Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,825,783 C→T 100% intergenic (‑135/‑52) norR ← / → norV anaerobic nitric oxide reductase DNA‑binding transcriptional activator/anaerobic nitric oxide reductase flavorubredoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,825,7830CT96.8% 658.0 / 7.9 187intergenic (‑135/‑52)norR/norVanaerobic nitric oxide reductase DNA‑binding transcriptional activator/anaerobic nitric oxide reductase flavorubredoxin
Reads supporting (aligned to +/- strand):  ref base C (4/2);  new base T (91/90);  total (95/92)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 187 total aligned reads displayed.

AAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGCGCGG  >  CP009273/2825640‑2825929
                                                                                                                                               |                                                                                                                                                  
aaaaCTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAAt                                                                                                                                              <  2:4721657/150‑1 (MQ=255)
aaaaCTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAAt                                                                                                                                              <  2:4721649/150‑1 (MQ=255)
aaaaCTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAAt                                                                                                                                              <  1:1834567/150‑1 (MQ=255)
 aaaCTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAAtt                                                                                                                                             <  2:2200867/150‑1 (MQ=255)
  aaCTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTa                                                                                                                                            <  1:3895382/150‑1 (MQ=255)
  aaCTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTa                                                                                                                                            <  2:724170/150‑1 (MQ=255)
   aCTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAg                                                                                                                                           <  1:3172669/150‑1 (MQ=255)
    cTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGc                                                                                                                                          <  1:3588335/150‑1 (MQ=255)
      cATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCaa                                                                                                                                        <  2:2043274/150‑1 (MQ=255)
          tttGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACa                                                                                                                                    >  2:4023239/1‑150 (MQ=255)
           ttGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACAt                                                                                                                                   >  2:1096477/1‑150 (MQ=255)
           ttGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACAt                                                                                                                                   >  2:1469421/1‑150 (MQ=255)
             gCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCt                                                                                                                                 <  1:3686365/150‑1 (MQ=255)
             gCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCt                                                                                                                                 <  1:4293218/150‑1 (MQ=255)
             gCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCt                                                                                                                                 <  1:1126720/150‑1 (MQ=255)
              ccTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCtt                                                                                                                                <  1:190608/150‑1 (MQ=255)
                tCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCACTTTGCAATTAGCAAGACATCtttt                                                                                                                              >  2:4035421/1‑150 (MQ=255)
                tCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCtttt                                                                                                                              <  2:3610512/150‑1 (MQ=255)
                tCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCtttt                                                                                                                              <  2:2899498/150‑1 (MQ=255)
                   cTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAg                                                                                                                           <  2:4101542/150‑1 (MQ=255)
                    tGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAAGTAGCAAGACATCTTTTTAGa                                                                                                                          >  2:2916954/1‑150 (MQ=255)
                       cAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAaca                                                                                                                       >  2:2826890/1‑150 (MQ=255)
                        aaTTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAacac                                                                                                                      >  1:2997484/1‑150 (MQ=255)
                          tttGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGc                                                                                                                    >  2:1389859/1‑150 (MQ=255)
                             gACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGa                                                                                                                 <  1:2202338/150‑1 (MQ=255)
                             gACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGa                                                                                                                 <  1:578217/150‑1 (MQ=255)
                             gACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGa                                                                                                                 <  2:1235515/150‑1 (MQ=255)
                             gACTATAGAGAGTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCACGACATCTTTTTAGAACACGCTGa                                                                                                                 <  2:3706275/150‑1 (MQ=255)
                               cTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAAt                                                                                                               <  1:3166694/150‑1 (MQ=255)
                                tataGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATa                                                                                                              <  1:2110005/150‑1 (MQ=255)
                                  taGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGAAATACATCTTTTTAGAACACGCTGAATaaa                                                                                                            >  2:324707/1‑150 (MQ=255)
                                   aGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAAt                                                                                                           >  1:3448752/1‑150 (MQ=255)
                                   aGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAAt                                                                                                           >  1:586783/1‑150 (MQ=255)
                                   aGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAAt                                                                                                           >  2:4004932/1‑150 (MQ=255)
                                      tatTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGa                                                                                                        <  2:165949/150‑1 (MQ=255)
                                          gTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGtt                                                                                                    >  1:880919/1‑150 (MQ=255)
