Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,825,783 C→T 100% intergenic (‑135/‑52) norR ← / → norV anaerobic nitric oxide reductase DNA‑binding transcriptional activator/anaerobic nitric oxide reductase flavorubredoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,825,7830CT97.2% 856.1 / 9.4 250intergenic (‑135/‑52)norR/norVanaerobic nitric oxide reductase DNA‑binding transcriptional activator/anaerobic nitric oxide reductase flavorubredoxin
Reads supporting (aligned to +/- strand):  ref base C (5/2);  new base T (120/123);  total (125/125)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 250 total aligned reads displayed.

CGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGC  >  CP009273/2825636‑2825925
                                                                                                                                                   |                                                                                                                                              
cGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTg                                                                                                                                              <  1:259064/150‑1 (MQ=255)
 tgAAAAACTCATCTTTGCCTCACTGTCAATTTTACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGTGCATAATTTTATTTATATAGTAAAAACAATCAGATAAAAAACTGGCACTCAATTTGc                                                                                                                                             >  2:322691/2‑150 (MQ=255)
   aaaaaCTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCaa                                                                                                                                           >  1:1029255/1‑150 (MQ=255)
   aaaaaCTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCaa                                                                                                                                           >  2:137818/1‑150 (MQ=255)
      aaCTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTa                                                                                                                                        <  2:190479/150‑1 (MQ=255)
        cTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGTAATTTGCAATTAGc                                                                                                                                      <  1:292394/150‑1 (MQ=255)
          cATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCACTTTGCAATTAGCaa                                                                                                                                    >  2:3464349/1‑150 (MQ=255)
           aTCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAg                                                                                                                                   >  1:523789/1‑150 (MQ=255)
            tCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGa                                                                                                                                  <  1:3136986/150‑1 (MQ=255)
             cTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGAc                                                                                                                                 <  1:2742784/150‑1 (MQ=255)
             cTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGAc                                                                                                                                 <  1:2742765/150‑1 (MQ=255)
             cTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATGTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGAc                                                                                                                                 >  2:4189744/1‑150 (MQ=255)
                  ccTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCtt                                                                                                                            >  2:837735/1‑150 (MQ=255)
                  ccTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCtt                                                                                                                            <  1:70240/150‑1 (MQ=255)
                   cTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCttt                                                                                                                           >  1:951255/1‑150 (MQ=255)
                    tCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCtttt                                                                                                                          <  1:796308/150‑1 (MQ=255)
                     cACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCttttt                                                                                                                         >  1:1382837/1‑150 (MQ=255)
                       cTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAg                                                                                                                       <  1:2103193/150‑1 (MQ=255)
                          tCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAac                                                                                                                    <  2:2272395/150‑1 (MQ=255)
                          tCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAac                                                                                                                    <  1:692176/150‑1 (MQ=255)
                            aaTTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAacac                                                                                                                  <  1:623732/150‑1 (MQ=255)
                                  aCTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGaa                                                                                                            <  2:247524/150‑1 (MQ=255)
                                   cTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAAt                                                                                                           >  1:3829837/1‑150 (MQ=255)
                                   cTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAAt                                                                                                           >  2:263339/1‑150 (MQ=255)
                                     ataGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATaa                                                                                                         >  2:3252957/1‑150 (MQ=255)
                                            ttGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAgg                                                                                                  >  1:479135/1‑150 (MQ=255)
                                             tGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGt                                                                                                 >  1:2694903/1‑150 (MQ=255)
                                              gTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGtt                                                                                                <  1:1492307/150‑1 (MQ=255)
                                               tCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTg                                                                                               <  2:4387/150‑1 (MQ=255)
                                               tCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTg                                                                                               <  2:2650082/150‑1 (MQ=255)
                                               tCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTg                                                                                               <  2:1597884/150‑1 (MQ=255)
                                                cATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGc                                                                                              <  2:4469758/150‑1 (MQ=255)
                                                 atatCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCt                                                                                             <  2:3586451/150‑1 (MQ=255)
                                                 atatCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCt                                                                                             >  2:340764/1‑150 (MQ=255)
                                                    tCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATg                                                                                          >  2:3067614/1‑150 (MQ=255)
