Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F534 I1 R1	1235	230.2	7611948	95.1%	7238962	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,231,349	A→C	Q227P (CAG→CCG)	ygjR →	putative NAD(P)‑dependent dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,231,349	0	A	C	100.0%	909.0 / NA	229	Q227P (CAG→CCG)	ygjR	putative NAD(P)‑dependent dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (93/136); total (93/136)

Only 100 of 233 total aligned reads displayed.

TGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATA  >  CP009273/3231202‑3231495

tGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTgccgc                                                                                                                                                  >  2:639329/1‑150 (MQ=255)

 ggATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTgccgca                                                                                                                                                 <  2:3084477/150‑1 (MQ=255)

 ggATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTgccgca                                                                                                                                                 >  1:875614/1‑150 (MQ=255)

   atatCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACt                                                                                                                                               >  2:2035250/1‑150 (MQ=255)

   atatCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACt                                                                                                                                               >  1:3555051/1‑150 (MQ=255)

     atCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCa                                                                                                                                             >  2:785320/1‑150 (MQ=255)

     atCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCa                                                                                                                                             <  2:2267307/150‑1 (MQ=255)

          tttttACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGt                                                                                                                                        >  2:3205016/1‑150 (MQ=255)

          tttttACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGt                                                                                                                                        <  1:3032114/150‑1 (MQ=255)

          tttttACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGt                                                                                                                                        <  1:1239637/150‑1 (MQ=255)

              tACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGt                                                                                                                                    <  2:2902844/150‑1 (MQ=255)

                 tGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGAt                                                                                                                                 <  2:1916663/150‑1 (MQ=255)

                  gTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGAtt                                                                                                                                >  2:1298875/1‑150 (MQ=255)

                  gTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGAtt                                                                                                                                >  1:3165858/1‑150 (MQ=255)

                  gTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGAtt                                                                                                                                <  1:2437023/150‑1 (MQ=255)

                   tCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTc                                                                                                                               >  1:301828/1‑150 (MQ=255)

                   tCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTc                                                                                                                               >  1:2499045/1‑150 (MQ=255)

                    cTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCt                                                                                                                              <  1:2377936/150‑1 (MQ=255)

                    cTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCt                                                                                                                              <  1:3512928/150‑1 (MQ=255)

                      ggCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGt                                                                                                                            <  2:2607397/150‑1 (MQ=255)

                      ggCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGt                                                                                                                            >  2:2948942/1‑150 (MQ=255)

                         gTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCt                                                                                                                         <  1:2942296/150‑1 (MQ=255)

                         gTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCt                                                                                                                         <  2:3098861/150‑1 (MQ=255)

                           cggcggTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTgg                                                                                                                       >  2:625922/1‑150 (MQ=255)

                            ggcggTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGgc                                                                                                                      >  2:2716501/1‑150 (MQ=255)

                                   gCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGcgagcgag                                                                                                               >  1:1757953/1‑150 (MQ=255)

                                   gCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGcgagcgag                                                                                                               >  1:1689577/1‑150 (MQ=255)

                                    cGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCGGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCgaga                                                                                                              >  1:2670027/1‑150 (MQ=255)

                                    cGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCgaga                                                                                                              >  1:2725096/1‑150 (MQ=255)

                                    cGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCgaga                                                                                                              >  2:2487861/1‑150 (MQ=255)

                                    cGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCgaga                                                                                                              >  1:2140680/1‑150 (MQ=255)

                                      ttATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGAtt                                                                                                            <  1:1129289/150‑1 (MQ=255)

                                          ttGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAgg                                                                                                        <  2:123884/150‑1 (MQ=255)

                                           tGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAggg                                                                                                       <  1:3530965/150‑1 (MQ=255)

                                           tGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAggg                                                                                                       >  1:1805215/1‑150 (MQ=255)

                                            ggTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGc                                                                                                      <  2:1097848/150‑1 (MQ=255)

                                            ggTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGc                                                                                                      >  2:3345143/1‑150 (MQ=255)

                                            ggTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGc                                                                                                      <  2:2229900/150‑1 (MQ=255)

                                               gAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGaa                                                                                                   <  1:3108338/150‑1 (MQ=255)

                                                 gCCGAATAGCGTGCAGGCAACCGCCAGTTTGCTGGCTAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGTTTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGc                                                                                                 <  2:40524/150‑1 (MQ=255)

                                                    gAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAgg                                                                                              >  1:548987/1‑150 (MQ=255)

                                                    gAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAgg                                                                                              >  2:2061806/1‑150 (MQ=255)