                                          gTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGtt                                                                                                    <  2:1414279/150‑1 (MQ=255)
                                          gTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGtt                                                                                                    <  2:3164061/150‑1 (MQ=255)
                                          gTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGtt                                                                                                    >  2:4177016/1‑150 (MQ=255)
                                           tCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTg                                                                                                   >  1:1914341/1‑150 (MQ=255)
                                            cATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGc                                                                                                  <  2:1764165/150‑1 (MQ=255)
                                             atatCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCt                                                                                                 >  2:1448773/1‑150 (MQ=255)
                                             atatCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCt                                                                                                 >  2:1448935/1‑150 (MQ=255)
                                             atatCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCt                                                                                                 >  2:866013/1‑150 (MQ=255)
                                             atatCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCt                                                                                                 >  2:1375341/1‑150 (MQ=255)
                                               atCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTAt                                                                                               <  1:2612513/150‑1 (MQ=255)
                                               atCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTAt                                                                                               <  1:2612516/150‑1 (MQ=255)
                                                tCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATg                                                                                              <  1:2972263/150‑1 (MQ=255)
                                                 cGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCACTTTGCAATTAGCACGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGt                                                                                             >  2:2646580/1‑150 (MQ=255)
                                                  gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTc                                                                                            >  1:1440846/1‑150 (MQ=255)
                                                  gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTc                                                                                            >  2:1291258/1‑150 (MQ=255)
                                                     cATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTAt                                                                                         <  1:710475/150‑1 (MQ=255)
                                                      aTTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTAtt                                                                                        >  1:2885873/1‑150 (MQ=255)
                                                         tgattgatAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATtgtg                                                                                     <  2:3305218/150‑1 (MQ=255)
                                                             tgatAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGa                                                                                 <  2:4079953/150‑1 (MQ=255)
                                                             tgatAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGa                                                                                 <  2:2214914/150‑1 (MQ=255)
                                                                tAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTCTGTCTATTGTGGTGaaaa                                                                              >  1:1266135/1‑150 (MQ=255)
                                                                  gTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAAt                                                                            <  1:1864099/150‑1 (MQ=255)
                                                                    cATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATaa                                                                          <  2:4410694/150‑1 (MQ=255)
                                                                    cATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATaa                                                                          <  2:3389751/150‑1 (MQ=255)
                                                                    cATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATaa                                                                          >  1:3387430/1‑150 (MQ=255)
                                                                     aTTTTGACTACTCATTAATGGTCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAc                                                                         <  2:2590270/150‑1 (MQ=255)
                                                                     aTTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAc                                                                         <  2:406053/150‑1 (MQ=255)
                                                                      ttttGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAca                                                                        >  2:691861/1‑150 (MQ=255)
                                                                       tttGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAcat                                                                       <  1:4526477/150‑1 (MQ=255)
                                                                       tttGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAcat                                                                       <  1:4526411/150‑1 (MQ=255)
                                                                          gACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAcattca                                                                    >  1:1359259/1‑150 (MQ=255)
                                                                           actactCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAcattcat                                                                   <  1:4118966/150‑1 (MQ=255)
                                                                             tactCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAACAATAACATTCATTg                                                                 >  2:2685140/1‑150 (MQ=255)
                                                                                tCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGt                                                              <  1:978698/150‑1 (MQ=255)
                                                                                  aTTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTg                                                            >  2:2076540/1‑150 (MQ=255)
                                                                                   ttAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTgg                                                           >  1:36284/1‑150 (MQ=255)
                                                                                   ttAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTgg                                                           >  2:3018103/1‑150 (MQ=255)
                                                                                   ttAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTgg                                                           >  2:631003/1‑150 (MQ=255)
                                                                                   ttAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTgg                                                           >  1:36299/1‑150 (MQ=255)
                                                                                       tGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCaa                                                       >  1:357485/1‑150 (MQ=255)
                                                                                            aTAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGa                                                  >  2:4629075/1‑150 (MQ=255)
