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGA                                                                                                                 >  SRR19973066.2204305/1‑150 (MQ=60)
                                      CGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGA                                                                                                               <  SRR19973066.583493/150‑1 (MQ=60)
                                      CGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGA                                                                                                               >  SRR19973066.2249903/1‑150 (MQ=60)
                                       GTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGAT                                                                                                              <  SRR19973066.527172/150‑1 (MQ=60)
                                       GTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGAT                                                                                                              <  SRR19973066.2332142/150‑1 (MQ=60)
                                        TTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATT                                                                                                             <  SRR19973066.2227742/150‑1 (MQ=60)
                                              GGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGC                                                                                                       <  SRR19973066.3060075/150‑1 (MQ=60)
                                              GGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGC                                                                                                       >  SRR19973066.1556152/1‑150 (MQ=60)
                                                  AGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAG                                                                                                   <  SRR19973066.1506763/150‑1 (MQ=60)
                                                  AGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAG                                                                                                   <  SRR19973066.1506861/150‑1 (MQ=60)
                                                       AAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGA                                                                                              >  SRR19973066.3190753/1‑150 (MQ=60)
                                                        AAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGAT                                                                                             >  SRR19973066.2456051/1‑150 (MQ=60)
                                                        AAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGAT                                                                                             >  SRR19973066.2456084/1‑150 (MQ=60)
                                                        AAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGAT                                                                                             <  SRR19973066.2742531/150‑1 (MQ=60)
                                                        AAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGAT                                                                                             >  SRR19973066.2896752/1‑150 (MQ=60)
                                                        AAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGAT                                                                                             >  SRR19973066.2896794/1‑150 (MQ=60)
                                                         AAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATC                                                                                            <  SRR19973066.398444/150‑1 (MQ=60)
                                                         AAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATC                                                                                            <  SRR19973066.175475/150‑1 (MQ=60)
                                                          AGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCCAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCG                                                                                           >  SRR19973066.2781936/1‑150 (MQ=60)
                                                          AGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCG                                                                                           <  SRR19973066.777421/150‑1 (MQ=60)
                                                          AGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCG                                                                                           >  SRR19973066.989098/1‑150 (MQ=60)
                                                            CGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCT                                                                                         <  SRR19973066.387609/150‑1 (MQ=60)
                                                            CGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCT                                                                                         <  SRR19973066.768026/150‑1 (MQ=60)
                                                            CGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCT                                                                                         <  SRR19973066.1060549/150‑1 (MQ=60)
                                                             GTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTG                                                                                        <  SRR19973066.3071195/150‑1 (MQ=60)
                                                             GTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTG                                                                                        >  SRR19973066.1006483/1‑150 (MQ=60)
                                                             GTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTG                                                                                        <  SRR19973066.1284767/150‑1 (MQ=60)
                                                             GTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTG                                                                                        <  SRR19973066.2001621/150‑1 (MQ=60)
                                                             GTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTG                                                                                        <  SRR19973066.2317526/150‑1 (MQ=60)
                                                                CAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTG                                                                                     >  SRR19973066.3102875/1‑150 (MQ=60)
                                                                CAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTG                                                                                     >  SRR19973066.3102895/1‑150 (MQ=60)
                                                                 AGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGA                                                                                    <  SRR19973066.3265479/150‑1 (MQ=60)
                                                                 AGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGA                                                                                    <  SRR19973066.3171202/150‑1 (MQ=60)
                                                                           CAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACT                                                                          >  SRR19973066.3316710/1‑150 (MQ=60)
                                                                           CAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACT                                                                          >  SRR19973066.3316709/1‑150 (MQ=60)
                                                                            AGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTG                                                                         >  SRR19973066.573271/1‑150 (MQ=60)
                                                                             GTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGT                                                                        >  SRR19973066.2460782/1‑150 (MQ=60)