                                                        ccATTTGATTGATAGTCATTTTGACTACTCATTAATGGTAATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTa                                                                                      <  1:1786047/150‑1 (MQ=255)
                                                          aTTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTAtt                                                                                    <  2:2035301/150‑1 (MQ=255)
                                                               attgatAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTgtggt                                                                               >  1:2497204/1‑150 (MQ=255)
                                                               attgatAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTgtggt                                                                               <  2:1117030/150‑1 (MQ=255)
                                                                ttgatAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTAGAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTgtggtg                                                                              <  2:2710517/150‑1 (MQ=255)
                                                                 tgatAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGa                                                                             <  1:103141/150‑1 (MQ=255)
                                                                   atAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGaaa                                                                           <  2:2122631/150‑1 (MQ=255)
                                                                    tAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGaaaa                                                                          <  1:2617857/150‑1 (MQ=255)
                                                                     aGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGaaaaa                                                                         <  2:4062637/150‑1 (MQ=255)
                                                                        cATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATaa                                                                      >  2:3295876/1‑150 (MQ=255)
                                                                          ttttGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAca                                                                    <  1:4125804/150‑1 (MQ=255)
                                                                           tttGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAcat                                                                   <  2:493505/150‑1 (MQ=255)
                                                                           tttGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAcat                                                                   >  1:3542932/1‑150 (MQ=255)
                                                                           tttGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAcat                                                                   >  1:3542981/1‑150 (MQ=255)
                                                                              gACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAcattca                                                                <  2:3361270/150‑1 (MQ=255)
                                                                                ctactCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAcattcatt                                                              >  2:3858460/1‑150 (MQ=255)
                                                                                ctactCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAcattcatt                                                              <  1:2030146/150‑1 (MQ=255)
                                                                                ctactCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAcattcatt                                                              <  1:2780417/150‑1 (MQ=255)
                                                                                  actCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTgg                                                            <  1:3377783/150‑1 (MQ=255)
                                                                                     taTTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCATATAAAAAACTTGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACTCTGAATAAATTGATTTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGtt                                                         <  2:2194805/149‑1 (MQ=255)
                                                                                     cATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGtt                                                         <  1:161534/150‑1 (MQ=255)
                                                                                       ttAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTgg                                                       >  1:2160045/1‑150 (MQ=255)
                                                                                        tAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGt                                                      >  1:3631261/1‑150 (MQ=255)
                                                                                         aaTGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTc                                                     <  2:2826844/150‑1 (MQ=255)
                                                                                         aaTGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTc                                                     >  1:4343365/1‑150 (MQ=255)
                                                                                            gggCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAAc                                                  <  1:306854/150‑1 (MQ=255)
                                                                                              gCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGt                                                <  1:4581324/150‑1 (MQ=255)
                                                                                               cATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTg                                               >  2:521435/1‑150 (MQ=255)
                                                                                               cATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTg                                               >  2:3776242/1‑150 (MQ=255)
                                                                                                 tAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGAc                                             >  2:2440106/1‑150 (MQ=255)
                                                                                                       tatttatAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGaa                                       <  1:2327819/150‑1 (MQ=255)
                                                                                                       tatttatAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGaa                                       <  2:3576712/150‑1 (MQ=255)
                                                                                                         tttatAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGt                                     <  2:712487/150‑1 (MQ=255)
                                                                                                          ttatAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACTTGACTGGGAAGTg                                    >  2:935839/1‑150 (MQ=255)
                                                                                                                  tAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGAttt                            <  1:1925660/150‑1 (MQ=255)
                                                                                                                   aaaaaCAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGAtttt                           >  2:965217/1‑150 (MQ=255)
                                                                                                                   aaaaaCAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGAtttt                           <  1:1124132/150‑1 (MQ=255)
                                                                                                                   aaaaaCAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGAtttt                           <  1:1421867/150‑1 (MQ=255)
                                                                                                                   aaaaaCAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGAtttt                           >  1:3986088/1‑150 (MQ=255)
