Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,400,858 C→A 100% intergenic (‑738/‑182) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,400,8580CA98.1% 674.1 / 2.5 210intergenic (‑738/‑182)lrhA/alaAtranscriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base A (99/107);  total (100/110)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 210 total aligned reads displayed.

CACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGC  >  CP009273/2400711‑2401003
                                                                                                                                                   |                                                                                                                                                 
caCGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAAt                                                                                                                                                 >  1:4834389/1‑150 (MQ=255)
     aGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTaaa                                                                                                                                            >  1:5149202/1‑150 (MQ=255)
        tAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCCCTATATAACATTGAATGTAAAGCa                                                                                                                                         >  2:4604965/1‑150 (MQ=255)
          aaCTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAGTGTAAAGCata                                                                                                                                       >  2:101130/1‑150 (MQ=255)
            ctctCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCAtata                                                                                                                                     >  1:2000208/1‑150 (MQ=255)
             tctcCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAATCCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATac                                                                                                                                    >  2:3511667/1‑150 (MQ=255)
                 cGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCt                                                                                                                                >  1:4155661/1‑150 (MQ=255)
                 cGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCt                                                                                                                                >  1:4061554/1‑150 (MQ=255)
                   tAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGTATATACACCTCa                                                                                                                              <  1:3302732/150‑1 (MQ=255)
                    aaTGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCat                                                                                                                             <  2:206666/150‑1 (MQ=255)
                        aGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCattatt                                                                                                                         <  2:4109775/150‑1 (MQ=255)
                         ggAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTAttt                                                                                                                        <  1:973888/150‑1 (MQ=255)
                              tatCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCa                                                                                                                   <  1:4756138/150‑1 (MQ=255)
                               atcatcatcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCat                                                                                                                  >  2:955544/1‑150 (MQ=255)
                                  atcatcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCattat                                                                                                               >  2:2706425/1‑150 (MQ=255)
                                    catcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCAttatta                                                                                                             <  2:372389/150‑1 (MQ=255)
                                    cagcgcgTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCAttatta                                                                                                             <  2:2577485/147‑1 (MQ=255)
                                     atcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTaa                                                                                                            <  1:4989029/150‑1 (MQ=255)
                                      tcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAATAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAg                                                                                                           <  1:2222591/150‑1 (MQ=255)
                                      tcGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAg                                                                                                           <  1:4788144/150‑1 (MQ=255)
                                       cgcgTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGt                                                                                                          >  1:4883343/1‑150 (MQ=255)
                                       cgcgTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGt                                                                                                          >  1:4881063/1‑150 (MQ=255)
                                       cgcgTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGt                                                                                                          <  1:4686789/150‑1 (MQ=255)
                                         cgTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGtta                                                                                                        >  1:338393/1‑150 (MQ=255)
                                         cgTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGtta                                                                                                        <  2:1302426/150‑1 (MQ=255)
                                         cgTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGtta                                                                                                        >  2:3043287/1‑150 (MQ=255)
                                         cgTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGtta                                                                                                        >  1:4398273/1‑150 (MQ=255)
                                                 tAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGc                                                                                                <  2:3356591/150‑1 (MQ=255)
                                                  aGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGca                                                                                               >  1:3750217/1‑150 (MQ=255)
                                                    tGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGcaca                                                                                             <  1:805024/150‑1 (MQ=255)
                                                    tGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGcaca                                                                                             >  1:2585921/1‑150 (MQ=255)
                                                     gAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACaa                                                                                            <  2:370155/150‑1 (MQ=255)
                                                      aaTTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAAt                                                                                           >  2:4494516/1‑150 (MQ=255)
                                                       aTTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACAGTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAAta                                                                                          <  2:4393874/150‑2 (MQ=255)
                                                        ttCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCg                                                                                         <  2:733524/150‑1 (MQ=255)