                                                    gAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAgg                                                                                              <  2:1946045/150‑1 (MQ=255)

                                                    gAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAgg                                                                                              <  1:1426951/150‑1 (MQ=255)

                                                     aaaaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGa                                                                                             >  1:1833729/1‑150 (MQ=255)

                                                      aaaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGAt                                                                                            <  1:936949/150‑1 (MQ=255)

                                                      aaaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGAt                                                                                            >  1:3273761/1‑150 (MQ=255)

                                                       aaGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATc                                                                                           >  2:1757489/1‑150 (MQ=255)

                                                          cGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGTTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGCTTCAGGGCGAAGCAGGATCGCt                                                                                        >  2:2166919/1‑150 (MQ=255)

                                                            tGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTgg                                                                                      >  1:1052655/1‑150 (MQ=255)

                                                             gCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGt                                                                                     <  2:3483804/150‑1 (MQ=255)

                                                             gCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGt                                                                                     >  1:3459774/1‑150 (MQ=255)

                                                              cAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTg                                                                                    >  2:132293/1‑150 (MQ=255)

                                                                ggCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAt                                                                                  <  1:3454553/150‑1 (MQ=255)

                                                                 gCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAtt                                                                                 <  2:1070451/150‑1 (MQ=255)

                                                                   aaCCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGa                                                                               <  2:1311836/150‑1 (MQ=255)

                                                                      cGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGaaaa                                                                            <  2:2596797/150‑1 (MQ=255)

                                                                      cGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGaaaa                                                                            >  1:78832/1‑150 (MQ=255)

                                                                       gCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGaaaaa                                                                           <  1:254688/150‑1 (MQ=255)

                                                                          aGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTg                                                                        <  2:604463/150‑1 (MQ=255)

                                                                            tttGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTc                                                                      >  2:2328336/1‑150 (MQ=255)

                                                                             ttGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCt                                                                     <  2:1951885/150‑1 (MQ=255)

                                                                               gCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGa                                                                   <  2:3165858/150‑1 (MQ=255)

                                                                               gCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGa                                                                   <  1:3371080/150‑1 (MQ=255)

                                                                                 tGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAAt                                                                 >  2:115056/1‑150 (MQ=255)

                                                                                  ggCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATg                                                                <  2:3552896/150‑1 (MQ=255)

                                                                                     aaGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCa                                                             <  2:2499045/150‑1 (MQ=255)

                                                                                      aGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGTTTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAg                                                            <  1:1366553/150‑1 (MQ=255)

                                                                                      aGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAg                                                            <  2:355815/150‑1 (MQ=255)

                                                                                          gcgTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAg                                                        <  1:553123/150‑1 (MQ=255)

                                                                                            gTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAgtg                                                      >  2:2082512/1‑150 (MQ=255)

                                                                                             ttGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAgtgt                                                     >  1:540636/1‑150 (MQ=255)

                                                                                               gACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGc                                                   >  1:1844841/1‑150 (MQ=255)

                                                                                                  gCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTc                                                <  2:237186/150‑1 (MQ=255)

                                                                                                  gCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTc                                                <  2:2435016/150‑1 (MQ=255)

                                                                                                  gCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTc                                                <  1:98122/150‑1 (MQ=255)

                                                                                                  gCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTc                                                <  2:1863649/150‑1 (MQ=255)

                                                                                                    ccAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGt                                              >  2:1420975/1‑150 (MQ=255)

                                                                                                    ccAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGt                                              >  2:842980/1‑150 (MQ=255)

                                                                                                     cAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTg                                             <  1:1473282/150‑1 (MQ=255)

                                                                                                     cAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTg                                             <  2:301828/150‑1 (MQ=255)

                                                                                                      aGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGc                                            <  2:3754780/150‑1 (MQ=255)

                                                                                                      aGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGc                                            <  2:2845973/150‑1 (MQ=255)

                                                                                                       gggCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGcc                                           <  2:1486668/150‑1 (MQ=255)

                                                                                                       gggCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGcc                                           >  1:2295817/1‑150 (MQ=255)

                                                                                                        ggCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCcg                                          <  2:1205165/150‑1 (MQ=255)

                                                                                                                ggtgATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAg                                  <  2:476411/150‑1 (MQ=255)

                                                                                                                   gATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCa                               <  2:2140680/150‑1 (MQ=255)

                                                                                                                       gATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATg                           <  2:1583734/150‑1 (MQ=255)