                                                                                                                    aaaaCAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTc                          >  2:3469385/1‑150 (MQ=255)
                                                                                                                    aaaaCAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTc                          <  1:3962499/150‑1 (MQ=255)
                                                                                                                     aaaCAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTca                         >  2:1445468/1‑150 (MQ=255)
                                                                                                                      aaCAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcac                        <  2:3350819/150‑1 (MQ=255)
                                                                                                                       aCAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcacg                       >  2:2910721/1‑150 (MQ=255)
                                                                                                                        cAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcacgg                      >  1:1800412/1‑150 (MQ=255)
                                                                                                                        cAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcacgg                      >  2:3705766/1‑150 (MQ=255)
                                                                                                                          aTCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcacggca                    >  2:1644895/1‑150 (MQ=255)
                                                                                                                           tCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcacggcac                   >  2:4138201/1‑150 (MQ=255)
                                                                                                                            cAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcacggcacg                  >  2:1493584/1‑150 (MQ=255)
                                                                                                                            cAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcacggcacg                  >  2:504732/1‑150 (MQ=255)
                                                                                                                              gATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGTTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGa                >  1:4201612/1‑150 (MQ=255)
                                                                                                                              gATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGa                <  2:3974811/150‑1 (MQ=255)
                                                                                                                               aTAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGaa               >  1:346743/1‑150 (MQ=255)
                                                                                                                                 aaaaaaCTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAata             >  2:491893/1‑150 (MQ=255)
                                                                                                                                 aaaaaaCTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAata             >  1:1209140/1‑150 (MQ=255)
                                                                                                                                      aCTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGACTATaaaa        >  1:829856/1‑150 (MQ=255)
                                                                                                                                      aCTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATaaaa        <  2:1348590/150‑1 (MQ=255)
                                                                                                                                      aCTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATaaaa        <  2:3397515/150‑1 (MQ=255)
                                                                                                                                       cTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAAc       <  1:837735/150‑1 (MQ=255)
                                                                                                                                        tGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACg      <  2:1005722/150‑1 (MQ=255)
                                                                                                                                        tGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACg      >  1:2407052/1‑150 (MQ=255)
                                                                                                                                        tGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACg      >  2:566661/1‑150 (MQ=255)
                                                                                                                                            aCGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTgc  >  1:4291638/1‑150 (MQ=255)
                                                                                                                                            aCGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTgc  <  2:2619522/150‑1 (MQ=255)
                                                                                                                                            aCGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTCGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTgc  >  2:1775285/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
CGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGC  >  CP009273/2825636‑2825925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGCGCGGCA  >  CP009273/2825636‑2825931
                                                                                                                                                   |                                                                                                                                                    
CGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTG                                                                                                                                                    <  SRR19973073.259666/150‑1 (MQ=60)
CGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTG                                                                                                                                                    <  SRR19973073.259641/150‑1 (MQ=60)
   AAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAA                                                                                                                                                 >  SRR19973073.1031400/1‑150 (MQ=60)
   AAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAA                                                                                                                                                 >  SRR19973073.2719226/1‑150 (MQ=60)
     AAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATT                                                                                                                                               <  SRR19973073.3050994/150‑1 (MQ=60)
      AACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTA                                                                                                                                              >  SRR19973073.3929276/1‑150 (MQ=60)
        CTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGTAATTTGCAATTAGC                                                                                                                                            <  SRR19973073.293021/150‑1 (MQ=60)
        CTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGC                                                                                                                                            <  SRR19973073.969434/150‑1 (MQ=60)
           ATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAG                                                                                                                                         >  SRR19973073.524846/1‑150 (MQ=60)
           ATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAG                                                                                                                                         >  SRR19973073.524922/1‑150 (MQ=60)
            TCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGA                                                                                                                                        <  SRR19973073.3143295/150‑1 (MQ=60)
             CTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGAC                                                                                                                                       <  SRR19973073.2748382/150‑1 (MQ=60)
             CTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGAC                                                                                                                                       <  SRR19973073.2748371/150‑1 (MQ=60)
             CTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGAC                                                                                                                                       <  SRR19973073.2748271/150‑1 (MQ=60)
             CTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGAC                                                                                                                                       <  SRR19973073.2748391/150‑1 (MQ=60)
               TTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACAT                                                                                                                                     >  SRR19973073.1678948/1‑150 (MQ=60)
               TTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACAT                                                                                                                                     >  SRR19973073.1075646/1‑150 (MQ=60)
                TGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATC                                                                                                                                    >  SRR19973073.3687192/1‑150 (MQ=60)
                  CCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTT                                                                                                                                  <  SRR19973073.70466/150‑1 (MQ=60)
                  CCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTT                                                                                                                                  <  SRR19973073.70431/150‑1 (MQ=60)
                  CCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTT                                                                                                                                  <  SRR19973073.70182/150‑1 (MQ=60)
                   CTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTT                                                                                                                                 >  SRR19973073.953238/1‑150 (MQ=60)
                    TCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTT                                                                                                                                <  SRR19973073.797919/150‑1 (MQ=60)
                     CACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTT                                                                                                                               >  SRR19973073.1385764/1‑150 (MQ=60)
                       CTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAG                                                                                                                             <  SRR19973073.2107591/150‑1 (MQ=60)
                       CTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAG                                                                                                                             <  SRR19973073.2107596/150‑1 (MQ=60)
                         GTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAA                                                                                                                           >  SRR19973073.2461044/1‑150 (MQ=60)
                          TCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAAC                                                                                                                          <  SRR19973073.693615/150‑1 (MQ=60)
                            AATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACAC                                                                                                                        <  SRR19973073.625063/150‑1 (MQ=60)
                            AATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACAC                                                                                                                        >  SRR19973073.523761/1‑150 (MQ=60)
                             ATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACG                                                                                                                       >  SRR19973073.1958931/1‑150 (MQ=60)
                             ATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACG                                                                                                                       >  SRR19973073.2605182/1‑150 (MQ=60)
                             ATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACG                                                                                                                       >  SRR19973073.288257/1‑150 (MQ=60)
                                TGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTG                                                                                                                    >  SRR19973073.2770533/1‑150 (MQ=60)
                                   CTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAAT                                                                                                                 >  SRR19973073.3837454/1‑150 (MQ=60)
                                   CTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAAT                                                                                                                 <  SRR19973073.2091166/150‑1 (MQ=60)
                                         ATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTG                                                                                                           <  SRR19973073.3507256/150‑1 (MQ=60)
                                            TTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGG                                                                                                        >  SRR19973073.480156/1‑150 (MQ=60)
                                            TTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGG                                                                                                        >  SRR19973073.973502/1‑150 (MQ=60)
                                             TGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGT                                                                                                       >  SRR19973073.2700397/1‑150 (MQ=60)
                                             TGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGT                                                                                                       >  SRR19973073.2700408/1‑150 (MQ=60)
                                              GTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTT                                                                                                      <  SRR19973073.1495408/150‑1 (MQ=60)
                                              GTCATATCGACCATTTGACTGATCGACATTTTGACTACTCATTAATGGGCATAATTTAAATTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTT                                                                                                      <  SRR19973073.1495375/150‑1 (MQ=60)
                                               TCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTG                                                                                                     >  SRR19973073.1407077/1‑150 (MQ=60)
                                                 ATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCT                                                                                                   >  SRR19973073.3346585/1‑150 (MQ=60)
                                                    TCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCTCGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATG                                                                                                <  SRR19973073.4067481/150‑1 (MQ=60)
                                                    TCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATG                                                                                                <  SRR19973073.4067509/150‑1 (MQ=60)
                                                     CGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGT                                                                                               >  SRR19973073.3336469/1‑150 (MQ=60)
