Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,400,858 C→A 92.5% intergenic (‑738/‑182) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,400,8580CA92.5% 627.8 / 42.9 227intergenic (‑738/‑182)lrhA/alaAtranscriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
Reads supporting (aligned to +/- strand):  ref base C (9/8);  new base A (101/109);  total (110/117)
Fisher's exact test for biased strand distribution p-value = 8.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 227 total aligned reads displayed.

CGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACG  >  CP009273/2400713‑2401001
                                                                                                                                                 |                                                                                                                                               
cGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCAt                                                                                                                                               <  2:1468699/148‑1 (MQ=255)
cGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAAt                                                                                                                                               >  1:2874325/1‑148 (MQ=255)
  cAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGt                                                                                                                                             <  1:520779/148‑1 (MQ=255)
   aGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTa                                                                                                                                            <  1:294752/148‑1 (MQ=255)
    gTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTaa                                                                                                                                           >  1:2920666/1‑148 (MQ=255)
     ttAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTaaa                                                                                                                                          >  2:783058/1‑148 (MQ=255)
       aaaCTCTCAGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGc                                                                                                                                        <  2:1534309/148‑1 (MQ=255)
         aCTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCat                                                                                                                                      <  2:2998067/148‑1 (MQ=255)
            ctcCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCAtata                                                                                                                                   >  2:715557/1‑148 (MQ=255)
            ctcCGTAATGAGGAAATATCATCATCGAGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCAtata                                                                                                                                   <  1:894553/148‑1 (MQ=255)
              ccGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATaca                                                                                                                                 <  1:2357155/148‑1 (MQ=255)
              ccGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATaca                                                                                                                                 >  1:2048416/1‑148 (MQ=255)
                 tAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCt                                                                                                                              <  1:2524565/148‑1 (MQ=255)
                    tGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCat                                                                                                                           <  1:1669396/148‑1 (MQ=255)
                    tGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCat                                                                                                                           <  1:305842/148‑1 (MQ=255)
                    tGAGGAAATATCATCATCGCGTTTCATGAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCat                                                                                                                           <  1:2234206/148‑1 (MQ=255)
                     gAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCatt                                                                                                                          <  1:481569/148‑1 (MQ=255)
                     gAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCatt                                                                                                                          >  2:3215296/1‑148 (MQ=255)
                     gAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCatt                                                                                                                          >  2:2927959/1‑148 (MQ=255)
                      aGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCatta                                                                                                                         >  1:1452993/1‑148 (MQ=255)
                      aGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCatta                                                                                                                         <  2:3163926/148‑1 (MQ=255)
                       ggAAATATAATCATCGAGTTTCATTAGTGAATTCTTAAATGAGTCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCattat                                                                                                                        <  2:2695198/148‑1 (MQ=255)
                        gAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCattatt                                                                                                                       >  1:907572/1‑148 (MQ=255)
                         aaaTATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTAttt                                                                                                                      <  2:66082/148‑1 (MQ=255)
                         aaaTATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTAttt                                                                                                                      >  1:1468039/1‑148 (MQ=255)
                          aaTATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTAtttt                                                                                                                     >  1:3050546/1‑148 (MQ=255)
                           atatCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTg                                                                                                                    >  1:973041/1‑148 (MQ=255)
                            tatCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGt                                                                                                                   >  1:3090970/1‑148 (MQ=255)
                             atcatcatcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTc                                                                                                                  <  2:1766800/148‑1 (MQ=255)
                                atcatcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCatt                                                                                                               <  1:1540379/148‑1 (MQ=255)
                                 tcatcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCatta                                                                                                              <  1:1372502/148‑1 (MQ=255)
                                  catcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCattat                                                                                                             >  2:2413975/1‑148 (MQ=255)
                                  catcGCGTTTAATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCattat                                                                                                             <  2:2298274/148‑1 (MQ=255)
                                     cgcgTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTaa                                                                                                          <  1:2258379/148‑1 (MQ=255)
                                       cgTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGt                                                                                                        <  1:3175134/148‑1 (MQ=255)
                                          ttCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGttat                                                                                                     <  1:2024897/148‑1 (MQ=255)