                                                         tCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATAGa                                                                                        <  1:1088079/150‑1 (MQ=255)
                                                         tCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGa                                                                                        <  1:2661745/150‑1 (MQ=255)
                                                          cTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAg                                                                                       <  2:4037949/150‑1 (MQ=255)
                                                           ttAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGc                                                                                      >  1:859035/1‑150 (MQ=255)
                                                            tAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGcc                                                                                     <  1:2777226/150‑1 (MQ=255)
                                                             aaaTGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCt                                                                                    >  1:1244221/1‑150 (MQ=255)
                                                               aTGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTc                                                                                  >  2:2442022/1‑150 (MQ=255)
                                                                tGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATAGAGCCTTcc                                                                                 <  1:920590/150‑1 (MQ=255)
                                                                 gAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTccc                                                                                >  2:566153/1‑150 (MQ=255)
                                                                  aGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTcccc                                                                               <  2:1818964/150‑1 (MQ=255)
                                                                  aGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTcccc                                                                               <  1:3544870/150‑1 (MQ=255)
                                                                       ttttCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTgg                                                                          <  1:3995082/150‑1 (MQ=255)
                                                                        tttCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGc                                                                         <  2:3684208/150‑1 (MQ=255)
                                                                          tCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCaa                                                                       <  1:2353914/150‑1 (MQ=255)
                                                                          tCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCaa                                                                       >  2:5033398/1‑150 (MQ=255)
                                                                           cacacaATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATAGAGCCTTCCCCTCTGGCaaa                                                                      >  2:883998/1‑150 (MQ=255)
                                                                           cacacaATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCaaa                                                                      >  2:4244526/1‑150 (MQ=255)
                                                                            acacaATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCaaaa                                                                     >  1:1444133/1‑150 (MQ=255)
                                                                            acacaATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCaaaa                                                                     >  2:5058082/1‑150 (MQ=255)
                                                                            acacaATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCaaaa                                                                     >  1:4078829/1‑150 (MQ=255)
                                                                             cacaATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAAt                                                                    <  1:1280986/150‑1 (MQ=255)
                                                                                aaTTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCtt                                                                 <  2:3127710/150‑1 (MQ=255)
                                                                                 aTTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTa                                                                >  1:1006130/1‑150 (MQ=255)
                                                                                   tATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTAtt                                                              >  1:246162/1‑150 (MQ=255)
                                                                                     tCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCt                                                            <  1:411658/150‑1 (MQ=255)
                                                                                      cTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTg                                                           <  2:175812/150‑1 (MQ=255)
                                                                                      cTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTg                                                           <  1:3040234/150‑1 (MQ=255)
                                                                                       ttACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGc                                                          <  2:5108284/150‑1 (MQ=255)
                                                                                        tACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCa                                                         >  2:292607/1‑150 (MQ=255)
                                                                                          cAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGa                                                       >  1:2633798/1‑150 (MQ=255)
                                                                                          cAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGa                                                       <  2:1559151/150‑1 (MQ=255)
                                                                                             aTAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCt                                                    >  1:4565856/1‑150 (MQ=255)
                                                                                             aTAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCt                                                    >  1:431635/1‑150 (MQ=255)
                                                                                              tAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCtt                                                   <  2:2011399/150‑1 (MQ=255)
                                                                                              tAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCtt                                                   >  2:2593203/1‑150 (MQ=255)
                                                                                               aaaaaaaCCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATCATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTc                                                  >  2:2741301/1‑150 (MQ=255)
                                                                                                    aaCCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAac                                             >  2:814584/1‑150 (MQ=255)
                                                                                                            ttACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCt                                     <  2:638523/150‑1 (MQ=255)
                                                                                                             tACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTg                                    <  2:1593052/150‑1 (MQ=255)