                                                        CCATTTGATTGATAGTCATTTTGACTACTCATTAATGGTAATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTA                                                                                            <  SRR19973073.1789737/150‑1 (MQ=60)
                                                           TTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTG                                                                                         >  SRR19973073.35817/1‑150 (MQ=60)
                                                           TTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTG                                                                                         >  SRR19973073.1489965/1‑150 (MQ=60)
                                                               ATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGT                                                                                     >  SRR19973073.2502366/1‑150 (MQ=60)
                                                                 TGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGA                                                                                   <  SRR19973073.103197/150‑1 (MQ=60)
                                                                 TGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGA                                                                                   <  SRR19973073.2549058/150‑1 (MQ=60)
                                                                 TGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGA                                                                                   <  SRR19973073.103395/150‑1 (MQ=60)
                                                                 TGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGA                                                                                   <  SRR19973073.234282/150‑1 (MQ=60)
                                                                 TGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGA                                                                                   >  SRR19973073.2415972/1‑150 (MQ=60)
                                                                    TAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAA                                                                                <  SRR19973073.2623241/150‑1 (MQ=60)
                                                                    TAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAA                                                                                <  SRR19973073.157333/150‑1 (MQ=60)
                                                                       TCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATA                                                                             <  SRR19973073.4312414/150‑1 (MQ=60)
                                                                          TTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACA                                                                          <  SRR19973073.4134133/150‑1 (MQ=60)
                                                                          TTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACA                                                                          <  SRR19973073.4134058/150‑1 (MQ=60)
                                                                           TTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACAT                                                                         >  SRR19973073.3550077/1‑150 (MQ=60)
                                                                           TTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACAT                                                                         >  SRR19973073.3550035/1‑150 (MQ=60)
                                                                           TTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACAT                                                                         >  SRR19973073.3550028/1‑150 (MQ=60)
                                                                                CTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATT                                                                    <  SRR19973073.2786104/150‑1 (MQ=60)
                                                                                CTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATT                                                                    <  SRR19973073.1792076/150‑1 (MQ=60)
                                                                                CTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATT                                                                    <  SRR19973073.2786084/150‑1 (MQ=60)
                                                                                CTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATT                                                                    <  SRR19973073.2034365/150‑1 (MQ=60)
                                                                                CTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATT                                                                    <  SRR19973073.2034547/150‑1 (MQ=60)
                                                                                  ACTCATTAATGGGCATAATTTTATTTATAGNGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGG                                                                  >  SRR19973073.4285600/1‑150 (MQ=60)
                                                                                  ACTCATTAATGGGCATAATTTTATTTATAGNGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTATTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGG                                                                  >  SRR19973073.4285608/1‑150 (MQ=60)
                                                                                  ACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGG                                                                  <  SRR19973073.3573998/150‑1 (MQ=60)
                                                                                  ACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGG                                                                  <  SRR19973073.3384607/150‑1 (MQ=60)
                                                                                  ACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGG                                                                  <  SRR19973073.3384584/150‑1 (MQ=60)
                                                                                  ACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGG                                                                  >  SRR19973073.115724/1‑150 (MQ=60)
                                                                                     CATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTT                                                               <  SRR19973073.161920/150‑1 (MQ=60)
                                                                                       TTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGG                                                             >  SRR19973073.2164553/1‑150 (MQ=60)
                                                                                        TAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGT                                                            >  SRR19973073.3638550/1‑150 (MQ=60)
                                                                                         AATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTC                                                           >  SRR19973073.4352052/1‑150 (MQ=60)
                                                                                           TGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAA                                                         >  SRR19973073.1646556/1‑150 (MQ=60)
                                                                                            GGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAAC                                                        <  SRR19973073.307494/150‑1 (MQ=60)
                                                                                            GGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAAC                                                        <  SRR19973073.307974/150‑1 (MQ=60)
                                                                                             GGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACG                                                       <  SRR19973073.1251162/150‑1 (MQ=60)
                                                                                              GCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGT                                                      <  SRR19973073.4590503/150‑1 (MQ=60)
                                                                                              GCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGT                                                      <  SRR19973073.4590481/150‑1 (MQ=60)