                                          ttCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGttat                                                                                                     <  1:1863670/148‑1 (MQ=255)
                                           tCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGttatt                                                                                                    >  2:2080007/1‑148 (MQ=255)
                                           tCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGttatt                                                                                                    <  2:2052848/148‑1 (MQ=255)
                                           tCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGttatt                                                                                                    <  2:1199058/148‑1 (MQ=255)
                                              ttAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACCGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAAc                                                                                                 <  1:1943046/148‑1 (MQ=255)
                                                aGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAg                                                                                               <  1:1326593/148‑1 (MQ=255)
                                                 gTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGc                                                                                              <  2:370451/148‑1 (MQ=255)
                                                    aaTTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGcaca                                                                                           >  1:1887652/1‑148 (MQ=255)
                                                    aaTTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGcaca                                                                                           >  2:1587061/1‑148 (MQ=255)
                                                        cTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCg                                                                                       <  1:854413/148‑1 (MQ=255)
                                                        cTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCg                                                                                       >  2:1648700/1‑148 (MQ=255)
                                                           aaaTGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGc                                                                                    <  2:2226150/148‑1 (MQ=255)
                                                             aTGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCt                                                                                  <  1:1184686/148‑1 (MQ=255)
                                                                 ggCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTccc                                                                              <  1:2687197/148‑1 (MQ=255)
                                                                 ggCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTccc                                                                              <  1:262628/148‑1 (MQ=255)
                                                                   cATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCct                                                                            >  1:3070341/1‑148 (MQ=255)
                                                                     ttttCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCctct                                                                          <  2:1966806/148‑1 (MQ=255)
                                                                           cacaATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCaaa                                                                    <  1:2380366/148‑1 (MQ=255)
                                                                             caATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAAt                                                                  >  1:128842/1‑148 (MQ=255)
                                                                             caATGATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAAt                                                                  <  2:1118988/148‑1 (MQ=255)
                                                                               aTTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCt                                                                >  2:2164516/1‑148 (MQ=255)
                                                                                ttATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCtt                                                               <  1:409273/148‑1 (MQ=255)
                                                                                  aTCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTAt                                                             <  1:2581802/148‑1 (MQ=255)
                                                                                  aTCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTAt                                                             <  1:2398087/148‑1 (MQ=255)
                                                                                   tCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTAtt                                                            <  1:144337/148‑1 (MQ=255)
                                                                                    cTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTc                                                           >  1:1254804/1‑148 (MQ=255)
                                                                                      tACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTg                                                         >  2:284754/1‑148 (MQ=255)
                                                                                       aCAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGc                                                        >  2:1817140/1‑148 (MQ=255)
                                                                                         acaTAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAg                                                      <  1:1506402/146‑1 (MQ=255)
                                                                                         aGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAg                                                      <  2:1102380/148‑1 (MQ=255)
                                                                                          gATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGa                                                     >  2:279474/1‑148 (MQ=255)
                                                                                            tAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGAcc                                                   <  2:864221/148‑1 (MQ=255)
                                                                                            tAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGAcc                                                   <  1:1604274/148‑1 (MQ=255)
                                                                                            tAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGAcc                                                   >  2:3289452/1‑148 (MQ=255)
                                                                                            tAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGAcc                                                   <  1:2067252/148‑1 (MQ=255)
                                                                                            tAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGAcc                                                   <  1:3126864/148‑1 (MQ=255)
                                                                                             aaaaaaaCCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCt                                                  >  2:579874/1‑148 (MQ=255)
                                                                                               aaaaaCCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTc                                                >  1:1974789/1‑148 (MQ=255)
                                                                                                aaaaCCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCa                                               >  1:3147820/1‑148 (MQ=255)
                                                                                                    ccAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAac                                           >  1:203648/1‑148 (MQ=255)