                                                                                                                   tAAGAATCAGAATAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTACCCCTCTGGCAAAAACTAATTCTGCACACATTCAAAACACCGTCCTGGGTGAg                              >  1:4342214/1‑150 (MQ=255)
                                                                                                                   tAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAg                              >  1:3210876/1‑150 (MQ=255)
                                                                                                                   tAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAg                              >  1:3210797/1‑150 (MQ=255)
                                                                                                                     aGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTa                            >  1:4836989/1‑150 (MQ=255)
                                                                                                                     aGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTa                            <  2:5149202/150‑1 (MQ=255)
                                                                                                                      gAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTAc                           <  1:4212523/150‑1 (MQ=255)
                                                                                                                       aaTCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACa                          <  2:166104/150‑1 (MQ=255)
                                                                                                                         tCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACAtt                        <  1:2847761/150‑1 (MQ=255)
                                                                                                                           aGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGt                      >  1:3327344/1‑150 (MQ=255)
                                                                                                                           aGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGt                      >  2:1975980/1‑150 (MQ=255)
                                                                                                                               cAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGTCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTa                  >  1:3759521/1‑150 (MQ=255)
                                                                                                                                   tCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCt              <  1:173909/150‑1 (MQ=255)
                                                                                                                                   tCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCt              <  1:4909796/150‑1 (MQ=255)
                                                                                                                                   tCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCt              <  1:5097851/150‑1 (MQ=255)
                                                                                                                                   tCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCt              >  2:26361/1‑150 (MQ=255)
                                                                                                                                     aCTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGa            <  1:2337693/150‑1 (MQ=255)
                                                                                                                                      cTATATAACATTGAATGTAAAGCATATACACCTCATTATTTCGTCATTATTAAGTTATTAACAGCACAATCGAGCCGTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGAc           <  2:4426678/150‑1 (MQ=255)
                                                                                                                                         tataACATTGAATGTAAAGTATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTc        <  1:4722096/150‑1 (MQ=255)
                                                                                                                                          ataACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTcc       <  2:2152734/150‑1 (MQ=255)
                                                                                                                                          ataACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTcc       <  2:4834389/150‑1 (MQ=255)
                                                                                                                                           taACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCa      >  1:3156629/1‑150 (MQ=255)
                                                                                                                                           taACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCa      >  2:638149/1‑150 (MQ=255)
                                                                                                                                           taACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCa      >  2:3229402/1‑150 (MQ=255)
                                                                                                                                            aaCATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCAc     <  2:3522055/150‑1 (MQ=255)
                                                                                                                                              cATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACgg   <  1:671320/150‑1 (MQ=255)
                                                                                                                                               aTTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGc  <  2:4980741/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGC  >  CP009273/2400711‑2401003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGG  >  CP009273/2400709‑2401006
                                                                                                                                                     |                                                                                                                                                    
ATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGA                                                                                                                                                      >  SRR19973063.166614/1‑150 (MQ=60)
 TCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAA                                                                                                                                                     >  SRR19973063.4168336/1‑150 (MQ=60)
  CACGCAGTTAAACTCTCCGTAGTGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAAT                                                                                                                                                    >  SRR19973063.640401/1‑150 (MQ=60)
  CACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAAT                                                                                                                                                    >  SRR19973063.489534/1‑150 (MQ=60)
  CACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAAT                                                                                                                                                    >  SRR19973063.4849904/1‑150 (MQ=60)
  CACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAAT                                                                                                                                                    >  SRR19973063.1847750/1‑150 (MQ=60)
       AGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAA                                                                                                                                               >  SRR19973063.5165773/1‑150 (MQ=60)
         TTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGC                                                                                                                                             >  SRR19973063.2464904/1‑150 (MQ=60)
           AAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCAT                                                                                                                                           >  SRR19973063.4730932/1‑150 (MQ=60)
              CTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATA                                                                                                                                        >  SRR19973063.2006455/1‑150 (MQ=60)