                                                                                               aTTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTg                                               >  2:490676/1‑150 (MQ=255)
                                                                                                   tatttatAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGaa                                           <  2:3500275/150‑1 (MQ=255)
                                                                                                     tttatAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGt                                         >  1:2304259/1‑150 (MQ=255)
                                                                                                      ttatAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTAGGTCAACGTGACTGGGAAGTg                                        >  1:3633799/1‑150 (MQ=255)
                                                                                                        ataGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCg                                      >  1:2041604/1‑150 (MQ=255)
                                                                                                          agagTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTg                                    <  2:841900/150‑1 (MQ=255)
                                                                                                            agTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGAt                                  >  2:3097275/1‑150 (MQ=255)
                                                                                                             gTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGAtt                                 >  1:1533469/1‑150 (MQ=255)
                                                                                                             gTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGAtt                                 <  1:3237919/150‑1 (MQ=255)
                                                                                                               aaaaaCAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGAtttt                               <  1:366953/150‑1 (MQ=255)
                                                                                                               aaaaaCAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGAtttt                               >  2:11891/1‑150 (MQ=255)
                                                                                                                            tAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAat                  <  1:3446650/150‑1 (MQ=255)
                                                                                                                            tAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAat                  <  1:225006/150‑1 (MQ=255)
                                                                                                                            tAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAat                  <  1:3447740/150‑1 (MQ=255)
                                                                                                                             aaaaaaCTGGCATGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAata                 >  1:4587902/1‑150 (MQ=255)
                                                                                                                              aaaaaCTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAatat                >  1:2904102/1‑150 (MQ=255)
                                                                                                                                  aCTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATaaaa            <  2:3846691/150‑1 (MQ=255)
                                                                                                                                    tGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACg          <  2:1863138/150‑1 (MQ=255)
                                                                                                                                       cACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTg       >  2:2880754/1‑150 (MQ=255)
                                                                                                                                        aCGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTgc      >  1:2272501/1‑150 (MQ=255)
                                                                                                                                           cAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGcgcg   <  2:1981119/150‑1 (MQ=255)
                                                                                                                                            aaTTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGCGCgg  >  2:328343/1‑150 (MQ=255)
                                                                                                                                            aaTTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGCGCgg  >  2:1571104/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
AAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGCGCGG  >  CP009273/2825640‑2825929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGCGCGGC  >  CP009273/2825634‑2825930
                                                                                                                                                     |                                                                                                                                                   
AACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATT                                                                                                                                                     <  SRR19973078.3116356/150‑1 (MQ=60)
AACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATT                                                                                                                                                     <  SRR19973078.943423/150‑1 (MQ=60)
AACGGAAAAACTCATCTTGGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATT                                                                                                                                                     <  SRR19973078.3117185/150‑1 (MQ=60)
      AAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAAT                                                                                                                                               <  SRR19973078.1845591/150‑1 (MQ=60)
      AAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAAT                                                                                                                                               <  SRR19973078.4263303/150‑1 (MQ=60)
        AACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTA                                                                                                                                             <  SRR19973078.3917576/150‑1 (MQ=60)
         ACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAG                                                                                                                                            <  SRR19973078.3190260/150‑1 (MQ=60)
         ACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAG                                                                                                                                            <  SRR19973078.3190338/150‑1 (MQ=60)
          CTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGC                                                                                                                                           <  SRR19973078.3607450/150‑1 (MQ=60)
          CTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGC                                                                                                                                           <  SRR19973078.3608483/150‑1 (MQ=60)
                   GCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCT                                                                                                                                  <  SRR19973078.4317535/150‑1 (MQ=60)
                   GCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCT                                                                                                                                  <  SRR19973078.3707446/150‑1 (MQ=60)
                   GCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCT                                                                                                                                  <  SRR19973078.1133521/150‑1 (MQ=60)
                    CCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTT                                                                                                                                 <  SRR19973078.192185/150‑1 (MQ=60)
                     CTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTT                                                                                                                                <  SRR19973078.607314/150‑1 (MQ=60)