                                                                                                                           aCGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAgg                       <  1:340184/150‑1 (MQ=255)

                                                                                                                               tGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGAtct                   <  1:3520909/150‑1 (MQ=255)

                                                                                                                                gATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGAtctc                  <  1:3135128/150‑1 (MQ=255)

                                                                                                                                  tttCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCAc                <  1:3205016/150‑1 (MQ=255)

                                                                                                                                     cAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCa             <  1:2035250/150‑1 (MQ=255)

                                                                                                                                     cAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCa             <  1:1249851/150‑1 (MQ=255)

                                                                                                                                      aGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAg            >  1:3132944/1‑150 (MQ=255)

                                                                                                                                         gTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCg         >  1:2841574/1‑150 (MQ=255)

                                                                                                                                           cACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgca       >  2:194334/1‑150 (MQ=255)

                                                                                                                                             ccTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCgcagc     >  2:920868/1‑150 (MQ=255)

                                                                                                                                               ttGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCat   <  1:419025/150‑1 (MQ=255)

TGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATA  >  CP009273/3231202‑3231495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTA  >  CP009273/3231200‑3231498

TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCC                                                                                                                                                       <  SRR19973061.3531075/150‑1 (MQ=60)

TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCC                                                                                                                                                       <  SRR19973061.1320167/150‑1 (MQ=60)

   GGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGATGACGCCCAGGGCGAGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCA                                                                                                                                                    >  SRR19973061.877885/1‑150 (MQ=60)

     ATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACT                                                                                                                                                  >  SRR19973061.2392071/1‑150 (MQ=60)

     ATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACT                                                                                                                                                  >  SRR19973061.3564149/1‑150 (MQ=60)

            TTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGT                                                                                                                                           <  SRR19973061.1242716/150‑1 (MQ=60)

            TTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGT                                                                                                                                           <  SRR19973061.1266778/150‑1 (MQ=60)

            TTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGT                                                                                                                                           <  SRR19973061.3039949/150‑1 (MQ=60)

               TTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAG                                                                                                                                        >  SRR19973061.2853262/1‑150 (MQ=60)

               TTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAG                                                                                                                                        >  SRR19973061.2552499/1‑150 (MQ=60)

               TTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAG                                                                                                                                        >  SRR19973061.3086905/1‑150 (MQ=60)

                TACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGT                                                                                                                                       <  SRR19973061.3587471/150‑1 (MQ=60)

                    GTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATT                                                                                                                                   <  SRR19973061.420892/150‑1 (MQ=60)

                    GTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATT                                                                                                                                   <  SRR19973061.2443238/150‑1 (MQ=60)

                    GTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATT                                                                                                                                   >  SRR19973061.3173980/1‑150 (MQ=60)

                     TCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTC                                                                                                                                  >  SRR19973061.2505407/1‑150 (MQ=60)

                     TCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTC                                                                                                                                  >  SRR19973061.302627/1‑150 (MQ=60)

                      CTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCT                                                                                                                                 <  SRR19973061.3521921/150‑1 (MQ=60)

                      CTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCT                                                                                                                                 <  SRR19973061.2383992/150‑1 (MQ=60)

                           GTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCT                                                                                                                            >  SRR19973061.3135651/1‑150 (MQ=60)

                           GTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCT                                                                                                                            <  SRR19973061.2949868/150‑1 (MQ=60)

                                 GGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAG                                                                                                                      >  SRR19973061.963272/1‑150 (MQ=60)

                                 GGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAG                                                                                                                      >  SRR19973061.963274/1‑150 (MQ=60)

                                     GCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAG                                                                                                                  >  SRR19973061.1693822/1‑150 (MQ=60)

                                     GCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAG                                                                                                                  >  SRR19973061.1762356/1‑150 (MQ=60)

                                      CGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCGGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGA                                                                                                                 >  SRR19973061.2676897/1‑150 (MQ=60)

                                      CGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGA                                                                                                                 >  SRR19973061.821764/1‑150 (MQ=60)

                                      CGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGA                                                                                                                 >  SRR19973061.2146057/1‑150 (MQ=60)

                                      CGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGA                                                                                                                 >  SRR19973061.233986/1‑150 (MQ=60)

                                      CGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGA                                                                                                                 >  SRR19973061.2732105/1‑150 (MQ=60)

                                        TTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATT                                                                                                               <  SRR19973061.1132171/150‑1 (MQ=60)

                                             TGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGG                                                                                                          <  SRR19973061.3539844/150‑1 (MQ=60)

                                             TGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGG                                                                                                          <  SRR19973061.3540007/150‑1 (MQ=60)