                   CGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCT                                                                                                                                   >  SRR19973063.4074572/1‑150 (MQ=60)
                   CGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCT                                                                                                                                   >  SRR19973063.2326643/1‑150 (MQ=60)
                   CGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCT                                                                                                                                   >  SRR19973063.4168951/1‑150 (MQ=60)
                     TAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGTATATACACCTCA                                                                                                                                 <  SRR19973063.3313302/150‑1 (MQ=60)
                     TAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACTTCA                                                                                                                                 <  SRR19973063.4921044/150‑1 (MQ=60)
                          AGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATT                                                                                                                            <  SRR19973063.4013950/150‑1 (MQ=60)
                           GGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTT                                                                                                                           <  SRR19973063.976826/150‑1 (MQ=60)
                           GGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTT                                                                                                                           >  SRR19973063.2350988/1‑150 (MQ=60)
                              AATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGT                                                                                                                        <  SRR19973063.3289161/150‑1 (MQ=60)
                                TATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCA                                                                                                                      <  SRR19973063.4771439/150‑1 (MQ=60)
                                TATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCA                                                                                                                      <  SRR19973063.4771303/150‑1 (MQ=60)
                                   CATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTA                                                                                                                   <  SRR19973063.4121024/150‑1 (MQ=60)
                                   CATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTA                                                                                                                   <  SRR19973063.3954819/150‑1 (MQ=60)
                                     TCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATT                                                                                                                 >  SRR19973063.1410871/1‑150 (MQ=60)
                                       ATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAA                                                                                                               <  SRR19973063.5005148/150‑1 (MQ=60)
                                        TCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAATAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAG                                                                                                              <  SRR19973063.2229620/150‑1 (MQ=60)
                                        TCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAG                                                                                                              <  SRR19973063.4803441/150‑1 (MQ=60)
                                        TCGAGTTTCATTAGTGAATTCTTACATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAG                                                                                                              <  SRR19973063.4803482/150‑1 (MQ=60)
                                         CGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGT                                                                                                             >  SRR19973063.4896804/1‑150 (MQ=60)
                                         CGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGT                                                                                                             >  SRR19973063.4899094/1‑150 (MQ=60)
                                         CGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGT                                                                                                             <  SRR19973063.4701732/150‑1 (MQ=60)
                                           CGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTA                                                                                                           >  SRR19973063.339372/1‑150 (MQ=60)
                                           CGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTA                                                                                                           >  SRR19973063.339383/1‑150 (MQ=60)
                                           CGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTA                                                                                                           >  SRR19973063.4412366/1‑150 (MQ=60)
                                                    AGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCA                                                                                                  >  SRR19973063.3762199/1‑150 (MQ=60)
                                                     GTGAATTCTTATATGTGGAATTTTCACACTATTATCTTACAGATATAAAAACAAGACTTACAATTAAGAATCAGAACAATTCACTATATTAAATTGAATGTAAAGTATATACACCTCATTATTTTGTCATTATTAAGTTATTAACATCAC                                                                                                 <  SRR19973063.832512/150‑1 (MQ=60)
                                                      TGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACA                                                                                                <  SRR19973063.807222/150‑1 (MQ=60)
                                                      TGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACA                                                                                                <  SRR19973063.807346/150‑1 (MQ=60)
                                                      TGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACA                                                                                                >  SRR19973063.2594143/1‑150 (MQ=60)
                                                          TTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATcgcgcatttcgtgcagcggaaatcgttttgact                                                                                            >  SRR19973063.2349783/1‑117 (MQ=60)
                                                           TCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATAGA                                                                                           <  SRR19973063.1091387/150‑1 (MQ=60)
                                                           TCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGA                                                                                           <  SRR19973063.2670338/150‑1 (MQ=60)
                                                           TCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGA                                                                                           <  SRR19973063.2670276/150‑1 (MQ=60)
                                                             TTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGC                                                                                         >  SRR19973063.861508/1‑150 (MQ=60)
                                                              TAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCC                                                                                        >  SRR19973063.1621007/1‑150 (MQ=60)
                                                              TAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCC                                                                                        <  SRR19973063.2786172/150‑1 (MQ=60)