                                                                                                        aCTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCg                                       >  2:1393449/1‑148 (MQ=255)
                                                                                                          ttACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCCTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTc                                     <  2:31011/148‑1 (MQ=255)
                                                                                                           tACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTcc                                    >  2:2188361/1‑148 (MQ=255)
                                                                                                               aTTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTggg                                >  2:2652721/1‑148 (MQ=255)
                                                                                                                ttAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTgggg                               <  1:2600901/148‑1 (MQ=255)
                                                                                                                 tAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTggggg                              <  1:654976/148‑1 (MQ=255)
                                                                                                                      aTCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTAc                         >  1:1084221/1‑148 (MQ=255)
                                                                                                                       tCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACa                        <  1:1648700/148‑1 (MQ=255)
                                                                                                                       tCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACa                        >  2:133134/1‑148 (MQ=255)
                                                                                                                        cAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACAt                       >  2:1549665/1‑148 (MQ=255)
                                                                                                                         aGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACAtt                      <  1:754687/148‑1 (MQ=255)
                                                                                                                          gAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTg                     >  1:2389714/1‑148 (MQ=255)
                                                                                                                          gAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTg                     >  2:3009236/1‑148 (MQ=255)
                                                                                                                           aaCAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGt                    >  1:915035/1‑148 (MQ=255)
                                                                                                                            aCAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGtt                   <  2:167280/148‑1 (MQ=255)
                                                                                                                             cAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTc                  >  2:3163470/1‑148 (MQ=255)
                                                                                                                              aaTTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCt                 >  1:746530/1‑148 (MQ=255)
                                                                                                                               aTTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTa                <  1:2414310/148‑1 (MQ=255)
                                                                                                                               aTTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTa                >  1:1736535/1‑148 (MQ=255)
                                                                                                                               aTTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTa                >  2:2397607/1‑148 (MQ=255)
                                                                                                                                 tCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAg              >  2:1210605/1‑148 (MQ=255)
                                                                                                                                           aCATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCa    >  2:1912642/1‑148 (MQ=255)
                                                                                                                                           aCATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCa    >  2:2566515/1‑148 (MQ=255)
                                                                                                                                            cATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCAc   >  2:2753596/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACG  >  CP009273/2400713‑2401001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGC  >  CP009273/2400711‑2401003
                                                                                                                                                    |                                                                                                                                                 
CACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAAT                                                                                                                                                 >  SRR19973070.2892946/1‑150 (MQ=60)
   GCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTA                                                                                                                                              <  SRR19973070.2518341/150‑1 (MQ=60)
    CAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAA                                                                                                                                             >  SRR19973070.2939523/1‑150 (MQ=60)
    CAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAA                                                                                                                                             <  SRR19973070.524322/150‑1 (MQ=60)
     AGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAA                                                                                                                                            <  SRR19973070.296786/150‑1 (MQ=60)
       TTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAG                                                                                                                                           <  SRR19973070.1805435/150‑1 (MQ=60)
            CTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATA                                                                                                                                     >  SRR19973070.2231975/1‑150 (MQ=60)
              CTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACA                                                                                                                                   <  SRR19973070.900888/150‑1 (MQ=60)
              CTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACA                                                                                                                                   >  SRR19973070.2062274/1‑150 (MQ=60)
              CTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACA                                                                                                                                   >  SRR19973070.2062268/1‑150 (MQ=60)
              CTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTAACTATATAACATTGAATGTAAAGCATATACA                                                                                                                                   >  SRR19973070.2269438/1‑150 (MQ=60)
              CTCCGTAATGAGGAAATATCATCATCGAGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACA                                                                                                                                   <  SRR19973070.900882/150‑1 (MQ=60)
                CCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACC                                                                                                                                 <  SRR19973070.2372769/150‑1 (MQ=60)
                CCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACC                                                                                                                                 >  SRR19973070.31420/1‑150 (MQ=60)
                CCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACC                                                                                                                                 >  SRR19973070.1497678/1‑150 (MQ=60)
                   TAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCA                                                                                                                              >  SRR19973070.671291/1‑150 (MQ=60)
                   TAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCA                                                                                                                              <  SRR19973070.2541033/150‑1 (MQ=60)
                    AATGAGGACATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCAT                                                                                                                             >  SRR19973070.2065847/1‑150 (MQ=60)
                      TGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTA                                                                                                                           >  SRR19973070.1463108/1‑150 (MQ=60)
                      TGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTA                                                                                                                           <  SRR19973070.308007/150‑1 (MQ=60)
                      TGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTA                                                                                                                           <  SRR19973070.1680856/150‑1 (MQ=60)
                      TGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTA                                                                                                                           <  SRR19973070.1326650/150‑1 (MQ=60)
                      TGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTA                                                                                                                           <  SRR19973070.1326623/150‑1 (MQ=60)
                      TGAGGAAATATCATCATCGCGTTTCATGAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTA                                                                                                                           <  SRR19973070.2248936/150‑1 (MQ=60)
                       GAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTAT                                                                                                                          <  SRR19973070.484966/150‑1 (MQ=60)
                        AGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATT                                                                                                                         >  SRR19973070.914013/1‑150 (MQ=60)
                        AGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATT                                                                                                                         >  SRR19973070.914082/1‑150 (MQ=60)
                         GGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTT                                                                                                                        >  SRR19973070.1478279/1‑150 (MQ=60)
                         GGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTT                                                                                                                        >  SRR19973070.1478297/1‑150 (MQ=60)
                          GAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTT                                                                                                                       >  SRR19973070.3071179/1‑150 (MQ=60)
                          GAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTT                                                                                                                       >  SRR19973070.3069977/1‑150 (MQ=60)
                           AAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTG                                                                                                                      >  SRR19973070.979983/1‑150 (MQ=60)
                            AATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGT                                                                                                                     >  SRR19973070.3109917/1‑150 (MQ=60)
                            AATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGT                                                                                                                     >  SRR19973070.3110862/1‑150 (MQ=60)
                            AATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGT                                                                                                                     <  SRR19973070.970387/150‑1 (MQ=60)
                               ATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCAT                                                                                                                  >  SRR19973070.2555861/1‑150 (MQ=60)
                                 CATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTA                                                                                                                <  SRR19973070.2376645/150‑1 (MQ=60)
                                  ATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTAT                                                                                                               <  SRR19973070.1551204/150‑1 (MQ=60)
                                   TCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATT                                                                                                              <  SRR19973070.1381833/150‑1 (MQ=60)
                                       CGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGT                                                                                                          <  SRR19973070.2273356/150‑1 (MQ=60)
                                        GCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTT                                                                                                         <  SRR19973070.1410763/150‑1 (MQ=60)
                                         CGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTA                                                                                                        <  SRR19973070.3195890/150‑1 (MQ=60)
                                         CGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTA                                                                                                        <  SRR19973070.3195684/150‑1 (MQ=60)
                                         CGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTA                                                                                                        <  SRR19973070.3195673/150‑1 (MQ=60)
                                            TTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTA                                                                                                     <  SRR19973070.2038584/150‑1 (MQ=60)
                                            TTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTA                                                                                                     <  SRR19973070.2038597/150‑1 (MQ=60)
                                            TTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTA                                                                                                     <  SRR19973070.2038622/150‑1 (MQ=60)
                                            TTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTA                                                                                                     <  SRR19973070.1876412/150‑1 (MQ=60)
                                            TTAATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCCGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTA                                                                                                     <  SRR19973070.1876435/150‑1 (MQ=60)
                                              CATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAAC                                                                                                   >  SRR19973070.2384232/1‑150 (MQ=60)
                                                TTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACCGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAG                                                                                                 <  SRR19973070.1956097/150‑1 (MQ=60)