                                                                             GTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGGGGGGGTGATGGATTACGGTGATTTAAGCGTCACCTTTCCGCACAAAAAAGTCAGTTATTCTTTCCTGGCCAGCGAGATTCAGGGCGAAGCAGGATCCCTGGTGTTTTAAAAACTTT                                                                        >  SRR19973066.315865/1‑150 (MQ=60)
                                                                                      CAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCC                                                               <  SRR19973066.2394876/150‑1 (MQ=60)
                                                                                      CAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCC                                                               <  SRR19973066.2395021/150‑1 (MQ=60)
                                                                                      CAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCC                                                               <  SRR19973066.2395025/150‑1 (MQ=60)
                                                                                                TGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTG                                                     <  SRR19973066.74662/150‑1 (MQ=60)
                                                                                                TGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTG                                                     <  SRR19973066.1042294/150‑1 (MQ=60)
                                                                                                TGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTG                                                     <  SRR19973066.1281287/150‑1 (MQ=60)
                                                                                                TGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTG                                                     <  SRR19973066.1281650/150‑1 (MQ=60)
                                                                                                TGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTG                                                     <  SRR19973066.278559/150‑1 (MQ=60)
                                                                                                  ACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCT                                                   >  SRR19973066.2445364/1‑150 (MQ=60)
                                                                                                  ACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCT                                                   >  SRR19973066.2193502/1‑150 (MQ=60)
                                                                                                     CCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCG                                                >  SRR19973066.2823802/1‑150 (MQ=60)
                                                                                                       CAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTG                                              >  SRR19973066.1449536/1‑150 (MQ=60)
                                                                                                        AGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGC                                             >  SRR19973066.1648401/1‑150 (MQ=60)
                                                                                                        AGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGC                                             >  SRR19973066.2151105/1‑150 (MQ=60)
                                                                                                              TGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTG                                       >  SRR19973066.2970307/1‑150 (MQ=60)
                                                                                                              TGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTG                                       <  SRR19973066.1930219/150‑1 (MQ=60)
                                                                                                                 TGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCA                                    <  SRR19973066.990953/150‑1 (MQ=60)
                                                                                                                  GGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAGAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAG                                   >  SRR19973066.2853242/1‑150 (MQ=60)
                                                                                                                  GGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAG                                   >  SRR19973066.2853358/1‑150 (MQ=60)
                                                                                                                  GGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAG                                   >  SRR19973066.1506947/1‑150 (MQ=60)
                                                                                                                  GGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAG                                   >  SRR19973066.2853319/1‑150 (MQ=60)
                                                                                                                     GATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCA                                <  SRR19973066.1277486/150‑1 (MQ=60)
                                                                                                                       TGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAA                              >  SRR19973066.2153185/1‑150 (MQ=60)
                                                                                                                            TACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAG                         <  SRR19973066.3266883/150‑1 (MQ=60)
                                                                                                                              CGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCCGGATCGCTGGGGATAGAAAAACAGTCTCAATGCCAGAAAGTGTGCTTCGTGACGCGTGGCAGCCAAATGCAGGA                       >  SRR19973066.3313816/1‑150 (MQ=60)
                                                                                                                              CGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGA                       <  SRR19973066.818047/150‑1 (MQ=60)
                                                                                                                               GGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGAT                      >  SRR19973066.2380898/1‑150 (MQ=60)
                                                                                                                                 TGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCT                    >  SRR19973066.1624442/1‑150 (MQ=60)
                                                                                                                                   ATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCA                  >  SRR19973066.1802194/1‑150 (MQ=60)
                                                                                                                                      TCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCC               >  SRR19973066.2091627/1‑150 (MQ=60)
                                                                                                                                          CGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCC           <  SRR19973066.1935838/150‑1 (MQ=60)
                                                                                                                                             CACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCA        >  SRR19973066.2793376/1‑150 (MQ=60)
                                                                                                                                             CACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCA        >  SRR19973066.453790/1‑150 (MQ=60)
                                                                                                                                                CTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCA     >  SRR19973066.1289358/1‑150 (MQ=60)
                                                                                                                                                   GCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATAT  <  SRR19973066.2312983/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATAT  >  CP009273/3231200‑3231496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