                         CTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAG                                                                                                                            <  SRR19973078.2160145/150‑1 (MQ=60)
                              AATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACAC                                                                                                                       >  SRR19973078.3014572/1‑150 (MQ=60)
                               ATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACG                                                                                                                      <  SRR19973078.3177823/150‑1 (MQ=60)
                                  gaacttcgctaacgcctgagtcagggagtgaaaTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTG                                                                                                                   <  SRR19973078.4589176/117‑1 (MQ=60)
                                   GACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGA                                                                                                                  <  SRR19973078.2215208/150‑1 (MQ=60)
                                   GACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGA                                                                                                                  <  SRR19973078.582036/150‑1 (MQ=60)
                                   GACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGA                                                                                                                  <  SRR19973078.582040/150‑1 (MQ=60)
                                     CTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAAT                                                                                                                <  SRR19973078.3184353/150‑1 (MQ=60)
                                      TATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATA                                                                                                               <  SRR19973078.2122315/150‑1 (MQ=60)
                                      TATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATA                                                                                                               <  SRR19973078.2122403/150‑1 (MQ=60)
                                         AGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAAT                                                                                                            >  SRR19973078.3468448/1‑150 (MQ=60)
                                         AGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAAT                                                                                                            >  SRR19973078.3468306/1‑150 (MQ=60)
                                         AGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAAT                                                                                                            >  SRR19973078.590647/1‑150 (MQ=60)
                                                GTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTT                                                                                                     >  SRR19973078.886345/1‑150 (MQ=60)
                                                GTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTT                                                                                                     >  SRR19973078.1416729/1‑150 (MQ=60)
                                                 TCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTG                                                                                                    >  SRR19973078.1925640/1‑150 (MQ=60)
                                                    TATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGGGGTTGCTA                                                                                                 <  SRR19973078.2971011/150‑1 (MQ=60)
                                                     ATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTAT                                                                                                <  SRR19973078.2627444/150‑1 (MQ=60)
                                                     ATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTAT                                                                                                <  SRR19973078.2627447/150‑1 (MQ=60)
                                                      TCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATG                                                                                               <  SRR19973078.2989223/150‑1 (MQ=60)
                                                      TCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATG                                                                                               <  SRR19973078.2990156/150‑1 (MQ=60)
                                                        GACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTC                                                                                             >  SRR19973078.1449719/1‑150 (MQ=60)
                                                        GACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTA‑TTATAGAGTAAAAACAATCAGATAAAAAAATGGCACGCAATTTGCAATTAGCAAGACGTCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCT                                                                                            >  SRR19973078.1450498/1‑150 (MQ=60)
                                                           CATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTAT                                                                                          <  SRR19973078.714840/150‑1 (MQ=60)
                                                            ATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATT                                                                                         >  SRR19973078.2902273/1‑150 (MQ=60)
                                                            ATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATT                                                                                         <  SRR19973078.4367586/150‑1 (MQ=60)
                                                             TTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTG                                                                                        <  SRR19973078.18620/150‑1 (MQ=60)
                                                                 ATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGT                                                                                    >  SRR19973078.907904/1‑150 (MQ=60)
                                                                      TAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTCTGTCTATTGTGGTGAAAA                                                                               >  SRR19973078.1273997/1‑150 (MQ=60)
                                                                        GTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAAT                                                                             <  SRR19973078.1875241/150‑1 (MQ=60)
                                                                        GTCATTTTGAATACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAAT                                                                             <  SRR19973078.1876830/150‑1 (MQ=60)
                                                                          CATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAA                                                                           >  SRR19973078.3406817/1‑150 (MQ=60)
                                                                          CATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAA                                                                           <  SRR19973078.4573296/150‑1 (MQ=60)
                                                                             TTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACAT                                                                        <  SRR19973078.4552044/150‑1 (MQ=60)
                                                                             TTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACAT                                                                        <  SRR19973078.4552111/150‑1 (MQ=60)
                                                                             TTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACAT                                                                        <  SRR19973078.487310/150‑1 (MQ=60)
                                                                                GACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCA                                                                     >  SRR19973078.278970/1‑150 (MQ=60)
                                                                                GACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCA                                                                     >  SRR19973078.1367742/1‑150 (MQ=60)