                                                  AGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCA                                                                                               <  SRR19973070.1335752/150‑1 (MQ=60)
                                                  AGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCA                                                                                               <  SRR19973070.2992068/150‑1 (MQ=60)
                                                    TGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACA                                                                                             >  SRR19973070.1900462/1‑150 (MQ=60)
                                                        TTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCG                                                                                         <  SRR19973070.2918114/150‑1 (MQ=60)
                                                          CTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAG                                                                                       <  SRR19973070.860188/150‑1 (MQ=60)
                                                          CTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAG                                                                                       <  SRR19973070.860300/150‑1 (MQ=60)
                                                               ATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTC                                                                                  <  SRR19973070.1193012/150‑1 (MQ=60)
                                                               ATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTC                                                                                  <  SRR19973070.1193013/150‑1 (MQ=60)
                                                               ATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTC                                                                                  <  SRR19973070.1193117/150‑1 (MQ=60)
                                                                TGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCC                                                                                 <  SRR19973070.2011423/150‑1 (MQ=60)
                                                                   GGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCT                                                                              <  SRR19973070.2704615/150‑1 (MQ=60)
                                                                   GGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCT                                                                              <  SRR19973070.264436/150‑1 (MQ=60)
                                                                   GGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCT                                                                              <  SRR19973070.264377/150‑1 (MQ=60)
                                                                   GGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCT                                                                              >  SRR19973070.3090043/1‑150 (MQ=60)
                                                                             CACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAAT                                                                    >  SRR19973070.129976/1‑150 (MQ=60)
                                                                             CACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAAT                                                                    <  SRR19973070.2396095/150‑1 (MQ=60)
                                                                                AATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTT                                                                 <  SRR19973070.1595099/150‑1 (MQ=60)
                                                                                AATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTT                                                                 <  SRR19973070.441097/150‑1 (MQ=60)
                                                                                  TTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTAT                                                               <  SRR19973070.1265653/150‑1 (MQ=60)
                                                                                  TTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTAT                                                               <  SRR19973070.412265/150‑1 (MQ=60)
                                                                                    ATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTC                                                             <  SRR19973070.2413910/150‑1 (MQ=60)
                                                                                    ATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTC                                                             >  SRR19973070.1263606/1‑150 (MQ=60)
                                                                                    ATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTC                                                             <  SRR19973070.2413907/150‑1 (MQ=60)
                                                                                    ATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTC                                                             <  SRR19973070.2598678/150‑1 (MQ=60)
                                                                                    ATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTC                                                             <  SRR19973070.1902410/150‑1 (MQ=60)
                                                                                     TCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCT                                                            <  SRR19973070.145575/150‑1 (MQ=60)
                                                                                      CTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTG                                                           <  SRR19973070.2939891/150‑1 (MQ=60)
                                                                                      CTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTG                                                           <  SRR19973070.699974/150‑1 (MQ=60)
                                                                                        TACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCA                                                         <  SRR19973070.3273616/150‑1 (MQ=60)
                                                                                        TACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCA                                                         <  SRR19973070.1625051/150‑1 (MQ=60)
                                                                                          CAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGA                                                       <  SRR19973070.2628130/150‑1 (MQ=60)
                                                                                           ACAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGAC                                                      <  SRR19973070.1516941/150‑1 (MQ=60)
                                                                                             AT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCT                                                    <  SRR19973070.3053635/150‑1 (MQ=60)
                                                                                              T‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTT                                                   <  SRR19973070.2679360/150‑1 (MQ=60)
                                                                                              T‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTT                                                   <  SRR19973070.3147051/150‑1 (MQ=60)
                                                                                              T‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTT                                                   <  SRR19973070.1615468/150‑1 (MQ=60)
                                                                                              T‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTT                                                   <  SRR19973070.2081187/150‑1 (MQ=60)
                                                                                                AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTC                                                  >  SRR19973070.1988143/1‑150 (MQ=60)
                                                                                                 AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCA                                                 >  SRR19973070.3168155/1‑150 (MQ=60)