                                                               AAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCT                                                                                       >  SRR19973063.1247935/1‑150 (MQ=60)
                                                               AAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCT                                                                                       <  SRR19973063.82372/150‑1 (MQ=60)
                                                               AAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATAATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCT                                                                                       >  SRR19973063.2960445/1‑150 (MQ=60)
                                                                  TGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATAGAGCCTTCC                                                                                    <  SRR19973063.923308/150‑1 (MQ=60)
                                                                    AGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCC                                                                                  <  SRR19973063.3556229/150‑1 (MQ=60)
                                                                       CATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCT                                                                               >  SRR19973063.4171485/1‑150 (MQ=60)
                                                                         TTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGG                                                                             <  SRR19973063.4007893/150‑1 (MQ=60)
                                                                            TCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAA                                                                          >  SRR19973063.3074704/1‑150 (MQ=60)
                                                                            TCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAA                                                                          <  SRR19973063.2361448/150‑1 (MQ=60)
                                                                            TCACACAATTATCTTACAGATAAAAAAACCAGAATTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACTACTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAA                                                                          <  SRR19973063.2593663/150‑1 (MQ=60)
                                                                              ACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAA                                                                        >  SRR19973063.1448541/1‑150 (MQ=60)
                                                                              ACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAA                                                                        >  SRR19973063.4091623/1‑150 (MQ=60)
                                                                              ACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAA                                                                        >  SRR19973063.4091893/1‑150 (MQ=60)
                                                                               CACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAAT                                                                       <  SRR19973063.1284806/150‑1 (MQ=60)
                                                                                ACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATC                                                                      >  SRR19973063.4626536/1‑150 (MQ=60)
                                                                                  AATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTT                                                                    <  SRR19973063.5013405/150‑1 (MQ=60)
                                                                                   ATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTA                                                                   >  SRR19973063.1009160/1‑150 (MQ=60)
                                                                                     TATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATT                                                                 >  SRR19973063.246911/1‑150 (MQ=60)
                                                                                       TCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCT                                                               <  SRR19973063.412815/150‑1 (MQ=60)
                                                                                        CTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTG                                                              <  SRR19973063.3049947/150‑1 (MQ=60)
                                                                                            CAGATACAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGA                                                          >  SRR19973063.2649779/1‑150 (MQ=60)
                                                                                            CAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGA                                                          >  SRR19973063.2642203/1‑150 (MQ=60)
                                                                                            CAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGA                                                          <  SRR19973063.4266424/150‑1 (MQ=60)
                                                                                              GATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACC                                                        <  SRR19973063.4809088/150‑1 (MQ=60)
                                                                                               ATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCT                                                       >  SRR19973063.432834/1‑150 (MQ=60)
                                                                                               ATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCT                                                       <  SRR19973063.4863993/150‑1 (MQ=60)
                                                                                               ATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCT                                                       >  SRR19973063.4580472/1‑150 (MQ=60)
                                                                                               ATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCT                                                       <  SRR19973063.4390641/150‑1 (MQ=60)
                                                                                                TAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTT                                                      >  SRR19973063.4161676/1‑150 (MQ=60)
                                                                                                  AAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATAAACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTACA                                                    >  SRR19973063.2829241/1‑150 (MQ=60)
                                                                                                        CCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACAC                                              >  SRR19973063.3250580/1‑150 (MQ=60)
                                                                                                         CAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACC                                             >  SRR19973063.242567/1‑150 (MQ=60)
                                                                                                          AGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCG                                            <  SRR19973063.917338/150‑1 (MQ=60)
                                                                                                          AGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCG                                            >  SRR19973063.3387127/1‑150 (MQ=60)
                                                                                                                    TTAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGA                                  >  SRR19973063.5154032/1‑150 (MQ=60)
                                                                                                                     TAAGAATCAGAATAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAG                                 >  SRR19973063.2177427/1‑150 (MQ=60)
                                                                                                                     TAAGAATCAGAATAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTACCCCTCTGGCAAAAACTAATTCTGCACACATTCAAAACACCGTCCTGGGTGAG                                 >  SRR19973063.4356066/1‑150 (MQ=60)