                                             TGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGG                                                                                                          >  SRR19973061.1809721/1‑150 (MQ=60)

                                                TGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGA                                                                                                       >  SRR19973061.343316/1‑150 (MQ=60)

                                                 GAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAA                                                                                                      <  SRR19973061.2126069/150‑1 (MQ=60)

                                                 GAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAA                                                                                                      <  SRR19973061.3116350/150‑1 (MQ=60)

                                                      GAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGTGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGG                                                                                                 <  SRR19973061.3334069/150‑1 (MQ=60)

                                                      GAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGG                                                                                                 >  SRR19973061.550435/1‑150 (MQ=60)

                                                      GAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGG                                                                                                 >  SRR19973061.550467/1‑150 (MQ=60)

                                                      GAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGG                                                                                                 <  SRR19973061.1430545/150‑1 (MQ=60)

                                                      GAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGACGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGG                                                                                                 <  SRR19973061.2123897/150‑1 (MQ=60)

                                                       AAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGA                                                                                                >  SRR19973061.1838324/1‑150 (MQ=60)

                                                        AAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGAT                                                                                               >  SRR19973061.3281779/1‑150 (MQ=60)

                                                        AAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGAT                                                                                               >  SRR19973061.3282194/1‑150 (MQ=60)

                                                        AAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGAT                                                                                               <  SRR19973061.939332/150‑1 (MQ=60)

                                                         AAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATC                                                                                              <  SRR19973061.3182541/150‑1 (MQ=60)

                                                           GCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGC                                                                                            >  SRR19973061.1502542/1‑150 (MQ=60)

                                                            CGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCT                                                                                           <  SRR19973061.1443257/150‑1 (MQ=60)

                                                              TGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGG                                                                                         >  SRR19973061.1055363/1‑150 (MQ=60)

                                                               GCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGT                                                                                        >  SRR19973061.3468645/1‑150 (MQ=60)

                                                                CAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTG                                                                                       >  SRR19973061.3472836/1‑150 (MQ=60)

                                                                  GGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAT                                                                                     <  SRR19973061.106213/150‑1 (MQ=60)

                                                                  GGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAT                                                                                     <  SRR19973061.3463416/150‑1 (MQ=60)

                                                                  GGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAT                                                                                     <  SRR19973061.3463496/150‑1 (MQ=60)

                                                                  GGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGAT                                                                                     <  SRR19973061.3463475/150‑1 (MQ=60)

                                                                    CAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTG                                                                                   <  SRR19973061.1832726/150‑1 (MQ=60)

                                                                        CGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAA                                                                               >  SRR19973061.79117/1‑150 (MQ=60)

                                                                         GCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAA                                                                              <  SRR19973061.255392/150‑1 (MQ=60)

                                                                                TGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTG                                                                       <  SRR19973061.2675086/150‑1 (MQ=60)

                                                                                 GCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGA                                                                      <  SRR19973061.3379746/150‑1 (MQ=60)

                                                                                       AAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCA                                                                >  SRR19973061.1783746/1‑150 (MQ=60)

                                                                                        AGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGTTTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAG                                                               <  SRR19973061.1370007/150‑1 (MQ=60)

                                                                                           GGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAA                                                            >  SRR19973061.646996/1‑150 (MQ=60)

                                                                                            GCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAG                                                           <  SRR19973061.554578/150‑1 (MQ=60)

                                                                                              GTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCACACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTG                                                         <  SRR19973061.3389979/150‑1 (MQ=60)

                                                                                               TTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGT                                                        >  SRR19973061.542058/1‑150 (MQ=60)

                                                                                                 GACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGC                                                      <  SRR19973061.2980921/150‑1 (MQ=60)

                                                                                                 GACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGC                                                      >  SRR19973061.1849456/1‑150 (MQ=60)

                                                                                                    GCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTC                                                   <  SRR19973061.98446/150‑1 (MQ=60)

                                                                                                    GCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTC                                                   <  SRR19973061.98693/150‑1 (MQ=60)

                                                                                                     CCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCG                                                  >  SRR19973061.2297463/1‑150 (MQ=60)

                                                                                                       CAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTG                                                >  SRR19973061.328614/1‑150 (MQ=60)

                                                                                                       CAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTG                                                <  SRR19973061.1476983/150‑1 (MQ=60)

                                                                                                        AGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGC                                               >  SRR19973061.1955695/1‑150 (MQ=60)

                                                                                                         GGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCC                                              >  SRR19973061.2301628/1‑150 (MQ=60)

                                                                                                            CGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCG                                           <  SRR19973061.3538443/150‑1 (MQ=60)

                                                                                                                GTGGTGATGGATTAAGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGC                                       <  SRR19973061.2631237/150‑1 (MQ=60)

                                                                                                                 TGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCA                                      >  SRR19973061.136908/1‑150 (MQ=60)

                                                                                                                 TGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCA                                      >  SRR19973061.928366/1‑150 (MQ=60)

                                                                                                                 TGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCA                                      >  SRR19973061.137531/1‑150 (MQ=60)

                                                                                                                 TGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCA                                      <  SRR19973061.172317/150‑1 (MQ=60)

                                                                                                                        GGATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAAT                               <  SRR19973061.640954/150‑1 (MQ=60)

                                                                                                                         GATTACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATG                              <  SRR19973061.550276/150‑1 (MQ=60)

                                                                                                                             ACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGG                          <  SRR19973061.341086/150‑1 (MQ=60)

                                                                                                                             ACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGG                          >  SRR19973061.1833173/1‑150 (MQ=60)

                                                                                                                             ACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGG                          <  SRR19973061.3036447/150‑1 (MQ=60)

                                                                                                                             ACGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGG                          <  SRR19973061.3068493/150‑1 (MQ=60)

                                                                                                                              CGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGA                         <  SRR19973061.158063/150‑1 (MQ=60)

                                                                                                                              CGGTGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGA                         <  SRR19973061.2830569/150‑1 (MQ=60)

                                                                                                                                 TGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCT                      <  SRR19973061.3529927/150‑1 (MQ=60)

                                                                                                                                 TGATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCT                      <  SRR19973061.3529707/150‑1 (MQ=60)

                                                                                                                                  GATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTC                     <  SRR19973061.3172593/150‑1 (MQ=60)

                                                                                                                                  GATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTC                     <  SRR19973061.3143187/150‑1 (MQ=60)

                                                                                                                                  GATTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTC                     <  SRR19973061.3143201/150‑1 (MQ=60)

                                                                                                                                    TTTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCAC                   <  SRR19973061.3213251/150‑1 (MQ=60)

                                                                                                                                     TTCAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACC                  <  SRR19973061.1846008/150‑1 (MQ=60)

                                                                                                                                       CAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCA                <  SRR19973061.2040360/150‑1 (MQ=60)

                                                                                                                                       CAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCA                <  SRR19973061.3157041/150‑1 (MQ=60)

                                                                                                                                       CAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCA                <  SRR19973061.1252946/150‑1 (MQ=60)

                                                                                                                                       CAGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCA                <  SRR19973061.1252947/150‑1 (MQ=60)

                                                                                                                                        AGCGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAG               >  SRR19973061.3141012/1‑150 (MQ=60)

                                                                                                                                          CGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCC             <  SRR19973061.2423778/150‑1 (MQ=60)

                                                                                                                                          CGTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCC             >  SRR19973061.2686626/1‑150 (MQ=60)

                                                                                                                                           GTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCG            >  SRR19973061.2487519/1‑150 (MQ=60)

                                                                                                                                           GTCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCG            >  SRR19973061.2848852/1‑150 (MQ=60)

                                                                                                                                            TCACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGC           <  SRR19973061.51486/150‑1 (MQ=60)

                                                                                                                                             CACCTTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGTCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCA          >  SRR19973061.1237746/1‑150 (MQ=60)

                                                                                                                                                 TTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCAT      >  SRR19973061.293391/1‑150 (MQ=60)

                                                                                                                                                 TTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCAT      <  SRR19973061.3554675/150‑1 (MQ=60)

                                                                                                                                                 TTGCCGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCAT      <  SRR19973061.420161/150‑1 (MQ=60)

                                                                                                                                                     CGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTA  <  SRR19973061.1302108/150‑1 (MQ=60)

                                                                                                                                                     CGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTA  >  SRR19973061.657775/1‑150 (MQ=60)

TATGGATATCGGTTTTTACTGTCTGGCGTCGGCGGTGGCGTTATTTGGTGAGCCGAAAAGCGTGCAGGCAACCGCCAGTTTGCTGGCAAGCGGCGTTGACGCCCAGGGCGTGGTGGTGATGGATTACGGTGATTTCAGCGTCACCTTGCAGCACTCAAAAGTCAGTGATTCTGTCCTGGCGAGCGAGATTCAGGGCGAAGCAGGATCGCTGGTGATTGAAAAACTGTCTGAATGCCAGAAAGTGTGCTTCGTGCCGCGTGGCAGCCAAATGCAGGATCTCACCCAGCCGCAGCATATTA  >  CP009273/3231200‑3231498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`