                                                                                                 AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCA                                                 >  SRR19973070.3168160/1‑150 (MQ=60)
                                                                                                   AAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAA                                               >  SRR19973070.752013/1‑150 (MQ=60)
                                                                                                     AACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAAC                                             >  SRR19973070.1631821/1‑150 (MQ=60)
                                                                                                     AACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAAC                                             >  SRR19973070.205179/1‑150 (MQ=60)
                                                                                                     AACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAAC                                             >  SRR19973070.2400657/1‑150 (MQ=60)
                                                                                                      ACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACA                                            <  SRR19973070.2359623/150‑1 (MQ=60)
                                                                                                                CAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGG                                  >  SRR19973070.3091528/1‑150 (MQ=60)
                                                                                                                 AATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGG                                 <  SRR19973070.3083333/150‑1 (MQ=60)
                                                                                                                   TTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGA                               <  SRR19973070.1962971/150‑1 (MQ=60)
                                                                                                                   TTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGA                               >  SRR19973070.881200/1‑150 (MQ=60)
                                                                                                                   TTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGA                               >  SRR19973070.881206/1‑150 (MQ=60)
                                                                                                                   TTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGA                               <  SRR19973070.2617884/150‑1 (MQ=60)
                                                                                                                    TAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAG                              <  SRR19973070.659647/150‑1 (MQ=60)
                                                                                                                     AAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGT                             <  SRR19973070.2094005/150‑1 (MQ=60)
                                                                                                                     AAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGT                             >  SRR19973070.2823878/1‑150 (MQ=60)
                                                                                                                       GAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTAC                           >  SRR19973070.1091745/1‑150 (MQ=60)
                                                                                                                          TCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATT                        <  SRR19973070.1660082/150‑1 (MQ=60)
                                                                                                                          TCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATT                        <  SRR19973070.1660138/150‑1 (MQ=60)
                                                                                                                           CAGAACAATTCACTATATAGCATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTG                       >  SRR19973070.26182/1‑150 (MQ=60)
                                                                                                                           CAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTG                       >  SRR19973070.2405493/1‑150 (MQ=60)
                                                                                                                            AGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGT                      >  SRR19973070.921546/1‑150 (MQ=60)
                                                                                                                            AGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGT                      <  SRR19973070.760014/150‑1 (MQ=60)
                                                                                                                            AGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGT                      <  SRR19973070.2429871/150‑1 (MQ=60)
                                                                                                                             GAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTT                     >  SRR19973070.1463083/1‑150 (MQ=60)
                                                                                                                              AACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTC                    >  SRR19973070.50528/1‑150 (MQ=60)
                                                                                                                               ACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCT                   >  SRR19973070.751803/1‑150 (MQ=60)
                                                                                                                               ACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCT                   <  SRR19973070.1544635/150‑1 (MQ=60)
                                                                                                                                CAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTA                  >  SRR19973070.1748569/1‑150 (MQ=60)
                                                                                                                                CAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTA                  >  SRR19973070.1748501/1‑150 (MQ=60)
                                                                                                                                CAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTA                  >  SRR19973070.2432325/1‑150 (MQ=60)
                                                                                                                                 AATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAA                 >  SRR19973070.54668/1‑150 (MQ=60)
                                                                                                                                  ATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAG                <  SRR19973070.2430207/150‑1 (MQ=60)
                                                                                                                                      ACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGA            <  SRR19973070.1833879/150‑1 (MQ=60)
                                                                                                                                            TAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCA      >  SRR19973070.1470911/1‑150 (MQ=60)
                                                                                                                                             AACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCAC     >  SRR19973070.1371441/1‑150 (MQ=60)
                                                                                                                                                ATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGC  >  SRR19973070.2078059/1‑150 (MQ=60)
                                                                                                                                                ATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCNGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGC  >  SRR19973070.1690793/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                 
CACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAT‑AAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGC  >  CP009273/2400711‑2401003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