                                                                                              GCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGT                                                      <  SRR19973073.4590453/150‑1 (MQ=60)
                                                                                                       TATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAA                                             <  SRR19973073.2332567/150‑1 (MQ=60)
                                                                                                          TTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTG                                          >  SRR19973073.1077057/1‑150 (MQ=60)
                                                                                                          TTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTG                                          <  SRR19973073.731262/150‑1 (MQ=60)
                                                                                                           TATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGC                                         >  SRR19973073.642104/1‑150 (MQ=60)
                                                                                                           TATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGC                                         <  SRR19973073.1834924/150‑1 (MQ=60)
                                                                                                           TATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGC                                         >  SRR19973073.641936/1‑150 (MQ=60)
                                                                                                                 GTAAAAACAATCAGATAAAGACCTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATT                                   <  SRR19973073.899705/150‑1 (MQ=60)
                                                                                                                  TAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTT                                  <  SRR19973073.1929483/150‑1 (MQ=60)
                                                                                                                  TAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTT                                  <  SRR19973073.4557587/150‑1 (MQ=60)
                                                                                                                  TAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTT                                  <  SRR19973073.1929574/150‑1 (MQ=60)
                                                                                                                   AAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTT                                 <  SRR19973073.1424862/150‑1 (MQ=60)
                                                                                                                   AAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTT                                 >  SRR19973073.3994034/1‑150 (MQ=60)
                                                                                                                   AAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTT                                 >  SRR19973073.3994015/1‑150 (MQ=60)
                                                                                                                   AAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTT                                 <  SRR19973073.1126424/150‑1 (MQ=60)
                                                                                                                    AAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTC                                <  SRR19973073.3503217/150‑1 (MQ=60)
                                                                                                                    AAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTC                                <  SRR19973073.3970389/150‑1 (MQ=60)
                                                                                                                     AAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCA                               <  SRR19973073.2318710/150‑1 (MQ=60)
                                                                                                                        CAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGG                            >  SRR19973073.1804128/1‑150 (MQ=60)
                                                                                                                         AATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGC                           >  SRR19973073.571692/1‑150 (MQ=60)
                                                                                                                            CAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACG                        >  SRR19973073.3876784/1‑150 (MQ=60)
                                                                                                                              GATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGTTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGA                      >  SRR19973073.4209954/1‑150 (MQ=60)
                                                                                                                               ATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAA                     >  SRR19973073.347441/1‑150 (MQ=60)
                                                                                                                                 AAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATA                   >  SRR19973073.1211655/1‑150 (MQ=60)
                                                                                                                                      ACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGACGTGCGTGATTTTCACGGCACGGAATATAAAA              >  SRR19973073.1249811/1‑150 (MQ=60)
                                                                                                                                      ACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGACTATAAAA              >  SRR19973073.831561/1‑150 (MQ=60)
                                                                                                                                      ACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAA              >  SRR19973073.831522/1‑150 (MQ=60)
                                                                                                                                      ACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAA              >  SRR19973073.831608/1‑150 (MQ=60)
                                                                                                                                       CTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTTTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATTTAAAAC             <  SRR19973073.839148/150‑1 (MQ=60)
                                                                                                                                       CTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAAC             <  SRR19973073.839458/150‑1 (MQ=60)
                                                                                                                                       CTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAAC             <  SRR19973073.2266647/150‑1 (MQ=60)
                                                                                                                                        TGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAATATAACATTCATTGGGTTGGTCAACGTGACTGGGACGTGCGGGATTTTCACGGCACGGAATATAAAACG            >  SRR19973073.3004798/1‑150 (MQ=60)
                                                                                                                                        TGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACG            >  SRR19973073.2412006/1‑150 (MQ=60)
                                                                                                                                            ACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGC        >  SRR19973073.4300179/1‑150 (MQ=60)
                                                                                                                                                  TTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGACTATAAAACGCTGCGCGGCA  >  SRR19973073.3735046/1‑150 (MQ=60)
                                                                                                                                                  TTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGCGCGGCA  <  SRR19973073.2383670/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
CGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGCGCGGCA  >  CP009273/2825636‑2825931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