                                                                                                                     TAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAG                                 >  SRR19973063.3221120/1‑150 (MQ=60)
                                                                                                                     TAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAG                                 >  SRR19973063.3221198/1‑150 (MQ=60)
                                                                                                                     TAAGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAG                                 >  SRR19973063.3221199/1‑150 (MQ=60)
                                                                                                                       AGAATCAGAACAATTCACTATATAACATTGAGTGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTA                               <  SRR19973063.4998488/150‑1 (MQ=60)
                                                                                                                       AGAATCAGAACAATTCACTATATAACATTGAGTGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTA                               <  SRR19973063.101477/150‑1 (MQ=60)
                                                                                                                       AGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAATATCTTATTCTGCAGACCGTCAAATCACCGTCCTGGGGGAGGA                               >  SRR19973063.4852439/1‑150 (MQ=60)
                                                                                                                       AGAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTA                               >  SRR19973063.4852526/1‑150 (MQ=60)
                                                                                                                        GAATCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTAC                              <  SRR19973063.4225960/150‑1 (MQ=60)
                                                                                                                           TCAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATT                           <  SRR19973063.2856895/150‑1 (MQ=60)
                                                                                                                            CAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCCAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTG                          >  SRR19973063.4422007/1‑150 (MQ=60)
                                                                                                                            CAGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTG                          >  SRR19973063.3615843/1‑150 (MQ=60)
                                                                                                                             AGAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGT                         >  SRR19973063.3337960/1‑150 (MQ=60)
                                                                                                                              GAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTT                        >  SRR19973063.952667/1‑150 (MQ=60)
                                                                                                                              GAACAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTT                        >  SRR19973063.3320978/1‑150 (MQ=60)
                                                                                                                                 CAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGTCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTA                     >  SRR19973063.3770251/1‑150 (MQ=60)
                                                                                                                                 CAATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGTCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTA                     >  SRR19973063.3771524/1‑150 (MQ=60)
                                                                                                                                  AATTCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAA                    >  SRR19973063.4344315/1‑150 (MQ=60)
                                                                                                                                     TCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCT                 <  SRR19973063.4925649/150‑1 (MQ=60)
                                                                                                                                     TCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCT                 <  SRR19973063.5114255/150‑1 (MQ=60)
                                                                                                                                     TCACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCT                 <  SRR19973063.174448/150‑1 (MQ=60)
                                                                                                                                       ACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGA               <  SRR19973063.1186049/150‑1 (MQ=60)
                                                                                                                                       ACTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGA               <  SRR19973063.2345169/150‑1 (MQ=60)
                                                                                                                                        CTATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGAC              <  SRR19973063.2600656/150‑1 (MQ=60)
                                                                                                                                         TATATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACT             <  SRR19973063.3221065/150‑1 (MQ=60)
                                                                                                                                           TATAACATTGAATGTAAAGTATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTC           <  SRR19973063.4737151/150‑1 (MQ=60)
                                                                                                                                           TATAACATTGAATGTAAAGTATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTC           <  SRR19973063.4737150/150‑1 (MQ=60)
                                                                                                                                           TATAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTC           >  SRR19973063.1255116/1‑150 (MQ=60)
                                                                                                                                             TAACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCA         >  SRR19973063.3166789/1‑150 (MQ=60)
                                                                                                                                              AACATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCAC        <  SRR19973063.4150673/150‑1 (MQ=60)
                                                                                                                                                CATTGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGG      <  SRR19973063.673283/150‑1 (MQ=60)
                                                                                                                                                   TGAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAG   >  SRR19973063.4349396/1‑150 (MQ=60)
                                                                                                                                                   TGAATGTAAAGCATATACACCTCAATATTTTGTCATTATTAAGTAATTAACAGCACAATCGAGCCTTCCACTCTGCCAAAATCTTATTCTGCAGACCTTAAAAAAACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCAACGTCAG   >  SRR19973063.4345554/1‑150 (MQ=60)
                                                                                                                                                    GAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGG  >  SRR19973063.2568584/1‑150 (MQ=60)
                                                                                                                                                    GAATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGG  <  SRR19973063.5176268/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
ATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTACAATTAAGAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGG  >  CP009273/2400709‑2401006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