                                                                                 ACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCAT                                                                    <  SRR19973078.4142493/150‑1 (MQ=60)
                                                                                      TCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGT                                                               <  SRR19973078.1689151/150‑1 (MQ=60)
                                                                                      TCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGT                                                               <  SRR19973078.984816/150‑1 (MQ=60)
                                                                                        ATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTG                                                             >  SRR19973078.989762/1‑150 (MQ=60)
                                                                                         TTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGG                                                            >  SRR19973078.36704/1‑150 (MQ=60)
                                                                                         TTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGG                                                            >  SRR19973078.36719/1‑150 (MQ=60)
                                                                                         TTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGG                                                            >  SRR19973078.37253/1‑150 (MQ=60)
                                                                                         TTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGG                                                            >  SRR19973078.38563/1‑150 (MQ=60)
                                                                                             TGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAA                                                        >  SRR19973078.359722/1‑150 (MQ=60)
                                                                                                GCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGT                                                     >  SRR19973078.3195726/1‑150 (MQ=60)
                                                                                                           TTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGT                                          >  SRR19973078.2317469/1‑150 (MQ=60)
                                                                                                           TTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGT                                          >  SRR19973078.2317473/1‑150 (MQ=60)
                                                                                                            TTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTG                                         >  SRR19973078.3653948/1‑150 (MQ=60)
                                                                                                            TTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTAGGTCAACGTGACTGGGAAGTG                                         >  SRR19973078.3654444/1‑150 (MQ=60)
                                                                                                              ATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCG                                       >  SRR19973078.2053578/1‑150 (MQ=60)
                                                                                                                   GTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATT                                  >  SRR19973078.3633163/1‑150 (MQ=60)
                                                                                                                   GTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATT                                  >  SRR19973078.1542675/1‑150 (MQ=60)
                                                                                                                   GTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATT                                  >  SRR19973078.1947702/1‑150 (MQ=60)
                                                                                                                   GTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATT                                  >  SRR19973078.2283616/1‑150 (MQ=60)
                                                                                                                   GTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATT                                  <  SRR19973078.3256149/150‑1 (MQ=60)
                                                                                                                     AAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTT                                >  SRR19973078.358668/1‑150 (MQ=60)
                                                                                                                     AAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTT                                <  SRR19973078.369216/150‑1 (MQ=60)
                                                                                                                      AAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTC                               >  SRR19973078.4072947/1‑150 (MQ=60)
                                                                                                                             TCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCAC                        >  SRR19973078.246761/1‑150 (MQ=60)
                                                                                                                                 ATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAA                    >  SRR19973078.343118/1‑150 (MQ=60)
                                                                                                                                 ATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAA                    <  SRR19973078.847733/150‑1 (MQ=60)
                                                                                                                                  TAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAAT                   <  SRR19973078.3466330/150‑1 (MQ=60)
                                                                                                                                  TAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAAT                   <  SRR19973078.3466331/150‑1 (MQ=60)
                                                                                                                                  TAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAAT                   <  SRR19973078.3467423/150‑1 (MQ=60)
                                                                                                                                  TAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAAT                   <  SRR19973078.226786/150‑1 (MQ=60)
                                                                                                                                  TAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAAT                   >  SRR19973078.2259297/1‑150 (MQ=60)
                                                                                                                                   AAAAAACTGGCATGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATA                  >  SRR19973078.4613896/1‑150 (MQ=60)
                                                                                                                                    AAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATAT                 >  SRR19973078.2920647/1‑150 (MQ=60)
                                                                                                                                    AAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATAT                 >  SRR19973078.2920619/1‑150 (MQ=60)
                                                                                                                                             CACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTG        <  SRR19973078.1335428/150‑1 (MQ=60)
                                                                                                                                              ACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGC       >  SRR19973078.2285615/1‑150 (MQ=60)
                                                                                                                                                  AATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGCGCGG   >  SRR19973078.3676049/1‑150 (MQ=60)
                                                                                                                                                   ATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGACTATAAAACGCTGCGCGGC  >  SRR19973078.1430852/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
AACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGCGCGGC  >  CP009273/2825634‑2825930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